BIOPHOTONICS PROGRAM

Leon Esterowitz, Program Director ENG/CBET 703-292-7942 lesterow@nsf.gov

BIOPHOTONICS
• Photonics is the technology of generating and harnessing light and other forms of radiant energy whose quantum unit is the photon. • Biophotonics applies photonics to the fields of medicine, biology and biotechnology.

Examples of Biophotonic Topical Areas
• CONTRAST AGENTS- New classes of photonic probes and contrast agents to label structures and push the envelope of optical sensing to the limits of detection, resolution, and identification. • MOLECULAR IMAGING- Image and data fusion between optical imaging, spectroscopic techniques, and conventional imaging modalities for imaging diseases at the molecular and cellular level. • NEUROPHOTONICS- Development and application of photonic tools such as large scale parallel interfaces and interconnects for study and control of neural systems. • MICRO- and NANO-PHOTONICS- Development and application of nanoparticle fluorescent quantum-dots; sensitive, multiplexed, high-throughput characterization of macromolecular properties of cells; nanomaterials and nanodevices for biomedicine.

Selected Examples of Biophotonic Awards and Results

Functional Imaging with Diffuse Optical Wavefields
Diffuse optical tomography of brain function. Passive movement of the right arm of a premature baby stimulated brain activation as indicated by increased blood flow causing an increase in blood volume and hemoglobin oxygen saturation. AIMS OF PROJECT Develop reduced order non-linear inversion schemes that exploit MRIbased structural information. Develop new methods for processing DOT data over time. Develop fast forward model for DOT brain imaging. Eric L. Miller, Northeastern University

Large-Vertical-Displacement (LVD) Microactuator: MEMS-based Micromirrors and Microlenses for Biomedical Imaging
P.I.: Huikai Xie, Dept of ECE, Univ. of Florida; Email: hkxie@ece.ufl.edu; Tel: (352) 846-0441 Graduate Students: Ankur Jain, Shane Todd

1. Motivation
 High mortality of cancers is due to lack of early detection modalities.  Commonly used biopsy is risky and has low early detection rate.  Optical coherence tomography (OCT) is a non-invasive highresolution imaging technique, but conventional OCT is bulky and not suitable for in vivo internal organ imaging; and OCT has poor lateral resolution.

3. Design Concept
Anchor A Frame Bimorph actuator z+ Bimorph actuator zMirror A’

4. Fabricated Devices
micro-lens
(to be grown)

frame bimorph actuators mirror

2. Objective
 Design MEMS actuators for large-vertical displacement of micromirrors and microlenses, for phase-only scanning, and focusing, respectively  The micromirror can be used for axial scanning in interferometry, while the tunable microlens can be used in confocal microscopy  Develop MEMS-based confocal microscopes
Frame Silicon Polymer droplet Al Poly-Si Oxide

0.2mm vertical displacement at 6V DC, scan rate of ~ 2kHz (to be presented in Hilton Head Workshop in June 2004)

5. Research Plan
 Integrate microlens on a LVD device using polymer droplets  Integrate capacitive vibration sensors for position control  Develop MEMS-based confocal imaging probes for in vivo imaging of internal organs

Frame Single-crystal silicon

The basic idea is to use an oppositely tilted bimorph beams to compensate the tilted mirror, and thus the mirror surface will move vertically when a current is applied to both bimorph

Multimodal Miniature Microscope for Early Cancer Detection
M. Descour, University of Arizona

M. Descour, University of Arizona BES-0086736

Benign

Early Cancer

Ultra-compact microscope developed by M. Descour of the University of Arizona, together with the use of contrast agents, demonstrates the clear distinction between benign and early cancerous lesions. A pen-sized, battery-powered multi-modal miniature microscope, designed to specifically image microscopic and molecular features of pre-cancer, is the goal of this research.

Genetic Optimization of Ultrabright Ag Nanodot Biolabels Genetic Optimization of Ultrabright Ag Nanodot Biolabels
Robert Dickson, Georgia Tech & Yih-Ling Tzeng, Emory University

Robert Dickson & Yih-Ling Tzeng

Dendrimer encapsulated Ag nanodots – Idealized single biolabels
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N N N N N N N N N N N N N N N N N N N NN N N N NNN N N

N NN N N N N NN N N N N N N N N N N N N N N N N N N N NN N N NN N N N N NN N N N N N N N N N N N N N N N N NN N NN N

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• Emission from sub-nm, 2-8 atom Ag nanocluster • Water soluble due to protective poly(amidoamine) dendrimer encapsulation • Greatly reduced blinking on single molecule level • Individual nanodots easily observed with weak Hg lamp excitation (>20x brighter than organic dyes) • Multicolored and incredibly photostable – outstanding single molecule labeling potential • Conjugatable to proteins • Investigate dendrimer as vehicle for nanodot transfer to peptides

Photoactivated Coupling of Nano-particle Multilayers and Nerve Cells

Nicholas Kotov, Oklahoma State University & Massoud Motamedi, Univ Texas-Galveston

Artificial Retina Concept

• Electronics away from retinal neurons • Flexibility in electronics • Blood flow unimpeded • Easy to monitor
Mark Humayan, University of Southern California

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