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    The IC50 is the concentration of a substance that provides 50% inhibition of a biological process. It measures how much of a substance is needed to inhibit a process by half. Similarly, the EC50 is the concentration that produces 50% of a substance's maximum response in an agonist assay. To calculate IC50 or EC50, replicate data wells should be averaged and dose-response curves fitted. The four parameter logistic model is commonly used to calculate IC50, with the key result being the calculated IC50 value.

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    how to calculate ic50 value

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    The IC50 is the concentration of a substance that provides 50% inhibition of a biological process. It measures how much of a substance is needed to inhibit a process by half. Similarly, the EC50 is the concentration that produces 50% of a substance's maximum response in an agonist assay. To calculate IC50 or EC50, replicate data wells should be averaged and dose-response curves fitted. The four parameter logistic model is commonly used to calculate IC50, with the key result being the calculated IC50 value.

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    1K views1 page

    Selnikraj - IC50

    Uploaded by

    selnikraj
    The IC50 is the concentration of a substance that provides 50% inhibition of a biological process. It measures how much of a substance is needed to inhibit a process by half. Similarly, the EC50 is the concentration that produces 50% of a substance's maximum response in an agonist assay. To calculate IC50 or EC50, replicate data wells should be averaged and dose-response curves fitted. The four parameter logistic model is commonly used to calculate IC50, with the key result being the calculated IC50 value.

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    How to calculate Ic50


    The IC50, the concentration of substance that provides 50% inhibition to certain reaction, is most
    commonly used to represent the inhibitory effect of compounds on competition binding assays and
    functional antagonist assays. In simpler terms, it measures how much of a particular substance/molecule
    is needed to inhibit some biological process by 50%.
    In similar terms, there is the EC50, which is used to describe the effect of compounds on
    agonist/stimulator assays. The EC50 shows the concentration giving 50% of that compoundÅfs maximal
    response.

    Before fitting a dose-response curve to obtain the EC/IC50, each well should be converted to either
    percent activity or percent inhibition with respect to positive and negative controls. Then all replicate
    wells from a given run (including multiple plates per run) for a given concentration should be averaged
    either by taking the mean, or preferably, taking the median.

    To start the calculation of IC50/EC50 (on the web, it was described as IC50 for simplicity), input your
    data in 2 (x and y) columns into the box. Alternatively, you can import your data from a file. The file
    format should be tab-delimited text, which can be easily generated by using the software Microsoft
    Excel. After importing your data, just click "calculate".

    The four parameter logistic model (4PL), also called the Hill-Slope model, is used to calculate IC50 on
    this system. (For more detail about the calculation of IC50/EC50, see
    http://www.ncgc.nih.gov/guidance/section3.html. If you want to use other formula, see
    http://www.changbioscience.com/stat/ec50.html)

    In results window, you can see your input data, file name, date of calculation, semi-logarithmic plot of
    your data, and IC50 (important!). In addition, there is two positions indicated as "sequence". It is not
    directly related to your results. However it is better to check whether the values of both "sequence" are
    same, which means the calculation program had worked correctly.

    Mail me at selnikraj@yahoo.com

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