Northern Blotting

Mata Kuliah: Biologi Molekuler

Dosen Pengampu: Dr. Yustinus Ulung Anggraito, M.Si.

disusun oleh:
Arista Novihana Pratiwi 0402518007
Northern Blotting is a technique
used for the study of gene
expression

It is done by detection of
particular RNA (isolated mRNA)
 GENERAL PROCEDURE FOR
BLOTTING
1. Homogenize the sample.
2. Separation of the molecule of interest by
an electrophoresis membrane.
3. Transferring the molecules to a nitro
cellulosic membrane/ nylon membrane.
4. Hybridization or identification of the
molecule.
Prosedur Northern Blotting
Langkah transfer gel dari analisis northern blotting
Electroblotting dengan unit transfer semidry
Sistem untuk mendeteksi dan memverifikasi
mirtron mamalia
Pengaruh jenis bakteri inang pada hasil RNA AluY
 Total RNA ragi
dalam fase
pertumbuhan
stasioner dan
pertengahan log
diperlakukan atau
tidak diobati
dengan
Terminator
 Fucoidan menekan
replikasi HBV

Northern blotting dilakukan untuk mengukur kadar

RNA HBV intraseluler setelah pengobatan fucoidan
pada sel tikus.
Video Northern Blotting
 Daftar Pustaka
Bikard David, et al. 2013. Programmable Repression and activation of Bacterial Gane Expression Using an Engineered CRISPS-Cas
System. Nucleic Acids Research. https://doi.org/10.1093/nar/gkt520.
Brauburger Kristina, Cressey Tessa, dkk. 2017. Nonradioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNA.
Ebolaviruses: Methods and Protocols, Methods in Molecular Biology. Spinger Science. Chapter 11, pp (143-159).
https://doi.org/0.1007/978-1-4939-7116-9_11.
Fleischamann Jacob, Rocha A. Miguel. 2018. Nutrient Depletion and TOR Inhibition Induce 18S and 25S Ribosomal RNAs Resistant to
a 5’-phospate-dependent Exonuclease in Candida albicans and Other Yeasts. BMC Molecular Biology.
https://doi.org/10.1186/s12867-018-0102-y.
Inc Elsevier. 2015. Electrophoresis-Blotting Techniques. University of Oklahoma Health Sciences Center. USA.
http://dx.doi.org/10.1016/B978-0-12-409547-2.11157-6.
Josefsen Knut, Nielsen Henrik. 2011. Northern Blotting Analysis. RNA, Methods in Molecular Biology. Spinger Science. Chapter 7, pp
(87-105). https://doi.org/10.1007/978-1-59745-248-9_7.
Li Huifang, dkk. 2017. Fucoidan From Fucus Vesiculosus Suppresses Hepatitis B Virus Replication by Enhancing Extracellular Signal-
Regulated Kinase Activation. BMC Virology Journal. https://doi.org/10.1186/s12985-017-0848-8.
Liu Chao, dkk. 2017. The Expression and Construction of Engineering Escherichia coli Producing Humanized AluY RNAs. BMC
MicrobialCellFactories. https://doi.org/10.1186/s12934-017-0800-z.
Negritto C. Maria, Manthey M. Glenn. 2016. Overview of Blotting. Current Protocols Essential Laboratory Techniques.
https://doi.org/10.1002/cpet.5.
Tang Wei, Michael. 2018. Oligopeptide Polyarginine-mediated delivery of short interfering RNA to cell for gane silencing. MedDocs
Publishers LLC. Annals of Biotechnology Journal.
Zia Farid Muhammad, Flynt S.Alex. 2018. Detection and Verification of Mammalian Mirtrons by Northern Blotting. miRNA Biogenesis:
Methods and Protocols, Methods in Molecular Biology. Spinger Science. Chapter 12, pp (210-219).
https://doi.org/10.1007/978-1-4939-8624-8_16.
TERIMAKASIH