BAKTERI

DALAM BERBAGAI PEWARNAAN

Oleh : ASEP DERMAWAN SKM., M.Kes

 BENTUK : Bulat
WARNA : Ungu
SUSUNAN : berderet, spt. rantai
Streptococcus sp SIFAT : Gram (+)
CONTOH : Streptococcus pyogenes (β)
Streptococcus viridans (α)
Streptococcus mitis (gama)
Staphylococcus sp

BENTUK : Bulat
WARNA : Ungu
SUSUNAN : Satu-satu, dua-dua, bergerobol
SIFAT : Gram (+)
CONTOH : Staphylococcus aureus
Staphylococcus epidermidis
Staphylococcus saprofiticus
Pneumococcus

BENTUK : Bulat
WARNA : Ungu
SUSUNAN : dua-dua, spt. lancet
SIFAT : Gram (+)
CONTOH : Streptococcus pneumoniae
Diplococcus pneumoniae
pneumococcus
 Capsules
slide 6 Unspecified species Ba 016a (~1000x)
This slide has been negatively stained (with congo red or eosin?) to show the presence of
the mucopolysaccharide "slime" capsule.
(Capsules are large & regular in Klebsiella, less so in Enterobacter & uncommon in the other species)

BENTUK : Batang
METODE WARNA : Merah Kapsul : Bening
BURRY-GINS SUSUNAN : Tersebar, satu-satu
SIFAT : Kapsul (+)
CONTOH : Klebseilla pneumoniae
 Neisseria sp

BENTUK : Bulat
WARNA : Merah
SUSUNAN : Dua-dua, spt. Biji kopi
SIFAT : Gram (-)
CONTOH : Neisseria gonorrhoae
Neisseria meningitidis
Neisseria pharingis
BENTUK : Batang
WARNA : Ungu
SUSUNAN : Satu-satu, dua-dua, spt. rantai
SIFAT : Gram (+)
CONTOH : Bacillus subtilis, cereus, anthraxis
Corynebacterium diphteriae
 Bacillus subtilis
90 W 0533 (~1000x)
Members of the genus Bacillus are Gram positive endospore-forming rods. Species of
Bacillus are ubiquitous in soil, and most, including B. subtilis, are non-pathogenic. B.
subtilis has been a workhorse in Micro labs for more than a century. Bacitracin and
polymyxin were isolated from members of this genus.
BENTUK : Batang
WARNA : Merah
SUSUNAN : Satu-satu, dua-dua, tersebar
SIFAT : Gram (-)
CONTOH : Escherichia coli
Salmonella typhi
Klebsiella pneumoniae
Mycobacterium sp

Pewarnaan BTA BENTUK : Batang halus, spt. filamen

WARNA : Merah
Metode : SUSUNAN : Tersebar
- ZIEHL NEELSEN SIFAT : BTA (+)
CONTOH : Mycobacterium tuberculosis
- KINYOUN GABET Mycobacterium leprae
Mycobacterium tuberculosis
Mycobacterium tuberculosis
Mycobacterium tuberculosis kolonijas uz Lēvenšteina-Jensena
barotnes. (http://textbookofbacteriology.net/tuberculosis.html)
Metode : Albert

BENTUK : Batang halus

WARNA : BATANG: HIJAU GRANULA:uNGU
SUSUNAN : V, L, T, Y, I (Spt. Huruf Cina)
SIFAT : Granula(+)
CONTOH : Corynebacterium diphtheriae
 Mycobacteria
slide 4 Mycobacterium tuberculosis, sputum 90 W 2054 (~1000x)
This acid fast stained slide shows the pleomorphic shapes of the
etiological agent of tuberculosis. Note that acid-fast stains M.
tuberculosis red, and methylene blue is the counterstain.
Metode :
Schaeffer Fulton

BENTUK : Batang
WARNA : Merah SPORA : Hijau Letak : sentral/ subterminal
SUSUNAN : Tersebar
SIFAT : Spora (+)
CONTOH : Bacillus subtilis
Bacillus cereus
Bacillus antrachis
 Clostridium tetani,
Ba 60 (~2000x)
Note that the cells with spores look like lollypops in this etiological agent of tetanus. It
secretes a protienaceous exotoxin which prevents the re-uptake of acetylk choline at the
neuromuscular junction, leading to paralysis by clonic musclular contraction, a
condition known as tetanus.
 Clostridium botulinum,
Ba 45 (~2000x)
These cells bearing spores are only slightly enlarged at the spore containing end in this etiological
agent of botulism, caused by a protienaceous exotoxin which interferes with nerve to muscle
communication, leading to flacid paralysis.
Treponema pallidum >>> Syphilis
 Flagella
Proteus vulgaris Ba 156 (~2000x)
A special stain (pararosaniline) has been used to build up the flagella so that
they can be seen on this peritrichous bacillus
Flagella (Proteus vulgaris )
 Neisseria
Neisseria gonorrhoeae Ba 133 (~1000x)
This is a Gram stained smear of a purulent urethral discharge. Large numbers of
polymorphonucleocytes are present with a few squamous cells. In the cytoplasm of
the PMNs can be seen phagocytosed Gm- diplococci which are indicative of
gonorrheal infection
MORFOLOGI KOLONI
Neisseria sp pada Agar Coklat – Tayer Martin
Escherichia coli in Mac Conkey Agar
•E. coli & most of other
enteric bacteria from
circular, convex, smooth
colonies with distinct
edge

•Some strains of E. coli

produce hemolysis on
blood agar

•Enterobacter : similar
but more mucoid
Klebsiella sp. : large & very mucoid
 The Salmonellae & Shigellae
produce colonies similar to E. coli
but do not ferment lactose
Salmonella paratyphi B/C
Staphylococcus aureus
Staphylococcus aureus on Manitol Salt Agar
Staphylococcus saprophyticus
Staphylococcus epidermidis
Streptococcus pneumoniae (Pneumokokken)
Streptococcus pyogenes
Streptococcus agalactiae
Proteus vulgaris
Proteus mirabilis
Pseudomonas aeruginosa
Staphylococcus aureus on Manitol Salt agar
 BACTERIA ON PREPARED SLIDES
©David B. Fankhauser, Ph.D.,
Professor of Biology and Chemistry
University of Cincinnati Clermont College,
Batavia OH 45103.
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