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Enterobacteriaceae

Wilda Mahdani
Mikrobiologi Klinik FK-USK, RSUDZA
Famili Enterobacteriaceae
Batang Gram negatif, tidak berspora, fakultatif
anaerob
Free living : tanah, air
buah2an, sayuran, biji2an,
tumbuhan, cacing, serangga, (usus)
hewan danmanusia
Famili Enterobacteriaceae
Morfologi : 0,3 1,8 u, single, flagella + / -
Pertumbuhan : suhu optimum 37oC
Biokimia : fermentatif / respiratif
memecah glukosa
mereduksi nitrat
oksidase -, katalase +
Famili Enterobacteriaceae
Diferensiasi dari famili lain didasarkan pada :

* morfologi : ukuran, bentuk (rounded


end), flagella (peritrichous)
* pewarnaan Gram
* pertumbuhan : koloni, kebutuhan nutrisi
* sifat biokimia
* patogenitas
Klassifikasi Bergey (1974)
Tribe I Escherichieae : Escherichia, Shiegella,
Edwardsiella, Salmonella, Citrobacter
Tribe II Klebsiella : Klebsiella, Enterobacter,
Hafnia, Serratia
Tribe III Proteae : Proteus, Morganella,
Providencia
Tribe IV Yersinieae : Yersinia
Tribe V Erwinieae : Erwinia
Pembagian Famili Enterobacteriaceae
berdasarkan kemampuan meragi laktosa :

Lactose Fermenters

Nonlactose Fermenters
Mac Conkey Media
Pembagian Enterobacteriaceae :

True pathogen Enterobacteriaceae

Opportunistic Enterobacteriaceae
ENTERIC PATHOGEN : Enterobacteriaceae
Gastrointestinal disease

Salmonella

Shigella

Diarrheagenic Escherichia coli

Yersinia
SIFAT BIOKIMIA :

Produksi energi :
Fermentasi karbohidrat (Anaerob / O2 )
Siklus TCA & sistem transpor elektron (O2 cukup)
Meragi glukosa asam (mixed acid pathway), kecuali
Klebsiella (Butanediol fermentative pathway)
Reduksi nitrat nitrit
PERBENIHAN :

Media nondiferensial / non selektif : tidak dapat


dibedakan

Koloni : moist, smooth rough, gray

Hemolisa - / + (tipe )

Media diferensial & selektif


MEDIA UNTUK ISOLASI KUMAN ENTERIK

Media diferensial :
Mac Conkey agar
Eosin Methylene Blue agar (EMB)
Desoxycholate agar

Media selektif :
Salmonella Shigella agar
Desoxycholate citrate agar
Hektoen enteric agar
Xylose-lysine desoxycholate agar
Salmonella Shigella Agar (SS)
DAYA TAHAN :

Rusak :
Panas
Germisida & Desinfektan [ ]
Bakterisidal :
Phenol
Formaldehid
-glutaraldehid
Halogen
Bakteriostatik :
Quaternary ammonium compound
PENYAKIT

UTI

Wound infections

Pneumonia

Meningitis

Sepsis

Beberapa gangguan gastrointestinal


Identifikasi
Media TSI (Triple Sugar Iron) agar

Uji biokimia :
Indole
Methyl red
Voges-Prouskauer reaction
Urea
Simmons Citrate agar
Identification

Triple sugar iron agar

0.1% glucose
1% lactose
1% sucrose
Ferrous sulfate
pH indicator: phenol red
Triple Sugar Iron
Agar
TSI Media
Indol Test
Indole Test
Methyl Red/Voges Proskauer (MR/VP)
Media dan Reagen
Glucose Broth
Methyl Red indicator for acid
Voges Proskauer reagentsA: 5% Alpha-Naphthol, &
ethanol, B: Potassium Hydroxide, & Deionized Water.
Cara inokulasi kuman pada 2 media cair
glukosa. Inkubasi 48 jam. Tetes masing2 MR dan VP
MRtests for acid end products from glucose
fermentation.
VPtests for acetoin production from glucose
fermentation.
MR/VP continued
Reading Results:
MR a + result is red (indicating pH below 6) and a result is yellow
(indicating no acid production)
VPA + result is red after VP reagents are added (indicating the presence of
acetoin) and a result is no color change.

Methyl Red: left and right + VP: left + and right


MR Test
VP test
Motility
Deteksi pergerakan bakteri

Media semisolid

Indikator Tetrazolium (TTC)

Tusuk media semisolid dengan needle


Motility

From left to right:


+ +
Motility Medium
Citrate

Cara inokulasi kuman pada slant


Media and Reagents Used: Simmons Citrate Agar
contains sodium citrate (carbon source), ammonium
ion (nitrogen source), & pH indicatorbromthymol
blue.
Hasil
Bila kuman dapat memetabolisme sitrat, maka
media akan menjadi biru
Citrate

Left positive and right negative.


Simmons Citrate Agar Media
Urease Test

H2O
Urea CO2 + NH3 NH4+ + OH-
Enterotube
Microbact
Enterobacteriaceae
E.coli
Enterobacteriaceae

Klebsiella pneumoniae
Enterobacteriaceae

Serratia marcescens
Salmonella
Enterobacteriaceae

Proteus mirabilis

Profil Biokimia

Koloni
Sekian