Pitfalls in the Laboratory

Diagnosis of Parasitic Ìnfections
Þarasìto1ogy laboratory
ulms Lo usslsL cllnlcluns ln Lhe uccuruLe
dlugnosls of puruslLlc lnfecLlons
lf Lhls ls uchleved, cllnlcluns ure uble Lo glve
lmmedluLe und proper LreuLmenL Lo Lhe
puLlenL
Accurucv ls dependenL on severul fucLors
lnvolved from speclmen collecLlon Lo
speclmen exumlnuLlon
Þarasìto1ogy laboratory
osL humun puruslLes
hublLuLe Lhe lnLesLlnes und
ln Lerms of dlugnosls Lhe
mosL common speclmen
hundled ln u puruslLologv
luboruLorv ls Lhe S1OOL.
too1 Co11ectìon
As u luboruLorv dlugnosLlclun, whuL wlll vou
conslder ure lmporLunL ln reluLlon Lo sLool
collecLlon?
A. edlcuLlons
8. SLool conLulners
C. SLool ConLumlnuLlon
L. AmounL
edìcatìons
repuruLlons llke unLlbloLlcs, unLl-dlurrhelc
drugs und unLuclds cun musk Lhe presence of
puruslLes ln Lhe sLool.
CerLuln dlugnosLlc procedures uslng blsmuLh,
mlnerul oll, burlum enemu or gull bludder
dves cun produce Lhe sume effecL.
1herefore, lf sLools Lurn ouL Lo be neguLlve,
Lhere mlghL be u need for u repeuL
exumlnuLlon ufLer Lhe dlugnosLlc procedure
or Lhe LreuLmenL reglmen ls Lhrough.
too1 Contaìners
SLool sumples ure Lo be submlLLed ln covered
conLulners wlLh u wlde mouLh
1he cover prevenLs uLLrucLlng lnsecLs
speclflcullv flles, slnce flv eggs mlghL be
mlsLuken for puruslLes.
lL cun ulso prevenL fecul odor from fllllng up
Lhe whole ureu, whlle Lhe wlde mouLh ullows
one Lo geL reoulred umounL of sLool sumple
from Lhe conLulner wlLh euse.
Contamìnatìon
Soll
1olleL wuLer
Urlne
mount
1he umounL of Lhe sLool LhuL should be
submlLLed wlll depend on Lhe Lechnloues LhuL
wlll be performed.
lor rouLlne sLool exumlnuLlon (zŦ-iŦ grums)
of formed sLools (usuullv hulf of Lhe Lhumb) or
¸-6 Lublespoons of wuLerv sLool speclmens.
mount
1here ure slLuuLlons however when lL ls
necessurv Lo submlL Lhe whole sLool
movemenL llke ln cuses where Lhe luboruLorv
llkes Lo recover helmlnLhlc udulLs ufLer
LreuLmenL.
As u muLLer of fucL, enough sLool muLerlul wlll
ullow performunce of u repeuL exumlnuLlon or
oLher Lechnloues whlch wlll help conflrm u
dlugnosls.
l Þ8CL
CHOlCL Ol 1LCHNlOUL
Lvpe on Lhe puruslLes
R on reugenLsleoulpmenL
too1 Lxamìnatìon
1lme
Number of exumlnuLlon
ucroscoplc LxumlnuLlon
ConslsLencv
Color
ucus
Cross presence of puruslLes
ìcroscopìc Lxamìnatìon
1here ure z polnLs rulsed ln reluLlon Lo unv
mlcroscoplc exumlnuLlon, numelv:
roflclencv of Lhe mlcroscoplsL
OuullLv of Lhe mlcroscope
ÞseudoÞarasìtes
Alr 8ubbles
ÞseudoÞarasìtes
ucrophugeslNl us Cells
ÞseudoÞarasìtes
SLurch Crunules
ÞseudoÞarasìtes
lungul Spores
ÞseudoÞarasìtes
ollen Crulns
ÞseudoÞarasìtes
llbers of meuL or plunL orlgln

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