GOVT.
POLYTECHNIC NAYAGARH,
ODAGAON
Department Of Civil Engineering.
2023 – 2024
A SEMINAR PRESENTATION ON
Bacterial concrete
Submitted by
NAME:-SOMANATH NAhAK Under the guidance of
Regd no:- L22130001009 Sushree Sasmita sahoo
SEMESTER:- 4TH Dept. of civil engineering
BRANCH:- CIVIL
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INTRODUCTION
Cracks In concrete
Concretes are very susceptible to cracking which allows chemicals and
water to enter .
Cracking in the surface layer of concrete mainly reduces its durability.
When micro cracks growth reaches the reinforcement corrosion occurs
in the reinforcement.
Synthetic polymers such as epoxy treatments are used for repair of
concrete and they harm to the environment.
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BACTERIAL CONCRETE
Use of these Bio mineralogy concepts in concrete defined a new material
called “Bacterial Concrete”
Bacillus subtilis mixed with concrete in dormant stage and they remain
hibernated for 200 years.
Gets activated when exposed to water or moisture.
Precipitating calcite crystals through metabolism cycle the soluble
nutrients are converted to insolubleCaCO3.
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CHARACTERISTICS BACILLUS SUBTILIS
Characteristics Bacillus Subtilis
Shape, size, gram stain Long rods, 0.6-0.8 μm in width and 2.0 to 3.0 μm in length, gram
positive
Fermentation:
Lactose No acid, no gas
Dextrose No acid, no gas
Sucrose Acid and gas
H2S production Absent
Indole production Present
Colony morphology Irregular, dry, white, opaque colonies
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BACILLUS SUBTILUS SEM IMAGE
Bacillus subtilus Bacillus subtilus on agar platem
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HOW DOES BACTERIA REMEDIATE CRACKS?
Concrete is mixed with bacteria (bacillus subtilus) at dormant stage.
To survive in this high alkaline environment for long durations form spores (highly resistant
for 200 years ) withstanding during concrete mixing.
A calcium lactate nutrient supplied along with bacteria in suspension helps in producing
calcite crystals.
The spores of the bacteria germinate on contact with the water and nutrients while cracks
and get activated
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CONT.
As the bacteria feeds oxygen is consumed and the soluble calcium lactate is
converted to insoluble limestone.
The limestone solidifies on the cracked surface, thereby sealing it up.
Tests all show that bacteria embedded concrete has lower water and chloride
permeability by 88%
Reaction:
Ca 2+ + Cell --------> Cell- Ca 2+
Cell- Ca 2+ +CO3-----> Cell-CaCO 3
The crystals expand until the entire gap is filled.
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WHAT’S HAPPENING INSIDE BIO CONCRETE(Cont.)
(1) (2) (3)
The cracks are formed on the The water is deliberately forced into The activated precursor intem induces
surface of concrete due to many the crack and the precursor is the bacteria to react with that precursor
reasons like shrinkage, activated and form a base of calcium carbonate
Inadequate water for hydration called as the lime stone.
…ect
Ca(C3H5O2)2 + 7O2 → CaCO3 + 5CO2 + 5H2O
CO2 + Ca(OH)2 → CaCO3 + H2O
(carbonation)
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RESULTS BY RESEARCH PAPERS
Strength Study
Conventional Concrete Bio Concrete
@ 28 days age
M20 M30 M20 M30
Compressive Strength 28.18 37.14 32.74 41.06
Split Tensile Strength 3.26 4.51 3.93 5.13
Flexural Strength 4.68 5.11 6.11 6.73
Results in (Mpa) for Concrete and bacteria cell concentration 10^5/ml mixing water
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OPTIMUM CONCENTRATION OF CELL
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SEM IMAGES FOR CELL CONCENTRATION
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ADVANTAGES
Perpetual and expected cracking that occurs in every concrete structure due to its brittle
nature can be controlled.
Repairs without a human work crew.
Self-healing concrete also prevents the exposure of the internal reinforcements.
It has made to extend the life span of a concrete structure of any size, shape.
Material consumption reduced in form of cement.
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EVALUATION
agent is added, Optimization of healing agent is necessary so that it
wont affect the characteristics of concreteThe Concrete properties
should not be negatively affected by addition of a healing agent to the
concrete mixture.
a large amount of bacteria and certain mineral precursor compounds
like calcium lactate do substantially influence concrete compressive and
tensile strength
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CONCLUSION
Microbial mineral precipitation resulting improve the overall behaviour of concrete.
The greatest improvement in compressive strength occurs at cell concentrations of 10^5
cells/ml mixing water.
The study result shows that 25% increase in 28 day compressive strength.
Less permeability and corrosion resistance.
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REFERENCES
Ramachandran S.K., Ramakrishnan V. and Bang S.S., Remediation of concrete using
microorganisms, ACI Materials Journal 98(1), 3-9 (2001)
Stocks-Fischer S., Galinat J.K., and Bang S.S., Microbiological precipitation of
CaCO3. Soil Biology and Biochemistry, 31(11), 1563-1571 (1999)
Tai C.Y. and Chen F.B., Polymorphism of CaCO3 precipitated in a constant-
composition environment. AIChE Journal, 44(8), 1790-1798 (1998)
www.onlinelibrary.wiley.com/journal/10.1002/(ISSN)1547-5905
https://youtube.com/shorts/2eScG2xO9bE?feature=share
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REFERENCES
S. SUNIL PRATAP REDDY International Journal of Earth Sciences and
Engineering ISSN 0974-5904, Vol. 03, No. 01, February 2010, pp. 116-124.
V.Ramakrishnan, K.P.Ramesh and S.S.Bang, “Bacterial Concrete”Proceedings
of SPIE Vol.4234 pp.168-176, Smart Materials.
Thank You
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