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Platelet Count

This document provides information about platelet counting methods. It describes the specific learning objectives which are to describe platelet counting methods, explain the principle and procedure of manual counting using a hemocytometer, and know normal values and clinical significance. It then explains the manual method using an improved Neubauer chamber, platelet diluting fluids, and the procedure for manual counting. Normal ranges and some clinical implications of thrombocytopenia and thrombocytosis are also summarized.

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Thabshira
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© © All Rights Reserved
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Available Formats
Download as PPTX, PDF, TXT or read online on Scribd

Topics covered

  • Laboratory Equipment,
  • Research Methods,
  • Assessment Techniques,
  • Dacie’s Fluid,
  • Medical Education,
  • Quality Control,
  • Polycythemia Vera,
  • Hemocytometer,
  • Brecher-Cronkite Fluid,
  • Chronic Myeloid Leukemia
2K views18 pages

Platelet Count

This document provides information about platelet counting methods. It describes the specific learning objectives which are to describe platelet counting methods, explain the principle and procedure of manual counting using a hemocytometer, and know normal values and clinical significance. It then explains the manual method using an improved Neubauer chamber, platelet diluting fluids, and the procedure for manual counting. Normal ranges and some clinical implications of thrombocytopenia and thrombocytosis are also summarized.

Uploaded by

Thabshira
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd

Topics covered

  • Laboratory Equipment,
  • Research Methods,
  • Assessment Techniques,
  • Dacie’s Fluid,
  • Medical Education,
  • Quality Control,
  • Polycythemia Vera,
  • Hemocytometer,
  • Brecher-Cronkite Fluid,
  • Chronic Myeloid Leukemia
  • Introduction: Introduces the topic of platelet count including contributors and institutional affiliation.
  • Lesson Plan: Presents the lesson plan, aiming to provide a structured approach to learning about platelet count.
  • Learning Objectives: Lists the specific learning objectives for the training session on platelet count methods.
  • Measurement Methods: Describes the methods employed for measuring platelet count in blood samples.
  • Procedure: Details the step-by-step procedure for conducting a platelet count manually.
  • Calculating Counts: Explains how to calculate platelet counts from chamber observations.
  • Normal Range and Significance: Discusses the normal range of platelet counts and their clinical significance.
  • Summary: Summarizes key points about platelet counting methods and their importance in hematologic investigation.
  • Frequently Asked Questions (FAQ): Provides common questions and answers regarding platelet count techniques and knowledge.
  • References: Lists academic references supporting the content of the platelet counting tutorial.
  • Thank You: Final slide showing gratitude for the reader's attention.

PLATELET COUNT

Prepared by
Reviewed by:
Mr. Mohideen Sharfan S A
PPT Review committee
Assistant Professor
YSAHS
Medical Laboratory Technology

YSAHS
LESSON PLAN
Specific learning objective:
At the end of the session student should be able

to:
 Describe different methods used for Platelet count.

 Explain the principle and procedure of manual method,

platelet count by using hemocytometer.


 To know the normal values and the abnormal clinical

significance.
Platelet count in the blood can be measured either

by manual method using an improved neubauer


chamber or by automated analyzer.
Manual Method
Improved neubauer counting chamber.

RBC pipette.
Principle
Anticoagulated blood is diluted with a diluent that

lyses the red cells(1% Ammonium Oxalate) leaving


visible platelets.
Platelet Diluting Fluids
Dacie’s Fluid

Rees-Ecker Fluid

Brecher-Cronkite Fluid: consist of 1% ammonium

oxalate solution which is filtered and stored at 4c.


Composition of Dacie’s fluid:

constituents quantity
Sodium citrate 5.0g
dihydrate
formalin 1ml
Distilled water 100 ml
Procedure
Gently mix the blood in the EDTA vial so that the
cells mix well with plasma.
Draw the blood up to 0.5 mark of RBC pipette.

Dip the tip of the pipette in the 1% Ammonium


Oxalate and draw the fluid upto mark 101.
Holding the pipette horizontally in its long axis,
rotate it slowly to ensure through mixing of blood
and diluent.
Place the cover slip on the ruled area of the chamber

Discard the first 2 to 3 drops of RBC fluid from the

pipette.
Charge the chamber by placing the tip of the pipette

just under the coverslip and allow the fluid to flow by


capillary action till the counting chamber is just
filled.
Keep the charged chamber in a petridish plate with

wet filter paper at the bottom for 5 minutes.


Platelets are counted entire central 1×1mm square.

Platelets appear is highly retractile small

structures.
Platelet count = total counted platelets in the

central square × 200 per cumm.


Normal Range
1,50,000 to 4, 50,000 platelets per cumm.

Decreased platelet count is known as

thrombocytopenia.

Increased platelet count known as

thrombocytosis.
Clinical significance
Thrombocytopenia:
Acute immune thrombocytopenic purpura.

Chronic immune thrombocytopenic purpura.

SLE.

DIC.
Thrombocytosis:
Essential Thrombocytosis.

Polycythemia Vera.

Chronic myeloid Leukemia.


Summary
 Platelets are the formed elements of blood.

 Estimation of platelet counts is an essential hematologic

investigation which may be performed by manual methods


or by automated analyzers.
 The improved Neubauer chamber, RBC pipette and

diluting fluids are used for manual counts of any cell.


FAQ
 Write the principle and procedure of manual method of

Platelet count. (4)

 Causes of increased platelet count. (2)

 Composition of platelet diluting fluid. (2)


References
 Nayak R, Rai S. Essentials in Hematology and clinical

pathology.2nded.India:Jaypee the health science


publisher;2017.
THANK YOU

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