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Webinar: Improving your results

with MasterPlex QT
This presentation accompanies the free webinar recording at:
https://www1.gotomeeting.com/register/351125992
July 29, 2010
Presenters: Robert Lynde
Deputy Director
Sales Operations & Strategic Alliances Manager

Allen Liu
Supervisor
Technical Support & Project Management
© Hitachi Software Engineering America, Ltd. 2010. All rights reserved.
Topics
1) A close up look at the 4-PL and 5-PL model equations
2) Heteroscedasticity & Weighting
3) BONUS: What’s New in MasterPlex QT 2010
4) Ensuring the quality of your data with the Quality Control
Manager
5) How to use time-saving features such as templates & Best Fit

© Hitachi Software Engineering America, Ltd. 2010. All rights reserved.


4 Parameter Logistic (4PL)

• F(x) = ((A-D)/(1+((x/C)^B))) + D
• Nonlinear regression
• Monotonic
– e.g. For every MFI, there is only one
concentration
• Symmetrical around the midpoint or
inflection point

© Hitachi America, Ltd. 2010. All rights reserved.


4 Parameter Logistic (4PL)

> high standard OR highest extrapolated value

D = maximum asymptote

B = slope factor/steepness of curve


C = inflection point

A = minimum asymptote
< low standard OR lowest extrapolated value
© Hitachi America, Ltd. 2010. All rights reserved.
Caution on Extrapolation

tiny change in MFI = HUGE change in concentration


© Hitachi America, Ltd. 2010. All rights reserved.
5 Parameter Logistic (5PL)

• F(x) = A + (D/(1+(X/C)^B)^E)
• Nonlinear regression
• Monotonic
• Key difference = asymmetry
• E = asymmetry factor
• E = 1 = 4PL model equation

© Hitachi America, Ltd. 2010. All rights reserved.


5 Parameter Logistic (5PL)

© Hitachi America, Ltd. 2010. All rights reserved.


Assumptions of the 4PL & 5PL

• 2 assumptions about how the data are


scattered around the curve…
1. The scatter follows a Gaussian or “normal”
distribution
2. The standard deviation of the scatter (of the
response values or MFI) is the same for all
values of x (or concentrations in our case)

© Hitachi America, Ltd. 2010. All rights reserved.


Heteroscedasticity

© Hitachi America, Ltd. 2010. All rights reserved.


Weighting

• 1/Y^2 (Relative weighting) – Most commonly


used
• 1/Y – Most effective when the scatter follows a
Poisson distribution
• Mainly affects points on the lower portion of
the curve
• Test by doing before/after on the % Recovery

© Hitachi America, Ltd. 2010. All rights reserved. 1


What’s new for MasterPlex QT 2010?
• Expanded Platform Support
• Meso Scale Discovery (MSD) platform compatibility
• Improved Data Integrity
• Built-in Quality Control Manager
• Added new Upper Limit of Detection calculation for QC
Manager
• Further Time-saving
• Auto calculation for plate templates
• Dose Response Analysis
• EC or IC50 calculation
• EC or IC(anything) calculation
• Enhanced Reporting
• Merge group wells in the Data Table
• Customizable standard curve chart titles & x/y-axes
© Hitachi Software Engineering America, Ltd. 2010. All rights reserved.
Resources
• Free 14-Day Trial: http://www.MiraiBio.com/QT
• Support: support@miraibio.com
• Sales: info@miraibio.com
• MiraiBio Support Menu
• Community
• Knowledge Base
• Blog
• Webinars: http://www.MiraiBio.com/Webinars

© Hitachi Software Engineering America, Ltd. 2010. All rights reserved.


© Hitachi America, Ltd. 2010. All rights reserved. 1