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ë ÷tationary phase ë Mobile phase ë Column (support for stationary phase) ë Detection on detecting agent. . ë All chromatographic methods involve modifications in these basic components.CHROMATOGRAPHY Chromatographic method must have essentially.

2  2   ë &&! artition of analytes between mobile phase and stagnant phase inside the pore space + adsorption on the surface of bonded phase. ë !&&") 80% of all separations done on R  C. CN) ë "4&! ) Methanol or Acetonitrile and Water. C8. C5. ë &" ) ydrophobic surfaces of moieties bonded on silica (C18. . henyl.

2  2  ë 80% Octadecylsilica (OD÷. C18) ë 10% Octylsilica (C8) ë 5% Butylsilica (C4) ë 3% henyl ë 2% Cyano (CN) .

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8 Nonanol. k¶ = 3.5 Nonane.3 Octanol. k¶ = 7. k¶ = 4. k¶ = 3. Reversed hase Normal hase Octane.3 . Ñ ÷mall differences in component molecular structure could have a significant effect in their retention. ë Nonspecific (hydrophobic) interactions are at least ten times weaker than polar.

FREEY ÷OUBE IN IN METANO ÷ ARINGY METANO ÷OUBE IN ETANO    6' C2025ClN2O5‡C66O3÷ C2028N2O5. ÷IGTY ÷OUBE IN RO ANO FREEY ÷OUBE +'    WATER.C44O4 .  ANGIOTEN÷IN CONVERTING 5'    CACIUM CANNE BOCKER ENZYME (ACE) INIBITOR ÷IGTY ÷OUBE IN WATER.cRUG PROFILE ''      c             .

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÷onicate for 5 min. .÷TANcARc PREPARATION 35 mg Amlodipine Besylate + 100 mg of Enalapril Maleate in 100ml of Methanol. ÷onicate for 30 min & Dilute to 25ml with Mobile phase. ÷AMPLE PREPARATION Take 5 intact capsule in 100 ml of Mobile phase. take 5 ml & dilute to 25 ml Mobile phase.

50319 .911 3000 y = 8.72064 2000 area 1500 1000 160 1284.23462 0 50 100 150 200 250 300 350 concentration(ng/ul) 240 1892.3731 500 0 200 1612.0241x + 3.9993 100 814.LINEARITY CURVE OF ENALAPRIL MALEATE "&"  (ng/ul) linearity curve of enalapril 40 325.3869 2500 R2 = 0.62964 300 2435.

15222 concentration(ng/ul) 84 934.1651 70 795.53259 area 56 631.LINEARITY CURVE OF AMLOcIPINE BE÷YLATE "&"  (ng/ul) linearity curve of amlodipine besylate 14 157.50269 105 1205.86616 - R 35 398.80298 .

3% 77.2% 0.3% 72. 0. 0.8% 2% 0.4% 2.7% 21.0% 3.0% 7.1% %DEG.1% 91.5% 86.0% 100% 91.6% 0.6% 100.2% 15.9% 79.7% 99.6% 22.3% %DEG.2% 98. cEGRAcATION OF ENALAPRIL MALEATE     '  c E   F c        %A÷÷AY 98.1% 90.7% 90.8% .0% cEGRAcATION OF AMLOcIPINE BE÷YLATE     '  c E   F c        %A÷÷AY 93.0% 0.0% 3.

153 0.5 RECI÷ION 0.67 ROBU÷TNE÷÷ % R÷D = 0.094 % R÷D = 0.459 0.3 0.093 .9984 RANGE 14-105 ng/ul 40-300 ng/ul ACCURACY 100.3 100.6 OD 0.÷UMMARY OF VAIDATION ARAMETER÷   c   c            INEARITY R2 = 0.9981 R2 = 0.53 þ 0.63 þ 0.2242 OQ 0.

CONCLU÷ION ence we can conclude that the developed methods is simple and rapid. . Moreover the methods is quite sensitive to determine microgram quantities of drugs and economic too.