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Blood samples are centrifuged at 3000 rpm for 10 min to generate packed
RBCs
RBCs were washed thrice with 0.9% saline & lysed by addition of 15 ml ice
cold deionized water with vigorous shaking
Sample preparation for HPB-HB adduct
assay by GC/MS
100l lysed RBC was placed in 7 ml borosilicate glass tube
Analysis of samples
Helium carrier gas was used with flow rate 1.3 ml/min
Injection of sample with split less injection mode with injection port
temperature at 280 C
• Ogawa,M., Oyama, T., Isse, T., Yamaguchi, T., Murakami, T., Endo, Y. and
Kayamoto, T. (2006) Hemoglobin adducts as a marker of exposure to chemical
substances, especially PRTR class I designated chemical substances. J. Occup.
Health., 48(5):314-328
• Schaffler G., Betz C., and Richter E.(1993) Mass spectrometric analysis of tobacco-
specific haemoglobin adducts. Environmental Health Perspectives, 99:187-189
• Shah, T., Sullivan, K. And Carter, J.(2006) Sudden infant death syndrome and
reported maternal smoking during pregnancy. Am. J. Public. Health., 96(10):1757-
1759
• Wagenknecht, L.E., Burke, G.L., Perkins, L.L., Haley, N.J. and Friedman G.D.
(1992) Misclassification of smoking status in the CARDIA study: a comparison of
self-report with serum cotinine levels. Am. J. Public. Health, 82(1):33-36
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