Welcome to Scribd, the world's digital library. Read, publish, and share books and documents. See more
Download
Standard view
Full view
of .
Look up keyword
Like this
1Activity
0 of .
Results for:
No results containing your search query
P. 1
Lecture 2[1]

Lecture 2[1]

Ratings: (0)|Views: 11|Likes:
Published by MuhammadFakhriAimi

More info:

Published by: MuhammadFakhriAimi on Nov 10, 2011
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as PPT, PDF, TXT or read online from Scribd
See more
See less

01/26/2015

pdf

text

original

 
At the end of this lecture, students will be ableto:
define sequencing
Compare between the enzymatic and chemicalsequencing method
Explain how chemical sequencing method iscarried out
Describe the differences of automatedsequencing method compared to the
conventional sanger’s dideoxy method
 
Lists applications of sequencing
Learning Outcomes:
 
Sequencing of DNA
DNA sequencing is the determination of the precisesequence of nucleotides in a sample of DNA
Sequencing is principally classified by two methods:
Enzymatic sequencing
 
(or named after its developers 'Sanger-Coulson- Sequencing')
 
Chemical sequencing method
 
(or named 'Maxam-Gilbert-Sequencing' after its developers)
 
Maxam & Gilbert's method (1976)
-chemical cleavage method using doublestranded DNA-involves 2 steps catalytic process
2 chemicals that selectively attackspurines or pirimidines (step 1)
Piperidine will catalyze phosphodiesterbond cleavage where the base has beendisplaced (step 2)

You're Reading a Free Preview

Download
scribd
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->