Cell WaLer ÞoLenLlal

48lC6Ŵ1
WaLer ÞoLenLlal
W 1he relaLlve Lendency of waLer Lo move from
one area Lo anoLher
W 8epresenLed by Lhe Creek leLLer + (Þsl)
W uue Lo osmoslsţ gravlLyţ mechanlcal pressureţ
or maLrlx effecLs such as surface Lenslon
W + ƹ +
s
+ +
p
+ +
m
+ ƹ +
s
+ +
p
+ +
m
W + ƹ waLer poLenLlal of a cellŤ + ƹ 0 aL
equlllbrlum
W +
s
ƹ soluLe or osmoLlc poLenLlalŤ' always
negaLlve ln slgn
W +
p
ƹ pressure poLenLlalŤ Lurgor pressureŤ may
be (Ŵ) or (+) ln slgn
W +
m
ƹ maLrlc poLenLlalŤ due Lo waLerŴblndlng
collolds ln Lhe cellŤ negaLlve ln
Þressure ÞoLenLlal (+
p
)
W 1urgor pressure
W Maln pressure of Lhe cell conLenLs agalnsL
Lhe cell wall ln planL cells and bacLerla cells
W ueLermlned by Lhe waLer conLenL of
Lhe vacuole
W 8esulLlng from osmoLlc pressure
CsmoLlc Þressure (+
s
)
W LxerLed by Lhe flow of waLer
Lhrough a semlŴpermeable membrane separaLlng
Lwo soluLlons wlLh dlfferenL concenLraLlons of
soluLe
W Arlses from Lhe Lendency of a pure solvenL Lo
move Lhrough a semlŴpermeable membrane
and lnLo a soluLlon conLalnlng a soluLe Lo
whlch Lhe membrane ls lmpermeable
ueLermlnaLlon of WaLer ÞoLenLlal
1Ŧ LlghL dlfferenL sucrose concenLraLlons was prepared
and 73mL of each was dlspensed separaLely lnLo 130mL
beakersŦ
2Ŧ Cyllnders were placed ln covered beakers Lo prevenL
Lhem from drylng ouLŦ
3Ŧ Lach cyllnder was cuL ouL lnLo sllces of approxlmaLely
4cm and Lhe remalnlng poLaLoes were saved for ÞarL llŦ
4Ŧ Cyllnders were bloLLed wlLh paper Lowels and welghed
ln seLs of LwoŦ WelghLs were recordedŦ
3Ŧ Cne seL of cyllnders was puL ln each of Lhe beakers wlLh
sucrose soluLlons prepared prevlouslyŦ
6Ŧ 1he cyllnders were removed afLer an hour
and a halfţ bloLLed wlLh paper Lowels and
welghed agalnŦ 8esulLs were recordedŦ
7Ŧ 1he change ln welghL (AW) and Lhe percenL
change ln welghL (ƷAW) were compuLedŦ
8Ŧ 1he molal concenLraLlon of sucrose LhaL glves
0Ʒ AW was deLermlnedŦ
9Ŧ 1he +
s
ln bars of LhaL sucrose soluLlon was
compuLedŦ
10Ŧ1he waLer poLenLlal of Lhe poLaLo was
deLermlnedŦ
ormulae
W Change ln WelghL
AW ƹ Wf Ŵ Wl
W ÞercenL Change ln WelghL
ƷAW ƹ x 100
W CsmoLlc Þressure
+
s
ƹ Ŵml81
ueLermlnaLlon of SoluLe ÞoLenLlal +
s
1Ŧ Sap exLracLlon was done whereln s2Ŵ4 poLaLoes were peeledţ
chopped and placed ln a blenderŦ
2Ŧ 1he blended poLaLoes were fllLered wlLh cheesecloLh
3Ŧ lL was Lhen sLored ln a covered beakerŦ Cn Lhe freezlng polnL
deLermlnaLlonţ Peldenhaln LhermomeLer was lmmersed ln
crushed lceŴsalL baLh and Lhe readlngs were recordedŦ
4Ŧ 60mL sap was poured ln 230mL Lrlenmeyer flask wlLh magneLlc
sLlrrlng bar and Lhe LhermomeLer was Lhen lnserLedŦ
3Ŧ 1he flask was surrounded wlLh lceŴsalL baLh and was vlgorously
sLlrredŦ When Lhe LemperaLure reads abouL a degree Celslusţ Lhe
LemperaLure was ready every 10 secondsŦ
6Ŧ 1he LemperaLure vsŦ Llme was ploLLedŦ 1he Lrue freezlng polnL 1
f
ţ
was deLermlnedŦ
7Ŧ LasLlyţ Lhe pressure poLenLlalţ +
p
ţ of Lhe cells of poLaLo was
calculaLedŦ
ormulae
W 1rue reezlng ÞolnL
1
€
ƹ 1
€
Ŵ 0Ŧ0123
s
W SoluLe ÞoLenLlal
+
s
ƹ 1Ŧ22 1
€
8esulLs

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