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Fish and Invertebrate Biology Lab Guide

The document contains a series of questions and tasks related to biology, including drawing and labeling a fish, estimating the number of scales, identifying invertebrates using a dichotomous key, and conducting an experiment with potato tubers in salt solutions. It also includes questions about enzyme activity and the effects of pH on trypsin. Each section is structured to assess knowledge and practical skills in biological concepts.

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0% found this document useful (0 votes)
32 views5 pages

Fish and Invertebrate Biology Lab Guide

The document contains a series of questions and tasks related to biology, including drawing and labeling a fish, estimating the number of scales, identifying invertebrates using a dichotomous key, and conducting an experiment with potato tubers in salt solutions. It also includes questions about enzyme activity and the effects of pH on trypsin. Each section is structured to assess knowledge and practical skills in biological concepts.

Uploaded by

zigzagmovment
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

1.

You are provided with a small fish


a. i. Draw the fish and label fins which control yawing.

3.marks
ii. Calculate magnification of your drawing

3. marks
b. Describe how you can estimate the number of scales the fish has.
_________________________________________________________________________________
_________________________________________________________________________________
_________________________________________________________________________________
_________________________________________________________________________________
_________________________________________________________________________________
________________________________________________________________________3 Marks
c. Some baby foods contain specimen A in its milled form as one of the ingredients. Of what nutritional
values is specimen A in baby foods.

(i)__________________________________________________________________________________
(ii) __________________________________________________________________________ 2 marks

Page 1
2. Figure 1 is a diagram showing some invertebrate animals. Use them to answer the questions that follow.

Figure 1

Key: 1. Wings present …………………………………..……….. see 2


Wings absent ………………………………..………….. see 4
2. Wings shorter than abdomen ………………..…..... see 3
Wings longer than abdomen …………..……………. Musca
3. Abdomen long and narrow …………..……………. Aedes
Abdomen short and broad …………..……………. Periplaneta
4. With three pairs of legs ………..………………. see 5
With four pairs of legs ……..………………… Ornithodorus
5. One pair of legs shorter than the other pairs ….. Pulex
All pairs of legs have similar length ………. Pediculus

a. Use the key to identify the name of each invertebrate in the diagram above.
A ______________________________________________________________________________
B ______________________________________________________________________________
C ______________________________________________________________________________
D ______________________________________________________________________________
E ______________________________________________________________________________
F _______________________________________________________________________ (6 marks)

Page 2
b. Using the names in the dichotomous key, mention any one organism which sucks human blood?
________________________________________________________________________________(1 mark)

c(i) Explain how organism ‘A’ is adapted to living on its host.


___________________________________________________________________________________
___________________________________________________________________________________(2 mark)

3. You are provided with the following materials;


 Irish potato tuber

 Salt solutions of the following concentrations 0%, 20% and 40% but not in that order. The solutions
are labelled P,Q and R.

 a knife or scapel

 a ruler

Procedure
_ Cut six equal-sized strips of irish potato each measuring 2.5cm long, 1cm wide and
0.5cm thick.
_ Put two strips into each of the containers (labelled P, Q and R) at the same time.
_ Leave to stand for 15 minutes.

a. After 15 minutes, measure the lengths of the two strips in each container and find the average length of the two
strips in each container.

Solutions Average length of the strips

Page 3
3 marks
(i) From the results above, which solution has the highest solute concentration?
__________________________________________________________________________________________
(1 mark)
(ii) Give a reason for your answer.
___________________________________________________________________________________________
(1 mark)
b (i) Salt as a solute has an osmotic effect. What does this mean?
___________________________________________________________________________________
___________________________________________________________________________________(1 mark)

(ii) Why are different salt concentrations being used in this experiment?
__________________________________________________________________________________________
(1 mark)
c. What evidence is there that the potatoes placed in salt solutions have lost water. Give
two points.
(i) ___________________________________________________________________________________
(ii) __________________________________________________________________________ (2 marks)

d(i) State any one possible source of error in this experiment.


_________________________________________________________________________________ (1 marks)
(ii) Explain one way in which the experiment has been designed to reduce errors.
_____________________________________________________________________________
___________________________________________________________________________ (2 marks)

Page 4
4. Trypsin is an enzyme which will digest the protein so that the coating on the photographic film is
removed and the film becomes clear. The table below shows the results obtained by two students who
investigated the effect of pH on the activity of this enzyme. They made up the solutions, set up the
experiment and timed how long the enzyme took to digest the protein and clear the film.

time for the protein to be digested / mins


pH
student 1 student 2

2 12.0 14.0

4 8.0 9.0

6 2.0 3.0

8 0.5 1.0

10 8.0 9.0

(a) Plot the results obtained by student 2 in the form of a suitable graph. 4.marks

(b) Describe the effect of pH on the activity of the enzyme.


___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
____________________________________________________________________3.marks

(c) Suggest a reason for the difference in the results for the two students.
______________________________________________________________________________ 1. mark
END OF QUESTION PAPER

Page 5

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