Bioreactor Design Fundamentals

The most widely used Industrial Bioreactors are: Batch Reactors Continuous Reactors Continuous Reactors With Recycle Semi Continuous Reactors (Fill/Draw Reactors including Sequencing Batch Reactors)

Bioreactor Design Fundamentals (Contd)

DEFINITIONS Limiting Substrate The substrate or substrate(s) (energy source, carbon source and/or nutrient source) which is/are first exhausted in batch growth. This substrate(s) has a direct influence on the kinetics of cell growth.

Bioreactor Design Fundamentals (Contd)

DEFINITIONS In Excess The substrate or substrate(s) (energy source, carbon source and/or nutrient source) which is/are NOT exhausted at the end of batch growth. This substrate(s) has NO EFFECT on the kinetics of cell growth.

Bioreactor Design Fundamentals (Contd)

Bioeactor Design Fundamentals (Contd) k1* and A are combined to form another constant k and the rate expression becomes: RATE = k (S / KS + S) Where k = maximum rate of transport of S ( mol / h) When S is much larger than KS : RATE k f (S) When S is similar to KS : RATE = f (S)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

THE LAG PHASE

Bioreactor Design Fundamentals (Contd)

THE LAG PHASE

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)

Bioreactor Design Fundamentals (Contd)