Introduction

Adenosine administration improves high

blood pressure, plasma lipid, and glucose Driselase-treated fraction of rice bran—the filtrate of rice bran treated with plant cell wall-
degrading enzyme—has a beneficial effect on lowering hypertension and improving lipid
levels in stroke-prone spontaneously profiles. (Ardiansyah et al., 2006. J. Agric. Food Chem. 54, pp. 1914-1920).

hypertensive rat
Adenosine has physiological functions;
1. Therapy neonatal refractory pulmonary hypertension.
Ardiansyah, Hitoshi Shirakawa, Takuya Koseki1, Michio Komai
2. Attenuates proliferation in both human and rat glomerular
mesangial cell.
3. Decrease in blood glucose and insulin level after oral
Adenosine administration.

Laboratory of Nutrition, Graduate School of Agricultural Science Objectives

Tohoku University To establish whether oral chronic and single administration of adenosine have
1 Faculty of Agriculture, Yamagata University
physiologically affected on improvement hypertension, hyperlipidemia and hyperglycemia
in SHRSP.

Oral glucose tolerance test

Methods
200 Plasma glucose 1.6 Plasma insulin
180
Glucose (mg/dL)

9 10 11 12 13 （Week） 1.2

Insulin (ng/mL)
160 *
* *
one week 140
0.8
acclimation 1.SHRSP administrated by 120
distilled water as control 100
group and adenosine (10 1.Sacrificed 0.4
80 Control Adenosine
mg/L) as adenosine 2.Blood and organ
OGTT after 16 h fasting; group. collecting for further 60 0.0
analyses 0 30 60 120 0 30 60 120
Control (1 mL 20% glucose 2.Water intake/food intake
solution) Time after administration (minutes) Time after administration (minutes)
(F2 diet; Funabashi Co.)
Adenosine (1 mL 20% measured every day. iAUC glucose iAUC insulin
glucose solution containing †
3.Systolic blood pressure 6000
40 mg/mL adenosine) * Control
and body weight 5000 50
iAUC glucose

measured every week. 40 Adenosine

iAUC insulin

4000
30
3000
20 means + SE, n = 4
2000
The rats were housed individually in stainless steel cages under a controlled atmosphere 10
1000 * (P<0.05)
(temperature, 23 + 2 oC; humidity 50 + 10 %; 12 h light-dark cycle, 08:00 am-08:00 pm) 0 †
0 (P<0.01)

Growth parameter
Systolic blood pressure
Water intake
water intake (mL/day)

There were similar of final 40 Plasma nitric oxide

body weight, body weight 260
gain and food intake for two 30
Adenosine intake;
Blood pressure (mmHg)

groups during experimental

20 0.31 + 0.02 mg/day 6 *
period. 220
5
NO (μmol/L)

10 † 4
180 † 3
0
Control Adenosine
2
3 140 1
Relative organs weight Control Adenosine 0
relative weight (%)

Control Adenosine
2 * 100
10 11 12 13
means + SE, n = 4
age (weeks)
1 * (P<0.05) †
* (P<0.05) (P<0.01); means + SE, n = 4

0
Liver Kidney Heart Epididymal fat

1
 80 Plasma lipid levels Plasma glucose
* Plasma FFA Plasma insulin
* † 200 †
0.4
Plasma lipid (mg/dL)

60 4 *

Glucose (mg/dL)
160

Insulin (ng/mL)
FFA (mEq/L)
0.3
* 120 3
40
0.2 2
80
* 0.1
20 40 1
0.0 0 0
0
TG TC HDL-Cholesterol LDL-Cholesterol Plasma leptin Plasma adiponectin
70 *
* 12

Adiponectin (μg/mL)
3
Liver lipid (mg/g tissue)

60 Liver lipid levels

Leptin (ng/mL)
9
50 Control Adenosine 2
40 6
* †
30 means + SE, n = 4 1 3
†
20 * (P<0.05) (P<0.01)
0 0
10
0 * (P<0.05) † (P<0.01); means + SE, n = 4
Control Adenosine
Total lipid TG TC

One week after

12 weeks of SHRSP/Izm
Single oral
one week Oral administration of distilled water as
administration of control group and adenosine* group after
acclimation
adenosine 16 h of fasting
Oral administration by distilled water as control
group and adenosine* group after 16 h of fasting * 10.0 mg/kg body weight
After 2 h
* 10.0 mg/kg body weight

Measurement of BP (0, 1, 2, 4, and 6 h) Sacrificed

240 Plasma nitric oxide Blood plasma level

Systolic blood pressure 200 *
Blood pressure (mmHg)

4
Blood plasma (mg/dL)

220 *
160
NO (μmol/L)

3 †
200 120
2 80
180
† * † means + SE, n = 4
1 40
160 Control Adenosine †
* (P<0.05) (P<0.01)
0 0
140 TG TC Glucose
0 1 2 4 6
†
Time after administration (hour) Control Adenosine * (P<0.05) (P<0.01); means + SE, n = 4

Conclusion
Adenosine

Insulin sensitivity
&
Adiponectin level

Improvement Improvement
Blood pressure
glucose metabolism lipid metabolism

Prevention of metabolic syndrome related diseases