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Nanopore DNA Sequencing Explained

Nanopore sequencing is a method for determining the order of nucleotides in DNA, revealing the genetic information essential for organisms. It employs tiny nanopores, either biological or solid-state, to sequence DNA by measuring changes in current as nucleotides pass through. This technique aids in understanding genetic traits, diseases, and evolutionary relationships.

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13 views6 pages

Nanopore DNA Sequencing Explained

Nanopore sequencing is a method for determining the order of nucleotides in DNA, revealing the genetic information essential for organisms. It employs tiny nanopores, either biological or solid-state, to sequence DNA by measuring changes in current as nucleotides pass through. This technique aids in understanding genetic traits, diseases, and evolutionary relationships.

Uploaded by

iyeswaryaa26
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd

NANOPORE

SEQUENCING
DNA SEQUENCING
● DNA sequencing is the process of
determining the precise order of nucleotides
in a DNA molecule.
● It unveils the specific order of adenine (A),
cytosine (C), guanine (G), and thymine (T)
along the DNA strand.
● This sequence carries the genetic
information vital for the growth,
functioning, and reproduction of organisms.
● To decode the genetic blueprint, aiding in
understanding traits, diseases, and
evolutionary connections.
NANOPORE
Nanopore sequencing utilizes tiny nanopores, which are minuscule channels at the nanometer scale, to sequence
DNA molecules.

❏ Biological Nanopores- Transmembrane protein channels


(alpha hemolysin)
❏ Solid state Nanopores- Silicon Nitride or holed Silica plate
filled with Fe
The magnitude of current depends on base composition (and size,shape of pore)
Place the nanopore (artificial) in a conducting solution
↓
Apply voltage
↓
Add nucleotides
↓
Drive the nucleotides through the nanopore (electrophoresis)
↓
Characteristic pattern of change in the pattern of current

Nucleotide: A T G C
Magnitude: ←↘ ↗ →
Use electrophoresis or Motor proteins to pull the nucleic acid through the nanopore

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