Important analysis, in that the concentration of
other nutrients is usually expressed on a dry
matter basis Dry matter analysis is the simplest analysis in the lab Moisture content is simply the loss in weight from evaporation of water Problems associated with DM estimation are with 100C are loss of volatiles such as volatile fatty acids and essential oils (menthol, camphor) Air dry Refers to a sample that has been allowed to dry in air, without aid of an oven or other drying device In air of less than 60% relative humidity most air- dry samples will contain about 90% ± 2 % dry matter This procedure is applicable for the determination of dry matter on ground air- dry or partially dried (≥85% dry matter) forages with low volatile acid content This procedure may be used for determination of DM on forage samples or for dry weight determinations of fiber residues following fiber extraction Volatile acids and alcohols may be lost from fermented samples Dry empty container in microwave for 3 min at full power Place sample in oven and microwave at full power for about 3 min. Remove sample and mix. Repeat these steps three times for silage (35% DM) Return sample to microwave at 50% power for 1 minute and re-weigh sample and container Repeat this step until no weight loss occurs during drying interval Do not allow sample to char or burn Samples with higher moisture contents require longer drying times. Increase the number of drying intervals, not the time per drying interval or microwave power level, as these may lead to charred or burned samples Do not place any metal item or aluminum foil into the oven. Sparking or crackling in the oven indicates the presence of metal This procedure is applicable for determining laboratory DM of ground, air-dry or partially dried (90 to 95% DM) forage
Samples must be ground with 1 mm screen
and be 90 to 95% DM Principle The moisture of sample is lost by volatilization caused by heat. The amount of material left after the removal of moisture is the DM Equipment A) Oven 105 C B) Covered aluminum dishes 50 mm diameter with covers C) Desiccator Procedure Keep the weighing dish in drying oven for one hour at 105C 2. After an hour transfer the dish to desiccator and after cooling weight it (A grams). Handle dishes with metal tong Take about 5 grams of sample in the dried dish and note the collective weight if dish and sample (B grams) 4. Keep the dish in drying oven at 105°C for one hour Then reduce the temperature to 65°C until it attains constant weight Cool down the dish in desiccator DM%= Weight of dried sample/Weight of sample before drying x100