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Fibrinolysis Laboratory Test

The document outlines various laboratory tests for assessing fibrinolysis, including white blood clot lysis time, euglobulin clot lysis time, and D-dimer tests, each with specific principles and normal values. It differentiates between primary and secondary fibrinolysis and describes methods for detecting fibrin degradation products. The tests are crucial for diagnosing conditions related to abnormal clotting and fibrinolytic activity.

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72 views3 pages

Fibrinolysis Laboratory Test

The document outlines various laboratory tests for assessing fibrinolysis, including white blood clot lysis time, euglobulin clot lysis time, and D-dimer tests, each with specific principles and normal values. It differentiates between primary and secondary fibrinolysis and describes methods for detecting fibrin degradation products. The tests are crucial for diagnosing conditions related to abnormal clotting and fibrinolytic activity.

Uploaded by

jjkcpvjrbw
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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FIBRINOLYSIS LABORATORY TEST

Laboratory Test Principle Normal Value Pathologic Fibrinolysis


White blood clot lysis time →A clot is dissolved as a result of → 24 hours (gaanon katagal mag
plasmin activity. lalyse yung clot)
→Normally, this does not occur
in less than 72 hours because of
the presence of plasma inhibitors
which inactive plasmin as it forms
Euglobulin clot lysis time → euglobulin fraction of the → complete lysis < 2 hours Test for: Primary fibrinolysis
(screening test for fibrinolysis plasma (protein) contains
fibrinogen, plasminogen and all
of plasminogen activators but
only traces of antiplasmins.
→ The lysis of a fibrin clot formed
by the addition of thrombin is a
measure of the fibrinolytic
activity

Others:
→Screening test for fibrinogen
→ protein that is precipitated
when your plasma is added with
“diluted acetic acid”
→ dissolved in buffer with added
of thrombin
Diluted Blood clot lysis time → Plasmin inhibitors lose activity → Blood clot should NOT LYSE in
in dilution (whole blood) less than 2-10 hours.

Method:
→ Whole blood is diluted with a
buffer solution and clotted by the
addition of thrombin. Then the
clot is observed for lysis.
Diluted Plasma clot lysis → Serial dilutions of patient s → Lysis within 12 hours = means
plasma and normal plasma are increased fibrinolysis activity
prepared.
→ Thrombin is added to each
tube and is then observed later
on for presence of clot and
eventually for lysis
Quantitative assay of fibrin-
fibrinogen degradation products
Protamine sulfate turbidity → When a dilute solution of Positive result: Formation of Test: Secondary fibrinolysis
protamine sulfate is added to fibrin clot or fibrin strand
citrate plasma, and incubated at
37 c, a precipitate in the
presence of fibrin monomers or
early degradation products is
formed to produce turbidity (gel-
like clots)
Latex Bead agglutination test → a rapid, semi-quantitative
method to measure fibrin
degradation products.
D-dimer test → This test is superior in → This test is positive in early
sensitivity and specificity as Disseminated: Intravascular
compared with conventional FDP coagulation (DIC) and specific for
assay. cross-linked D-dimer fragment of
fibrin.
Prothrombin Fragment 1.2 Test → a new assay that is a sensitive
(F-12) biological marker of thrombin
generation and X activity
generation of F-12 precedes
thrombus because formation
Tanned Red cell → The FDP present in the Positive result: Absence of heme
Hemaggutination inhibition patients serum neutralizes agglutination
immunoassay antifibrinogen antiserum,
thereby preventing the
antiserum from agglutination
fibrinogen-coated erythrocytes
Ethanol Gelation test → detect presence of fibrin → Gel like CLT or PPT → Test for: secondary fibrinolysis
monomer in the plasma (Precipitate)

→ PLASMA-NAOHT ETHANOL

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