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Complete Amna Marie

Randomized Siti Nurbaiti


Windy
Design Yustina C.F.S

Unequal Replication
Scientific Method

Problem Observation Hypothesis Experiment Conclusion


EXPERIMENTAL DESIGN

A Planning process of an experiment to get statistically


analyzed data to constructan objective and valid conclusion
(Montgomery, 2005) To minimising errors
What kind of design?
Sample design (amount of sample, plot, etc)
Instrument design (shape, size, etc)
Administration design (resarch cost)
Analysis design (statistically)
Statistically, in biologycal experiment, we
use PARAMETRIC STATISTIC method

Parametric
Statistic

1 Sample 2 Samples > 2 samples

One Sample 2 or more


Paired Unpaired 1 factor
T test factor

Paired Independent One Way Two way


sample T test sample T test ANOVA ANOVA
Basic principles in experimental design

Randomization

Basic Exp.
Replication
Design

Blocking
Based on those 3 basic principles, we can
divide statistic experimental design into:

CRD with equal


replication

Complete
Randomized CRD with unequal
Design (CRD); replication
Indonesia: RAL)
Stat. Exp. Design
Complete
Incomplete
Randomized Block
Randomized
Design (CRBD;
Design
Indonesia: RAKL)
CRD with unequal replication

CRD is commonly used for studies where the experimental material


makes it difficult to use an equal number of replications for all
treatments. Some examples of these are:
Animal feeding experiments where the number of animals for each breed is
not the same
Experiments for comparing body lenght of diffrent species of insect caught
in an insect trap
Experiments that are originally set up with an equal number of replications
but some experimental units are likely to be lost or destroyed during
experimentation
Page 19,
Example with SPSS Gomes and Gomes
Running
General
Linear
Mode

Univariate
Analisis One-way ANOVA
Result Analysis

ANOVA

What does it
means?

GLM-
Univariate
Treatment Sum of Square Mean Suare
Degree of Freedom F value
(Perlakuan) (Jumlah Kuadrat) (Kuadrat Tengah)
(derajat bebas) (F hitung)

Significancy
(signifikansi)

Experimental
Error (Galat)

General Value
(Umum)
SPSS

Manual
H0 : There is no signficance effect from the factor to the

How to Conclude result


H1: There is significance effect from the factor to the
result

SIGNIFICANCY LEVEL > 0,05


F value > F table 1 %
So H0 is accepted
very significance
But if,
F table 5 % <F value< F table 1 %
SIGNIFICANCY LEVEL< 0,05
there is significance effect
So, H0 is rejected which means H1 is accepted
F value < F table 5 %
There is no significancy

F1= db perlakuan = t-1; F2= db galat=n(t-1)


F table 1% = 2,18 F tabel 5%=3,00

Based on the result sig < 0,005 and F value= 8,55 (more than F table 1%) , we can conclude that,
There is a very significance effect from kind of herbisides, herbicides dosages and aplication time on the
growth of Oryza sativa
EXCERCISE
LEVEL UP
1
Tejo is a student of animal physiology laboratory. For this latest semester he has
conducts an experiment on Gurame fish. He used 11 pools with the wides about 100
m2/pool, and gave 3 treatments (variance of total density of juveniles sowed) about
(a) 5 kg (37 fish); (b) 10 kg(74 fish) and (c) 20 kg (148 fish). In this experiment, Tejo has
replicates the treatment 4 times for a and b and 3 times replication on c. From the
total of body weight measured (gr) from each pool Tejo has took the datas (mean):
Replication Treatments (kg/100m2) Total
5 10 20
1 382,5 350,5 285,0
2 379,6 345,6 276,3
3 380,0 351,3 280,7
4 375,9 340,6 -
Total 1518,0 1338,0 842,0 3748
Mean 379,5 347,0 280,7 340,7
Please help Tejo to analyse the data!
Conclusion:
Perlakuan penebaran benih memberikan hasil yang sangat signifikan terhadap bobot tubuh
ikan gurame
Conclusion

To conduct the experience with many total amount of


sample and more than two independent variable, we can
use one way ANOVA (1 factor included) or two way
ANOVA (equals or more than 2 factors)
The differences between CRD with Equal replication and
CRD with unequal replication are: total replication of each
methods, number of sample/treatment, total of dF, and the
different statistic analysis method using SPSS (Welchs test).
There is no differences in F value between manual
calculation method and SPSS calculation method
References

Cronk. B.C., 2003. How to use SPSS- Step by step guide to analysis and
interpretation. 4th ed. Pyrczak Publishing. Glendale
Montgomery, D.C., G.C Runger. 2005. Applied Statistics and Probability for
Engineers. John Wiley and Sons. Hoboken
Gomez A., and K.A Gomez. 2010. Prosedur Statistik Untuk Penelitian
Pertanian. Penerbit Universitas Indonesia. Jakarta
Griffith, A., 2007. SPSS For Dummies- A Reference for the rest of us. Wiley
Publishing. Indiana.
Thank you