Tools of a Life Scientist Compound Light Microscope

Compound Light Microscope
• Robert Hooke – saw cork through a microscope he built. He was looking at soft plant tissue
– Tiny rooms – He called them cells which means “rooms”

• Anton Van Leeuwenhoek – saw tiny moving organisms in pond scum.

Compound Light Microscope
 Carry the microscope by grasping the microscope’s arm with one hand. Place your other hand under the base.  Place the microscope on a firm surface with the arm toward you.

Compound Light Microscope
 Turn the coarse adjustment knob to lower the lower the stage  Revolve the nosepiece until the ­­low power objective lens clicks into place.  Adjust the diaphragm.

Compound Light Microscope
 While looking through the eyepiece, adjust the mirror until you see a bright white circle of light. Caution: Never use sunlight as a light source.  Place a slide on the stage.

Compound Light Microscope
 Center the slide over the opening on the stage.  Use the stage clips to hold the slide in place. Caution: Glass slides are breakable. Do not let clips pop down.  Look at the stage from the side of the microscope.

Carefully turn the nose piece to rotate the low power objective lens into place.

Compound Light Microscope
 Carefully turn the coarse adjustment knob to raise the stage until the low power objective almost touches the slide (or the stage stops). THE LENS SHOULD NOT BE TOUCHING THE SLIDE.

Compound Light Microscope
 Look through the eyepiece.  Slowly turn the coarse adjustment knob until the specimen comes into focus. (it’s like a small town!)

Compound Light Microscope
 Switch to the high power objective lens by looking at the microscope from the side, carefully turning the nosepiece until the high power objective lens clicks into place.  MAKE SURE THE LENS DOES NOT HIT THE SLIDE AS YOU TURN THE NOSEPIECE

Compound Light Microscope
 Look through the eyepiece.  Slowly turn the fine adjustment knob until the specimen comes into focus.

Making a Wet-Mount slide:

 Obtain a clean slide and a clean cover slip.  Place the specimen in the center of the slide. The specimen must be thin enough for light to pass through it.

Making a Wet-Mount Slide
 Place a drop of water onto the specimen using a dropper.  Carefully place one edge of the coverslip slide so that it touches the edge of the water over the specimen at a 45 degree angle.

Making a Wet-Mount Slide
 SLOWLY lower the cover slip down over the water covered specimen  Remove air bubbles beneath the cover slip by gently tapping the cover slip with the eraser end of your pencil.  Remove excess water around the edges of the cover slip with a

Compound Light Microscope
Be prepared for “Do You See What I See?” Lab tomorrow.

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