Kubala L, Ruzickova J, Nickova K, Sandula J, Ciz M, Lojek A Institute of Biophysics Academy of Sciences of the Czech Republic, Krlovopolsk 135,

612 65 Brno, Czech Republic. Carbohydrate Research [2003, 338(24):2835-2840] Type: Journal Article, Research Support, Non-U.S. Gov't DOI: 10.1016/j.carres.2003.09.007 Abstract (1-->3)-beta-D-glucans are known as potent inductors of humoral and cell-mediated immunity inhumans and animals. (1-->3)-beta-D-glucans isolated from various sources differ in their chemical structure and physical parameters and consequently in their immunomodulatory potential. In this study the immunomodulatory activity of two (1-->3)-beta-Dglucans schizophyllan (SPG) and carboxymethylglucan (CMG) was determined and compared on human blood leukocytes in vitro. Both SPG and CMG activated blood phagocytes and lymphocytes as demonstrated by increased whole blood production of reactive oxygen species, by increased production of pro-inflammatory cytokines IL-6, IL-8, and TNF-alpha, by increased surface expression of CD69 on lymphocytes, and by altered expression of CD11b and CD62L on polymorphonuclear leukocytes and monocytes. SPG demonstrated a significantly higher potential to stimulate blood phagocytes and production of selected pro-inflammatory cytokines than CMG. The higher potency of SPG to stimulate human blood phagocytes in vitro could be caused by factors such as higher branching frequencies or neutral polymer charge of SPG or different conformation in solution if compared with CMG.

Immunopotentiation of intraepithelial lymphocytes in the intestine by oral administrations of beta-glucan. Tsukada C, Yokoyama H, Miyaji C, Ishimoto Y, Kawamura H, Abo T. Source Department of Immunology, Niigata University School of Medicine, Japan. Abstract Mice were orally administered with beta-glucan, isolated from baker's yeast, daily for one week (25mg/day/mouse) and several immunoparameters in the digestive tract were examined. The most prominent change was an increase in the number of intraepithelial lymphocytes (IEL) in the intestine, although the number of lymphocytes in the liver remained unchanged. The absolute number of both alphabetaT cells and gammadeltaT cells expressing CD8 antigens increased among IEL in the intestine. Primarily, liver lymphocytes showed a spontaneous production of Type 0 cytokine (simultaneous production of IFNgamma and IL-4) while IEL did not produce any cytokines without stimulation. However, mice administered with beta-glucan produced Type 1 cytokine, namely, production of IFNgamma alone. These results suggest that beta-glucan may be an important potentiator for mucosal immunity in the digestive tract.

Kubala L, Ruzickova J, K Nickova, Sandula J, CIZ M, A Lojek Institut Biofisika Akademi Ilmu Pengetahuan Republik Ceko, Krlovopolsk 135, 612 65 Brno, Republik Ceko. Karbohidrat Penelitian [2003, 338 (24) :2835-2840] Tipe: Jurnal Pasal, Dukungan Penelitian, Non-AS. Gov't DOI: 10.1016/j.carres.2003.09.007 abstrak (1 -> 3)-beta-D-glukan yang dikenal sebagai induktor ampuh inhumans imunitas humoral dan sel-dimediasi dan hewan. (1 -> 3)-beta-D-glukan diisolasi dari berbagai sumber berbeda dalam struktur kimianya dan parameter fisik dan akibatnya dalam potensi imunomodulator mereka. Dalam penelitian ini aktivitas imunomodulator dari dua (1 -> 3)-beta-D-glukan schizophyllan (SPG) dan carboxymethylglucan (CMG) ditentukan dan dibandingkan pada leukosit darah manusia in vitro. Kedua SPG dan CMG fagosit darah aktif dan limfosit seperti yang ditunjukkan oleh produksi darah meningkat seluruh spesies oksigen reaktif, dengan peningkatan produksi sitokin pro-inflamasi IL-6, IL-8, dan TNF-alpha, dengan ekspresi permukaan peningkatan CD69 pada limfosit , dan dengan ekspresi yang berubah CD11b dan CD62L pada leukosit PMN dan monosit. SPG menunjukkan secara signifikan lebih tinggi potensial untuk merangsang fagosit darah dan produksi terpilih pro-inflamasi sitokin dibandingkan CMG. Potensi tinggi dari SPG untuk merangsang fagosit darah manusia in vitro dapat disebabkan oleh faktor-faktor seperti frekuensi bercabang yang lebih tinggi atau biaya polimer netral konformasi SPG atau berbeda dalam larutan jika dibandingkan dengan CMG. Immunopotentiation limfosit intraepithelial dalam usus oleh administrasi oral beta-glukan. Tsukada C, H Yokoyama, Miyaji C, Ishimoto Y, H Kawamura, Abo T. sumber Departemen Imunologi, Niigata University School of Medicine, Jepang. abstrak Tikus oral dengan beta-glucan, terisolasi dari ragi roti, setiap hari selama satu minggu (25mg/day/mouse) dan immunoparameters beberapa saluran pencernaan diperiksa. Perubahan yang paling menonjol adalah peningkatan jumlah limfosit intraepithelial (EI) dalam usus, meskipun jumlah limfosit dalam hati tetap tidak berubah. Jumlah absolut dari kedua sel alphabetaT dan sel gammadeltaT mengekspresikan CD8 antigen meningkat di antara EI dalam usus. Terutama, limfosit hati menunjukkan produksi spontan Tipe 0 sitokin (produksi simultan IFNgamma dan IL-4), sementara EI tidak menghasilkan sitokin tanpa stimulasi. Namun, tikus diberikan dengan beta-glukan yang dihasilkan Type 1 sitokin, yaitu, produksi IFNgamma saja. Hasil ini menunjukkan bahwa beta-glucan mungkin merupakan potentiator penting untuk kekebalan mukosa di saluran pencernaan. PMID: 12742376 [PubMed - diindeks untuk MEDLINE] Baru! Klik kata di atas untuk mengedit dan melihat terjemahan alternatif. Tutup