SELECTION GUIDE – Autoradiography Methods and Materials for Life Science Applications

Application Result Required Label Method Film Support products

Southern/Northern blots; High speed and sensitivity; Accurate quantification 32P Pre-flash with screens at -70°C Hyperfilm MP Sensitize, Hyperscreen
Colony/plaque blots
Maximum speed and sensitivity 32P Screens at -70°C BioMax MS BioMax TranScreen HE
Optimum resolution 32P/35S Direct autoradiography Hyperfilm bmax/BioMax MR
Non-radioactive; quantification ECL Pre-flash and direct detection Hyperfilm ECL Sensitize
Slot blots High speed and sensitivity; accurate quantification 32P Pre-flash with screens at -70°C Hyperfilm MP Sensitize, Hyperscreen
Maximum speed and sensitivity 32P Screens at -70°C BioMax MS BioMax TranScreen HE
Non-radioactive, quantification ECL Pre-flash and direct detection Hyperfilm ECL Sensitize
Dideoxy sequencing High speed 32P Direct autoradiography Hyperfilm MP/BioMax MR
Optimum resolution and maximum speed for difficult 32S Direct autoradiography Biomax MR
sequences
Good resolution and speed for routine work 32S Direct autoradiography Hyperfilm MP
Cycle sequencing Optimum resolution and maximum speed for difficult 32P Direct autoradiography BioMax MR
sequences
Good resolution and speed for routine work 33P Direct autoradiography Hyperfilm MP
Protein synthesis, lysates Maximum speed 35S/14C/3H Pre-flash and fluorography at -70°C Hyperfilm MP Amplify, Sensitize
Optimum resolution 125I Indirect autoradiography BioMax MS BioMax TranScreen HE
Direct autoradiography Hyperfilm 3H
Western blots Maximum speed and sensitivity 125I Pre-flash and screens at -70°C Hyperfilm MP/BioMax MS Sensitize, Hyperscreen, BioMax TranScreen HE
Optimum resolution 35S/14C Direct autoradiography Hyperfilm bmax/BioMax MR
Non-radioactive/maximum sensitivity ECL Pre-flash and direct detection Hyperfilm ECL Sensitize
In situ hybridization Macroscale in situ optimization of parameters 32P Direct autoradiography Hyperfilm MP
Receptor studies Localization at cellular level 33P/35S Direct autoradiography Hyperfilm bmax/BioMax MR
– intermediate resolution
– high resolution 33P/35S Micro autoradiography Hypercoat emulsion LM-1
Localization at sub-cellular level 125I/3H Direct autoradiography Hyperfilm 3H
– intermediate resolution
– high resolution 125I/3H Micro autoradiography Hypercoat emulsion EM-1
Whole body autoradiography Optimum resolution 125I/3H Direct autoradiography Hyperfilm 3H
High resolution 35S/14C Direct autoradiography Hyperfilm bmax/BioMax MR