Ordering Information

For more information about our Ion exchange

Columns Code No. Media Pack size Code No.

Mini Q PC 3.2/3* 17-0686-01 SOURCE 15Q 10 ml 17-0947-20

Mini S PC 3.2/3* 17-0687-01 50 ml 17-0947-01

chromatography columns and

Mini Q PE 4.6/50 17-5004-01 SOURCE 15S 10 ml 17-0944-10
Mini S PE 4.6/50 17-5005-01 50 ml 17-0944-01

columns and media

Mono Q PC 1.6/5* 17-0671-01 SOURCE 30Q 10 ml 17-1275-10
Mono Q HR 5/5 17-0546-01 50 ml 17-1275-01
Mono Q HR 10/10 17-0556-01 SOURCE 30S 10 ml 17-1273-20

media range, see our homepage:

Mono Q HR 16/10 17-0506-01 50 ml 17-1273-01
Mono S PC 1.6/5* 17-0672-01 Q Sepharose High Performance 75 ml 17-1014-01
Mono S HR 5/5 17-0547-01 SP Sepharose High Performance 75 ml 17-1087-01
Mono S HR 10/10 17-0557-01 Q Sepharose Fast Flow 25 ml 17-0510-10
Mono S HR 16/10 17-0507-01 300 ml 17-0510-01
RESOURCE Q 1 ml 17-1177-01 SP Sepharose Fast Flow 25 ml 17-0729-10
RESOURCE Q 6 ml 17-1179-01 300 ml 17-0729-01
SOURCE 15Q PE 4.6/100 17-5065-01 DEAE Sepharose Fast Flow 25 ml 17-0709-10
RESOURCE S 1 ml 17-1178-01 500 ml 17-0709-01
RESOURCE S 6 ml 17-1180-01 CM Sepharose Fast Flow 25 ml 17-0719-10
SOURCE 15S PE 4.6/100 17-5067-01 500 ml 17-0719-01

WWW.apbiotech.com
HiTrap Q Sepharose High Performance 5 x 1 ml 17-1153-01 ANX Sepharose 4 Fast Flow
HiTrap Q Sepharose High Performance 5 x 5 ml 17-1154-01 (high sub) 25 ml 17-1287-10
HiTrap SP Sepharose High Performance 5 x 1 ml 17-1151-01 500 ml 17-1287-01
HiTrap SP Sepharose High Performance 5 x 5 ml 17-1152-01 Q & SP Sepharose XL on request
HiLoad 16/10 Q Sepharose High Performance 17-1064-01 Q & SP Sepharose Big Beads on request
HiLoad 26/10 Q Sepharose High Performance 17-1066-01 STREAMLINE DEAE & SP on request
HiLoad 16/10 SP Sepharose High Performance 17-1065-01 STREAMLINE Q XL & SP XL on request
HiLoad 26/10 SP Sepharose High Performance 17-1137-01
Handbook:
HiLoad 26/10 SP Sepharose High Performance 17-1138-01
Ion Exchange Principles and Methods 18-1114-21
HiTrap IEX Selection Kit** 7 x 1 ml 17-6002-33
HiTrap Q Sepharose Fast Flow 5 x 1 ml 17-5053-01
HiTrap Q Sepharose Fast Flow 5 x 5 ml 17-5156-01 Larger pack sizes for media are available on request.
HiTrap SP Sepharose Fast Flow 5 x 1 ml 17-5054-01
HiTrap SP Sepharose Fast Flow 5 x 5 ml 17-5157-01 *PC colums are designed for SMART System. Precision Column
HiTrap DEAE Sepharose Fast Flow 5 x 1 ml 17-5055-01 Holder (17-1455-01) is required for attachment to ÄKTApurifier
HiTrap DEAE Sepharose Fast Flow 5 x 5 ml 17-5154-01 and other HPLC systems.
HiTrap CM Sepharose Fast Flow 5 x 1 ml 17-5056-01 **HiTrap IEX Selection Kit: Q Sepharose Fast Flow, DEAE Sepharose
HiTrap CM Sepharose Fast Flow 5 x 5 ml 17-5155-01 Fast Flow, SP Sepharose Fast Flow, CM Sepharose Fast Flow,
HiTrap ANX Sepharose 4 Fast Flow (high sub) 5 x 1 ml 17-5162-01 ANX Sepharose 4 Fast Flow (high sub), Q Sepharose XL and
HiTrap ANX Sepharose 4 Fast Flow (high sub) 5 x 5 ml 17-5163-01 SP Sepharose XL pre-packed in 1 ml HiTrap columns.
HiTrap Q XL 5 x 1 ml 17-5158-01
HiTrap Q XL 5 x 5 ml 17-5159-01
HiTrap SP XL 5 x 1 ml 17-5160-01
HiTrap SP XL 5 x 5 ml 17-5161-01
HiPrep 16/10 Q XL 17-5092-01
HiPrep 16/10 SP XL 17-5093-01 ÄKTA, SMART, HiTrap, HiLoad, Sepharose, Sephadex, Mono S, Mono Q,
HiPrep 16/10 CM 17-5091-01 Mono P, MiniBeads, MonoBeads, Mini S, Mini Q, RESOURCE, BioProcess,
HiPrep 16/10 DEAE 17-5090-01 STREAMLINE, SOURCE, BioPilot, FineLINE, Polybuffer and HiPrep are
HiLoad 16/10 Q Sepharose Fast Flow 17-1060-01 trademarks of Amersham Pharmacia Biotech Limited or its subsidiaries
HiLoad 26/10 Q Sepharose Fast Flow 17-1062-01
Amersham is a trademark of Nycomed Amersham plc
HiLoad 16/10 SP Sepharose Fast Flow 17-1135-01
Pharmacia and Drop Design are trademarks of Pharmacia & Upjohn Inc
HiLoad 26/10 SP Sepharose Fast Flow 17-1136-01
Tris is a trademark of Union Carbide Chemical and Plastics Co. Selection guide and product profile
All goods and services are sold subject to the terms and conditions of sale of the
BioProcess company within the Amersham Pharmacia Biotech group which supplies them.
A copy of these terms and conditions is available on request.
Media

BioProcess Media - Media for bioprocessing

Secure Supply
Validated Manufacture
Regulatory Support
Large capacity production integrated with clear ordering and delivery routines means © Amersham Pharmacia Biotech UK Limited 2000 - All rights reserved
BioProcess™ media are available in the right quantity, at the right place, at the right time.
We can assure future supplies of BioProcess Media, making them a safe investment for Amersham Pharmacia Biotech UK Limited Amersham Place Little Chalfont
your long term production. Buckinghamshire England HP7 9NA
Amersham Pharmacia Biotech AB SE-751 84 Uppsala Sweden
Produced following validated methods and tested under strict control, BioProcess Media fulfill high performance specifications.
A certificate of analysis is available with each order. Amersham Pharmacia Biotech Inc 800 Centennial Avenue PO Box 1327
Piscataway NJ 08855 USA
Regulatory Support Files contain details of performance, stability, extractable compounds and analytical methods available.
Amersham Pharmacia Biotech Europe GmbH Munzinger Strasse 9,
The essential information in these files is an invaluable starting point for process validation, as well as support for clinical and
marketing applications submitted to regulatory authorities. D-79111 Freiburg, Germany

From Capture to Polishing
High Productivity
Sanitization/CIP
Scalability
Specific BioProcess Media have been designed for each chromatographic stage in a process from Capture to Polishing.
Using BioProcess Media for every stage results in an easily validated economic process.
High flow rates, high capacity and high recovery contribute to the overall economy of an industrial process.
All BioProcess Media have high chemical stability to allow efficient cleaning and sanitization procedures.
Packing methods are established for a wide range of scales and compatible large scale columns and equipment are available. 18-1127-31
Edition AC
 Technical information
Table 1. Buffer tables.
Principle of Ion Exchange Chromatography Sample preparation Buffer substances for cation exchange chromatography Optimization parameters (in priority order) Storage of gels and columns
Ion exchange chromatography is capable of separating molecules that have only Correct sample preparation extends the life of the column and ensures good resolution. pKa pH Substance Conc. dpKa/ Counter-ion Comments 1. Scout for optimum pH: use automatic pH scouting, if available. Begin 0.5 - 1 pH Never freeze ion exchange media. Freezing can disrupt the bead structure.
(25°C) interval (mM) dT (°C)
slight differences in charge to give a very high resolution separation. The technique is To ensure efficient binding during sample application, samples should be at the same unit away from the isoelectric point of the target molecule if known. Recommended antimicrobial agents:
2.00 1.5-2.5 Maleic acid 20 Na+ Dicarboxylic acid
most suited for the capture or intermediate steps in a purification. Fractions are pH and ionic strength as the starting buffer. Samples must be free from particulate 2. Scout for optimum ion exchanger: use automatic column scouting, if available,
2.88 2.38-3.38 Malonic acid 20 Na+/Li+ Dicarboxylic acid 20% ethanol or 0.01 M NaOH
collected in purified, concentrated form. matter, particularly when working with bead sizes of 34 µm or less, and free from 3.13 2.63-3.63 Citric acid 20 -0.0024 Na+ Dicarboxylic acid on small columns e.g. HiTrap IEX Selection Kit.
visible contamination by lipids. 3.81 3.6-4.3 Lactic acid 50 Na+ N.B. media containing SP ligands should be buffered with 0.2 M sodium acetate
The separation is based on the reversible interaction between a charged molecule and 3. Scout for steepest gradient which gives acceptable resolution at the selected pH.
*3.75 3.8-4.3 Formic acid 50 +0.0002 Na+/Li+ when stored in 20% ethanol.
an oppositely charged chromatographic medium. Molecules bind as they are loaded Suggestions for clean up procedures: If automatic scouting is not available, begin with 0 - 50% elution buffer including
*4.21 4.3-4.8 Butanedioic acid 50 -0.0018 Na+
onto the column. Conditions are then altered so that the bound substances are eluted Gel filtration *4.76 4.8-5.2 Acetic acid 50 +0.0002 Na+/Li+ 1 M NaCl and a gradient volume of 10 - 20 column volumes. Storage of unused media
differentially. Elution is usually performed by changes in salt concentration or pH. Sephadex™ G-25, e.g. packed in a HiTrap™ Desalting column for sample volumes up *5.68 5.0-6.0 Malonic acid 50 Na+/Li+ Dicarboxylic acid
4. Scout for highest flow rate which maintains resolution and minimizes separation • closed containers at +4°C to +25°C
Changes are made stepwise or with a continuous gradient. Most commonly, *7.20 6.7-7.6 Phosphate 50 -0.0028 Na+ Often needs
to 1.5 ml or a HiPrep™ 26/10 Desalting column for sample volumes up to 15 ml, time. Check recommended flow rates for the specific medium.
samples are eluted with salt (NaCl), using the gradient outlined in Fig. 1. purification • ensure unused gel is protected against bacterial growth e.g. in 20% ethanol or
removes lipids, salts and others small molecules. Sample is transferred into the correct before use 5. For larger scale purifications separation times and buffer consumption are other antimicrobial agent
starting buffer for binding to the ion exchanger. Ideal at all scales of work, sample *7.55 7.6-8.2 HEPES 50 -0.0140 Na+/Li+ Zwitterionic
sample gradient further reduced by transfer to a stepwise elution as shown in Fig. 3 (after
equilibration wash re-equilibration loading can be 20 - 30% of total column volume. *8.35 8.2-8.7 BICINE 50 -0.0180 Na+ Zwitterionic
application elution optimization of parameters). Storage of used media
high salt wash Filtration • store between +4°C to +8°C
high salt wash
1M
2-4 cv
1 µm filters for 90 µm media Buffer substances for anion exchange chromatography
2 cv • add antimicrobial agent to prevent bacterial growth
0.45 µm filters for 3, 10, 15, 30 and 34 µm media
pKa pH Substance Conc. dpKa/ Counter-ion Comments
unbound molecules elute, 0.22 µm filters for sterile filtration or extra clean samples elution
Storage of packed columns
(25°C) interval (mM) dT (°C) of target
UV signal returns to baseline
Centrifugation *4.75 4.5-5.0 N-methyl 20 -0.015 Cl-
molecule
• store between +4°C to +8°C (used column) or +4°C to +25°C (unused column).
1-2 cv
[NaCl]

10 000 g for 15 minutes piperazine elution of

• add antimicrobial agent

[NaCl]
Appropriate for small samples or those which adsorb to filters *5.68 5.0-6.0 Piperazine 20 -0.015 Cl-/HCOO- sample unwanted
*5.96 5.5-6.0 L-histidine 20 Cl- injection material
• clean thoroughly before long term storage following instructions supplied
Expanded bed adsorption (STREAMLINE™) *6.46 5.8-6.4 bis-Tris 20 -0.017 Cl -
volume
1-2 cv
10-20 cv with column
A technique which requires no sample clean up and is used as the first clean up and *6.80 6.4-7.3 bis-Tris propane 20 Cl-
purification capture step in large scale purification. *7.76 7.3-7.7 Triethanolamine 20 -0.020 Cl-/CH3COO- equilibration re-equilibration
2 cv 2 cv
0
*8.06 7.6-8.0 Tris 20 -0.028 Cl- Often needs 2 cv 2 cv Chromatofocusing
Column volumes [cv] purification
Figure 1. Column preparation before use and Column volumes [cv]
Chromatofocusing is a technique which separates biomolecules on the basis of
Selectivity *8.52 8.0-8.5 N-methyl- 50 -0.028 SO2-/Cl-/ especially Figure 3.
pH of mobile phase
diethanolamine CH3COO- sensitive to differences in their isoelectric points. Sample is bound to a specialized chromato-
Pre-packed columns graphic medium and components are eluted using Polybuffer™ to generate a pH
Abs Abs Abs Abs temperature
To increase speed and efficiency in method development small pre-packed columns *8.88 8.4-8.8 Diethanolamine 20 at pH 8.4 -0.025 Cl- change. gradient through the column. Resolution of proteins with differences of 0.05 pH
The surface charge of proteins and peptides (e.g. HiTrap IEX Selection Kit) are used for media scouting and method optimiza- 50 at pH 8.8 Cleaning, sanitization and sterilization unit (PBE) or 0.02 pH unit (Mono P™) can be achieved. Chromatofocusing is used
V V V V
tion. Pre-packed columns ensure reproducible results and highest performance. *8.64 8.5-9.0 1,3-diamino- 20 -0.031 Cl -
depends on the amino acid content and on partially purified samples and is not suitable for samples in which components
+ propane Cleaning-in-place (CIP) is the removal of very tightly bound, precipitated or denatured
varies according to pH. IEX can be repeated Column packing *9.50 9.0-9.5 Ethanolamine 20 -0.029 Cl - precipitate at their isoelectric points since these will tend to precipitate on the
Cation substances from previous purifications. Substances, such as lipids or denatured
at different pH values to separate several *9.73 9.5-9.8 Piperazine 20 -0.026 Cl- column. For further information ask for the handbook Chromatofocusing with
Surface net charge

When packing a column the following guidelines apply at all scales of operation: proteins, may remain in the column bed instead of being eluted during the wash,
proteins which have distinctly different *10.47 9.8-10.3 1,3-diamino- 20 -0.026 Cl- Polybuffer and PBE (18-1009-07).
0 pH
- Column dimensions = typically 5 - 15 cm bed height causing high back pressures or reduced flow rates. Specific CIP should be designed
charge properties. Fig. 2. shows the effect of propane
Anion
- Quantity of gel = estimate amount of gel required to bind the sample, use five 11.12 10.6-11.6 Piperadine 20 -0.031 Cl- depending on the type of contaminants. NaOH is often the most efficient cleaning
pH on protein elution patterns. times this amount to pack a column 12.33 11.8-12.0 Phosphate 20 -0.026 Cl-
Product Ordering Information Working Maximum
agent. pH range flow rate
-
* Recommended on the basis of experiments performed in our laboratories.
See product packing instructions for detailed information on specific media. Sanitization is the inactivation of microbial populations, used to reduce the risk of Prepacked columns Code No Column dimensions
Abs Abs Abs Abs
contaminating the purified product with viable micro-organisms. NaOH is the most Bulk media diam.(mm)/h(mm) /

V V V V
Buffer preparation Table 2. Volatile buffer systems. commonly used sanitization agent. Pack size

Figure 2. Mono P HR 5/5 17-0611-01 5/50 3 - 11 0.5 - 1.5 ml/min

pH Substance Counter-ion Sterilization is the destruction of all forms of microbial life in the system.
Buffering ion Mono P HR 5/20 17-0548-01 5/200 3 - 11 0.5 - 1.5 ml/min
2.0 Formic acid H+ CIP, sanitization and sterilization protocols for SOURCETM and SepharoseTM
Polybuffer exchanger
Choice of ion exchanger It is recommended to use a buffering ion with the same charge as the gel and with a 2.3-3.5 Pyridine/formic acid HCOO- (excluding pre-packed columns, Sepharose XL and STREAMLINE) are summarized
pKa within 0.6 pH units of the working pH. 3.0-5.0 Trimethylamine/formic acid HCOO- PBE 94 17-0712-01 200 ml 4-9 115 cm/h
in the table below. For pre-packed columns see specific instructions supplied with
3.0-6.0 Pyridine/acetic acid CH3OO- Polybuffer exchanger
For most purifications, especially those where sample characteristics are unknown, Buffer concentration each product.
4.0-6.0 Trimethylamine/acetic acid CH3COO-
it is recommended to begin with a strong exchanger, allowing work over a broad 6.8-8.8 Trimethylamine/HCl Cl- PBE 118 17-0711-01 200 ml 8 - 11 115 cm/h
The buffer concentration should be sufficient to maintain buffering capacity and
pH range during initial method development. constant pH while an increasing salt concentration is applied. Recommended 7.0-8.5 Ammonia/formic acid HCOO- Purpose Procedure Polybuffer 74 17-0713-01 250 ml - -
8.5-10.0 Ammonia/acetic acid CH3COO- Removal of precipitated proteins 4 bed volumes of 0.5 - 1.0 M NaOH at 40 cm/h followed by 2 - 3 bed Polybuffer 96 17-0714-01 250 ml - -
Strong ion exchangers concentrations are shown in Tables 1 and 2. 7.0-12.0 Trimethylamine/CO2 CO3- volumes of water
Q (anionic), S or SP (cationic) are fully charged over a broad pH range. When working with a new sample of unknown charge characteristics, try these 7.0-12.0 Triethylamine/CO2 CO3-
Removal of strongly bound 4 - 10 bed volumes of up to 70% ethanol or 30% isopropanol
7.9 Ammonium bicarbonate HCO3-
conditions first: hydrophobic proteins, followed by 3 - 4 bed volumes of water
Weak ion exchangers 8.0-9.5 Ammonium carbonate/ammonia CO3-
lipoproteins and lipids or
DEAE, ANX (anionic) and CM (cationic) are fully charged over a narrower pH range Anion Exchange 8.5-10.5 Ethanolamine/HCl Cl-
1 - 2 bed volumes of 0.5% non-ionic detergent (e.g. in 1 M acetic acid)
(pH 2 - 9 and pH 6 - 10, respectively), but give alternative selectivities for separations. 8.9 Ammonium carbonate CO3-
Gradient: 0 - 100% elution buffer in 10 - 20 column volumes followed by 5 bed volumes of 70% ethanol to remove detergent,
Start buffer: 20 mM TrisTM-HCl pH 8 and 3 - 4 bed volumes of water
Media selection Elution buffer: 20 mM Tris-HCl + 1 M NaCl pH 8 (ÄKTATMexplorer and ÄKTApurifier chromatography systems give automatic advice Sanitization 0.5 - 1.0 M NaOH with a contact time of 30 - 60 min
on buffer recipes for each pH and media and the BufferPrep function compensates Sterilization Autoclave the media at 121°C for 15 min
Cation Exchange automatically for changes in pH caused by changes in salt concentration and
Parameters such as sample stability, scale of purification, speed of separation,
Gradient: 0 - 100% elution buffer in 10 - 20 column volumes temperature, ensuring constant pH throughout the separation.)
matrix binding capacity, resolution required and equipment available should be
Start buffer: 20 mM Na2HPO4 pH 6.8
considered when selecting a chromatographic medium.
Elution buffer: 20 mM Na2HPO4 + 1 NaCl pH 6.8
 Selection Guide - Ion Exchange Media A 280

MINI Q (PE 4.6/5) Sample: Pancreatin

Gradient elution
• extreme resolution

T his selection guide provides

general guidelines, based
on purification scale and
trace enrichment µg and less

mg and less
MiniBeads™ Q, S • micropurification and analysis
• upper-medium pressure systems
A 280
5.0 10.0 ml 15.0

Sample: Pancreatin
MONO Q (HR5/5)
sample conditions. • purification and analysis Gradient elution
MonoBeads™ Q, S
Analytical or • medium pressure systems
Additional information is semi-preparative mg and more
found in the Ion Exchange, 0 10 min 20 30

Principles and Methods, SOURCE 15 • high speed A 280

RESOURCE Q (1 ml) Sample: Pancreatin

Preparative • high capacity Gradient elution
Handbook available from separation Q, S
• ideal for scale up
Amersham Pharmacia Biotech. mg and more
• low-medium pressure systems
SOURCE 30
0 10 min 20 30

A 280

mg - kg SOURCE 30Q (XK16/5) Sample: Pancreatin

• lab / pilot scale separation of samples

Resolution
Gradient elution

Sepharose High Performance Q, SP • low-medium pressure systems

Partially purified or • clean up of small samples, use HiTrap columns
lab scale
0 10 min 20 30

A 280

Q Sepharose HP (XK16/5) Sample: Pancreatin

Capture and Gradient elution
process scale • fast separations of crude samples
• ideal for scale up
Sepharose Fast Flow Q, SP, DEAE, ANX, CM
• low-medium pressure systems
0 10 min 20 30

• method scouting, use HiTrap columns A 280

Q Sepharose FF (XK16/5) Sample: Pancreatin

Gradient elution
Selection of anion or cation exchangers high capacity and flow
Ion exchange separates biomolecules on the basis of differences in
charge characteristics (cationic/anionic).
• very high capacity to reduce manufacturing costs
Clarified Sepharose XL Q, SP
It is dependent on the pH of the buffer system and the isoelectric points • low-medium pressure systems
sample
of the biomolecules. 0

A 280
10 min 20 30

Q Sepharose XL Sample: Recombinant -amylase

The net charge of a protein as a function of pH Pilot scale: Gradient elution begins
Crude • initial capture of viscous samples after 20l
Will Bind feedstock Sepharose Big Beads Q, SP • low-medium pressure systems
Isoelectric point (pI)

Cation
Select Anion high viscosity
Exchanger
Exchange at System • industrial scale
pH Above pI Volume (l)
0 5.0 10.0 15.0 20.0

When buffer pH is above the pI, select an with particles / cell debris A 280

STREAMLINE SP Sample: Recombinant antigen

0 System pH anion (Q, DEAE, ANX) exchanger. binding fragment
4 6 8 10
Net Charge of Protein

pH • clarification, filtration, capture in one step Pilot scale: Step elution

Select Cation When buffer pH is below the pI, select a
Exchange at
System pH
cation (S, SP, CM) exchanger. STREAMLINE Q, DEAE, SP • low-medium pressure systems
Will Bind Below pI
Anion
The strong exchangers Q, S, SP maintain their charge over a wider pH range and are suitable for most applications. • industrial scale Sample application Washing, Buffer A Elution, Buffer B
Pool
Exchanger For media and pH scouting use HiTrap columns with automatic scouting on an ÄKTA chromatography system, or run 50 100 150 5 10 15
Volume (l)

on a low-medium pressure system.

Product Ordering Column dimensions Bed volume (ml) Recommended working Maximum Maximum operating pH working pH stability 3) pH stability 4) Antimicrobial treatment Type of ion exchanger Nominal bead Functional group Examples of dynamic capacities Applications
Information diam. (mm)/h(mm)/Pack size approx. flow rate range flow rate back pressure 1) (MPa/psi) range 2) (long term) (short term, cleaning) size (µm)
Prepacked columns/Bulk media Code No. (1 MPa = 10 bar)

Mini Q™ PC 3.2/3 17-0686-01 3.2/30 0.24 0.1 - 1.0 ml/min 1 ml/min 10/1450 3 - 11 3 - 11 1 - 14 sanitize strong anion 3 (monosized) -CH2N+(CH 3)3 a-amylase (Mr 49 000) 6 mg/ml gel extreme resolution, micropurification and analysis
Trypsin inhibitor (Mr 20 100) 6 mg/ml gel
Mini S™ PC 3.2/3 17-0687-01 3.2/30 0.24 0.1 - 1.0 ml/min 1 ml/min 10/1450 3 - 11 3 - 11 1 - 14 sanitize strong cation -CH2SO3- Ribonuclease (Mr 13 700) 5 mg/ml gel
Lysozyme (Mr 14 300) 5 mg/ml gel
Mini Q PE 4.6/50 17-5004-01 4.6/50 0.83 0.5 - 2.0 ml/min 2 ml/min 18/2600 3 - 11 3 - 11 1 - 14 sanitize strong anion -CH2N+(CH 3)3 a-amylase (Mr 49 000) 6 mg/ml gel
Trypsin inhibitor (Mr 20 100) 6 mg/ml gel
Mini S PE 4.6/50 17-5005-01 4.6/50 0.83 0.5 - 2.0 ml/min 2 ml/min 18/2600 3 - 11 3 - 11 1 - 14 sanitize strong cation -CH2SO3- Ribonuclease (Mr 13 700) 5 mg/ml gel
Lysozyme (Mr 14 300) 5 mg/ml gel
Mono Q™ PC 1.6/5 17-0671-01 1.6/50 0.1 0.01 - 0.4 ml/min 0.4 ml/min 5/711 2 - 12 2 - 12 2 - 14 sanitize strong anion 10 (monosized) -CH2N+(CH 3)3 Thyroglobulin (Mr 669 000) 25 mg/ml gel purification and analysis
Mono Q HR 5/5 17-0546-01 5/50 1 0.5 - 2.0 ml/min 2 m/min 5/711 HSA (M r 68 000) 65 mg/ml gel
Mono Q HR 10/10 17-0556-01 10/100 8 up to 6 ml/min 6 ml/min 5/711 a-lactalbumin (Mr 14 300) 80 mg/ml gel
Mono Q HR 16/10 17-0506-01 16/100 20 up to 10 ml/min 10 ml/min 3/427
Mono S™ PC 1.6/5 17-0672-01 1.6/50 0.1 0.01 - 0.4 ml/min 0.4 ml/min 5/711 2 - 12 2 - 12 2 - 14 sanitize strong cation 10 (monosized) -CH2SO3- IgG (human) (Mr 160 000) 75 mg/ml gel purification and analysis
Mono S HR 5/5 17-0547-01 5/50 1 0.5 - 2.0 ml/min 2 m/min 5/711 Ribonuclease (Mr 13 700) 75 mg/ml gel
Mono S HR 10/10 17-0557-01 10/100 8 up to 6 ml/min 6 ml/min 5/711
Mono S HR 16/10 17-0507-01 16/100 20 up to 10 ml/min 10 ml/min 3/427
RESOURCE™ Q 1 ml 17-1177-01 6.4/30 1 1.0 - 10 ml/min 10 ml/min 1.5/225 2 - 12 2 - 12 1 - 14 sanitize strong anion 15 (monosized) -CH2N+(CH 3)3 BSA (Mr 67 000) 45 mg/ml gel high speed, high capacity, ideal for scale up
RESOURCE Q 6 ml 17-1179-01 16/30 6 1.0 - 60 ml/min 60 ml/min 1.5/225
SOURCE™ 15Q PE 4.6/100 17-5065-01 4.6/100 1.7 0.5 - 2.5 ml/min 5 ml/min 4/580
RESOURCE S 1 ml 17-1178-01 6.4/30 1 1.0 - 10 ml/min 10 ml/min 1.5/225 2 - 12 2 - 12 1 - 14 sanitize strong cation 15 (monosized) -CH2SO3- Lysozyme (Mr 14 500) 80 mg/ml gel high speed, high capacity, ideal for scale up
RESOURCE S 6 ml 17-1180-01 16/30 6 1.0 - 60 ml/min 60 ml/min 1.5/225
SOURCE 15S PE 4.6/100 17-5067-01 4.6/100 1.7 0.5 - 2.5 ml/min 5 ml/min 4/580
SOURCE 15Q 17-0947-20 10 ml - 150 - 1800 cm/h 1800 cm/h 0.5/71 2 - 12 2 - 12 1 - 14 sanitize/sterilize strong anion 15 (monosized) -CH2N+(CH 3)3 BSA (Mr 67 000) 45 mg/ml gel high speed, high capacity, ideal for scale up
17-0947-01 50 ml
SOURCE 15S 17-0944-10 10 ml - 150 - 1800 cm/h 1800 cm/h 2 - 12 2 - 12 1 - 14 sanitize/sterilize strong cation 15 (monosized) -CH2SO3- Lysozyme (Mr 14 500) 80 mg/ml gel
17-0944-01 50 ml
SOURCE 30Q 17-1275-10 10 ml - 150 - 1000 cm/h 2000 cm/h 2 - 12 2 - 12 1 - 14 sanitize/sterilize strong anion 30 (monosized) -CH2N+(CH 3)3 HSA (M r 68 000) 50 mg/ml gel
17-1275-01 50 ml
SOURCE 30S 17-1273-20 10 ml - 150 - 1000 cm/h 2000 cm/h 2 - 12 2 - 12 1 - 14 sanitize/sterilize strong cation 30 (monosized) -CH2SO3- Lysozyme (Mr 14 500) 80 mg/ml gel
17-1273-01 50 ml
HiTrap Q Sepharose High Performance, 5 x 1 ml 17-1153-01 7/25 1 up to 1 ml/min 4 ml/min 0.3/42 2 - 12 2 - 12 2 - 14 sanitize strong anion 34 -CH2N+(CH 3)3 HSA (M r 68 000) 50 mg/ml gel small scale separation of sample, usable with a syringe, peristaltic pump or a
HiTrap Q Sepharose High Performance, 5 x 5 ml 17-1154-01 16/25 5 up to 5 ml/min 20 ml/min chromatographic system, high performance
HiTrap SP Sepharose High Performance, 5 x 1 ml 17-1151-01 7/25 1 up to 1 ml/min 4 ml/min 0.3/42 4 - 13 4 - 13 3 - 14 sanitize strong cation 34 -CH2CH2CH2SO3- Ribonuclease (Mr 13 700) 55 mg/ml gel small scale separation of sample, usable with a syringe, peristaltic pump or a
HiTrap SP Sepharose High Performance, 5 x 5 ml 17-1152-01 16/25 5 up to 5 ml/min 20 ml/min chromatographic system, high performance
HiLoad™ 16/10 Q Sepharose High Performance 17-1064-01 16/100 20 up to 5 ml/min 5 ml/min 0.3/42 2 - 12 2 - 12 1 - 14 sanitize strong anion 34 -CH2N+(CH 3)3 BSA (Mr 67 000) 60 mg/ml gel small / pilot scale separation of samples, high performance
HiLoad 26/10 Q Sepharose High Performance 17-1066-01 26/100 55 up to 13 ml/min 13 ml/min
HiLoad 16/10 SP Sepharose High Performance 17-1137-01 16/100 20 up to 5 ml/min 5 ml/min 0.3/42 4 - 13 4 - 13 3 - 14 sanitize strong cation 34 -CH2CH2CH2SO3- Ribonuclease (Mr 13 700) 55 mg/ml gel small / pilot scale separation of samples, high performance
HiLoad 26/10 SP Sepharose High Performance 17-1138-01 26/100 55 up to 13 ml/min 13 ml/min
Q Sepharose High Performance 17-1014-01 75 ml - up to 150 cm/h 150 cm/h 0.3/42 2 - 12 2 - 12 1 - 14 sanitize/sterilize strong anion 34 -CH2N+(CH 3)3 BSA (Mr 67 000) 70 mg/ml gel small / pilot scale separation of samples
SP Sepharose High Performance 17-1087-01 75 ml - up to 150 cm/h 150 cm/h 0.3/42 4 - 13 4 - 13 3 - 14 sanitize/sterilize strong cation 34 -CH2CH2CH2SO3 - Ribonuclease (Mr 13 700) 55 mg/ml gel
HiTrap IEX Selection Kit 5) 17-6002-33 7/25 1 up to 1 ml/min 4 ml/min 0.3/42 5) 5) 5) sanitize 5) 90 5) 5) ideal for method scouting and IEX media selection
HiTrap Q Sepharose Fast Flow, 5 x 1 ml 17-5053-01 7/25 1 up to 1 ml/min 4 ml/min 0.3/42 2 - 12 2 - 12 1 - 14 sanitize strong anion 90 -CH2N+(CH 3)3 HSA (M r 68 000) 120 mg/ml gel small scale fast separation of sample, ideal for scale up, usable with a syringe, a peristaltic pump or a
HiTrap Q Sepharose Fast Flow, 5 x 5 ml 17-5156-01 16/25 5 up to 5 ml/min 20 ml/min chromatographic system
HiTrap SP Sepharose Fast Flow, 5 x 1 ml 17-5054-01 7/25 1 up to 1 ml/min 4 ml/min 0.3/42 4 - 13 4 - 13 3 - 14 sanitize strong cation 90 -CH2CH2CH2SO3- Ribonuclease A (Mr 13 700) 70 mg/ml gel
HiTrap SP Sepharose Fast Flow, 5 x 5 ml 17-5157-01 16/25 5 up to 5 ml/min 20 ml/min
HiTrap DEAE Sepharose Fast Flow, 5 x 1 ml 17-5055-01 7/25 1 up to 1 ml/min 4 ml/min 0.3/42 2-9 2 - 12 1 - 14 sanitize weak anion 90 -CH2CH2N+H(CH2CH3)2 HSA (M r 68 000) 110 mg/ml gel
HiTrap DEAE Sepharose Fast Flow, 5 x 5 ml 17-5154-01 16/25 5 up to 5 ml/min 20 ml/min
HiTrap ANX Sepharose 4 Fast Flow (high sub), 5 x 1 ml 17-5162-01 7/25 1 up to 1 ml/min 4 ml/min 0.3/42 3 - 10 3 - 13 2 - 14 sanitize weak anion 90 -O-CH2CHOHCH2OCH2CHOHCH2N+H(CH2CH3)2 BSA (Mr 67 000) 43 mg/ml gel small scale fast separation of sample, ideal for scale up, particulary useful for separation of high
HiTrap ANX Sepharose 4 Fast Flow (high sub), 5 x 5 ml 17-5163-01 16/25 5 up to 5 ml/min 20 ml/min Thyroglobulin (Mr 669 000) 5 mg/ml gel molecular mass proteins. Usable with a syringe, a peristaltic pump or a chromatographic system
HiTrap CM Sepharose Fast Flow, 5 x 1 ml 17-5056-01 7/25 1 up to 1 ml/min 4 ml/min 0.3/42 6 - 10 4 - 13 2 - 14 sanitize weak cation 90 -O-CH2COO- Ribonuclease A (Mr 13 700) 50 mg/ml gel small scale fast separation of sample, ideal for scale up, usable with a syringe, a peristaltic pump or a
HiTrap CM Sepharose Fast Flow, 5 x 5 ml 17-5155-01 16/25 5 up to 5 ml/min 20 ml/min chromatographic system
HiLoad 16/10 Q Sepharose Fast Flow 17-1060-01 16/100 20 up to 7 ml/min 10 ml/min 0.3/42 2 - 12 2 - 12 1 - 14 sanitize strong anion 90 -CH2N+(CH 3)3 Thyroglobulin (Mr 669 000) 3 mg/ml gel fast separations of crude samples, ideal for scale up
HSA (M r 68 000) 120 mg/ml gel
HiLoad 26/10 Q Sepharose Fast Flow 17-1062-01 26/100 55 up to 18 ml/min 26 ml/min a-lactalbumin (Mr 14 300) 110 mg/ml gel
HiLoad 16/10 SP Sepharose Fast Flow 17-1135-01 16/100 20 up to 7 ml/min 10 ml/min 0.3/42 4 - 13 4 - 13 3 - 14 sanitize strong cation 90 -CH2CH2CH2SO3- IgG (human) (Mr 160 000) 50 mg/ml gel fast separations of crude samples, ideal for scale up
Bovine COHb (Mr 69 000) 50 mg/ml gel
HiLoad 26/10 SP Sepharose Fast Flow 17-1136-01 26/100 55 up to 18 ml/min 26 ml/min Ribonuclease (Mr 13 700) 70 mg/ml gel
HiPrep 16/10 CM 17-5091-01 16/100 20 2 - 10 ml/min 10 ml/min 0.3/42 6 - 10 4 - 13 2 - 14 sanitize weak cation 90 -O-CH2COO- Ribonuclease (Mr 13 700) 50 mg/ml gel fast separations of crude samples, ideal for scale up
HiPrep 16/10 DEAE 17-5090-01 16/100 20 2 - 10 ml/min 10 ml/min 0.3/42 2-9 2 - 13 1 - 14 sanitize weak anion -CH2CH2N+H (CH2CH3)2 a-lactalbumin (Mr 14 300) 100 mg/ml gel
Q Sepharose Fast Flow 17-0510-10 25 ml - 100 - 300 cm/h 750 cm/h 0.3/42 2 - 12 2 - 12 1 - 14 sanitize strong anion 90 -CH2N+(CH 3)3 Thyroglobulin (Mr 669 000) 3 mg/ml gel fast separations of crude samples, ideal for scale up
17-0510-01 300 ml HSA (M r 68 000) 120 mg/ml gel
a-lactalbumin (Mr 14 300) 110 mg/ml gel
SP Sepharose Fast Flow 17-0729-10 25 ml - 100 - 300 cm/h 750 cm/h 0.3/42 4 - 13 4 - 13 3 - 14 sanitize/sterilize strong cation 90 -CH2CH2CH2SO3- IgG (human) (Mr 160 000) 50 mg/ml gel
17-0729-01 300 ml Bovine COHb (Mr 69 000) 50 mg/ml gel
Ribonuclease (Mr 13 700) 70 mg/ml gel
DEAE Sepharose Fast Flow 17-0709-10 25 ml - 100 - 300 cm/h 750 cm/h 0.3/42 2-9 2 - 13 1 - 14 sanitize/sterilize weak anion 90 -CH2CH2N+H (CH2CH3)2 a-lactalbumin (Mr 14 300) 100 mg/ml gel
17-0709-01 500 ml HSA (M r 68 000) 110 mg/ml gel
ANX Sepharose 4 Fast Flow (high sub) 17-1287-10 25 ml - 100 - 300 cm/h 400 cm/h 0.1/14 3 - 10 3 - 13 2 - 14 sanitize/sterilize weak anion 90 -O-CH2CHOHCH2OCH2CHOHCH2N+H(CH2CH3)2 Thyroglobulin (Mr 669 000) 5 mg/ml gel fast separation of sample, ideal for scale up, particulary useful for separation of high molecular
17-1287-01 500 ml BSA (Mr 67 000) 43 mg/ml gel mass proteins
CM Sepharose Fast Flow 17-0719-10 25 ml - 100 - 300 cm/h 750 cm/h 0.3/42 6 - 10 4 - 13 2 - 14 sanitize/sterilize weak cation 90 -O-CH2COO- Ribonuclease (Mr 13 700) 50 mg/ml gel fast separations of crude samples, ideal for scale up
17-0719-01 500 ml
HiTrap Q XL, 5 x 1 ml 17-5158-01 7/25 1 up to 1 ml/min 4 ml/min 0.3/42 3 - 13 3 - 13 2 - 14 sanitize strong anion 90 -CH2N+(CH 3)3 BSA (Mr 67 000) >130 mg/ml gel small scale fast separation of sample, ideal for scale up, in certain applications very high loading
HiTrap Q XL, 5 x 5 ml 17-5159-01 16/25 5 up to 5 ml/min 20 ml/min capacity, usable with a syringe, a peristaltic pump or a chromatographic system
HiTrap SP XL, 5 x 1 ml 17-5160-01 7/25 1 up to 1 ml/min 4 ml/min 0.3/42 4 - 13 4 - 13 3 - 14 sanitize strong cation 90 -CH2CH2CH2SO3- Lysozyme (Mr 14 300) >160 mg /ml gel
HiTrap SP XL, 5 x 5 ml 17-5161-01 16/25 5 up to 5 ml/min 20 ml/min
HiPrep 16/10 Q XL 17-5092-01 16/100 20 2 - 10 ml/min 10 ml/min 0.3/42 3 - 13 3 - 13 2 - 14 sanitize strong anion 90 -CH2N+(CH 3)3 BSA (Mr 67 000) >130 mg/ml gel in certain applications very high capacity to reduce manufacturing costs
HiPrep 16/10 SP XL 17-5093-01 16/100 20 2 - 10 ml/min 10 ml/min 0.3/42 4 - 13 4 - 13 3 - 14 sanitize strong cation -CH2CH2CH2SO3- Lysozyme (Mr 14 500) >160 mg/ml gel
Q Sepharose XL on request - 100 - 500 cm/h 700 cm/h 0.3/42 3 - 13 3 - 13 2 - 14 sanitize strong anion 90 -CH2N+(CH 3)3 BSA (Mr 67 000) >130 mg/ml gel very high capacity to reduce manufacturing costs
SP Sepharose XL on request - 100 - 500 cm/h 700 cm/h 0.3/42 4 - 13 4 - 13 3 - 14 sanitize/sterilize strong cation -CH2CH2CH2SO3- Lysozyme (Mr 14 500) >160 mg/ml gel
Q Sepharose Big Beads on request - up to 300 cm/h 1800 cm/h 0.3/42 2 - 12 2 - 12 2 - 14 sanitize/sterilize strong anion 200 -CH2N+(CH 3)3 tested for specific application only initial capture of viscous samples, industrial scale
SP Sepharose Big Beads on request - up to 300 cm/h 1800 cm/h 0.3/42 4 - 13 4 - 13 3 - 14 sanitize/sterilize strong cation -CH2CH2CH2SO3- tested for specific application only
STREAMLINE DEAE on request - 200 - 400 cm/h expansion not applicable not applicable 2-9 2 - 13 2 - 14 sanitize weak anion 200 -CH2CH2N+H (CH2CH3)2 BSA (Mr 67 000) 55 mg/ml gel clarification, filtration, capture in one step, industrial scale, using expanded bed adsorption
STREAMLINE SP on request - 200 - 400 cm/h expansion 3 - 13 3 - 13 3 - 14 sanitize strong cation -CH2CH2CH2SO3- Lysozyme (Mr 14 500) 70 mg/ml gel
STREAMLINE Q XL on request - 300 - 500 cm/h expansion 2 - 12 2 - 12 2 - 14 sanitize strong anion -CH2N+(CH 3)3 BSA (Mr 67 000) >110 mg/ml gel
STREAMLINE SP XL on request - 300 - 500 cm/h expansion 4 - 13 4 - 13 3 - 14 sanitize strong cation -CH2CH2CH2SO3- Lysozyme (Mr 14 500) >140 mg/ml gel

1) Maximum operating back pressure refers to the pressure above which bed compression may begin. 5) HiTrap IEX Selection Kit includes: HiTrap Q Sepharose Fast Flow 1 ml, HiTrap SP Sepharose Fast Flow 1 ml, All ranges given are estimates based on our knowledge and experience. Maximum flow velocity (linear flow rate) is calculated from measurement in packed columns as follows:
2) Working pH range refers to the pH interval where the medium binds protein as intended or as needed for elution, without adverse long term effects. HiTrap DEAE Sepharose Fast Flow 1 ml, HiTrap CM Sepharose Fast Flow 1 ml, PC columns are designed for SMART™ System. Precision Column Holder (17-1455-01) is required for Sepharose Big Beads, ANX Sepharose 4 Fast Flow (high sub) XK50, bed height 25 cm
3) pH stability (long term) refers to the pH interval where the gel is stable over a long period of time without adverse effects on its subsequent chromatographic performance. HiTrap ANX Sepharose 4 Fast Flow (high sub) 1 ml, HiTrap Q XL 1 ml, HiTrap SP XL 1 ml. attachment to ÄKTApurifier and other HPLC systems Sepharose XL and Sepharose Fast Flow: XK50, bed height 15 cm
4) pH stability (cleaning) refers to the pH interval for regeneration, cleaning-in-place and sanitization procedures. cm/h: flow velocity (linear flow rate) = volumetric flow rate/column cross sectional area. Sepharose High Performance: BioPilot™ 60, bed height 30 cm
SOURCE 30: FineLINE™ 100, bed height 10 cm
SOURCE 15: RESOURCE, bed height 3 cm
Ordering Information

For more information about our Ion exchange

Columns Code No. Media Pack size Code No.

Mini Q PC 3.2/3* 17-0686-01 SOURCE 15Q 10 ml 17-0947-20

Mini S PC 3.2/3* 17-0687-01 50 ml 17-0947-01

chromatography columns and

Mini Q PE 4.6/50 17-5004-01 SOURCE 15S 10 ml 17-0944-10
Mini S PE 4.6/50 17-5005-01 50 ml 17-0944-01

columns and media

Mono Q PC 1.6/5* 17-0671-01 SOURCE 30Q 10 ml 17-1275-10
Mono Q HR 5/5 17-0546-01 50 ml 17-1275-01
Mono Q HR 10/10 17-0556-01 SOURCE 30S 10 ml 17-1273-20

media range, see our homepage:

Mono Q HR 16/10 17-0506-01 50 ml 17-1273-01
Mono S PC 1.6/5* 17-0672-01 Q Sepharose High Performance 75 ml 17-1014-01
Mono S HR 5/5 17-0547-01 SP Sepharose High Performance 75 ml 17-1087-01
Mono S HR 10/10 17-0557-01 Q Sepharose Fast Flow 25 ml 17-0510-10
Mono S HR 16/10 17-0507-01 300 ml 17-0510-01
RESOURCE Q 1 ml 17-1177-01 SP Sepharose Fast Flow 25 ml 17-0729-10
RESOURCE Q 6 ml 17-1179-01 300 ml 17-0729-01
SOURCE 15Q PE 4.6/100 17-5065-01 DEAE Sepharose Fast Flow 25 ml 17-0709-10
RESOURCE S 1 ml 17-1178-01 500 ml 17-0709-01
RESOURCE S 6 ml 17-1180-01 CM Sepharose Fast Flow 25 ml 17-0719-10
SOURCE 15S PE 4.6/100 17-5067-01 500 ml 17-0719-01

WWW.apbiotech.com
HiTrap Q Sepharose High Performance 5 x 1 ml 17-1153-01 ANX Sepharose 4 Fast Flow
HiTrap Q Sepharose High Performance 5 x 5 ml 17-1154-01 (high sub) 25 ml 17-1287-10
HiTrap SP Sepharose High Performance 5 x 1 ml 17-1151-01 500 ml 17-1287-01
HiTrap SP Sepharose High Performance 5 x 5 ml 17-1152-01 Q & SP Sepharose XL on request
HiLoad 16/10 Q Sepharose High Performance 17-1064-01 Q & SP Sepharose Big Beads on request
HiLoad 26/10 Q Sepharose High Performance 17-1066-01 STREAMLINE DEAE & SP on request
HiLoad 16/10 SP Sepharose High Performance 17-1065-01 STREAMLINE Q XL & SP XL on request
HiLoad 26/10 SP Sepharose High Performance 17-1137-01
Handbook:
HiLoad 26/10 SP Sepharose High Performance 17-1138-01
Ion Exchange Principles and Methods 18-1114-21
HiTrap IEX Selection Kit** 7 x 1 ml 17-6002-33
HiTrap Q Sepharose Fast Flow 5 x 1 ml 17-5053-01
HiTrap Q Sepharose Fast Flow 5 x 5 ml 17-5156-01 Larger pack sizes for media are available on request.
HiTrap SP Sepharose Fast Flow 5 x 1 ml 17-5054-01
HiTrap SP Sepharose Fast Flow 5 x 5 ml 17-5157-01 *PC colums are designed for SMART System. Precision Column
HiTrap DEAE Sepharose Fast Flow 5 x 1 ml 17-5055-01 Holder (17-1455-01) is required for attachment to ÄKTApurifier
HiTrap DEAE Sepharose Fast Flow 5 x 5 ml 17-5154-01 and other HPLC systems.
HiTrap CM Sepharose Fast Flow 5 x 1 ml 17-5056-01 **HiTrap IEX Selection Kit: Q Sepharose Fast Flow, DEAE Sepharose
HiTrap CM Sepharose Fast Flow 5 x 5 ml 17-5155-01 Fast Flow, SP Sepharose Fast Flow, CM Sepharose Fast Flow,
HiTrap ANX Sepharose 4 Fast Flow (high sub) 5 x 1 ml 17-5162-01 ANX Sepharose 4 Fast Flow (high sub), Q Sepharose XL and
HiTrap ANX Sepharose 4 Fast Flow (high sub) 5 x 5 ml 17-5163-01 SP Sepharose XL pre-packed in 1 ml HiTrap columns.
HiTrap Q XL 5 x 1 ml 17-5158-01
HiTrap Q XL 5 x 5 ml 17-5159-01
HiTrap SP XL 5 x 1 ml 17-5160-01
HiTrap SP XL 5 x 5 ml 17-5161-01
HiPrep 16/10 Q XL 17-5092-01
HiPrep 16/10 SP XL 17-5093-01 ÄKTA, SMART, HiTrap, HiLoad, Sepharose, Sephadex, Mono S, Mono Q,
HiPrep 16/10 CM 17-5091-01 Mono P, MiniBeads, MonoBeads, Mini S, Mini Q, RESOURCE, BioProcess,
HiPrep 16/10 DEAE 17-5090-01 STREAMLINE, SOURCE, BioPilot, FineLINE, Polybuffer and HiPrep are
HiLoad 16/10 Q Sepharose Fast Flow 17-1060-01 trademarks of Amersham Pharmacia Biotech Limited or its subsidiaries
HiLoad 26/10 Q Sepharose Fast Flow 17-1062-01
Amersham is a trademark of Nycomed Amersham plc
HiLoad 16/10 SP Sepharose Fast Flow 17-1135-01
Pharmacia and Drop Design are trademarks of Pharmacia & Upjohn Inc
HiLoad 26/10 SP Sepharose Fast Flow 17-1136-01
Tris is a trademark of Union Carbide Chemical and Plastics Co. Selection guide and product profile
All goods and services are sold subject to the terms and conditions of sale of the
BioProcess company within the Amersham Pharmacia Biotech group which supplies them.
A copy of these terms and conditions is available on request.
Media

BioProcess Media - Media for bioprocessing

Secure Supply
Validated Manufacture
Regulatory Support
Large capacity production integrated with clear ordering and delivery routines means © Amersham Pharmacia Biotech UK Limited 2000 - All rights reserved
BioProcess™ media are available in the right quantity, at the right place, at the right time.
We can assure future supplies of BioProcess Media, making them a safe investment for Amersham Pharmacia Biotech UK Limited Amersham Place Little Chalfont
your long term production. Buckinghamshire England HP7 9NA
Amersham Pharmacia Biotech AB SE-751 84 Uppsala Sweden
Produced following validated methods and tested under strict control, BioProcess Media fulfill high performance specifications.
A certificate of analysis is available with each order. Amersham Pharmacia Biotech Inc 800 Centennial Avenue PO Box 1327
Piscataway NJ 08855 USA
Regulatory Support Files contain details of performance, stability, extractable compounds and analytical methods available.
Amersham Pharmacia Biotech Europe GmbH Munzinger Strasse 9,
The essential information in these files is an invaluable starting point for process validation, as well as support for clinical and
marketing applications submitted to regulatory authorities. D-79111 Freiburg, Germany

From Capture to Polishing
High Productivity
Sanitization/CIP
Scalability
Specific BioProcess Media have been designed for each chromatographic stage in a process from Capture to Polishing.
Using BioProcess Media for every stage results in an easily validated economic process.
High flow rates, high capacity and high recovery contribute to the overall economy of an industrial process.
All BioProcess Media have high chemical stability to allow efficient cleaning and sanitization procedures.
Packing methods are established for a wide range of scales and compatible large scale columns and equipment are available. 18-1127-31
Edition AC
Ordering Information

For more information about our Ion exchange

Columns Code No. Media Pack size Code No.

Mini Q PC 3.2/3* 17-0686-01 SOURCE 15Q 10 ml 17-0947-20

Mini S PC 3.2/3* 17-0687-01 50 ml 17-0947-01

chromatography columns and

Mini Q PE 4.6/50 17-5004-01 SOURCE 15S 10 ml 17-0944-10
Mini S PE 4.6/50 17-5005-01 50 ml 17-0944-01

columns and media

Mono Q PC 1.6/5* 17-0671-01 SOURCE 30Q 10 ml 17-1275-10
Mono Q HR 5/5 17-0546-01 50 ml 17-1275-01
Mono Q HR 10/10 17-0556-01 SOURCE 30S 10 ml 17-1273-20

media range, see our homepage:

Mono Q HR 16/10 17-0506-01 50 ml 17-1273-01
Mono S PC 1.6/5* 17-0672-01 Q Sepharose High Performance 75 ml 17-1014-01
Mono S HR 5/5 17-0547-01 SP Sepharose High Performance 75 ml 17-1087-01
Mono S HR 10/10 17-0557-01 Q Sepharose Fast Flow 25 ml 17-0510-10
Mono S HR 16/10 17-0507-01 300 ml 17-0510-01
RESOURCE Q 1 ml 17-1177-01 SP Sepharose Fast Flow 25 ml 17-0729-10
RESOURCE Q 6 ml 17-1179-01 300 ml 17-0729-01
SOURCE 15Q PE 4.6/100 17-5065-01 DEAE Sepharose Fast Flow 25 ml 17-0709-10
RESOURCE S 1 ml 17-1178-01 500 ml 17-0709-01
RESOURCE S 6 ml 17-1180-01 CM Sepharose Fast Flow 25 ml 17-0719-10
SOURCE 15S PE 4.6/100 17-5067-01 500 ml 17-0719-01

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HiTrap Q Sepharose High Performance 5 x 1 ml 17-1153-01 ANX Sepharose 4 Fast Flow
HiTrap Q Sepharose High Performance 5 x 5 ml 17-1154-01 (high sub) 25 ml 17-1287-10
HiTrap SP Sepharose High Performance 5 x 1 ml 17-1151-01 500 ml 17-1287-01
HiTrap SP Sepharose High Performance 5 x 5 ml 17-1152-01 Q & SP Sepharose XL on request
HiLoad 16/10 Q Sepharose High Performance 17-1064-01 Q & SP Sepharose Big Beads on request
HiLoad 26/10 Q Sepharose High Performance 17-1066-01 STREAMLINE DEAE & SP on request
HiLoad 16/10 SP Sepharose High Performance 17-1065-01 STREAMLINE Q XL & SP XL on request
HiLoad 26/10 SP Sepharose High Performance 17-1137-01
Handbook:
HiLoad 26/10 SP Sepharose High Performance 17-1138-01
Ion Exchange Principles and Methods 18-1114-21
HiTrap IEX Selection Kit** 7 x 1 ml 17-6002-33
HiTrap Q Sepharose Fast Flow 5 x 1 ml 17-5053-01
HiTrap Q Sepharose Fast Flow 5 x 5 ml 17-5156-01 Larger pack sizes for media are available on request.
HiTrap SP Sepharose Fast Flow 5 x 1 ml 17-5054-01
HiTrap SP Sepharose Fast Flow 5 x 5 ml 17-5157-01 *PC colums are designed for SMART System. Precision Column
HiTrap DEAE Sepharose Fast Flow 5 x 1 ml 17-5055-01 Holder (17-1455-01) is required for attachment to ÄKTApurifier
HiTrap DEAE Sepharose Fast Flow 5 x 5 ml 17-5154-01 and other HPLC systems.
HiTrap CM Sepharose Fast Flow 5 x 1 ml 17-5056-01 **HiTrap IEX Selection Kit: Q Sepharose Fast Flow, DEAE Sepharose
HiTrap CM Sepharose Fast Flow 5 x 5 ml 17-5155-01 Fast Flow, SP Sepharose Fast Flow, CM Sepharose Fast Flow,
HiTrap ANX Sepharose 4 Fast Flow (high sub) 5 x 1 ml 17-5162-01 ANX Sepharose 4 Fast Flow (high sub), Q Sepharose XL and
HiTrap ANX Sepharose 4 Fast Flow (high sub) 5 x 5 ml 17-5163-01 SP Sepharose XL pre-packed in 1 ml HiTrap columns.
HiTrap Q XL 5 x 1 ml 17-5158-01
HiTrap Q XL 5 x 5 ml 17-5159-01
HiTrap SP XL 5 x 1 ml 17-5160-01
HiTrap SP XL 5 x 5 ml 17-5161-01
HiPrep 16/10 Q XL 17-5092-01
HiPrep 16/10 SP XL 17-5093-01 ÄKTA, SMART, HiTrap, HiLoad, Sepharose, Sephadex, Mono S, Mono Q,
HiPrep 16/10 CM 17-5091-01 Mono P, MiniBeads, MonoBeads, Mini S, Mini Q, RESOURCE, BioProcess,
HiPrep 16/10 DEAE 17-5090-01 STREAMLINE, SOURCE, BioPilot, FineLINE, Polybuffer and HiPrep are
HiLoad 16/10 Q Sepharose Fast Flow 17-1060-01 trademarks of Amersham Pharmacia Biotech Limited or its subsidiaries
HiLoad 26/10 Q Sepharose Fast Flow 17-1062-01
Amersham is a trademark of Nycomed Amersham plc
HiLoad 16/10 SP Sepharose Fast Flow 17-1135-01
Pharmacia and Drop Design are trademarks of Pharmacia & Upjohn Inc
HiLoad 26/10 SP Sepharose Fast Flow 17-1136-01
Tris is a trademark of Union Carbide Chemical and Plastics Co. Selection guide and product profile
All goods and services are sold subject to the terms and conditions of sale of the
BioProcess company within the Amersham Pharmacia Biotech group which supplies them.
A copy of these terms and conditions is available on request.
Media

BioProcess Media - Media for bioprocessing

Secure Supply
Validated Manufacture
Regulatory Support
Large capacity production integrated with clear ordering and delivery routines means © Amersham Pharmacia Biotech UK Limited 2000 - All rights reserved
BioProcess™ media are available in the right quantity, at the right place, at the right time.
We can assure future supplies of BioProcess Media, making them a safe investment for Amersham Pharmacia Biotech UK Limited Amersham Place Little Chalfont
your long term production. Buckinghamshire England HP7 9NA
Amersham Pharmacia Biotech AB SE-751 84 Uppsala Sweden
Produced following validated methods and tested under strict control, BioProcess Media fulfill high performance specifications.
A certificate of analysis is available with each order. Amersham Pharmacia Biotech Inc 800 Centennial Avenue PO Box 1327
Piscataway NJ 08855 USA
Regulatory Support Files contain details of performance, stability, extractable compounds and analytical methods available.
Amersham Pharmacia Biotech Europe GmbH Munzinger Strasse 9,
The essential information in these files is an invaluable starting point for process validation, as well as support for clinical and
marketing applications submitted to regulatory authorities. D-79111 Freiburg, Germany

From Capture to Polishing
High Productivity
Sanitization/CIP
Scalability
Specific BioProcess Media have been designed for each chromatographic stage in a process from Capture to Polishing.
Using BioProcess Media for every stage results in an easily validated economic process.
High flow rates, high capacity and high recovery contribute to the overall economy of an industrial process.
All BioProcess Media have high chemical stability to allow efficient cleaning and sanitization procedures.
Packing methods are established for a wide range of scales and compatible large scale columns and equipment are available. 18-1127-31
Edition AC