Wayne Materi. PhD.

National Institute for Nanotechnology
A Bold Assertion
Mature nanotechnology will have many oI the characteristics,
challenges and problems oI living organisms.
arly Nanotechnology
Passive materials constant physico-chemical properties
Mature Nanotechnology
Active materials Variable physico-chemical properties
unctional Nanobots
Advantages and disadvantages oI inorganic nanobots
ell-understood component behaviour
ease of programming
ease of external control
unlimited chemistry (ith enough energy)
Advantages:
difficult and expensive to make
not self-reproducing
difficulty of communicating ith organic systems
must carry on (limited) payload
Disadvantages:
Advantages and disadvantages oI organic nanobots
easy to make using genetic engineering
self-reproducing (cheap)
easily communicate ith other organic systems
protein factories manufacture payload
Advantages:
poorly understood component behaviour (proteins)
hard to program
limited external control mechanisms
limited to CHON chemistry and needs ater
Disadvantages:
Atherosclerosis
apabilities oI a plaque Iighting nanobot
nanobots are introduced into patient`s bloodstream
move toards atherosclerotic plaque
bind in the region
make and secrete a plaque-dissolving protein ( ApoA-I(M) )
exhibit behavioural dependency
omponents to modiIy/add
adhesins
chemotaxis ApoA-I(M)
production
& secretion
ModiIying tactic response
alke and Hazelbauer, 2001
%rivedi and Spudich, 2003
ooger et al., 2003
omponents to modiIy/add
adhesins
chemotaxis ApoA-I(M)
production
& secretion
ModiIying binding behaviour oI adhesins
imH binding sites
receptor
A Iew challenges
tactic response can`t interact ith external protein
tactic response does not activate transcription
connecting regulation
adhesins
chemotaxis ApoA-I(M)
production
& secretion
verall goals oI the proiect
demonstrate complex programmable behaviour in .4
develop some novel sensory systems
develop some autonomous regulation mechanisms
explore novel exogenous regulation mechanisms
develop techniques for routine construction of genetic netorks
hallenges
Protein design
Identify candidate mechanisms
Rationale design + Directed evolution
External control (Left-handed sugars / Right-handed amino acids)
Status communication (Secreted peptides/small molecules / GFP)
Internal logic
Integrate engineered genes into chromosome
Limit groth
Limit immune response
mmediate goals
Chromosomal Integration
Directed evolution ith feedback
Acknowledgements
avid Wishart
Shannon ubitz
Valentyna Semenchenko
Members oI .4community

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