7.75058.0004-xxxxxxxxxx msp.
September 2020
1.14418.0001 7. Procedure
Open the box and set up with both test tubes on the left.
MQuant® Slide the comparator block all the way to the left, so that the end holding the
Copper Test Cu test tubes protrudes laterally over the bottom part of the box.
Unfold the color card and insert it, colored end first, into the slit at the lower
right-hand edge of the box.
Measurement Blank
sample
1. Method tube nearer to tube farther from
the tester (A) the tester (B)
Determination with color-card comparator
In an ammoniacal medium copper(II) ions react with cuprizone to form a Pretreated 5 ml 5 ml Inject into the test
blue complex. The copper concentration is measured semiquantitatively sample tube with the syringe.
by visual comparison of the color of the measurement solution with the color (10 - 30 °C)
fields of a color card. Reagent Cu-1 1 level orange- - Add, close the tube,
colored micro- and shake vigorously
2. Measuring range and number of determinations spoon (in the until the reagent is
cap of the Cu-1 completely dissolved.
bottle) The pH must be
Measuring range / color-scale Number of within the range
graduation determinations 7.0 - 9.5.
Check with MQuant®
0.3 - 0.6 - 1.0 - 1.5 - 2.0 - 2.5 - 3 - 4 - 5 mg/l Cu 125
pH-indicator strips.
Reagent Cu-2 5 drops 1) - Add, close the tube,
3. Applications and mix.
This test measures only copper(II) ions. When hydrogen peroxide is Leave to stand for 5 min (reaction time).
added, copper(I) ions are measured at the same time (see section 6).
Slide the color card through to the left until the closest possible colour match is
Sample material: achieved between the two open test tubes when viewed from above.
Groundwater, surface water, and seawater
Read off the result in mg/l Cu from the color card at the lower right-hand edge
Drinking water of the comparator block within the bottom part of the box.
Wastewater and percolating water
Boiler and boiler feed water, cooling water Hold the bottle vertically while adding the reagent!
1)
Electroplating wastewater
Food after appropriate sample pretreatment Notes on the measurement:
Swimming-pool water
• The color of the measurement solution remains stable for at least
30 min after the end of the reaction time stated above. (After 45 min
4. Influence of foreign substances the measurement value would have diminished by 5 %.)
This was checked individually in solutions containing 2.5 and 0 mg/l Cu. • Turbidity in the measurement solution makes the color comparison more
The determination is not yet interfered with up to the concentrations of difficult.
foreign substances given in the table. Cumulative effects were not checked;
such effects can, however, not be excluded. • If the color of the measurement solution is equal to or more intense than
the darkest color on the scale, repeat the measurement using fresh,
Concentrations of foreign substances in mg/l or % diluted samples until a value of less than 5 mg/l Cu is obtained.
• In the event of copper concentrations exceeding 50 mg/l, other reaction
Ag +
100 F- 1000 Pb2+ 100 Ascorbic acid 10
Al3+ 1000 Fe3+ 100 PO43- 1000 EDTA 5 products are formed and false-low readings are yielded. In such cases it
Ca2+ 100 Hg2+ 100 S2- 0,1 Hydrazine 10 is advisable to conduct a plausibility check of the measurement results
Cd2+ 100 Mg2+ 1000 SCN- 1000 Surfactants 1) 5 % by diluting the sample (1:10, 1:100).
CN- 0,1 Mn2+ 1000 SiO32- 100 Na-acetate 20 % • Concerning the result of the analysis, the dilution must be taken into
CO32- 1000 NH4
+
1000 Sn
2+
100 NaCl 15 %
Cr3+ 10 Ni2+ 100 SO32- 1000 NaNO3 15 % account:
Cr2O72- 100 NO2- 1000 Zn2+ 100 Na2SO4 15 %
Result of analysis = measurement value x dilution factor
tested with nonionic, cationic, and anionic surfactants
1)
5. Reagents and auxiliaries 8. Method control
To check test reagents, measurement device, and handling:
The test reagents are stable up to the date stated on the pack when Dilute the copper standard solution with distilled water to 2.5 mg/l Cu and
stored closed at +15 to +25 °C. analyze as described in section 7.
Additional notes see under www.qa-test-kits.com.
Package contents:
4 bottles of reagent Cu-1 9. Notes
1 bottle of reagent Cu-2
1 graduated 5-ml plastic syringe • Reclose the reagent bottles immediately after use.
2 test tubes with screw caps (in comparator block) • Rinse the test tubes with distilled water only.
1 color card • Information on disposal can be obtained at
Other reagents and accessories: www.disposal-test-kits.com.
Nitric acid 65 % for analysis EMSURE®, Cat. No. 100456
Hydrogen peroxide 30 % H2O2 (Perhydrol®) for analysis EMSURE®,
Cat. No. 107209
MQuant® Universal indicator strips pH 0 - 14, Cat. No. 109535
MQuant® pH-indicator strips pH 5.0 - 10.0, Cat. No. 109533
Sodium hydroxide solution 1 mol/l Titripur®, Cat. No. 109137
Sulfuric acid 0.5 mol/l Titripur®, Cat. No. 109072
Copper standard solution Certipur®, 1000 mg/l Cu, Cat. No. 119786
Refill pack:
Cat. No. 118459
Copper Test
Refill pack for 114765, 114418, and 114414
(Reagents without technical accessories for the number of determina-
tions stated in section 2)
6. Preparation
• Analyze immediately after sampling. Otherwise preserve with nitric acid
65 % (1 ml nitric acid per 1 l of sample solution).
• To determine copper(I) ions add a few drops of Perhydrol to the sample
®
Merck KGaA, 64271 Darmstadt, Germany,
and mix. Tel. +49(0)6151 72-2440
www.sigmaaldrich.com/mobile-laboratory
• The pH must be within the range 4 - 10. EMD Millipore Corporation, 400 Summit Drive
Adjust, if necessary, with sodium hydroxide solution or sulfuric acid. Burlington MA 01803, USA, Tel. +1-978-715-4321
Sigma-Aldrich Canada Co. or Millipore (Canada) Ltd.
• Filter turbid samples. 2149 Winston Park, Dr. Oakville, Ontario, L6H 6J8
Phone: +1 800-565-1400
