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HPLC and GC by S Narwadiya

HPLC and GC by S Narwadiya



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Published by snarwadiya
It shows in short the methods of HPLC and GC
It shows in short the methods of HPLC and GC

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Published by: snarwadiya on May 18, 2009
Copyright:Attribution Non-commercial


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GC is useful for compounds which arenaturally volatile or can be converted intoa volatile form. 
GC has been a widely used method due toits high resolution, low detection limits,accuracy and high resolution limits, andshort analytical time.
Retention of a compound is determined byits vapour pressure and volatility which, inturn, depends on its interaction withstationary phase.
Two types of stationary phases arecommonly used in GC are solid absorbent(Solid Gas Chromatography [GSD]), andliquid coated on solid support (Gas-LiquidChromatography [GLC]).
In GSC same material (usually silica,alumina, or activated carbon) act as boththe stationary phase and support phase.
GLC uses liquid phases such as polymers,hydrocarbons, fluorocarbons, liquidcrystals, and molten organic salts to coatthe solid support materials.
Calcin diatomaceous earth graded intoappropriate size ranges used as astationary phase because it is stableinorganic substance.
Components of a typical GC systemconsists
components: a gascylinder as a mobile phase source, asample injector, a column, a detector,and a computer for data acquisition.
The mobile phase used in GC usuallyinert gas such as nitrogen, helium,hydrogen, argon, carbon dioxide,nitrous oxide and ammonia. 

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