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To: BioSphere

Eden Research Laboratory

Date: January 15, 2013

Update
2111 Menaul NE, Suite A Albuquerque, NM 87107 T 505.508.3994 M 505.550.9316 thomas.poth@edenresearchlab.com

From: Thomas Poth Number of pages including cover: 2 Regarding: ASTM D 5511-11 test update for PET Clear Food Containers - 27 days 624 - PET Food Containers 6020.1 34.0 2046.1 1.46 42.5 2558.9 5.03 20.0 17.0 2.47 12.1

Inculum Cumulative Gas Volume (mL) Percent CH4 (%) Volume CH4 (mL) Mass CH4 (g) Percent CO2 (%) Volume CO2 (mL) Mass CO2 (g) Sample Mass (g) Theoretical Sample Mass (g) Biodegraded Mass (g) Percent Biodegraded (%) * Adjusted Percent Biodegraded (%) 1167.6 11.6 135.4 0.10 52.6 613.9 1.21 1,000 0.0 0.40

Negative 669.4 18.1 121.5 0.09 45.1 301.8 0.59 10 8.6 0.23 -2.0

Positive 8771.8 37.0 3244.2 2.32 43.9 3848.8 7.56 10 4.4 3.80 76.9

-2.7

100.0

15.8

Biodegradation
80 60 Percent (%) 40 20 0 -20

0
NEG

8
POS

15 Time (days)

23

30

624A-1 - PET Food Containers

Biodegradation
Eden Research Laboratory

15 11 Percent (%) 8 4 0 -4

Update
2111 Menaul NE, Suite A Albuquerque, NM 87107 T 505.508.3994 M 505.550.9316 thomas.poth@edenresearchlab.com

0
NEG

15 Time (days)

23

30

624A-1 - PET Food Containers

* Outside of the ASTM D5511 method and on the assumption that the positive control (Cellulose) will fully biodegrade, all value have been proportionally adjusted.

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