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Comparison NS1 with IgM captured ELISA in dengue Detection

Aji Patriajati 030.05.012

Dengue Virus ‡ Family ‡ Genus : Flaviviridae : Flavivirus ‡ Four Serotypes : ± Den-1 ± Den-2 ± Den-3 ± Den-4 .

a membrane-associated protein (M). encoding the nucleocapsid or core protein C. an envelope protein (E) and seven non-structural (NS) protein genes .Virus Morphology ‡ Single-stranded RNA genome surrounded by an icosahedral nucleocapsid and covered by a lipid envelope ‡ The genome is composed of three structural protein genes.

Dengue Virus Polyprotein .

‡ ‡ ‡ ‡ ‡ . IgM titer can be slower to rise in secondary infection. IgM antibodies detectable for up to 6 months. IgG appears approximately 2 days after symptoms appear. Secondary Infection Approximately 5% patients do not produce detectable levels of specific IgM. IgG titre significantly higher in secondary infection.Antibody Responce ‡ ‡ ‡ ‡ Primary Infection IgM antibodies appear approximately 5 days after onset of symptoms and rise for the next 1-3 weeks. IgG are detectable at approximately 14 days after onset of symptoms and are maintained for life.

and whose appearance can be variable it may be maculopapular. And ‡ Hemorrhagic manifestations ‡ ‡ ‡ ‡ ‡ . often described as retro-ocular. A rash that may present at different stages of the illness. often with sudden onset.‡ Dengue fever is an acute viral illness characterized by: Fever. Nausea and vomiting. orerythematous. Severe headache. Myalgias and arthralgias that can be very severe. petechial.

NS1 ‡ a glycoprotein is detected in high titers in patients with primary or secondary dengue infections but its function is unknown ‡ found associated with intracellular organelles or is transported through the cellular secretory pathway to the cell surface ‡ found circulating during the acute phase of the disease in sera from patients .

Dengue Virus Polyprotein .

IgM Spesific dengue ‡ dengue virus-speci c IgM as a antibodies response of dengue virus infection ‡ Produce after 3 days of fever onset usually appears on Days 4 5 of the illness ‡ Detect using ELISA method .

NS1 with IgM ELISA captured Comparison .

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Benefit NS1 ‡ NS1 AG test kit gives an overall higher sensitivity rate than the current three established diagnostic test methods for laboratory diagnosis of acute dengue infection ‡ NS1 antigen-capture ELISA gave a higher positive detection within the rst four days of illness ‡ NS1 antigen-capture ELISA has the added advantage of continuing to give good detection rates up to seven days of the illness .

NS1 ‡ Still limited by the need for sophisticated instrumentation ‡ higher technical skill which is normally only available in large hospital diagnostic laboratories .

IgM ELISA Captured ‡ The sensitivity rate of IgM assay for early diagnosis of dengue was poor in the rst three days of the illness ‡ speci c IgM was merely indicative of recent dengue infection. and not con rmative of acute dengue infection .