ne ssiguument — 4 |what i$ GLC explain itS principle and method Ccayvied out? fas In gas-liquid Chromatography CGL CG), the mobile phase 1S a gas ana the Stationayy phase iS & liquid immoblized on the Suvface of an inevt solid suppo7t. ly The technique is based 02 the diffevential Partioning of the ‘omponents of a Sample in [the vapouy phase between the mobile gas Phasé Q@nd the statronavy liqauicd phase, This in tuyvn causes Q afffeventral Migration of the ‘fomponents, Theveby effecting Theiy Sepe yaton, x» The liquid phase iS genevallg a non-volatile liquich Uke Methdgs Silitone Or Potgethgiene | Gg. : . | » Ms is efthey Supported ego smat | 7 i . particles of an mere Poros soli Uke | euatomaceouys etuth , whith the. coluen iS calleh Packed t0lamn esuaily 1-10 meters Long aa @-G mm i4 innner deameterFEES EPR ag eS eee eee eee eee tk Ov Lt i$ loatcd on a cappillary wall of Chemically mocttied glass oy fused Silita ; the ) tolamn wis - Calleckh Q Ccappllerg Column (ov open tubular column) 4ancis normally 15-100 metres tong and O)-0% avametey, mm in internal contained in a coiled + Both cwes being column, * The mobile phase, also catled the Carviev gas since Its majoy function is & trankport the analytes through the folamn, 6S &@ pire Che méicaily ihert Gy Suth ag helium, argon, rLtvogen ov CO, inter val betwen (a Retention times :- Time sample intvoduchHon and The Ve tortlings of diffevent peaks. > allow quantitate identification of the components, ly Peak areas Quantitative Cet edmiination | | The pvotess | panne The Sthemeatic weprecent ation of The + basic components of a GALS instvumac | Calledth gas Chaomatograpn Is | shown in The Figune.Synge Flowmeter: Pressure vequia tov Elow i MM ae Amplifier Rewvdey. Detector. Larmier gas Th evmostated ews cylinder, suppte ect into the a The Carvvicv gas is apparatus from a pressurize Cylinder. The gas passes through 4 + cug- Stage atov and flow Contrdley. is TULMGL pore + pressure wegul 4+ A Ssmatt amount of the Vapioly injecteek bY means of & SY into a heated thamper througn + ulich iS coverech by & wabbey septum. ¥ micro sgvinge > Le Gaedk GU- tigne | gas sampling value ga. Coltmn by Sample + Sample is corntec onto mobile pnoe. 1 S?pevation of 6 ther differential partiomeg anit 6 Staten lunia 9g. omponents orcs clue tO egutlibinum between th liquid phase and theComponents cucttementinily vetamedia the stutionarg puure veal, The foluny outler ae otforent times Eath component that lomesout rs wmeced ately cotecbead tive Ottechorv, ¥ ? of the | lolumn anromestrcadig by a sens ¥ TCD g FID are Mose Commonly | aed genet lek ectors emer genre of eath Compone colum fs ctotedet. the catcctov response rs seal T0:a vecovrdev wluch ylelcls a plot of Snegnal ty as a Ftunction of time. Ns soon, & The by nt dsvom the fnkengi A compute ottatet to We sysirm can csp! on i6§ monctov the Above Qriefebircal fornrt Of The Chromuto: vup Ute wun Or Can proklwue a prvtout of the Same, x K——._ Retention time fx ————— Time nd > PK—— Retention » time for Y 2 S Carview g a u * ——Anca Unter « peak = peak hecgut x peck ucAth at hey, Megat. 9, of A Component = Aven uncda the emponent peck oo Totet aren 2.| Discuss the prcuueple afd techircque of g nig ree! adsorption and Column Carometograprg? Ack sorption Cavomatograp hy: In thes ty pe, the wetardation mechanism thar ectublisnes ditlevential nugraton of Sample components in theiv Selective walsoption on & souk adsovb nat Uqueu— Sottn Caronnnlograpug (ES) and gas -sored chvomatograp ny COSt) cate gory, eat. Conventio- Qe sub ceewesion8 of This prvinumple of act sorp H0- column Choo maato grep neg Ack Sovb tion Chvomatograpry ov guid ZauUcd adsorb tio Cavomcntogva peg cs baget on “the Selecerve AUSO7)/> ti OF jthe sample components on an actsorbent folenn Like sileceb gee alumina ov Cellatose uhith Constitutes The ¢tectloncay plConsists of & Suitable x The mobile pnase | Solvent like petvoleam ether, qcetonc, benzene etc. y Each Components tends te equilibrte between the ftiwo phases. ly Gach is, however ander the taFluene of two opposing forces 1a vetavhing forte Qnuv a ariving Forte. eC bc! vesule 1s 3a cut evential ot the Components along / maugvation stattonary pha | the column of the effecting Sepey action, ¢ The Component most weakly acksoied emerges Oak of the Columm Vise and most Sees) adsorbeh Comes out (ase. : The process x An acl govbent column I$ preperecl by | PS a lon wAs. +ube cunitornd ee “. sactuble adclvovbent. Pp A soloction oF sate nuixtusrce OF the components in a Suutabele Solvent (Ss then intrvotuted at the to of the column, » Mos weacttly Arsovbet Gonsatituent Cea A) 6 helt athe Tp+ The owthess with checrectsi ng geovbabl ities ane held tf tn cluittAent Zonev ov bands le This particl seperation “ray be lomprovech bY passing some more of The original solvent ov some other Suitable Solvent. This process js known af the clevelopment of the. Chvometogr7am. lL The inciuccduel components me extracted fvom the adsorbent by Passi” aA | Cuttable Solvent. The Components Me then | washek clown one by one in The increasing ovdev of thelv adsorbabilities | and cue collecked in Seperate fraction This process 16 canen Clutton and thé | Solvent wreck for The Purpose TS callec! elubent. [Dis ting aise between pap mr and Hhin chromatogrep | paper chrom szogr apres (PO lls @ spect field of wqued- Liquid [Pwrtietion Cho mune gVophy - lu thes Hechucqget ; the stationary phone is & film of L0q Kd (e9 wetrt) aclsoreed on aA pope moat cn eng mace PF htguly Puritieh celudose. the mobile piuase ts Qnotur Mquck Perloluting ovum re= ee lees equi libration of