ODV TEST

S.Kannan
Senior Agricultural Officer
Seed Testing Laboratory ,Thiruvarur
Benefits from the
breeding can only be
transferred to the
farmer if good quality
seed is released
Farmer’s expectations can
only be achieved if the seed
is true to the selected variety
What do we want from our seeds?

Uniform,
successful
establishment
WHAT WE DO NOT WANT TO SEE

Weedy crops
 Disease
d crops

Crops
Uneven
maturity
 Availability of quality seeds of improved cultivars is considered

crucial for realizing productivity . The quality of seed alone is

known to account for at least 10-15% increase in the productivity

(ICAR 1993).

• The good quality seed should have the following characters:

Genetic purity, and uniformity and should conform to the

standards of the particular cultivar.
Disease free, viable seeds.
Free from admixtures of other crop seeds, weeds and inert
matter.
Acceptable uniformity with respect to size, shape and color .
 SEED QUALITY

SEED QUALITY IS THE

POSSESSION OF SEED WITH
REQUIRED GENETIC AND
PHYSICAL PURITY THAT IS
ACCOMPANIED WITH
PHYSIOLOGICAL SOUNDNESS
AND HEALTH STATUS.
 1. Physical Quality:

• It is the cleanliness of seed from other seeds, debris,

inert matter, diseased seed and insect damaged seed.

• The seed with physical quality should have

uniform size, weight, and colour and should be
free from stones, debris, and dust, leafs, twigs,
stems, flowers, fruit well without other crop
seeds and inert material.
 Genetic purity:

It is the true to type nature of the seed.

i.e., the seedling / plant / tree from the seed
should resemble its mother in all aspects.
This quality character is important for
achieving the desired goal of raising the
crop either yield or for resistance or for
desired quality factors.
 Physiological Quality:

• It is the actual expression of seed in

further generation / multiplication.
Physiological quality characters of
seed comprises of seed germination
and seed vigour.
 Seed Quality
 Required Variety -ODV Test

 Freedom from weeds,inert material and OCS

-Physical Purity Test

 Good Plant Establishment

-Germination Test

 Freedom from diseases

-Seed health Test
 Determination of ODV
To Determine the extent of Seeds of other distinguishable varieties
 
Determination of other distinguishable Characters shall be done only on the
bass of readily apparent differences in the stable morphological
characteristics of the seeds.
 
The Morphological Characters are examined with the aid of a suitable
magnification
 
The Colour Characteristics are examined under full day light or light of the
limited Spectrum
 
The availability of authentic Sample (Breeder Seed) for comparison is must
for this ODV determination
 Morphological Characters

Size and shape of the grain


Base of Lemma

Wrinkling of Lemma and Palea

Deviation of Lateral dorsal nerves

Rachilla hairs

Shape and Hairiness of lodicules

Seed coat bloom

Hilum colour (Dicot)

Seed Colour

 Methodology
• Place the Seeds on Purity Working board
• Examine each Seeds with the aid of Magnifying
lens
• Separate the seeds differ in Morphological
Characteristics
• Count the Number
• Express ODV by No/Kg for all Crops except Rice
• For Rice ODV is expressed by Weight/ Weight as
Percentage
 ODV Calculation in Rice

• Whole Submitted Sample

• For Paddy – 400gram
• Spread the Sample on white surface
• Use Magnification Board / Lense
• Separate the seed differ from the variety
• Count the Number of other Varietal Seeds
• Put the differed Varietal Characteristics Seed
in Small poly beg & Mention the No in Cover
 ODV CALCULATION
• ODV is expressed in – Weight/Weight basis in
Percentage (%)-Paddy

ODV is expressed in –NO/Weight

-All crops
 ODV FORMULA FOR PADDY
No. Of Test Wt.
other of
ODV varietal X X 100
Seed
Paddy grams X
X
X
Sample Size X 1000
X
X
 Red Rice in ODV
A grain is considered to be Red if more than 25% of its Surface area is Red
coloured or streaked
Method to calculate Red rice

Take a beak cup size of seed – 2000Nos

Rub the seed with mortar/ Paddy De Huler
Count the No. Of Red Grains
 Express – in Percentage

= X 100
If Red Rice - 1 2000

= 0.05%
If 1 Red – 0.05%
2 Red – 0.05 X2=0.1%
3 Red – 0.05 X3=0.15%
 ODV LIMITS
CROP F C

Paddy 0.05% 0.2%

Sorghum 10 20
Maize 5 10
Redgram 10 20
Blackgram 10 20
Greengram 10 20
Cowpea 5 10
Chickpea 5 10
peas 5 10
Horsgram 5 10
Gingly 10 20
castor 5 10
Soybean 10 20
DISTINGUISHABLE
PROPERTIES OF
CULTIVARS
Distinguishable properties of cultivars

PADDY
PADDY Grain size and Shape
Scale Classification Length
1 Extra Long more than 7.5 mm

3 Long (6.0 mm to 7.5mm)

5 Medium (5.51mm to6.6 mm)

7 Short (5.5mm or less)

Scale Shape Ratio

1 Slender Over 3
3 Medium 2.1 to 3.0
5 Bold 1.1 to 2.0
9 Round Less than 1.1
 Size &Shape
1 Extra Long Slender Basumathi, ADT 41

2 Long Slender ADT42,ADT(R) 46 ADT(R)

48,TKM11,MDU4,PMK4,IR 50,TRY 2
3 Medium Slender CO43,White Ponni, Co 47,ADTY39,ADT43,
ADT(R ) 45 Co 50,Co 51,ADT50,TRY 1 ADT38

4 Short Slender ASD 19,ASD 20

5 Long Bold PMK(R )3,ASD4,ASD 13,RMD(R )1
6 Medium Bold PMK 2,PS( R) 4,TRY 3
7 Short Bold ASD7,ASD8,ASD9,TKM5,ADT26,ASD16,ADT37
CR1009,TPS2,TKM 9,CR 1009 Sub -1

8 Short round Co 33 (Karuna)

9 Fine Co 41 ,ASD 15
Based on seed
colour
 Based on Seed Colour
S.N Seed Colour Variety
o
1 Golden ADT 40,ADT37,CO
43,ASD 19,White ponni,
ASD 20,MDU 5

2 Yellow IR 50,ASd 16,ADT 38,IR

20,CORH 2,CR 1009

3 Brown ADT 39,CO 46,PY 1,PY 6

4 Straw Colour ADTRH 1,TKM 9,ADT 43

BASED ON TEGMEN COLOUR
 PeriCorp &Tegmen Colour
S. Pericarp & Tegmen Variety
No Colour
1 white ADT 37,ADT 38,ADT 43,ADT
(R)46,CO43,
CO 50,IWP,Bhavani
2 Greyish Brown CO 48,CO 49
3 Variegated Brown IR 20,CR 1009,IR50
4 Yellow PY 1,ADT 39
5 Red TKM 9
6 Golden
7 Reddish Purple
8 Full Purple(almost
Black)
 Beak value
S. Beak value Variety
N
o
1 Curved IR 50,ASD 20,ADTRH
1,TKM 9,ASD 20,CO 45
2 Slightly CO 6,CORH 2,CO 43,ADT
curved 39,ASD 16,ADT38
ADT40 ,MDU 5
3 Straight ADT 43,IR 20,White Ponni,
CR 1009
Virtuous character
 Vitreous Character
S.No Vitreous Variety
character
1 Side is IR 20,White Ponni, CR 1009,ADT 37
Extended
2 Side is small TKM 9CORH 2,ADTRH 1,CO 43

3 Side is ADT 38,ADT 39,CO 45,CO 46,ASD

absent 20,MDU 5
ADT 43,IR 50,ADT 40
 1000 Grain weight(g)
S. I000 grain Variety
N weight(g)
o
1 Very Low(<15 g) White ponni,ADT 39,ADT
43,ASD 19
2 Low (15 to 20)g Co 43,IR 20,ADT 38,IR 50
3 Medium(21-25)g CR 1009,TKM 9,ADT 40,ASD
16,CO 46
4 High(26-30 g) CO 45,
5 Very high(>30 g)
 Sorghum
 Grain weight

 Grain shape

 Grain colour
Grain shape dorsal view
Grain shape- profile value
Grain shape- profile value
 Grain size
Very small Small(16-25 g) Medium (26- Large(>36g)
35)g
APK 1 K9 BSR 1

CO 26 TNAU Co 30 K 12

CO (S) 28 K10 Paiyur 2

Paiyur 1 CO H 4

CO 25
 Varietal Property- Sorghum
S. Seed Variety
No Colour

1 Yellow Co 27
2 White Co (FS)28,K11
3 Pearly K 8, K 9,CO 25CSH
White 9,CSH 5,CSH 9,Paiyur 1
4 Red Colour Paiyur 2
 Grain shape sorghum
S. Grain shape Variety
No

1 Narrow K12
Elliptic

2 Elliptic K-Ttall, CSH 9

3 circular CSH 5,
 Varietal Property -Cumbu
S. Seed Variety
N Colour
o
1 Slate Co 7
Colour
2 Yellowish Co (Cu) 9
Grey TNAU cumbu Hybrid
Co 9
3 Amber Co (Cu) 9
Colour
 Maize
Variety Properity
CO 1 Flint type bold kernal with deep
orange colour
Co BC 1 Dent type corn with yellow colour
CO H 2 Flint type with orange yellow
CO H 3
CO (M ) 4 Flint type with yellow in colour
TNAU hybrid Semi dent type with orange yellow in
CO 6 colour
 Ragi
Variety Properity
CO 7, Brownish seed
CO 12
Paiyur 2
Co (Ra)14
CO 13 Light Brown
 Varietal Property -Samai
S. Seed Variety
N Colour
o
1 Greyish Co (samai)4
yellow
2 Light CO 3
grey
brown
3 Brown Paiyur 1 ,Paiyur 2
 Seed colour
Green Greenish Brown Black Mottled
Brown
 Seed colour
Green Shekara 1
Black ADT 4,K1,Vamban 2,vamban
8
Dull Block ADT 5,APK 1,Vamban
1,Vamban 3,TMV 1
VAM (Bg)5,Vamban 7
Brown MDU 1

Prominent Hilum Vamban 1

Slightly hilum MDU 1
 Seed lusture
Shiny Dull
 Seed shape
Globose Oval Drum
 Seed size
Small Medium Large
(1000 seed (1000 seed (1000 seed
wt<30 g) wt30-50 g) wt>50 g)
 Seed size
Size Variety
Small
(1000 seed wt<30 g)
Medium ADT4,APK
(1000 seed wt30-50 g) 1ADT5Vamban
1,Vamban 2,vamnan
3,VBN(Bg) 4,TNAU BG
VBN 6,Vamban 8
Large Co 6,K1,TMV 1,K1,TMV
(1000 seed wt>50 g) 1MDU 1
GREEN GRAM
 Seed colour
Seed colour variety
Dull reen ADT 3,CO 4,CO 5,CO 8

K 1paiyur 1,vamban 1

VBN (Gg)3
Shiny Green CO 6,CO 7,KM 2

VBN(Gg) 2,VRM (Gg) 1

Brown
 Seed size
Seed colour variety
small ADT 3,CO 4,CO 5
Medium Bold CO 6,CO 7,KM 2
Vamban 1,VBN(Gg)2
VRM(Gg)1

Bold Co 4,CO 8,VBN(Gg)3

 Seed shape

Seed colour variety

Oblong CO 4

Drum shape CO 8
RED GRAM
 Varietal Property -RED gram
Seed Colour Variety
Brown seeded APK 1VAMBAN 1

Purple Seeded BSR 1

Light Brown
CO 4CO PH 1

Reddish Brown CO 5,Vamban 2

CO( Rg )7 ,VBN(Rg )3
Tan Brown CO OH 2
COW
PEA
 Cow pea seed shape
size and Shape Variety
Medium size Kidney CO 2
shape
Largesize Rectangular Co 4
Smallsize Reniform Co 6
shape
Medium square shape CO (Cu)7
 Cowpea -Seed colour
Colour Variety
Reddish brown Co 2CO 4
Grayish Brown CO 4,CO 3
Cream colour CO 6 ,CO (cp)7
White Vamban1
Brick red Paiyur 1
Ivory white hilum with Vamban 2
Benal Gram
Beak Size
Seed Colour
Seed size
 Benal gram variety
Variety Character
Co 3 Brown in colour with bold in size
and less or no wrinkles

CO 4 Dark Brown in colour with bold

in size and less or no wrinkles
hilum is prominent
GROUND NUT
 Morphological Character
Pod Seed
Pod Beak Seed Size
Pod Constriction Seed Shape
Pod reticulation Seed Colour
Pod ridge Seed Colouring
pattern
Number of seeds per
pod
CHARACTER:POD BEAK
Character: Pod Constriction
Character: Pod reticulation
Character :Pod ridge
Character: No of Seeds per pod
Character; Seed Size
Character :Seed Shape
Character :Seed Colour
Character:Colour pattern
 Pod beak
Absent VRI(Gn)6
slight ALR 2,CO(Gn) 4,CO 7 TMV 7 TMV
(Gn)13,VRI 4
Moderate CO 2,CO 6,VRI 2,VRI 3,VRI(Gn)5
Prominent ALR 1, CO(Gn) 5,TMV 2,
TMV 10,VRI 1

Very TMV 12
Prominent
 POD Constriction
None VRI 4
slight CO (Gn) 4, CO (Gn) 5,CO 7,TMV 10
TMV 12,TMV (Gn)13
Moderate ALR 3,BSR G 1,CO 6,TMV 7,VRI 2
VRI 3
Deep CO 2,TMV 2,VRI 1,VRI (Gn)5

Very Deep
 Pod Reticulation
None
Slight CO (Gn)4,TMV 10,TMV 12
Moderate ALR 2,ALR 3,BSR G1, CO
(Gn)5,CO 6 CO 7
TMV( Gn) 13,VRI 3
Prominent ALR 1,CO 2,TMV 2, TMV 7,VRI
1,VRI 2,VRI (Gn) 5
 Seed colour
Purple VARIETY
Red VRI (Gn)5,TMV (Gn)13,
CO (Gn)5,ALR 1
Tan VRI 4,CO7,CO 3,ALR 3
Rose VRI (Gn)7,TMV 12,TMV 7,
CO (Gn)4,CO 2 ,BSR G 1
Dark Tan
 Seed size
Size 1000 grain Varieties
weight(g)
Low < 360 TMV 2,TMV 7VRI 3,VRI(Gn) 6
Mediu 360 to 500 ALR 3,CO (Gn)5,CO 6TMV
m 10TMV 12.TMV(Gn)13 VRI
1 ,VRI 2,VRI (Gn)5,VRI (Gn)7
High >500 BSR G 1,CO 2,CO 3,CO(Gn)4
 Seed shape

Spheroidal CO 2,VRI (Gn)5

Cylindrical ALR 3
Fusi TMV 2,TMV 7.TMV
form(round 12
)
Gingelly
Ginelly
 Seed colour
White SVPR 1
Light TMV 4,TMV 5
Brown
Dark TMV 6 ,TMV 7TMV (SV)7VRI
Brown (Sv)1,VRI (SV)2
Black CO 1TMV 3
Soya bean
 Soya bean varieties
Variety Seed Characters
CO 1 Medium Size,Creamy colour
weigth 125 g
CO Soya 2 Cream to Yellow Colour,
Ovate Shape,medium in size
hilum is dark brown
(weight 130 to 10 g)
Co( soy) 3 Creamy yellow , Brown hilum
 Castor
VARIET CHARACTERS
Y
TMV 3 Red brown with over lay pattern of
dark red colour
TMV 5 Deep chocolate in colour
TMV 6 Medium bold ,Light chocolate in
colour with conspicuous mottling
YRCH 1 Mottled chocolate in colour
 Amaranthus
variety character
CO 1 Very small seed black in colour
CO 2 Black colour compressed with fleshy
endosperm
Co 3 Medium size seed with black colour
Co 5
PLR 1
 ADT 37
SHORT BOLD
Test wt:23.4(g)
L.B.T(mm):5x2.8X1.88
 ASD16
SHORT BOLD
Test wt:24.2(g)
L.B.T(mm):7.86x3.02X1.9
6
 Savithri
SHORT BOLD
Test wt:23.5(g)
L.B.T(mm):6.9x3.1X2.1
s

SHORT SLENDER PADDY VARIETIES

 ASD 19
SHORT Slender
Test wt:18.39(g)
L.B.T(mm):8.28x2.32X1.7
2
MEDIUM SLENDER
VARIETIES
 IR 20
Medium Slender
Test wt:19.0(g)
L.B.T(mm):
 CO ( R) 48
Medium Slender
Test wt: 18(g)
L.B.T(mm):
5.3X1.97X1.7
 CO 43
Medium Slender
Test wt: 20(g)
L.B.T(mm): 8.1X2.3X1.8
 CO 47
Medium Slender
Test wt: 30.6(g)
L.B.T(mm): 7.7X2.3X1.7
 CO ( R) 49
Medium Slender
Test wt: 15.9(g)
L.B.T(mm):
5.6X2.01X2.79
 CO 50
Medium Slender
Test wt: 20.5(g)
L.B.T(mm):
6.1X2.1X1.9
 CO 51
Medium Slender
Test wt: (g)
L.B.T(mm):
 IWP
Medium Slender
Test wt: 16.4 (g)
L.B.T(mm):
8.0X3.0X2.0
 IWP
Medium Slender
Test wt: 16.4 (g)
L.B.T(mm):
8.0X3.0X2.0
 ADT 38
Medium Slender
Test wt: 21 (g)
L.B.T(mm):
6.9X2.4X2.0
 ADT 39
Medium Slender
Test wt: 18 (g)
L.B.T(mm):
7.6X2.3X1.9
 ADT 43
Medium Slender
Test wt: 15.5 (g)
L.B.T(mm):
5.6X1.94X1.63
 ADT(R) 45
Medium Slender
Test wt: 17.5 (g)
L.B.T(mm): 8.0 X 2.16 X
1.97
ADT 49
ADT 50
 ADT 50
Medium Slender
Test wt: 15.9 (g)
L.B.T(mm):
7.24X3.5X1.65
LONG SLENDER
VARIETY
 IR 50
Long Slender
Test wt: 20.5 (g)
L.B.T(mm):
8.9X2.3X1.8
 Bhavani
Long Slender
Test wt: 21.5 (g)
L.B.T(mm):
9.6X2.03X1.5
 ADT (R ) 46
Long Slender
Test wt: 23.8 (g)
L.B.T(mm):
9.58X2.46X1.95
 Anna 4
Long Slender
Test wt: 25.7 (g)
L.B.T(mm):
EXTRA LONG SLENDER :BASUMATHI TYPES
Black gram VBN(BG)4

Grain Colour
-Black
Test Wt: 4.8 (g)
 Black gram CO 1
Grain Colour
-Black
Test Wt: (g)
 Green gram CO (GG) 7

Grain Co(lour -Green

Test Wt: (g)
 Green gram VBN (GG) 2

Grain Colour
-ShinyGreen
Test Wt: 3.6 -3.9 (g)
 Green gram CO 6

Grain Colour -
Test Wt: (g)
 Cow pea CO (CP) 7
Grain Colour -Browinish
white square shape
Test Wt: 12-14 (g)
 Cow pea VBN (CP) 2
Grain Colour –Ivory white grain
hilum with tan rings surrounded by
brown base
Test Wt: 13-15 (g)
 Cow pea Co 87
Grain Colour
Test Wt: (g)
 Gingelly SVPR 1
seedColour :White
Test Wt: (g)