Envirotech Centre for Research & Development New Delhi

Dissolve 4.0 gm Sodium hydroxide + 1.0 gm Sodium arsenite And make upto 1000ml with distilled water

Sulfanilamide Solution
Dissolve 20.0 gm Sulfanilamide + 50 ml of 85% phosphoric acid And make upto 1000ml with distilled water (This solution is stable for one month, if kept in refrigerator)

Hydrogen peroxide Solution
Dilute 0.2 ml of 30% H2O2 solution And make upto 250ml with distilled water

(This solution is stable for one month, if kept in refrigerator & protected for light.)

N-(1-Napthyl)-ethyenediamine dihydrochloride (NEDA) Solution
Dissolve 0.5 gm of NEDA And make upto 500ml with distilled water

(This solution is stable for one month, if kept in refrigerator & protected for light.)

Stock Sodium nitrite solution (1000ug NO2/ml)
Dissolve 1.5 gm of Sodium nitrite And make upto 1000ml with distilled water The amount of Sodium nitrite to be used, if the assay percent is less than 100%, is calculated by G=1.5/A

Where G= Amount of Sodium nitrite in gm 1.5= Gravimetric conversion factor A= Assay percentage (should be greater than 97%) (This solution is stable for six weeks, if kept in refrigerator.)

Working Sodium nitrite solution Solution A (10ug NO2/ml)
Dilute 5 ml of Stock Sodium nitrite solution

And make upto 500ml with distilled water

Solution B (1.0ug NO2/ml)
Dilute 25 ml of Solution A And make upto 250ml with absorbing solution (Prepare freshly)

Volumetric Flask Working NaNO2 Solution B Absorbing Media H2O2 Sol. Sulfanilami de Sol. NEDA Sol.

50ml Blank 0 ml

50ml Sample1 1ml

50ml Sample2 3ml

50ml Sample3 5ml

50ml Sample4 7ml

50ml Sample5 9ml

10ml 1ml 10ml 1.4ml

9ml 1ml 10ml 1.4ml

7ml 1ml 10ml 1.4ml

5ml 1ml 10ml 1.4ml

3ml 1ml 10ml 1.4ml

1ml 1ml 10ml 1.4ml

Make upto 50ml with distill water, Mix thoroughly , After 10 minutes measure absorbance of each flask at 540nm wavelength

Plot calibration curve with absorbance versus concentration of NO2

Volumetric Flask Exposed absorbing Media Absorbing Media H2O2 Sol.

50ml For Blank 0 ml 10ml 1ml

50ml For sample 10ml 0ml 1ml

Sulfanilamide Sol.
NEDA Sol.

10ml
1.4ml

10ml
1.4ml

Make upto 50ml with distill water, Mix thoroughly , After 10 minutes measure absorbance of each flask at 540nm wavelength

Total volume of air passed through Nox impinger (V, in liter) Avg. Flow Rate (lpm) X Time (minute)

Concentration (ug NO2/m3) = C x A x 1000 x D V x B x 0.82
Where: C= NO2 Curve value A= Absorbing reagent taken for sampling, ml V= Volume of sampled air B= Volume taken for sample analysis, ml D= Dilution factor 1000 for conversion from liter to m3 0.82= sampling efficiency

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