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What is Lipase? Where is it found? Function Clinical Significance & Profile Lipase & Amylase tests Infinite Lipase Infinite Lipase - Features
What is LIPASE?
A lipase is a water-soluble enzyme that catalyzes the hydrolysis of ester bonds in water–insoluble, lipid substrates. water–insoluble Lipases thus comprise a subclass of the esterases.
Where is it found?
Lipases are present throughout our body. Most of the lipase is manufactured in the pancreas, although some of it is also secreted in the saliva.
Where is it found?
Pancreas produces about litre and a half of digestive juices per day e.g. protease, amylase, lipase, cellulase & lactase. Lipase is the enzyme, which is produced to digest fats.
Sources of Lipase…
The different types of lipases found in our body include –
– Pancreatic lipase – secreted by pancreas – Hepatic lipase – present in Liver – Lipoprotein Lipase/Endothelial lipase – present in the endothelial cells – Lysosomal lipase – present intracellularly – Gastric lipase – secreted in the stomach
Lipases perform essential roles in the:• Digestion • Transport & • Processing of dietary lipids in mostif not all- living organisms. (e.g. triglycerides, fats, oils)
Hydrolysis of triglyceride into monoglyceride and free fatty acids is accomplished predominantly by pancreatic lipase. The activity of this enzyme is to clip the fatty acids at positions 1 and 3 of the triglyceride, leaving two free fatty acids and a 2-monoglyceride.
Hydrolysis of triglyceride into monoglyceride and free fatty acids
1 2 3
Elevated levels of lipase in blood may indicate a problem related to pancreas. When the pancreas are inflamed, increased blood levels of the pancreatic enzymes, amylase and lipase will result. The normal lipase level is 13-60 U/L. Normal values may vary from laboratory to laboratory.
• Causes of hyperlipasemia (high lipase levels) may include:
– Pancreatitis - also known as inflammation of the pancreas, can cause amylase and lipase levels to be increased up to 3 times normal. Both values should be increased, in order to carry the diagnosis of pancreatitis. – Lipase may be increased in tumors of the pancreas, or stomach. – Gall bladder infection - Inflammation of the gall bladder (cholecystitis), may cause increased lipase levels. – Kidney failure can cause hyperlipasemia.
Related Test: Amylase
Lipase & Amylase tests
Amylase & Lipase tests are used to monitor the diagnosis and treatment of acute pancreatitis and to differentiate pancreatitis from other abdominal disorders Blood amylase levels are sensitive for pancreatic disorders but are not specific. An elevated amylase may indicate a problem but non-pancreatic disorders can cause increased amylase levels.
Lipase & Amylase tests
Lipase levels are usually increased in pancreatic disorders and are more specific than amylase for diseases of the pancreas. Lipase levels remain in the blood for a longer duration than amylase. Evaluating the results of the two tests together help to diagnose or rule out pancreatitis and other conditions.
water-soluble What is Lipase? enzyme Pancreas Where is it found?
Digestion of dietary Function – Lipids Pancreatic Clinical Significance & Profile – disorders Lipase is more Lipase & Amylase tests – specific & remains longer in serum
1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6methylresorufin) ester Lipase/Colip ase 1,2-o-dilauryl-rac-glycero + glutaric acid-(6methylresorufin) ester
• Simple Assay Procedure:
R1: 800 μl + Sample: 10 μl
Mix & incubate for 5 min at 37°C Mix & aspirate
R2: 200 μl
120 seconds 60 seconds
A 1 A 2
60 seconds A Measure at 578 3 nm.
• Colorimetric method • Increasing Kinetic reaction • Wavelength – 578 nm. • Reaction temperature – 37°C • Zero setting – Reagent Blank
• Delay time – 120 seconds • Interval – 60 seconds • No. of readings – 3 • R1 & R2 volume – 800 μl & 200 μl • Linearity – 300 IU/L • Calculation: Lipase (IU/L) = Factor x ΔAbs./min of Sample
Infinite Lipase - Features
• Ready-to-use, two liquid reagent system • Colorimetric detection of Lipase • High Linearity of 300 IU/l • Free Calibrator provided with the kit • Pack-Size: 25 ml
(R1: 2 x 10 ml; R2: 2 x 2.5 ml; )