Surname Initial(s)

Centre
No.

Signature
Candidate No.

Paper Reference(s)
Examiner’s use only

4325/03
London Examinations IGCSE Team Leader’s use only

Biology
Paper 3 (Foundation and Higher tiers) Question Leave
Number Blank

1
Wednesday 9 November 2005 – Afternoon
2
Time: 1 hour 15 minutes
3
4
5
Materials required for examination Items included with question papers
Ruler, pencil and eraser Nil 6

Instructions to Candidates
In the boxes above, write your centre number and candidate number, your surname, initial(s) and
signature.
The paper reference is shown at the top of this page. Check that you have the correct question paper.
Answer ALL the questions in the spaces provided in this question paper.
Show all the steps in any calculations and state the units.
Calculators may be used.

Information for Candidates

There are 12 pages in this question paper. All blank pages are indicated.
The total mark for this paper is 50. The marks for the parts of questions are shown in round
brackets: e.g. (2).

Advice to Candidates
Write your answers neatly and in good English.

Total
This publication may be reproduced only in accordance with
Edexcel Limited copyright policy.
©2005 Edexcel Limited. Turn over
Printer’s Log. No.

N23057A
W850/4325/57570 5/6/5/5/5/6/800
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1. The diagram shows a piece of apparatus with two different readings.

100 100
90 90
80 80
70 70
60 60
50 50
40 40
30 30
20 20
10 10
0 0

A B

(a) (i) Write down the name of the piece of apparatus shown in the diagram.

................................................................................................................................
(1)

(ii) What does it measure?

................................................................................................................................
(1)

(b) (i) Write down the readings shown by A and B and give the unit.

Reading for A ........................................................................................................

Reading for B ........................................................................................................

(3)

(ii) What is the difference between the two readings?

................................................................................................................................
(1) Q1

(Total 6 marks)

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2. Asha wanted to test some foods.

Her teacher told her the following reagents were available.

Biuret Benedict’s ethanol iodine

(a) Choose the reagent Asha should use to test foods for starch.

.......................................................................................................................................
(1)

(b) Asha used the table below to show her results.

Complete the table by writing the colour she obtained for each food.

Food Colour obtained Starch present

bread yes

milk no

(2)

(c) Asha decided to test her foods for glucose.

(i) Describe the test she would do.

................................................................................................................................

................................................................................................................................

................................................................................................................................

................................................................................................................................
(2)

(ii) What result would she see if glucose was present?

................................................................................................................................
(1)

(iii) Suggest how she might use the results to say how much glucose was present.

................................................................................................................................

................................................................................................................................
(1) Q2

(Total 7 marks)

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3. Sunhil wanted to estimate the population size of pea plants in two fields, field X and
field Y.

He decided to use a quadrat that was 0.5 m by 0.5 m (shown in the diagram below).

frame of quadrat

He counted the number of pea plants in the quadrat. He did this at 10 different places in
each field and called his quadrats, A to J.

(a) He wrote down the number of pea plants in each quadrat for field X in a table. He
arranged his results in ascending order (lowest number first).

Results for field X

Quadrat letter H F C D J A G B I E

Number of pea plants 13 14 17 18 22 24 26 27 33 36

(i) Calculate the total number of pea plants in these 10 quadrats for field X.

Answer ....................................
(1)

(ii) The total area of these 10 quadrats is 2.5 m2 and field X has an area of 5000 m2.

Using the results for (i), calculate the estimated total population size of pea plants
for field X. Show your working.

Answer ....................................
(2)

*N23057A0412*
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(b) Sunhil then wrote down the results of the quadrat readings for field Y in a table in the
order he placed the quadrats.

Results for field Y

Quadrat letter A B C D E F G H I J

Number of pea plants 22 35 15 14 23 37 34 25 38 27

Fill in the table below for field Y. Rearrange the results in ascending order of the
number of pea plants, in the same way that Sunhil did for field X.

Quadrat letter

Number of pea plants

(1)

(c) What conclusion may be drawn about the number of pea plants in field Y compared
to field X?

.......................................................................................................................................

.......................................................................................................................................
(1) Q3

(Total 5 marks)

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4. Lee wanted to investigate the effect of different light intensities on photosynthesis.

He set up the apparatus shown in the diagram.

lamp

pond water

pondweed

(a) Name the gas that the pondweed gives off during photosynthesis.

.......................................................................................................................................
(1)

(b) Using this apparatus, suggest how Lee could change the light intensity.

.......................................................................................................................................

.......................................................................................................................................
(1)

(c) Describe how the apparatus could be used to measure how fast photosynthesis was
occurring.

.......................................................................................................................................

.......................................................................................................................................

.......................................................................................................................................
(2)

*N23057A0612*
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(d) Lee carried out the experiment at a range of light intensities, from low to very high
light intensities.

Predict the results you would expect. Sketch a graph using the axes below to show
your prediction.

Rate of
photosynthesis

low very high

Light intensity
(2)

(e) Name two factors other than light intensity that can affect the rate of photosynthesis.

1 ....................................................................................................................................

2 ....................................................................................................................................
(2) Q4

(Total 8 marks)

*N23057A0712*
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5. The picture shows a student using an exercise machine.

Seven female eighteen-year-old students took part in

an investigation using this exercise machine. They
investigated how breathing rate is affected by the
length of time they exercised.

The breathing rate of each student was measured at

rest. The students then exercised for 2 minutes and
their breathing rate was measured immediately after
this. The students then had 10 minutes to recover from
the exercise.

The students then exercised for 4 minutes and their

breathing rate was measured immediately after this.
The students had a further 10 minutes to recover.

This sequence was repeated for 6 minutes of

exercise, then 8 minutes of exercise and finally for
10 minutes of exercise.

(a) (i) Give two ways in which the students made this investigation a fair test.

1 .............................................................................................................................

................................................................................................................................

2 .............................................................................................................................

................................................................................................................................
(2)

(ii) Why is it important to make the investigation a fair test?

................................................................................................................................

................................................................................................................................
(1)

*N23057A0812*
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(b) The table shows the results of the investigation for the seven students. The mean
(average) result is also shown in the table.

Breathing rate in breaths per minute

Length of
time of
exercise Student Student Student Student Student Student Student Mean
in minutes A B C D E F G (average)

0 15 13 14 14 17 16 16 15

2 17 16 17 16 18 18 17 17

4 19 25 19 18 20 20 19 20

6 21 20 20 20 22 23 21 21

8 23 22 23 22 23 24 24 23

10 24 25 25 24 25 ? 26 25

(i) Calculate the missing value for student F after 10 minutes of exercise.

................................................................................................................................
(1)

(ii) Look at the pattern of all the results in the table. Draw a circle round one result
in the table that is anomalous (unexpected).
(1)

QUESTION 5 CONTINUES ON THE NEXT PAGE

*N23057A0912*
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(c) On the grid below, plot a line graph to show how the mean breathing rate in breaths
per minute varies with the length of time of exercise in minutes.

(5)

(d) (i) Describe the relationship between the breathing rate and the length of time of
exercise.

................................................................................................................................

................................................................................................................................

................................................................................................................................
(2)

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(ii) Using your biological knowledge, explain the relationship between breathing
rate and length of time of exercise.

................................................................................................................................

................................................................................................................................

................................................................................................................................

................................................................................................................................

................................................................................................................................

................................................................................................................................
(3)

(e) (i) Suggest one way in which the students could modify this investigation to
improve the reliability of the results.

................................................................................................................................

................................................................................................................................
(1)

(ii) Suggest one way in which the students could modify the method to improve the
accuracy of the results. Explain how this modification could improve the results.

Modification ..........................................................................................................

................................................................................................................................

Explanation ............................................................................................................

................................................................................................................................
(2) Q5

(Total 18 marks)

TURN OVER FOR QUESTION 6

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6. Water can move into or out of potato tissue by osmosis.

The photograph shows some apparatus that could be used to investigate how the
concentration of glucose solution affects this movement of water.

Describe how the apparatus in the photograph could be used to investigate movement of
water into and out of potato tissue. You may use other pieces of apparatus if you wish.

..............................................................................................................................................

..............................................................................................................................................

..............................................................................................................................................

..............................................................................................................................................

..............................................................................................................................................

..............................................................................................................................................

..............................................................................................................................................

..............................................................................................................................................

..............................................................................................................................................

.............................................................................................................................................. Q6

(Total 6 marks)
TOTAL FOR PAPER: 50 MARKS

END

*N23057A01212*
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