rose Spcaation Teaning of Graces 7-0 Scitce Teachers, Grade 10 Activity Sheet on Protein Synthesie Activity 3: Decoding DNA segment Objective ‘Apply the concept of protein synthesis through the amino acids coded for the ‘mRNA codon using the genetic code. Materials Pen Crayons Activity sheet: Decoding DNA segment Procedure 1. Study the glven scenario (see attached activity] and study the profile of the suspects and adaitional information, 2. Using your knowledge about DNA and RNA base-pairing, decode the DNA sample in ‘order to identify the sequence of amino acids, Use the amino acid identifier chart as your reference 2. Compare the sequence of amino acids (proteins) to the profile of the suspects and ‘additional information given in order to determine the corresponding traits 4. Name the culprit using the dete or information you got 5. Using the pencil and crayons make a cartographic sketch of the suspect. 11. What amino acids sequences did you get upon decoding the genes? nose hair shape of eyes lips ear attachment: type of hair 2. What are the traits of the culprit based on your data? 03. Who among the suspects matches with the sequence of amino acids (proteins) you got? (Q4. Drew the possible appearance of the culprit.Decoding DNA Segment An intruder rozed the science laboratory of « Junior High School. The school is in choos. The schoo! administrators ond teachers demanded fo know the culpt They have to Identify the culprit and do it fest. They had three suspects namely Rudy Tee, Von Go and Rambo Tan, As a forensic expert, your mission Is 10 Identity the intruder using the profile af the suspects ang otnar information taken From tna laboratory: THE PROFILE OF THE SUSPECTS Rudy + Funked physics class three + tulips times + atiached earlobe + with ong nose hat. + shoighthar + ovaleyes Von Go «His investigative project is «short nose hair about the potential «© chinky eyes applications of nuclear * fullips fusion in generating + free earlobe energy. + curly hair Rambo Tan + Suspended for a week by the Disciplinary Committee for repeated tardiness. + short nose hair oval eyes haretip attached earlobe © wavy hairAdditional Information: Below are the specific amino acid sequence for specific trait 1. Nose hair © Short: phe-tys + Long: eu-lys 2. Shape of Eyes oval: varola-ala © chinky : vakoleval 3, Lips © Fulllips :phe-glu-ola + Horelip : phe-lys-ala 4, Ear attachment + Free earlobe : thre tyrser * Attached : Jeu-arg-aly 5. Type of hair © Straight :Iys-alu + Wavy: phe-aie = Curly gu-phe THE DNA CODE OF THE SUSPECT Alfresh DNA sample was obtained rom the laboratory; believe to have been left by the suspect. Using the DNA sequencer the following nucleotide sequence was obtained: SUSPECT: _TAC-AAAAIT-ATC-TAC-AAA-CTT-CGT-AIC-TAC-CAT-CGT-CAPATETAC-IGG- ATATCG-AIC-TAC-CT-ASAATC ‘Complementary base pairs: DNA Is represented by niirogen bases that are read in groups of threes. To decode, use the DNA as a blueprint to procuce mRNA Then use the MRNA as blueprint to produce the lingar sequence of IRNA. A specific nitroge base in the DNA pairs up with a specific nitrogen base in the mRNA, and ¢ specific nitrogen base In the mRNA pairs up with a specific niirogen base In the 1RNA. Breaking the Code: Complete the data table below.DNA triplet code TAC ‘mRNA FRNA ‘Amino acid BAA 11 AC TAC BAA or CGT AIC TAC CAT CGT CAT AIT TAC TGS ATA ToS AIC TAC or AAA, AIG Characteristics and traits of the suspect based on the DNA code. 1. Nose heir: 2. Byes: 3. Lips: 4, Ear attachment: 5. Type of har Name and cortographic sketch of the suspect