Research By: S. Venkata Mohan, S. Veer Raghavulu, S. Srikanth and P. N.

Sarma* Bioengineering and Environmental Engineering Centre, Indian Institute of Chemical Technology, Hyderabad 500 007, India Presented By: Arunjyoti Banik M.Tech (BT) 1st Yr 2nd Sem

Present statistics .

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20 mg/l. 3 g/l. CoCl2.25 g/l. CuCl2. y Dual Chambered MFC. 10 mg/l. CaCl2. 0. MnCl2. 10 mg/l. 4 mg/l.20 g/l. ZnCl2. NH4Cl. H2PO4. K2HPO4. 10 mg/l. MgCl2.5 g/l.20 g/l.Materials Used y Anaerobic Wastewater further enriched with glucose. 0. 0. 0. 5cm X 5cm X 1cm Nafion 117 .

Schematic diagram of the MFC 50Ÿ Glucose Aerated Po4 Buffer CO2 Mechanism of Current Generation .

Operation y Air was continuously sparged maintaining dissolved oxygen (DO) concentration between 4 and 5 mg/l. exhausted feed(650 ml) was pumped out under anaerobic conditions. y Constant removal of chemical oxygen demand (COD) and voltage output were considered as indicators for satisfactory formation of the biofilm and stable operating conditions. sustain microflora. y Before changing the feed. After every feeding event. the anode chamber was sparged with N2 for 2 min to maintain anaerobic microenvironment. the inoculum was allowed to settle down (30 min) and y The settled mixed inoculum (50 ml by volume) was used for subsequent experiments to y Fresh synthetic feed (650 ml) was loaded once the voltage started decreasing. . y Anolyte (synthetic feed and mixed microflora) was recirculated continuously at a rate 100 ml/min using a peristaltic pump in order to provide effective contact between the substrate and mixed consortia. y The anaerobic mixed microflora was adapted to aseptic anaerobic microenvironment after sampling the inoculum.

phenomenon and development of biofilm on the graphite surface of the anode. .Results: A steady increase in potential difference was observed with every additional feed and this might be attributed to adaptation.

Contd. which facilitated growth of AB and at the same time inhibit the functioning of MB.  For all methanogenic bacteria (MB) specific pH optimum for growth is near pH 7.0. facilitates the generation of molecular H2. The designed synthetic feed prior to feeding was adjusted to pH 5.  AB are notably less sensitive to pH variations.. This susceptible environment created.5. This made us choose AB as inoculum.0. VFA formation and persistent acidogenic metabolism encountered in the anode chamber presumably indicated a micro-environment congenial to the proliferation of H2producing AB rather than H2-consuming species. while acetogenic bacteria (AB) had lower pH optimum around 6. ....

indicates VFA consumption.  VFA: Volatile Fatty Acid. .OLR: Organic Loading Rate. OLR = {[COD0 X feed rate]/reactor volume}.  SDR: Substrate Degradation Rate.  + indicates VFA generation. SDR = {[(COD0 ± CODT) X feed rate]/reactor volume}  .

but also facilitated transfer of electrons to the anode without an exogenous mediator. but can donate electrons directly to the electrode surface by attachment. .Conclusion  This study documented the feasibility of bioelectricity generation showing that the method is environmentally sound and sustainable as substrate was wastewater .  The OLR (1/Voltage). However the last proportionality is valid in all 4 cycles.  The mixed consortia employed do not require soluble mediators. OLR CODR % and OLR SDR. OLR (1/Current).  They not only produced power in association with COD reduction.  COD removal efficiency observed enumerated the functioning of MFC as alternative wastewater treatment unit in addition to renewable energy generation.  Experimental data revealed the effectiveness of acidophilic conditions maintained for power generation within a short period of adaptation time.

.wherein renewable energy could be generated alongwith wastewater treatment.The Ultimate Conclusions  The dual activity could significantly reduce the cost associated with the current wastewater treatment methods.  This process could be effectively integrated to wastewater treatment plant.  http://www.pdf  http://www.pdf Personal%20Bioreactor/Readings_files/MFC%20revie .ias.html  http://pubs.pdf CC/b600876c  http://www.References:

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