CELL CULTURE IS ONE OF THE MAJOR TOOLS

USED IN CELLULAR AND MOLECULAR BIOLOGY

providing excellent model systems for:

studying the normal physiology and biochemistry of cells (e.g.,
metabolic studies, aging)
 DRUG SCREENING AND DEVELOPMENT
(EFFECTS OF DRUGS AND TOXIC COMPOUNDS ON THE
CELLS)
ANCER RESEARCH (IN VITRO)
LARGE SCALE
MANUFACTURING OF
BIOLOGICAL COMPOUNDS
SOME BIOLOGICAL
SCIENTISTS WORK TO
CREATE ARTIFICIAL
SOURCES OF HUMAN
PROTEINS.
 WHAT IS CELL CULTURE?
Cell culture
refers to the removal of cells from an
animal or plant and their subsequent growth
in a favorable artificial environment.
What type of cells can you culture?
Primary culture (stage of the culture after PRIMARY CULTURE
the cells are removed from the tissue)
Cell Line (After the first subculture, the
primary culture becomes known as a cell SUBCULTURE (PASSAGE)
line or subclone)
2 types of cell line: CELL LINE
Finite vs Continuous Cell Line FINITE CONTINUOUS CELL LINE
(transformed/immortal)
AS CELLS IN CULTURE GROW AND DIVIDE, THEY
USE UP NUTRIENTS AND OCCUPY ENTIRE SPACE
Using aseptic technique:
1) Check Confluency
2) Remove old media
3) Wash with PBS
4) Incubate with Trypsin/ EDTA
5) Give new media

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CELLS IN CULTURE CAN BE DIVIDED IN TO THREE BASIC
CATEGORIES BASED ON THEIR SHAPE AND
APPEARANCE

bipolar or multipolar,
have elongated shapes,
and grow attached to a
substrate
EPITHELIAL-LIKE CELLS

polygonal in shape with

more regular
dimensions, and grow
attached to a substrate
in discrete patches.
LYMPHOBLAST-LIKE CELLS

spherical in shape
and usually grown
in suspension
without attaching to
a surface.
BASIC EQUIPMENT
https://www.researchgate.net/publication/257531532_The_Use_of_Porous_Scaffol
d_as_a_Tumor_Model/figures?lo=1&utm_source=google&utm_medium=organic
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