Gas Chromatography

BY : ASHISH THAPA MAGAR

 Introduction

Gas chromatography is also known as vapour-

phase chromatography .
It is used to separate compounds that are volatile
or can be made volatile
It can be gas-solid chromatography (GSC) with a
solid stationary phase or gas-liquid
chromatography (GLC)with a non-volatile liquid
stationary phase .
 GLC is commonly used in clinical laboratories.
 Gas liquid chromatography

In GLC the stationary phase is liquid and the

mobile phase is gas.
The mobile phase (gas ) can be helium, nitrogen or
argon.
The stationary phase(liquid) is supported by a
column of inert materials such as silica in a long
narrow column.
The instrument can be operated in constant
temperature or programmed to run in different
temperature (sample with different votalities).
GLC
 Cont.

The sample to be injected must be gas or made

volatile by high temperature.
Sample vapour is swept through the column
partially as gas and partially dissolved in the liquid
phase.
Volatile compounds the are present mainly is gas
phase will have low boiling point and move quickly
through the column.
Compounds with higher boiling point will move
slowly through the column.
 Cont.

The elute that passes through the detector produces

an electric signal in proportion to the conc. of the
volatile components.
The chromatogram is used both to identify the
compounds by their retention time and conc.
This is more suitable for compounds (e.g. lipids )
which resist degradation at high temperature.
 Reference :

Textbook of Biochemistry for Medical Students , DM

Vasdevan
Biochemistry , Dr. U. Satyanarayana , Dr. U.
Chakrapani
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