Bioanalytical Solutions

Bioanalytical Solutions
Bioanalytical Systems
UltiMate™ 3000
nert BioLC—Nano to Micro
ICS-3000™
Analytical to Semiprep HPLC
Metal-Free BioIC/BioLC
APS-2000 and APS-3000 Analytical
Maximized Autopurification
Semiprep to Preparative DX-800™ PAT
Process IC and LC
Analytical
Performance, Reliability,
Bioanalytical and Ease-of-
Systems
Use
UltiMate 3000
™

ICS-3000™
Process IC and LC
Analytical
UltiMate™ 3000 LC
Nano/Cap/Micro,
inert
Binary Micro, inert
Quaternary Micro,
inert
Binary
Analytical
Quaternary
UltiMate™ 3000 LC
New for 2007!
Well Plate Sampler

with Fraction
Collection
Pharma/Biotech Columns
ProSwift™ ProPac®
Monolith Technology Protein Separations
CarboPac® Acclaim®
Mono to Tailored Selectivity (Si)
Oligosaccharides
AminoPac® DNAPac®
AAA-Direct™ High Efficiency for Oligos
Acclaim ®
CarboPac Fast LC
Acclaim
® ®
!
Mono to 0 7
Tailored Selectivity (Si)
Oligosaccharides 2 0
AminoPac® DNAPac fo ®
r
AAA-Direct™
ew
High Efficiency for Oligos
N
New Acclaim® Fast LC Columns
4.6 x 150 mm 12 minutes
3 x 75 mm 2.7 minutes
3 x 33 mm 0.8 minutes
15x Speed with Equivalent Resolution

UltiMate™ 3000 LC
Nano/Capillary/Micro
Binary Micro
Quaternary Micro
All with x2 Dual Technology
Designed for
Better
Mass
Spectrometry
Oligosaccharides
AminoPac® DNAPac®
Acclaim
Monolith Technology Protein
® Separations
CarboPacMixedAcclaim
Mode
WAX-1
® ®
7 !
Mono to 0
Tailored Selectivity
0
(Si)
Oligosaccharides
2
AminoPac ®
DNAPacfor ®
AAA-Direct ™
e w
High Efficiency for Oligos
N
Acclaim® Mixed-Mode WAX-1
ultaneous Separation of Acidic, Basic, and Neutral Molecu

Adjustable Selectivity: Ionic
Strength
pH
Organic
Modifier
Move Only the Anion When Adjusting the Ionic Strength

Strength
pH
Organic
Modifier
Move Only the Anion When Adjusting the pH

Strength
pH
Organic
Modifier
Total Control of Selectivity

Selectivity Orthogonal to Reversed-Phase

Bioanalytical Solutions
UltiMate™ 3000
nert LC—Nano to Micro
ICS-3000™
APS-2000 and APS-3000 Microbore to Analytical
Process IC and LC
Analytical
PEEK inert ICSystems
Bioanalytical and LC
UltiMate™ 3000
nert LC—Nano to Micro
ICS-3000™
APS-2000 and APS-3000 Microbore to Analytical
Process IC and LC
Analytical
ICS-3000 IC and LC System
 Microbore to
analytical flow
 Metal-free PEEK
flow path
 Single or dual pump
 Multiple detector
options
ICS-3000 3D Amperometry
with the
PDA-type unique
information selectivity
of electro-
chemical
detection
 The derivatization-
free solutions for
– Amino acids
– Carbohydrates
– Sugar alcohols
– Amino sugars
 Perfectly inert
– To handle
corrosive
mobile phases
 Perfectly
inert
– To protect
bioactivity
Oligosaccharides
AminoPac® DNAPac®
ProSwift™
SAX and
CarboPac WCX
Acclaim
® ®
!
Mono to
0 7
Tailored Selectivity (Si)
Oligosaccharides
2 0
AminoPac® DNAPac or
f
®
AAA-Direct™ High w
Efficiency for Oligos
N e
ProSwift™ Monolith Morphology
Through Pores Non-porous Globule
High Permeability Low Backpressure High Flow Rate

Flow-Through Pores Fast Mass Transfer High Resolution
ProSwift™ SAX Column: Protein Mix
900 1
1. Myoglobin 100.0 Column: SAX (4.6 x 50
2. Conalbumi mm)
n
3. Ovalbumin
Flow Rate: 1.5 mL/min
4. Trypsin Eluents: A. 10 mM Tris (pH
inhibitor
3 % 50.0 7.6)
B B. 10 mM Tris +
1M
2 4
5 minutes NaCl (pH 7.6)
–100
0 Minutes 9
Monolith Technology for Speed and

Efficiency
ProSwift™ WCX Column: MAb
600
1
Variants
Lys Variants
100.0 Column: WCX (4.6 x 50
mm)
2
Flow Rate: 1 mL/min
3 Eluents: A. 10 mM
mAU
Na2HPO4
(pH 7.6)
Acidic Variants Basic Variants
B. 1M NaCI in
eluent A
%B 10.0
MAb 5 mg/mL
5.2
–100
0 Minutes 14.1
Monolith Technology for High Capacity Separations

UltiMate™ 3000
ICS-3000™
Process IC and LC
Analytical
Bioanalytical
Performance, Systems
Reliability, and Ease-of-
Use
UltiMate 3000
™

ICS-3000™
Process IC and LC
Analytical
x2 Dual LC Systems
x2 Dual-
Nano/Cap/Micro
x2 Dual-Binary Micro
x2 Dual-Ternary
Micro
x2 Dual-Binary
Analytical
x2 Dual-Ternary
UltiMate™ 3000 x2 Dual HPLC
 More productivity
– Tandem column
mode
– Paralleluse-time
 Increased column
mode
– Application
switching
 On-line sample prep
– Heartcut mode
– Backflush mode
 Higher selectivity
– Multidimensional
LC
x2 Dual-Channel Parallel Mode
Increase Throughput by 100%
Analysis Channel 1
Analysis Channel 2
0 Minutes 20
Without Changing Validated Methods
Use the Full Power of Reliable 2-D
LC TCC 100 MSV 2P
Pump #1 IV Fe3+10P
IMAC Step 1 Step 3
HCO2H,
pH 3H, pH
NH4HCO
IMAC Fraction IMAC Fraction
Collection
9
2
Loop 400 Non-phospho- Phosphopeptides

Pump #2 µLw peptides
5% MeCN, 0.1%
TFA
70% MeCN, 0.1%
10- Detector
TFA Port UV
UltiMate Valve 214 nm
3000
w
x2 Dual-
Acclaim C18
Ternary 0 1 2 0 1 2
Minutes Minutes
Step 2
RPC of Non-phosphopeptide Fraction Step 4
RPC of Phosphopeptide Fraction
5 10 15 5 10 15
Minutes Minutes
Simplify and Improve Sample
Preparation
Direct Injection of Human Plasma
Pump A Pump B
Autosampler Dual Gradient

Pump Itraconazole
1-OH-Itraconazole
50
40
Analytical 30
RAM Column
mA
20 Voriconazole Wash
U
Column RAM (RP/SEC) Ketoconazole SPE Recondition
10Fractionation SPE Column
Column
0
Transfer from SPE Column
–10 Separation on Analytical Column mi
n
RAM: Restricted Access Material 0 2 4 6 8 10 13
UV-Vis Detector Minutes
Chromeleon® Control of Every
Component

Bioanalytical Solutions - Dionex

Uploaded by

Document Information

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Bioanalytical Solutions - Dionex

Uploaded by

Copyright:

Available Formats

nert BioLC—Nano to Micro

New for 2007!

Well Plate Sampler

4.6 x 150 mm 12 minutes

15x Speed with Equivalent Resolution

ultaneous Separation of Acidic, Basic, and Neutral Molecu

Move Only the Anion When Adjusting the Ionic Strength

Move Only the Anion When Adjusting the pH

Total Control of Selectivity

Selectivity Orthogonal to Reversed-Phase

High Permeability Low Backpressure High Flow Rate

Monolith Technology for Speed and

Monolith Technology for High Capacity Separations

nert BioLC—Nano to Micro

Loop 400 Non-phospho- Phosphopeptides

Autosampler Dual Gradient

You might also like