presents

The answers in questions #3 and #4

Question #3
yMost Laboratory media contains a

fermentable carbohydrates and peptone because the majority of bacteria requires carbon, nitrogen and energy source in these forms. How are these three needs met by glucose-minimal salt medium?

But yWhat is a glucose-minimal media?

MINIMAL MEDIA
y are those that contain the minimum, nutrients

possible for colony growth, generally without the presence of amino acids , and are often used to grow wild types of microorganisms. y can be also used to select for or against recombinants or exconjugants.

usually contains ..
y a carbon source for bacterial growth which may be

glucose, or a less energy-rich source such as succinate y various salt which may vary among bacteria species and growing conditions that generally provide essential elements such as magnesium, nitrogen, phosphorus and sulfur to allow bacteria to synthesize protein and nucleic acid. Ammonium chloride is commonly used, particularly given that the majority of the bacterial species cannot fix nitrogen.

and . yIons or electrolytes- such as sodium, chloride, potassium, calcium, phosphorus, and magnesium are also needed by the bacteria.

Question #4
y Flask A contains yeast cells in glucose-minimal salt

broth incubated at 30 degrees with aeration. Flask B contains yeast cell in glucose minimal salt broth incubated at 3o degrees in an anaerobic jar. the yeast are the facultative anaerobes. a. Which culture produced more atp? b. Which culture produced more alcohol? c. Which culture has a shorter generation time? d. Which culture has the greater cell mass? e. Which culture has the higher absorbance?

a. Which culture produced more atp?
y flasks A the oxidation of glucose in aerobic respiration

is.. C6H12O6 + 6oxygen + 38 ADP + 38 PHOSPHATE ---6CO2 +6H20 +38 ATP y Because the facultative anaerobe which uses fermentation pathway that produced only 2 atp. y formula: glucose + 2ADP + 2 phosphate --- 2 C6H5OH + 2 CO2 + 2 ATP.

b. Which culture produced more alcohol?

yflask B because

fermentation occur in the absence of oxygen produces 2 ethanol

c. Which culture had a shorter generation time?
y bacteria are said to be aerobic f they require O2 and

grown best at higher oxygen tension usually 20 percent or more, but microaerophilic bacteria needs O2 but grows best or reduced O2 tension less than 1o percent. y Facultative bacteria that makes ATP by aerobic respiration if oxygen is present but is also capable of switching to fermentation, therefore, the culture in Flask A has a shorter generation time.

d. Which culture has the greater cell mass?

yFlask A, because it

has a shorter generation time.

e. Which culture had the higher absorbance?

yFlask A because it s

environment is open where oxygen is available.

The end .
yProduced by:
y Aubrey

Rose W. Kingco y Aljeric K. Amorganda y Jezreel Lardizabal y Sitti Jurna Ahkmad y Jeriza Idji y Rezwana Sulayman

Thankz for watching Hope you learn something ^________^

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