pBR322
© 2013 Elsevier Inc. All rights reserved.
This article is reproduced from the previous edition, volume 3, p 1428, © 2001, Elsevier Inc., with revisions made by the Editor.
pBR322 is a double-stranded, circular, 4361 base pair plasmid See also: Compatibility Group; Plasmids.
cloning vector (Figure 1). pBR322 was the first artificial plasmid,
containing the ampR gene (encoding ampicillin resistance)
derived from plasmid RSF2124, the tetR gene (encoding tetracy Further Reading
cline resistance) derived from plasmid pSC101, and the origin of
replication from pMB1 (a plasmid in the ColE1 compatibility Balbás P and Bolívar F (2004) Back to basics: pBR322 and protein expression systems in
group). pBR322 also encodes the rop gene product, which reg E. coli. Methods in Molecular Biology 267: 77–90.
ulates plasmid replication by stabilizing the interaction between Balbas P, Soberon X, Bolivar F, and Rodriguez RL (1988) The plasmid, pBR322.
Biotechnology 10: 5–41.
RNAI and RNAII transcripts, and thereby maintains the plasmid
at ∼20 copies per bacterium. To facilitate its use as a cloning
vector, pBR322 was constructed to have unique sites for a variety
of useful restriction endonucleases. pBR322 was created by Relevant Websites
Bolivar and Rodriguez in 1977, where p stands for plasmid, B
for Bolivar, and R for Rodriguez. pBR322 was one of the most http://ecoliwiki.net – EcoliWiki; pBR322.
widely used plasmid cloning vectors. The complete DNA http://www.neb.com – New England BioLabs Inc.; pBR322 Vector.
sequence is available via GenBank (Accession number: J01749). http://genome-www.stanford.edu/vectordb/vector_descrip/PBR322.html – pBR322.
Cla I - BspD I 23
EcoR I - Apo I 4359 Hind III 29
Aat II 4284 EcoR V 185
BclV I 4209
BsrB I 4205 Nhe I 229
Ssp I 4168 BamH I 375
Ear I 4155 SgrA I 409
Eco57 I 4048 Ban II 471
Ban II 485
Hine II 3905 Xmn I 3961 Btg I 528
Sca I 3844 Sph I 562
Bts I 3787 EcoN I 622
Bts I 3759 Sal I 651
pυu I 3733 Hine II 651
pst I 3607 Acc I 651
BsrD I 3602
Tc
Ap
PshA I 712
Ase I 3537
Bsa I 3433 Eag I 939
BsrD I 3420 Nru I 972
Ahd I 3361 BstAP I 1045
BspM I 1063
PfIM I 1315
Eco57 I 3000 Bsm I 1353
Sty I 1369
or
PflM I 1364
i
AlωN I 2884 Aυa I-BsoB I 1425
PpuM I 1438
BclV I 2682 Msc I 1444
Drd I 2575 Btg I 1447
rop PpuM I 1480
Afl III-Pci I 2473
BsrB I 2404 Bpu10 I 1580
Ear I 2351 Xmn I 2029 Bsg I 1650
Sap I 2350 Pυu II 2064 BspE I 1664
Nde I 2295 BsmB I 2122 BsaB I 1668
BstAP I 2291 Drd I 2162
BstZ17 I - Acc I 2244
BsaA I 2225 PfIFI - Tth111 I 2217
Figure 1 A physical map of plasmid pBR322 (via New England Biolabs).
Brenner’s Encyclopedia of Genetics, 2nd edition, Volume 5 doi:10.1016/B978-0-12-374984-0.01129-3 249
