EXERCISE 8

Cellular Respiration
August 31, 2010

CAPUNO, Bingle Mariangiola MATEO, Femina Margaret MENDOZA, Edgardo Jr. NUEVAS, Jan Patrice PESIGAN, Jane Meirial SISCAR, Justine Alyssa Group 3 TFCD1 Prof. Sedricke lapuz

Table of Contents

Introduction II. Methodology III. Results and Discussions IV. Conclusion
I.

Select one

Bibliography

Respiration often occurs in plants during winter or at times when photosynthesis is not possible. INTRODUCTION Respiration is the breaking down of stored materials found in the body to yield energy. . It is a property of all living things.I.

For this metabolic process to occur. substances such as oxygen and many other enzymes are necessary that is why the rate of respiration greatly depends on the factors affecting enzyme activation. .The process of respiration breaks down stored material such as glucose into ATP which is a readily usable source of energy.

The study also wants to explain the presence of oxygen as a requirement in aerobic respiration and in seed germination. . Another purpose of the study is to figure out the products of respiration and the enzymes involved in the said production. Also.OBJECTIVES The study aims to show the processes happening in cellular respiration and the different factors that affect the process. the study discusses the reason why oxygen is a factor in root growth.

BACK . the exercise will reveal how certain enzymes are required for the process to take place. Generally. In the process.SIGNIFICANCE OF THE STUDY The study was done in order to find out how oxygen becomes an important factor in cellular respiration and what products can cellular respiration give. this exercise will explain the importance of respiration in our daily lives.

METHODOLOGY A.II. Oxygen as a requirement in aerobic respiration and seed germination .

. Two bags of the seeds were suspended in two wide mouthed bottles ² Bottle A containing 20ml of water and Bottle B containing 20ml of pyrogallate solution. The bottles were tightly covered and were placed in a cool place.Approx. equal amounts of soaked corn/mongo seeds were placed in cheesecloth leaving some space for germination. Results were taken after a week of observation.

Oxygen as a factor in root growth .B.

Bottle A had an aerator while Bottle B had none. Root growth was the observed after a week.Two similar cuttings of mayana were put in separate bottles containing equal amounts of water. .

C. Carbon dioxide as a product of respiration .

. Results were taken after days of observation. by rubber tubing and was tightly sealed. The set-ups were put in a cool place. Second set-up: An amount of 50ml Barium hydroxide mixture was put in one flask connected to another flask containing KOH solution. by rubber tubing and was tightly sealed.First set-up: Soaked corn/mongo seeds were placed in a 125ml flask connected to another flask containing 50ml Barium hydroxide mixture.

D. Oxidases & Peroxidases: Some enzymes involved in metabolism .

D ± The fruit strips were put in distilled water. C ± The fruit strips were boiled in distilled water for 3 min. There were five set-ups. A ± The fruit strips were placed in an open petri plate (no solution).The fruit (apple) was peeled and was cut into 15 strips. B ± The fruit strips were immersed in !% solution of ascorbic acid. and were then set aside. E ± The fruit strips were put in 3% hydrogen peroxide solution. The set-ups were observed after 30 minutes and results were taken. each utilizing 3 strips of the fruit. BACK .

III. RESULTS AND DISCUSSIONS A. Oxygen as a requirement in aerobic respiration and seed germination .

1 Mongo seeds.twenty seeds inside the cheesecloth suspended over 20 ml of water Bottle B. soaked overnight Fig 3.Fig.1.1.twenty seeds inside the cheesecloth suspended over 20 ml of pyrogallol After one week . 3.2 Bottle A.

1.3 Bottle A(water).4 Bottle B (pyrogallol).Sixteen out of twenty seeds germinated (80%) Fig 3.Twelve out of twenty seeds germinated (60%) NEXT .1.Fig 3.

Oxygen as a factor in root growth .B.

2.1 Set-up of experiment SetAfter one week .Fig 3.

3 Mayana cuttings without aerator has less roots NEXT .2. 3.2 Mayana cuttings with aerator has more roots Fig.2.Fig 3.

C. Carbon dioxide as a product of respiration .

3.3.Barium hydroxide solution Set-up 2: Flask 1: Potassium hydroxide solution Flask 2: Barium hydroxide solution After one week .Fig.mongo seeds Flask 2 .1 Set-up 1: Flask 1 .

NEXT .white precipitates were present Fig.Fig.2 Seeds and Barium Hydroxide.3 Potassium hydroxide and Barium hydroxide. 3. 3.3.No visible changes were observed.3.

Oxidases & Peroxidases: Some enzymes involved in metabolism .D.

2 Immersed in 1% ascorbic acid solution No discoloration .4. 3.1 Exposed in open air Discoloration.4.brown Fig. 3.Fig.

4.4 Immersed in distilled water No discoloration .Fig.3 Immersed in distilled water then boiled for 3 minutes No discoloration Fig. 3.4. 3.

4.Fig.brown BACK .5 Immersed in 3% hydrogen peroxide solution Discoloration. 3.

IV. oxygen is and important growth factor thus. . seeds emit carbon dioxide as a product of respiration and oxidases and peroxidases prevent oxidation which causes discoloration of fruits and vegetables. CONCLUSION From the results of the experiments conducted. triggers greater growth of roots. it can therefore be concluded that oxygen is a need for the seeds to germinate and to perform aerobic respiration.

harvard. Massachusetts: Jones and Barlett Publishers.chm.Bibliography Mauseth. M.org http://www.G.bris.scijourals.uk http://adsabs. (2006) Plant Systematics.edu y .D.)..ac. J.. USA: Elsevier Academic Press y Internet Sources http://jeq. LLC y Simpson. (2009) Botany: An Introduction to Plant Biology (4th Ed.

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