2012 ECatalog ROW - 00 Entire Catalog

B2011-12691
www.coulterow.com
1
2012 Cellular Analysis Catalog
1 1
Outside US
Antibodies to Human Antigens------------------- 61-244
Antibodies to non-Human Antigens ------------ 245-321
Immune Monitoring ------------------------------ 357-385
Cell Signaling ------------------------------------ 323-356
Support Reagents -------------------------------- 387-431
Information ------------------------------------------ 3-10
Indices ------------------------------------------- 433-473
Instrumentation ------------------------------------ 11-60
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Format and Lines -------------------------------- 4-8
Trademarks --------------------------------------- 10
Regulatory Status --------------------------------- 9
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Outside US Cellular Analysis Catalog
Flow Cytometry Reagents
Beckman Coulter provides a variety of cellular analysis reagents to meet the needs of todays flow cytometry
laboratories. We offer several complete systems that combine reagents, calibrators, controls, software and hardware.
Flow cytometry reagents are typically provided as directly conjugated products and are available as single color
configurations or as two, three, four or five color combinations.
These comprise hundreds of products allowing sample preparation flexibility as well as panel customization.
Format and Lines
Abbreviation Name Primary Appropriate Light Emission Suitable Band
Excitation Source Maximum Pass Filter
(nm) (nm) (nm)
Alexa Fluor* 488 Alexa Fluor* 488 495 Blue Diode/Argon Laser 520 525
Alexa Fluor* 647 Alexa Fluor* 647 650 Red Diode/HeNe Laser 668 675
Alexa Fluor* 700 Alexa Fluor* 700 696 Red Diode/HeNe Laser 719 720
APC Allophycocyanin 650 Red Diode/HeNe Laser 660 675
APC-Alexa Fluor* 700 APC-Alexa Fluor* 700 650 Red Diode/HeNe Laser 719 720
APC-Alexa Fluor* 750 APC-Alexa Fluor* 750 650 Red Diode/HeNe Laser 780 780
CY*5 Cyanin 5 580 Red Diode/HeNe Laser 670 675
ECD Phycoerythrin-Texas Red*-x 480 Blue Diode/Argon Laser 620 620
FITC Fluorescein Isothiocyanate 495 Blue Diode/Argon Laser 520 525
FLMA Fluorescein 5-Maleimide 495 Blue Diode/Argon Laser 520 525
Krome Orange Krome Orange 398 Violet/405 nm Diode Laser 528 550
Pacific Blue* Pacific Blue 405 Violet/405 nm Diode Laser 455 450
PC5 Phycoerythrin-Cyanin 5 480 Blue Diode/Argon Laser 670 675
PC5.5 Phycoerythrin-Cyanin 5.5 480 Blue Diode/Argon Laser 694 700
PC7 Phycoerythrin-Cyanin 7 480 Blue Diode/Argon Laser 767 770
PE Phycoerythrin 480 Blue Diode/Argon Laser 575 575
PI
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Propidium Iodide 540 Blue Diode/Argon Laser 620 620
RD1 Phycoerythrin 480 Blue Diode/Argon Laser 575 575
TRITC Tetramethyl Rhodamine Isothiocyanate 555 Hg Arc Lamp or Green Laser 580 575
TXRD Texas-Red-x 575 Hg Arc Lamp or Yellow Laser 620 620
7-AAD
1
7-Aminoactinomycin D 550 Blue Diode/Argon Laser 660 675
AP Alkaline Phosphatase
BGAL -galactosidase
UNLB Purified, unconjugated BIMA Biotin Maleimide
BIOT Biotin
HRP Horseradish Peroxidase
Purified Purified, unconjugated
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As DNA Complex
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Format and Lines
CYTO-STAT Monoclonal Antibodies
CYTO-STAT Monoclonal Antibodies are :
Ready-to-use monoclonal antibodies for flow cytometric analysis.
Optimized for use with the ImmunoPrep no wash lysis method.
Available in one, two, three and four-color configurations for direct immunofluorescent staining.
Specially formulated for use on the Q-Prep or TQ-Prep Workstations, the PrepPlus 2 Workstation or the
FP 1000 Cell Preparation System with the ImmunoPrep Reagent System.
Provided as 50 tests per vial at 10 L per test (0.5 mL/vial)
Source: Mouse ascitic fluid or conditioned culture media unless otherwise noted.
Purification: Ion-exchange, affinity chromatography or gel filtration.
Components and storage: supplied in liquid, ready-to-use form requiring no dilution. Store at 2-8C in the
dark without freezing. Expiration date and concentration are indicated on the vial label.
IMMUNOPREP Reagent System
No-wash lyse and fix reagent for whole blood lysis.
Maintains white blood cell integrity and natural scatter characteristics.
Maximizes separation between cells of interest versus red blood cell debris and platelets.
Twenty-four hour processed-sample stability when stored at 2-8C.
IOTest and IOTest3 Conjugated Antibodies
IOTest Conjugated Antibodies are :
Ready-to-use conjugated monoclonal or polyclonal antibodies for flow cytometric analysis.
Adapted for manual lysing procedures with a wash and for automated lysing procedures on the PrepPlus 2
Workstation and the FP 1000 Cell Preparation System.
Open to all instrument platforms.
Available as single or multi color configurations for direct immunofluorescent staining.
Single color reagents are titrated for easy mixing in user-defined multi-color combinations.
Provided as 100 or 50 tests per vial at 10 or 20 L per test.
Components and storage: supplied in phosphate-buffered saline, containing 0.1% sodium azide and 2
mg/mL of bovine serum albumin, unless otherwise specified. The expiration date is indicated on the vial label.
Store at 2-8C in the dark without freezing.
IOTest3 Conjugated Antibodies are
Preformulated and standardized product line composed of antibodies conjugated to FITC, PE and ECD.
Cover the most useful markers for peripheral blood or bone marrow characterization exploring intracellular as
well as surface antigens.
Optimized to work with the IOTest 3 Lysing Solution and IOTest 3 Fixative Solution.
IntraPrep Permeabilization Reagent is recommended for intracellular antigen exploration.
Provided as 25 tests per vial at 20 L per test.
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Format and Lines
OptiClone Monoclonal Antibodies
OptiClone Monoclonal Antibodies are :
Ready-to-use monoclonal antibodies for flow cytometric analysis.
Available in two and three color configurations for direct immunofluorescent staining.
Specially formulated for use in wash or no-wash procedures using OptiLyse B or C Lysing Reagents.
For individual monoclonal antibody specifications, see the product description.
Components and storage: supplied in phosphate-buffered saline, containing 0.1% sodium azide and
2 mg/mL of bovine serum albumin, unless otherwise specified. Expiration date and concentration are indicated
on the label. Store at 2-8C in the dark without freezing.
OptiLyse Lysing Reagent
OptiLyse C is specially formulated for Beckman Coulter flow cytometers.
OptiLyse B is specially formulated for BD Biosciences flow cytometers.
Provided as 200 or 250 tests per vial, at 500 L per test for OptiLyse C and 100 L per test for OptiLyse B.
Suitable for wash or no-wash applications.
COULTER CLONE Monoclonal Antibodies
COULTER CLONE Monoclonal Antibodies are :
Suitable for flow cytometric, microscopic or immunohistochemical procedures.
Available as purified and fluorochrome-conjugated antibodies for direct immunofluorescent staining.
Purified and most FITC antibodies are lyophilized and require reconstitution in 500 L of distilled water.
RD1 conjugated antibodies are liquid and ready-to-use.
Components and storage: supplied in phosphate-buffered saline solution, containing 0.1% sodium azide and
2 mg/mL of bovine serum albumin, unless otherwise specified. The freeze-dried material must be
reconstituted with distilled water without preservative as described in the package insert. Expiration date and
concentration are indicated on the vial label. Store at 2-8C in the dark without freezing.
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Format and Lines
Cell Lab Reagents
Cell Lab: Cell Lab reagents are a selection of specialized tools, which equip researchers with the resources necessary
to fulfill their cellular analysis needs. These reagents are provided in various forms and sizes and may be used for a
wide range of applications including cell biology and functional studies. The Non-Human Species Specific
Antibodies may be used for detection of surface and intra-cellular proteins as well as a number of secreted proteins
such as cytokines.
Cell Lab Select: Cell Lab Select reagents are a selection of Cell Lab antibodies conjugated to tandem dyes utilizing
Beckman Coulter patented tandem dye technology. Beckman Coulter tandem dye technology features;, optimized
donor acceptor binding for reduced donor dye bleed, a proprietary formulation to reduce non-specific binding of
tandem dyes to monocytes and enhanced light stability utilizing a patented vial coating. Cell Lab Select reagents are
available for mouse and human immunophenotyping studies.
Signaling Cytometry (SC): Use of one or more signal transduction markers in conjunction with cell surface markers
to dissect complex biological processes in heterogeneous cell populations. The Activation State Specific markers
may be used to identify cell signaling processes that take place within cellular sub populations.
Cell Lab SC: Cell Lab SC reagents are a selection of antibodies targeting intracellular targets such as signal
transduction markers. Labeling of these markers may be used to identify cell signaling processes taking place within
cellular sub populations. These reagents are validated for multicolor flow cytometric analysis. Cell Lab SC reagents
are supplied as ready use conjugates to Alexa Fluor 488, Alexa Fluor 647.
Other Reagents
A large range of purified and biotinylated antibodies are provided in various formats adapted for multiple
applications: cell biology, immunohistochemistry, functional studies, pharmacology, Western blot,
immunoprecipitation, and immunostaining. Most clones in this catalog are available in the purified format. The
lyophilized format (0.1 or 0.2 mg) permits quantitative experiments. The liquid format, sold as a number of tests, is
specifically adapted to immunostaining experiments. Many of these products are available for bulk purchase.
Source: Polyclonal antibodies from rat, rabbit, goat, and hamster. Monoclonal antibodies from murine ascitic
fluid or conditioned culture media.
Components and storage: In the freeze-dried format, most of the antibodies are supplied in phosphate-
buffered saline solution, containing 1 mg/mL bovine serum albumin, without preservative. The freeze-dried
material must be reconstituted with distilled water as described in the package insert. Aliquots may be stored
at -20C. Avoid repeated freezing and thawing. The addition of sodium azide (0.1%) is recommended for
storage up to one month at 2-8C. In the liquid format, antibodies are supplied in phosphate-buffered saline
solution, containing 0.1% sodium azide and 2 mg/mL of bovine serum albumin, unless otherwise specified.
The expiration date is indicated on the vial label. Storage is at 2-8C in the dark without freezing. Some other
formats are available, please refer to the product list for more information.
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Immunohistochemistry Reagents
Beckman Coulter provides several primary antibody formats to address most working situations.
IOPath , IOPath+ Reagents
IOPath and IOPath+ are optimized and calibrated for immunostaining of tissue sections using the Universal
Streptavidin-Biotin system (see Support Reagents/Detection Kits).
IOPath antibodies are ready-to-use in a 6 mL liquid format
IOPath+ antibodies are concentrated in a 1 mL liquid format. They are calibrated to be used at a final dilution
of 1/50.
Source: Mouse ascitic fluid or conditioned culture media, or rabbit antiserum.
Purification: Ion-exchange chromatography, affinity chromatography or gel filtration.
Components and storage: Prediluted antibodies, IOPath are supplied in 50 mM Tris-HCl, 150 mM NaCl,
pH 7.2, containing 1 mg/mL of bovine serum albumin and 0.1% sodium azide. The buffer contains a green
dye, except for part #IM1344 (Estrogen receptor) which is formulated with a pink dye. Storage is at 2-8C,
without freezing. The expiration date is printed on the vial label.
Concentrated antibodies, IOPath+ are supplied in phosphate-buffered saline containing 2 mg/mL of bovine
serum albumin and 0.1% sodium azide. Storage is at 2-8C, without freezing. The expiration date is printed
on the vial label. Dilution should be performed in a protein-containing buffer (2 mg/mL bovine serum
albumin or equivalent).
Other Reagents
Concentrated primary antibodies are available in liquid or freeze-dried formats. They are not restricted to
immunohistochemistry. These products are provided with a fixed protein concentration for the widest range of
experimental applications.
Source: Mouse ascitic fluid or conditioned culture media, or rabbit antiserum.
Purification: Ion-exchange chromatography, affinity chromatography or gel filtration.
Components and storage: Concentrated freeze-dried antibodies are supplied in phosphate-buffered saline
solution, containing 1 mg/mL of bovine serum albumin, with no preservative, unless otherwise specified.
They must be reconstituted with distilled water as described in the package insert.
Concentrated liquid antibodies are supplied in phosphate-buffered saline solution containing 2 mg/mL of
bovine serum albumin and 0.1% sodium azide unless otherwise indicated in the package insert.
Concentrated freeze-dried and liquid antibodies: 2-8C or in aliquots at -20C. Avoid repeated freezing and
thawing. The addition of 0.1% sodium azide is recommended for storage up to one month at 4C.
Dilution of concentrated reagent should be performed in protein-containing buffer (2 mg/mL bovine serum
albumin or equivalent).
Format and Lines
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Regulatory Status
Analyte Specific Reagents (ASR)
An ASR is the active ingredient of an in-house (home-brew) test. It is the
responsibility of the laboratory using the ASR to develop the test and to take
responsibility for establishing and maintaining performance. These reagents are
labeled Analyte Specific Reagents. Analytical and performance characteristics are
not established.
CE-marked products (CE)
CE is an abbreviation of the French phrase Conformit Europenne. CE
marking on a product indicates conformity to the legal requirements of the
European Union (EU) Directive 98/79/EC of the European Parliament for in vitro
diagnostic medical devices with respect to safety, health, environment, and
consumer protection. CE-marked products are labeled for in vitro diagnostic use
and can be traded freely throughout the EU.
In Vitro Diagnostic Products (IVD)
Reagents, instruments and systems intended for use in the diagnosis of disease or
other conditions, including a determination of the state of health, in order to cure,
mitigate, treat or prevent diseases. Such products are intended for use in the
collection, preparation and examination of specimens taken from the human body.
These products can be used for clinical diagnosis. These products are labeled For
In Vitro Diagnostic Use.
In Vitro Diagnostic Products & CE-Marked Products (IVD & CE)
These reagents comply with the IVD and CE classifications above and are
distributed worldwide for in vitro diagnostic use.
Laboratory Use Only Reagents (LUO)
Generic reagents whose use is generally known by a trained person. Not intended
to identify a specific disease or condition. These reagents are labeled For
Laboratory Use Only.
Research Use Only Reagents (RUO)
A product in the laboratory testing phase of development that does not have a
recognized clinical utility and is not represented as an effective in vitro diagnostic
product. These products cannot be used for clinical diagnosis, prognosis or
therapeutic determinations. These reagents are labeled For Research Use Only.
Not for use in diagnostic procedures.
Medical Device (MD)
MD (93/42/EEC) - CE marked in conformity with the European Medical Devices
Directive 93/42/EEC
ASR
CE
IVD
IVD & CE
LUO
RUO
MD
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The following are trademarks of Beckman Coulter, Inc.:
Trademarks
AccuSort
Allegra
AllSpin
Avanti
ARIES
Astrios
BECKMAN COULTER
Biomek
Cell Lab Quanta
CellProbe
Cell-Seeker
Cell-Stat
Cell-Tracker
COULTER
COULTER CLENZ
COULTER CLONE
COULTER COUNTER
CyAn
CYTO-COMP
Cyto-Spheres
CYTO-STAT
CYTO-TROL
DNA PREP
DNA Prep
ECD
Elite
Elite ESP
EPICS
Flow-Check
Flow-Clean
Flow-Comp
Flow-Count
Flow-Set
Gallios
HarvestLine
ICD-PREP
IMMUNO-BRITE
IMMUNOPREP
IMMUNOTECH
IMMUNO-TROL
Index
INSIGHTOMETRY
IntraPrep
IOPath
IOTest
IsoClonic
IsoFlow
ISOTON
iTAg
iTopia
J-LITE
Kaluza
Krome Orange
Manual CD4 Count
MoFlo
MULTI-Q-PREP
Navios
OptiClone
OptiLyse
Optima
PrepPlus
Q-PREP
Retic-CAL
reticONE
Retic-STAT
Solastra
SortSense
Stem-Comp
Stem-Count
Stem-Kit
stemONE
Stem-Trol
System II
tetraCHROME
tetraONE
ThromboFix
TQ-Prep
triCHROME
VersaLyse
Vi-Cell
XL
XL-MCL
Z Series
Other Trademarks:
* Trademarks are the property of their respective owners
CY5 is a trademark of Amersham Biosciences. Gleevac is a registered trademark of Novartis AG. SpectralRed, ApoScreen,
and Fluoromount-G are trademarks of Southern Biotech. The PCR process is covered by patents owned by Roche Molecular
Systems and E. Hoffman-LaRoche, Ltd. PermaFluor and DynaChrome are trademarks of Thermo Electron Corporation.
CellQuant and RedQuant are trademarks of BioCytex. Instrument Manager is a trademark of Data Innovations, Inc.
HyperCyt is a registered trademark of IntelliCyt Corporation. iCys and iCyte are registered trademarks of CompyCyte
Corporation. Quantum is a trademark or registered trademark of CaridianBCT,Inc. in the United States and in several other
jurisdictions. Texas Red and Pacific Blue are trademarks of Molecular Probes, Inc. AlexaFluor: This product is provided
under an agreement between Molecular Probes, Inc. and Beckman Coulter and the manufacture, use, sale or import of this
product may be subject to one or more of US Patent Nos. 5,696,157; 6,130,101; 5, 830,912; pending applications and
corresponding foreign equivalents, owned by Invitrogen Corp. The purchase of this product conveys to the buyer the non-
transferable right to use the purchased amount of the product and components of the product in research conducted by the
buyer (whether the buyer is an academic or for-profit entity), including use as an Analyte Specific Reagent (ASR), but
excluding use in combination with microarrays or for High Content Screening. The buyer cannot sell or otherwise transfer
(a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise
use this product or its components or materials made using this product or its components for Commercial Purposes.
Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the
product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or
data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the
product or its components, whether or not such product or its components are resold for use in research. XP is a trademark of
Cell Signaling Technologies, Inc.
Streptamer technology is covered by pending patent applications exclusively licensed to IBA GMbH, Germany
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Instrument and Software -------------------------- 12-28
Cell Preparation Systems -------------------------- 29-33
Fluorospheres and Quality Controls --------------- 34-39
Lysing, Fixative and Permeabilizing Solutions ---- 40-44
Systems and Kits ----------------------------------- 45-57
Instrument Accessories -------------------------------- 58
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Instrument Support Reagents --------------------- 59-60
Instruments and Software
Designed to fit your needs, today and tomorrow, the Gallios flow cytometer provides efficient acquisition of superior quality data from up
to 10 colors with advanced optical design for enhanced sensitivity for multicolor assays. With customized forward scatter, to achieve
the most accurate size measurement, and optimized electronics for high resolution and fast, accurate processing, the Gallios was
designed for your research needs. Gallios is optimized for unparalleled signal to noise resolution in all parameters and its quick and easy
setup of automated walk-away processing improves your workflow while its remote diagnostics minimize downtime and allow you to
maximize lab productivity.
Gallios Flow Cytometer
Optics
Lasers/Power Output
- Blue Solid State Diode, 488 nm, 22 mW
- Red Solid State Diode, 638 nm, 25 mW
- Violet Solid State Diode, 405 nm, 40 mW
- 125 m spatially separated beam spots
Flow Cell
- 150 x 460 m quartz, gel coupled 1.2 NA lens
Fluorescence Delivery
- Highly efficient multi-mode fiber optics to maximize
light delivery
- Easy interchangeable 18-degree tilted optical filters
Detectors
- Forward Scatter: Fourier design providing up to three
measurements of forward angle
- Side Scatter: Independently focused high
performance photodiode with electronic attenuation
- Fluorescence: FL1-FL10 Fluorescent Detectors
Sample Processing
- Three selectable flow rates
- 32 tube Multi Carousel Loader or single tube mode
- Automated or manual worklist acquisition
- True Positive ID
- Biohazard contained workstation
Signal Processing
- Dynamic range: 20-bit data acquisition
- Workstation resolution: 1,048,576 channels
- Digital sampling rate: 40 Mhz
- Digital accuracy: < 5% error
Performance Characteristics
- Throughput: Up to 80 tubes/hour at 10,000 events
per second
- Scatter resolution: Resolves 0.404 m diameter
particles from background noise using forward
scatter with maximum detection of up to 40 m
diameter particles
Flow Cytometer Analyzer
Models Part # Status
Gallios 6 colors, 2 lasers (5+1 configuration) 775014
RUO
Gallios 8 colors, 2 lasers (5+3 configuration)
775015
RUO
Gallios 10 colors, 3 lasers (5+3+2 configuration)
775016
RUO
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The Navios flow cytometer provides high sensitivity and resolution as well as a wide dynamic range to run high complexity, multi-color
analyses on both clinical and clinical research samples. The Navios can be configured as either a two-laser system providing six- or
eight-color detection, or as a three-laser system offering up to 10-color analysis. The instrument can be upgraded to adapt as clinical
assays and clinical research applications become more complex.
A new optical design optimizes light collection for superior analysis of dim particles and identification of rare-event populations.
Innovative picomotor-driven beam steering optics facilitate fine adjustments of laser position from instrument-based controls. The
Navios acquisition speed of up to 25,000 events per second combines with a low data abort rate to improve data collection efficiency,
lower sample volume requirements and reduce reagent consumption. Additionally, the 20-bit digital electronics and 40-MHz pulse
sampling deliver low-end sensitivity and resolution.
Tubes in the instruments 32-position carousel are vortexed individually, which both ensures sample homogeneity and avoids over-
mixing. Automated barcode reading provides verification of carousel number, position and tube label for true positive sample
identification. Navios data files also contain cytometer settings, plots, gates and regions, providing a complete record of sample analysis.
Navios flow cytometers interface with Beckman Coulter automation systems to provide secure, high-throughput results, even at the
highest volumes. The instruments are LIS-ready, and in conjunction with Beckman Coulters FP1000 automated preparation system and
Data Innovations middleware, can provide a complete sample-in/result-out solution for clinical labs.
Navios Flow Cytometer
Instrument Part # Status
Navios 6 colors, 2 lasers (5+1 configuration) A80704
CE
Navios 8 colors, 2 lasers (5+3 configuration) A80705
CE
Navios 10 colors, 3 lasers (5+3+2 configuration) A80706
CE
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With two models to accommodate a diverse range of applications, with up to 11 standard parameters and 9 colors, the CyAn ADP
analyzer is the next step in Advanced Digital Processing with true high speed analysis capability.
Enhanced detection for flow cytometry
State-of-the-art optics and electronics
Automated compensation
Easy to use walk-up operation
CyAn ADP 7 Color has 9 parameters (FSC, SCC, FL1-FL7) and is equipped with 488 nm and 642 nm solid-state lasers. Some key
research applications include CD34 enumeration, signal transduction, antigen-specific T cell detection and multi-color
immunophenotyping. CyAn ADP 9 Color adds the 405 nm solid state laser and two fluorescent channels.
Up to 9 x 9 parameter compensation can be performed manually, using bi-exponential transformation (VisiComp) or automatically.
Additional research applications include stem cell/SP analysis, DAPI Cell Cycle analysis and CFP-YFP FRET analysis.
CyAn ADP Analyzer
CyAn ADP integrated to the HyperCyt plate loader enables
high throughput screening of 384-well plates in less than 10
minutes. With front end automation of the HyperCyt and the
sensitive optics and analysis speed of the CyAn ADP,
compound screening and HTS assays are performed quickly
and accurately.
CyAn ADP uses multiple laser excitation sources to analyze
biological cells, beads or other microscopic particles. The
CyAn ADP has state-of-the-art optics, utilizes high
performance digitization with pulse processing speed up to
70,000 events per second complementing a high acquisition
and analysis capability of up to 100,000,000 event data
files.
Instrument Configurations Part # Status
CyAn ADP, 7 Color CY20030
110V option + Starter Kit (standard kit) RUO
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Accessories Part # Status
HyperCyt Standard System A86054
RUO
HyperCyt 8 inch probe A86061
RUO
HyperCyt Ferrule- Clear Plastic A86062
RUO
HyperCyt 48 Peristaltic Tubing 18 stop A86063
RUO
HyperView Offline Experiment Designer and data Analysis A86056
RUO
HyperCyt Software Interface CyAn A86057
RUO
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The FC 500 Series delivers flexible testing, powerful software and a user-friendly interface; features that help both clinical and basic
researchers optimize their overall workflow.
The systems have the capacity to conduct 5-color analysis using either a single (488 nm) laser or a dual (488 nm/633 nm) air-cooled
laser configuration. Coupled with the ability to perform tube or plated based automated analysis, the FC 500 Series improves efficiency
by enabling users to easily manage and automate every aspect of their flow cytometric analysis.
The FC 500 incorporates Beckman Coulters ADC technology, the most advanced method of color compensation available. Combined
with high resolution acquisition electronics, its proven algorithm provides superior correction of spectral overlap for more accurate
results. With ADC technology, biological samples can be used for automated color compensation, without the limitations of latex beads.
20-bit Digital Signal Processing (DSP) on the system is responsible for excellent linearity, drift-free amplification and accurate color
compensation. High resolution color compensation is available during acquisition and in Listmode analysis, increasing the flexibility of the
system.
Both optical and digital systems work together to yield unrivalled visualization of low intensity fluorescence populations.
FC 500 Series Flow Cytometry Systems
FC 500 with Carousel loader and CXP software
Automated quality control monitoring
To comply with the rigors of the routine flow cytometry
laboratory, CXP software provides automatic daily monitoring
of system performance, simplifying daily quality control
recording. Built in Levy-Jennings plots are updated with each
QC run for constant quality monitoring. The integrated
product log keeps track of lot numbers and expiration dates
helping with compliance for regulated laboratories.
Multi-file analysis software
Data files produced by the FC 500 Flow Cytometer contain
complex biological information. Review of the data offline to
acquisition is an important process. The CXP software provides
powerful tools that offer flexibility and automation in data
analysis. It is possible to analyze multiple files concurrently or
on one desktop to glean as much information as possible from
an experiment or patient sample. Overlay histograms can be
created to visualize small changes in populations together with
three dimensional plots to analyze interacting parameters. All
of which can be processed manually or in batch mode.
Customized panel reporting
Available within CXP software to tailor reports to groups of
assays. Each report can include both user selected statistics as
well as plots from the panel. Output can be made via a printed
page or electronic PDF documents to easily e-mail to offsite
laboratories.
FC 500 MPL with MXP software
Accepts a variety of microtiter plates and 12 x 75 mm tubes.
MXPs 21 CFR part 11 tools
Provide the most complete set of optional tools to facilitate
compliance for regulated flow cytometry laboratories, giving
the maximum protection of data integrity and version tracking.
Sub systems such as inactivity timeouts and attempted failure
prevention allow only those with verified access rights to gain
entry to the software. This combined with E signatures and the
Audit Trail tracking of produced Microsoft* Excel files protects
output from the system.
MXPs plate wizard
Improves usability and simplifies plate setup, allowing the user
to rapidly create worklists for plate based analysis including
multi-well panel, protocol and sample ID assignments. For
flexible choices of sample types simply select sample volume
and mix requirements, then store plate parameters for rapid
retrieval.
Automated analysis tools for bead based assays
Multiplexed ELISA assays utilizing Micro-beads are now a
common application in flow cytometry. Software modules built
into the MXP software enable the FC 500 MPL to carry out
these assays with ease. Data from acquisition is automatically
exported to Microsoft Excel then analyzed using specifically
designed MPL analysis macros. Well plot graphs display well
data on a macro level enabling easy visualization of "hits" on
an assay by assay basis. Also included is the automatic
generation of standard curves for determination of analyte
concentration.
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Instruments Part # Status
FC 500 with Carousel Loader and CXP Software
Please call
IVD & CE
FC 500 MPL with MXP Software
Please call
IVD & CE
Red Diode Laser Upgrade 623101
-
MPL Upgrade 733321
-
tetraCXP SYSTEM Software
Please call
-
stemCXP SYSTEM Software
Please call
-
CXP Analysis Software Packages
Please call
-
Offline Analysis Workstation 773875
MultiCycle for Windows* 6415063
-
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For the first time, the Integrated Cytometry Solution offers a seamless preanalytical, analytical, and post analytical
solution coupled with complete connectivity to the Laboratory Information System (LIS). By standardizing the five major
steps a sample undergoes and managing the electronic communications between each one it delivers a progressive
automated system that simplifies and streamlines workflow processes, increases efficiency and accuracy, accelerates
throughput and minimizes operator involvement.
Integrated Cytometry Solution
RECEIVE. The sample ID, test request and demographic
information are sent from the LIS and captured in the
Instrument Manager*. Simultaneously, run information is sent
to the database on the FP 1000 Cell Preparation System.
Complex panels, protocols, reagents and procedures are
automatically set, and a worklist is generated. To ensure
accurate handling, data is transferred electronically from LIS to
final results. The whole process virtually eliminates
transcription errors and costly sample re-runs.
PREPARE. Barcode tracking on the daughter tubes provides
paperless positive ID throughout the process, the system
delivers high throughput for increased lab efficiency. By
accessing sample tubes through cap piercing as well as
automatically dispensing antibodies into the daughter tubes
technologists are protected from potential sample exposure
and reproducibility is increased. The continuous sample
feeding capability of the FP 1000 Cell Preparation System
allows for any run size to be processed smoothly and rapidly.
At the same time, the samples are tracked and verified
throughout the process, reducing errors, re-runs and incorrect
sample IDs. The user determines which reagents and lytic
system to use based on the protocol, and they are loaded into
the FP 1000. Additionally, the number of mixes can be
programmed by the user, facilitating accurate reagent setup
results, and delivering unparalleled flexibility.
ACQUIRE. With the samples properly prepared, the
carousels are moved to the FC 500. The external worklist file
from the FP 1000 is located, and opened within CXP for
processing and barcode-based sample tracking. For enhanced
flexibility the worklist can be sent via Ethernet, using native FC
500 or Excel. With its auto-setup capability, multi-color
analysis using either single or dual laser excitation, and the
ability to use a variety of dyes, the award-winning FC 500
delivers unmatched versatility.
ANALYSE. Realtime or listmode data handling, point-click
gating and 20-bit data acquisition are just a few of the ways
that the innovative FC 500 and its revolutionary CXP software
deliver accurate and easy-to-interpret results all of which
can be printed at the FC 500 or uploaded to the LIS.
REPORT. For the utmost flexibility and safety in how
information is transmitted, password-protected results can
be reviewed and verified at the Information Manager
workstation before uploading, or they can be passed on
directly to the LIS.
Sample Preparation Analyzer
Instrument Manager from Data Innovations
Please call
-
FP 1000
Please call
IVD & CE
FC 500 with CXP Software
Please call
IVD & CE
18
MoFlo XDP combines historic sorting reliability with cutting edge engineering and proven performance. The open architecture of the
instrument enables configuration modularity to meet the diverse sorting applications. The optical bench can accommodate any laser
used in Flow Cytometry. Improved light detection and filter designs provide the sensitivity required of a sorter.
The unmatched electronics ensure you identify and accurately sort each cell of interest at higher sort speeds. Accelerating cell sorting
research with the first true 32-bit-high resolution 5-decade multi-channel digital system enables accurate sorting of rare events and
stem cells. Critical for stable droplet formation required in sorting, the MoFlo XDP fluidic design is robust and proven. Whether
performing long sorts or additional precision is required for sorting into 1536-well plates, the stable fluidics of the MoFlo XDP will
facilitate your needs.
Combined with the inherent flexibility, digital processing, and fluidic stability; the intuitive software and Touch Screen Control Panel make
the set up time on a MoFlo XDP quick and easy, leaving more time for sorting critical samples.
MoFlo XDP Cell Sorter
Modularity
Compatible with any Flow Cytometry laser: water
cooled, free-space, fiber coupled
Laser Engine expands the wavelength possibilities
through a single pinhole
Fiber coupled lasers with Beam Shaping Optics
providing increased stability and alignment ease
Filter changes simplified with the Precision Optical
Detector (POD) structure
Biosafety
Certifiable Biosafety Level I or II cabinet option
Aerosol Evacuation System (AES) removes
aerosols from sort chamber
Replaceable probe and sample line
SmartSampler sample input protects the sample
and user
Electronics
Zero dead time
> 100,000 analysis events per second
Digital triggering on any parameter
Digital pulse processing
True dynamic range of 5 decades
Unmatched linearity
Sort Performance
IntelliSort II: Beadless drop delay determination
and monitoring
Validated for 70,000 sorts per second
> 99% purity at all data rates
High recovery, yield and viability
Proven 4-Way Sort for multiple population sorting
Independent Sort Mode capability in each tube
CyClone custom plate and slide sorting
Touch Screen Control Panel
Easy-to-use intuitive cytometer set up and control
Independent control of each stream
Simple coarse and fine alignment
CyClone calibration up to 1536-well plate
Real-time sort statistics
Summit Software
Established software with powerful data handling
18x18 Auto-compensation matrix
Determine regions with full parameter resolutions
> 1 Billion event listmode files
Workspace concept for flexible experiment design
Cell Sorter
MoFlo XDP
Please call
RUO
19
Leveraging the historically stable fluidic design of the original MoFlo and the electronic processing of the MoFlo XDP, the MoFlo Astrios
expands sorting capabilities with a full palette of laser options. Complex, multi-color sorting is enabled by seven pinholes that spatially
separate each of the seven lasers. The multi-fiber Beam Shaping Optic creates flat-top beam profiles, providing short alignment times
and high-level optic stability. Two stages control the alignment for all seven lasers to simplify sorter preparation.
Beyond the optic expansion, novel software components enhance the instruments capabilities. The Touch Screen Control Panel
facilitates daily set up with its operation designed around core laboratory workflow. Auto QC allows system performance to be tracked
and trended across 32 available parameters. IntelliSort II is a fully bead-less drop delay determination and monitoring system. Camera
images and proprietary algorithms enable the optimal drop delay to be determined without the use of foreign particles in the sample line.
The MoFlo Astrios also includes a new version of Summit Software containing 6-Way sorting and plate sorting up to 1536-well.
MoFlo Astrios
Optics:
7 laser / 7 pinhole configurable system; 355nm,
405nm, 488nm, 532nm, 561nm, 592nm, 642nm
Single multi-fiber Beam Shaping Optic (BSO) for fiber
coupled lasers
Flat-Top beam profile on fiber coupled lasers increases
beam stability and power, alignment ease
7 lasers aligned through two alignment stages for quick
sorter set up
Software:
IntelliSort II - The only sorter with a fully bead-less
drop delay determination and monitoring
Touch Screen Control Panel for instrument set up and
sort monitoring (intuitive operation and cross-training)
Auto Start Up enables lasers to automatically turn on
and fluidics to pressurize
Single button Auto QC for tracking and trending with
enhanced troubleshooting guidance
Summit Software version 6.0
Electronics and Software:
Electronic capability to simultaneously detect 32
parameters
32 bit processing and 100 MHz sampling speeds
Zero electronic dead time equates to no hard aborts
>100,000 acquisition rate/second validated
performance
5-decade dynamic range PMTs
FSC and SSC off each wavelength greater than or equal
to 405nm
Height, Area, Width, Log, Log Area collected
simultaneously for all parameters
Describes regions mathematically at the full resolution
of the parameter
1 billion events .fcs files with no parameter limit
Flow Cytometer Cell Sorter
20
Performance:
6-way sorting with a variety of collection devices
(temperature control for all sort outputs)
Mixed Mode sorting: each sort stream is capable of
having its own sort mode (Enrich, Purify, Single)
programmed. Soft aborts and waste can be collected
for reanalysis or resorting.
Index sorting with intuitive mapping of events
Reproducibly sort up to 1536-well plates (due to
historically stable fluidics)
Only sorter capable of >70,000 sort decisions/second
validated performance
Biosafety
Tested to NSF-49 specifications custom Biosafety Level
II cabinet option
Quick Release nozzle design for easy access to nozzle
tip
Sort chamber free of hard edges designed for aerosol
containment and clean ability
R-Theta CyClone arm with wiper (seals back of sort
chamber)
Aerosol Evacuation System (AES) pulling particulates
from three (3) chambers with verified performance to
meet the ISAC guidelines
MoFlo Astrios
Please call
RUO
21
The Beckman Coulter Vi-CELL automates the manual trypan blue viability method. The Vi-CELL removes the subjective variables
inherent in the manual assay. The instrument also reports cell concentration, size distribution, and circularity measurements. Real time
cell images may be archived for future re-analyses.
Vi-CELL
Counter
Auto Sampler Size Range Sample Volume Analysis Time
Yes 2-70 m 0.5 mL < 2.5 min
Vi-CELL XR 383556
LUO
Vi-CELL XR Single Pack 383260
Reagent Kit (includes 1 reagent pack and 1
bag of 120 sample vials per bag)
LUO
Vi-CELL XR Quad Pack 383722
Reagent Kit (includes 4 reagent packs and 8
bags of 120 sample vials per bag)
LUO
Vi-CELL Concentration Control 175478
(1 x 20 mL) LUO
Vi-Cell Focus Control 175474
(1 x 15 mL) LUO
Vi-CELL Sample Cups 383721
4 bags of 120 sample vials per bag LUO
22
The Z Series consists of three models of analyzers; the Z1 Single Threshold, the Z1 Dual Threshold, and the Z2. Each model allows for
variable, user selectable cell size settings and operates in the size range of 1-120 m diameter. Thus, the instruments have the capability of
analyzing practically all cell types and species variations.
Z Series COULTER COUNTER
Counter
Z2 Analyzer 6605700
inc. 100 m aperture LUO
Z1 Dual Threshold Analyzer 6605699
Z1 Single Threshold Analyzer 6605698
Software Part # Status
Z2 AccuComp Software 383550
LUO
(includes 200 Accuvettes ST, one Waste/Diluent sensors and the 10 mL Dispenser
Z-Series Starter Kit 8320310
LUO
(includes 3 Z-Paks)
Z-Series Tri-Pak 8320312
LUO
Accuvettes ST A35473
(200 vials with caps) LUO
N4 accuvettes 7800091
LUO
nominal 10 m, Latex Particle (NIST Traceable)
L10 Standard 6602796
(1 x 15 mL) LUO
Aperture Concentration Control 177495
(1 x 20 mL) LUO
Aperture Tube, 50 m 9912784
LUO
LUO
LUO
LUO
LUO
Aperture Tube, 50 m Ampoule 9913155
LUO
LUO
LUO
23
Whether harvesting cells, separating blood components or other general separations, you can simplify your processes and save time
with the Allegra X-15R Benchtop Centrifuge.
AllegraX-15R Benchtop Centrifuge
New power & flexibility on the bench!
Optimize Recoveries
Faster Separations
Save Time
Archive up to 5,250 xg in your 4 x 750 mL Swinging
Bucket Rotor
Some key applications Include:
Filtration and Concentration
Fast and Slow Blood Component Spins
Microbial Cell Harvesting - up to 3 liters, in as little as
14 minutes
Skip steps in cell culturing
Cell lines, primary cells, adherent cells, suspension cells
Save time, labor and a potential contamination step
Eliminate 15 mL or 50 mL conical tubes
Centrifuge cells directly in T-75 cm
2
or T-25 cm
2
flasks
Adapters currently available for Corning 75cm2 and 25cm2 canted neck
cell culture fl asks. Corning is a trademark of Corning Incorporated.
All rotors and carriers with covers certified for
biocontainment
Validation was done at CAMR, Porton Down, UK, or USAMRIID, Ft. Detrick ,MD,
USA
Complete a run Even with imbalance
ARIES Smart Balance Rotor Automatically Detects and
Corrects imbalance: up to 50 grams opposing loads
Centrifuge
Allegra X-15R Centrifuge Part # Status
208V, 60-Hz 392932
-
230V, 50-Hz 392934
-
200V, 50/60-Hz 392933
-
Canted-Neck Cell Culture Flask Adapters Part # Status
Corning* T-75 cm
2
369292
-
Corning* T-25 cm
2
369295
-
24
Highest Throughput Available6 liters in under 10 minutes.
The Avanti J-26 XP Series is designed for high throughput and versatility. Teamed with the J-LITE JLA-8.1000 6-liter rotor, the J-LITE
polycarbonate and polypropylene bottles, and disposable HarvestLine System liners, the J-26 XP centrifuges can process 6 liters in less
time than it takes conventional centrifuges to process 3 liters. These are versatile high performance systems as well, capable of running
the full line of Beckman Coulter high-performance rotorsincluding the AllSpin (JS -5.3) rotor with optional microplate carriers and
adapters for conical tubes and bottles.
Avanti J-26 XP Series High-Performance Centrifuges
Centrifuge
Avanti J-26 XPI Part # Status
200/208/240V, 50/60 Hz 393127
-
230 V, 50 Hz 393128
-
220/380 V, 50 Hz, 3-phase 393129
-
Avanti J-26 XP Part # Status
200/208/240V, 50/60 Hz 393124
-
230 V, 50 Hz 393125
-
220/380 V, 50 Hz, 3-phase 393126
-
Avanti J-26 XPI with Elutriation Part # Status
200/208/240V, 50/60 Hz 393133
-
230 V, 50 Hz 393134
-
220/380 V, 50 Hz, 3-phase 393135
-
Avanti J-26 XP with Elutriation Part # Status
200/208/240V, 50/60 Hz 393130
-
230 V, 50 Hz 393131
-
220/380 V, 50 Hz, 3-phase 393132
-
25
Biosafe Avanti J-26 XPI Part # Status
200/208/240V, 50/60 Hz A25574
-
230 V, 50 Hz A25575
-
220/380 V, 50 Hz, 3-phase A25576
-
Biosafe Avanti J-26 XP Part # Status
200/208/240V, 50/60 Hz A25577
-
230 V, 50 Hz A25578
-
220/380 V, 50 Hz, 3-phase A25579
-
26
The Optima L-XP Series Ultracentrifuges with ESP (Efficient Sedimentation Program) simulation software enables you to optimize
centrifugation protocols for applications like subcellular organelle isolations. In addition to identifying ideal run conditions for pelleting
isolations and density gradient fractionation, the simulation software assists in selecting the rotor and labware. Simulation settings can
be stored for future recall or simply downloaded and run.
Optima L-XP Series Ultracentrifuges
Centrifuge
Instruments Part # Status
Optima L-100XP 60/50 Hz, 200-240 VAC 392052
-
Optima L-100XP 50 Hz, 220-240 VAC 392050
-
Optima L-80XP 60/50 Hz, 200-240 VAC 392051
-
Optima L-80XP 50 Hz, 220-240 VAC 392049
-
Rotors Part # Status
Fixed angle rotor for pelleting isolations
Type 70.1 Ti 342184
-
Swinging bucket rotor for density gradient fractionation
SW 32 Ti 369694
-
27
Kaluza combines innovative simplicity and efficiency with unprecedented speed facilitating multiparametric data analysis.
Kaluza Analysis Software
Intelligent Workspace Design
- Analyze directly from report or plot sheet
- Drag & drop plots into a report page
- Multiple report layouts per sample
Plot Management
Unique Auto-Layout design intelligently reconfigures your
workspace for you.
- Custom plot sizing - Zoom in and out
- Easy creating of plots - Guided drag & drop
Radial Menu
Functions such as region creation and statistics selection
are a single click away.
On-plot access to:
- Creating gates - Annotating
- Formatting - Statistics
Multiparametric Plots
- Simplifies multiparametric data analysis into one
interactive display
- Enables quick pattern recognition analysis
Data Merge
- Easily combine multiple files for quality analysis
- Enables better measurement of rare populations
- Facilitates analysis of longitudinal and time course
studies
Multi-File Analysis
Analysis of multiple datasets is as simple as a drag & drop
into the intuitive analysis list. Composite analysis allows
you to quickly and easily change compensation and create
overlays with multiple files.
Software
Software Part # Status
Kaluza Software Kit Single User
A82959
RUO
Kaluza Software Kit 5-User Bundle
A82960
RUO
Kaluza Software Kit 5 User Network
A84175
RUO
Kaluza Software Kit 10 User Network
A82961
RUO
Kaluza Software Kit 1 year Single User renewable licence
Please call
RUO
28
Cell Preparation Systems
TQ-Prep Workstation & IMMUNOPREP Reagent System
The TQ-Prep Workstation and IMMUNOPREP Reagent System together provide rapid, no-wash whole-blood
sample preparation. Automation provides inter-sample and inter-laboratory consistency and reproducibility. A
standard 32-tube carousel allows walk-away processing and perfectly complements both the XL-MCL and FC
500 flow cytometers automatic sample loaders for the fastest sample throughput in the industry. Intended
primarily for laboratories with a high test volume (at least 300 tests per month), the TQ-Prep uses the
revolutionary patented lyse and fix technology introduced more than a decade ago with the Q-PREP
Workstation and IMMUNOPREP Reagent System.
Size Description Status Part #
7546999 IVD & CE 300 tests IMMUNOPREP
TQ-Prep Workstation
6605429 IVD & CE 100-240 Vac Standard 50/60 Hz
6915115 IVD - With LALS Option
29
PrepPlus 2 Workstation
The PrepPlus 2 provides precision pipetting of reagents, patient samples, controls and fluorospheres into
daughter tubes of both the XL-MCL and FC 500 Carousels. Lysing is then automatically performed on the TQ-
Prep. Other features include:
- Flexible software programming of reagents, controls and pipetting parameters
- Improved safety with closed tube sampling
- Standardized, reproducible performance
PrepPlus 2 Accessory Kit
6915549 - - Accessory Kit
378600 IVD & CE - Workstation
30
Biomek3000 Laboratory Automation Workstation
By integrating all aspects of liquid handling into a single, automated system, the Biomek 3000 delivers
unparalleled flexibility, expandability and performance. Options like side modules for increased capacity and
interchangeable tools ensure that all your assay needs are efficiently handled. And a small footprint lets it fit
inside laminar flow hoods. Plus, our ongoing applications development including eLabNotebook online
offers pre-programmed automated solutions for a wide variety of applications.
The Biomek 3000s ability to perform easy system modifications is just another way our flexible system
design helps you meet expanding research requirements. For example, applications like DNA purification
from whole blood benefit by going from single-channel tube pipetting to multichannel plate pipetting within
the same automated method.
Choose from a variety of add-ons to customize your liquid handling application needs, and integrate them
easily with Biomek Software. For applications like PCR* purification, you can increase throughput by adding a
stacker carousel, or enhance functionality with a DPC Micromix shaker.
If you have special requirements, our custom engineering group can create a particular solution for you.
Biomek 3000
Please call
- - Workstation
31
BiomekNX
P
Laboratory Automation Workstation
By incorporating a wide range of next-generation features into a small footprint design, Biomek NX
P
sets the
standard for flexible laboratory solutions. It puts every aspect of liquid handling including pipetting dilution,
dispensing and integration into a single, automated system that is as powerful and flexible as it is efficient
and economical. All while offering unprecedented flexibility for changing needs. Combined with robust Biomek
Software and our ongoing applications development, Biomek NX
P
is bringing new possibilities to life.
The flexible, open architecture of Biomek NX
P
simplifies integration, as plate readers,washers and storage
devices can be located on the left, right or back of the instrument. The open frame design allows future
vertical workspace integration, for applications where limited lab space is an issue.
The mouse-driven simplicity of our unique point-and-click programming avoids the long learning curve so
common to other approaches. Alternatives such as pipetting techniques, liquid types and deck layouts can be
chosen from drop-down lists.Method validation features will check your method and find any logical errors.
The Estimated Time to Completion (ETC) feature will provide you with an accurate run time, enabling you to
speed up methods development and efficiently plan your instrument use.Variables, worklists and logicals can
be used to vary runs without reprogramming. All of which makes a wide variety of applications from nucleic
acid purification to cell-based assays extraordinarily efficient and streamlined.
Biomek NX
P
Please call
- 100-240 Vac Power Configuration 50/60 Hz
32
FP 1000 Cell Preparation System
The FP 1000 Cell Preparation System is available as a stand alone system or as part of the Beckman Coulter
Integrated Cytometry Solution.
Using both pre-programmed and user defined methodologies, the system is capable of pipetting whole blood
and staining reagents into daughter tubes. The FP 1000 can then perform RBC lysis and cell fixation on each
daughter tube utilizing the IMMUNOPREP Reagent System or other lytic reagents such as VersaLyse and
IOTest 3 Lyse.
Rapid throughput on the system is achieved by many on-
board subsystems:
Primary samples are continuously loaded utilizing tube
racks derived from Beckman Coulter high throughput
hematology analyzers.
A dual arm robotic system is used for both simultaneous
reagent addition and internal carousel transfer.
For rapid throughput in batch mode preparation, two
integrated TQ-Prep workstations have been incorporated.
The system has a total capacity of three 32 tube
carousels. This includes an input-output area for 3
carousels which can be loaded and unloaded without
stopping reagent pipetting and sample preparation.
Patient ID tracking is totally preserved with the system. The
primary sample tube is scanned for the sample ID, this ID
is then compared to a list of exact sample IDs downloaded
onto the system at the FP 1000 workstation or via test
request from the optional Laboratory Information System
connection. The appropriate sample preparation
methodology and reagent panel is then automatically
selected and the number of tubes required is defined by the
system. Prelabeled daughter tubes are then scanned and
allocated to the original sample ID. These are then used in
preparation of the flow cytometry worklist and tracked
throughout the run.
Once prepared, the samples are ready for analysis on a
flow cytometer. Worklists prepared by the FP 1000 are
compatible with both Beckman Coulter FC 500 and EPICS
XL Series Flow Cytometers equipped with current software.
624922 IVD & CE - Workstation
33
Fluorospheres and Quality Controls
APC (675/633) Set-up Kit
The APC (675/633) Setup Kit is a set of fluorospheres (fluorescent microbeads) used for daily verification of a
flow cytometers optical alignment and fluidic stability and as an aid in standardizing the detectors on a flow
cytometer for the quantitative analysis of human leukocytes. Data from each fluorosphere is collected with the
675 nm detector when excited with a 633 nm light source. The kit is comprised of two vials: one vial of Flow-
Check 675 fluorospheres and one vial of Flow-Set 675 fluorospheres.
Flow-Set 675 Fluorospheres 1 x 10 mL 1 vial
Flow-Check 675 Fluorospheres 1 x 10 mL 1 vial
Size Presentation
Status Part #
737663 CE
6607120 RUO
Flow-Check Fluorospheres
Flow-Check Fluorospheres consist of 10 m (nominal diameter) polystyrene fluorescent microspheres
suspended in an aqueous medium containing surfactants and preservatives at 1 x 10
6
fluorospheres/mL
(nominal concentration). The fluorescence emission of the dye contained within the fluorospheres ranges from
525 nm to 700 nm when excited at 488 nm. Flow-Check Fluorospheres are used to verify instrument optical
alignment and fluidics.
Fluorospheres (10 m) 10 mL/vial 3 vials
Size Presentation
Status Part #
6605359 IVD & CE
Flow-Check Pro Fluorospheres
Flow-Check Pro Fluorospheres is a suspension of fluorescent microspheres which may be used for daily
verification of optical alignment and fluidics of flow cytometer's configured with multiple lasers (Blue, Red, and
Violet). The Fluorosphere suspension consists of a mixture of 10 m fluorescent microbeads with a
fluorescence emission of 515 nm to 800 nm when excited at 488 nm, 6 m fluorospheres with a fluorescence
emission of 640 nm to 800 nm when excited at 635 nm, and 3 m fluorospheres with a fluorescence emission
of 400 nm to 500 nm when excited at 405 nm, respectively.
Fluorospheres (10 m; 6 m; 3 m) 10 mL/vial 3 vials
Size Presentation
Status Part #
A63493 CE
A69183 RUO
34
Flow-Count Fluorospheres
Flow-Count Fluorospheres are a suspension of fluorescent microbeads used to determine absolute counts on
the flow cytometer. Each fluorosphere contains a dye which has a fluorescent emission range of 525 nm to 700
nm when excited at 488 nm. They have uniform size and fluorescence intensity, and an assayed concentration
allowing a direct determination of absolute counts.
Fluorospheres (10 m) 20 mL/vial 1 vial
Size Presentation
Status Part #
7547053 IVD & CE
Flow-Set Fluorospheres
Flow-Set Fluorospheres are a suspension of fluorospheres (fluorescent microbeads) used as an aid in
optimizing a flow cytometer for quantitative analysis of human leukocytes. Each fluorosphere contains a dye
which has a fluorescent emission range of 525 nm to 700 nm when excited at 488 nm. They have a uniform
size and fluorescence intensity allowing the standardization of light scatter, fluorescence intensity and optimal
hydrodynamic focusing instrument settings.
Fluorospheres (3.6 m) 10 mL/vial 3 vials
Size Presentation
Status Part #
6607007 IVD & CE
Flow-Set Pro Fluorospheres
Flow-Set Pro Fluorospheres is a suspension of fluorescent microspheres which may be used as an aid in
standardizing a flow cytometer configured with multiple lasers (Blue, Red, and Violet). The suspension contains
3 m (nominal diameter) polystyrene fluorospheres suspended in an aqueous medium containing surfactants
and preservatives.
Size Presentation
Status Part #
A63492 CE
A69184 RUO
IMMUNO-BRITE Fluorospheres
IMMUNO-BRITE Fluorospheres are a suspension of fluorescent microbeads uniform in size with varying
fluorescence intensities. The set contains one vial of non-fluorescent fluorospheres and four vials of
fluorospheres of increasing intensity. They may be used to monitor instrument linearity.
Fluorospheres 10 mL/vial 5 vials
Size Presentation
Status Part #
6603473 IVD & CE
35
PC7 (770/488) Set-up Kit
The PC7 (770/488) Setup Kit is used for daily verification of a flow cytometers optical alignment and fluidic
stability and as an aid in standardizing the detectors on a flow cytometer for the quantitative analysis of
human leukocytes. Data from each fluorosphere is collected with the 770 nm detector when excited with a 488
nm light source. The kit is comprised of two vials: one vial of Flow-Check 770 fluorospheres and one vial of
Flow-Set 770 fluorospheres.
Flow-Set 770 Fluorospheres 1 x 10 mL 1 vial
Flow-Check 770 Fluorospheres 1 x 10 mL 1 vial
Size Presentation
Status Part #
737664 CE
6607121 RUO
7-AAD Viability Dye
7-AAD Viability Dye is a ready-to-use reagent allowing discrimination of viable from non viable cells using flow
cytometry. It is composed of 7-Amino-Actinomycin D (7-AAD) which intercalates between cytosine and guanine
bases of the DNA. The maximum absorption of the 7-AAD / DNA complex is situated in the green spectral
region, compatible with argon laser equipped-cytometer (excitation wavelength of 488 nm). The deep red
fluorescence emission of 7-AAD (635 to 675 nm) makes the use of this probe easier in combination with
fluorescein isothiocyanate (FITC) and phycoerythrin (PE) conjugated antibodies because, in contrast to
propidium iodide (PI), the 7-AAD / DNA complex shows minimal spectral overlap with FITC and PE.
Viability Dye 150 tests 1 vial
Size Presentation
Status Part #
A07704 CE
IM3422 RUO
CYTO-COMP Cell Kit
CYTO-COMP Cell Kit is a preparation of lyophilized human lymphocytes and reconstitution buffer used in
conjunction with the CYTO-COMP Reagent Kit to establish and monitor color compensation settings for
multicolor analysis.
Cells ( 3.5 x 10
6
cells/vial)
50 tests 5 vials
Reconstitution buffer 1 mL/vial 5 vials
Size Presentation
Status Part #
6607023 IVD & CE
36
CYTO-COMP Reagent Kit
CYTO-COMP Reagent Kit is comprised of four sets of two-color reagents used in conjunction with the CYTO-
COMP Cell Kit to establish and monitor color compensation settings for multicolor analysis.
COMP 1 (FITC/PE) 50 tests 1 vial
COMP 2 (PE/ECD) 50 tests 1 vial
COMP 3 (PE/PC5) 50 tests 1 vial
COMP 4 (ECD/PC5) 50 tests 1 vial
Size Presentation
Status Part #
6607021 IVD & CE
CYTO-TROL Control Cells
CYTO-TROL Control Cells are used to assess the activity of monoclonal antibodies by flow cytometry. They are
a lyophilized preparation of human lymphocytes that exhibit surface antigens detectable with monoclonal
antibodies. These cells are isolated from peripheral blood and express antigens that are representative of those
found on normal lymphocytes. They may be used with directly-conjugated monoclonal antibodies.
Cells ( 3.5 x 10
6
cells/vial)
50 tests 5 vials
Size Presentation
Status Part #
6604248 IVD & CE
IMMUNO-TROL Cells
IMMUNO-TROL Cells are a positive process control for flow cytometry. They are assayed for lymphocyte,
granulocyte and monocyte specific antigens and single platform absolute counts. Light scatter, population
distribution, fluorescence intensity, and antigen density mimic those of whole blood.
Quality Control Product 60 tests 2 x 3 mL
Size Presentation
Status Part #
6607077 IVD & CE
IMMUNO-TROL Low Cells
IMMUNO-TROL Low Cells provide the same quality and reproducibility as IMMUNO-TROL Cells in a quality
control product for low-level CD4 counts. IMMUNO-TROL Low Cells also provide open vial stability of 90 days
for decreased waste.
Size Presentation
Status Part #
6607098 IVD & CE
37
QuickCOMP 2 Kit
The QuickCOMP 2 Kit consists of two single-color fluorescent reagents comprised of one monoclonal antibody
each. Each antibody is labeled with either FITC or PE. It may be used with the CYTO-COMP Cell Kit to adjust
color compensation settings on a flow cytometer equipped with AutoSetup Software.
CD45-FITC 50 tests 1 vial
CD45-PE 50 tests 1 vial
Size Presentation
Status Part #
177018 IVD & CE
QuickCOMP 4 Kit
The QuickCOMP 4 Kit consists of four single-color fluorescent reagents comprised of one monoclonal antibody
each. Each antibody is labeled with one or four fluorochromes: FITC, PE, ECD or PC5. It may be used with the
CYTO-COMP Cell Kit to adjust color compensation settings on a flow cytometer equipped with AutoSetup
Software.
CD45-ECD 50 tests 1 vial
CD45-PC5 50 tests 1 vial
Size Presentation
Status Part #
177017 IVD & CE
Stem-Trol Control Cells
COULTER Stem-Trol Control Cells are preserved KG-1a cells, that have been modified and stabilized (patent
pending) to express the CD34 Class III epitopes and the CD45 leucocyte common antigen at densities that
approximate normal human hematopoietic progenitor cells (HPC). This product is a ready-to-use cell
suspension. COULTER Stem-Trol Control Cells provide an antibody-to-antigen positive control for CD34 and
CD45 staining in flow cytometry studies. The concentration of this cell suspension is precisely calibrated.
Therefore it is also designed as an internal control for absolute count verification procedures in CD34
+
HPC
absolute counting process.
HPC Control Cells 0.2 mL/vial 10 tests 1 vial
Size Presentation
Status Part #
IM3632 IVD & CE
38
Stem-Comp Reagent
The Stem-Comp Reagent consists of a two color fluorescent reagent implementing two monoclonal antibodies.
The antibodies are conjugated to fluorescein isothiocyanate (FITC) for the fluorescence 1 channel (FL1) of a
flow cytometer and R Phycoerythrin (PE) for the fluorescence 2 channel (FL2). The Stem-Comp Reagent is
used to adjust color compensations for automated or manual analysis with the Stem-Kit Reagents (Part#
IM3630).
Stem-Comp Reagent 50 tests 1 vial
Size Presentation
Status Part #
IM3631 IVD & CE
39
Lysing, Permeabilizing and Fixative Solutions
Status Part #
Size
IVD & CE 7546946 For Q-PREP Workstation 100 tests
IVD & CE 7546999 For TQ-Prep Workstation 300 tests
The IMMUNOPREP Reagent System is a rapid, no-wash, standardized, whole blood preparation procedure
maximizing sample to sample, and laboratory to laboratory reproducibility. The IMMUNOPREP reagent lysing
action reduces cell stress and minimizes cell shrinkage while maintaining cellular integrity and morphology.
Separation of leukocyte cell populations of interest from red blood cell ghosts and debris is maximized. Scatter
characteristics are optimized for superior cell type resolution. The IMMUNOPREP Reagent Kit is comprised of 3
ready-to-use reagents:
- Reagent A lyses the red blood cells.
- Reagent B buffers the solution and stops the lysing process.
- Reagent C fixes the cells.
Status Part #
IntraPrep Permeabilization Reagent
Size
IVD & CE A07802 Fixative + Permeabilizer
(5 mL + 5 mL)
50 tests
IVD & CE A07803 Fixative + Permeabilizer
(15 mL + 15 mL)
150 tests
RUO IM2388 Fixative + Permeabilizer
(5 mL + 5 mL)
50 tests
RUO IM2389 Fixative + Permeabilizer
(15 mL + 15 mL)
150 tests
IntraPrep Permeabilization Reagent is a set of two ready-to-use reagents designed for the immunological
detection of intracellular leucocyte antigens by flow cytometry. It uses a formaldehyde / saponin-based
permeabilization procedure. IntraPrep Permeabilization Reagent can be used for detection of most cytoplasmic
and nuclear antigens in whole blood samples. It creates apertures in the membrane without affecting the gross
morphology of the cell. The resolution of each cell type on a dual light scatter histogram is well preserved
during processing with this reagent system. The standard procedure consists of first, fixing cells with Reagent
1, and second, permeabilizing the white cells and lysing red cells using Reagent 2. The reagent formulation is
optimized to minimize non-specific intracellular staining. The IntraPrep Permeabilization Reagent can be used
for simultaneous detection of cell surface and cytoplasmic antigens by flow cytometry.
40
Status Part #
IOTest 3 Lysing Solution
Size
IVD & CE A07799 Lysing Solution 100 tests
RUO IM3514 Lysing Solution 100 tests
IOTest3 Lysing Solution is an ammonium chloride-based erythrocyte lysing solution designed for flow
cytometry. This reagent is provided as a 10X-concentrated mixture of ammonium chloride, potassium
bicarbonate and ethylenediamine tetraacetic acid (EDTA). IOTest3 Lysing Solution allows whole blood or bone
marrow specimen preparation by erythrolysis during immunophenotyping procedures. A wash post lysis is
required to yield optimal results.
This dual-parameter light scatter
histogram illustrates the expected spatial
separation of leucocyte subsets after red
cell lysis of normal peripheral blood
with IOTest3 Lysing Solution and
fixation with IOTest3 Fixative Solution.
Status Part #
IOTest 3 Fixative Solution
Size
IVD & CE A07800 Fixative Solution 100 - 200
RUO IM3515 Fixative Solution 100 - 200
The IOTest3 Fixative Solution allows whole blood or bone marrow specimen preparation by fixing the cell
suspension after erythrolysis during immunophenotyping procedures. At least one wash is required between the
lysis and the fixation steps. The IOTest3 Fixative Solution is optimized to work in combination with the IOTest3
Lysing Solution. IOTest Fixative Solution is a formaldehyde-based fixative solution designed for flow cytometry.
This reagent is provided as a concentrated solution in PBS.
41
Status Part #
OptiLyse No-Wash Lysing Solutions
Size
IVD & CE A11895 OptiLyse C 200 tests
RUO A11894 OptiLyse C 200 tests
RUO IM1400 OptiLyse B 250 tests
Both OptiLyse C and B are erythrolytic reagents intended for lysing human red blood cells following direct
immunofluorescence staining of whole blood for flow cytometry. OptiLyse C is a ready-to-use lysing solution
optimized for use on Beckman Coulter instruments. OptiLyse B is a ready-to-use lysing solution optimized for
use on BD Biosciences* instruments.
This dual-parameter light scatter
histogram illustrates the expected spatial
separation of leucocyte subsets after red
blood cell lysis of normal peripheral
blood with OptiLyse C.
Status Part #
PerFix-nc
Size
RUO B10825 Fixative Buffer : 1 vial
Permeabilizing Buffer : 1 vial
Final 10X Solution : 1 vial
75 tests
The PerFix-nc Kit (no centrifuge assay Kit) has been developed to enhance the signal-to-noise ratio of
intracellular staining and simplify the workload necessary for the sample preparation.
Accurate detection of both intracellular and extracellular epitopes are obtained, while:
There are no washing steps through the whole STRAIGHT procedure (only a final wash step is described as
optional).
Several surface markers can be added together with the intracellular markers and incubated simultaneously
during the permeabilization step.
Total duration of the procedure and total workload are similar to current procedures for surface staining (e.g.
VersaLyse + Fix No Wash), and much shorter than common permeabilization procedures.
Potential automation of the PerFix-nc STRAIGHT procedure is rendered possible thanks to the removal of the
washing steps.
Illustration of leucocyte pattern and
intracytoplasmic B-Cell staining on
normal sample, implementing the
PerFix-nc STRAIGHT protocol.
ZAP-70-PE staining on lymphocytes
of another normal sample,
implementing the PerFix-nc + wash
protocol.
42
Status Part #
PerFix-p
Size
RUO B08488 Fixation Buffer: 1 vial
Lysis Buffer: 2 vials
Resuspension Buffer: 1 vial
50 tests
The PerFix-p Kit provides a simple sample preparation protocol to fix and permeabilize cells for the labelling and
visualization of multiple intracellular phosphoepitopes by flow cytometry. The PerFix-p Kit also allows the
combined labelling of both intracellular phosphoepitope and cell surface markers.
Status Part #
ThromboFix Platelet Stabilizer
Size
RUO 6607131 Platelet Stabilizer 25 tests
RUO 6607130 Platelet Stabilizer 100 tests
The ThromboFix Platelet stabilizer extends the window for platelet analysis by ensuring that the platelets
cannot be induced to a new functional state, for example, activated. Platelet Stabilizer preserves platelets in
their current state at the time of preparation. The ThromboFix Platelet stabilizer may be used in conjunction
with platelet monoclonal antibody-dye conjugates for flow cytometric analysis.
Status Part #
VersaLyse Lysing Solution
Size
IVD & CE A09777 Lysing Solution 100 tests
RUO IM3648 Lysing Solution 100 tests
VersaLyse Lysing Solution is a reagent used to lyse red blood cells from any biological fluid and, in particular, to
lyse erythrocytes from whole blood. VersaLyse is a highly specific, very gentle lysing solution, suitable for
applications such as immunophenotyping, cell sorting, activation or cell culture. It is designed for use in flow
cytometry and facilitates obtaining highly discriminative scattergrams whatever the type of flow cytometer
used. VersaLyse is a versatile reagent which may be used in lyse / wash, wash / lyse and lyse / no-wash
procedures with undiluted or diluted samples.
The histograms are dual-parameter light scatter histograms (FS versus SS) of a normal K
3
EDTA anticoagulated
blood sample lysed with VersaLyse Lysing Solution (No-wash procedure).
Acquisition with a FC 500 flow
cytometer using CXP Analysis software.
Acquisition with a BD Biosciences
FACSCalibur* flow cytometer equipped
with CellQuest* acquisition Software.
Analysis utilizing EXPO32 analysis
Software.
43
Status Part #
Whole Blood Lysing Reagents
Size
IVD & CE 6602764 Lysing Kit 100 tests
IVD & CE 6603152 Lysing Kit 300 tests
The 100-test lysing kit (part number 6602764) provides Immuno-Lyse, Fixative and Stabilizer for preparation
of whole blood for analysis by fluorescence microscopy or flow cytrometry. The 300-test lysing kit (part number
6603152) provides Immuno-Lyse and Fixative for preparation of whole blood for analysis by flow cytometry
only.
44
Systems and Kits
tetraCXP SYSTEM
The tetraCXP SYSTEM provides a fully automated
Windows* based device for enumeration of CD3
+
CD4
+
and
CD3
+
CD8
+
lymphocytes from whole blood in a single tube.
This system, exclusively available on the FC 500 flow
cytometer with CXP Software, consists of a set of reagents
and the tetraCXP Software which features:
- all protocols required for cytometer set-up,
standardization and verification for 4-color analysis.
- automated algorithm combines information from 4
parameters (Forward Scatter, Side Scatter and CD45 and
CD3) to generate a 3 dimensional lymphocyte gate.
- direct display of total CD3
+
, CD3
+
CD4
+
, CD3
+
CD8
+
,
CD19
+
and CD3
-
CD56
+
percentages and absolute counts
as well as CD4/CD8 ratio and percentage of total
lymphocytes in real time.
- automated cursor placement allows analysis of
lymphocyte components.
Status Part # Size
CD45-FITC/CD4-PE/CD8-ECD/CD3-PC5
6607013 IVD & CE 50 tests
6605359 IVD & CE 10 mL/vial
CYTO-COMP Cell Kit
Cells ( 3.5 x 10
6
cells/vial)
50 tests 5 vials
QuickCOMP 4 Kit
CD45-ECD 50 tests 1 vial
CD45-PC5 50 tests 1 vial
7547053 IVD & CE
6604248 IVD & CE
Cells ( 3.5 x 10
6
cells/vial)
50 tests 5 vials
IMMUNO-TROL Cells
6607077 IVD & CE
6607098 IVD & CE
Please call
- -
45
Systems and Kits
tetraONE SYSTEM
The tetraONE SYSTEM provides a fully-automated device
for enumeration of CD3+CD4+ and CD3+CD8+
lymphocytes from whole blood in a single tube. This
system, exclusively available on COULTER EPICS XL/XL-
MCL 4-color flow cytometers, consists of several reagents,
listed below, and the tetraONE Software which features:
- all protocols required for cytometer set-up,
standardization and verification for 4-color analysis.
- 3 dimensional lymphocyte auto-gating using Forward
Scatter, Side Scatter and CD45.
- direct display of total CD3+, CD3+CD4+, CD3+CD8+,
CD19+ and CD3-CD56+ percentages and absolute counts
as well as CD4/CD8 ratio and percentage of total
lymphocytes in real time.
- cellStat 3D Algorithm, a proprietary curve-fitting
algorithm for the determination of cursor positions for
automated population analysis.
Status Part # Size
CYTO-COMP Cell Kit
Cells ( 3.5 x 10
6
cells/vial)
50 tests 5 vials
COMP 1 (FITC/PE) 50 tests 1 vial
COMP 2 (PE/ECD) 50 tests 1 vial
COMP 3 (PE/PC5) 50 tests 1 vial
COMP 4 (ECD/PC5) 50 tests 1 vial
7547053 IVD & CE
6604248 IVD & CE
Cells ( 3.5 x 10
6
cells/vial)
50 tests 5 vials
IMMUNO-TROL Cells
6607077 IVD & CE
6607098 IVD & CE
tetraONE SYSTEM Software
Please call
- -
46
Systems and Kits
Stem-Kit Reagents
Status Part # Size
Stem-Kit Reagents
IM3630 IVD & CE 50 tests
CD45-FITC/CD34-PE 50 tests
CD45-FITC/IsoClonic Control-PE 50 tests
7-AAD Viability Dye 1 vial
10X NH
4
Cl Lysing Solution 2 vials
Stem-Count Fluorospheres 1 vial
stemCXP SYSTEM
The stemCXP SYSTEM provides the first fully automated
Windows*-based device for the simultaneous identification
and enumeration of CD45
+
and CD45
+
/CD34
+
hematopoietic progenitor cell (HPC) populations from
fresh peripheral or mobilized peripheral whole blood, fresh
bone marrow, fresh and thawed apheresis products and
cord blood. The stemCXP SYSTEM follows the ISCT
(International Society for Cellular Therapy, formerly
ISHAGE) guidelines.
The stemCXP Software provides all protocols required for
automated standardization of light scatter and
fluorescence intensities, and automated adjustment of
color compensation settings.
Status Part # Size
CYTO-COMP Cell Kit
Cells ( 3.5 x 10
6
cells/vial)
50 tests 5 vials
Stem-Kit Reagents
10X NH
4
Please call
- -
47
Systems and Kits
stemONE SYSTEM
The stemONE SYSTEM provides a fully automated device
for the simultaneous identification and enumeration of
CD45+ and CD45+/CD34+ hematopoietic progenitor cell
(HPC) populations from fresh peripheral or mobilized
peripheral whole blood, fresh bone marrow, fresh and
thawed apheresis products and cord blood. The stemONE
SYSTEM follows the ISCT (International Society for
Cellular Therapy, formerly ISHAGE) guidelines. This
SYSTEM is exclusively available on the COULTER EPICS
XL/XL-MCL Flow Cytometer with System II software.
The stemONE Software provides all protocols required for
automated standardization of light scatter and
fluorescence intensities, and automated adjustment of
color compensation settings.
Status Part # Size
CYTO-COMP Cell Kit
Cells ( 3.5 x 10
6
cells/vial)
50 tests 5 vials
Stem-Comp Reagent
Stem-Comp Reagent 50 tests 1 vial
HPC Control Cells 0.2 mL/vial 10 tests 1 vial
Stem-Kit Reagents
10X NH
4
stemONE SYSTEM Software
Please call
- -
48
Systems and Kits
reticONE SYSTEM
Status Part # Size
The reticONE SYSTEM offers a fully-automated device for enumeration of reticulocytes from whole blood in a single tube. This
system, exclusively available on COULTER EPICS XL/XL-MCL 3- and 4-color flow cytometers, consists of:
- reticONE Reagent Kit comprised of Retic-CAL Biological Calibrator and Retic-STAT Dye.
The Retic-CAL is used for instrument standardization.
The Retic-STAT Dye is a metachromatic dye that labels reticulocytes and permits interfering substances, such as white blood
cells and platelets to be excluded from the reticulocyte calculation.
- the reticONE SYSTEM Software, with all protocols required for cytometer setup and standardization. This software includes
two additional research protocols for allowing measurement of Cell Maturity Index (CMI) and Reticulocyte Distribution Index
(RDI).
The 3-color COULTER EPICS XL/XL-MCL requires a 675 BP filter.
Filter, 675 BP
6915056 - 1 unit
For 3-color EPICS XL/XL-MCL
reticONE Reagent Kit
7547059 IVD 100 tests
Retic-CAL Biological calibrator 100 tests
Retic-STAT Dye 100 tests
reticONE SYSTEM Software
6915048 - 1 unit
COULTERManual CD4 Count Kit
Status Part # Size
The COULTER Manual CD4 Count Kit is a portable, low-cost CD4 count method for determining the immune status of patients
without the use of a flow cytometer. It involves latex beads coated with specific monoclonal antibodies. The antibody coated
latex beads form rosettes around the cells of interest. The COULTER Manual CD4 Count Kit provides 2 m diameter beads
coated with monoclonal antibody to CD4 antigen (COULTER CD4 Cyto-Spheres Reagent ), 0.2 m diameter beads coated
with monoclonal antibody to CD14 antigen (COULTER MY4 Cyto-Spheres Monocyte Blocking Reagent) used as a monocyte
blocking reagent and a vial of COULTER Lysing Reagent. This simple, 10 minute method correlates with results from a flow
cytometer to 30 cells/L. For light microscopy.
CD4 Count Kit
CD4 Cyto-Spheres Reagent 0.5 mL Liquid
MY4 Cyto-Spheres Reagent 0.5 mL Liquid
Lysing reagent 5 mL Liquid
49
Systems and Kits
Allergenicity Kit - Cellular Analysis of Allergy
Allergenicity Kit, Cellular Analysis of Allergy consists of an
optimized three-color combination of fluorescent
monoclonal antibodies, an activation solution, a positive
control for IgE-mediated basophil activation, a stop
solution, a lysing solution and a fixative solution.
The Allergenicity Kit is intended for the identification of
resting and activated basophils based on an accurate
basophils gating tool (utilizing CRTH2
pos
CD203c
pos
CD3
neg
).
The analysis is performed on whole blood specimens. The
cell population of interest is stained with monoclonal
antibodies in the presence of the allergen or controls.
Erythrocytes are then lysed prior to flow cytometry
analysis. Once T lymphocytes (CD3
+
cells) are excluded,
basophils are identified using CRTH2 and CD203c
expression. Non-activated and resting basophils are
CRTH2
pos
CD203c
dim
CD3
neg
, whereas in vitro activated
basophils are CRTH2
pos
CD203c
bright
CD3
neg
.
The flow cytometer must be equipped to detect forward
scatter, side scatter and three fluorescence channels
allowing the analysis of FITC-, PE-, and PC7- conjugated
antibodies.
Status Part # Size
Allergenicity Kit
A17116 CE 100 tests
CRTH2-FITC / CD203c-PE / CD3-PC7 2 mL 1 vial
Positive Control 0.2 mg 1 vial
Activation Solution 5 mL/vial 2 vials
Stop Solution 10 mL 1 vial
Lysing Solution 100 mL/vial 3 vials
Fixative Solution 10 mL 1 vial
EPICS XL PC7 Filter Kit
6915493 - -
Needed to run the Allergenicity Kit on the EPICS XL Series
50
Systems and Kits
Annexin V Kits
In early apoptosis, cells lose membrane phospholipid
asymmetry, whilst the integrity of the cell membrane is
maintained. The Annexin-V kits contain a viability dye to
evaluate membrane integrity as well as annexin-V to
assess membrane asymmetry.
In fact, phosphatidylserine (PS), a negatively charged
phospholipid located in the inner surface of the plasma
membrane, appears very early in apoptosis at the
external cell surface. Annexin V, a calcium and
phospholipid binding protein, binds preferentially to PS.
The Annexin V kits employ recombinant annexin V labeled
with biotin, fluorescein or R-phycoerythrin in together with
the viability dyes propidium iodide (PI) or 7-amino
actinomycin D (7-AAD).
Apoptotic cells are easily identified utilizing a rapid and
simple staining procedure. Fixation of the cells is not
necessary.
When cells are double-stained with annexin V and a
viability dye, three different cell populations may be
observed (see figure below): (i) live cells that do not stain
either with annexin V or with the vital dye, (ii) necrotic or
late apoptotic cells that stain with both annexin V and the
vital dye, and (iii) early apoptotic cells that stain with
annexin V only.
Analysis may be performed on any flow cytometer
equipped with a 488nm laser or Arc Lamp depending on
the reagent kit chosen. Alternatively, the cells may be
examined with a fluorescent microscope or cell imaging
system equipped with the appropriate filters.
Status Part # Size
Annexin V-Biotin Kit (20 tests)
IM2585 RUO 20 tests
Annexin V-Biotin 1 vial - ready-to-use
Binding Buffer 2 vials - 10X concentrated
Propidium Iodide 1 vial of powder
Annexin V-Biotin Kit (200 tests)
IM2586 RUO 200 tests
Annexin V-Biotin 1 vial - ready-to-use
51
Systems and Kits
Annexin V-FITC Kit (20 tests)
IM2375 RUO 20 tests
Annexin V-FITC 1 vial - ready-to-use
Binding Buffer 1 vial - 10X concentrated
Annexin V-FITC Kit (200 tests)
Annexin V-FITC 1 vial - ready-to-use
Annexin V-FITC/7-AAD kit
Annexin V-FITC 1 vial
7-AAD 1 vial
ApoScreen* Annexin V Apoptosis Kit-FITC
731718 RUO 100 tests
Recombinant Annexin V-FITC 1 mL
Propidium Iodide 1 mL
Binding Buffer 7 x 1.5 mL
ApoScreen* Annexin V Apoptosis Kit-PE
736518 RUO 100 tests
Recombinant Annexin V-PE 1 mL
7-AAD 1 mL
Binding Buffer 7 x 1.5 mL
52
Systems and Kits
Residual Leukocyte Enumeration
Status Part # Size
The LeukoSure Enumeration Kit is comprised of a set of reagents used to prepare and enumerate white blood cells in
leukoreduced red blood cell and platelet products by flow cytometry. The LeukoSure Enumeration Kit is configured to utilize
the sensitivity of flow cytometry to enumerate residual leukocytes at the levels necessary to ensure the quality control of
leukoreduced blood.
The enumeration method depends upon mixing a known volume (100 L) of LeukoSure Fluorospheres with an identical
volume of sample to be tested. After analysis the absolute count for the specimen is calculated. This represents the absolute
number of leukocytes in the specimen.
The specimen is lysed and permeabilized using the LeukoSure Lyse Reagent to eliminate RBCs and prepare the cells for
subsequent addition of the LeukoSure Stain Reagent which contains propidium iodide and RNAse (enzyme used to remove
ribonucleic acid, or RNA). In the absence of RNA, propidium iodide binds only to double stranded deoxyribonucleic acid (DNA)
so that nucleated cells in the sample emit fluorescence in proportion to their DNA content. The flow cytometer measures the
fluorescence from each stained cell as it passes through the laser beam. Since mature platelets and red blood cells do not
contain DNA, the stained cells represent the leukocyte component of the blood.
LeukoSure Enumeration Kit
Leuko-Trol Platelet Control Kit
Leuko-Trol RBC Control Kit
TdT
Status Part # Size
Terminal Deoxynucleotidyl Transferase (TdT) is an intracellular marker normally present at high levels in cortical thymocytes
and a minor subset of bone marrow pre-lymphocytes.
TdT Monoclonal Kit
IM0558 RUO 20 tests
Normal Goat Serum 1 vial
anti-TdT monoclonal antibody 1 vial
FITC secondary antibody 1 vial
Mounting medium 1 vial
TdT Polyclonal Kit
IM0404 RUO 20 tests
Normal Goat Serum 1 vial
anti-TdT polyclonal antibody 1 vial
FITC secondary antibody 1 vial
Mounting medium 1 vial
53
Systems and Kits
IOTest Beta Mark TCR V Repertoire Kit
Status Part # Size
The IOTest Beta Mark Kit is a multi parametric analysis tool designed for quantitative determination of the TCR V repertoire
of human T lymphocytes by flow cytometry. Taking advantage of the fact that V specificities may be grouped into mutually
exclusive combinations, the detection of 3 V expressions in the same tube is possible with the use of an innovative staining
strategy combining three monoclonal antibodies (mAb) with only two fluorophores. A first mAb is FITC-conjugated, a second
one is PE-conjugated and a third one is a carefully balanced mixture of a PE- and a FITC-conjugated form. This kit simplifies
the TCR V repertoire analysis by reducing the number of tubes to analyze (8 instead of 24) and consequently the time
required for obtaining the results. Moreover, this kit makes it possible to study the repertoire on T cell subsets, using
additional T-cell markers, such as CD3, CD4, and CD8, conjugated to a third fluorophore. The kit is composed of 8 vials
containing mixtures of conjugated TCR V antibodies corresponding to 24 different specificities (about 70% coverage of
normal human TCR V repertoire). The test is intended for use on whole blood samples. The staining protocol includes a one-
step procedure with directly conjugated antibody mixes (test volume of 20 L) and a wash step after staining. A third color
T-cell marker (preferentially as PC5-conjugate; not included) is used to gate the specific population. The kit can be used in a
multi parametric environment employing at least three-color flow cytometric analysis. The test is compatible with different
methods of red blood cell lysis and can be run on Beckman Coulter as well as BD Biosciences cytometers. The IOTest Beta
Mark kit includes a control for PMT setting and fluorescence compensation adjustments. Since the reagent in vial A is used as
to prepare this control, the kit contains 50 tests of reagent A instead of 25.
IOTest Mark TCR V Repertoire Kit IM3497 RUO 25 tests
V5.3 Vial A 3D11 (IgG1 Mouse) PE
V7.1 ZOE (IgG2a Mouse) PE+FITC
V3 CH92 (IgM Mouse) FITC
V9 Vial B FIN9 (IgG2a Mouse) PE
V17 E17.5F3.15.13 (IgG1
Mouse)
PE+FITC
V16 TAMAYA1.2 (IgG1
Mouse)
FITC
V18 Vial C BA62.6 (IgG1 Mouse) PE
V5.1 IMMU 157 (IgG2a
Mouse)
PE+FITC
V20 ELL1.4 (IgG2a Mouse) FITC
V13.1 Vial D IMMU 222 (IgG2b
Mouse)
PE
V13.6 JU74.3 (IgG1 Mouse) PE+FITC
V8 56C5.2 (IgG2a Mouse) FITC
V5.2 Vial E 36213 (IgG1 Mouse) PE
V2 MPB2D5 (IgG1 Mouse) PE+FITC
V12 VER2.32.1 (IgG2a
Mouse)
FITC
V23 Vial F AF23 (IgG1 Mouse) PE
V1 BL37.2 (IgG1 Rat) PE+FITC
V21.3 IG125 (IgG2a Mouse) FITC
V11 Vial G C21 (IgG2a Mouse) PE
V22 IMMU 546 (IgG1 Mouse) PE+FITC
V14 CAS1.1.3 (IgG1 Mouse) FITC
V13.2 Vial H H132 (IgG1 Mouse) PE
V4 WJF24 (IgM Rat) PE+FITC
V7.2 ZIZOU4 (IgG2a Mouse) FITC
54
Systems and Kits
Solastra
The Solastra 5-color reagent combinations are intended
for identifying lineages of normal and abnormal cells that
may be found in the blood stream (and bone marrow) of
patients with known or suspected hematolymphoid
neoplasia. They are composed against B, T, and
Myelomonocytic lineage antigens and reflect the minimum
number of antibodies recommended by the Bethesda
International Consensus conference held in 2006 (Brent
L. Wood, et al., Cytometry Part B [Clinical Cytometry],
2007, 72B:S14S22).
Status Part # Size
The reagent kit combines two five-color reagent formulations of fluorescent-labeled monoclonal antibodies, Solastra BL1 and Solastra BL2,
which are intended for use on the Cytomics FC 500 flow cytometry system. The kit is intended for the identification and enumeration of
CD19+ Kappa+, CD19+Lambda+, CD5+, CD19+, CD19+CD38+, CD19+CD10+, CD20+, CD10+, and CD38+ percentages on leukocytes.
Solastra B Lineage Kit
A66286 CE 2 vials
Kappa-FITC/Lambda-PE/CD19-ECD/CD5-PC5.5/CD45-PC7 25 tests 1 vial Solastra BL1
CD20-FITC/CD10-PE/CD19-ECD/CD38-PC5.5/CD45-PC7 25 tests 1 vial Solastra BL2
The reagent kit combines two reagent formulations (one four-color and one five-color) of fluorescent-labeled monoclonal antibodies, Solastra
TL1 and Solastra TL2, which are intended for use on the Cytomics FC 500 flow cytometry system. The kit is intended for the identification and
enumeration of CD2+, CD3+, CD3+CD4+, CD5+, CD7+, CD3+CD8+ and CD56+ percentages on leukocytes.
Solastra T Lineage Kit
A66287 CE 2 vials
CD2-FITC/CD56-PE/CD7-ECD/CD5-PC5.5/CD45-PC7 25 tests 1 vial Solastra TL1
CD8-FITC/CD4-PE/CD3-PC5.5/CD45-PC7 25 tests 1 vial Solastra TL2
The reagent kit combines three five-color reagent formulations of fluorescent-labeled monoclonal antibodies, Solastra ML1, Solastra ML2, and
Solastra ML3, which are intended for use on the Cytomics FC 500 flow cytometry system. The kit is intended for the identification and
enumeration of CD15+, CD11b+, CD16+, CD14+, CD34+, CD56+, HLA-DR+, CD117+, CD13+, CD7+ and CD33+ percentages on leukocytes.
Solastra Myelomonocytic Lineage Kit
A66288 CE 3 vials
CD15-FITC/CD11b-PE/CD16-ECD/CD14-PC5.5/CD45-PC7 25 tests 1 vial Solastra ML1
HLA-DR-FITC/CD56-PE/CD34-ECD/CD117-PC5.5/CD45-PC7 25 tests 1 vial Solastra ML2
CD7-FITC/CD13-PE/CD34-ECD/CD33-PC5.5/CD45-PC7 25 tests 1 vial Solastra ML3
55
Systems and Kits
Trillium Dx FETALtrol
It is an established laboratory practice to use stabilized
control cells to monitor the performance of diagnostic
tests. FETALtrol is a tri-level, assayed, stabilized human
blood control designed to document and monitor values
obtained from test methods used to determine fetal red
blood cells (RBCs) in maternal blood samples. The fetal
RBCs in the product are Rho or D antigen positive and the
adult RBCs are Rho or D antigen negative.
The laboratory determination of the level of fetal cells in
maternal circulation remains an important support in the
obstetrical management of women with suspected uterine
trauma and in the proper dose administration of Rh
immune globulin. FETALtrol provides a means of
monitoring preparation techniques, stains, reagents, and
methods of data analysis for quantitative and qualitative
tests.
Status Part # Size
Fetal Red Blood Cell Controls
Features:
FETALtrol is a stabilized blood product that handles like patient blood samples
It eliminates the need to acquire cord blood and prepare home brew controls
Assayed values at three levels serve as both a quality measure for the assay method, as well as a reference material for
proper gating.
FETALtrol is a trademark or registered trademark of Trillium Diagnostics, LLC in the Unites States and in several other jurisdictions.
FETALtrol Kit
B03279 IVD & CE 100 tests
Level 1 (N) - Normal or Negative 2 mL / vial 2 vials Green cap and label
Level 2 (L) - Low Positive 2 mL / vial 2 vials Blue cap and label
Level 3 (H) - High Positive 2 mL / vial 2 vials Red cap and label
Not available in all geographies.
56
Systems and Kits
Trillium Dx Leuko64
Neutrophil CD64 expression is increased in case of
inflammation.
The Leuko64 assay uses a mixture of three monoclonal
antibodies with specificities to CD64 (clones 22 and 32.2)
and CD163 (clone Mac2-158), a red blood cell (RBC)
lysing solution, and a fluorescent bead suspension for
instrument calibration and standardization of the
leukocyte CD64 and CD163 expression in human blood.
Features:
Requires only 50 L of blood and under one hour of
hands-on time
Superior evaluation of neutrophil activation
during inflammation
Standardized assay with internal calibrator and controls
User-friendly software allows for excellent
reproducibility
Status Part # Size
Assay for Detection of Neutrophil Inflammation
Leuko64 Kit (75 tests)
B03284 CE 75 tests For Flow Cytometry
Mixture of murine monoclonal antibodies
Lyse solution 10X concentrated
Polystyrene beads labeled with a red dye and FITC 5.2 m
Not available in all geographies
Leuko64 Kit (250 tests)
B03285 CE 250 tests For Flow Cytometry
Mixture of murine monoclonal antibodies
Lyse solution 10X concentrated
Polystyrene beads labeled with a red dye and FITC 5.2 m
Not available in all geographies
57
Instrument Accessories
EPICS XL Accessories
Status Part #
-
6912932
EPICS XL 4th Color Upgrade Kit
-
6913337
EPICS XL PSC Quick Scan Bar Code Gun
-
6915493
-
Please call
EPICS XL-MCL Upgrade Kit
Quanta Series Accessories
Status Part #
RUO
629967
Iso-Diluent, 10 L
RUO
629966
Iso-Diluent, 20 L
RUO
629968
Shutdown Solution, 5 L
RUO
629969
Cleaning Solution Kit, 4 x 50 mL
RUO
629972
DNA Reference Calibrator, 2 x 5 mL
RUO
731085
Nuclear Isolation and Stain (NIM-DAPI 10), 250 mL
RUO
731086
Nuclear Isolation Media (NIM), 50 mL
RUO
731087
30 m Filter tip, 100 tips
RUO
731088
Marine Iso-Diluent, 10 L
IVD & CE
6605359
Softwares
Status Part #
-
A38415
21 CFR Part 11 Software option
-
6915048
-
Please call
-
Please call
-
6415063
MultiCycle for Windows*
58
Instrument Support Reagents
COULTER CLENZ Cleaning Agent is an all-purpose cleaning reagent. It contains agents that strip away protein
build-up and lysed red blood cells in instrument fluidics. It should be used routinely as part of regular
instrument maintenance, such as shutdown. Additionally, it should be included in a schedule of routine
preventive maintenance procedures designed to maintain high performance.
COULTER CLENZ
Format Size Status Part #
Cleaning Agent
IVD 8546929 Liquid 1 x 500 mL
IVD 8546930 Liquid 1 x 5 L
IVD 8546931 Liquid 1 x 10 L
This isotonic fluid is specifically formulated for use in flow cytometers. IsoFlow Sheath Fluid is carefully
manufactured for low particulate and fluorescence backgrounds to ensure superior signal to noise ratio
measurements.
IsoFlow Sheath Fluid
Sheath Fluid
IVD & CE 8547008 Liquid 4 x 1.8 L
IVD & CE 8546859 Liquid 1 x 10 L
ISOTON II
Diluent
LUO 8546719 Liquid 1 x 20 L
PuraFlow Sheath Fluid
Sheath Fluid
RUO A70188 Liquid, 1X 20 L
RUO CY30230 Liquid, 8X 4 x 6 L
59
Instrument Support Reagents
Rinsol Cleaning Agent is used to quickly and effectively clean the sample lines on the CellPrep system, ensuring
efficient and trouble-free operation.
Rinsol Cleaning Agent
Cleaning agent
IVD & CE 8547198 Liquid 1 x 5 L
Sample Tubes
For FC 500 and EPICS XL Flow Cytometers
- 2523749 - 250 tubes
60
Multi-Color Reagents
Single-Color Reagents
- Table of Multi-Colors ---------------------------- 62 - 70
- Multi-Color Reagents to Human Antigens ----- 71 - 94
- Table of Single-Colors -------------------------- 95 - 98
- Antibodies to Human CDs --------------------- 99 - 193
- Antibodies to Human non-CD Antigens ----- 194 - 244
61
P E
or RD1
E C D P C 5 P C 7 R U O A S R C E I V D
P a r t N u m b e r
F I T C
Table of Multi-Color Reagents
CD2
2-color
IM1289U CD2 CD19
6604239 CD2 CD7
6603801 CD19 CD2 6603801
CD3
4-color
6607013 CD45 CD4 CD8 CD3 6607013
6607073 CD45 CD56 CD19 CD3 6607073
3-color
CD3 CD4 CD45 IM1651
CD3 CD8 CD45 IM1670
CD3 CD19 CD45 IM1671
CD4 CD8 CD3 IM1650
IM3484U CD5 CD7 CD3
IM3485U CD8 CD4 CD3
6607053 CD8 CD4 CD3 6607053
CD8 CD4 CD3 6607103
IM3487U CD16 CD56 CD3
6607015 CD45 CD4 CD3 6607015
6607017 CD45 CD8 CD3 6607017
6607071 CD45 CD56 CD3 6607071
6607072 CD45 CD19 CD3 6607072
IM3464U MPO CD79a CD3
Neg.Ctrl. Neg.Ctrl. CD3
2-color
IM2075U CD3 CD56
IM1287U CD3 CD4
IM1310U CD3 CD8
CD3 CD(16+56) IM2076
IM1293U CD3 CD19
IM2053U CD3 HLA-DR
IM1291U CD3 CD16
CD3 CD4 IM1382
CD3 CD8 IM1383
CD3 CD19 IM1384
CD3 HLA-DR IM1390
IM2667U CD3 CD25
6604929 CD3 CD4 6604929
6604930 CD3 CD8 6604930
6605015 CD3 CD19 6605015
6607068 CD3 CD56 6607068
CD3 CD56 A07415
IM1295U CD3 HLA-DR
HLA-DR CD3 IM1300
62
P E
or RD1
P a r t N u m b e r
F I T C
CD4
4-color
6607013 CD45 CD4 CD8 CD3 6607013
3-color
CD3 CD4 CD45 IM1651
CD4 CD8 CD3 IM1650
IM3485U CD8 CD4 CD3
6607053 CD8 CD4 CD3 6607053
CD8 CD4 CD3 6607103
6607015 CD45 CD4 CD3 6607015
2-color
IM1287U CD3 CD4
CD3 CD4 IM1382
6604929 CD3 CD4 6604929
IM0747U CD4 CD8
CD4 CD8 IM1385
6603799 CD4 CD29
6603800 CD4 CD45RA
6604614 CD4 CD8 6604614
IM1379U CD8 CD4
6603802 CD8 CD4 6603802
CD45RA CD4 IM2762
CD5
3-color
IM3484U CD5 CD7 CD3
2-color
IM1346U CD19 CD5
CD7
3-color
IM3484U CD5 CD7 CD3
IM3483U HLA-DR CD7 CD45
2-color
6604239 CD2 CD7
CD8
4-color
6607013 CD45 CD4 CD8 CD3 6607013
3-color
CD3 CD8 CD45 IM1670
CD4 CD8 CD3 IM1650
IM3485U CD8 CD4 CD3
6607053 CD8 CD4 CD3 6607053
CD8 CD4 CD3 6607103
6607017 CD45 CD8 CD3 6607017
63
P E
or RD1
P a r t N u m b e r
F I T C
2-color
IM1310U CD3 CD8
CD3 CD8 IM1383
6604930 CD3 CD8 6604930
IM0747U CD4 CD8
CD4 CD8 IM1385
6604614 CD4 CD8 6604614
IM1379U CD8 CD4
IM2695U CD8 CD38
6603798 CD8 CD56
6603802 CD8 CD4 6603802
IM2673U CD38 CD8
CD57 CD8 IM1198
IM1199U HLA-DR CD8
CD10
3-color
2-color
6604241 CD10 CD19
CD11c
3-color
IM3470U CD25 CD11c CD19
CD13
3-color
CD14
3-color
2-color
IM1201U CD45 CD14
CD45 CD14 IM1387
6603909 CD45 CD14 6603909
CD(14+16)
3-color
CD(14+16) CD85k CD33 A07405
CD(14+16) CD85k CD123 A07412
CD15
3-color
64
P E
or RD1
P a r t N u m b e r
F I T C
CD16
3-color
2-color
IM1291U CD3 CD16
CD(16+56)
2-color
CD3 CD(16+56) IM2076
CD19
4-color
6607073 CD45 CD56 CD19 CD3 6607073
3-color
6607072 CD45 CD19 CD3 6607072
IM3468U FMC7 CD23 CD19
IM3465U Kappa Lambda CD19
2-color
IM1289U CD2 CD19
IM1293U CD3 CD19
CD3 CD19 IM1384
6605015 CD3 CD19 6605015
6604241 CD10 CD19
IM1346U CD19 CD5
6603801 CD19 CD2 6603801
CD20
3-color
CD23
3-color
CD25
3-color
2-color
IM2667U CD3 CD25
65
P E
or RD1
P a r t N u m b e r
F I T C
CD29
2-color
6603799 CD4 CD29
CD33
3-color
CD(14+16) CD85k CD33 A07405
CD34
3-color
IM3477U CD34 CD117 CD45
CD36
2-color
IM2279U CD36 CD235a
CD38
3-color
2-color
IM2695U CD8 CD38
IM2673U CD38 CD8
CD41
3-color
IM3482U CD41 CD235a CD45
CD45
4-color
6607013 CD45 CD4 CD8 CD3 6607013
6607073 CD45 CD56 CD19 CD3 6607073
3-color
CD3 CD4 CD45 IM1651
CD3 CD8 CD45 IM1670
IM3477U CD34 CD117 CD45
6607015 CD45 CD4 CD3 6607015
6607017 CD45 CD8 CD3 6607017
6607019 CD45 IgG1 IgG1 6607019
6607071 CD45 CD56 CD3 6607071
6607072 CD45 CD19 CD3 6607072
66
P E
or RD1
P a r t N u m b e r
F I T C
IM3489U Neg.Ctrl. Neg.Ctrl. CD45
IgG1 IgG1 CD45 IM1673
2-color
IM1201U CD45 CD14
CD45 CD14 IM1387
IM2280U CD45 CD235a
6603909 CD45 CD14 6603909
CD45RA
2-color
6603800 CD4 CD45RA
CD45RA CD4 IM2762
CD56
4-color
6607073 CD45 CD56 CD19 CD3 6607073
3-color
6607071 CD45 CD56 CD3 6607071
2-color
IM2075U CD3 CD56
6607068 CD3 CD56 6607068
CD3 CD56 A07415
6603798 CD8 CD56
CD57
2-color
CD57 CD8 IM1198
CD71
3-color
CD79a
3-color
CD85k
3-color
CD(14+16) CD85k CD33 A07405
CD(14+16) CD85k CD123 A07412
CD103
3-color
CD117
3-color
IM3477U CD34 CD117 CD45
67
P E
or RD1
P a r t N u m b e r
F I T C
CD123
3-color
CD(14+16) CD85k CD123 A07412
CD235a
3-color
2-color
IM2279U CD36 CD235a
IM2280U CD45 CD235a
FMC7
3-color
HLA-B7
2-color
HLA-B27 HLA-B7 IM1502
HLA-B27
2-color
HLA-B27 HLA-B7 IM1502
HLA-DR
3-color
2-color
IM2053U CD3 HLA-DR
CD3 HLA-DR IM1390
IM1295U CD3 HLA-DR
IM1199U HLA-DR CD8
HLA-DR CD3 IM1300
Kappa
3-color
Lactoferrin
2-color
MPO Lactoferrin IM2743
Lambda
3-color
MPO
3-color
2-color
MPO Lactoferrin IM2743
68
P E
or RD1
P a r t N u m b e r
F I T C
Neg.Ctrl.
3-color
IM3488U Neg.Ctrl. Neg.Ctrl. Neg.Ctrl.
IgG1
3-color
6607019 CD45 IgG1 IgG1 6607019
6607019 CD45 IgG1 IgG1 6607019
IgG1 IgG1 IgG1 IM1672
6607054 IgG1 IgG1 IgG1 6607054
6607054 IgG1 IgG1 IgG1 6607054
6607054 IgG1 IgG1 IgG1 6607054
2-color
IM1203U IgG1 IgG1
IM1203U IgG1 IgG1
A10974 IgG1 IgG2a
IgG1 IgG1 IM1388
IgG1 IgG1 IM1388
IgG1 IgG2a A17599
6603796 IgG1 IgG1 6603796
6603796 IgG1 IgG1 6603796
6603914 IgG1 IgM
IgG1 IgG2b 6604238
6603795 IgG2a IgG1 6603795
IgG2a IgG1 B09981
IgG2a
2-color
A10974 IgG1 IgG2a
IgG1 IgG2a A17599
IgG2a IgG2a A12695
IgG2a IgG2a A12695
6603795 IgG2a IgG1 6603795
IgG2a IgG2b 6604240
IgG2a IgG1 B09981
69
P E
or RD1
P a r t N u m b e r
F I T C
IgG2b
2-color
IgG1 IgG2b 6604238
IgG2a IgG2b 6604240
IgM
2-color
6603914 IgG1 IgM
70
CYTO-STAT
4 color
CD45-FITC B3821F4A IgG2b (mouse)
CD4-RD1 SFCI12T4D11 IgG1 (mouse)
CD8-ECD SFCI21Thy2D3 IgG1 (mouse)
CD3-PC5 UCHT1 IgG1 (mouse)
Clone Isotype Combination
Size Status Part # Line
6607013 IVD & CE 50 tests CYTO-STAT
CD56-RD1 N901 IgG1 (mouse)
CD19-ECD J3-119 IgG1 (mouse)
3 color
CD8-FITC/CD4-PE/CD3-PC5
CD8-FITC SFCI21Thy2D3 IgG1 (mouse)
71
CD8-RD1 SFCI21Thy2D3 IgG1 (mouse)
CD19-RD1 J3-119 IgG1 (mouse)
CD45-FITC/IgG1-PE/IgG1-PC5
IgG1-RD1 2T8-2F5 IgG1 (mouse)
IgG1-PC5 2T8-2F5 IgG1 (mouse)
72
IgG1-FITC/IgG1-PE/IgG1-PC5
IgG1-FITC 2T8-2F5 IgG1 (mouse)
IgG1-PC5 2T8-2F5 IgG1 (mouse)
2 color
CD2-FITC/CD7-PE
CD2-FITC SFCI3Pt2H9 IgG1 (mouse)
CD7-RD1 3A1E-12H7 IgG2b (mouse)
6604239 ASR 0.5 mL CYTO-STAT
CD3-FITC/CD4-PE
CD3-FITC UCHT1 IgG1 (mouse)
CD3-FITC/CD8-PE
CD3-FITC/CD19-PE
CD19-RD1 89B IgG1 (mouse)
73
CD3-FITC/CD56-PE
CD4-FITC/CD8-PE
CD4-FITC SFCI12T4D11 IgG1 (mouse)
CD4-FITC/CD29-PE
CD29-RD1 4B4LDC9LDH8 IgG1 (mouse)
CD4-FITC/CD45RA-PE
CD45RA-RD1 2H4LDH11LDB9 IgG1 (mouse)
CD8-FITC/CD4-PE
74
CD8-FITC/CD56-PE
CD10-FITC/CD19-PE
CD10-FITC J5 IgG2a (mouse)
CD19-RD1 HD237 IgG2b (mouse)
CD19-FITC/CD2-PE
CD19-FITC 89B IgG1 (mouse)
CD2-RD1 SFCI3Pt2H9 IgG1 (mouse)
CD45-FITC/CD14-PE
CD45-FITC KC56 IgG1 (mouse)
CD14-RD1 116 IgM (mouse)
IgG1-FITC/IgG1-PE
75
IgG1-FITC/IgG2b-PE
IgG2b-RD1 MPC-11 IgG2b (mouse)
6604238 RUO 50 tests CYTO-STAT
IgG1-FITC/IgM-PE
IgM-RD1 R4A3-22-12 IgM (mouse)
IgG2a-FITC/IgG1-PE
IgG2a-FITC 7T4-1F5 IgG2a (mouse)
IgG2a-FITC/IgG2b-PE
IgG2b-RD1 MPC-11 IgG2b (mouse)
6604240 RUO 50 tests CYTO-STAT
76
IOTest
3 color
CD4-PE SFCI12T4D11 IgG1 (mouse)
6607103 RUO 50 tests IOTest
CD(14+16)-FITC/CD85k-PE/CD33-PC5
CD(14+16)-FITC RMO52+3G8 IgG2a+IgG1 (mouse)
CD85k-PE ZM3.8 IgG1 (mouse)
CD33-PC5 D3HL60.251 IgG1 (mouse)
A23413 CE 50 tests IOTest
A07405 RUO 50 tests IOTest
CD(14+16)-FITC/CD85k-PE/CD123-PC5
CD(14+16)-FITC RMO52+3G8 IgG2a+IgG1 (mouse)
CD85k-PE ZM3.8 IgG1 (mouse)
CD123-PC5 SSDCLY107D2 IgG1 (mouse)
2 color
CD2-FITC/CD19-PE
CD2-FITC 39C1.5 IgG2a (rat)
CD19-PE J3-119 IgG1 (mouse)
IM1289U ASR 1 mL IOTest
77
CD3-FITC/CD4-PE
CD4-PE 13B8.2 IgG1 (mouse)
CD3-FITC/CD8-PE
CD8-PE B9.11 IgG1 (mouse)
CD3-FITC/CD16-PE
CD16-PE 3G8 IgG1 (mouse)
CD3-FITC/CD(16+56)-PE
CD(16+56)-PE 3G8+N901 IgG1 (mouse)
IM2076 RUO 50 tests IOTest
CD3-FITC/CD19-PE
78
CD3-FITC/CD25-PE
CD25-PE B1.49.9 IgG2a (mouse)
CD3-FITC/CD56-PC5
CD56-PC5 N901 IgG1 (mouse)
CD3-FITC/CD56-PE
CD56-PE N901 IgG1 (mouse)
CD3-FITC/HLA-DR-PE
HLA-DR-PE B8.12.2 IgG2b (mouse)
CD3-FITC/HLA-DR-PE
HLA-DR-PE Immu-357 IgG1 (mouse)
79
CD4-FITC/CD8-PE
CD4-FITC 13B8.2 IgG1 (mouse)
CD8-FITC/CD4-PE
CD8-FITC B9.11 IgG1 (mouse)
CD8-FITC/CD38-PE
CD38-PE LS198-4-3 IgG1 (mouse)
CD19-FITC/CD5-PE
CD19-FITC J3-119 IgG1 (mouse)
CD5-PE BL1a IgG2a (mouse)
CD36-FITC/CD235a-PE
CD36-FITC FA6.152 IgG1 (mouse)
CD235a-PE 11E4B-7-6 IgG1 (mouse)
80
CD38-FITC/CD8-PE
CD38-FITC T16 IgG1 (mouse)
CD45-FITC/CD14-PE
CD45-FITC Immu19.2 IgG1 (mouse)
CD14-PE RMO52 IgG2a (mouse)
CD45-FITC/CD235a-PE
CD45-FITC J.33 IgG1 (mouse)
CD45RA-FITC/CD4-PE
CD45RA-FITC ALB11 IgG1 (mouse)
CD57-FITC/CD8-PE
CD57-FITC NC1 IgM (mouse)
81
HLA-B27-FITC/HLA-B7-PE
HLA-B27-FITC HLA-ABC-m3 IgG2a (mouse)
HLA-B7-PE BB7.1 IgG1 (mouse)
HLA-DR-FITC/CD3-PE
HLA-DR-FITC B8.12.2 IgG2b (mouse)
CD3-PE UCHT1 IgG1 (mouse)
HLA-DR-FITC/CD8-PE
HLA-DR-FITC B8.12.2 IgG2b (mouse)
IgG1-FITC/IgG1-PE
IgG1-FITC 679.1Mc7 IgG1 (mouse)
IgG1-PE 679.1Mc7 IgG1 (mouse)
IgG1-FITC/IgG2a-PE
IgG2a-PE 7T4-1F5 IgG2a (mouse)
A10974 ASR 1 mL IOTest
82
IgG2a-FITC/IgG1-PE
B09981 RUO 50 tests IOTest
IgG2a-FITC/IgG2a-PE
MPO-FITC/Lactoferrin-PE
MPO-FITC CLB-MPO-1 IgG2a (mouse)
Lactoferrin-PE CLB13.17 IgG1 (mouse)
83
IOTest 3
3 color
CD5-FITC/CD7-PE/CD3-ECD
CD5-FITC BL1a IgG2a (mouse)
CD7-PE 8H8.1 IgG2a (mouse)
CD3-ECD UCHT1 IgG1 (mouse)
IM3484U ASR 0.5 mL IOTest 3
A07725 CE 25 tests IOTest 3
CD10-PE ALB1 IgG1 (mouse)
CD23-PE 9P25 IgG1 (mouse)
84
CD14-FITC RMO52 IgG2a (mouse)
CD13-PE SJ1D1 IgG1 (mouse)
CD45-ECD J.33 IgG1 (mouse)
CD14-FITC RMO52 IgG2a (mouse)
CD16-PE 3G8 IgG1 (mouse)
CD15-FITC 80H5 IgM (mouse)
CD33-PE D3HL60.251 IgG1 (mouse)
CD16-FITC 3G8 IgG1 (mouse)
85
CD19-FITC J3-119 IgG1 (mouse)
CD20-FITC B9E9 IgG2a (mouse)
CD25-FITC B1.49.9 IgG2a (mouse)
CD25-FITC/CD11c-PE/CD19-ECD
CD25-FITC B1.49.9 IgG2a (mouse)
CD11c-PE BU15 IgG1 (mouse)
86
CD34-FITC 581 IgG1 (mouse)
CD34-FITC 581 IgG1 (mouse)
CD117-PE 104D2D1 IgG1 (mouse)
CD38-FITC T16 IgG1 (mouse)
CD41-FITC/CD235a-PE/CD45-ECD
CD41-FITC P2 IgG1 (mouse)
87
CD71-FITC YDJ1.2.2 IgG1 (mouse)
CD103-FITC 2G5 IgG2a (mouse)
CD11c-PE BU15 IgG1 (mouse)
FMC7-FITC/CD23-PE/CD19-ECD
FMC7-FITC FMC7 IgM (mouse)
CD23-PE 9P25 IgG1 (mouse)
HLA-DR-FITC/CD13-PE/CD45-ECD
HLA-DR-FITC Immu-357 IgG1 (mouse)
CD13-PE SJ1D1 IgG1 (mouse)
88
CD34-PE 581 IgG1 (mouse)
CD7-PE 8H8.1 IgG2a (mouse)
Kappa-FITC/Lambda-PE/CD19-ECD
Kappa-FITC Polyclonal-kappa IgG (goat)
Lambda-PE Polyclonal-lambda IgG (goat)
cMPO-FITC/cCD79a-PE/cCD3-ECD
MPO-FITC CLB-MPO-1 IgG2a (mouse)
CD79a-PE HM47 IgG1 (mouse)
89
Neg.Ctrl.-FITC/Neg.Ctrl.-PE/CD3-ECD
Neg.Ctrl.-FITC 679.1Mc7 IgG1 (mouse)
Neg.Ctrl.-PE 679.1Mc7 IgG1 (mouse)
Neg.Ctrl.-FITC/Neg.Ctrl.-PE/Neg.Ctrl.-ECD
Neg.Ctrl.-ECD 679.1Mc7 IgG1 (mouse)
90
OptiClone
3 color
CD45-PC5 Immu19.2 IgG1 (mouse)
IM1651 RUO 50 tests OptiClone
91
IgG1-FITC/IgG1-PE/CD45-PC5
IgG1-FITC/IgG1-PE/IgG1-PC5
IgG1-PC5 679.1Mc7 IgG1 (mouse)
2 color
CD3-FITC/CD4-PE
CD3-FITC/CD8-PE
92
CD3-FITC/CD19-PE
CD3-FITC/HLA-DR-PE
HLA-DR-PE Immu-357 IgG1 (mouse)
CD4-FITC/CD8-PE
CD45-FITC/CD14-PE
CD45-FITC Immu19.2 IgG1 (mouse)
CD14-PE RMO52 IgG2a (mouse)
IgG1-FITC/IgG1-PE
93
IgG1-FITC/IgG2a-PE
A17599 RUO 50 tests OptiClone
94
P E E C D P C 5 APC F I T C PC7
APC
A700
APC
A750
PB P C 5.5
Krome
Orange
Table of Single-Color Reagents
CD1a

CD2

CD3

CD4

CD5

CD7

CD8

CD8

CD9

CD10

CD11a

CD11b

CD11c

CD13

CD14

CD15

CD16

CD16b

CD18

CD19

CD20

CD21

CD22

CD23

CD24

CD25

CD26

CD27

CD28

CD29

CD30

CD31

CD32

CD33

CD34

CD35

CD36

CD37

CD38

CD40

CD41

CD42a

95
APC
A700
APC
A750
PB P C 5.5
Krome
Orange
CD42b

CD43

CD44

CD45

CD45R0

CD45RA

CD48

CD49b

CD49d

CD49e

CD50

CD51

CD54

CD55

CD56

CD57

CD58

CD59

CD61

CD62L

CD62P

CD63

CD64

CD65

CD66b

CD69

CD71

CD79a

CD79b

CD80

CD81

CD83

CD85a

CD85d

CD85j

CD85k

CD86

CD89

CD90

CD94

CD95

CD103

96
APC
A700
APC
A750
PB P C 5.5
Krome
Orange
CD105

CD106

CD109

CD111

CD112

CD116

CD117

CD118

CD120a

CD120b

CD122

CD123

CD124

CD126

CD127

CD130

CD134

CD135

CD136

CD138

CD144

CD146

CD152

CD154

CD157

CD158a,h

CD158b1/b2,j

CD158e1/e2

CD158i

CD159a

CD160

CD161

CD166

CD179a

CD184

CD203c

CD206

CD207

CD208

CD209

CD226

CD235a

97
APC
A700
APC
A750
PB P C 5.5
Krome
Orange
CD243

CD244

CD272

CD273

CD274

CD278

CD279

CD294

CD300a

CD309

CD314

CD335

CD336

CD337

98
Human CDs
The CD1a antigen is a membrane glycoprotein with a molecular weight of 49 kDa
expressed in association with 2-microglobulin. This antigen is found on cortical
thymocytes (strongly), Langerhans cells and interdigitating cells. The CD1a antigen is also
present on a B cell subset. CD1a antibodies are useful in the definition of the thymic stage
of T cells.
CD1a
Histiocytosis X (AEC)
Clone: SFCI19Thy1A8 (T6) Isotype: IgG1 Mouse
T6 (ref 6T-CD1.7) was used as a CD1a reference monoclonal antibody during HLDA 6. Staining pattern: membrane.
1
C I F
Part# Status
Size
Form Format Line
COULTER CLONE Liquid RUO 6603185 100 tests RD1
Clone: BL6 Isotype: IgG1 Mouse
The BL6 antibody recognizes the C epitope of the CD1a molecule. Staining pattern: membrane.
3
C F I
Part# Status
Size
Form Format Line
- Freeze-dried RUO IM0130 0.2 mg Purified
IOTest Liquid CE A07742 100 tests PE
IOTest Liquid ASR IM1942U 2 mL PE
IOTest Liquid ASR IM3610 1 mL PC5
IOTest Liquid ASR IM3645 1 mL APC
Clone: O10 Isotype: IgG1 Mouse
Tissue reactivity: O10 reacts with cortical thymocytes, interdigitating reticulum cells, and Langerhans cells. Staining pattern:
membrane.
4
C I F P
Part# Status
Size
Form Format Line
IOPath Liquid RUO IM1590 6 mL Purified
The CD1c antigen is a membrane glycoprotein with a molecular weight of 43 kDa expressed in association with 2-
microglobulin. It is found on cortical thymocytes (weakly), Langerhans cells, dendritic cells and a subset of B cells, early during
ontogeny.
CD1c
Clone: L161 Isotype: IgG1 Mouse
3
C I F
Part# Status
Size
Form Format Line
APPROPRIATE USES APPROPRIATE USES APPROPRIATE USES INAPPROPRIATE USES
A Activating E Elisa P Paraffin-embedded Tissue Sections A Non-activating
B Blocking F Frozen Tissue Sections Ph Heat Treated Paraffin-embedded Tissue B Non-blocking
C Flow Cytometry I Immunoprecipitation W Western Blot I Inappropriate for Immunoprecipitation
Ci Intracytoplasmic Staining W Inappropriate for Western Blot
The blue number references the International Workshops on Human Leucocyte Differentiation Antigens (HLDA) at which an antibody was clustered.
99
Human CDs
The CD2 antigen (LFA-2) is a monomeric 50 kDa glycoprotein. It was formerly described
as the sheep red blood cell receptor, causing T-cell rosetting, and has been identified as
the ligand for CD58 (LFA-3). It is also a receptor for CD48, CD59 and CD15, which binds
to the multimeric form of CD2. CD2 is present on the majority of normal human peripheral
blood T lymphocytes and a high percentage of NK cells. It is also expressed by all
thymocytes.
CD2
Lysed normal whole blood sample gated on
lymphocytes.
Clone: SFCI3Pt2H9 (T11) Isotype: IgG1 Mouse
SFCI3Pt2H9 inhibits sheep erythrocyte rosette formation. It has been used as a CD2 reference monoclonal antibody during HLDA 6
(ref.6T-CD2.6). Staining pattern: membrane.
1
C B F
Part# Status
Size
Form Format Line
COULTER CLONE Freeze-dried IVD 6602137 100 tests Purified
COULTER CLONE Freeze-dried IVD & CE 6602389 100 tests FITC
CYTO-STAT Liquid IVD & CE 6603863 50 tests FITC
COULTER CLONE Liquid IVD & CE 6602868 100 tests RD1
CYTO-STAT Liquid IVD & CE 6603849 50 tests RD1
Clone: 39C1.5 Isotype: IgG2a Rat
39C1.5 inhibits sheep erythrocyte rosette formation. It has been used as a CD2 reference monoclonal antibody during HLDA 6 (ref 6T-
CD2.7). Staining pattern: membrane.
2
A B C
Part# Status
Size
Form Format Line
IOTest Liquid CE A07743 100 tests FITC
IOTest Liquid ASR IM0442U 2 mL FITC
IOTest Liquid CE A07745 100 tests PC5
IOTest Liquid ASR IM2634U 1 mL PC5
IOTest Liquid ASR A51074 1 mL PC7
IOTest Liquid ASR A60794 1 mL APC
IOTest Liquid ASR B01681 0.5 mL APC-Alexa Fluor* 750
IOTest Liquid ASR B09685 0.5 mL Pacific Blue*
Clone: 6F10.3 Isotype: IgG1 Mouse
6F10.3 antibody reacts with T11.2.epitope. It has been used as a CD2 reference monoclonal antibody during HLDA 6 (ref 6T-CD2.8).
The combination of 6F10.3 and 39C1.5 monoclonal antibodies activates T cells in vitro. Tissue reactivity: 6F10.3 reacts with T
lymphocytes, thymocytes and NK cells. Staining pattern: membrane.
6
C A
Part# Status
Size
Form Format Line
100
Human CDs
CD3 see TCR in Human non-CD antigens section.
The CD3 antigen is a complex of 5 invariable polypeptidic chains, , , , and
, whose molecular weights are respectively 25-28, 21, 20, 16 and 22 kDa.
The chain occurs in the CD3 complex as a disulphide linked homodimer or
as a heterodimer with the chain. CD3 is part of a bigger complex which
includes the T Cell Receptor (TCR). This complex is expressed by mature T
cells and by thymocytes. T cell activation may be induced when a foreign
antigen is presented to the TCR through MHC complex.
CD3
lymphocytes.
Clone: UCHT1 Isotype: IgG1 Mouse
UCHT1 reacts with the -chain of the CD3 complex. It was used as a CD3 reference mAb (ref.6T-CD3.1) during HLDA 6. After
permeabilization with saponin-based permeabilization reagent IntraPrep, it may be used to detect intracytoplasmic CD3 by flow
cytometry. Staining pattern: membrane.
1
I C A F E
Part# Status
Size
Form Format Line
COULTER CLONE Freeze-dried ASR A83481 100 tests Purified
- Liquid ASR IM1280 2 mL Purified
- Freeze-dried ASR IM1304 0.2 mg Purified
- Freeze-dried RUO IM1301 0.2 mg BIOT
IOTest Liquid CE A07748 100 tests ECD
IOTest Liquid ASR IM2705U 1 mL ECD
CYTO-STAT Liquid ASR 6607010 0.5 mL PC5
IOTest Liquid ASR A66327 0.5 mL PC5.5
IOTest Liquid RUO 6607100 100 tests PC7
IOTest Liquid CE 737657 100 tests PC7
IOTest Liquid CE IM2467 100 tests APC
IOTest Liquid ASR IM2467U 1 mL APC
IOTest Liquid ASR A66329 0.5 mL APC-Alexa Fluor* 750
IOTest Liquid CE A94680 50 tests APC-Alexa Fluor* 750
IOTest Liquid ASR A93687 0.5 mL Pacific Blue*
IOTest Liquid ASR B00068 0.5 mL Krome Orange
101
Human CDs
Clone: X35 Isotype: IgG2a Mouse
X35 antibody reacts with the -chain of the CD3 complex. All monoclonal antibodies have both a mitogenic effect on T lymphocytes and do
not react with T cells lacking the TCR. Staining pattern: membrane.
3
A E F
Part# Status
Size
Form Format Line
The CD4 antigen is a single-chain transmembrane glycoprotein with a 59 kDa molecular
weight. CD4 binds to a non-polymorphic region of MHC Class II molecules and is a co-
receptor in MHC Class II restricted antigen-induced activation. CD4 is expressed on a
specific subset of peripheral blood lymphocytes, the so-called T "helper" lymphocytes. It is
present on most thymocytes where it is frequently co-expressed with CD8. CD4 is also
expressed on all monocytes, although with a lower density than on CD4
+
T lymphocytes.
CD4
+
T lymphocytes are active in inducing and helping the synthesis of immunoglobulins
by B cells. CD4 is a receptor for the Human Immunodeficiency Virus type I (HIV-1)
envelope protein gp120.
CD4
lymphocytes.
Clone: 13B8.2 Isotype: IgG1 Mouse
13B8.2 monoclonal antibody antagonizes HIV effects in vitro. 13B8.2 recognizes an epitope close to the amino-acid residues 88-89
within the CD4 molecule. 13B8.2 and BL4 recognize different epitopes. 13B8.2 and BL4 are not cross-blocking. Tissue reactivity:
13B8.2 reacts with thymocytes, helper T cells and monocytes. Staining pattern: membrane.
3
W E C B
Part# Status
Size
Form Format Line
- Freeze-dried ASR IM0704 0.2 mg BIOT
IOTest Liquid ASR A96417 0.5 mL Krome Orange
102
Human CDs
Clone: SFCI12T4D11 (T4) Isotype: IgG1 Mouse
SFCI12T4D11 monoclonal antibody antagonizes HIV effects in vitro. Staining pattern: membrane.
1
F C B
Part# Status
Size
Form Format Line
CYTO-STAT Liquid ASR 6604727 0.5 mL ECD
Clone: BL4 Isotype: IgG2a Mouse
BL4 monoclonal antibody antagonizes HIV effects in vitro. BL4 was used as a CD4 reference monoclonal antibody (ref.6T-CD4.5) during
HLDA 6. BL4 and 13B8.2 recognize different epitopes. BL4 and 13B8.2 are not cross-blocking.
3
C B
Part# Status
Size
Form Format Line
103
Human CDs
The CD5 antigen is a single-chain transmembrane glycoprotein with a molecular weight of
67 kDa. The extracellular domain consists of 3 scavenger receptor cysteine-rich (SRCR)
domains of approximately 100 amino acids each. CD5 is found on all mature T
lymphocytes and on most thymocytes. CD5 is also present on a B lymphocyte subset but
is not found on granulocytes or monocytes. CD5 is a ligand for the CD72 antigen, which is
present on B lymphocytes. The CD5
+
B lymphocyte subset (known as B-1a lymphocytes)
may arise from a non-conventional B lineage (independent of bone marrow).
CD5
lymphocytes.
Clone: SFCI24T6G12 (T1) Isotype: IgG2a Mouse
SFCI24T6G12 monoclonal antibody (ref. 6T-CD5.4) was used as a CD5 reference monoclonal antibody during HLDA 6. Staining pattern:
membrane.
1
C W I F
Part# Status
Size
Form Format Line
Clone: BL1a Isotype: IgG2a Mouse
BL1a monoclonal antibody (ref. 6T-CD5.5) was used as a CD5 reference monoclonal antibody during HLDA/6. Tissue reactivity: BL1a
reacts with mantle cells. Staining pattern: membrane.
3
C I W B
Part# Status
Size
Form Format Line
IOTest Liquid ASR A33096 1 mL ECD
104
Human CDs
The CD7 antigen is a single-chain protein with a molecular weight of 40 kDa. It is expressed on T lymphocyte precursors, prior
to thymic homing. CD7 antigen is found on thymocytes and on the majority of peripheral blood T lymphocytes. It is also
expressed on most NK cells, pre-B lymphocytes and weakly on monocytes. Mature B lymphocytes and granulocytes do not
express this antigen.
CD7
Clone: 3A1E-12H7 Isotype: IgG2b Mouse
3A1E-12H7 (ref. 6T-CD7.4) was used as a CD7 reference monoclonal antibody during HLDA 6.
1
C F I
Part# Status
Size
Form Format Line
CYTO-STAT Liquid ASR 6603824 0.5 mL FITC
COULTER CLONE Freeze-dried ASR 6603825 100 tests FITC
CYTO-STAT Liquid ASR 6603822 0.5 mL RD1
COULTER CLONE Liquid ASR 6603827 1 mL RD1
Clone: 8H8.1 Isotype: IgG2a Mouse
Tissue reactivity: 8H8.1 strictly reacts with T cells, thymocytes and NK cells. Staining pattern: membrane.
2
C F I
Part# Status
Size
Form Format Line
IOTest Liquid CE IM3613 100 tests PC5
IOTest Liquid ASR A97050 0.5 mL APC
105
Human CDs
The CD8 antigen is a disulfide-linked dimer, which exists either as a CD8 homodimer or
as a CD8/ heterodimer. CD8 is required for surface expression of CD8. The
molecular weight of each monomer of or is approximately 32-34 kDa. CD8 binds to a
non-polymorphic domain (3 domain) of MHC Class I molecules. CD8 is expressed on a
subset of human peripheral blood T lymphocytes. A subset of NK cells possess the CD8
antigen but show low to medium density of expression. CD8 homodimer is expressed by
NK cells and /
+
T cells. CD8 is also present on most thymocytes where it is frequently
co-expressed with CD4, and on a subpopulation of bone marrow cells. The CD8 molecule
acts with the T Cell Receptor (TCR) as a coreceptor for MHC class I restricted antigen
recognition. CD8 is widely used as a marker of cytotoxic T lymphocytes.
CD8
lymphocytes.
Clone: SFCI21Thy2D3 (T8) Isotype: IgG1 Mouse
SFCI21Thy2D3 monoclonal antibody recognizes the chain of the CD8 molecule. Staining pattern: membrane.
1
C F B I
Part# Status
Size
Form Format Line
CYTO-STAT Liquid CE 737659 50 tests ECD
CYTO-STAT Liquid ASR 6607011 0.5 mL PC5
IOTEST Liquid ASR A99019 0.5 mL PC5.5
IOTEST Liquid ASR A99023 0.5 mL APC
106
Human CDs
Clone: B9.11 Isotype: IgG1 Mouse
The B9.11 monoclonal antibody recognizes the chain of the CD8 molecule. It was used as a CD8 reference monoclonal antibody
during HLDA 6 (ref. 6T-CD8.5).
1
C B I
Part# Status
Size
Form Format Line
IOTest Liquid ASR B08467 0.5 mL ECD
Along with CD8, the CD8 polypeptide molecule is one of the two chains which can be
expressed within the CD8 dimer. CD8 is required for surface expression of CD8. The
and glycoproteins have similar molecular weights of 32-34 kDa each. The CD8/
heterodimer is expressed by TCR /
+
CD8
+
T cells whereas TCR /
+
CD8
+
T cells and
CD8
+
NK cells exclusively express the CD8/ homodimer.
CD8
lymphocytes.
Clone: 2ST8.5H7 Isotype: IgG2a Mouse
The 2ST8.5H7 monoclonal antibody reacts with an epitope formed by the combination of the and polypeptides.
5
C I
Part# Status
Size
Form Format Line
IOTest Liquid RUO 6607123 100 tests ECD
107
Human CDs
CD9 (p24 antigen) is a single transmembrane polypeptide of 24 kDa related to the tetraspanin (TM4) family. Like other
tetraspanins (e.g. CD63, CD81, CD82, CD37, CD53, among the 20 known members), CD9 structure is composed of 4
transmembrane domains, with intracellular N and C termini. First discovered on a lymphoblastic cell line of pre-B phenotype,
CD9 was then found on platelets and within their -granules, on monocytes, pre-B cells, eosinophils, basophils and activated
T cells. The CD9 molecule associates with other surface proteins such as the 6/4 integrin (CD49f/CD104 molecule) and
HLA-DR, suggesting a role in adhesion, signal transduction and cell motility.
CD9
Clone: ALB6 Isotype: IgG1 Mouse
The ALB6 monoclonal antibody (mAb) was first reported as specific for CD9 (p24) on platelets, capable of modulating aggregation of
platelets activated with various agonists.
3
C I W A F
Part# Status
Size
Form Format Line
The CD10 antigen is referred to as the Common Acute Lymphoblastic Leukemia Antigen (CALLA). It is a type II integral
membrane protein of 100 kDa, identified as the human membrane-associated neutral endopeptidase (EC3.4.24.11). It is
expressed on uncommitted lymphoid precursors. CD10 expression is lost as cells enter the T lineage. In the B lineage, CD10
expression is lost later in ontogeny, as cells acquire surface Ig expression. It is also expressed on activated and proliferating B
cells in the germinal center, and on neutrophils as well as on bone marrow stromal cells. It is also expressed on a number of
other cells of epithelial origin.
CD10
Clone: J5 Isotype: IgG2a Mouse
Staining pattern: membrane.
1
F C I P B
Part# Status
Size
Form Format Line
F C
Part# Status
Size
Form Format Line
108
Human CDs
Clone: ALB2 Isotype: IgG2a Mouse
Tissue reactivity: ALB2 reacts with lymphoid progenitor cells, granulocytes, germinal center B cells, kidney glomeruli, smooth muscle
cells and myoepithelial cells of adult breast. Staining pattern: membrane.
3
C F
Part# Status
Size
Form Format Line
CD11a (L integrin, LFA-1 chain) is a member of the integrin family. Like some other
leucocyte integrins (CD11b, CD11c, CD11d), CD11a is non-covalently associated with
the 2 integrin subunit CD18. CD11a is a transmembrane glycoprotein of 170 kDa, mainly
expressed by leucocytes including monocytes, macrophages, neutrophils, eosinophils,
basophils, B and T lymphocytes. Activated platelets also express CD11a and CD18. LFA
-1 (CD11a/CD18) promotes homotypic adhesion between lymphoid cells and heterotypic
adhesion of leucocytes to the vascular endothelium. LFA-1s known ligands are
Intercellular Adhesion Molecules (ICAM): ICAM-1 (CD54), ICAM-2 (CD102) and ICAM-3
(CD50).
CD11a
Lysed normal whole blood.
Clone: 25.3 Isotype: IgG1 Mouse
Tissue reactivity: 25.3 reacts with lymphocytes, monocytes and neutrophils and bone marrow T lymphocytes. Staining pattern:
membrane.
4
I C B
Part# Status
Size
Form Format Line
109
Human CDs
The CD11b antigen is also known as the integrin M subunit. It combines with the CD18
antigen (integrin 2 subunit) to build the integrin Mac-1 (CD11b/CD18, M2, CR3,
iC3bR, Mo-1). CD11b is a type I transmembrane glycoprotein of 170 or 165 kDa under
reducing or non-reducing conditions, respectively. CD11b shows 19 potential N-
glycosylation sites. Expression of the CD11b chain on the cell surface requires the
presence of the CD18 antigen (also known as 2 integrin chain). Together, these two
subunits create the CD11b/CD18 integrin, one of the four integrin heterodimers that can
be built by the association of CD18 chain with four distinctive CD11 chains.
CD11b/CD18 is highly expressed on NK Cells, neutrophils, monocytes and macrophages.
CD11b
lymphocytes.
Clone: 94 (Mo1) Isotype: IgM Mouse
1
C W I B F
Part# Status
Size
Form Format Line
Clone: Bear1 Isotype: IgG1 Mouse
Bear1 is a non blocking monoclonal antibody. Tissue reactivity: in normal skin, and tonsil, the expression of CD11b is restricted to
macrophages. Staining pattern: membrane.
6
C I B F
Part# Status
Size
Form Format Line
IOTest Liquid CE IM0530 100 tests FITC
110
Human CDs
CD11c (integrin X subunit, also known as leucocyte surface antigen p150, Axb2 and
CR4) is a member of the integrin family. Like other leucocyte integrins (CD11a, CD11b,
CD11d), CD11c is non-covalently associated with the 2 integrin subunit (CD18). CD11c
is a transmembrane glycoprotein of 145-150 kDa, mainly expressed by monocytes,
macrophages and NK cells, and to a lesser extent by granulocytes, dendritic cells and
some subsets of T and B cells.
CD11c
lymphocytes.
Clone: BU15 Isotype: IgG1 Mouse
The BU15 antibody was used as a CD11c reference mAb (ref.6) during HLDA 6. Tissue reactivity: BU15 reacts with macrophages,
monocytes, granulocytes, NK cells, dendritic cells, activated T and B lymphocytes, and microglia. Staining pattern: membrane.
3
F C I
Part# Status
Size
Form Format Line
IOTest Liquid CE IM1760 100 tests PE
IOTest Liquid ASR B01680 0.5 mL APC
111
Human CDs
The CD13 antigen is a transmembrane glycoprotein with a large extracellular region and a
small intracellular NH2-terminal tail. It has a molecular weight of 150 kDa and is expressed
on the cell surface as a non-covalently linked homodimer. It is found on most cells of
myeloid origin including neutrophils, eosinophils, basophils and monocytes from normal
peripheral blood. It is absent from B and T lymphocytes as well as from red blood cells and
platelets. This molecule is found on the surface of committed progenitor cells defined as
the Granulocyte-Monocyte Colony Forming Units (CFU-GM) in normal bone marrow.
There are up to five subpopulations of CD13 molecules possessing different levels of
glycosylation, which may explain the different binding patterns of various CD13 antibodies.
CD13
monocytes.
Clone: 366 (MY7) Isotype: IgG1 Mouse
1
C I F
Part# Status
Size
Form Format Line
COULTER CLONE Freeze-dried ASR 6602626 100 tests Purified
Clone: SJ1D1 Isotype: IgG1 Mouse
3
C I F
Part# Status
Size
Form Format Line
Clone: Immu103.44 Isotype: IgG1 Mouse
5
C
Part# Status
Size
Form Format Line
112
Human CDs
The CD14 antigen is a glycosyl-phosphatidylinositol-linked single-chain surface membrane
glycoprotein with a molecular weight of 53-55 kDa. It functions as a high affinity receptor
for the complex of LipoPolySaccharide (LPS) and the LPS-Binding Protein (LBP). It is
found on cells of myelomonocytic lineage. CD14 antigen is strongly expressed on
monocytes, macrophages, and weakly on neutrophils. It is also weakly expressed on B
lymphocytes, but is absent from T lymphocytes, NK cells, red blood cells and platelets. It is
found on Langerhans cells, follicular dendritic cells and histiocytes.
CD14
Lysed normal whole blood sample.
Clone: 116 (Mo2) Isotype: IgM Mouse
1
C I F
Part# Status
Size
Form Format Line
COULTER CLONE Freeze-dried IVD 6602507 100 tests FITC
COULTER CLONE Liquid IVD 6603266 100 tests RD1
Clone: 322A-1 (MY4) Isotype: IgG2b Mouse
1
C F W I E B
Part# Status
Size
Form Format Line
Clone: RMO52 Isotype: IgG2a Mouse
6
C
Part# Status
Size
Form Format Line
113
Human CDs
The CD15 antigen (Lewis x or Le
x
) is the lacto-N-(neo) fucopentaose III molecule. This
carbohydrate epitope is carried by both glycolipids and glycoproteins expressed on the
cell membrane. CD15 antigen is strongly expressed by neutrophils, eosinophils,
monocytes, macrophages, mast cells and normal myeloid precursor cells. It is not
expressed on normal erythrocytes, platelets or lymphocytes.
CD15
Reed-Sternberg cell in Hodgkins disease.
Clone: 80H5 Isotype: IgM Mouse
Tissue reactivity: 80H5 reacts with granulocytes. Occasionally epithelioid histiocytes may be weakly stained. After neuraminidase
treatment of paraffin sections, 80H5 reacts with Langerhans cells of the skin and interdigitating reticulum cells. Many epithelial cells
(gastric glands, squamous cells and kidney tubules) are commonly positive. Staining pattern: membrane and cytoplasm (Golgi).
5
C E A P F
Part# Status
Size
Form Format Line
IOPath+ Liquid RUO IM1921 1 mL Purified
114
Human CDs
The CD16 antigen is the low-affinity receptor for IgG (FcRIII). The CD16 antigen exists in two different forms encoded by two
different genes: FcRIIIA (or III-2) and FcRIIIB (or III-1). The genetic heterogeneity of CD16 generates alternative membrane-
anchored molecules. One is a transmembrane form (FcRIIIA, 50-65 kDa) expressed on NK cells, monocytes and
macrophages. The other is a glycosylphosphatidylinositol (GPI)-anchored form (FcRIIIB, 48 kDa) only expressed on
neutrophils. It has been shown that the CD16 antigen can be non-covalently associated within the membrane of NK cells, to a
disulfide-linked homo or heterodimer made from the 16 kDa CD3 chain and the FcRIchain.
CD16
Clone: 3G8 Isotype: IgG1 Mouse
The 3G8 monoclonal antibody is inappropriate for western blot use. 3G8 binds to both isoforms of the CD16 molecule. Tissue reactivity:
the 3G8 monoclonal antibody strongly stains neutrophils and NK cells, weakly labels Kupffer cells, and subpopulations of macrophages
in the lung, placenta and kidney. Staining pattern: membrane.
4
C F B I A E
Part# Status
Size
Form Format Line
W
The IgGFc low affinity receptor (FCRIII) is known to be expressed as an integral
membrane protein (FcRIIIA) and as a glycosyl-phosphatidyl inositol (GPI)-anchored
protein (FcRIIIB). These two isoforms, encoded by two different, although linked genes,
have extracellular amino-acidic sequences differing by six amino acids and show specific
tissue distributions. FcRIIIB protein defines the CD16b cluster.
CD16b
Clone: 1D3 Isotype: IgM Mouse
The monoclonal antibody 1D3 was shown to be non-reactive with NK cells or monocytes but to be specific to polymorphonuclear
leucocytes (PMNs). It recognizes an epitope specific to the FcRIIIB molecule and was characterized as the sole antibody belonging to
the CD16b cluster of differentiation. This antibody reacts with PMNs, irrespective of the neutrophil antigen (NA) phenotype, although it
shows lower reactivity with NA2-homozygote PMNs as compared to NA1-homozygotes or NA1/NA2 heterozygotes.
5
C I F
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115
Human CDs
CD18 is the 95 kDa integrin 2-chain which forms non-covalently-bound heterodimers
with the integrin subunits: CD11a, CD11b and CD11c (L, M and X, respectively).
CD18 is expressed by all leucocytes , activated platelets and megakaryocytic cell lines.
CD18
Clone: 7E4 Isotype: IgG1 Mouse
7E4 is a blocking monoclonal antibody in cell adhesion assays. Tissue reactivity: the 7E4 monoclonal antibody stains lymphocytes,
granulocytes and macrophages in tonsil, lymph nodes and liver. It is found in the small bowel with increased expression in inflamed
tissues. Staining pattern: membrane.
6
B I C
Part# Status
Size
Form Format Line
116
Human CDs
The CD19 antigen (also called B4) is a type I membrane glycoprotein with a molecular
weight of 95 kDa. CD19 is involved in the regulation of B lymphocyte development,
activation and differentiation. This molecule is expressed on all normal B lymphocytes
including pro-B lymphocytes, but is lost in maturation to plasma cells. It is also found on
the surface of follicular dendritic cells, on the early cells of myelomonocytic lineage and on
most stabilized B cell lines. It is not present on normal T lymphocytes, NK cells, monocytes,
and granulocytes.
CD19
lymphocytes.
Clone: J3-119 Isotype: IgG1 Mouse
In vitro studies show that the CD19 antibodies have an inhibitory effect on the activation and proliferation of B lymphocytes. They also
inhibit the B cell response after co-stimulation by anti-immunoglobulin and interleukin 4. Tissue reactivity: J3-119 reacts with B
lymphocytes, not with T lymphocytes, monocytes or granulocytes. J3-119 stains mantle-zone and germinal-center lymphoid cells, as
well as follicular dendritic cells. Plasma cells are negative. Staining pattern: membrane.
4
B I C
Part# Status
Size
Form Format Line
IOTest Liquid ASR A96418 0.5 mL Krome Orange
Clone: 89B (B4) Isotype: IgG1 Mouse
2
C I F
Part# Status
Size
Form Format Line
117
Human CDs
Clone: HD237 (B4 lytic) Isotype: IgG2b Mouse
3
C B
Part# Status
Size
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The CD20 antigen (Bp35) is an integral non-glycosylated membrane protein with four
transmembrane domains. Three isoforms (33, 35 and 37 kDa) result from differential
phosphorylation within the cytoplasmic domain. The CD20 molecule is present on all
normal B lymphocytes from peripheral blood, lymph node, spleen, tonsil and bone marrow
but is absent from plasma cells. It is expressed naturally but at low density on a subset of
peripheral blood T lymphocytes.
CD20
Clone: H299 (B1) Isotype: IgG2a Mouse
1
C F I B E
Part# Status
Size
Form Format Line
Clone: B9E9 (HRC20) Isotype: IgG2a Mouse
All monoclonal antibodies directed to the extracellular domain of the CD20 molecule are likely to bind closely related epitopes. Staining
pattern: membrane.
5
C
Part# Status
Size
Form Format Line
- Liquid RUO IM1456 1 mg Purified
IOTest Liquid CE A21693 100 tests APC
118
Human CDs
Clone: FMC7 Isotype: IgM Mouse
The FMC7 antibody reacts with a subpopulation of peripheral blood B lymphocytes and tonsil B cells, but it does not react with
granulocytes, monocytes, platelets, erythrocytes, T lymphocytes or null cells.
W C
Part# Status
Size
Form Format Line
IOTest Liquid ASR IM1364U FITC
The CD21 antigen is a 145 kDa transmembrane glycoprotein. It belongs to the gene family of complement regulatory proteins.
CD21 antigen (CR2) is the receptor for C3d and for Epstein-Barr virus (EBV). CD21 is present on mature B lymphocytes. It is
absent from T lymphocytes, granulocytes and monocytes. This antigen is involved in B-lymphocyte activation and proliferation.
CD21
Clone: B120 (B2) Isotype: IgM Mouse
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Tissue reactivity: BL13 reacts with mature B lymphocytes and follicular dendritic cells. It stains the center of the secondary lymphoid
follicles of lymph nodes and tonsils. Staining pattern: membrane.
3
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IOTest Liquid ASR A32536 2 mL PE
119
Human CDs
The CD22 antigen (BL-CAM) is a transmembrane glycoprotein of the immunoglobulin
superfamily. It may be expressed as two different isoforms: the form (130 kDa) and the
form (140 kDa). CD22 antigen is a B cell restricted antigen expressed in the cytoplasm of
B cell progenitors and on the surface of mature B lymphocytes. CD22 expression is lost
after B-cell activation, prior to the plasma cell stage. It is absent from peripheral blood T
lymphocytes, granulocytes, monocytes.
CD22
Clone: HD239 (B3) Isotype: IgG2b Mouse
The B3 antibody binds to an epitope located within the first Ig-like domain of CD22 antigen. It can be used to detect intracytoplasmic
CD22 in flow cytometry after permeabilization. Staining pattern: membrane; cytoplasmic in early B cells.
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Clone: SJ10.1H11 Isotype: IgG1 Mouse
Tissue reactivity: SJ10.1H11 strongly stains mantle zone B cells and weakly the germinal center cells. Staining pattern: membrane;
cytoplasmic in early B cells.
3
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120
Human CDs
The CD23 antigen (also called B6) is a 45 kDa transmembrane glycoprotein associated
with MHC Class II antigen, which belongs to the C-type lectin family. CD23 is the low
affinity receptor for IgE (FcRII). CD23 antigen is expressed on B lymphocytes, monocytes
and follicular dendritic cells (FDC). Expression of CD23 by B lymphocytes is up-regulated
following mitogen or antigen activation. Soluble forms of CD23 are generated by
proteolytic cleavage of the membrane molecule.
CD23
lymphocytes.
Clone: HD50 (B6) Isotype: IgG2b Mouse
3
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COULTER CLONE Liquid ASR 6604368 1 mL FITC
Clone: 9P25 Isotype: IgG1 Mouse
The 9P25 monoclonal antibody does not recognize T cells, myeloid cells, erythrocytes or platelets. 9P25 blocks the binding of IgE.
Tissue reactivity: 9P25 reacts with mature B cells and monocytes, especially with mantle zone B cells. It also stains T cells, eosinophils,
platelets, Langerhans cells and a subset of thymic epithelial cells. Staining pattern: membrane.
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121
Human CDs
The CD24 antigen is a glycosyl-phosphatidylinositol-linked membrane sialoglycoprotein,
with a molecular weight of 35-45 kDa. CD24 is present on B cells, from the pre-B to the
mature B cell stage, but not on plasma cells. It is expressed on mature granulocytes and
on a variety of epithelial cell types.
CD24
Tissue reactivity: ALB9 reacts with B cells from pre-B to mature B stages except plasma cells. It also stains neutrophils, activated T
cells and interdigitating reticulum cells. Staining pattern: membrane.
6
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122
Human CDs
The CD25 antigen (IL-2R chain, Tac or p55) is a single-chain glycoprotein with a molecular weight of 55 kDa which is the
low affinity receptor for IL-2 (IL-2R). In conjunction with CD122 (IL-2R, p75) and CD132 (IL-2R, p64), CD25 antigen
forms the high-affinity IL-2R complex. CD25 antigen is found on activated T and B cells, on activated monocytes and
macrophages, and on T cell clones. It is also expressed on NK cells enriched from peripheral blood lymphocytes by culturing in
the presence of IL-2. It is absent from thymocytes and resting T and B lymphocytes.
CD25
Clone: 1HT44H3 (IL-2R1) Isotype: IgG2a Mouse
2
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Clone: B1.49.9 Isotype: IgG2a Mouse
The B1.49.9 monoclonal antibody does not block the binding of IL-2 to its receptor. Staining pattern: membrane.
2
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The CD26 antigen is a single-chain glycoprotein with a molecular weight of 110 kDa. It
binds to adenosine deaminase (ADA) and is a functional collagen receptor. It is identical to
dipeptidylpeptidase IV ectoenzyme (DPPIV). This antigen is expressed by activated T
lymphocytes and B lymphocytes and by macrophages.
CD26
Clone: 4EL-1C7 (Ta1) Isotype: IgG1 Mouse
The 4EL-1C7 monoclonal antibody does not inhibit T cell activation and cytotoxicity.
2
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IOTest Liquid ASR IM2724 2 mL PE
123
Human CDs
The CD27 antigen is a transmembrane glycoprotein disulfide-linked homodimer composed
of 55 kDa monomers. CD27 belongs to the Tumor Necrosis Factor Receptor (TNFR) gene
family. CD27 antigen is found on medullary thymocytes, peripheral T lymphocytes,
activated B lymphocytes and NK cells. It is preferentially expressed on the CD45RA
+
CD45R0
-
, nave subset of CD4
+
T lymphocytes. Its ligand is CD70.
CD27
lymphocytes.
Clone: 1A4CD27 Isotype: IgG1 Mouse
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The CD28 antigen (Tp44) is a homodimer of disulfide-linked chains, with a molecular
weight of 44 kDa each. CD28 antigen is expressed by a majority of CD3
+
T lymphocytes.
CD4
+
T lymphocytes express the CD28 antigen more frequently than do CD8
+
T
lymphocytes. CD28 antigen is also present on plasma cells and thymocytes. This antigen
is involved in the adhesion of T lymphocytes with B lymphocytes, through the B7/BB-1
(CD80) or B70/B7-2 (CD86) glycoproteins and provides a major co-stimulatory signal for
T cell activation.
CD28
Clone: CD28.2 Isotype: IgG1 Mouse
The CD28.2 monoclonal antibody costimulates T cell proliferation and cytokine production in the presence of T cell activators (CD3,
PMA, SEB). It inhibits proliferation of CD4
+
T lymphocytes. It cross-reacts with Rhesus monkey peripheral blood lymphocytes.
5
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- Liquid ASR IM1235 2 mL Purified
124
Human CDs
The CD29 antigen is the 130 kDa integrin 1 chain which is expressed as a heterodimer,
non-covalently associated with the integrin subunits 1 to 6 (CD49a to CD49f) also
known as Very Late Antigens (VLA). CD29 is also known as platelet GPIIa. The antigen
mediates cellular adhesion and has broad cellular reactivity, but is not expressed by
erythrocytes. In normal peripheral blood it is found on a subset of CD4
+
and CD8
+
T
lymphocytes and on a minority of B lymphocytes. The "inducer" subpopulation of CD4
+
lymphocytes co-expresses CD4 and CD29.
CD29
Clone: 4B4LDC9LDH8 (4B4) Isotype: IgG1 Mouse
4B4LDC9LDH8 is a blocking monoclonal antibody in CD29 mediated adhesion. 4B4LDC9LDH8 was used as CD29 reference monoclonal
antibody during HLDA 6.
3
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COULTER CLONE Freeze-dried RUO 6603113 100 tests Purified
COULTER CLONE Freeze-dried RUO 6603109 100 tests FITC
CYTO-STAT Liquid RUO 6604105 50 tests FITC
CYTO-STAT Liquid RUO 6604159 50 tests RD1
Clone: K20 Isotype: IgG2a Mouse
K20 is a non blocking monoclonal antibody in CD29 mediated adhesion. Tissue reactivity: K20 reacts with nearly all tissue and cell types
except red blood cells. Staining pattern: membrane.
3
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Clone: Lia1/2 Isotype: IgG1 Mouse
The Lia1/2 monoclonal antibody induces cell aggregation.
3
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125
Human CDs
The CD30 antigen is a single-chain glycoprotein with a molecular weight of 105 kDa (Ki-1
antigen). It is a member of the TNFR (Tumor Necrosis Factor Receptor) superfamily and
binds to CD153 (CD30 ligand). CD30 antigen is found on activated T and B lymphocytes
and on Reed Sternberg cells.
CD30
Large anaplastic cell lymphoma (AEC)
Clone: HRS4 Isotype: IgG1 Mouse
HRS4 recognizes a formaldehyde-resistant epitope. Tissue reactivity: on paraffin sections HRS4 reacts with activated T and B cells at
the rim of germinal centers and in interfollicular areas in most lymphoid tissues. Occasionally, plasma cells, immature erythroblasts and
megakaryocytes may be stained by HRS4. Staining pattern: membrane with dot-like labeling in the Golgi region.
3
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The CD31 antigen, also known as Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a transmembrane glycoprotein
of 130 kDa related to the immunoglobulin superfamily. Its expression has been shown on stem cells of the myeloid lineage, on
platelets and on endothelial cell junctions. CD31 has been demonstrated to bind in a homophilic manner (CD31 binds CD31
on opposing cells). It also mediates heterophilic interactions, in particular, CD31 is involved in the migration of leucocytes
through the endothelial cell wall, via adhesion to v3 integrin and to CD38. In addition, CD31 participates in outside-in
signaling in leucocytes likely through phosphorylation of its intracytoplasmic tyrosine residues 663 and 686 and subsequent
association with tyrosine phosphatases SHP-1 and SHP-2. Studies suggest a comparable mechanism of action for CD31 in
activation and aggregation of platelets.
CD31
Clone: 1F11 Isotype: IgG1 Mouse
5
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IOTest Liquid RUO IM2409 100 tests PE
Clone: 5.6E Isotype: IgG1 Mouse
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126
Human CDs
FcRII proteins exist as six potential isoforms encoded by three genes, designated as A, B,
and C. The reported molecular weight of these isoforms are variable between 37 and 43
kDa. All isoforms are similar but not identical in their extracellular regions. The cytoplasmic
portion of FcRIIA and FcRIIC contains an activatory ITAM motif whereas FcRIIB
contains an inhibitory ITIM motif. Differential splicing of FcRIIB gene yields to 2 isoforms
FcRIIBb1 and FcRIIBb2. Monocytes carry all forms of FcRII, neutrophils carry FcRIIA
and C. FcRIIB is expressed by B lymphocytes and mast cells.
CD32
Clone: 2E1 Isotype: IgG2a Mouse
The 2E1 antibody recognizes the antigen isoforms expressed on basophils, neutrophils, eosinophils, platelets and B cells.
3
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127
Human CDs
The CD33 antigen is a 67 kDa single-chain transmembrane glycoprotein reported to be a
sialic acid dependent adhesion molecule. The extracellular portion of the molecule belongs
to the Ig superfamily, involving one V-like and one C2-like domains. This antigen shows
some homologies to myelin-associated glycoprotein and to the CD22 molecule. CD33
antigen is expressed on monocytes in peripheral blood. In the bone marrow, it is found on
granulocyte and macrophage precursors, and is absent from pluripotent stem cells.
CD33
Clone: 906 (MY9) Isotype: IgG2b Mouse
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Clone: D3HL60.251 Isotype: IgG1 Mouse
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128
Human CDs
The CD34 antigen is a monomeric transmembrane phosphoglycoprotein of about 110 kDa
with two distinct extracellular domains. The membrane proximal domain, of about 110
amino acids, probably adopts a globular conformation. The NH2-terminal domain, of about
140 amino acids, is heavily glycosylated with both N-linked glycans and sialylated O-linked
carbohydrates and probably exhibits an extended rod-like structure typical of mucin-like
glycoproteins. The CD34 antigen is expressed on hematopoietic progenitor cells of all
lineages as well as the most primitive pluripotential stem cells. CD34 antigen expression is
highest on the most primitive stem cells and is gradually lost as lineage committed
progenitors differentiate. The CD34 antigen is also present on capillary endothelial cells
and on bone marrow stromal cells. Variations of glycosylation are thought to occur during
normal hematopoiesis depending on lineage commitment and the level of cellular
maturation.
CD34
KG1a cell line.
Clone: Immu133 Isotype: IgG1 Mouse
Epitope Class I: Immu133 monoclonal antibody detects sialic acid-dependent class I epitope.
5
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- Liquid RUO IM1167 1 mg Purified
IOTest Liquid RUO IM2649 100 tests PC5
Clone: QBEnd10 Isotype: IgG1 Mouse
Epitope Class II: QBEnd10 recognizes a Pasteurella glycoprotease-sensitive class II epitope. For quantitation of CD34
+
cells in
cytokine-mobilized peripheral blood and apheresis products, phycoerythrin conjugates of QBEnd10 or 581 are preferred. Tissue
reactivity: QBEnd10 reacts with early normal hematopoietic progenitor cells, and with vascular endothelial cells. Staining pattern:
membrane.
4
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N
- Liquid RUO IM0787 100 tests Purified
129
Human CDs
Clone: 581 Isotype: IgG1 Mouse
Epitope Class III: Clone 581 recognizes a sialidase / glycoprotease / chymopapain insensitive class III epitope.
5
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Clone: QBEnd10 + Immu133 + Immu409 Isotype: IgG1/IgG1/IgG2a
The CD34 Pool Kit is delivered with its corresponding isotypic control. Staining pattern: membrane.
Part# Status
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The CD35 antigen is a polymorphic molecule. Different molecular weights: 160, 190, 220,
250 and 285 kDa were reported for the various allotypes. It is known as the Complement
Receptor type I (CR1) with affinity for C3b, C4b and iC3b. The CD35 antigen is
expressed by granulocytes (except basophils), B lymphocytes, monocytes, some NK cells
and erythrocytes. The CD35 antigen mediates phagocytosis of complement coated
particles.
CD35
Clone: J3D3 Isotype: IgG1 Mouse
The J3D3 monoclonal antibody inhibits CR1-mediated decay of cells bound to the alternative and classical pathways to C3-convertase
sites. It also inhibits C3b dependent rosette formation with lymphocytes. Tissue reactivity: J3D3 reacts with B lymphocytes, monocytes,
myeloid cells, dendritic cells of reactive follicles and renal podocytes. Staining pattern: membrane.
3
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IOTest Liquid RUO IM1836 100 tests FITC
130
Human CDs
The CD36 antigen (platelet GPIV) is a generic term for a family of glycoproteins with molecular weights ranging from 78 to 88
kDa. This molecule is found on platelets, endothelial cells, monocytes, and some human tumor cell lines. It is a very early marker
of erythroid differentiation and mediates the adherence of platelets to thrombospondin and collagen.
CD36
Clone: FA6.152 Isotype: IgG1 Mouse
The FA6-152 monoclonal antibody, raised against fetal erythrocytes, recognizes the CD36 family of antigens on platelets and certain
hematopoietic cells. It does not react with lymphocytes or granulocytes. It reacts with both fetal and adult monocytes, megakaryocytes,
platelets, and with reticulocytes.
5
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IOTest Freeze-dried ASR A87786 50 tests APC
The CD37 antigen is a 40-52 kDa single chain transmembrane protein with 4 transmembrane domains (Tetraspan/TM4SF).
This molecule has both its NH
2
- and COOH-termini inside the cells, and is heavily glycosylated on one extracellular loop. The
main feature of this antigen is its high content of hydrophobic residues (45%). The CD37 antigen is strongly expressed on the
surface of human peripheral blood B lymphocytes and on B lymphocytes from germinal centers. In the ontogeny of B cells, this
antigen appears late, after the pre-B stage, and is not expressed on plasma cells. The CD37 antigen has a low expression on T
lymphocytes, granulocytes, and monocytes. A weak reactivity is observed with other cellular types such as Langerhans cells,
macrophages, normal medullar granulous cells and peripheral blood polymorphonuclear cells. CD37 is involved in signal
transduction.
CD37
Tissue reactivity: BL14 reacts with B lymphocytes in germinal center, follicular mantle and extrafollicular zones, and not with plasma
cells. It weakly reacts with T cells, monocytes and neutrophils. Staining pattern: membrane.
3
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IOTest Liquid ASR IM0457 2 mL FITC
131
Human CDs
The CD38 antigen is a 45 kDa single chain type II integral membrane glycoprotein, with
the NH
2
-terminus inside the cytoplasm. CD38 is an enzyme with several activities such as
NAD glycohydrolase, ADP ribosylcyclase and cyclic ADP ribose hydrolase. CD38 is
expressed on activated T and B lymphocytes, NK cells, monocytes, plasma cells and
medullary thymocytes. CD38 expression appears to depend on the differentiation and
activation of the cell. In the B cell lineage, CD38 is expressed in early stages of B-cell
ontogeny, lost during maturation and re-expressed upon terminal differentiation to plasma
cells. Similarly, CD38 is expressed on thymocytes and at a high level on activated T cells.
Most mature resting lymphocytes of both B and T lineages do not express the CD38
antigen. CD38 is widely used as a marker to study T and B lymphocyte activation.
CD38
Activated PBL.
Clone: T16 Isotype: IgG1 Mouse
Tissue reactivity: T16 monoclonal antibody reacts with plasma cells, early B lymphocytes and activated T lymphocytes. Staining
pattern: membrane.
3
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Clone: LS198-4-3 Isotype: IgG1 Mouse
5
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IOTest Liquid ASR A99022 0.5 mL ECD
IOTEST Liquid ASR A54189 0.5 mL PC7
132
Human CDs
The CD40 antigen is a glycosylated phosphoprotein. It is a member of the Tumor Necrosis
Factor Receptor (TNFR) superfamily and contains four cysteine-rich repeats. Its molecular
weight is about 44-48 kDa. CD40 antigen is present on all mature B lymphocytes, but not
on plasma cells. It is strongly expressed by interdigitating cells, some epithelial cells,
follicular dendritic cells, and fibroblasts and macrophages. CD40 binds to CD154 (CD40
ligand) on T cells, resulting in B cell growth and differentiation.
CD40
Clone: MAB89 Isotype: IgG1 Mouse
Co-culture with MAB89 and IL-4 leads to long term B cell proliferation. Tissue reactivity: MAB89 reacts with all mature B lymphocytes
(not with plasma cells), with basal and some epithelial cells, interdigitating cells, follicular dendritic cells and macrophages. Staining
pattern: membrane.
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133
Human CDs
The CD41 antigen (platelet GPIIb; IIb integrin) is a transmembrane glycoprotein composed
of two chains GPIIb (120 kDa) and GPIIb (23 kDa) linked by one disulfide bond. CD41
is always non-covalently associated with CD61 (platelet GPIIIa, 3 integrin), to form the
GPIIb-IIIa (CD41/CD61) complex. CD41 is expressed by platelets, megakaryocytes and a
small subset of CD34
+
cells, suggesting that CD41/CD61 is one of the earliest markers of
the megakaryocytic lineage. The resting form of the CD41/CD61 complex binds to
immobilized fibrinogen and upon platelet activation, the complex becomes a receptor for
soluble fibrinogen, fibronectin, vWF, vitronectin and thrombospondin. It is involved in
platelet aggregation.
CD41
Isolated platelet rich plasma from a normal
whole blood sample.
Clone: 69 (Plt-1) Isotype: IgM Mouse
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Clone: P2 Isotype: IgG1 Mouse
The P2 antibody reacts with the chain of CD41a (GPIIb) in the intact complex with GPIIIa but not with the GPIIb or GPIIIa
separately. This antibody blocks binding to fibrinogen and inhibits platelet aggregation induced by thrombin, collagen and ADP. The P2
antibody was used as a CD41 reference mAb during HLDA 6.
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Clone: SZ22 Isotype: IgG1 Mouse
The SZ22 monoclonal antibody reacts with the chain of CD41 (GPIIb) on platelets and megakaryocytes. This monoclonal does not
inhibit platelet aggregation and secretion induced by collagen, arachidonic acid, and thrombin. It does not inhibit fibrinogen binding to
human platelets induced by ADP, arachidonic acid and PAF.
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134
Human CDs
The CD42a antigen (GPIX) is a 22 kDa single-chain membrane glycoprotein that forms a
noncovalent complex with CD42b, CD42c and CD42d (GP1X, GP1b, GPV). The CD42a-
d complex is the receptor for von Willebrand's factor and is known as von Willebrand's
factor-dependent adhesion receptor. The CD42a antigen is expressed on platelets and
megakaryocytes.
CD42a
Platelet rich plasma from a normal whole
blood sample.
Clone: SZ1 Isotype: IgG2a Mouse
The SZ1 monoclonal antibody reacts with the CD42a-d complex but does not recognize GPIb or GPIX individually.
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The CD42b antigen (GPIb) is a membrane glycoprotein of 135 kDa, that forms a
disulfide linked 160 kDa membrane protein with the CD42c (GP1b) known as GP1b.
GP1b forms a noncovalent complex with GPIX (CD42a) and GPV (CD42d). This antigen
is found on platelets and megakaryocytes. It has also been reported on vascular and
tonsillar endothelial cells. The CD42a-d complex is the receptor for von Willebrand's factor
and is known as von Willebrand's factor-dependent adhesion receptor.
CD42b
Platelet rich plasma from a normal whole
blood sample.
The SZ2 monoclonal antibody prevents the ristocetin-dependent binding of von Willebrand's factor to platelets and inhibits ristocetin-
induced platelet agglutination. Platelet aggregation induced by type I collagen and Platelet Activating Factor (PAF) is also inhibited. The
SZ2 antibody immunoprecipitates the components of the GPIb complex. It may also be used in Western blot.
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135
Human CDs
The CD43 antigen is a 95-135 kDa heavily O-glycosylated and sialylated single chain membrane glycoprotein. This cell
surface mucin-type glycoprotein is also called leukosialin or sialophorin. CD43 antigen is expressed by platelets and by all
leucocytes, weakly on resting B lymphocytes but highly after activation. A soluble form of CD43 (galactoglycoprotein) can be
detected in plasma. This form is produced by proteolytic shedding of the extracellular portion of the membrane molecule after
cell activation.
CD43
Clone: DFT1 Isotype: IgG1 Mouse
The DFT1 antibody reacts with an extracellular epitope of the CD43 antigen. It is useful for detecting leucocytes by flow cytometry and
immunohistochemistry on routinely-fixed and parrafin-embedded tissue sections. The DFT1 antibody is also able to induce homotypic
adhesion of leucocytes. Tissue reactivity: DFT1 reacts with leucocytes except non-activated B lymphocytes. Staining pattern:
membrane.
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The CD44 antigens are transmembrane glycoproteins and members of the hyaladherin family of hyaluronan-binding proteins.
Multiple CD44 isoforms have been described, the predominant form being CD44S, a glycoprotein of 85 kDa. CD44 is present
on most cells or tissues, but not on platelets, hepatocytes, cardiac muscle, kidney tubular epithelium, testis and skin portions.
The human blood group antigens Ina/b reside on CD44.
CD44
Clone: J.173 Isotype: IgG1 Mouse
J.173 antibody does not inhibit the binding of hyaluronate to its receptor. It can trigger IL-2-dependent proliferation and cytotoxicity of
human T cell clones in vitro.
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136
Human CDs
The CD45 molecules are single chain integral membrane proteins comprising at least five
isoforms ranging from 180 to 220 kDa. They are generated by alternative splicing
combinations of three exons (A, B, and C) of the genomic sequence. The non-restricted
CD45, Leucocyte Common Antigen (LCA) consists of an extracellular sequence, proximal
to the membrane, which is common to all CD45 isoforms. All the monoclonal antibodies
that belong to the CD45 cluster react with this part of the antigen and are able to
recognize all CD45 isoforms. These isoforms have extracytoplasmic sequences ranging
from 391 to 552 amino acids long with numerous N-linked carbohydrate attachment sites.
The cytoplasmic portion contains two phospho-tyrosine-phosphatase domains. The non-
restricted CD45 epitope is present on the surface of all human leucocytes, lymphocytes,
eosinophils monocytes, basophils and neutrophils by order of decreasing level of
expression. CD45 is a major component of the lymphocyte membrane. It is absent from
erythrocytes and platelets. It is lost during maturation of erythroid cells in the bone marrow.
CD45 antibodies react with leucocyte progenitors in bone marrow.
CD45
Clone: J.33 Isotype: IgG1 Mouse
J.33 was assigned to CD45 cluster in HLDA 3 under the name I.33 (antibody # 818) Staining pattern: mainly membrane but weak
cytoplasmic staining can also be observed.
3
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IOTest Liquid ASR A94245 1 mL Alexa Fluor* 488
IOTest Liquid CE A62835 100 tests PC5.5
IOTest Liquid ASR A54139 1 mL PC5.5
IOTest Liquid ASR A94246 1 mL Alexa Fluor* 647
IOTest Liquid ASR A71117 1 mL APC-Alexa Fluor* 700
IOTest Liquid ASR A71119 1 mL APC-Alexa Fluor* 750
IOTest Liquid ASR A74765 1 mL Pacific Blue*
IOTest Liquid CE A74763 100 tests Pacific Blue*
IOTest Liquid ASR A96416 1 mL Krome Orange
137
Human CDs
Clone: KC56 (T-200) Isotype: IgG1 Mouse
Staining pattern: mainly membrane but weak cytoplasmic staining can also be observed.
5
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Clone: Immu19.2 Isotype: IgG1 Mouse
Staining pattern: mainly membrane but weak cytoplasmic staining can also be observed.
6
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The ALB12 antibody recognizes a carbohydrate epitope and should not be used for side scatter versus CD45 gating strategy in flow
cytometry. Staining pattern: mainly membrane but weak cytoplasmic staining can also be observed.
C
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Clone: Ros220 + ALB12 Isotype: IgG1/IgG2a Mouse
Tissue reactivity: the Ros220 + ALB12 pool strongly stains lymphoid cells, but histiocytes and macrophages to a variable degree. All
non-hematopoietic tissues are negative. Staining pattern: mainly membrane but weak cytoplasmic staining can also be observed.
Part# Status
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138
Human CDs
The CD45R0 molecule is the 180 kDa isoform of CD45 Leucocyte Common Antigen
(LCA). The expression of the CD45RA/CD45R0 isoforms of CD45 is associated with
functional differentiation of T lymphocytes. CD45R0 is expressed by cortical thymocytes,
CD4
+
memory T lymphocyte subset and activated T lymphocytes. CD45R0 is weakly
expressed on monocytes and granulocytes.
CD45R0
lymphocytes.
Clone: UCHL1 Isotype: IgG2a Mouse
Tissue reactivity: UCHL1 reacts with most thymocytes, a portion of mature T lymphocytes and activated T cells. It also stains
granulocytes and monocytes, mature myeloid cells and a subset of macrophages. It does not react with non-lymphoid tissue. Staining
pattern: membrane.
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139
Human CDs
CD45RA epitopes are present on all the CD45 restricted molecules in which the exon A is
expressed (ABC and AB containing isoforms). Most B lymphocytes express CD45RA
isoforms. The differential expression of CD45 isoforms is associated with different
functional properties of T lymphocytes. Peripheral T lymphocytes can be divided into two
largely distinct populations of CD45RA
-
CD45RO
+
and CD45RA
+
CD45RO
-
cells.
CD45RA is expressed by medullar thymocytes and nave/resting peripheral CD4
+
T
lymphocytes.
CD45RA
lymphocytes.
Clone: 2H4LDH11LDB9 (2H4) Isotype: IgG1 Mouse
3
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CYTO-STAT Liquid RUO 6603904 50 tests FITC
CYTO-STAT Liquid RUO 6603866 50 tests RD1
IOTEST Liquid ASR B10821 0.5 mL PC7
IOTEST Liquid ASR A86050 0.5 mL APC-Alexa Fluor* 750
IOTEST Liquid ASR A82946 0.5 mL Pacific Blue*
The ALB11 antibody reacts with B lymphocytes, monocytes and granulocytes. The coexpression of CD4 and CD45RA allows the
identification of the suppressor-inducer subpopulation of CD4 lymphocytes. Tissue reactivity: ALB11 reacts with significant portions of
peripheral CD4
+
T cells, peripheral CD8
+
T lymphocytes, B lymphocytes and monocytes. Staining pattern: membrane.
5
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Clone: DBB42 Isotype: IgG1 Mouse
Tissue reactivity: DBB42 reacts with some macrophages and T cells of the red pulp. In the thymus, a few medulary lymphocytes are
stained. DBB42 reacts with almost all B-cell lymphomas and with up to 10% of T-cell lymphomas. Staining pattern: membrane.
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Human CDs
The CD46 antigen is a 56-66 kDa non-disulfide-linked dimeric protein with O-linked glycosylation. It is known as the
Membrane Co-factor Protein (MCP). This cofactor binds to C3b or C4b, allowing their degradation by a serine protease of the
plasma (factor I). CD46 is the receptor for the measles virus hemagglutinin and Streptococcus A surface protein. CD46
antigen is expressed on thymocytes, T lymphocytes, B lymphocytes, monocytes, granulocytes, NK cells, platelets, endothelial
cells, epithelial cells, and fibroblasts, but not on erythrocytes.
CD46
Clone: J4.48 Isotype: IgG1 Mouse
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The CD48 antigen is a glycosyl phosphatidylinositol- (GPI)-anchored membrane glycoprotein of 40-47 kDa with 2 extracellular
immunoglobulin (Ig)-like domains. CD48 belongs to the Ig superfamily. CD48, expressed on all lymphocytes and monocytes,
has been shown to be a low affinity ligand for CD2, suggesting a role in leucocyte adhesion. CD48 may participate in CD40-
mediated activation of B cells.
CD48
Clone: J4.57 Isotype: IgG1 Mouse
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The CD49b antigen is a transmembrane glycoprotein of 150 kDa also known as the -2 integrin and as platelet GPIa. CD49b
non-covalently pairs with the -1 integrin (CD29, GPIIa) to form the VLA-2 (very late activation antigen) complex. CD49b
complexed to CD29 is involved in cell adhesion to collagen. It is expressed by platelets, activated T lymphocytes and
thymocytes.
CD49b
Clone: Gi9 Isotype: IgG1 Mouse
The Gi9 antibody reacts with the molecule carrying the Br(a) as well as the Br(b) alloantigen. This antibody inhibits the adhesion of
platelets to collagen. It detects human alloantibodies (anti-Br a,b) against VLA-2 by MAIPA assay. Gi9 was used as a CD49b reference
monoclonal antibody during the HLDA 6. Gi9 reacts with platelets and weakly with monocytes. It also stains most adherent cell lines
and T lymphocytes in culture. Staining pattern: membrane.
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Human CDs
The CD49c antigen is the 150 kDa integrin 3 chain which is non-covalently associated with the 130 kDa integrin 1-chain
(CD29 molecule) forming the VLA-3 complex (31 or CD49c/CD29). It is known as the receptor for laminin, collagen,
fibronectin, invasin and epiligrin.
CD49c
Clone: C3VLA3 Isotype: IgG1 Mouse
The C3VLA3 antibody recognizes an amino-terminal epitope of the integrin 3 chain and immunoprecipitates the 120-130 kDa fragment
under reducing conditions. This antibody reacts with several human cells including some peripheral blood lymphocytes and high
passage cultured human umbilical vein endothelial cells (HUVECs). It does not react with monocytes and granulocytes. Tissue
reactivity: the C3VLA3 antibody reacts with smooth muscle cells of some blood vessels in normal human breast, kidney and liver and
basal cells of the epidermis. It does not react with skeletal and cardiac muscle cells. Staining pattern: membrane.
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CD49d is the integrin 4 chain that non-covalently pairs with CD29 (1 chain) to form the very late activation antigen 4 (VLA
-4), or with the integrin 7 chain. CD49d is a transmembrane glycoprotein of 145 kDa devoid of disulfide bond. Complexed to
1 or 7 integrins, CD49d is not only involved in cell adhesion to fibronectin and vascular cell adhesion molecule 1 (VCAM-1),
but also in intercellular leucocyte interactions. It is expressed by monocytes, lymphocytes, eosinophils, thymocytes and
Langerhans cells.
CD49d
Clone: HP2/1 Isotype: IgG1 Mouse
The HP2/1 antibody blocks the interaction of VLA-4 with VCAM-1. HP2/1 was used as a CD49d reference monoclonal antibody during
the HLDA 6. Tissue reactivity: HP2/1 stains most fresh or cultured lymphocytes, thymocytes, monocytes and eosinophils. Staining
pattern: membrane.
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The CD49e antigen is the 160 kDa integrin 5-chain, which is composed of a heavy chain (135 kDa) and a light chain (25
kDa), linked by a disulfide bond. CD49e is non-covalently associated with the 130 kDa integrin 1-chain (CD29 molecule),
forming the VLA-5 complex. CD49e is expressed by monocytes, platelets and two myeloid cell lines (U937, K562). It is known
as the fibronectin and invasin receptor. It is useful for basic studies of fibronectin-mediated adhesion.
CD49e
Clone: SAM1 Isotype: IgG2b Mouse
Tissue reactivity: the SAM1 antibody strongly reacts with monocytes, and weakly with granulocytes, platelets and T lymphocytes. In
normal tissues, SAM1 stains endothelial cells. Staining pattern: membrane.
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Human CDs
The CD49f antigen (GPIc, or integrin 6-chain) is composed of a large subunit of 125 kDa and a small transmembrane,
subunit of 25 kDa, which are bridged together through a disulfide link. When non-covalently associated with the 130 kDa
integrin 1-chain (CD29 molecule), it forms the VLA-6 complex (GPIc/IIa, or 61). 61 is known as the laminin receptor.
CD49f is found on platelets and megakaryocytes. It is weakly expressed by a subpopulation of T lymphocytes.
CD49f
Clone: GoH3 Isotype: IgG2a Rat
The GoH3 antibody inhibits the adhesion of platelets to laminin. It is not suitable for Western blot analysis. Tissue reactivity: the GoH3
antibody strongly reacts with platelets and moderately stains thymocytes, monocytes and T lymphocytes. Most cultured adherent cells
also express CD49f. Staining pattern: membrane.
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The CD50 antigen is a highly glycosylated type I membrane protein of 120-130 kDa. CD50 is expressed by leucocytes of all
lineages, with or without activation, but not by platelets or erythrocytes. It is known as the Inter-Cellular Adhesion Molecule-3
(ICAM-3) and interacts with LFA-1 in cellular adhesion.
CD50
Clone: HP2/19 Isotype: IgG2a Mouse
The HP2/19 antibody strongly blocks adhesion which is directly or indirectly mediated by ICAM-3. This antibody has a co-stimulatory
effect in CD3-dependent proliferation. HP2/19 was used as a CD50 reference monoclonal antibody during the HLDA 6.
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The CD51 antigen is the 140 kDa integrin V-chain, consisting of 2 subunits of 125 kDa and 25 kDa respectively, linked
together through a disulfide link. The V-chain can be non-covalently associated with the integrin 3 chain also known as
GPIIIa or CD61 molecule. This V3 complex is known as the receptor for vitronectin, fibrinogen, thrombospondin and the von
Willebrand factor. CD51 is expressed by platelet, megakaryocytes, some B lymphocytes and by endothelial cells.
CD51
Clone: AMF7 Isotype: IgG1 Mouse
The AMF7 antibody is weakly blocking. Tissue reactivity: in combination with 3, CD51 is found on endothelial cells, some B
lymphocytes, monocytes, macrophages, platelets, osteoclasts, and mast cells. In combination with 5, CD51 is found on carcinoma
cells, hepatoma cells and fibroblasts. Smooth muscle cells and nerve tracts express CD51. AMF7 also stains colon carcinoma and breast
epithelial cells. Staining pattern: membrane.
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Human CDs
The CD54 antigen, previously known as ICAM-1 (InterCellular Adhesion Molecule-1), is a
90 kDa transmembrane glycoprotein. With seven potential N-linked glycosylation sites, its
structure, as that of CD50 (ICAM-3) and CD102 (ICAM-2), is related to the
Immunoglobulin family. The CD54 antigen mediates the adhesion of T lymphocytes with
Antigen-Presenting Cells (APC) and is involved in T cell to T cell and T cell to B cell
interactions. It is also involved in the adhesion of monocytes, lymphocytes and neutrophils
to activated endothelium. It binds to the integrins LFA-1 (CD11a/CD18) and Mac-1
(CD11b/CD18). It is a receptor for the rhinovirus. It is expressed by endothelial cells and
by many other cell types upon activation.
CD54
Clone: 84H10 Isotype: IgG1 Mouse
84H10 antibody inhibits ICAM-1 mediated adhesion to LFA-1. Tissue reactivity: CD54 is expressed by endothelial cells and monocytes,
and upregulated on B and T lymphocytes, thymocytes, dendritic cells, endothelial cells, fibroblasts, keratinocytes, chondrocytes and
epithelial cells. Staining pattern: membrane.
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The CD55 antigen is a single chain glycosyl-phosphatidyl-inositol (GPI)-anchored, cell
surface protein. This molecule undergoes post-transcriptional and post-translational
modification resulting in a heterogeneous molecular weight ranging from 55 to 80 kDa.
The CD55 antigen, also named Decay Accelerating Factor (DAF) is involved in the
regulation of both alternative and classical complement pathways. C3b/C3Bb and
C4b/C4b2a convertases, as well as echovirus, coxsackie B virus and CD97 are reported
to interact with CD55. Like other GPI-anchored proteins, CD55 is associated in the
cytoplasmic compartment with tyrosine kinases allowing signal transduction. The CD55
molecule is widely expressed on human cells including leucocytes, erythrocytes and
platelets. CD55 is weakly expressed on NK cells.
CD55
Erythrocytes.
Clone: JS11KSC2.3 Isotype: IgG1 Mouse
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Human CDs
The CD56 antigen is a 140 kDa isoform of the Neural Cell Adhesion Molecule (N-CAM).
Post-translational modifications to the polypeptide include N- and O- glycosylations,
acylation, sulphation and phosphorylation. The different N-CAM isoforms have molecular
weights ranging from 135 to 220 kDa. The CD56 antigen is moderately expressed on a
subpopulation of peripheral blood large granular lymphocytes and on all cells with NK
activity. It is also expressed by subsets of T lymphocytes. CD56 antibodies do not react
with granulocytes, monocytes or B cells.
CD56
Clone: N901 (NKH-1) Isotype: IgG1 Mouse
The N901 (NHK-1) antibody was used as a CD56 reference mAb during HLDA 6.
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Clone: C218 Isotype: IgG1 Mouse
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Human CDs
The CD57 antigen (HNK-1 antigen carbohydrate moiety) is an oligosaccharide with sulfated glucoronic acid residues that may
be linked to a variety of polypeptides or lipids. In peripheral blood, it is found on a subset of cells with natural killer activity. It is
confined to mononuclear cells, primarily large granular lymphocytes. Many of these CD57
+
cells co-express the CD8 antigen
and are a subset of the suppressor / cytotoxic T lymphocytes. Cord blood T cells or NK cells lack the CD57 antigen. It is not
expressed on red blood cells or on platelets but is present on some neuroectodermal tissues.
CD57
Clone: NC1 Isotype: IgM Mouse
Tissue reactivity: NC1 reacts with NK cells in lymphoid tissues. It stains prostatic glands, cellular elements of the central and peripheral
nervous system, gastrointestinal neuroendocrine cells, pancreatic islet cells, renal pelvis epithelium and some tubule cells. Staining
pattern: cytoplasm and/or membrane at the apical surface of adenomatous tumors; membrane for NK cells.
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CD58 is a glycoprotein of 65-70 kDa, either transmembrane or
glycosylphosphatidylinositol (GPI)-anchored. CD58 was first described as the lymphocyte-
function associated antigen 3 (LFA-3). LFA-3 was further shown to interact with CD2
suggesting an important role in various immunological responses via T-cell adhesion and
activation. CD58 mediates adhesion between killer and target cells, antigen presenting
cells and T cells. This antigen is widely expressed on leucocytes, erythrocytes and
endothelial cells.
CD58
Clone: AICD58 Isotype: IgG2a Mouse
The AICD58 antibody is a blocking antibody in LFA-3 mediated adhesion. This antibody was used as a CD58 reference mAb during HLDA
6. Tissue reactivity: CD58 is expressed by endothelial cells, connective tissues and virtually all hematopoietic cells. Staining pattern:
membrane.
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Human CDs
The CD59 antigen, also known as Protectin or Membrane Inhibitor of Reactive Lysis (MIRL) is a 18-20 kDa, single-chain,
glycosylphosphatidylinositol (GPI)-anchored cell surface protein. CD59 inhibits complement lysis by binding to the C5b-8 and
C5b-9 complexes and thus preventing formation of the polymeric C9 complex during the final steps of membrane attack
complex (MAC). CD59 is expressed on all haematopoietic cells and is widely expressed on cells in all tissues. Studies of the
susceptibility of HIV-1, HTLV-1 and HCMV to complement (C)-mediated lysis suggest that viruses incorporate host cell-
derived C regulatory proteins such as CD59 and CD55, a mechanism by which a variety of enveloped viruses may acquire
resistance to C-mediated lysis.
CD59
Clone: P282E Isotype: IgG2a Mouse
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The CD61 antigen (platelet glycoprotein GPIIIa) is the 110 kDa integrin 3 subunit, con-
covalently associated with the integrin IIb chain (CD41, platelet GPIIb) to form the GPIIb-
IIIa complex (IIb-3 integrin). Independently of CD41, CD61 is also associated with the
integrin V (CD51) to form the vitronectin receptor. CD41/CD61 is expressed only by
platelets and megakaryocytes, whereas CD51/CD61 is found on osteoclasts, endothelial
cells, macrophages, fibroblasts, smooth muscle, synovial lining cells and renal glomeruli.
CD61
Isolated platelets from normal whole blood
sample (platelet rich plasma).
The SZ21 antibody is specific for the PlA1, not the PlA2 form of GPIIIa. It inhibits platelet aggregation and secretion induced by
collagen, arachidonic acid, and thrombin. This antibody also inhibits fibrinogen binding to human platelets induced by ADP, arachidonic
acid and PAF. Tissue reactivity: CD61 is found in small and large vessel endothelial cells, fibroblasts and smooth muscle cells. SZ21 also
reacts with platelets and megakaryocytes, osteoclasts, mast cells, some B lymphocytes, monocytes and macrophages. Staining
pattern: membrane and cytoplasm.
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Human CDs
The CD62L antigen, also called LECAM-1, L-selectin or LAM-1, is a 75 kDa molecule. It
acts as the peripheral lymph node homing receptor. It is also involved in lymphocyte
binding to High Endothelial Venules (HEV) and in lymphocyte rolling on activated
endothelium. CD62L is expressed by B and T lymphocytes, monocytes, neutrophils,
eosinophils, and approximately half of the peripheral NK lymphocytes.
CD62L
lymphocytes.
Clone: DREG56 Isotype: IgG1 Mouse
In contrast to other antibodies recognizing this molecule, DREG56 is particularly effective in blocking CD62L mediated lymphocyte
binding to lymph node HEV. DREG56 (ref.33) was used as a CD62L reference monoclonal antibody during the HLDA 6.
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The CD62P antigen (P-selectin/GMP-140/PADGEM) is a member of the selectin family.
Like other selectins (CD62E and CD62L), CD62P contains an amino terminal lectin-like
domain, followed by an EGF domain, nine short consensus repeats (SCR), a
transmembrane domain and a short cytoplasmic domain. CD62P (140 kDa) is present in
megakaryocytes, in Weibel-Palade bodies of endothelial cells and in -granules of
platelets. CD62P is translocated to the surface membrane upon in vitro and in vivo
activation. CD62P is involved in the interaction between activated platelets and with
monocytes and neutrophils.
CD62P
Isolated platelets from normal whole blood
sample (platelet rich plasma).
Clone: CLBThromb/6 Isotype: IgG1 Mouse
The CLB-Thromb/6 antibody recognizes the boundary region between the lectin and EGF-like domains, which is preserved after platelet
fixation. It inhibits platelet adhesion to eosinophils in cell adhesion assays.
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Human CDs
The CD63 antigen, also known as lysosomal membrane associated glycoprotein 3 (LAMP3), is a member of the tetraspanin
(TM4SF) family. Nearly 20 genes encode tetraspanins whose main structural characteristics is their four transmembrane
domains. CD63, as other tetraspanins (CD9, CD81, CD82), has recently been reported as forming complexes with VLA-3 and
phosphatidylinositol 4-kinase, with VLA-6, CD11/CD18 and tyrosine kinase. CD63 was first described in granules of resting
platelets and on the surface membrane on activated platelets. CD63 was later identified as granulophysin, a platelet dense
granule glycoprotein. The molecular weight of the platelet form of CD63 ranges from 40 to 55 kDa. CD63 has been found to
be identical to the ME491 antigen expressed by melanoma cells. It is also found on monocytes / macrophages and is weakly
expressed by resting granulocytes, T lymphocytes and B lymphocytes. CD63 antigen, present in azurophilic granules of non-
stimulated neutrophils, is strongly expressed on the surface of neutrophils after activation. Studies have shown that CD63 may
be a marker for granule release. This phenomenom is also reported for basophils.
CD63
Clone: CLBGran/12 Isotype: IgG1 Mouse
The CD63 antibodies inhibit adhesion of neutrophils to activated endothelium. CLBGran/12 was used as a CD63 reference monoclonal
antibody during HLDA 6.
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The CD64 antigen (FcRI) is a glycoprotein with a molecular weight of 72 kDa. It belongs
to the Ig superfamily, consisting of three C2-like Ig domains. It is the high affinity receptor
for IgG and is able to bind monomeric human IgG1 and IgG3. This antigen is expressed on
macrophages and monocytes. It is also expressed on neutrophils when induced with
interferon (IFN-) and granulocyte colony-stimulating factor (G-CSF).
CD64
The 22 antibody shows especially high affinity binding to human mononuclear phagocytes.The epitope recognized by this antibody is
distinct from the IgG binding site.
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IOTest Liquid ASR B06025 0.5 mL PC7
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Human CDs
The CD65 antigen is analogous to the carbohydrate structure(s) recognized by CD15
antibodies. It is a type II chain fucoganglioside involving the oligosaccharide sequence :
Gal1 - 4GlcNac1 - 3Gal1 - 4GlcNac(Fuc1-3)1 - R. Granulocytes are CD65
positive and monocytes are weakly positive. It does not react with T and B lymphocytes.
CD65
granulocytes. Staining
Clone: 88H7 Isotype: IgM Mouse
88H7 antibody was re-assigned from the CD65s to the CD65 cluster during HLDA 6.
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The CD66 antigens were originally described as granulocyte-specific activation antigens,
related to the carcino-embryonic antigen (CEA) previously described on colon cancer
cells. The CEA/CD66 gene group belongs to the immunoglobulin (Ig) gene superfamily.
Among the CD66 cluster, CD66b (also known as CGM6) is a glycosyl-
phosphatidylinositol (GPI)-anchored glycoprotein of 95-100 kDa. CD66b is thought to
play a role in the regulation of the adhesive activity of CD11/CD18 via signal transduction
in neutrophils. It is strongly expressed by myelocytes, metamyelocytes, neutrophils and
polymorphonuclear neutrophils. CD66b is weakly expressed by promyelocytes.
CD66b
lymphocytes. Staining
Clone: 80H3 Isotype: IgG1 Mouse
The 80H3 antibody does not react with peripheral lymphocytes and monocytes.
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Human CDs
The CD69 antigen is a 60 kDa disulfide-linked dimeric structure, known as activation inducer molecule (AIM). This structure
contains two differentially glycosylated forms (33 and 27 kDa) of a single core protein, that are covalently associated. This
activation antigen is one of the earliest appearing cell surface glycoproteins (before other activation antigens such as IL2-R)
after T or B-lymphocyte activation but is absent in resting lymphocytes. It is also expressed by activated macrophages, NK cells
and other cell types including neutrophils, eosinophils and platelets. Its expression in vitro can be induced by different factors
such as PMA, PHA or CD3 monoclonal antibodies.
CD69
Clone: TP1.55.3 Isotype: IgG2b Mouse
The CD69 antibody is useful in combination with CD4, CD8 and CD3 antibodies for T cell activation studies. The TP1.55.3 antibody
immunoprecipitates the 60 kDa homodimer and both 27 kDa and 33 kDa subunits from activated peripheral blood lymphocytes under
reduced and non-reduced conditions.
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The CD71 antigen is known as the transferrin receptor. It mediates cellular iron uptake via internalization and recycling. It is a
type II membrane glycoprotein, characterized as a disulfide-bound dimer of two identical subunits of 95 kDa each. CD71 is
expressed on activated T and B lymphocytes, macrophages and erythroblasts. The N-Terminal, intracytoplasmic domain of
CD71 mediates rapid endocytosis and self recycling. CD71 is absent from resting blood leucocytes. Its expression is
upregulated on proliferating cells.
CD71
Clone: YDJ1.2.2 Isotype: IgG1 Mouse
Tissue reactivity: YDJ1.2.2 reacts with activated B and T cells. It also stains macrophages and proliferating cells. Staining pattern:
membrane.
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The CD77 antigen is neutral glycosphingolipid of the globoseries globotriaosylceramide (Gb3). It is known as the Pk blood
group antigen, a normal intermediate in the biosynthetic pathway of the P blood group antigen. CD77 is also present as an
antigen of germinal center B lymphocytes entering apoptosis. It is expressed by endothelial cells. Studies have shown that the
antigen may be associated with a Burkitt's Lymphoma-associated Antigen (BLA).
CD77
Clone: 38.13 Isotype: IgM Rat
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Human CDs
The CD79a molecule (MB-1, Ig) is part of the MB-1/B29 (CD79a/CD79b) disulfide-
linked heterodimer which is non-covalently associated with membrane immunoglobulins
(Igs) to build the B Cell antigen Receptor (BCR) complex. CD79a is a 40-45 kDa
glycoprotein and belongs to the Ig superfamily of surface antigens. CD79a protein is
specifically and strictly expressed throughout B lymphocyte differentiation. It appears early
in ontogeny, probably before expression of cytoplasmic -chain, and is present in the
cytoplasm of plasma cells.
CD79a
Normal whole blood sample.
Clone: HM47 Isotype: IgG1 Mouse
The HM47 antibody is recommended to be used in flow cytometry after permeabilization with saponin-based permeabilization reagent
IntraPrep. The HM47 antibody reacts with a cytoplasmic epitope of the CD79a molecule. Tissue reactivity: HM47 reacts with all B
lymphocytes from early to plasma cell stage. Staining pattern: membrane.
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The CD79b molecule (B29, Ig) is part of the MB-1/B29 disulphide-linked heterodimer
which is non-covalently associated with membrane-Igs to build the B Cell antigen
Receptor (BCR) complex.CD79b is a 33-40 kDa glycoprotein and belongs to the Ig
superfamily. The BCR complex is restricted to B cell lineage, expressed on the surface just
after the pre-B cell stage and is lost prior to the plasma cell stage.
CD79b
U266B1 cell line.
Clone: CB3-1 Isotype: IgG1 Mouse
The CB3-1 antibody reacts with the external part of the CD79b molecule and is to be used in flow cytometry. This antibody is not
suitable for intracytoplasmic staining. For this application the use of the CD79a antibody is recommended.
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Human CDs
The CD80 antigen (B7, BB1) is a highly glycosylated single-chain protein. Its extracellular domain consists of two Ig-like
domains. This 60 kDa molecule shares with CD86 the capability to be the ligand for two structurally similar molecules
expressed on T lymphocytes, CD28 and CD152 (CTLA-4). CD80 antigen is expressed on in vitro activated B lymphocytes
after 24h stimulation and at maximal level 48-72h post activation. It is not expressed on the majority of resting B cells from
peripheral blood but identifies a subpopulation of B cells that has been previously activated. The antigen is also expressed by
HTLV-1 transformed T cells and activated monocytes. CD80 binding provides co-stimulatory signals for T cell activation.
CD80
Clone: MAB104 Isotype: IgG1 Mouse
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The CD81 molecule is a single chain, 4 transmembrane domain protein (Tetraspan/TM4SF). Both the NH2- and COOH-
termini are located inside the cytoplasm, and two loops of the protein sequence are exposed extracellularly. The CD81 antigen
is not glycosylated and its molecular weight is 26 kDa. Its tissue distribution is broad. This antigen may be present in some
cases as multimolecular complexes, in association with other members of the TM4 superfamily (CD37, CD53) or, on the
surface of B cells, in association with CD19 and/or CD21 and/or MHC class II antigens. Most B lymphocytes, at all stages of
cellular differentiation, express CD81 at relatively high levels.
CD81
Clone: JS64 Isotype: IgG2a Mouse
The JS64 antibody reacts with the majority of normal lymphocytes, monocytes and eosinophils. Neutrophils and platelets are negative.
JS64 has an anti-proliferative effect on some cell lines, but does not cause apoptosis.
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Human CDs
CD83, or HB15 molecule, is a 43kDa glycoprotein member of the immunoglobulin
superfamily. It is composed of a single extracellular V-type Ig-like domain, a
transmembrane region and a 40 amino acid cytoplasmic domain. CD83 is predominantly
expressed by dendritic lineage cells including Langerhans cells, skin and peripheral blood
dendritic cells. It is also expressed by interdigitating reticulum cells present in the T cell
zones of lymphoid organs as well as being weakly expressed by some germinal center
lymphocytes. CD83 is expressed by in vitro generated dendritic cells. It is not expressed
by resting peripheral blood leucocytes at detectable levels and is only expressed at low
levels following lymphocyte activation in vitro.
CD83
K562 cell line.
Clone: HB15a Isotype: IgG2b Mouse
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CD85a antigen is mainly expressed on monocytes, macrophages, dendritic cells,
granulocytes and on a subset of T lymphocytes. Its ligand is unknown but CD85a
participates to the immune response by binding to the MHC class I molecules on antigen
presenting cells (APC) and by transducing a negative signal through ITIMs motives that
inhibits cell activation.
CD85a (ILT5)
Clone: 7H5 Isotype: IgG2a Rat
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Human CDs
CD85d (ILT4) is a member of the new immunoglobulin (Ig)-like transcripts (ILT) family of
genes located on human chromosome 19q13.4. ILT4 is also known as leucocyte Ig-like
receptor (LIR)-2 and monocyte / macrophage Ig-like receptors (MIR)-10. ILT4 is a
transmembrane protein recognized by 42D1 monoclonal antibody. The protein has
extracellular Ig-SF domains, and ITIM motifs in the cytoplasmic tail that inhibit cellular
responses by recruiting phosphatases such SHP-1. ILT4 is confined to the
myelomonocytic lineage as it is expressed on monocytes, macrophages, and dendritic
cells (DCs). ILT4 binds to HLA-G molecules with a 3- to 4-fold higher affinity than to
classical MHC class I molecules. ILT4 may modulate one or several of the antigen
presenting functions mediated by DCs, control inflammatory responses mediated by
monocytes / macrophages or inhibit their cytotoxicity. The recognition of HLA-G by ILT4
may play a role in materno-fetal tolerance as HLA-G is selectively expressed in the
trophoblast. It has been shown that recipients CD8
+
CD28
T suppressor (Ts) cells may

induce tolerance by up-regulating ITL3 and ILT4 expressions in donors dendritic cells,
rendering these antigen-presenting cells (APCs) tolerogenic.
CD85d (ILT4)
Clone: 42D1 Isotype: IgG2a Rat
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CD85j, also called ILT2, is a member of the immunoglobulin (Ig)-like transcripts (ILT) family of genes located on human
chromosome 19 (the numerical subclassification refers to the members in the order of the gene on the chromosome). It is a
110-kDa transmembrane glycoprotein. ILT2 receptor is a NK receptor involved in inhibition of NK and T cells cytolytic activity
and cytokine production. ILT2 is also known as leucocyte Ig-like receptor (LIR)-1 and monocyte / macrophage Ig-like receptor
(MIR)-7. The protein has 4 extracellular Ig-SF domains, and 4 cytoplasmic ITIM motifs in the cytoplasmic tail. Both myeloid and
lymphoid cells express ILT2. Among lymphoid cells, NK cells, T cell subsets and all peripheral B cells express ILT2. It is also
expressed on monocytes, macrophages, and dendritic cells. Ligands for CD85j are non-classical class I HLA-G molecules,
certain alleles of HLA-A and -B loci and the human cytomegalovirus gene product, UL18, a viral homolog of HLA class I
molecules. The inhibitory function of CD85j has been explored mainly in NK and T cells. In NK cells, ILT2 inhibits the cytolysis
of HLA-Class I-positive target cells. In T cells, ILT2 inhibits both signaling and cellular events that control the activation of T
cells.
CD85j (ILT2)
Clone: HP-F1 Isotype: IgG1 Mouse
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Human CDs
CD85k, also called ILT3, is a member of a family of genes located on human chromosome
19: Immunologulin (Ig)-like Transcripts (ILT), also known as Leucocyte Ig-like Receptors
(LIRs) and Monocyte/Macrophage Ig-like Receptors (MIRs). In contrast to Killer cell Ig-like
Receptors (KIR), ILT/LIR/MIR are preferentially expressed on monocytes, macrophages,
dendritic cells and granulocytes, except ILT2 (CD85j), which is expressed by both myeloid
and lymphoid cells: NK, T cell subsets and all peripheral B cells. All ILT receptors but one
(ILT6) are transmembrane proteins, with 2 or 4 extracellular Ig-SF (Immunoglobulin Super
Family) domains. ILT3 (also known as LIR5, HM18) is a transmembrane protein of 60 Kda,
with 2 extracellular Ig-SF domains, and 3 cytoplasmic Immunoreceptor Tyrosine-based
Inhibitory Motifs (ITIMs). The CD85k molecule is constitutively phosphorylated. It is
selectively expressed by monocytes, macrophages and dendritic cells (DCs). The ligand of
ILT3 is unknown, however ILT3 seems to be involved in one of the inhibitory mechanisms,
ranging from temporary to permanent inactivation, that control homeostasis and
immunological tolerance.
CD85k (ILT3)
Clone: ZM3.8 Isotype: IgG1 Mouse
The ZM3.8 antibody immunoprecipitates a protein of 55 kDa from ILT3-transfected T cells and a protein of 60 kDa from monocytes.
ILT3 is found on peripheral blood mononuclear cells. It is positive only on CD14
+
monocytes, dendritic cells, CD83
+
and CD14
-
HLA-DR
+
cells (peripheral blood DC precursors). ILT3 is negative on CD3
+
T cells, CD16
-
NK cells, CD20
+
B cells, neutrophils and monocyte-
enriched populations. It is positive on DCs derived from monocytes cultured with GM-CSF
+
IL-4 and on macrophages derived from
monocytes cultured with M-CSF. On myelo-monocytic cell lines. it is positive on THP1, negative on U937, Monomac6 and HL-60. On
EBV-B cell lines, positive only on C1R. CD85k is negative on purified fresh B cells, resting or activated with CD40L in the presence of IL
-2, 4 and 10.
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The CD86 antigen (B7-2, B70) is a single-chain trans-membrane glycoprotein, structurally
similar to CD80. Its molecular weight is 80 kDa, under reducing conditions. The
extracellular region is composed of one V-type and one C-type Ig-like domains. There are
8 potential sites for N-glycosylation. The cytoplasmic tail has 3 potential sites for protein
kinase C phosphorylation. CD86 shares with the CD80 molecule the same co-receptors
on T cells, CD28 and CD152 (CTLA-4). CD86 binds to CD152 with a 20-100-fold higher
affinity than to CD28. CD86 is expressed constitutively by interdigitating dendritic cells,
and at lower level by peripheral blood dendritic cells. It is strongly expressed by
monocytes. On lymphocytes, CD86 appears as a B-cell activation antigen. CD86 is
preferentially expressed by memory B cells and germinal center B cells, but not on plasma
cells. Its expression can be up-regulated by activation through surface immunoglobulin,
CD40, MHC class II molecules or by PMA with ionomycin. T cells activated by CD3
ligation have also been reported to express CD86.
CD86
Staining of Daudi cell line.
Clone: HA5.2B7 Isotype: IgG2b Mouse
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Human CDs
The CD89 antigen (FcR) is a transmembrane glycoprotein with an extracellular part composed of two Ig superfamily domains.
Five sites for N-glycosylation are present. The molecular weight of the molecule is 50-75 kDa. The FcR is the receptor for
soluble IgA1 or IgA2. This antigen is restricted to cells of myelomonocytic lineage and its expression begins at the
promyelocyte differentiation stage.
CD89
Clone: A3 Isotype: IgG1 Mouse
In peripheral blood, the A3 antibody is highly reactive with neutrophils, monocytes and eosinophils. CD3
+
T lymphocytes, CD19
+
B
lymphocytes and CD57
+
NK cells are negative for A3 antibody labeling.
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The human CD90 antigen (also known as Thy-1) is the smallest (18 kDa) member of the
immunoglobulin gene superfamily (IgSF). It is a highly glycosylated molecule with a
glycosyl-phosphatidylinositol (GPI) anchor consisting of a single IgSF variable domain. The
CD90 molecule has a broad tissue distribution and is expressed in nervous tissue,
connective tissue and various stromal cell lines. It characterizes a rare subset of human
fetal bone marrow cells, that contains multipotent hematopoietic progenitor activity. This
antigen is expressed on a subset of CD34 positive cells from human bone marrow, cord
blood or fetal liver. It is also expressed on very small subsets of thymocytes and peripheral
T-lymphocytes.
CD90
Jurkat cell line.
Clone: F15-42-1-5 Isotype: IgG1 Mouse
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Clone: Thy1/310 Isotype: IgG1 Mouse
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Human CDs
CD94 is a type II transmembrane glycoprotein of 30 kDa, belonging to the Ca
++
-
dependent (C-type) lectin family. Like NKG2A (or Kp43) on human natural killer (NK) cells,
CD94 associates with one member of the NKG2 family to form disulphide-linked, NK cell
receptor for MHC class I molecules, with a broader specificity than the human killer cell
inhibitory (KIR) / activator (KAR) receptors of the Ig-superfamily. Ligation of CD94 either
potently triggers or inhibits NK cell proliferation and cell-mediated cytotoxicity. Recent
studies strongly indicate that the specificity of CD94/NKG2 receptors is for HLA-E, a non-
classical MHC class I molecule. CD94/NKG2A and CD94/NKG2B heterodimers
constitute inhibitory NK cell receptors, whereas the association of CD94 with NKG2C
corresponds to an activating receptor. The expression of CD94 appears restricted to most
NK cells and to a T lymphocyte subpopulation including a subset of TCR
+
T-cells and
TCR
+
CD8
+
CD56
+
T-cells, mainly V2/V2.
CD94
Staining of HP3B1 Cell line.
Clone: HP-3B1 Isotype: IgG2a Mouse
Ligation of CD94 with the HP-3B1 monoclonal antibody inhibits IL-2-dependent proliferation of NK-cells, and induces apoptosis in a
subset of IL-2-stimulated NK cells.
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The CD95 (Fas or APO-1) antigen is a 40-50 kDa transmembrane glycoprotein. It belongs to the Tumor Necrosis Factor (TNF)
superfamily and contains three cysteine-rich repeats. This cell surface molecule mediates apoptosis (programmed cell death).
CD95 antigen is expressed on a substantial proportion of peripheral blood CD4
+
lymphocytes, CD8
+
lymphocytes and B
lymphocytes but on a minor proportion of NK cells. It is not found on platelets or erythrocytes, but is expressed variably on
granulocytes and monocytes. CD95 is strongly up-regulated on activated T cells, B cells, NK cells and thymocytes. Moreover,
it is widely expressed on cell lines of T, B, NK and myeloid lineages. Most other tissues express CD95.
CD95
Clone: 7C11 Isotype: IgM Mouse
In vitro, the 7C11 antibody induces apoptosis in some CD95-expressing cell lines. Staining pattern: membrane.
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Clone: CH11 Isotype: IgM Mouse
The CH11 antibody in vitro induces apoptosis in some CD95 expressing cell lines. Staining pattern: membrane.
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- Liquid RUO IM1504 0.05 mg Purified
Clone: UB2 Isotype: IgG1 Mouse
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- Liquid RUO IM1739 50 tests PE
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Human CDs
Clone: ZB4 Isotype: IgG1 Mouse
The ZB4 antibody inhibits apoptosis induced by CH11 antibody. Staining pattern: membrane.
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The CD103 antigen was originally described as the human mucosal antigen 1 (HML-1)
and was subsequently proved to be the integrin E subunit. CD103 is composed of a 150
kDa chain associated with a 25 kDa chain by a disulfide bond. It is expressed as a
heterodimer in association with the integrin 7 chain. CD103 is expressed on mucosa-
associated T lymphocytes and activated cells. This human intra-epithelial lymphocyte
marker is expressed in very few resting peripheral blood lymphocytes. E7 binds to E-
cadherin on epithelial cells. Activation and TGF-1 induce CD103 expression.
CD103
Molt 16 cell line.
Clone: 2G5 Isotype: IgG2a Mouse
The 2G5 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. Tissue reactivity: 2G5 reacts with most
intestinal intra-epithelial lymphocytes and with 40% of lamina propria T lymphocytes in the intestine. Staining pattern: membrane.
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The CD105 antigen is a 170-180 kDa RGD-containing molecule which is present on the
cell surface as a disulphide-linked homodimer. The molecule is a co-receptor for TGF1
and 3. It is expressed mainly on endothelial cells, but also strongly in pre-B lymphocytes
and in a subpopulation of monocytes.
CD105
Human Umbilical Vein Endothelial cells
(HUVECs).
Tissue reactivity: the 1G2 antibody reacts with endothelial cells from various tissues and syncytiotrophoblasts. High Endothelial Venules
(HEV) are strongly positive. It recognizes proerythroblasts from bone marrow. Staining pattern: membrane.
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Human CDs
The CD106 antigen, VCAM-1 (Vascular Cell Adhesion Molecule-1), is an adhesion
molecule which binds to integrin 4/1 (VLA-4) and integrin 4/7 on lymphocytes. It is a
110 kDa membrane protein. CD106 antigen is present on activated endothelial cells,
tissue macrophages, dendritic cells and bone marrow fibroblasts. It is involved in the
adhesion of lymphocytes, monocytes and eosinophils to activated endothelium.
CD106
TNF alpha activated HUVEC cells.
Clone: 1G11
Isotype: IgG1 Mouse
The 1G11 antibody inhibits T lymphocyte adhesion to TNF-stimulated endothelial cells. Tissue reactivity: 1G11 recognizes follicular and
interfollicular dendritic cells of the lymph node, and bone marrow stromal cells. Staining pattern: membrane.
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The CD109 antigen is a monomeric glycosyl phosphatidylinositol (GPI)-linked glycoprotein
of 170 kDa that contains several N-linked endoglycosidase H-sensitive hybrid-type
glycans but no O-linked glycan. It has been reported as a novel member of the 2
macroglobulin (2M) / C3, C4, C5 family of thioester-containing proteins. The CD109
antigen is found on vascular endothelial cells, some epithelial cells, activated, but not
resting, T-cells, activated, but not resting, platelets, leukemic megakaryoblasts and a
subset of bone marrow CD34
+
cells. This antigen is not expressed on fresh peripheral
blood lymphocytes (PBL). Poorly differentiated (CD34
+
, TdT
+
, CD7
+
) T-acute leukemias
and rare cases of chronic myeloid leukemia in megakaryoblast crisis express the CD109
antigen. Furthermore, megakaryoblastoid cell lines (MO7e, MOLM-1) are CD109
+
. The
CD109 antigen, strongly expressed on KG1a cell line with 20,000 binding sites per cell,
may represent a very early marker for hematopoietic cells committed to the megakaryocyte
lineage. In contrast, the adult bone marrow CD34
+
CD109
+
subset identifies the most
primitive hematopoietic stem cells capable of long-term culture and lymphoid progenitors
of the B-cell lineage.
CD109
KG1a cell line
Clone: 8A3 Isotype: IgG2a Mouse
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Human CDs
The CD111 antigen is an integral membrane protein belonging to the Immunoglobulin
superfamily that includes four members (CD111, CD112, nectin 3 and CD155). It was
originally named poliovirus receptor related 1 (PRR1). The extracellular portion of the
CD111 molecule comprises three immunoglobulin-like domains, one V-type domain, and
two C2-type domains. CD111 gene is localized to chromosome 11q23-q24 and encodes
at least for two different transmembrane isoforms with identical ectodomain but different
transmembrane and cytoplasmic regions. The CD111 molecule is expressed by about
50% of CD34-positive cells, and most CD33-positive cells in the bone marrow. PRR1 is
also expressed by a sub-population of glycophorin A-positive cells.
CD111 (Nectin 1)
TF1 cell line.
Clone: R1.302.12 Isotype: IgG1 Mouse
The R1.302.12 antibody was submitted to the 6th HLDA (code MA67) and was assigned to CD111 during the HLDA 7.
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The CD112 antigen is an integral membrane protein belonging to the Immunoglobulin
superfamily that includes four members (CD111, CD112, nectin 3 and CD155). It was
originally named poliovirus receptor (PVR) and was later identified as a molecule related to
the CD155 molecule and named poliovirus receptor related 2 (PRR2). The extracellular
portion of the CD112 molecule comprises three immunoglobulin-like domains, one V-type
domain, and two C2-type domains. CD112 gene is localized to chromosome 19q13.2-
q13.4 and encodes for two different transmembrane isoforms with identical ectodomain
but different transmembrane and cytoplasmic regions. The PRR2 molecule expression is
variable. It is expressed on myelomonocytic and megakayocytic lineages ranging from 20%
to 60% of CD34-positive cells in bone marrow.
CD112 (PRR2)
TF1 cell line.
Clone: R2.477.1 Isotype: IgG1 Mouse
The R2.477.1 antibody was submitted to the 6th International HLDA Workshop (code MA39) but has been assigned to CD112 during the
HLDA 7.
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Human CDs
The CD116 antigen, a 75-85 kDa molecule, is the human Granulocyte-Monocyte Colony-
Stimulating Factor (GM-CSF) receptor chain. The CD116 antigen is not expressed by
lymphocytes, but strongly by monocytes and granulocytes as well as their precursors. It is
also present on endothelial cells, fibroblasts and Langerhans cells. CD116 associates with
the common chain (CDw131) of the IL-3 and IL-5 receptors to form a high affinity
receptor for GM-CSF.
CD116 (GM-CSFR)
Clone: SCO6 Isotype: IgG1 Mouse
The SCO6 antibody recognizes an epitope of the GM-CSF receptor chain. It is a high affinity antibody which binds to the membrane
and to the soluble form of GM-CSFR. It immunoprecipitates the molecule. SCO6 antibody does not work in Western blot assays.
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The CD117 antigen is the 145 kDa protooncogene c-kit. It belongs to the class III
receptor tyrosine kinase family. This antigen is the receptor for the stem-cell factor (SCF)
encoded by the steel locus (Steel Locus Factor "SLF"). The CD117 antigen is expressed
on very few normal bone marrow cells. The majority of CD117+ marrow cells co-express
CD34. CD117 is also expressed on mast cells.
CD117 (c-kit)
MO7E cell line.
Clone: 95C3 Isotype: IgG1 Mouse
The 95C3 antibody reacts with BFU-E, CFU-GEMM, CFU-GM and CFU-G. The 95C3 antibody reduces the binding of SCF to its receptor to
about half of the maximum value.
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Human CDs
Clone: 104D2D1 Isotype: IgG1 Mouse
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The CD118 antigen is the subunit of the human leukemia inhibitory factor receptor
(LIFR). It is a 190 kDa type I transmembrane glycoprotein belonging to the hematopoietin
family of receptors. It associates with CD130, the common gp130 -chain shared with the
IL-6 cytokine family. The subunit extracellular domain, composed of 789 residues,
consists of an N-terminal domain followed by two cytokine receptor homology (CRH)
domains, separated by an immunoglobulin-like domain, and three fibronectin type III-like
domains close to the membrane. The two CRH domains contain close to their C-terminal
two conserved tryptophan-serine-X- tryptophan-serine (WSXWS) motifs. The
transmembrane domain of 25 residues precedes the cytoplasmic tail of 239 residues. The
CD118 antigen is not found on circulating blood cells, but it is expressed by a wide variety
of cells including: embryonic stem cells, hepatocytes, adipocytes, neural and glial cells,
osteoblast, and myoblasts. Two ligands are known for the heterodimeric receptor, the LIF
and the OSM cytokines. The LIF cytokine binds to the Ig-like domain of the LIFR chain
with low affinity and then bind to the CHR domain of the -chain to deliver the signal
transduction by activation of two pathways, the JAK/STAT pathway, and the
RAS/RAF1/MEK/ERK MAP kinase pathway. LIF, initially described on the basis of its ability
to induce differentiation of murine myeloid leukemia cells, is a pleiotrophic cytokine that
induces expression of proinflammatory cytokines, and plays important roles in the immune
system, during hematopoiesis, in heart development, in adipocyte lipid transport, in
reproduction, in neural system and in bone remodeling.
CD118 (LIFR)
LIFR-transfected cell line.
The 7G7 monoclonal antibody is a non-blocking monoclonal antibody.
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Human CDs
The CD120a molecule (also known as tumor necrosis factor-receptor type 1, or TNF-R1)
is a 55 kDa, integral membrane glycoprotein. Its extra-membraneous part shows 4
cysteine-rich domains (CRDs), one of which seems to be required for ligand binding.
CD120a is the main receptor for TNF-. Also able to bind to this receptor, although with
lower affinity than TNF-, are lymphotoxin- (LT), also known as TNF- when secreted
as a soluble homotrimer, as well as the membrane-associated LT2/1 heterotrimer. From
a functional perspective, signaling by the TNF receptor family requires clustering of 3
receptors by trimeric ligands and subsequent association of the intracellular tails with
transduction factors. The cytoplasmic tail of CD120a carries a death domain (DD) which
can be involved in an apoptotic cascade. However, this receptor can trigger multiple
potential downstream pathways; apart from being able to mediate apoptosis, it can
modulate inflammation or activate nuclear factor-kappa B (NF-B). Phosphorylation of
CD120a by p42
mapk/erk2
inhibits the apoptotic activity of CD120a while preserving its ability
to activate NF-B. Virtually all nucleated mammalian cells, including lymphocytes,
monocytes, macrophages neutrophils, endothelial cells, hepatocytes, chondrocytes, and
fibroblasts, constitutively express CD120a receptor at low medium levels. In contrast,
basophils, and eosinophils express very low or undetectable levels of CD120a, whereas
mature erythrocytes and platelets are uniformly negative for its expression.
CD120a
Clone: H398 Isotype: IgG2a Mouse
The H398 monoclonal antibody (mAb) blocks the binding of TNF- to the CD120 molecule.
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The CD120b molecule, also known as tumor necrosis factor-receptor 2 (TNF-R2) is a 75
kDa, integral membrane glycoprotein and a member of the tumor necrosis factor (TNF)
receptor superfamily. Its extra-membraneous portion shows 4 cysteine-rich domains
(CRDs), one of which seems to be required for ligand binding. The CD120b molecule is a
receptor for TNF-. Lymphotoxin- (LT), also known as TNF- when secreted as a
soluble homotrimer, as well as the membrane-associated LT2/1 heterotrimer. Soluble
TNF resulting from cleavage of membrane TNF by the TNF- converting enzyme (TACE)
can also bind to the CD120b receptor. From a functional perspective, signaling by TNF
receptor family requires clustering of 3 receptors by trimeric ligands and subsequent
association of the intracellular tails with transduction factors, like the TNF receptor-
associated factors (TRAF). TNF-R2 triggering can lead to NF-B activation, but does not
result in cell death. However, the most effective cell killing occurs when membrane bound
TNF crosslinks both CD120a and CD120b receptors. The CD120b molecule (TNF-R2) is
restricted to hematopoietic cells. It is expressed on monocytes, neutrophils, NK cells and
dendritic cells, as well as on a subset of T cells. Very weak expression can be seen on
some B cells, and no expression is reported on basophils.
CD120b (TNF-R2)
Clone: 80M2 Isotype: IgG1 Mouse
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Human CDs
The CD122 antigen is the human IL-2 receptor chain (p75). CD122 associates with
CD25 and CD132 (IL-2R) to form the IL-2 high affinity receptor. It is also a component of
the IL-15 receptor. CD122 can directly bind IL-2 and is involved in IL-2 signal
transduction. The CD122 antigen is expressed on NK cells, B and T lymphocytes and
monocytes.
CD122 (IL-2R -chain)
lymphocytes.
Clone: CF1 Isotype: IgG1 Mouse
CF1 is a non blocking antibody.
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Clone: 2RB (IL-2R (p75)) Isotype: IgG1 Mouse
The 2RB antibody blocks IL-2 binding.
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The CD123 antigen, alias Interleukin-3 alpha receptor (IL-3R), belongs to the cytokine receptor family. It is constitutively
expressed by committed hematopoietic stem / progenitor cells, by most of the myeloid lineage (CD13
+
, CD14
+
, CD33
+
,
CD15
low
), and by some CD19
+
cells, it is absent from CD3
+
cells.
CD123 (IL-3R)
Clone: SSDCLY107D2 Isotype: IgG1 Mouse
The SSDCLY107D2 monoclonal antibody reacts with plasmacytoid dendritic cells in situ (PDCs).
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Human CDs
The CD124 antigen (140 kDa) is the human IL-4 receptor chain. The CD124 antigen
associates with CD132 (IL-2R) to form the high affinity IL-4 receptor or with IL-13R
chain to form the high affinity IL-13 receptor. CD124 expression on resting lymphocytes is
upregulated following cell activation. The CD124 antigen is expressed by mature B and T
cells, hemopoietic precursors, fibroblasts and endothelial cells.
CD124 (IL-4R)
lymphocytes.
Clone: S456C9 Isotype: IgG1 Mouse
The S456C9 antibody recognizes the membrane and soluble form of IL-4R. It is a blocking antibody.
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The CD126 antigen is the human IL-6 receptor chain. It is an integral membrane protein
of 80 kDa. In peripheral blood it is detectable on monocytes, on activated B lymphocytes,
and on CD4
+
T lymphocytes. This antigen is also expressed by epithelial cells. The CD126
antigen associates with CD130 to form a high affinity IL-6 receptor. A soluble form of
CD126 (sIL-6R) can be detected in plasma.
CD126 (IL-6R)
lymphocytes.
Clone: M91 Isotype: IgG1 Mouse
The M91 antibody recognizes the membrane and soluble form of IL-6R chain. It binds to the amino terminal immunoglobulin domain
of IL-6R. It binds to IL-6/IL-6R complexes and IL-6/IL-6R/gp130 complexes. It is a non-blocking antibody suitable for Western blot
assays.
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Human CDs
The CD127 antigen is the human IL-7 receptor chain. It is a 75-80 kDa molecule.
CD127 is found on normal B cell precursors but not on mature B lymphocytes. It is also
expressed by the majority of T lymphocytes, a subset of monocytes and a subset of CD34
+
cells. CD127 associates with CD132 (IL-2R) to form the high affinity IL-7 receptor.
Receptor expression is down-regulated following T cell activation.
CD127 (IL-7R)
lymphocytes.
Clone: R34.34 Isotype: IgG1 Mouse
The R34.34 antibody inhibits binding of IL-7 to CD127.
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Human CDs
The CD130 antigen, also known as gp130 transducer chain, is a type 1 transmembrane
glycoprotein, containing 10 potential N-linked glycosylation sites on its extracellular region,
which is composed of six fibronectin type 3 (F3) modules. The second and third F3
modules form a class 1 cytokine receptor domain. The high affinity IL-6 receptor is built by
non-covalent association of CD130 and CD126 (IL-6R); when IL-6 is first engaged by
CD126 and then presented to CD130, two heterotrimeric complexes (IL
-6/CD126/CD130) pool together to form a functional signaling competent hexamer.
Several other cytokines, including IL-11, leukemia inhibitory factor (LIF), ciliary neurotrophic
factor (CNTF), cardiotrophin-1 (CT-1) and oncostatin M (OSM) can interact with gp130.
Binding of LIF, CNTF, CT-1, or OSM leads to heterotrimerization of the cytokine/gp130
complex with a unique third protein, the LIF Receptor (LIF-R). The IL-6-type cytokines play
important roles in receptor-carrying cell activation, proliferation and differentiation. IL-6-
type cytokines activate through the gp130 cytoplasmic domain the Janus kinases (Jak)
/ signal transducers and activators of transcription (STAT) pathway as well as the mitogen-
activated protein kinase (MAPK) cascade. Various mechanisms are also involved in the
termination of the IL-6-type cytokine signaling; both the suppressor of cytokine-signaling
SOCS3, and the protein-tyrosine phosphatase SHP2 can be recruited to the same
intracellular site (tyrosine 759 residue) of the gp130 transducer chain. Studies suggest
that there are two, largely distinct modes of negative regulation of gp130 activity. The
CD130 molecule is expressed at low levels on almost all cell types, including a subset of
CD4
+
T lymphocytes, most monocytes, basophils, endothelial cells, epithelial cells,
fibroblasts, hepatocytes and neural cells. It is expressed at high levels on activated and
EBV-transformed B cells. It is not expressed on resting B cells, NK cells, nor is it
expressed on neutrophils or dendritic cells.
CD130 (IL-6R)
Clone: TR5.7.5 Isotype: IgG1 Mouse
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Human CDs
The CD134 antigen is a 35 kDa, type-1 transmembrane glycoprotein member of the tumor
necrosis factor superfamily (TNFRSF) and is allocated number 4. This antigen was
originally named OX40 from the first antibody that led to the discovery of this antigen on
activated rat CD4
+
T cells. In humans, CD134 is expressed at high levels on activated
CD4
+
T cells and virally stimulated CD4
+
T cells. Its ligand, known as gp34, is a type-2
transmembrane glycoprotein member of the tumor necrosis factor (TNF) superfamily,
expressed on activated T and B cells, dendritic cells and macrophages. The CD134
antigen, through its interaction with adaptor proteins TRAF2 and TRAF5, is a costimulatory
signal transducer of T-cell receptor-mediated activation that has been shown to activate
NF-B, a signaling factor involved in transcription of survival genes. Thus, CD134
engagement with its ligand induces clonal expansion and survival of CD4
+
cells during
primary responses, and results in the accumulation of increasing numbers of memory cells
with time. Finally, CD134 has been shown to be involved in the T cell adhesion to
endothelium.
CD134 (OX 40)
Clone: Ber-ACT35 Isotype: IgG1 Mouse
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CD135, also called FLT3 or FLK2, is an integral membrane protein of 130 kDa to 150 kDa, which belongs to the receptor
tyrosine kinase (RTK) family. These receptors directly bind extracellular ligands and transduce regulatory signals through
intracytoplasmic tyrosine kinase activity. The CD135 antigen is expressed on myelomonocytic and primitive B cell progenitors.
On normal bone marrow cells, expression of CD135 can be found on fractions of CD34
+
as well as CD34
-
cells. Most of the
CD34
+
CD135
+
cells co-express c-kit (CD117) at high levels.
CD135 (flt3/flk2)
Clone: SF1.340 Isotype: IgG1 Mouse
The SF1.340 antibody is specific for an extracellular domain of CD135. This antibody does not compete with the FLT3 ligand for binding
to FLT3.
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Human CDs
The CD136 antigen is a heterodimeric protein consisting of an chain (40 kDa) and a
chain (150 kDa) linked by a disulfide bond, and originated by proteolytic cleavage of a
single-chain precursor of 185 kDa. The chain is a type I integral membrane protein (with
an extra-cellular region, a transmembrane segment, and an intracellular tyrosine kinase
domain). The chain is exclusively extracellular. Also known as macrophage-stimulating
protein receptor (MSP receptor), the CD136 antigen is a member of the scatter factor
receptor family. Scatter factors control a complex genetic program involving cell survival
and motility programs related to invasive growth. The CD136 antigen is expressed on
resident macrophages, epithelial cells, keratinocytes, and erythroblasts. MSP-induced
activation of CD136 results in trans-phosphorylation of MET, another scatter factor. The
MSP-CD136 signaling pathway has been implicated in the functional regulation of
mononuclear phagocytes. In erythropoiesis, CD136 signaling contributes to erythroblast
renewal but not to their differentiation.
CD136 (RON)
MDCK-RE7, CD136 transfected cell line
Clone: ID1 Isotype: IgG2a Mouse
The ID1 monoclonal antibody blocks ligand binding. It has been clustered as CDw136 (WS Code: C-1) during HLDA 6.
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The CD138 antigen (also known as syndecan-1) is a member of the syndecan family, which is comprised of 4 types of
transmembrane heparan sulfate proteoglycans macromolecules (syndecan-1, -2 or fibroglycan, -3 or N-syndecan, and -4 or
amphiglycan). Syndecan molecules are involved in interactions with extracellular matrix proteins, with cell-surface molecules
and with soluble proteins such as cytokines. Most adherent mammalian cells are reported to express heparan sulfate
proteoglycans (including syndecan-1) and the expression seems to be developmentally regulated.
CD138
Clone: B-A38 Isotype: IgG1 Mouse
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Human CDs
CD144, also called Cadherin 5 or VE-Cadherin is a 140 kDa protein belonging to the
cadherin family of cell adhesion molecules. CD144 antigen is specific for endothelial cells
and is located at the intercellular cleft sites of the junction within endothelial tissue. The
cadherin 5 molecule may play a role in the permeability properties of vascular endothelium.
CD144 (VE-Cadherin)
Lysed whole blood sample.
Clone: TEA 1/31 Isotype: IgG1 Mouse
Tissue reactivity: on frozen tissue sections, TEA1.31 stains intercellular cleft sites of the junction between endothelial cells. Staining
pattern: membrane.
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The CD146 molecule, also known as S-Endo, is a single chain transmembrane glycoprotein with a molecular weight of 118
kDa, belonging to the immunoglobulin superfamily (IgS). The extracellular structure consists of five Ig-like domains: two V-type,
and three C2-type Ig-like domains. The CD146 antigen is related to cell adhesion molecules of the IgS family and its presence
is concentrated at regions of cell-cell contact, confirming its role in adhesion and particularly in maintenance of monolayer
integrity. CD146 is constitutively expressed in the endothelium, and is also observed on other cell types such as smooth
muscle cells, and intermediate trophoblasts. Among peripheral whole blood leucocytes, CD146 expression is not detected,
except on a subset of activated T-lymphocytes.
CD146
Clone: TEA 1/34 Isotype: IgG2a Mouse
The TEA 1/34 monoclonal antibody was assigned to the CD146 (Code: E077) during the HLDA 6.
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Human CDs
The CD152 antigen, also called CTLA4, is an integral membrane protein belonging to the
immunoglobulin superfamily, which is expressed either as a 30 kDa monomer or as a
disulfide-linked homodimer. It has homology with the T cell antigen CD28, and like CD28,
is a ligand for CD80 and CD86. CTLA-4 is transiently expressed by activated T cells and
is not detectable in resting T cells. CD152 and CD28 are involved in the costimulation
required for T cell activation.
CD152 (CTLA-4)
Activated peripheral blood lymphocytes
(PBL).
Clone: BNI3 Isotype: IgG2a Mouse
The BNI3 antibody can be used to detect intracytoplasmic CD152 (CTLA4) by flow cytometry after permeabilization with IntraPrep
Permeabilization Reagent.
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The CD154 antigen (CD40-ligand), also known as TRAP (Tumor necrosis factor Related
Activation Protein), is a member of the Tumor Necrosis Factor (TNF) superfamily. It is a 32
kDa type II transmembrane molecule. CD154 molecule is induced on the surface of
activated T cells, mainly on CD4
+
T lymphocytes. This ligand for the CD40 molecule is
present on some basophils and mast cells. It has critical regulatory roles in T- and B-
lymphocyte function.
CD154
3XTBA7 cell line.
Clone: TRAP-1 Isotype: IgG1 Mouse
The TRAP-1 antibody inhibits activation induced by full-length soluble human CD154.
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Human CDs
CD155 (Poliovirus Receptor; PVR) is a transmembrane glycoprotein of 80-90 kDa, with 3 extracellular immunoglobulin-related
regions. Four different isotypes ( to ) exist, originating from alternate splicing of the CD155 mRNA. CD155 and are
secreted, whereas CD155 and are membrane-anchored and serve as the PVR, via the V-region of the N-terminus. CD155
is expressed on monocytes, a T-cell subset and neurons of the central nervous system. In monocytes, a physical interaction of
CD155 with CD44 has been shown, suggesting a role in the regulation of CD44 ligand binding. CD155 expression is
restricted to myeloid cells, including committed progenitor cells, and a T cell subset; in non-hematopoietic cells, CD155
expression is restricted to the neurons of the central nervous system.
CD155
Clone: PV404.19 Isotype: IgG1 Mouse
The PV.404 monoclonal antibody immunoprecipitates a protein of 80-90 kDa isolated from Ficoll-separated peripheral blood
mononuclear cells. It also blocks the binding of poliovirus entry into the monocytes.
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The CD157 antigen (BST-1) is a highly glycosylated, glycosyl-phosphatidylinositol (GPI)-anchored membrane protein with a
molecular weight of 43 kDa. The amino acid sequence of human BST-1 has 33% identity with CD38. As with CD38, BST-1
displays ADP ribosyl cyclase activity and facilitates pre-B cell growth. CD157 antigen is expressed on monocytes, neutrophils
and all lymphoid and myeloid precursors. Its expression is restricted to certain fractions (CD44
-
CD25
+
and CD44
-
CD25
-
) of
CD3
-
CD4
-
CD8
-
T progenitors.
CD157
Clone: RF3 Isotype: IgG Mouse
The RF3 antibody reacts with an external epitope of BST-1 on several human cell lines including certain bone marrow stromal cell,
human umbilical vein endothelial cell (HUVEC) and U937 cell lines.
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173
Human CDs
The CD158a and CD158h molecules are monomeric integral membrane glycoproteins
existing in different forms. CD158a, or p58.1, is a 58 kDa molecule, and CD158h, or
p50.1, is a 50 kDa molecule, both having identical extracellular portions, but different
transmembrane and cytoplasmic regions. Another comprehensive nomenclature refers to
these molecules as KIR2DL1 for CD158a and KIR2DS1 for CD158h. These acronyms
stand for Killer cell Immunoglobulin (Ig)-like Receptor (KIR), with extracellular part
containing 2 Ig Domains (2D), and cytoplasmic tails being either long (L) or small (S). The
long intracellular portion of CD158a, or p58.1, makes it an inhibitory form, since it contains
two characteristic ITIM motifs (Immunoreceptor tyrosine based inhibition motif) and is
involved in the transduction of the inhibitory signal. Whereas the stimulatory form CD158h,
or p50.1, lacks these ITIM motifs. CD158a/p58.1 functions as an inhibitory receptor for a
group of HLA-C molecules (Cw2, cw4, Cw5 and Cw6 supertypes). The inhibitory effect is
dominant over the activation exerted by the activator form p50.1. The CD158a and
CD158h molecules are indistinguishable from the extracellular viewpoint. They are
expressed by a subset of NK cells, and can be detected on the surface of sub-populations
of peripheral T lymphocytes, mostly of the CD8
+
phenotype.
CD158a,h
Clone: EB6B Isotype: IgG1 Mouse
The EB6B antibody reacts with both CD158a (p58.1 or KIR2DL1) and CD158h (p50.1 or KIR2DS1). The antibody restores the ability of
p58.1
+
NK clones to lyse targets expressing HLA-C molecules.
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Human CDs
The CD158b1, CD158b2 and CD158 j molecules are monomeric integral membrane glycoproteins existing in different forms.
CD158b1 and CD158b2 (p58.2 and p58.3, respectively) are 58 kDa molecules. CD158j (p50.2) is a 50 kDa molecule. All
three have identical extracellular portions, but different transmembrane and cytoplasmic regions. CD158b1 and CD158b2
likely represent allelic forms of the same locus. There is another comprehensive nomenclature which refers to these molecules
as KIR2DL2 for CD158b1 (p58.2), KIR2DL3 for CD158b2 (p58.3), and KIR2DS2 for CD158j; these acronyms stand for Killer
cell Immunoglobulin (Ig)-like Receptor (KIR), with extracellular part containing 2 Ig Domains (2D), and cytoplasmic tails being
either long (L) or short (S). The long intracellular portion of CD158b1/b2 (p58.2/p58.3) makes them inhibitory forms, since
they contain two characteristic ITIM motifs (Immunoreceptor Tyrosine-based Inhibition Motif) and are involved in the
transduction of an inhibitory signal through the activation of the protein tyrosine phosphatases SHP-1 and/or SHP-2. The
stimulatory form CD158h (p50.1) lacks these ITIM motifs. CD158b1/b2 (p58.2/p58.3) function as inhibitory receptors for a
group of HLA-C molecules (Cw1, Cw3, Cw7 and Cw8 supertypes). The inhibitory effect is dominant over the activation
exerted by the activator form p50.2. The CD158b1/b2 and CD158j molecules are indistinguishable from the extracellular
viewpoint. They are expressed by a subset of NK cells, and can be detected on the surface of sub-populations of peripheral T
lymphocytes, mostly of CD8
+
phenotype.
CD158b1/b2,j
Clone: GL183 Isotype: IgG1 Mouse
The GL183 antibody reacts with both p58.2/p58.3 and p50.2. The antibody restores the ability of p58.2
+
NK clones to lyse targets
expressing HLA-C molecules.
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Human CDs
The CD158e1 and CD158e2 molecules (also known as p70 molecules) are monomeric
integral membrane glycoproteins of 70 kDa. CD158e1 and CD158e2 likely represent
allelic forms of the same locus of the NKB1 gene. These proteins belong to the family of
NK receptors for MHC class I molecules and show a high degree of homology with p58
and p140 receptors. There is another comprehensive nomenclature which refers to these
molecules as KIR3DL1 for CD158e1, and KIR3DS1 for CD158e2. These acronyms stand
for Killer cell Immunoglobulin (Ig)-like Receptor (KIR), with extracellular part containing 3 Ig
Domains (3D), and cytoplasmic tails being either long (L) or short (S). CD158e1
comprises 3 C-type extracellular Ig domains, and two characteristic ITIM motifs
(Immunoreceptor Tyrosine-based Inhibition Motif) in the intracellular portion. These motifs
are involved in the transduction of an inhibitory signal through the activation of the protein
tyrosine phosphatases SHP-1 and/or SHP-2. CD158e1/e2 function as inhibitory
receptors for HLA-B molecules of the Bw4 supertype. These molecules are expressed by
a subset of NK cells, and can be detected on the surface of sub-populations of peripheral
T cells, mostly of CD8
+
phenotype.
CD158e1/e2
lymphocytes.
Clone: Z27.3.7 Isotype: IgG1 Mouse
The anti-KIR/p70 antibody Z27 restores the ability of p70+ NK clones to lyse HLA-Bw4 targets.
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The CD158i molecule (also known as p50.3) is a monomeric integral membrane
glycoprotein of 50 kDa. This protein belongs to the family of NK receptors for MHC class I
molecules. There is another comprehensive nomenclature which refers to this molecule as
KIR2DS4; this acronym stands for Killer cell Immunoglobulin (Ig)-like Receptor (KIR), with
extracellular part containing 2 Ig domains (2D), and a short (S) cytoplasmic tail. CD158i
comprises 2 C-type extracellular Ig domains. The intracytoplasmic portion lacks
characteristic ITIM motifs (Immunoreceptor Tyrosine-based Inhibition Motif) and displays a
charged residue in the trans-membrane portion. CD158i receptor seems involved in NK
cell activation, following HLA-Cw3 supertype recognition. Antibody-induced cross-linking
of CD158i (p50.3) results in both early activation events and late effector function.
CD158i
CD3- lymphocytes.
Clone: FES172 Isotype: IgG2a Mouse
The FES172 antibody stains NK subsets, but the p50.3 antigen appears to be expressed only by some individuals, and to be co-
expressed with p58 KIRs.
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Human CDs
CD159a (NKG2a) is a 40 kDa (reduced), 75 kDa (unreduced) type II transmembrane
protein associated with CD94 to form a disulphide-linked heterodimer. CD159a is a
member of the Ca
++
-dependent (C-type) lectin family. The CD94/NKG2A dimer is an
inhibitory NK receptor for MHC Class I, with a broader specificity than Killer cell
Immunoglobin (Ig)-like Receptors (KIR). The intracellular portion of CD159a (NKG2A)
contains two characteristic ITIM motifs (Immunoreceptor tyrosine based inhibition motif)
involved in the transduction of the inhibitory signal. The CD159a (NKG2A) antigen is
always co-expressed with CD94. The CD94/NKG2A complex is detectable in sub-
populations of NK cells, T cells and thymocytes.
CD159a (NKG2a)
lymphocytes.
Clone: Z199 Isotype: IgG2b Mouse
The Z199 antibody reacts specifically with CD94
bright
NK cells expressing the inhibitory form of the CD94 molecules. It does not stain the
activator form of CD94 which corresponds to the association of CD94 with other products of the NKG2a gene family. The antibody
restores the ability of CD94
bright
NK clones to lyse HLA class I targets.
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The CD160 molecule (BY55 antigen) is a 27-kDa, glycosyl phosphatidylinositol (GPI)-anchored glycoprotein of 181 amino
acids, with a single immunoglobulin (Ig)-like domain. The molecule contains two potential sites for N-glycosylation and is rich in
cysteine (6 residues in the mature polypeptide), indicating an important capacity for forming intra- and inter-chain disulfide
bonds, which likely support the disulfide-linked multimeric (80 kDa) expression at the cell surface. Its expression is highly
restricted to circulating cytotoxic T and NK cells, and in tissues, to intestinal intraepithelial lymphocytes, as revealed by CD160
mRNA blot analysis and various phenotyping studies. In peripheral blood, CD160
+
cells consist of a majority of CD3
TCR
+
NK cells, which is significantly reduced in HIV
+
individuals, and of a minority of CD3
+
CD8
bright
T lymphocytes, which is
significantly increased in HIV
+
individuals. In bone marrow, CD160
+
cells, though less abundant than in adult peripheral blood,
also consist of the two populations observed in peripheral blood. In cord blood, CD160
+
cells consist of a unique CD3
TCR
NK cell population, 80% of these cells being CD56

+
. CD160 is a ligand for classical and non-classical MHC-class I
molecules. The ligation of MHC-class I by CD160 provides a costimulatory signal restricted to peripheral blood activated T
cells, suggesting a pathway being additional and/or alternative to the CD28 costimulation mechanism. This pathway may be
relevant in memory T cells that lack CD28, such as the intestinal intraepithelial lymphocytes.
CD160
Clone: BY55 Isotype: IgM Mouse
The BY55 antibody was initially reported to react with a protein structure of 80 kDa exclusively expressed by circulating cytotoxic
lymphocytes. It does not block the binding of the CD160 molecule to MHC-class I molecules.
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Human CDs
The CD161 antigen is also designated as the NKRP1 molecule. CD161 is a type II integral membrane protein expressed as a
monomer of 44 kDa or as a disulphide-linked homodimer of 80 kDa. The carboxy-terminal extracellular domain is homologous
to Ca
++
-dependent (C-type) lectin superfamily. CD161 is an activatory NK receptor, but in contrast with other NK receptors
such as KIR (p58, p70, p140) and CD94/NKG2, CD161-mediated activation is not HLA-dependent. CD161 is expressed on
all NK cells and on sub-populations of T lymphocytes.
CD161 (NKR-P1)
Clone: 191B8 Isotype: IgG2a Mouse
The 191B8 antibody partially blocks the spontaneous NK cytotoxicity in the P815 model.
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P-selectin glycoprotein ligand 1 (PSGL-1), or CD162 antigen, was identified as a ligand for CD62P (P-selectin). It is a type I
integral membrane protein that is thought to be expressed as a 220 kDa disulfide-linked homodimer. This molecule belongs to
the sialomucin family. CD162 is expressed on most T lymphocytes as well as peripheral monocytes and neutrophils. A
subpopulation of B lymphocytes and some CD34
+
bone marrow cells also express CD162. Studies indicate that the CD162
molecule displays Cutaneous Lymphocyte-associated Antigen (CLA).
CD162
Clone: 3E2-25-5 (PL1) Isotype: IgG1 Mouse
The epitope recognized by the 3E2-25-5 (PL1) antibody on PSGL-1 is in close proximity to the P-selectin binding site and the antibody
completely inhibits the binding of P-selectin to PSGL-1 in a variety of assays. It can be used in flow cytometry and in
immunohistochemistry on frozen and paraffin embedded tissue sections.
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Clone: 5D8-8-12 (PL2) Isotype: IgG1 Mouse
The 5D8-8-12 (PL2) antibody does not block the binding of P-selectin to PSGL-1. It can be used in flow cytometry and in
immunohistochemistry on frozen and paraffin embedded tissue sections.
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CD162R antibodies recognize an NK cell-restricted epitope that is brightly expressed on the functionally differentiated CD56
dim
NK cells and at very low density on about one third of CD56
bright
NK cells. The CD162R antigen, also known as PEN5, is
present on the majority of CD56
+
and CD16
+
peripheral blood lymphocytes, and is absent from CD3
+
T cells and CD20
+
B
cells. Neither T- nor B-cell activation induces the cell surface expression of the PEN5 epitope. The CD162R antigen is an
unusual sulfated poly-N-lactosamine carbohydrate that can be expressed on keratan sulfate glycosamino-glycans.
CD162R (PEN5)
Clone: 5H10.21.5 Isotype: IgM Mouse
Immuno precipitates prepared using the 5H10 antibody reveal two polydisperse membrane-bound glycoproteins, PEN5 (120-170 kDa)
and PEN5 (210-245 kDa).
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Human CDs
The CD166 antigen, also known as activated leukocyte cell adhesion molecule (ALCAM),
is a single chain type 1 transmembrane glycoprotein, containing 10 potential N-linked
glycosylation sites, and belonging to the immunoglobulin superfamily (IgSF). It shows a
100 105 kDa molecular weight, and is composed of five extracytoplasmic Ig-like
domains linked to a 32-amino acid cytoplasmic tail through a short membrane-spanning
region. The CD166 antigen is an adhesion molecule that binds to the CD6 molecule, or
forms ALCAM-ALCAM homophilic interactions. Studies suggest that CD166 molecules
can oligomerize at the site of cell-cell contact via their three C2-type Ig-like proximal
domains. The CD166 molecule appears to be a non-lineage restricted molecule,
expressed on a variety of cell types, including selected hematopoietic cells, endothelial
cells, cortical and medullar thymic epithelial cells, bone marrow mesenchymal stem cells,
fibroblasts, and hepatocytes. In peripheral blood, ALCAM is expressed on activated T- and
B-cell subsets, on monocytes, on circulating dendritic cells, and weakly on granulocytes.
While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of
cells involved in dynamic growth and/or migration. In the thymus, the interaction between
the CD6 molecule, as expressed on CD6
+
thymocytes, and the CD166 molecule
expressed on thymic epithelial cells may play a role in T cell development. Other studies
suggest that the CD166 adhesion molecule is involved in embryonic hematopoiesis, as
well as in hemangioblast differentiation and capillary tube formation.
CD166 (ALCAM)
Clone: 3A6 Isotype: IgG1 Mouse
The 3A6 antibody blocks the binding of the CD6 ligand to its CD166 receptor as seen on adhesion experiments of CD6-expressing COS
cells to thymic epithelial cells. The 3A6 antibody was clustered to the CD166 during HLDA 6.
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The CD178 antigen, or Fas ligand (FasL) is a type II membrane protein of 40 kDa and is a member of the tumor necrosis factor
(TNF) family. It is expressed by activated T cells and NK cells and in a very restricted number of tissues known to be "immune
privilege" tissues. Binding of FasL to its receptor Fas/CD95 induces apoptosis. A 26-kDa soluble form of human FasL (sFasL)
has been detected in the supernatant of human peripheral NK lymphocytes upon activation.
CD178 (Fas L)
Clone: 4A5 Isotype: IgG Hamster
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Human CDs
The CD179a molecule (Vpre-B) non-covalently associates with CD179b (also known as
5 or -like) to form an immunoglobulin (Ig) light chain-like structure, known as the
surrogate light chain. The CD179a / CD179b surrogate light chain is disulfide-linked to
the membrane-bound Ig heavy chain in association with the transducer CD79a /
CD79b heterodimer. The CD179a / CD179b / CD79a / CD79b complex then forms a B
cell receptor-like structure known as pre-B Cell Receptor (preBCR). B cell development
from pluripotent stem cells proceeds through pro-B, pre-B and B stages, characterized by
sequential Ig gene rearrangements and transcription, accompanied by the expression of a
well-defined set of cell surface molecules. PreBCR may function as a checkpoint in early B
cell development and may monitor the production of the Ig heavy chain through a
functional rearrangement of the Ig heavy chain gene. Furthermore, preBCR may serve to
check Ig heavy chain association with the Ig light chain. CD179a is not detected in
normal mature circulating B lymphocytes and leucocyte subsets. Low levels of CD179a
can be detected on the cell surface of early pre B cells but the vast majority of CD179a
proteins are retained in the cytoplasm.
CD179a (Vpre-B)
Human pre-B NALM6 cell line.
The 4G7 monoclonal antibody (mAb) recognizes the VpreB protein regardless of whether it is or it is not associated with the chain.
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Human CDs
CD184 (also known as CXCR4, or Fusin) is a seven-transmembrane domain G protein-
coupled surface receptor. CD184 (CXCR4) natural ligand is the stromal cell-derived
factor-1 (SDF-1), a C-X-C chemokine (the nomenclature specifies that this chemokine has
a single amino acid between the first two cysteines of the characteristic four cysteine
motif), which is strongly chemotactic for monocytes and lymphocytes. But CD184
(CXCR4) has also initially been described as the human immunodeficiency virus-1 (HIV-1)
entry co-receptor, associated with the surface CD4-gp120 complex before HIV-1 enters
target cells. It also acts as an alternative receptor for some isolates of HIV-2. The SDF
-1 / CXCR4 pair may also play a role in the development of the nervous system. Unlike
other chemokine receptors, the cellular distribution of CD184 (CXCR4) is nearly
ubiquitous; it is expressed on a variety of CD4
+
and CD4
cells in the immune system and

the central nervous system. In blood, CD184 (CXCR4) is highly expressed on neutrophils,
monocytes, dendritic cells, NK cells, B cells, and T cells, as well as on platelets. CD184
(CXCR4) exhibits a rapid (30 seconds) up-regulation upon activation of lymphocytes,
followed by internalization (within 2 minutes). Accordingly, CD184 (CXCR4) is detected in
large amounts in intracellular stores within leucocytes. Absent from eosinophils and
basophils in fresh whole blood, CD184 (CXCR4) is inducible ex vivo on eosinophils by
glucocorticoids, and on basophils by SDF-1. As a GPCR, CXCR4 binding of SDF-1
triggers certain signaling pathways such as ras, and PI3 kinase. One downstream effect of
SDF-1 / CXCR4 interaction is the activation of transcription factors such as AP-1 and
chemokine regulated genes. JAK / STAT pathways also play a role in SDF-1/CXCR4
signaling.
CD184 (CXCR4)
Clone: 12G5 Isotype: IgG2a Mouse
The 12G5 antibody partially inhibits chemotaxis and calcium influx induced by SDF-1. It stains predominantly the nave, resting CD26
low
CD45RA
+
CD45R0
-
T lymphocyte subset of peripheral blood T cells. The 12G5 mAb has been assigned to the CD184 cluster of
differentiation during HLDA 7.
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Human CDs
The CD203c molecule is a type II transmembrane protein that belongs to the
ectonucleotide pyrophosphatase / phosphosdiesterase 3 (E-NPP3) family of enzymes
involved in hydrolysis of oligonucleotides, nucleoside phosphates, and NAD.
CD203c
CD3 negative/CRTH2 positive leukocytes.
The 97A6 antibody immunoprecipitates two proteins of 270 and 150 kDa at reducing conditions, and one protein of 270 kDa at
nonreducing conditions, suggesting that the 97A6 antigen is a disulfide-linked dimer of two proteins of about 150 kDa each. Basophils
and mast cells are hematopoietic effector cells involved in allergic and inflammatory reactions. Both cell types highly express the high
affinity IgE receptor. The 97A6 antibody recognizes a surface antigen expressed on human peripheral blood basophils, but not on other
blood cells. It also reacts with mature mast cells, and with CD34
+
bone marrow progenitors of basophils and mast cells. Moreover, the
97A6 antigen is up-regulated after activation of basophils by anti-IgE antibodies and allergens.
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CD206, also named macrophage mannose receptor (MMR), is a type I single-chain
transmembrane glycoprotein of 175 kDa with a multilectin receptor structure. The
Mannose Receptor is thought to play a key role in the internalization of sugar-containing
proteins thus concentrating antigens for transport to processing compartments and
peptide loading compartments. First detected on tissue macrophages, the Mannose
Receptor is mainly expressed on immature dendritic cells and on a variety of other cells.
Absent on lymphocytes and monocytes, the Mannose Receptor is up-regulated on
monocytes during their differentiation to macrophages.
CD206 (MMR)
Dendritic cells from in vitro activated
density-purified whole blood sample (PBL).
Clone: 3.29B1.10 Isotype: IgG1 Mouse
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Human CDs
CD207 (Langerin) is an antigen described as selectively expressed by Langerhans cells
(LC), a subset of immature dendritic cells (DC) formed in epidermis and mucosa. Langerin
is a 40 kDa glycoprotein predominantly N-glycosylated which contains no disulfide bond.
Its expression, restricted to the Langerhans cell lineage, is demonstrated at both the
surface membrane and in the cytoplasm. Several lines of evidence indicate that Langerin is
a marker specific for the acquisition of the Langerhans cell phenotype. It is down-regulated
beyond the LC maturation stage. Langerin is not involved in antigen delivery into the MHC
class II pathway but may play a role in antigen capture. Langerin is present only in
epidermis and airway epithelia. Langerin is not detected in purified DCs isolated from
peripheral blood, lymph nodes or thymus. CD1a
+
DCs derived from CD34
+
cord blood cell
cultures in the presence of GM-CSF and TNF- express Langerin between day 8 and 12.
DCs derived from peripheral blood monocytes cultured with GM-CSF and IL-4 do not
express Langerin.
CD207 (Langerin)
Frozen skin, DAB.
Clone: DCGM4 Isotype: IgG1 Mouse
The DCGM4 antibody stains exclusively immature DCs of the LC lineage. Both intracytoplasmic and surface membrane staining is
observed. DCGM4 acts as a ligand for Langerin, triggering rapid endocytosis within 20 minutes at 37
o
C. The antibody has been used in
intracellular immunofluorescence analyzed in confocal microscopy. The DCGM4 antibody has been used to determine the differentiation
of the CD1a
+
/CD11c
+
subset of blood DCs into LCs, and in the characterization of DCs infiltrating breast carcinoma tissue. Tissue
reactivity: skin, tonsil and lung epithelia. Staining pattern: membrane and cytoplasm.
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The DC-LAMP antigen (70-90 kDa) is a new member of the LAMP (lysosome-associated
membrane protein) family. As other LAMP members such as CD68, DC-LAMP is a type I
integral membrane protein of 416 amino acid residues, highly glycosylated (7 N- and some
O- glycosylation sites), with 90% of the molecule located in the lumen of lysosomes. DC-
LAMP is specifically expressed by those mature dendritic cells (DC) located in T cell areas
of lymphoid tissues, which are known as interdigitating dendritic cells (IDC). A direct
correlation between the expression of DC-LAMP and that of other maturation markers
such as CD86 has been established. DC-LAMP first appears in the MHC Class II
compartment, suggesting an important contribution of DC-LAMP to the processing of
exogenous antigens.
CD208 (DC-LAMP)
Fixed tonsil, DAB.
Clone: 104.G4 Isotype: IgG1 Mouse
In flow cytometry, the 104.G4 antibody shows intracytoplasmic staining of DCs derived either from CD34
+
cell cultures activated with
TNF and CD40L, or from monocyte cultures activated with TNF, LPS and CD40L. Tissue reactivity: the 104.G4 antibody exclusively
stains IDCs in lymphoid tissues, as demonstrated with immunohistochemistry. The antibody has been also used in intracellular
immunofluorescence analyzed in confocal microscopy, and in the characterization of DC infiltrating breast carcinoma tissue. Staining
pattern: cytoplasm.
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Human CDs
The CD209 antigen, also named dendritic cell (DC)-specific-intracellular adhesion
molecule 3 (ICAM-3)-grabbing non-integrin (DC-SIGN), is a 44 kDa type II
transmembrane C-type lectin with a single carbohydrate recognition domain (CRD). Like
other C-type lectin members, CD209's extracellular structure is able to bind mannose
residues in a Ca
++
dependent manner. Highly specific to DCs, CD209 plays a key role in
the primary immune response as receptor / processor for a variety of ligands, including:
ICAM-3: initiation of DC-T cell immunological synapse; ICAM-2: transendothelial DC
migration; HIV-1 gp120: capture of HIV-1 and transpresentation to CD4
+
T cells; CMV
envelope glycoprotein B: capture of CMV; Unknown ligands: capture of Ebola virus, and of
Leishmania amastigotes. CD209 is expressed by immature DCs in peripheral tissues, with
the noticable exception of Langerhans cells. It is also expressed by DCs in lymphoid
tissues, and by certain macrophages. In peripheral blood, a percentage of plasmacytoid
DCs (or DC2) may also express CD209. It is totally absent from monocytes, and an
observed up-regulation of CD209 expression on monocyte-derived DCs correlates with
the down-regulation of CD14. Monocyte-derived macrophages may express CD209, only
when cultured in the presence of IL-13.
CD209 (DC-SIGN)
CD209 expression on DCs derived from
monocytes cultured in
GM-CSF + IL-4.
Clone: AZND1 Isotype: IgG1 Mouse
The AZND1 monoclonal antibody (mAb) is useful for many purposes, including flow cytometric analysis of CD209-expressing cells,
immunohistochemical analysis of CD209
+
cells on frozen tissue sections, immunoprecipation of CD209 antigen, and investigation of its
functions, due to the blocking or inhibiting properties of AZND1 mAb. The AZND1 mAb to DC-SIGN has been assigned to the CD209
cluster of differentiation during HLDA 7.
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CD226 is also known as: Platelet and T-cell activation antigen 1 (PTA-1) and T lineage-
specific activation antigen 1 (TLiSA1). When bound to its ligand, DNAM-1 triggers
cytotoxicity mediated by NK and T cells through the association within lipid bilayer rafts
with CD11a/CD18 (LFA-1). DNAM-1 mediates platelet and megakaryocyte cell adhesion
to the vascular endothelial cell. DNAM-1 regulates monocyte extravasation via its
interaction with CD155 (PVR) expressed at intercellular junctions in vascular endothelial
cells. CD226 is expressed by platelets, a majority of NK cells, a fraction of T cells, NKT
cells, some B cells, and by some monocytes, basophils and eosinophils.
CD226 (DNAM-1)
Clone: KRA236 Isotype: IgG1 Mouse
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Human CDs
CD235a, Glycophorin A, is a major sialoglycoprotein expressed on human red blood cells and erythroid precursor cells.
CD235a (Glycophorin A)
Clone: 11E4B-7-6 (KC16) Isotype: IgG1 Mouse
The 11E4B-7-6 (KC16) antibody binds to the amino acid sequence 27-39 of glycophorin A and has no reactivity to glycophorin B. It
reacts with immature erythrocytes including erythroblasts, proerythroblasts and reticulocytes. Staining pattern: membrane.
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The CD243 antigen (P-gp) is the 170-180 kDa multi-drug resistance (mdr)-1 gene
product. This protein carries out ATP-dependent efflux of structurally diverse lipophilic
compounds, including many anti-cancer chemotherapy agents. In hematopoietic cells, the
mdr1 gene product is expressed, but at relatively low levels. P-gp is expressed in
specialized epithelial cells with secretory or excretory functions. In the liver, P-gp is found
on the biliary surface of hepatocytes and small biliary ductules; in the pancreas, on the
laminal surface of epithelial cells of small ductules and, in the kidney, on the brush border
of the proximal tubules. Additional sites of expression include the gastrointestinal tract,
endothelial cells of the brain and the adrenal glands.
CD243 (P-glycoprotein)
PGP-negative K562 cell line (unshaded
histogram) and adryamicin-resistant K562
(shaded histogram).
Clone: UIC2 Isotype: IgG2a Mouse
The UIC2 antibody reacts with an external epitope of the human P-glycoprotein (P-gp). UIC2 antibody inhibits Pgp-mediated efflux and
potentiates the cytotoxic action of P-gp transported drugs in MDR cells. Staining pattern: membrane.
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Human CDs
The CD244 antigen, also called 2B4, is a 38 kDa cell surface signal transduction molecule
found on almost all human NK cells. It induces a granule independent killing mechanism by
NK effector cells. It is also found on TCR T cells and appoximately half of peripheral
TCR CD8
+
T cells. It is not found on B or nave CD8
+
T cells. Upon activation, most CD8
+
T cells become CD244-positive. However, a small but significant minority of CD8
+
T cells
remain CD244-negative. This subset is associated with lower cytotoxic activity, lower -
IFN production and higher IL-4 and IL-10 production. CD244 is a high affinity receptor for
CD48.
CD244 (2B4)
lymphocytes.
Clone: C1.7 Isotype: IgG1 Mouse
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The CD246 antigen, or ALK (Anaplastic Lymphoma Kinase; neural tyrosine kinase) is a
200 kDa transmembrane molecule expressed only in neural tissues (weakly) and by the
rhabdomyosarcoma Rh30 cell line. The alk gene is on chromosome 2. Upon translocation
between chromosome 2 and chromosome 5 [t(2;5)], the alk gene fuses with the npm
gene. NPM, also known as nucleophosmin or B23, is an ubiquitous nucleolar
phosphoprotein involved in the transport of pre-ribosomal components between the
nucleolus and the cytoplasm. The chimeric product resulting from t(2;5) translocation is a
protein of 80 kDa with the N-terminal portion of NPM linked to the complete intracellular
portion of ALK. Karpas 299 and SU-DHL-1 lymphoid cell lines strongly express NPM-ALK.
CD246 (ALK)
ALK-positive anaplastic large cell
lymphoma bearing the ALK-variant fusion
protein other than NPM-ALK (t(2;5)-
negative). (APPAP): ALKc staining is
confined to cytoplasm.
Clone: 62.463.2D5.2 (ALKc) Isotype: IgG1 Mouse
This antibody reacts with the normal ALK protein as well as with the chimeric protein ALK-NPM. ALKc, directed against the cytoplamic
portion of ALK, labels a 200 kDa band on Western blots of Rh30 cells, and a 80 kDa band on Western blots of Karpas 299 cells. Tissue
reactivity: on fixed, parrafin-embedded tissue sections, ALKc specifically labels t(2,5)-positive cells giving a strong and diffuse
cytoplasmic positivity, often associated with a nucleolar staining. Staining pattern: cytoplasmic, often associated with nucleolar.
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Human CDs
The CD272 antigen is a type I transmembrane glycoprotein which is also known as B and
T lymphocyte attenuator (BTLA). Immune cell function implicates important modulators
known as cosignaling receptors. On T cells, the cosignaling receptors belong to either the
immunoglobulin (CD28-like) or TNF receptor (TNFR) superfamilies. CD272 belongs to the
CD28 immunoglobulin superfamily which is composed of coinhibitory (CD28, ICOS) and
costimulatory receptors. CD272 is one of the three coinhibitory receptors with Cytotoxic T
Lymphocyte Antigen 4 (CTLA4) and Programmed Death-1 (PD-1). It has also been
reported that BTLA displays T cell inhibition via interaction with HVEM (Herpes Virus Entry
Mediator) which belongs to the tumor necrosis family (TNF) receptors demonstrating an
interaction between the primary T cell cosignaling receptors of both CD28 and TNF
receptors families. Whereas BTLA is not expressed by naive T cells, it is induced during
activation and decreases upon T cell activation. It remains expressed on T helper type 1
but not T helper type 2 cells. It is expressed on activated B cells but also on dendritic cells,
NK cells or macrophages, as demonstrated on mice.
CD272 (BTLA)
Lyzed normal whole blood sample.
Clone: BTLA7.2 Isotype: IgG2b Mouse
The BTLA7.2 monoclonal antibody recognizes T and B lymphocytes, dendritic cells and NK cells. It has been assigned to the CD272
cluster of differentiation during the 8th HLDA Workshop on Human Leukocyte Differentiation Antigens, held in Adelaide, Australia, in
2004.
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The CD273 antigen is also known as Programmed Cell Death 1 ligand 2 (PDCD1LG2 or
PD-L2) or B7-DC. It is encoded by a gene located on the human chromosome 9p24.2.
Immune cell function implicates important modulators known as cosignaling receptors. On
T cells, the cosignaling receptors belong to either the immunoglobulin (CD28-like) or TNF
receptor (TNFR) superfamilies. The CD28 immunoglobulin-superfamily is composed of
coinhibitory (CD28, ICOS) and costimulatory receptors. Programmed Death- 1 (PD-1) is
one of the three coinhibitory receptors with Cytotoxic T Lymphocyte Antigen 4 (CTLA4)
and CD272. CD273 antigen is one of the two ligands, with PD-1 ligand 1 (PD-L1), for PD
-1 and belongs to the B7 family. The PD-1 - PD-L1/PD-L2 interaction plays a critical role
in regulating T cell activation and tolerance. Whereas PD-L2 can act as a negative
regulator of T cell activity by inhibiting T cell receptor (TCR)-mediated proliferation and
cytokine production by CD4+ T cells, it can also inhibit 1 and 2 integrin-mediated
adhesion. CD273 antigen is expressed on immature dendritic cells, mature dendritic cells,
IFN- - treated monocytes, and follicular dendritic cells.
CD273 (PD-L2)
HUVECS cells.
Clone: PD-L2 Isotype: IgG1 Mouse
The PD-L2 monoclonal antibody recognizes dendritic cells, T cells and follicular dendritic cells. Staining pattern: membrane.
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Human CDs
The CD274 antigen is known as Programmed Cell Death 1 ligand 1 (PDCD1LG1 or PD-
L1) or B7-H1. It is encoded by a gene located on the human chromosome 9p24. Immune
cell function implicates important modulators known as cosignaling receptors. On T cells,
the cosignaling receptors belong to either the immunoglobulin (CD28-like) or TNF
receptor (TNFR) superfamilies. The CD28 immunoglobulin-superfamily is composed of
coinhibitory (CD28, ICOS) and costimulatory receptors. Programmed Death- 1 (PD-1) is
one of the three coinhibitory receptors with Cytotoxic T Lymphocyte Antigen 4 (CTLA4)
and CD272. CD274 antigen is one of the two ligands, with PD-1 ligand 2 (PD-L2), for PD
-1 and belongs to the B7 family. The PD-1 - PD-L1 interaction plays a critical role in
regulating T cell activation and tolerance. In particular, it plays a role during development
by inhibiting the TCR-mediated positive selection of thymocytes. It has also been shown to
be implicated in the regulation of CD4 positive T cells proliferation. CD274 antigen is
expressed on immature dendritic cells (iDC), mature dendritic cells (mDC), IFN- -treated
monocytes, and follicular dendritic cells.
CD274 (PD-L1)
Peripheral Mononuclear Blood Cells
(PBMC) stimulated with PHA
during 72h.
Clone: PD-L1 Isotype: IgG1 Mouse
The PD-L1 monoclonal antibody recognizes dendritic cells, T cells and follicular dendritic cells. It has been assigned to the CD274 cluster
of differentiation during the 8th HLDA Workshop on Human Leukocyte Differentiation Antigens, held in Adelaide, Australia, in 2004.
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The CD278 antigen, also known as ICOS molecule (inducible T-cell costimulator, Inducible Costimulator), is a disulfide-linked
homodimeric T-cell surface glycoprotein of 55-60 kDa, which belongs to the CD28 and CTLA-4 cell-surface receptor family.
The CD278 molecule plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. While
CD278 antigen is not constitutively expressed on nave peripheral blood T cells, it is highly expressed on unstimulated
thymocytes of germinal centers, showing a role in T cell differentiation. Upon activation by antigen presenting cells, T cells
(CD4 positive cells and CD8 positive cells) express inducible co-stimulatory receptors, including CD28, CTLA4 and ICOS.
The resulting interaction of CD278 (ICOS) with its ligand ICOS-L (B7-like molecule present on B cells) participates to
interleukin production, especially IL-10 and IL4. The central role of CD278 in the generation and maintenance of humoral
immunity has also been shown to be important. Upon the co-stimulation of T cells through ICOS and CD28, ICOS binds its
ligand ICOS-L leading to IgG and IgM secretion as well as IgE production. It is not expressed on resting peripheral T cells, B
cells, NK cells, monocytes/macrophages, platelets and granulocytes.
CD278 (ICOS)
Clone: ISA-3 Isotype: IgG1 Mouse
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Human CDs
The CD279 antigen, also known as Programmed Death 1 (PD-1), belongs to the
immunoglobulin superfamily. It contains an immunoreceptor tyrosine-based inhibitory motif
(ITIM) in its cytoplasmic tail. It is located on the human chromosome 2p37. Immune cell
function implicates important modulators known as cosignaling receptors. On T cells, the
cosignaling receptors belong to either the immunoglobulin (CD28-like) or TNF receptor
(TNFR) superfamilies. The CD28 immunoglobulin-superfamily is composed of coinhibitory
(CD28, ICOS) and costimulatory receptors. Programmed Death- 1 (PD-1) is one of the
three coinhibitory receptors with Cytotoxic T Lymphocyte Antigen 4 (CTLA4) and CD272.
CD279 antigen is the receptor for both ligands, PD-1 Ligand 1 (PD-L1) and PD-1 Ligand
2 (PD-L2). The PD-1 - PD-L1/PD-L2 interaction plays a critical role in regulating T cell
activation and tolerance.
CD279 (PD-1)
Clone: PD1 Isotype: IgG2b Mouse
The PD1 monoclonal antibody recognizes T cells, activated CD4 and CD8 positive cells and activated B cells. It has been assigned to the
CD279 cluster of differentiation during the 8th HLDA Workshop on Human Leukocyte Differentiation Antigens, held in Adelaide,
Australia, in 2004. Staining pattern: membrane.
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Human CDs
CD294, also known as CRTH2, is a seven-transmembrane G-protein-coupled receptor
known as the chemoattractant receptor-homologous molecule expressed on Th2 cells. The
deduced amino acid sequence of CRTH2 suggests two potential N-glycosylation sites at
the first extracellular domain and a unique long cytoplasmic tail including four consensus
sites for protein kinase C phosphorylation. Two G protein-coupled receptors,
Prostaglandin D receptor (DP), and CRTH2 have been identified as receptors for
Prostaglandin D2 (PGD2), but they differ in their signaling pathways. PGD2 is the major
metabolite of arachidonic acid produced by allergen-activated mast cells and has been
implicated in various allergic diseases as a proinflammatory lipid mediator. CRTH2
molecule is preferentially expressed on human T-helper (Th) 2 and T cytotoxic (Tc) 2 cells
but not on Th1 and Tc1 cells. CRTH2 is the most reliable surface marker selectively
associated with circulating T (Th and Tc) cells able to produce IL-4 (as well as IL-5 and IL
-13) and not IFN. On normal whole blood leucocytes, CD294 is highly expressed on
basophils and eosinophils, as well as Th2 and Tc2 cells. The expression of CD294 in
peripheral blood mononuclear cells (PBMCs) is highly restricted to an activated state of
Type 2 cells: i.e. CD25
neg
T cells do not express CD294. CD294 may be expressed
(weakly) on some monocytes and/or dendritic cells (CD14
dim
,CD16
pos
,HLA-DR
pos
,CD33
pos
).
CD294 may act in mediating the recruitment and/or activation of basophils, eosinophils
and Th2 cells at sites containing mast cells activated by invading allergens. CRTH2, but
not DP, induces migration of Th2 cells, eosinophils and basophils in response to PGD2.
CD294 (CRTH2)
Clone: BM16 Isotype: IgG2a Rat
The BM16 monoclonal antibody precipitates a 55 to 70 kDa protein from cell lysates of CRTH2-transfected Jurkatt and Th2 clone,
corresponding to PGD2 receptor.
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The CD300a, also known as inhibitory receptor protein (IRp60), is a surface molecule of
60kDa expressed by all human natural killer (NK) cells. The IRp60 gene, located on human
chromosome region 17q25 encodes a molecule, highly O- and N-glycosylated, belonging
to the immunoglobulin superfamily (Ig-SF) that displays one extracellular Ig-like domain of
the V-type, a hydrophobic transmembrane portion and a cytoplasmic tail containing ITIM
motifs involved in the inhibitory function. The ligand of the CD300a is still unknown but
CD300a does not appear to recognize HLA-class I molecules. Upon cross-linking, IRp60
recruits and activates SH2-containing phosphatases SHP-1 and SHP-2 which switch off
the activating signaling cascade by dephosphorylating a variety of tyrosine phosphorylated
proteins. Cross-linking of IRp60 by E59.126 monoclonal antibody strongly inhibits the
spontaneous cytotoxicity of NK cells as well as the NK-mediated cytolytic activity induced
via different non-HLA-specific or HLA-specific activating receptors. CD300a may play a
more general role in the regulation of the immune response since it is expressed on other
cell types, including T lymphocyte subsets, monocytes and granulocytes.
CD300a (IRp60)
Clone: E59.126 Isotype: IgG1 Mouse
The E59.126 antibody has been used in flow cytometry and Western blotting to characterize IRp60.
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Human CDs
CD309 antigen, also called KDR (Kinase insert domain receptor), or flk-1 (Fetal liver
kinase 1) belongs to the VEGF (vascular endothelial growth factor) receptor family. It is
known as the VEGFR-2 and is one of the three cell signaling tyrosine-kinase receptors of
the VEGF family [VEGFR-1 (flt-1), VEGFR- 2 (KDR/flk-1), VEGFR-3 (flt-4)] and plays a
key role in vascular development and regulation of vascular permeability. These receptors
are transmembrane proteins that contain seven immunoglobulin-like domains in their
extracellular part and two tyrosine kinase domain in their intracellular part. The KDR gene
maps to the chromosome 4q11-q12. CD309 is the receptor for VEGF but it also binds
VEGF-C, VEGF-D, and VEGF-E. Through the binding to CD309, which is expressed
almost in endothelial cells, VEGF induces endothelial cell proliferation, and promotes cell
migration. CD309 is expressed during early stages of embryogenesis. It has also been
shown that VEGF can induce, in vivo, angiogenesis as well as permeabilization of blood
vessels, and plays a central role in the regulation of vasculogenesis. It is expressed on
CD34 positive cells defining a CD34
+
CD309
+
common precursor for both hematopoietic
cells and endothelial cells called hemoangioblast.
CD309 (KDR)
KDR transfected cells.
Clone: KDR-1 Isotype: IgG1 Mouse
The KDR-1 monoclonal antibody was assigned to the CD309 cluster of differentiation at the 8th International Workshop on Human
Leucocyte Differentiation Antigens in Adelaide, Australia, in 2004.
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CD314, also known as NKG2D, is a 42 kDa type II transmembrane protein belonging to
the C-type lectin superfamily. The name NKG2D may be misleading as it is not a typical
NKG2 family member. It has low homology with the other members and dimerizes with
itself to form a homodimer while other members dimerize with CD94. The surface
expression of NKG2D requires association with a signaling adaptor protein, a KAP10
(DAP10) homodimer that contains a Y-x-x-M motif. Upon tyrosine phosphorylation, that
motif recruits and activates phosphatidylinositol 3 (PI 3)-kinase. Unlike natural cytotoxicity
receptors (NCRs), NKG2D is not only expressed by all NK cells but also by most T
cells and by virtually all CD8
+
T cells. In addition, its expression can be up-regulated on
NK cells by different cytokines (IL-15, IL-12 and IFN-). Human CD314 ligands identified
so far are MICA and MICB, stress-inducible and broadly expressed molecules on tumors
of epithelial origin, some melanomas, and ULBPs, targets of the human CMV protein,
expressed on a wide range of cells. NKG2D triggering would appear to overrule the
inhibitory signals delivered by the KIR and HLA class 1 interactions. NKG2D might play a
complementary role in the NK-cell mediated lysis of target cells that appear to express
HLA class I molecules normally and that express one or more NKG2D-specific ligands.
CD314 (NKG2D)
lymphocytes.
Clone: ON72 Isotype: IgG1 Mouse
The ON72 antibody has been used in flow cytometry to analyze the expression of NKG2D on NK cells.
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Human CDs
E-cadherin, also called L-CAM (Liver Cell Adhesion Molecule) is a 123 kDa molecule of the cadherin family, which mediates
Ca
2+
-dependent homotypic adhesion between epithelial cells. It is involved in the generation and maintenance of epithelial
layers during development and in adult tissues.
CD324 (E-cadherin)
Tissue reactivity: the 67A4 antibody recognizes E-cadherin expressed by non-neural epithelial tissues. Staining pattern: membrane.
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CD335, also known as NKp46, is a 46-kDa transmembrane glycoprotein which is a NK-
specific triggering receptor involved in non-MHC-restricted natural cytotoxicity and
considered the prototype of the NK natural cytotoxicity receptors (NCRs). Its expression is
restricted to all resting and activated NK cells, including the minor CD3
CD56
bright
CD16
subset. Although CD335 is highly expressed at the NK-cell surface in the majority of
donors, some individuals have a proportion of NK cell (which vary up to 90%) expressing a
dull NKp46 phenotype. CD335 is a member of the immunoglobulin superfamily
characterized by two extracellular C2-type Ig-like domains. It is associated with the ITAM
bearing molecules CD3 and FcRI. Although this association is likely to be essential for
signal transduction via NKp46, CD3 is not required for NKp46 surface expression.
NKp46 represents a major activating receptor and plays a central role in the lysis and
clearance of HLA class I
cells. The magnitude of lysis correlates with its level of surface

expression.
CD335 (NKp46)
Clone: BAB281 Isotype: IgG1 Mouse
BAB 281-mediated masking of NKp46 molecule inhibits NK cytotoxicity versus different target cells. In redirected killing assays, BAB281
binding to NKp46 induces a strong increase of cytolytic activity. Although hemagglutinins of viral origins have been identified as ligands
for NKp46 receptor, the natural (non-viral) ligand for NKp46 is unknown.
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Human CDs
CD336, also known as NKp44, is a 44-kDa transmembrane glycoprotein which is
selectively expressed by activated NK cells. NKP44 functions as a triggering receptor
involved in non-MHC-restricted natural cytotoxicity by activated NK cells and is one of the
NK natural cytotoxicity receptors (NCRs). Structurally, CD336 is a member of the
immunoglobulin superfamily characterized by a single extracellular V-type Ig-like domain. It
associates with a homodimer of ITAM motif-containing KARAP / DAP12 adaptor proteins.
These adaptor proteins were found to be crucial both for the surface expression of NKp44
and for transducing activating signal. CD336 can mediate triggering of NK cell cytotoxicity
as in redirected killing assays. Monoclonal antibody Z231-mediated cross-linking of
NKp44 in cloned NK cells resulted in strong lysis of target cells and, Z231-mediated
masking of NKp44 partially inhibits cytolytic activity. Such inhibition is greatly increased if
an anti-CD335, also known as anti-NKp46 (BAB281), is combined with Z231. The
CD336 ligand is still undefined. Since activated NK cells selectively express NKp44, it may
play a partial role in the enhanced cytotoxicity of IL2-activated compared to those freshly
isolated NK cells from peripheral blood.
CD336 (NKp44)
Clone: Z231 Isotype: IgG1 Mouse
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CD337, also known as NKp30, is a 30-kDa transmembrane glycoprotein which is a NK-
specific triggering receptor involved in non-MHC-restricted natural cytotoxicity. NKp30 is
strictly expressed by all resting and activated NK cells including the minor CD3
CD56
bright
CD16
subset. With NKp46 and NKp44 it is one of the three identified NK

natural cytotoxicity receptors (NCR). Its surface expression parallels that of NKp46.
NKp46
dull
NK cells are also characterised by a NKp30
dull
phenotype while NKp46
bright
NK
cells show a NKp30
bright
phenotype. The NKp30
bright
or NKp30
dull
phenotypes are correlated
with high or low cytotoxicity, respectively. CD337 is a member of the immunoglobulin
superfamily characterized by a single extracellular V-type Ig-like domain. It is associated
with a homodimer of ITAM motif-containing CD3 adaptor proteins. These adaptor
proteins are not necessary for surface expression of NKp30, but are necessary for signal
transduction. In redirected killing assays, Z25-mediated cross linking of NKp30 induces
strong NK cell activation. Conversely, Z25-mediated masking of NKp30 inhibits NK
cytotoxicity against target cells. More importantly, CD337 represents the major receptor in
induced NK-mediated killing of certain target cells, the lysis of which is largely
NKp46 / NKp44 independent. Although the CD337 ligand is unknown, studies report
NKp30-mediated lysis of immature dendritic cells (iDC). These findings suggest that DCs
express one or more unidentified ligands for CD337.
CD337 (NKp30)
Clone: Z25 Isotype: IgG1 Mouse
The Z25 antibody has been used in flow cytometry to analyze the expression of NKp30 on NK cells.
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Human non CDs
7.1 (NG2 antigen)
Human melanoma-associated chondroitin suflate proteoglycan (MCSP), known as the
homolog rat NG2 antigen, is a 250 kDa glycoprotein that is though to play important roles
in angiogenesis-dependent processes like wound healing and tumor growth. The core
protein contains an open frame of 2322 amino acids encompassing a large extracellular
domain, a hydrophobic transmembrane region and a relatively short cytoplasmic tail. This
antigen is not expressed on normal hematopoietic cells but can be expressed on myeloid
and lymphoid cell subsets with chromosomal modifications. It is also expressed on human
glial cells. This antigen regulates signaling events that are important for both cell
proliferation and cell migration through at least two distinct mechanisms: the focal
adhesion kinase (FAK) and the extracellular signal-regulated kinases (ERK) 1/2.
HELA cell line.
The 7.1 monoclonal antibody recognizes the human melanoma associated chondroitin sulfate proteoglycan molecule.
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Akt (PKB)
Akt, also known as protein Kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of
Akt: Akt1 (PKB ), Akt2 (PKB ) and Akt3 (PKB ) with Akt2 and Akt3 being approximately 82% identical with the Akt1 isoform.
Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy-terminal regulatory domain. Akt is a
downstream target of phosphatidylinositol 3-kinase (PI-3K) activation through the phosphorylation of Akt at two residues
following the interaction of 3-phosphorylated phosphoinositides, such as PtdIns(3,4)P2 and Ptdlns (3,4,5)P3 via PH domains.
Akt has been shown to play a role in such metabolic processes as glucose transport, glycogen synthesis, glycolysis, and
protein synthesis. It had also been shown to promote cell survival by inhibiting apoptosis through its ability to phosphorylate
and inactivate several targets, including BAD, Forkhead transcription factors, and caspase-9.
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Human non CDs
-Feto Protein
The mammalian -fetoproteins (AFPs) are single chain glycoproteins with molecular
weight ranging from 66 to 72 kDa. They are one of the major serum proteins in the early
life. The function of these proteins is not yet fully understood. They transport a multitude of
ligands and are largely growth promoters even if post-translationally modified AFP
molecules have been reported to possess inhibitory properties. AFP is expressed at high
levels in the embryonic yolk sac and fetal liver and decreases dramatically after birth.
Traces of AFP are synthesized in the adult liver. However, expression of the AFP gene is
reactivated in the adult during liver regeneration and hepatocarcinogenesis.
Embryonic Tissue (DAB)
Clone: C3 Isotype: IgG2a Mouse
Tissue reactivity: The C3 monoclonal antibody reacts with embryonic cells. Staining pattern: cytoplasm.
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APO2.7 (7A6)
The APO2.7 antigen (also named 7A6 antigen) recognized by clone 2.7A6A3 is a 38 kDa
protein localized on the membrane of mitochondria whose expression appears to be
restricted to cells undergoing apoptosis. The APO2.7 antigen can be detected after
apoptosis induction via CD95/Fas ligation, irradiation or drug treatment. Expression of
APO2.7 appears as an early event in the apoptosis process. Normal viable cells are
negative or weakly positive for APO2.7. Research studies have shown that less than 2% of
peripheral T cells from normal donors expressed the APO2.7 antigen. Some level of
APO2.7 expression associated with an on-going apoptotic process has been
demonstrated in activated T cells.
Apoptotic Jurkat cell line.
Clone: 2.7A6A3 (APO2.7) Isotype: IgG1 Mouse
The APO2.7 antibody can be used for the direct quantitation of apoptotic cells by flow cytometry, after
permeabilization with digitonin. Use of APO2.7 allows for precise monitoring of the early and late cellular responses
during apoptosis.
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Human non CDs
Bad
The Bcl-2 related proteins can inhibit (Bcl-XL and Mcl-1) or induce (Bax, Bcl-XS, Bag, and Bad) apoptosis in several systems.
Bad was identified as a Bcl-2 interacting protein. It has homology to Bcl-2 within the Bcl-2 homolog domains 1 and 2 (BH1
and BH2). In mammalian cells, Bad selectively heterodimerizes with Bcl-XL as well as Bcl-2, but not with other Bcl-2 family
members [Bax, Bcl-Xs, Mcl-1 and Bfl-1 (also called A1)]. When Bad forms heterodimers with Bcl-XL, it displaces Bax from
Bcl-XL and promotes cell death.
Clone: Polyclonal Isotype: Ig Rabbit
This antibody reacts with the 30 kDa mouse and the 25 kDa human Bad, by Western blot, using total cell lysate from
mouse NIH3T3, WR19L12a and human A431, HL60 cell lines.
- Freeze-dried RUO A32526 0.1 mg Purified
Bax
Bax is a 21 kDa protein that has extensive amino acid homology with Bcl-2. It forms homodimers or heterodimers with Bcl-2.
Apoptotic activity depends on a balance between both molecules. Overexpression of Bax accelerates programmed cell death
induced by apoptotic stimulus.
Clone: 2D2 Isotype: IgG1 Mouse
Cell Lab Liquid RUO 731730 0.1 mg UNLB
MAb 6A7 reacts with human, mouse and rat Bax but does not bind the soluble cytosolic form of Bax; however,
treatment of cells with non-ionic detergents exposes the epitope and allows binding of 6A7 to monomeric forms of Bax
but not Bax complexed with either Bcl-2 or Bcl-xL.
Bcl-2
Bcl-2 is a 25 kDa protein that has extensive amino acid homology with Bax. It forms homodimers or heterodimers with Bax and
the apoptotic activity depends on the balance of both molecules. Bcl-2 oncoprotein is an integral mitochondrial membrane
protein encoded by the genes involved in the t(14; 18) translocation.
Clone: 83-8B Isotype: IgG1 Mouse
The 83-8B antibody can be used to detect intracytoplasmic Bcl-2 by flow cytometry after permeabilization with
IntraPrep reagent. 83-8B reacts with rat Bcl-2 and human Bcl-2. Staining pattern: cytoplasm.
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Human non CDs
Bcl-xL
Bcl-xL is a 29 kDa member of the Bcl-2 family of proteins involved in regulation of programmed cell death, or apoptosis. It is
found in both soluble and membrane fractions of cellular lysates. Like Bcl-2, Bcl-xL has been demonstrated to block apoptosis
which is induced by a variety of stimuli and, under certain conditions, offers greater protection against apoptosis than Bcl-2.
While formation of heterodimers with the apoptosis-enhancing protein, Bax, does not block the apoptosis-suppressing
property of Bcl-xL, heterodimerization with Bad, a recently described member of the Bcl-2 family, effectively inhibits the
protective function of Bcl-xL.
Clone: 7B2.5 Isotype: IgG3 Mouse
MAb 7B2.5 reacts with human, mouse and rat Bcl-xL.
C
Cell Lab Liquid RUO 731722 0.1 mg BIOT
Cell Lab Liquid RUO 731721 0.1 mg FITC
Cell Lab Liquid RUO 731723 0.1 mg PE
Clone: 2H12 Isotype: IgG2a Mouse
2-microglobulin
The 2-microglobulin is the non-polymorphic non-covalently bound light chain (12 kDa) of the class I major histocompatibility
complex (MHC-I) constitutively expressed on the surface of almost all nucleated cell. 2-microglobulin structure, stabilized by a
disulfide bound, is similar to an immunoglobulin like domain. 2-microglobulin is required in the endoplasmic reticulum (ER) to
associate with the MHC class I heavy molecule to form with other proteins (calreticulin, tapasin, etc.) the loading complex that
exits the ER and is transported to the cell surface. After peptide presentation, 2-microglobulin is actively released into
circulation.
Clone: B1G6 Isotype: IgG2a Mouse
The B1G6 monoclonal antibody is specific for human 2-microglobulin. It does not react with 2-microglobulin from
other species. It recognizes the 2-microglobulin, free or associated with human HLA class I molecules.
C
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Human non CDs
Caspase 8
Caspase 8 (FLICE/MACH/Mch5) is a member of the ICE (interleukin-1 converting enzyme)/CED-3 cysteine protease family.
It is the most upstream protease that receives the activation signal from the CD95/Fas molecule and TNFR.I (tumor necrosis
factor receptor 1) to initiate the apoptotic protease cascade that leads to activation of the ICE/CED-3 family proteases. The
binding of Caspase 8 to FADD (MORT1) through the association of their death effector domains (DED), and the consequent
activation of the caspase pathway by proteolytic cleavage, are thought to be critical steps in the initiation of Fas- and TNFR.I-
induced apoptosis.
Clone: 5F7 Isotype: IgG2b Mouse
The 5F7 antibody detects, on Western blot, two bands of 55 kDa and 54 kDa, corresponding to human caspase 8a
(MACHa1) and to human caspase 8b (MACHa2), respectively.
W
c-erbB-2
c-erbB-2 is a cell surface protein-tyrosine kinase receptor of 185 kDa. It is a member with
the epidermal growth factor (EGF) receptor, c-erbB-3 and c-erbB-4 of the type 1 family of
growth factor receptors. Activation of c-erbB-2 receptor requires heterodimerization with
EGF receptor, c-erbB-3 or c-erbB-4.
Breast carcinoma (DAB)
Clone: 3B5E7.2 Isotype: IgG1 Mouse
The 3B5E7.2 monoclonal antibody recognizes the internal domain of c-erbB-2, it does not react with EGF receptor (gp
-170).
P F I
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Human non CDs
Collagen Type I
Collagens are generally extracellular structural proteins involved in formation of connective tissue structure. The primary
structure of collagen is a polypeptide consisting of the repeating sequence (glycine-X1-X2)n where X1 is often proline and X2
a hydroxyproline. This long molecule twists into an elongated, left-handed helix. When synthesized, the N- and C-terminal have
globular domains which keep the molecule soluble. As the molecule passes through the endoplasmic reticulum and Golgi
apparatus, the molecule is glycosylated, hydroxyl (-OH) groups are added and S-S bonds link three chains covalently. The
three molecules twist together to form a triple helix.
Collagen I, found in bone, cornea, skin and tendon, is composed of two alpha1(I) molecules and one alpha2(I) molecule.
Clone: 2A3 Isotype: IgG2b Mouse
Clone: 4F6 Isotype: IgG2b Mouse
Clone: Polyclonal Isotype: Ig Goat
E F
Collagen Type III
Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system.
Collagen type III is characterized by the fact that the three molecules (alpha1(III)) are identical.
E F
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Human non CDs
Collagen Type IV
E F
Collagen Type V
Collagen V is composed of several subunits as follows: two alpha1(V) and one alpha2(V) molecules in most tissues and one
alpha1(V), one alpha2(V), and one alpha3(V) molecules in placenta.
E F
Collagen Type VI
Collagen VI is a major structural component of microfibrils. The basic structural unit is a heterotrimer of the alpha1(VI), alpha2
(VI), and alpha3(VI) molecules.
E F
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Human non CDs
Cyclin A2
In humans, there are two A-type cyclins an embryonic-specific cyclin A1 and a somatic
cyclin A2. Cyclin A1 is only expressed in meiosis and very early embryos, whereas cyclin
A2 is present in proliferating somatic cells. Cyclin A2 is a 48 kDa protein composed of
432 amino-acids. Cyclins control the activation of cyclin dependent kinases (CDKs) by
associating with them to form a cyclin-CDK complex able to phosphorylate selected
proteins and to induce downstream processes. The cyclin A2-CDK2 complex initiates
DNA replication and is required for S phase progression. For passage through G2 to M
phase, cyclin A2 complexes to the CDK1 (also called CDC2). Cyclin A2 is degraded via
the ubiquitin-dependant proteolysis pathway in the early phases of mitosis.
Human lymphoid cell line (CEM).
Clone: 11B2G3 Isotype: IgG1 Mouse
The 11B2G3 antibody reacts with human cyclin A2.
C
Cyclin D1
Cyclin D1, also called PRAD1/Cyclin D1, is a 36 kDa protein which regulates cyclin-dependent protein kinase activity in the
G1 phase of the cell cycle.
Clone: 5D4 Isotype: IgG2a Mouse
The 5D4 antibody recognizes both human and mouse cyclins D1 and D2. It does not react with human and mouse
cyclin D3. Tissue reactivity: overexpressed in several neoplasms. Staining pattern: nuclear/cytoplasmic.
P F P E W
- Liquid RUO IM1740 0.1 mL Purified
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Human non CDs
Cytokeratin
Cytokeratins belong to the intermediate filament protein family and are characteristic of
epithelial cells. At present, more than 20 different cytokeratins have been identified based
on the original two dimensional gel electrophoresis classification. A sub classification,
based on charge, divides cytokeratins into two subfamilies: the acidic (negatively charged)
cytokeratin (type 1) and the neutral to basic (positively charged) cytokeratin (type II). They
are typically expressed as pairs, consisting of one acidic and one basic molecule.
HEP2 cell line.
Clone: J1B3 Isotype: IgG1 Mouse
This monoclonal antibody exclusively reacts with cells of epithelial origin, and shows a cytoplasmic staining pattern. It
can be used as a gating tool in flow cytometry to distinguish epithelial from non-epithelial cells. Multi-color flow
cytometric analysis requires preliminary permeabilization of the cells with digitonin or methanol.
C
Cytokeratins-large spectrum
Cytokeratins belong to the intermediate filament protein family and are characteristic of
epithelial cells. At present, more than 20 different cytokeratins have been identified based
on the original two dimensional gel electrophoresis classification. A sub classification,
based on charge, divides cytokeratins into two subfamilies: the acidic (negatively charged)
cytokeratin (type 1) and the neutral to basic (positively charged) cytokeratin (type II). They
are typically expressed as pairs, consisting of one acidic and one basic molecule.
Cytokeratin 8, 18 and 19 are the most abundant in simple epithelial cells; cytokeratins 5
and 14 are localized in basal cells and cytokeratin 2, 6, 10, 11 in the suprabasal tissue.
Micrometastasis of adenocarcinoma in
lymph node (AEC)
Clone: KL1 Isotype: IgG1 Mouse
KL1 strongly recognizes cytokeratins CK2 (65.5 kDa), CK6 (56 kDa), CK8 (52.5 kDa), CK10, CK11 (56 kDa), CK18 (45
kDa), CK19 (40 kDa) and to a lesser extent CK5 (58 kDa) and CK14/15 (50 kDa). Tissue reactivity: KL1 reacts with all
epithelial cells and keratinocytes in suprabasal layers, in both keratinizing and non-keratinizing squamous and
glandular epithelia. It does not react with epidermal basal layer cells. Staining pattern: cytoplasm.
W P F
IOPath+2 Liquid RUO IM2128 0.5 mL Purified
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Human non CDs
DAP12 (DNAX / KARAP / TYROBP)
The DAP12 antigen (DNAX activating protein of 12 kDa) is a type I transmembrane
glycoprotein which is also known as KARAP (killer cell activating receptor-associated
protein) or TYROBP (tyrosine kinase binding protein). It is encoded by a gene located on
the chromosome 19q13 and contains an intracytoplasmic immunoreceptor tyrosine-based
activation motif (ITAM). The DAP12 antigen associates with a large family of receptors in
hematopoietic cells conferring to this antigen a broad range of intracellular signaling
functions Interestingly, it has been shown to both potentiate or inhibit leukocytes activation.
When associated with KIRs (stimulatory killer Ig-like receptors), the adaptor molecule
DAP12 functions as an activation unit leading to the T cell activation. It has also been
shown that the activation of the syk/ZAP70 tyrosine kinases by DAP12 plays a role in the
stimulation of NK cells and T cells. On the contrary, the DAP12 association with other
receptors such as Fc receptor -chain and CD3 may lead to the regulation of T cell
immune response.
Transfected cells versus non transfected
cells (shown in light) stained with anti-
DAP12-PE.
Clone: H10E12F4 Isotype: IgG1 Rat
The H10E12F4 monoclonal antibody recognizes monocytes, neutrophils, dendritic cells, NK cells and to a lesser extend
basophils, and B lymphocytes. It does not recognize CD4 and CD8 T cells or B cells.
C
Estrogen Receptor
Breast pathology (DAB)
Clone: ER1D5 Isotype: IgG1 Mouse
Tissue reactivity: ER1D5 recognizes the 67 kDa polypeptide chain of estrogen receptor (ER). ER1D5 reacts with uterus
cells, with stromal, epithelial and smooth muscle cells of the endometrium. It also stains normal and hyperplastic cells
from ductal and lobular epithelium of mammary glands. Staining pattern: nucleus.
P P W F E
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Human non CDs
FADD (Fas Associated protein with Death Domain)
Fas-associated death domain (FADD, also known as MORT1) is an important apoptosis adapter molecule involved in the FAS-
mediated cell death pathway. FADD associates with the intracellular domain of Fas (CD95/APO-1) through their respective C-
terminal death domains (DD). The N-terminal region of FADD contains a death effectors domain (DED) which recruits and
binds cytolitic proteins such as FLICE/caspase-8. The subsequent activation of FLICE by proteolytic cleavage leads to the
activation of the caspase-mediated (ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosis
which is inhibited by CrmA, a specific inhibitor of the interleukin-1 - converting enzyme. FADD has also been shown under
certain circumstances to promote cell survival and proliferation.
Fibrillin
The fibrillins are extracellular matrix glycoproteins which occur as a major component of a subset of connective tissue
microfibrils. These microfibrils have a beaded appearance and a cross-sectional diameter of 10-12 nm. In elastic tissues, these
structures are thought to provide the scaffold onto which elastin is assembled to form elastic fibers, although their function in
non-elastic tissues is unclear. There is an aberrant expression of fibrillin in Marfan syndrome.
Clone: 11C1.3 Isotype: IgG1 Mouse
In immunohistochemical staining of paraformaldehyde-fixed human skin, mAb 11C1.3 labels the elastic framework and
more specifically the oxytalantic network close to the epidermis. The antibody also exhibits discrete staining of normal
fibroblasts in culture and of extracellular (elastin) fibrils in a variety of tissues.
E F C
Cell Lab Liquid LUO 731951 0.2 mg UNLB
Cell Lab Liquid LUO 731953 0.2 mg BIOT
Cell Lab Liquid LUO 731952 0.2 mg FITC
Fibronectin
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Human non CDs
FMC7 (CD20)
The recognized antigen is an epitope of the CD20 molecule. This antigen is only
expressed by mature B lymphocytes.
Clone: FMC7 Isotype: IgM Mouse
The FMC7 antibody reacts with a subpopulation of peripheral blood B lymphocytes and tonsil B cells, but it does not
react with granulocytes, monocytes, platelets, erythrocytes, T lymphocytes or null cells.
W C
G-CSF
Human granulocyte colony stimulating factor (G-CSF) is a 20 kDa glycoprotein that is produced by macrophages, fibroblasts
and endothelial cells. G-CSF regulates the production of neutrophilic granulocytes and is a growth, differentiation and
activating factor for neutrophils and their precursors.
Clone: BVD11-37G10 Isotype: IgG2a Rat
ELISA: BVD11-37G10 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying
human G-CSF protein levels. Biotinylated BVD11-37G10 antibody (PN 731778) should be paired with unlabeled
BVD13-3A5 antibody (PN 731776) as the capture antibody. It is recommended to use purified recombinant human G-
CSF as standard.
E W
Clone: BVD13-3A5 Isotype: IgG1 Rat
Western Blotting: The unlabeled BVD11-37G10 and BVD13-3A5 antibodies are also useful for Western blotting
applications, in conjunction with AP-labeled Goat Anti-Rat Ig (PN 732647).
E W
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Human non CDs
GM-CSF
Human granulocyte/macrophage colony stimulating factor (GM-CSF) is a 22 kDa glycoprotein that is produced by
macrophages, T cells, fibroblasts and endothelial cells that are activated by products such as IL-1 or TNF. GM-CSF supports
the formation of blast cells that differentiate into various lineages and is a growth factor for endothelial cells, erythroid cells,
megakaryocytes and T cells.
Clone: BVD2-21C11 Isotype: IgG2a Rat
ELISA: BVD2-21C11 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human
GM-CSF protein levels. Biotinylated BVD2-21C11 antibody (PN 731748) should be paired with unlabeled BVD2-23B6
antibody (PN 731746) as the capture antibody. It is recommended to use purified recombinant human GM-CSF as
standard.
Immunofluorescence / Flow Cytometry: BVD2-2C11 forms are useful for intracytoplasmic staining and flow cytometric
analysis to identify and enumerate GM-CSF-positive cells within mixed cell populations.
E W C
Clone: BVD2-23B6 Isotype: IgG2a Rat
Immunoprecipitation / Western Blotting: The purified BVD2-23B6 antibody is useful for immunoprecipitation and
Western blotting applications in conjunction with AP-labeled Goat Anti-Rat Ig, PN 732647.
I E W
GSK-3
Glycogen synthase kinase-3 (GSK-3) is a protein serine kinase that phosphorylates glycogen synthase and thereby inactivates
it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK-3 and subsequently
inhibits GSK-3 acutely leading to the stimulation of glycogen synthesis. GSK-3 signaling is performed by two isoforms, GSK
-3 and GSK-3 . The two isoforms share 97% sequence similarity within their catalytic domains. GSK-3 has also been shown
to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.
Clone: SBGSK32 Isotype: IgG2a Mouse
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Human non CDs
GSK-3
Glycogen synthase kinase-3 (GSK-3) is a protein serine kinase that phosphorylates glycogen synthase and thereby inactivates
it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK-3 and subsequently
inhibits GSK-3 acutely leading to the stimulation of glycogen synthesis. GSK-3 signaling is performed by two isoforms, GSK
-3 and GSK-3 . The two isoforms share 97% sequence similarity within their catalytic domains. GSK-3 has also been shown
to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.
Clone: 5D6 Isotype: IgG2b Mouse
HIV-1 core antigen
Clone: KC57 Isotype: IgG1 Mouse
The KC57 antibody identifies the 55, 39, 33 and 24 kDa proteins of the core antigens of the human immunodeficiency
virus type 1 (HIV-1). The 55 kDa protein is the precursor protein for the core antigen. The 39 and 33 kDa proteins are
intermediate products and the 24 kDa protein is the core protein.
C
COULTER CLONE Liquid RUO 6604665 100 tests FITC
HLA-ABC
The antigen corresponds to a monomorphic determinant of human HLA class I molecules. HLA-ABC is associated with -2
microglobulin. It is expressed on the surface of all human cell types.
Clone: B9.12.1 Isotype: IgG2a Mouse
The B9.12.1 antibody does not recognize unassociated heavy chains.
C
HLA-DQ
The recognized antigen is a monomorphic determinant of human class II HLA-DQ molecules. It is expressed by immuno-
competent cells, particularly B cells and macrophages.
Clone: SPVL3 Isotype: IgG2a Mouse
The SPVL3 antibody has been used in studies of inhibition of antigen-specific cytolytic T cell clones.
I N B F
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Human non CDs
HLA-DR
HLA-DR molecules are found on antigen-presenting cells (APC), i.e. dendritic cells, B
lymphocytes, monocytes, macrophages, and thymic epithelial cells. They are also
expressed on activated T cells, but not on granulocytes, platelets, or red blood cells.
Resting T cells or endothelial cells that do not normally express these molecules can be
induced to express them.
lymphocytes.
Clone: B8.12.2 Isotype: IgG2b Mouse
HLA-DR antibodies are specific to non polymorphic determinants of human HLA class II DR molecules which are
constituted of and polypeptides of 34 and 29 kDa respectively.
C
Clone: Immu-357 Isotype: IgG1 Mouse
C
IOTest Liquid ASR IM3636 1 mL ECD
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Human non CDs
HLA-DR, DP, DQ
The recognized epitope is specific to a non-polymorphic HLA class II (DR, DP, DQ) Ia antigen constituted of and
polypeptides of 34 and 29 kDa respectively. This antigen is expressed on monocytes, macrophages, B lymphocytes and
activated T lymphocytes.
Clone: 9-49 (I3) Isotype: IgG2a Mouse
C
IFN-
Human Interferon- (IFN- ) is a 20-25 kDa (monomer) pleiotrophic cytokine that is produced by activated T cells and NK cells
and is involved in the regulation of almost all immune and inflammatory responses. IFN- is involved in the activation, growth and
differentiation of T cells, B cells, macrophages, NK cells as well as other cell types. IFN- enhances MHC expression on
antigen-presenting cells including epithelial, endothelial and connective tissue cell and monocytes cell lines. It also has weak
antiviral activity and acts as a macrophage-activating factor (MAF) for tumor cytotoxicity leading to anti-tumor effects.
Clone: B27 Isotype: IgG1 Mouse
ELISA: B27 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IFN-
protein levels. Biotinylated B27 antibody (PN 731751) should be paired with unlabeled A35 antibody (PN 731749) as
the capture antibody. It is recommended to use purified recombinant human IFN- as standard.
Immunofluorescence/Flow Cytometry: B27 forms are useful for intracytoplasmic staining and flow cytometric analysis
to identify and enumerate IFN--positive cells within mixed cell populations. Immunoprecipitation: The B27 antibody
has been reported to be useful for immunoprecipitation.
C E I
Clone: A35 Isotype: IgG1 Mouse
E
The FITC- and PE-conjugated forms of the 45.15 antibody are optimized for flow cytometric detection of intracellular
IFN- in stimulated peripheral blood mononuclear cells (PBMC) after permeabilization with IntraPrep permeabilization
reagent.
C Ci
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Human non CDs
IgA1
Clone: B3506B4 Isotype: IgG1 Mouse
C
Cell Lab Liquid RUO 733194 1 mL HRP
Cell Lab Liquid RUO 733193 1 mL AP
IgA2
Clone: A9604D2 Isotype: IgG1 Mouse
C
IgD
Clone: IADB6 Isotype: IgG2a Mouse
C
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Human non CDs
IgE (D2)
Clone: E124.2.8 Isotype: IgG1 Mouse
The E124.2.8 antibody recognizes the D2 constant region of human IgE.
- Freeze-dried ASR A40174 0.2 mg Purified
IgE (Fc)
Clone: B3102E8 Isotype: IgG1 Mouse
Clone: HP6029 Isotype: IgG2a Mouse
Clone: HP6061 Isotype: IgM Mouse
IgG
Clone: H2 Isotype: IgG2b Mouse
C
Cell Lab Liquid RUO 736021 0.1 mg APC
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Human non CDs
IgG (Fc)
Clone: JDC-10 Isotype: IgG1 Mouse
C
IgG1
C
IgG2 (Fc)
Clone: 31-7-4 Isotype: IgG1 Mouse
C
Clone: HP6002 Isotype: IgG1 Mouse
C
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Human non CDs
IgG2 (Fd)
C
IgG3
C
IgG4 (Fc)
C
213
Human non CDs
IgG4 (pFc')
C
IgM
Clone: UHB Isotype: IgG3 Mouse
C
Clone: UHB Isotype: IgG3 F(ab)2 Mouse
Clone: SA-DA4 Isotype: IgG1 Mouse
C
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Human non CDs
IL-2
Interleukin-2 (IL-2) is a 15-17 kDa cytokine produced and secreted by T cells. The
induction of IL-2 secretion is the first step in T cell activation. IL-2 is the major autocrine
growth factor for activated T cells and is obligatory for the generation of cytotoxic T cells.
IL-2 also promotes the proliferation and the differentiation of NK precursors, B cells and
monocytes. Activated CD4
+
T cells of the Th1 phenotype produce predominantly IL-2 and
Interferon-, whereas CD4
+
T cells of the Th2 phenotype produce IL-4 and IL-10.
Activated PBL.
Clone: N7.48A Isotype: IgG2a Mouse
N7.48A-PE conjugated antibody is optimized for flow cytometric detection of intracellular IL-2 in stimulated peripheral
mononuclear cells (PBMC) after permeabilizing with formaldehyde/saponin-based IntraPrep Permeabilization Reagent.
C Ci
IL-3
Human Interleukin-3 (IL-3) is a 20-32 kDa haemoatopoietic growth factor that is also known as multi-CSF. IL-3 is produced by
activated T cells, mast cells and eosinophils. The most significant biological activity of IL-3 is the stimulation of hematopoietic
cell differentiation leading to the colony formation of erythroid, megakaryocyte, granulocyte, macrophage, basophil, neutrophil,
eosinophil, mast cell and monocytic lineages.
Clone: BVD3-1F9 Isotype: IgG1 Rat
ELISA: BVD3-1F9 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IL
-3 protein levels. Biotinylated BVD3-1F9 antibody (PN 731754) should be paired with unlabeled BVD8-3G11 antibody
(PN 731752) as the capture antibody. It is recommended to use purified recombinant human IL-3 as standard.
Immunofluorescence / Flow Cytometry: BVD3-1F9 forms are useful for intracytoplasmic staining and flow cytometric
analysis to identify and enumerate IL-3-positive cells within mixed cell populations.
E C
Clone: BVD8-3G11 Isotype: IgG1 Rat
Western Blotting: the unlabeled BVD8-3G11 antibody is useful for Western blotting applications in conjunction with AP-
labeled Goat Anti-Rat Ig, PN 732647.
E W
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Human non CDs
IL-4
Interleukin-4 (IL-4) is a monomeric 20 kDa predominantly produced by activated T lymphocytes of the Th2 phenotype, by mast
cells and by some antigen-presenting cells. Together with IL-2, IL-4 is an autocrine growth factor for activated T cells and for
cytotoxic T cell precursors. IL-4 is a major factor involved in the activation of resting B lymphocytes. Together with IL-13, IL-4
induces isotype switch towards IgE and IgG1 isotypes. Activated CD4
+
T cells of the Th1 phenotype produce predominantly IL
-2 and Interferon , whereas CD4
+
T cells of the Th2 phenotype produce IL-4, IL-5, IL6 and IL-10.
The 4D9-PE-conjugated antibody is optimized for flow cytometric detection of intracellular IL-4 in stimulated peripheral
mononuclear cells (PBMC) after permeabilizing with formaldehyde / saponin-based IntraPrep Permeabilization Reagent.
C Ci
Clone: MP4-25D2 Isotype: IgG1 Rat
ELISA: MP4-25D2 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IL
-4 protein levels. Biotinylated MP4-25D2 antibody (PN 731756) should be paired with a suitable unlabeled antibody as
the capture antibody. It is recommended to use purified recombinant human IL-4 as standard. Immunofluorescence /
Flow Cytometry: MP4-25D2 forms are useful for intracytoplasmic staining and flow cytometric analysis to identify and
enumerate IL-4-positive cells within mixed cell populations. Western Blotting: the unlabeled MP4-25D2 antibody is
useful for Western blotting applications in conjunction with AP-labeled Goat Anti-Rat Ig, PN 732647.
C E W
216
Human non CDs
IL-5
Human Interleukin-5 (IL-5) is a 40-45 (dimer) kDa glycoprotein produced by T cells, mast cells and eosinophils that is also
known as eosinophil differentiation factor (EDF) and T cell replacing factor (TRF). IL-5 simulates the differentiation and
activation of eosinophils and induces the production of basophils.
Clone: JES1-39D10 Isotype: IgG2a Rat
ELISA: JES1-39D10 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human
IL-5 protein levels. Biotinylated JES1-39D10 (PN731759) may be paired with unlabeled JES1-5A10 (PN 731757) as
the capture antibody. It is recommended to use purified recombinant human IL-5 as standard. Immunofluorescence /
Flow Cytometry: JES1-39D10 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify
and enumerate IL-5-positive cells within mixed cell populations. Western Blotting: the unlabeled JES1-39D10 antibody
is useful for Western blotting applications in conjunction with AP-labeled Goat Anti-Rat Ig, PN 732647.
E C W
Clone: JES1-5A10 Isotype: IgG2a Rat
E
IL-5R
This monoclonal antibody detects the chain of the IL-5 receptor (IL-5R). The chain
associates with a chain (CD131) to form the high affinity IL-5 receptor. This chain is
shared with the IL-3 and GM-CSF receptors. In contrast to the related IL-3 and GM-CSF
receptor chains, IL-5R is eosinophil and basophil specific. Eosinophil and basophil
precursors express IL-5R. Neutrophils, monocytes and lymphocytes do not express IL
-5R. IL-5, with IL-3 and GM-CSF cytokines, are involved in recruiting and acting on
eosinophils and basophils, the major cell populations implicated in allergy, parasitic
infections and asthma. Asthma is characterized by airway inflammation and is associated
with tissue eosinophilia in the lung through chemotactic and cell adhesion mechanisms.
Inflammation is a beneficial key element of the innate immune system in response to
infection or local injury or trauma. But when its normal regulatory mechanisms are
disturbed, chronic inflammatory diseases appear. It has been proposed that cytokine
stimulation of eosinophil survival in the airways of asthmatics is a contributing factor of the
pathology of the disease. A better understanding of the signaling component involved in
eosinophil survival may provide new targets for the treatment of asthma.
Clone: H17N Isotype: IgG1 Mouse
The H17N monoclonal antibody does not block the binding of IL-5 to its receptor.
C B
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Human non CDs
IL-6
Human Interleukin-6 (IL-6) is a 21-26 kDa multifunctional cytokine that is secreted by both lymphoid and nonlymphoid cells
including mononuclear phagocytes, endothelial cells and fibroblasts. IL-6 stimulates the growth of hematopoietic colonies
composed of granulocytes and macrophages, acts as a growth factor for mature B cells, is involved in the activation and
differentiation of T cells and participates in the induction of IL-2 and IL-2 receptor expression.
Clone: MQ2-39C3 Isotype: IgG2a Rat
ELISA: MQ2-39C3 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IL
-6 protein levels. Biotinylated MQ2-39C3 antibody (PN731768) should be paired with unlabeled MQ2-13A5 antibody
Western Blotting: the unlabeled MQ2-39C3 and MQ2-15A5 antibodies are useful for immunoprecipitation and Western
blotting applications.
E W I
Clone: MQ2-13A5 Isotype: IgG1 Rat
Immunofluorescence / Flow Cytometry: MQ2-13A5 antibody is useful for intracytoplasmic staining and flow cytometric
C E I W
IL-7
Human Interleukin-7 (IL-7) is a 20-28 kDa glycoprotein that is dervived from spleen, bone marrow and thymic stromal cells. The
primary biological activity of IL-7 is as a growth factor for progenitor B cells and T cells. IL-7 has also been shown to stimulate
mature T cells and promote the production of lytically active antigen-specific cytotoxic T cells and lymphokine activated killer
(LAK) cells.
Clone: BVD10-11C10 Isotype: IgG2a Rat
ELISA: BVD10-11C10 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying
human IL-7 protein levels. Biotinylated BVD10-11C10 antibody (PN 731771) should be paired with unlabeled BVD10
-40F6 antibody (PN 731769) as the capture antibody. It is recommended to use purified recombinant human IL-7 as
standard. Western Blotting: the unlabeled BVD10-11C10 antibody is useful for immunoprecipitation and Western
blotting applications in conjunction with AP-labeled Goat Anti-Rat Ig, PN 732647.
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Clone: BVD10-40F6 Isotype: IgG1 Rat
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Human non CDs
IL-10
Human Interleukin-10 (IL-10) is an 18 kDa cytokine that is also known as the cytokine synthesis inhibitory factor (CSIF) due to
its role in blocking the activation of cytokine synthesis by Th1 T cells, activated monocytes and NK cells. IL-10 is produced by
a variety of cell types including Th0 and Th2 subsets of CD4
+
T cells and activated B cells. IL-10 has also been shown to
stimulate the proliferation of B cells, thymocytes and mast cells. Viral IL-10 inhibits cytokine production and stimulates B cell
growth similar to its human IL-10 counterpart.
Clone: JES3-12G8 Isotype: IgG2a Rat
ELISA: JES3-12G8 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IL
-10 protein levels. Biotinylated JES3-12G8 antibody (PN 731745) should be paired with unlabeled JES3-9D7 antibody
Western Blotting: the unlabeled JES3-12G8 and JES3-9D7 antibodies are useful for Western blotting applications in
conjunction with AP-labeled Goat Anti-Rat Ig, PN 732647.
E W
Clone: JES3-9D7 Isotype: IgG1 Rat
Immunofluorescence / Flow Cytometry: the JES3-9D7 antibody is useful for intracytoplasmic staining and flow
cytometric analysis to identify and enumerate IL-10-positive cells within mixed cell populations.
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`
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Human non CDs
IL-13
Human Interleukin-13 (IL-13) is a 9-12 kDa IL-4-related cytokine that is produced by activated T cells, mast cells and NK cells.
IL-13 regulates B cells and monocyte function.
Clone: JES10-2E10 Isotype: IgG1 Rat
ELISA: JES10-2E10 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human
IL-13 protein levels. Biotinylated JES10-2E10 antibody (PN 731775) should be paired with unlabeled JES10-35G12
antibody (PN 731772) or JES10-5A2 (PN 731773) as the capture antibody. It is recommended to use purified
recombinant human IL-13 as standard. Western Blotting: the purified JES10-2E10 and JES10-5A2 antibodies are
useful for Western blotting applications in conjunction with AP-labeled Goat Anti-Rat Ig, PN 732647).
Clone: JES10-35G12 Isotype: IgG2a Rat
E
Clone: JES10-5A2 Isotype: IgG1 Rat
Immunofluorescence / Flow Cytometry: JES10-5A2 antibody is useful for intracytoplasmic staining and flow cytometric
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Human non CDs
Chain
The Kappa light chain of the cell surface immunoglobulins is expressed on a subset of 50% of normal human peripheral mature
B lymphocytes and on a subset of bone marrow immature B cells.
Clone: Polyclonal Isotype: IgG Rabbit
C
E
Clone: KP-53 Isotype: IgG1 Mouse
The KP-53 antibody recognizes light chains in free and intact immunoglobulin molecules. Tissue reactivity: KP-53
reacts with B cells of the follicle center, mantle cells and plasma cells. Staining pattern: membrane and cytoplasm.
Ki-67
Ki-67 nuclear antigen is associated with cell proliferation and is found throughout the cell cycle (G1, S, G2, M phases). It is
never expressed by resting (G0) cells.
Clone: Ki-67 Isotype: IgG1 Mouse
The monoclonal Ki-67 antibody reacts with a nuclear antigen associated with cell proliferation and found throughout
the cell cycle (G1, S, G2, M phases) and absent in resting (G0) cells. Studies have shown that the monoclonal antibody
Ki-67 is useful in establishing the cell proliferating fraction in neoplasms (immunohistochemically quantified by
determining the number of Ki-67-positive cells among the total number of resting cells = Ki-67 index). In neoplastic
tissues, the prognostic value is comparable to the tritiated thymidine-labeling index. Studies have shown that the
correlation between low Ki-67 index and histologically low-grade tumors is strong.
F W P C Ci
- Liquid RUO IM1316 2 mL Supernatant
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Human non CDs
KOR-SA3544
The KOR-SA-3544 antigen is a granulocyte cell surface protein of 80-100 kDa.
Clone: KOR-SA3544 Isotype: IgG1 Mouse
In peripheral blood, the KOR-SA3544 antibody reacts with the majority of granulocytes but not with lymphocytes,
monocytes, platelets or red blood cells. In normal bone marrow, only the myeloid cells are positive.
C W P
- Liquid ASR IM2039U 0.5 mL FITC
- Liquid ASR IM2357U 0.5 mL PE
Lactoferrin
Lactoferrin is a 76 kDa single chain polypeptide with iron-binding property. This protein
shows structural similarity to the plasma iron-transport protein transferrin. Lactoferrin is
involved in various immuno-regulatory functions associated with anti-infective and
inflammatory responses such as antibody synthesis, cytokine production, NK cell
cytotoxicity, complement activation and lymphocyte proliferation. Lactoferrin synthesis
occurs at the myelocytic stage during polymorphonuclear neutrophil (PMN) ontogeny and
constitutes a component of secondary (or specific) granules. Unlike the myeloperoxydase,
lactoferrin is absent from primary (or azurophilic) granules and appears later during
maturation of the granulocytic lineage. Thus, in peripheral blood, intracellular lactoferrin
expression is restricted to neutrophils.
Clone: CLB13.17 Isotype: IgG1 Mouse
The PE-conjugated CLB-13.17 antibody is optimized for flow cytometric detection of intracellular lactoferrin by flow
cytometry after permeabilization with IntraPrep permeabilization reagent.
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Human non CDs
Chain
The Lambda light chain of immunoglobulins is expressed at the surface of a cellular sub-
population corresponding to approximately 1/3 of mature B lymphocytes in peripheral
blood. This light chain is also found on the surface of a sub-population of immature bone
marrow B lymphocytes.
Plasma cells in lymph node (DAB).
C
Clone: HP-6054 Isotype: IgG2a Mouse
Clone: JDC-12 Isotype: IgG1 Mouse
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E
Laminin
Clone: 2D11 Isotype: IgM Mouse
2D11 reacts with Human Laminin. Cross reactivity with other species has not been established.
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Cell Lab Liquid RUO A55729 0.1 mg FITC
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Human non CDs
Melanoma cells (membrane ag)
Clone: KBA62 Isotype: IgG2a Mouse
KBA62 recognizes an intracellular membrane antigen. Tissue reactivity: KBA62 does not react with basal melanocytes
in the epidermis, but occasionally stains endothelial cells. Staining pattern: membrane with little or no cytoplasmic
reactivity.
P W F P
MMP-1 (Collagenase-1)
Matrix Metalloproteinases (MMPs) are a family of at least 20 structurally related, zinc-containing enzymes that have the ability to
breakdown connective tissue. MMP-1 is also known as collagenase, fibroblast collagenase, and interstitial collagenase. MMP
-1 has shown substrate specificity to collagens I, II, III, VII, VIII, and X, aggrecan, versican, tenascin, casein, gelatin, 1-
antitrypsin, myelin basic protein, L-selectin, pro-TNF, IL-1 , pro-MMP-2, and pro-MMP-9 directly and indirectly by activating
other MMPs.
Clone: SB12e Isotype: IgG2b Mouse
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MMP-2 (Type IV collagenase)
breakdown connective tissue. MMP-2 is also known as gelatinase A, 72 kDa gelatinase, type IV collagenase and TBE-1. MMP
-2 along with MMP-9 belongs to the gelatinase group of MMPs which have three repeats of a type II fibronectin domain
inserted in the catalytic domain. MMP-2 has shown direct and indirect substrate specificity to collagens I, IV, V, VII, X and XIV,
gelatin, elastin, laminin-1, laminin-5, myelin basic protein, MMP-1, MMP-9 and MMP-13.
Clone: SB13a Isotype: IgG1 Mouse
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MMP-3 (Stromelysin-1)
breakdown connective tissue. MMP-3 is also known as stromelysin-1 and transin, belongs to the stromelysin group of MMPs
which also includes MMP-10. MMP-3 has shown direct and indirect sustrate specificity to collagens III, IV, V, and IX, gelatins,
fibronectin, fibrinogen, plasminogen, proteoglycans, elastin, casein, entactin, myelin basic protein, IL-1 , serum amyloid A. TIMP
-2, MMP-1, MMP-7, MMP-8, MMP-9, and MMP-13.
Clone: SB14d Isotype: IgG2a Mouse
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Human non CDs
MMP-9 (Gelatinase B)
Matrix metalloproteinases (MMPs) are a family of at least 20 structurally related, zinc-containing enzymes that have the ability to
breakdown connective tissue. MMP-9 is also known as gelatinase B and 92 kDa gelatinase. MMP-9, along with MMP-2,
belongs to the gelatinase group of MMPs which have three repeats of a type II fibronectin domain inserted in the catalytic
domain. MMP-9 has shown direct and indirect substrate specificity to collagens IV, V, X and XIV, gelatin, elastin,
proteoglycans, plasminogen, aggrecan, galectin-3, entactin, fibrillin, fibrin, IL-1 , amyloid peptide and myelin basic protein.
Clone: SB15c Isotype: IgG2a Mouse
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Myeloperoxidase
Myeloperoxidase (MPO) is an 22 heteromultimer with and subunits of 55 and 15
kDa respectively. It is expressed intracellularly in the azurophilic granules of neutrophils. It
is not expressed in lymphocytes, megakaryocytes, or in erythrocytes.
Clone: CLB-MPO-1 Isotype: IgG2a Mouse
The CLB-MPO-1 antibody recognizes MPO and its enzymatically inactive form. This antibody can be used to detect
intracytoplasmic myeloperoxidase in flow cytometry after permeabilization with IntraPrep permeabilization reagent.
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Human non CDs
NKp80
The NKp80, also known as killer cell lectin-like subfamily F, member 1 (KLRF1) is a type II
transmembrane glycoprotein of 80 kDa and 40 kDa under reducing conditions. It is
expressed on NK cells and a subset of T lymphocytes whereas it is not expressed on B
lymphocytes, monocytes and granulocytes. The NKp80 antigen is a 231 amino acid
protein which belongs to the C-lectin family and is encoded by a gene located on
chromosome 12. It is characterized, in its 169 amino acid extracellular domain by 4
glycosylation sites and by two tyrosine residues in its 39 amino acid cytoplasmic domain.
The transmembrane domain contains 23 amino acids. The NKp80 molecule binds to the
genetically linked receptor AICL which is up-regulated by Toll-like receptor stimulation.
NKp80-AICL interaction regulates the immune responses at sites of inflammation by
stimulating the release of proinflammatory cytokines.
Anti-NKp80-PE versus CD56-PC5, gated
on C1 region, shows NKp80 expression on
NK cells.
Clone: MA152 Isotype: IgG1 Mouse
The monoclonal antibody MA152 recognizes NK cells, a subset of T lymphocytes. It does not react with NK92, NK3.3
and YT cell lines.
C
NTB-A
The natural killer (NK), T lymphocyte, B lymphocyte antigen (NTB-A) is a 60-kDa
transmembrane glycoprotein of the CD2 family expressed on all human resting and
activated NK, T and B cells. This antigen is not expressed on monocytes, neutrophils, and
basophils but is expressed on eosinophils. The NTB-A antigen, a 331 amino acid protein,
is a member of the immunoglobulin (Ig) superfamily. It is characterized, in its 225 amino
acid extracellular domain, by one distal V-type domain without disulfide bond and two
proximal C2-type domains, and in its 83 amino acid cytoplasmic domain by three tyrosine
residues. One tyrosine residue is part of an ITIM motif and two are parts of an ITSM motif
(TxYxxV/I). NTB-A is its own ligand through an homophilic binding. NTB-A associates with
two adaptor proteins, the SLAMassociated protein (SAP) also known as SH2D1A and
the Ewings sarcoma activated transcript (EAT) 2. In NK cells, NTB-A acts as an activating
coreceptor.
Anti-NTB-APE versus CD3FITC shows
NTB-A expression on NK cells.
Clone: MA127 Isotype: IgG1 Mouse
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Human non CDs
OSCAR
OSCAR, an abbreviation of osteoclast-associated receptor, is a new member of the
leukocyte receptor complex. Human OSCAR is a cell-surface, monomeric N-glycosylated
protein of approximately 45 kDa. The amino acid sequence shows homology with that of
members of the KIR family. The protein exhibits two C2-type immunoglobulin extracellular
domains, a transmembrane section that contains a positively charged arginine residue, and
a short intracytoplasmic tail with no recognizable signaling motif. As suggested by the
presence of the charged residue, OSCAR associates with the ITAM-adapter FcR and not
with other signaling molecules. The gene coding for OSCAR has been mapped to
chromosome 19q13. Unlike mouse OSCAR, human OSCAR is widely expressed in cells
of the myeloid lineage, and in particular, at all stages of dendritic cell (DC) differentiation
and maturation. T cells, B cells, and NK cells do not express OSCAR. OSCAR is involved
in antigen presentation and activation of human DCs. It allows processing and
presentation of exogenous antigens.
Clone: 11.1CN5 Isotype: IgG1 Mouse
C
p53 Protein
p53 is a nuclear phosphoprotein involved in the regulation of the cell cycle. The p53 gene
is one of the most mutated genes in transformed cells, leading to protein conformational
changes and accumulation of very stable mutant forms of the p53 protein in the nucleus.
High-grade B-cell pathology (DAB)
Clone: DO-1 Isotype: IgG2a Mouse
The DO-1 antibody reacts with both wild-type and mutant p53 proteins. Tissue reactivity: DO-1 is the most suitable
antibody for immunohistochemistry detection of p53. DO-1 reacts with the nucleus of some very rare cells in various
non-transformed tissues. Staining pattern: nucleus.
P
227
Human non CDs
p75 AIRM1 (CDw328)
The p75 adhesion inhibitory receptor molecule 1 (p75/AIRM1), also known as Siglec-7,
belongs to the CD33-related Siglec subgroup. p75 AIRM1 is a type I transmembrane
glycoprotein, belonging to the immunoglobulin superfamily (Ig-SF) and sharing sequence
homology with the sialic acids binding Ig-like lectins. The extracellular domain of the
p75/AIRM1 molecule is relatively highly N-glycosylated and displays three extracellular Ig-
like domains. The cytoplasmic tail is characterized by a classical ITIM motif responsible for
the inhibitory functions of this receptor. p75 AIRM1 is expressed on activated and resting
NK cells, monocytes, some chronic (CML) and acute myeloid leukemias (AML). p75
AIRM1 is a novel, HLA independent, inhibitory receptor that may play a relevant role in the
negative regulation of human NK cell functions.
Clone: Z176 Isotype: IgG2b Mouse
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Perforin
Perforin is a 70 kDa cytolytic pore-forming protein that is expressed in cytotoxic lymphocytes (CTLs)
and natural killer (NK) cells. It is located in the cytoplasm and is associated with granules. Perforin and Fas ligand are the key
effectors in T cell-mediated cytotoxicity which mediates cytolysis of target cell by membrane damage and apoptosis. Perforin
plays an important role in the immune response against tumors, allograft rejections and virus infections.
Clone: 4E4 Isotype: IgG2a Mouse
C E
Phosphotyrosine
Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g., via growth factors) during
normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine
phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and
characterization of proteins containing phosphotyrosine.
Clone: PY20 Isotype: IgG2b Mouse
Py20 mAb prevents internalization of activated receptors (e.g., EGFR) when microinjected into cells. The affinity of
Py20 for phosphotyrosine is approximately 10
6
-10
7
M
-1
.
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Human non CDs
Progesterone Receptor
Clone: PR10A9 Isotype: IgG2a Mouse
The PR10A9 antibody recognizes the C-terminal domain of the progesterone receptor. Tissue reactivity: both
antibodies react with cells from the uterus, stromal, epithelial and smooth cells of the endometrium, as well as normal
and hyperplasic cells from ductal and lobular epithelium of mammary glands. Staining pattern: PR10A9 stains nucleus,
occasionally cytoplasm. 1A6 stains nucleus.
F P
The 1A6 antibody recognizes the A/B region of the progesterone receptor.
P P
Proliferation Cell Nuclear Antigen (PCNA)
Proliferation Cell Nuclear Antigen (PCNA), formerly called cyclin, is a 36 kDa proliferation-associated antigen. It is an
intracellular polypeptide antigen that is found in both normal and transformed cells. The level of PCNA synthesis correlates
directly with rates of cellular proliferation and DNA synthesis during the cell cycle. PCNA appears in the nucleus during late G1
phase immediately prior to the onset of DNA synthesis, becomes maximal during S phase and declines again during G2 and M
phases. PCNA is identified as the auxiliary protein to DNA polymerase and is tightly associated with the sites of DNA
replication.
F P W
SMAC (DIABLO)
Smac/DIABLO (second mitochondria-derived activator of caspase/direct IAP-binding protein with low PI) is a mitochondrial
protein that functions as a regulatory component during apoptosis. It is encoded by a nuclear gene and is subsequently
imported into the mitochondria where the N-terminus is removed generating the mature form of the molecule. Smac/DIABLO
has been shown to neutralize the anti-apoptotic effect of the inhibitor of apoptosis protein (IAP) family, a major group of
apoptosis regulators that have been shown to directly block caspase activity thereby blocking apoptosis.
Clone: 2D12 Isotype: IgG2b Mouse
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Human non CDs
TCR PAN /
Clone: IP26A Isotype: IgG1 Mouse
The IP26A antibody recognizes a monomorphic determinant of the human / chain of the TcR complex.
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TCR PAN /
Clone: IMMU510 Isotype: IgG1 Mouse
The IMMU510 monoclonal antibody recognizes all / T cells, regardless of their variable chains. It stains 1.6% to
8.9% of peripheral CD3
+
cells from healthy donors. In Western blot, it recognizes the chain in reducing conditions. It
immunoprecipitates the three isoforms of the / T cell receptor. This antibody is suitable for immunohistochemistry
on frozen sections. It has been used to study the distribution of / T cells and their functions.
C I E F
IOTest Liquid ASR IM2662U 0.5 mL PC5
TCR V24
The C15 antibody recognizes the IGRa02 sequence, the only described member of this family. It stains from 0.4% to
1% of peripheral CD3
+
cells from normal blood. An invariant V24/V11 T cell receptor is expressed in all individuals
by clonally expanded CD4
CD8
T cells, reactive to bacterial antigens. This unique lymphocyte population restricted by

the CD1d molecule recognition has been identified as the natural killer T (NKT) cells. The IGRa02 sequence is also
referred to as TRAV10 (based on the IMGT gene nomenclature).
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Human non CDs
TCR V1
Also called TCRBV1S1 and S2
Clone: BL37.2 Isotype: IgG1 Rat
V1 is the human variable 1 chain of the T cell receptor. The BL37.2 antibody recognizes both V1.1 and V1.2 allele
products (HBVT73 cDNA and 46W/Q cDNA, respectively). None of the other V specific antibodies from the TCR
Workshop (San Francisco, CA, 1995) gave significant staining on the sorted cell lines. This antibody stains
appoximately 6% of peripheral CD3
+
cells in normal blood. This sequence is also referred to as TRBV9 (based on the
IMGT gene nomenclature).
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TCR V2
Also called TCRBV2S1
Clone: MPB2D5 Isotype: IgG1 Mouse
V2 is the human variable 2 chain of the T cell receptor. The MPB2D5 antibody recognizes all the members (Molt4
identical to PL2.13, PH34 and TCRVBV2S13 sequences) of the V2 subfamily that are in fact alleles. It stains
appoximately 10% of peripheral CD3
+
cells in normal blood. V2 is the target of Toxic Shock Syndrome Toxin 1
(TSST1). This sequence is also referred to as TRBV20-1 (based on the IMGT gene nomenclature).
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TCR V3
Clone: CH92 Isotype: IgM Mouse
V3 is the human variable 3 chain of the T cell receptor. The CH92 antibody recognizes the unique member of the
V3 subfamily (PL4.4 identical to HBVT22 sequences). CH92 antibody recognizes the major subset of V3 cells. V3 is
the target of Staphylococcal Enterotoxin B (SEB) superantigen. This sequence is also referred to as TRBV28 (based on
the IMGT gene nomenclature).
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Human non CDs
TCR V4
Clone: WJF24 Isotype: IgM Rat
The WJF24 antibody recognizes the V allele product. This antibody stains from 0.8% to 3.3% (mean 1.9%) of
peripheral CD3-positive lymphocytes in normal blood. This sequence is also referred to as TRBV29-1 (based on the
C
IOTest Liquid ASR B07084 1 mL FITC
TCR V5.1
Clone: IMMU 157 Isotype: IgG2a Mouse
V5 is a multimembered subfamily of the T cell receptor. Immu157 recognizes the V5.1 (HBP51 sequence) member
of this subfamily. V5.2 and V5.3 (PL25 and 12A1 sequences) are not recognized. Recognition of other members of
the V5 subfamily has not been found, but cannot be formally excluded. This antibody stains from 4.2% to 7.2% of
peripheral CD3
+
cells in normal blood. This sequence is also referred to as TRBV5-1 (based on the IMGT gene
nomenclature).
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TCR V5.2
The 36213 antibody recognizes the PL25 sequence. V5.1 (HBP51 sequence) and V5.3 (IGRb08 sequence) are not
recognized. Recognition of other members of the V5 subfamily has not been found, but cannot be formally excluded.
36213 stains from 0.5% to 1.5% (mean 0.8%) of peripheral CD3
+
cells in normal blood. This sequence is also referred
to as TRBV5-6 (based on the IMGT gene nomenclature).
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TCR V5.3
The 3D11 antibody recognizes the IGRb08 sequence. V5.1 and V5.2 (HPB51 and PL25 sequences) are not
recognized. Reactivity on other members of the V5 subfamily has not been found, but cannot be formally excluded. It
stains from 1% to 1.8% (mean 1.4%) of peripheral CD3
+
cells in normal blood. This sequence is also referred to as
TRBV5-5 (based on the IMGT gene nomenclature).
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TCR V7.1
Clone: ZOE Isotype: IgG2a Mouse
The ZOE antibody recognizes V7.1 (PL4.9 sequence). It does not react with V7.2, but the reactivity on other V7
members like V7.3 (HT267.2 sequence) has not been found, but cannot be formally excluded. This antibody stains
from 1.5% to 2.4% of CD3
+
cells in normal blood. This sequence is also referred to as TRBV4-1, TRBV4-2, TRBV4-3
(based on the IMGT gene nomenclature).
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TCR V7.2
Clone: ZIZOU4 Isotype: IgG2a Mouse
The ZIZOU4 antibody recognizes the human TCR V7.2 allele product. A genetically determined insertion/deletion-
related polymorphism of the TCRB locus, including the functional V7.2 variable gene segment, has been described.
Thus, the expression of the V7.2 allele product may not be expressed in some individuals (V7.2 deleted/V7.2
deleted haplotypes). The antibody stains from 0% (V7.2 del./V7.2 del. haplotypes) to 5.5% (mean 1.5%) of
peripheral CD3-positive lymphocytes in normal blood.
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IOTest Liquid ASR B06666 1 mL FITC
233
Human non CDs
TCR V8
Clone: 56C5.2 Isotype: IgG2a Mouse
The 56C5.2 antibody recognizes V8.1 and V8.2 (YT35 and PL3.3 sequences). Other V8 sequences are believed to
be pseudogenes. It stains 2.6% to 5.1% of peripheral CD3
+
cells in normal blood. V8 is the target of Staphylococcal
Enterotoxin E (SEE). This sequence is also referred to as TRBV12-3, TRBV12-4 (based on the IMGT gene
nomenclature).
C F
TCR V9
Clone: FIN9 Isotype: IgG2a Mouse
There is only one functional member of this T cell receptor subfamily (IGRb20 sequence, identical to sequence PL2.6).
The FIN9 antibody recognizes the product of that gene. It stains 1.6% to 3.2% of peripheral CD3
+
cells in normal
blood. This sequence is also referred to as TRBV3-1 (based on the IMGT gene nomenclature).
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TCR V11
Clone: C21 Isotype: IgG2a Mouse
Two V11 sequences (PL3.12, and PH15 sequences) are described in the literature differing only in their leader
sequence, and, therefore, result in the same mature protein. The C21 antibody recognizes the gene product of these
sequences and stains 0.4% to 0.9% of peripheral CD3 positive cells in normal blood. An invariant V24/V11 T cell
receptor is expressed in all individuals by clonally expanded CD4
-
CD8
-
T cells, reactive to bacterial antigens. This
unique lymphocyte population restricted by the CD1d molecule recognition has been identified as the natural killer T
(NKT) cells. The V11 sequence is also referred to as TRV25-1 (based on the IMGT gene nomenclature).
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Human non CDs
TCR V12
Clone: VER2.32.1 Isotype: IgG2a Mouse
The VER2.32.1 monoclonal antibody recognizes HBP54 identical to PH27 sequence often referred to as V12.2. The
reactivity of the other members of this family (PL4.2 sequence) and other recently described V12 genes (HT96
sequence) has not been found but cannot be formally excluded. This antibody stains from 1.1% to 1.9% of peripheral
CD3
+
cells in normal blood. This sequence is also referred to as TRBV10-3 (based on the IMGT gene nomenclature).
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TCR V13.1
Clone: IMMU 222 Isotype: IgG2b Mouse
V13 is a complex subfamily of the T cell receptor. The IMMU 222 antibody recognizes V13.1 member (HBP34
sequence). V13.3 (IGRb14 sequence) is not recognized. Reactivity with other members of this subfamily has not been
found, but cannot be formally excluded. It has been recently shown that Immu 222 can recognize the V13.4 and
V13.6 (IGRb16 sequence) subsets. This antibody stains 1.8% to 3.3% of peripheral CD3
+
cells in normal blood. This
sequence is also referred to as TRBV6-5, TRBV6-6, TRBV6-9 (based on the IMGT gene nomenclature).
C F
TCR V13.2
Clone: H132 Isotype: IgG1 Mouse
The antibody H132 recognizes the V13.2 allele product, but does not react with the V3.1, 13.3, 13.5 and 13.6 allele
products of the V13 subfamily. The specificity of this antibody has been confirmed at the Human TCR Workshop in
San Francisco, CA, in 1995. This antibody stains from 0.8% to 5.3% (mean 2.8%) of peripheral CD3-positive
lymphocytes in normal blood.
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Human non CDs
TCR V13.6
Clone: JU74.3 Isotype: IgG1 Mouse
The JU74.3 antibody recognizes the V13.6 sequence (IGRb16 sequence). V13.1 (HBP34 sequence) is not recognized.
The reactivity of the other members of this complex subfamily has not been found, but cannot be formally excluded.
JU74.3 stains from 0.9% to 1.9% of peripheral CD3
+
cells in normal blood. This sequence is also referred to as TRBV6
-6 (based on the IMGT gene nomenclature).
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IOTest Liquid ASR B07225 1 mL PE
TCR V14
Clone: CAS1.1.3 Isotype: IgG1 Mouse
V14 is a single member subfamily (PL8.1 sequence identical to PH21 sequence). The CAS1.1.3 antibody stains from
2.2% to 5.6% of peripheral CD3
+
cells in normal blood. This sequence is also referred to as TRBV27 (based on the
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TCR V16
Clone: TAMAYA1.2 Isotype: IgG1 Mouse
V16 is a single member subfamily (HBP42 sequence identical to HT370 sequence). The TAMAYA1.2 antibody stains
from 0.4% to 1.1% of peripheral CD3
+
cells in normal blood. This sequence is also referred to as TRBV14 (based on
the IMGT gene nomenclature).
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Human non CDs
TCR V17
Clone: E17.5F3.15.13 Isotype: IgG1 Mouse
The E17.5F3.15.13 antibody recognizes the HBVT02 sequence and is the only member of this subfamily. It stains from
3.3% to 7% of peripheral CD3
+
cells in normal blood. V17 is the target of Mycoplasma Arthritis Superantigen (MAS)
and Staphylococcal Enterotoxin B (SEB). This sequence is also referred to as TRBV19 (based on the IMGT gene
nomenclature).
C F
TCR V18
Clone: BA62.6 Isotype: IgG1 Mouse
The BA62 antibody recognizes the PH29 sequence identical to the HBVT56 sequence, the only member of the V18
subfamily. The BA62 antibody stains from 0.7% to 1.4% of peripheral CD3
+
cells in normal blood. This sequence is
also referred to as TRBV18 (based on the IMGT gene nomenclature).
C F
TCR V20
Clone: ELL1.4 Isotype: IgG2a Mouse
V20 is a single member subfamily (HUT102 sequence) of the T cell receptor. Two alleles have been identified for this
sequence. One of them is a frequent null allele (frequency estimated at 11%). No cells are stained on PBL from certain
specimens wheareas around 2.4% is expected for specimens expressing the functional allele. This sequence is also
referred to as TRBV30 (based on the IMGT gene nomenclature).
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237
Human non CDs
TCR V21.3
Clone: IG125 Isotype: IgG2a Mouse
V21 is a subfamily of the T cell receptor. The IG125 antibody recognizes the IGRb01 sequence identical to TCR
BV21.3 sequence. Recognition of other sequences within this subfamily has not been found, but cannot be formally
excluded. The IG125 antibody stains from 2.2% to 3.6% of peripheral CD3
+
cells in normal blood. This sequence is
also referred to as TRBV11-2 (based on the IMGT gene nomenclature).
C
TCR V22
Clone: IMMU 546 Isotype: IgG1 Mouse
V22 is a subfamily of the T cell receptor. The IMMU 546 antibody recognizes the IGRb03 sequence. Another gene has
been described for this family which differs by only one amino acid and may represent an allelic variant. Recognition of
this sequence is likely, but has not been formally proven yet. The IMMU 546 stains from 2.4% to 5.1% of peripheral
CD3
+
cells in normal blood. This sequence is also referred to as TRBV2 (based on the IMGT gene nomenclature).
C F
TCR V23
Clone: AF23 Isotype: IgG1 Mouse
V23 is a family with a single member (IGRb04 sequence), the product of which is recognized by the AF23 antibody.
V23 is expressed on the HUT78 and H9 cell lines, which is frequently used to grow HIV. AF23 stains from 0.4% to 5%
of peripheral CD3
+
cells in normal blood. This sequence is also referred to as TRBV13 (based on the IMGT gene
nomenclature).
C
238
Human non CDs
TCR V1
Clone: R9.12 Isotype: IgG1 Mouse
The R9.12 antibody recognizes the variable 1 segment of the T cell receptor. V1 is rearranged on both C and C
and is expressed in these two cell types. R9.12 reacts only with
+
V1
+
cells and not with
+
V1
+
cells. V1
+
+
cells are the major T cell population at birth and are over expressed in intestinal intraepithelial lymphocytes. This
sequence is also referred to as TRDV1 (based on the IMGT gene nomenclature).
TCR V2
V2 is the human variable 2 gene of the T cell receptor. V2
+
T cells are the main population of the circulating T
cells. The IMMU 389 antibody gives excellent results in immunofluorescence and frozen sections. It also works in
Western blot. It recognizes the complex from the V2
+
T cell receptor (non reducing conditions), and the isolated
chain under reducing conditions. It immunoprecipates the complex of V2
+
T cell clones. This antibody stains 0.5%
to 10% of PBL in normal blood, representing around 70% of the peripheral T cells. V2 is mostly associated with
V9. This sequence is also referred to as TRDV2 (based on the IMGT gene nomenclature).
C
TCR V9
V9 is the only functional member of the group II variable genes of the T cell receptor. The IMMU 360 antibody
stains 0.5% to 10% of PBL in normal blood, representing around 70% of circulating
+
T cells. Therefore, V9 positive
cells are the major population of circulating T cells. V9 is mainly associated with V2. The combination V9-V2
T cell seems to recognize mycobacterial antigens. IMMU 360 may be used in flow cytometry, immunoprecipitation,
Western blot and immunohistochemistry techniques. This sequence is also referred to as TRGV9 (based on the IMGT
gene nomenclature).
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239
Human non CDs
TCR
The subunit is a 16 kDa surface molecule associated with the T cell receptor. Its expression is restricted to T lymphocytes
and NK cells. In T lymphocytes, the subunit is expressed as a disulfide-linked homodimer of 32 kDa or as a disulfide-linked
heterodimer with a 21 kDa subunit designated . Both of these dimeric species are non-covalently associated with the
remaining subunits of the CD3-TCR-antigen receptor complex. In NK cells, the subunit is expressed as a disulfide-linked
homodimer of 32 kDa or as a disulfide-linked heterodimer with a 12 kDa subunit. It is associated with the CD16 molecule
(FcRIII).
Clone: 2H2D9 (TIA-2) Isotype: IgG1 Mouse
The 2H2D9 (TIA-2) antibody recognizes a cytoplasmic epitope of the subunit of the T lymphocyte receptor complex.
It is suitable for immunoprecipitation, immunoblotting, and flow cytometric analysis of permeabilized cells. The TIA-2
antibody was evaluated during the Fifth International Workshop on Human Leukocyte Differentiation Antigens. TIA-2
antibody can be used to detect intracytoplasmic TCR- in flow cytometry after permeabilization with digitonin.
C W I F
TdT
Terminal Deoxynucleotidyl Transferase (TdT) is an intra nuclear marker normally present at high levels in cortical thymocytes
and a minor subset of bone marrow pre-lymphocytes.
Clone: HT1 + HT4 + HT8 + HT9 Isotype: IgG1 + IgG2a Mouse
C
- Liquid CE IM3524 50 tests FITC
- Liquid ASR IM3524U 1 mL FITC
240
Human non CDs
Thrombin receptor
The Thrombin Receptor (60 to 80 kDa) is expressed on a variety of cells including platelets
and endothelium. The receptor is a substrate of thrombin and has been shown to play a
role in the activation of platelets.
HEL cell line. Indirect staining.
Clone: SPAN 12 Isotype: IgG1 Mouse
The SPAN 12 antibody recognizes an epitope on the receptor that spans the thrombin cleavage site. It binds to intact
receptors, but not to cleaved ones. SPAN 12 inhibits thrombin-induced receptor activation at reduced thrombin
concentrations.
C
Clone: WEDE15 Isotype: IgG1 Mouse
The WEDE15 antibody recognizes an epitope on the receptor, distant from the N-terminal thrombin cleavage site
(WEDEE). It reacts with both intact and cleaved receptors.
C E
Thrombospondin
Thrombospondin is a 450 kDa -granule glycoprotein involved in platelet aggregation. It is expressed on the platelet surface
after activation.
Clone: P10 Isotype: IgG1 Mouse
F C
241
Human non CDs
Thyroglobulin
Thyroglobulin is a hormone produced by the follicular cells of the thyroid gland. It is
involved in T3 and T4 thyroid hormone synthesis. It does not cross-react with T4 and T3.
Skin metastasis of an adenocarcinoma of
the thyroid
Clone: Pool Isotype: IgG1 Mouse
The three clones of the pool recognize different epitopes of the thyroglobulin molecule. Tissue reactivity: this antibody
mixture reacts with thyroid follicular epithelial cells and with the colloid. The intensity of the staining is variable. Other
normal tissues are negative. Staining pattern: cytoplasm and diffuse colloid staining of thyroid follicles.
TIA-1
The T cell intracellular antigen 1 (TIA-1) is a 17 kDa cytoplasmic granule-associated protein, expressed in cells possessing
cytolytic potential. TIA-1 is also designated as GMP-17 (granule membrane protein of 17 kDa). The GMP-17/TIA-1 molecule
could be involved in the molecular signaling cascade during Fas (CD95)-mediated apoptosis. In normal peripheral blood
lymphocytes, TIA-1 is expressed by 90% of CD16
+
, 50-60% of CD8
+
, and less than 10% of CD4
+
cells. Although TIA-1 may
be detected in unstimulated T lymphocytes, activated T lymphocytes are induced to express disulfide linked polymers of the 17
kDa protein, also recognized by TIA-1. TIA-1 is expressed by all monocytes and granulocytes, by activated CD4
+
T-cell clones
and activated natural killer cell clones, and by Con A-activated thymocytes. On the contrary, TIA-1 is not expressed by B
lymphocytes or B-cell lines.
Clone: 2G9A10F5 Isotype: IgG1 Mouse
The 2G9 antibody is a useful reagent for detecting cytolytic effector cells in tissue infiltrates. It was evaluated during
HLDA 5 in the section of monoclonal antibodies reactive with intracellular antigens. It may be used to detect
intracytoplasmic TIA-1 in flow cytometry after permeabilization with IntraPrep permeabilization reagent.
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242
Human non CDs
TNF
Tumor necrosis factor (TNF) or cachectin is a cytokine that was first identified based
on its cytotoxic activity against various cell lines and tumor cells. Human TNF is
synthesized as a pro-protein with a molecular weight of 26 kDa (membrane-bound form).
The pro-protein is cleaved by a specific metalloprotease (TACE) yielding a monomeric,
soluble form of 17 kDa. Under physiological conditions, TNF forms a homotrimeric
protein. This cytokine is primarily produced by mononuclear phagocytes, and by activated
lymphocytes. Immunostaining allows intracellular detection of TNF before it can be
detected by a bioassay. TNF acts on target cells by binding to two types of receptors,
the TNF receptor I (TNF-RI or CD120a) and the TNF receptor II (TNF-RII or CD120b).
TNF elicits a wide spectrum of immune and inflammatory responses, including the
induction of other cytokines and immunoregulatory molecules, cell growth and
differentiation and apoptosis. This molecule is also involved in Th1 - Th2 (T helper 1 - T
helper 2) cytokine pathways (or Type 1 - Type 2 responses), as a Th1-effector cytokine.
Activated PBL.
Clone: IPM2 (188) Isotype: IgG1 Mouse
C
Villin
Villin and related proteins gelsolin, fragmin and severin, regulate actin assembly. Villin is
composed of three domains. The first two domains are homologous (gelsolin-like domains)
and the third domain, the "headpiece", is a small, 36-residue alpha helical protein located
at the C-terminus of the protein. This "headpiece" is perhaps one of the smallest, fastest-
folding proteins known. Villin is mainly produced by epithelial cells which develop a "brush
border" such as absorptive cells of the intestine and epithelial cells of the proximal renal
tubes.
Small intestine (AEC)
Clone: ID2C3 Isotype: IgG1 Mouse
The ID2C3 antibody recognizes a cytoskeletal filament of 58 kDa. Tissue reactivity: ID2C3 reacts with brush border
epithelia of the intestine and kidney. Staining pattern: apical or brush border, occasionally cytoplasm.
P F C
243
Human non CDs
Vimentin
Vimentin (58 kDa) is a ubiquitous intermediate filament protein and the first to be
expressed during cell differentiation. All primitive cell types express vimentin, but in most
non-mesenchymal cells other intermediate filament proteins replace it during
differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types:
fibroblasts, endothelial cells etc., as well as in a number of other cell types derived from
mesoderm.
Rhabdomyosarcoma (AEC)
Clone: V9 Isotype: IgG1 Mouse
Tissue reactivity: the V9 antibody reacts with cells of mesenchymal origin: fibroblasts and endothelial cells are stained
by this antibody. Staining pattern: cytoplasm.
F W P E
von Willebrand's factor
von Willebrands factor (vWF) is a glycoprotein that circulates in plasma tightly bound to factor VIII to form the FVIII / vWF
complex. vWF is synthesized in endothelial cells and megakaryocytes. In endothelial cells, it is either stored in intracellular
organelles, known as Weibel-Palade bodies, or secreted constitutively. In megakaryocytes and platelets, it is stored in -
granules. In addition to serving as the carrier protein for factor VIII, vWF acts as an adhesive protein by mediating platelet
adhesion to sub-endothelium through its binding sites for the platelet receptor GPIb-a-IX and collagen. It also aids platelet
platelet interaction through its binding site for platelet GPIIb/IIIa.
The 4F9 antibody is directed towards a human von Willebrand's factor epitope found on endothelial cells.
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244
- Bovine
- Chicken
- Feline
- Porcine
- Rabbit
Systems and Kits ------------------------------------- 246
Multi-Color Reagents --------------------------- 247-248
Single-Color Reagents
- Mouse ---------------------------------- 249-300
- Rat ------------------------------------- 301-303
- Other Species -------------------------- 304-321
245
Systems ans Kits
gTox Flow
Diluent 17.4 mL
Anti-Mouse CD71-FITC (Clone RI7.217) 0.6 mL
Permeabilizing Reagent 24 mL
RNase 24 mL
Propidium Iodide 24 mL
Mouse Peripheral Blood Micronuclei Enumeration Kit for Flow Cytometry
Erythropoieisis that takes place in bone marrow is the differentiation of erythroblasts into erythrocytes. A dramatic
process during this maturation is the expulsion of the nucleus. The erythrocytes released in the peripheral blood are
enucleated making these cells an exception in the cellular process of differentiation. The integrity of the genome can
be altered either by clastogenic compounds (leading to the breakage of double stranded DNA) or by aneugenic
compounds which will directly interfere with the mitotic apparatus. Both mechanisms will lead to the presence of DNA
fragments or an entire chromosome, called micronucleus, in the daughter cells (MNPCE: Micronucleated polychromatic
erythrocytes or MNNCE: Micronucleated normochromatic erythrocytes) which will persist in peripheral whole blood and
is easily detected in enucleated reticulocytes and erythrocytes. Toxicology studies that are performed on animals play
a key role during the drug testing process. As drug candidates may affect genome integrity, they require reliable
methods of determination of affected physiological functions. Flow cytometry can be used to measure the percentage
of micronucleated reticulocytes in peripheral blood as it correlates with genetic damages. Therefore, it provides a
useful assay to assess the status of peripheral reticulocytes during pre-clinical assays.
RUO
Status Part #
60 tests
Size
A49073
246
Optimized IOTest three-color combinations of conjugated monoclonal antibodies that allow the characterization
and enumeration of lymphocyte sub-populations in rat and in non-human primate. They guarantee an easy flow
cytometry multiparametric analysis after red blood cell lysis using a No-Wash procedure. A variety of biological
samples can be analyzed, including whole blood, dissociated spleen cells or dissociated lymph node cells, and
bone marrow.
Non-Human Primate
3 color
Non-Human Primate (NHP) T-Cell Subpopulation iTox3 Combination
Anti-NHP CD3-FITC / CD4-PC7 / CD8-APC combination is designed to
discriminate CD3
+
T cell as well as CD4
+
and CD8
+
T cell subpopulations.
CD3-FITC/CD4-PC7/CD8-APC
CD3-FITC FN18 IgG1 (mouse)
CD4-PC7 M-T477 IgG2a (mouse)
CD8-APC B9.11 IgG1 (mouse)
Non-Human Primate (NHP) T-Cell Subpopulation iTox3 Combination
Anti-NHP CD3-FITC / CD20-PC7 / CD16-APC combination is designed to
discriminate T cells, B cells and NK cells.
CD3-FITC FN18 IgG1 (mouse)
CD20-PC7 B9E9 IgG2a (mouse)
CD16-APC 3G8 IgG1 (mouse)
247
Optimized IOTest three-color combinations of conjugated monoclonal antibodies that allow the characterization
and enumeration of lymphocyte sub-populations in rat and in non-human primate. They guarantee an easy flow
cytometry multiparametric analysis after red blood cell lysis using a No-Wash procedure. A variety of biological
samples can be analyzed, including whole blood, dissociated spleen cells or dissociated lymph node cells, and
bone marrow.
Rat
3 color
Rat T-Cell Subpopulation iTox3 Combination
Anti-rat CD3-FITC / CD4-PC7 / CD8-APC combination is designed to
discriminate CD3
+
T cell as well as CD4
+
and CD8
+
T cell subpopulations.
CD3-FITC 1F4 IgM (mouse)
CD4-PC7 OX-38 IgG2a (mouse)
CD8-APC OX-8 IgG1 (mouse)
Rat T-Cell Subpopulation iTox3 Combination
Anti-Rat CD3-FITC / CD45RA-PC7 / CD161a-APC combination is designed
to discriminate T- , B-, and NK-cell sub-populations.
CD3-FITC/CD45RA-PC7/CD161a-APC
CD3-FITC 1F4 IgM (mouse)
CD45RA-PC7 OX-33 IgG1 (mouse)
CD161a-APC 10/78 IgG1 (mouse)
248
Mouse
Anti-Mouse Antigens
CD2
CD2, also known as leukocyte function-associated antigen-2 (LFA-2), is a member of the
immunoglobulin superfamily of cell adhesion molecules. It is expressed on the surface of >95 % of
thymocytes and essentially all B-cells, T-cells, and NK cells. In the mouse, it is the receptor for
CD48. CD2 is thought to play a major role in mediating T-cell adhesion, T-cell
recognition/activation, T-cell signaling and intrathymic differentiation.
Clone: 12-15 Isotype: IgG1 Rat
This antibody can be used for in vitro inhibition of CTL-mediated lysis.
Cell Lab Liquid LUO 731977 0.1 mg PE
Cell Lab Select Liquid LUO A88546 0.1 mg PC7
CD3
CD3 is a member of the T cell receptor-associated CD3 complex. It is found on a subpopulation
of thymocytes and on all T lymphocytes of all mouse strains.
Clone: 145-2C11 Isotype: IgG Armenian Hamster
The 145-2C11 monoclonal antibody can activate both cloned T cell lines and normal T cells.
Under certain conditions, T cell activation by 145-2C11 may result in apoptotic cell death.
Cell Lab Liquid LUO 731983 0.1 mg APC
Cell Lab Liquid LUO 733253 0.1 mg CY5
249
Mouse
Anti-Mouse Antigens
Clone: 500-A2 Isotype: IgG Syrian Hamster
Clone: C363.29B (YCD3-1) Isotype: IgG2b Rat
The C363.29B MAb can activate both cloned T cell lines and normal T cells provided the
appropriate accessory signals are present. It can be used for in vitro depletion of CD3
+
cells as
well as for in vitro activation of T cells.
Cell Lab Select Liquid LUO A88595 0.1 mg ECD
Cell Lab Select Liquid LUO A88596 0.1 mg PC5.5
Cell Lab Select Liquid LUO A88598 0.1 mg APC-Alexa Fluor* 700
250
Mouse
Anti-Mouse Antigens
CD4 (L3T4a)
CD4/L3T4a is a type I transmembrane glycoprotein expressed on most thymocytes and the
helper/inducer subpopulation of mature T lymphocytes of all mouse strains. CD4 functions as an
accessory molecule in the recognition of foreign antigens in association with MHC Class II antigens
by T cells.
Clone: GK1.5 Isotype: IgG2b Rat (Lewis)
This antibody can be used for in vitro depletion of CD4
+
cells as well as for inhibition of T helper
function or fractionation of CD4
+
cells.
251
Mouse
Anti-Mouse Antigens
CD5 (Lyt-1)
CD5/Lyt-1 is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T
lymphocytes and a subset of B lymphocytes, but not on natural killer cells. The frequency of CD5
+
B cells exhibits strain-dependent variation, and appears to represent a distinct lineage of B
lymphocytes known as B-1 cells. CD5 has been identified as a ligand for the B cell antigen,
CD72/Lyb-2.
Clone: 4H8E6 Isotype: IgG2a Rat
This antibody can be used for activation of T cells in vitro.
Clone: B19.1 Isotype: IgG2a Rat
This antibody can be used for activation of T cells in vitro.
252
Mouse
Anti-Mouse Antigens
CD8 (CD8 / Lyt-2)
Mouse CD8 exists in two forms: (i) a CD8 heterodimer composed of an chain and a chain; and
(ii) a homodimer of two chains. The heterodimer is found on essentially all thymocytes and the
suppressor/cytotoxic subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial
lymphocytes express CD8 without CD8. CD8 acts as a co-receptor with MHC Class I-restricted
T cell receptors in antigen recognition and positive selection of MHC Class I-restricted CD8
+
T
cells.
Clone: 53-6.7 Isotype: IgG2a Rat
This antibody can be used for in vitro depletion of CD8
+
cells, for fractionation of CD8
+
T cells
as well as for blocking of cytotoxicity.
CD11a (LFA-1)
CD11a is the subunit of leukocyte-function associated antigen-1, a member of the integrin family
of cell adhesion molecules. It is expressed on thymocytes, T and B lymphocytes, and on non-
lymphoid cells in the bone marrow. CD11a appears to be an essential component for T cell
activation by antigen-presenting cells and is important for cell-mediated killing. Costimulatory
signaling by LFA-1 is achieved by pairing with its ligand, ICAM-1 (CD54).
Clone: I21/7 Isotype: IgG2a Rat
253
Mouse
Anti-Mouse Antigens
CD11b (Mac-1)
CD11b, also known as complement receptor type 3, represents the integrin M subunit which
combines with the integrin 2 chain (CD18) to form the integrin Mac-1 heterodimer. It is expressed
at varying levels on granulocytes, macrophages, dendritic cells, NK cells and B-1 cells in the
peritoneal and pleural cavities. In addition to binding type 3 complement on opsonized targets, it
mediates the subsequent ingestion process. Mac-1 is also important in transendothelial migration
of monocytes and neutrophils.
Clone: M1/70 Isotype: IgG2b Rat
MAb M1/70 has been observed to cross-react with CD11b on human monocytes,
polymorphonuclear leukocytes, and NK cells. It is suitable for use on acetone-fixed frozen sections
only.
Clone: 3A33 Isotype: IgG2a Rat (Lewis)
254
Mouse
Anti-Mouse Antigens
CD16/32 (FcR)
The lymphocyte Fc receptors recognize the Fc portion of IgG, presented either as immune
complexes or as free antibody. CD32/FcIII is the low affinity receptor and is the most predominant.
It is expressed on a variety of cell types including B cells, monocytes, macrophages and
neutrophils. CD16/FcII receptor is of higher affinity and is expressed on macrophages, neutrophils
and natural killer cells.
Clone: 93 Isotype: IgG2a Rat
MAb 93 recognizes a conformational epitope common to CD32 and CD16/FcII. This antibody
can be used for in vitro blocking of Fc-mediated binding.
CD18
CD18 represents the common integrin 2 chain of the CD11/C18 heterodimer complexes of LFA
-1, Mac-1, and gp150/95. LFA-1 is expressed primarily on leukocytes where it is involved in a
variety of homotypic and heterotypic adhesion functions. Mac-1 is expressed mainly on myeloid and
natural killer cells where it mediates adhesion to CD54/ICAM-1. The gp150/95 heterodimer is
expressed on dendritic cells and CD4
-
CD8
+
IEL and plays a role in binding C3bi.
Clone: C71/16 Isotype: IgG2a Rat (Lewis)
255
Mouse
Anti-Mouse Antigens
CD19
CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels
throughout B cell development from early pro-B/pre-B cells (i.e., B220
+
/CD43
+
/HSA
+
) through
fully differentiated B cell stages. Terminally differentiated plasma cells do not express CD19. In
humans, the CD19 molecule on the surface of mature B cells associates with CD21 and CD81,
and this multimolecular complex synergizes with surface immunoglobulin to provide signal
transduction and promote cellular activation. All splenic and peritoneal IgM
+
cells of both B-1 and B
-2 lineages are CD19
+
, with B-1 cells expressing higher levels of CD19 than B-2 cells in these
sites. Studies with CD19-deficient mice have suggested that this molecule may not be required for
normal generation and maturation of B cells in the bone marrow.
Clone: 6D5 Isotype: IgG2a Rat
This antibody can be used for in vitro functional studies.
Clone: MB19-1 Isotype: IgA Mouse (129 x C57BL/6)
256
Mouse
Anti-Mouse Antigens
CD22
CD22, also known as Lyb-8, is a heterodimeric transmembrane glycoprotein and a member of a
structurally related group of immunoglobulin (Ig) superfamily domain-containing proteins called the
sialoadhesin family. It is detected in the cytoplasm early in B cell development (late pro-B cell
stage), appears on the cell surface concomitant with IgD, and is found on most IgM
+
IgD
+
mature B
lymphocytes. Expression is lost with terminal differentiation of B cells and is absent on plasma cells.
Activation of B cells via cross-linking of surface Ig increases CD22 expression. CD22 associates
with the B cell receptor (BCR) complex and mediates intercellular adhesion. Its intracellular domain
is phosphorylated after antigen receptor cross-linking and is involved in negative regulation of B-cell
activation.
Clone: 2D6 (NIMR-6) Isotype: IgG1 Rat
This antibody can be used for blocking of B cell homotypic adhesion.
CD23
CD23, a type II transmembrane heterodimer, is the low affinity receptor for IgE found on most
mature, conventional B lymphocytes (but not on CD5
+
B cells). It is distinct from the high affinity IgE
receptors on basophils and mast cells. CD23 mediates IgE-dependent cytotoxicity and
phagocytosis by macrophages and eosinophils, and is thought to play a role in isotype-specific
immunoregulation.
Clone: 2G8 Isotype: IgG2a Rat (LOU/M)
257
Mouse
Anti-Mouse Antigens
CD24
CD24, also known as heat stable antigen (HSA) is a phosphatidylinositol-linked cell surface
glycoprotein expressed on pro-B, pre-B and mature B cells. The expression of CD24 decreases
after B cell activation and is lost in plasma cells. It is also found on granulocytes, monocytes, a small
subset of thymocytes and neural cells. CD24/HSA may play a role in regulation of B cell
proliferation and differentiation. A role for CD24 in promoting homotypic adhesion has been
postulated. It may also serve as a ligand for CD62P/P-selectin, a calcium-dependent lectin.
Clone: 30-F1 Isotype: IgG2c Rat (LOU/Ws1/M)
Cell Lab Liquid LUO A55738 0.1 mg APC
258
Mouse
Anti-Mouse Antigens
CD25
CD25/IL-2R is expressed on activated cells including T cells, B cells and monocytes. IL-2
induces the expression of the subunit on natural killer cells.
Clone: 3C7 Isotype: IgG2b Rat (Lewis)
The 3C7 monoclonal antibody reacts with an epitope of CD25 that is distinct from that recognized
by mAb 7D4. MAb 3C7 blocks binding of IL-2 to CD25.
Clone: 7D4 Isotype: IgM Rat (Lewis)
The 7D4 monoclonal antibody reacts with an epitope of CD25 that is distinct from that recognized
by mAb 3C7. While 7D4 does not block the binding of IL-2 to CD25, when used in combination
with 3C7 it results in higher levels of inhibition of IL-2-driven proliferation than either of these
monoclonal antibodies alone.
259
Mouse
Anti-Mouse Antigens
CD28
CD28 is a homodimeric protein that is expressed on most thymocytes, at low density on nearly all
CD4
+
and CD8
+
peripheral T lymphocytes, and at very low levels on natural killer cells. Expression
of CD28 is upregulated upon T cell activation. CD28 is a co-stimulatory receptor and is a ligand for
CD80/B7-1 and CD86/B7-2 on B cells and other antigen-presenting cells. It plays an important
role in the interaction between T and B cells.
Clone: 37.51 Isotype: IgG Syrian Hamster
This antibody can be used for in vitro co-stimulation of T cells and NK cells.
Clone: PV-1 Isotype: IgG Armenian Hamster
This antibody can be used for in vitro co-stimulation of T cells and NK cells.
CD30
CD30 is a member of the TNF receptor gene superfamily of proteins. It is expressed predominantly
in the thymus and on activated T cells, with expression peaking at 4-5 days on splenocytes
activated by plate-bound anti-CD3. While some studies suggest a role for CD30 in patterns of
cytokine production by T cells, the precise function of CD30 and its ligand in T cell development,
proliferation and activation has not been clearly defined.
Clone: mCD30.1 Isotype: IgG Armenian Hamster
260
Mouse
Anti-Mouse Antigens
CD31 (PECAM-1)
CD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1), is a type I integral
membrane glycoprotein and a member of the Ig-SF of cell surface receptors. It is constitutively
expressed on the surface of endothelial cells, and concentrated at the junction between them. It is
also weakly expressed on lymphocytes, monocytes, platelets and granulocytes. CD31 interacts
homotypically in cell adhesion assays.
Clone: 390 Isotype: IgG2a Rat (Lewis)
MAb 390 inhibits the aggregation of L-cells transfected with a variant form of CD31. This antibody
can be used for function-blocking studies.
CD38
CD38 is a type II transmembrane glycoprotein expressed at high levels on B-lineage cells and at
lower density on germinal center B cells in unimmunized mice. It has also been reported to be
expressed at moderate levels on a proportion of peripheral T cells and a subpopulation of
thymocytes which are TCR
+
CD4
-
CD8
-
. Monoclonal antibodies specific for CD38 can induce B-
and T-cell proliferation, protect B cells from apoptosis, and inhibit B lymphopoiesis.
Clone: NIMR-5 Isotype: IgG2a Rat
261
Mouse
Anti-Mouse Antigens
CD40
CD40 is a type I cell surface glycoprotein expressed primarily on B-lineage cells. CD40 first
becomes detectable on a subset of small pre-B II cells in bone marrow, with the level of expression
increasing thereafter during B-cell maturation.
Clone: 1C10 Isotype: IgG2a Rat
The agonistic 1C10 mAb closely resembles CD154/gp39 in its ability to stimulate proliferation of
small, resting B cells in the absence of other cofactors.
CD44 (Ly-24 / Pgp-1)
CD44 is a type I transmembrane glycoprotein expressed on many cell types including B cells,
monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 has
been implicated in various processes such as hematopoiesis, lymphocyte homing, leukocyte
activation, tumor metastasis, and development.
Clone: KM201 Isotype: IgG1 Rat (Fischer)
The KM201 mAb can inhibit lymphopoiesis and hematopoiesis in long-term bone marrow cultures
and costimulate the activation of splenic CD4
+
T cells.
262
Mouse
Anti-Mouse Antigens
CD45 (LCA)
CD45 is the leukocyte common antigen (LCA) and is expressed on all cells of hematopoietic origin
except erythrocytes. CD45 exists as a number of different isoforms which result from alternative
RNA splicing of exons 4, 5, and 6.
Clone: I3/2.3 Isotype: IgG2b Rat
The I3/2.3 mAb recognizes an epitope common to all CD45 isoforms. CD45 has been implicated
as playing a key role in antigen-driven proliferation of T cells and thymic development.
263
Mouse
Anti-Mouse Antigens
CD45.1 (Ly-5.1)
CD45.1/Ly-5.1 is an allelic variant of the leukocyte common antigen (LCA).
Clone: A20 Isotype: IgG2a Mouse (A.SW)
MAb A20 reacts with leukocytes from mouse strains that express the Ly-5.1 allotype (RIII, SJL/J,
STS/A, DA). It does not react with leukocytes from mouse strains that express the Ly-5.2 allotype.
MAb A20 may be useful for identifying the allotype of leukocytes in transgenic and knockout
mice.
CD45.2 (Ly-5.2)
CD45.2/Ly-5.2 is an allelic variant of the leukocyte common antigen (LCA).
Clone: 104 Isotype: IgG2a Mouse (SJL)
MAb 104 reacts with leukocytes from mouse strains that express the Ly-5.2 allotype (AKR, Balb/c,
CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). It
does not react with leukocytes from mouse strains that express the Ly-5.1 allotype. MAb 104 may
be useful for identifying the allotype of leukocytes in transgenic and knockout mice.
Cell Lab Liquid LUO A55736 0.1 mg PE
264
Mouse
Anti-Mouse Antigens
CD45R (B220)
B220 represents a restricted form of the CD45 glycoprotein family which is expressed on all
hematopoietic cells except mature erythrocytes.
Clone: RA3-6B2 Isotype: IgG2a Rat
The RA3-6B2 MAb primarily recognizes cells of B-lineage from pro-B cells through mature B
lymphocytes. It also reacts with certain activated T cells, as well as non-MHC-restricted lytically
active lymphokine-activated killer cells.
CD45RB
CD45RB represents an exon B-dependent form of CD45 glycoprotein. It is expressed at high
density on peripheral B cells, the suppressor/cytotoxic subpopulation of T cells, a subset of T
helper cells, most thymocytes and at low density on macrophages and dendritic cells.
Clone: C363.16A Isotype: IgG2a Rat
265
Mouse
Anti-Mouse Antigens
CD45RC
CD45RC represents an exon C-dependent isoform of CD45 glycoprotein. It is expressed at high
density on all B lymphocytes, at intermediate density on most CD8
+
splenic T cells, and on two
subpopulations of CD4
+
splenic T cells with high and low density of expression. CD45RC is also
present on a small subset of thymocytes.
Clone: C455.1F Isotype: IgG2a Rat
Clone: GL24 Isotype: IgM Rat
Cell Lab Liquid LUO 736565 0.5 mg FLMA
Cell Lab Liquid LUO 736571 0.1 mg FLMA
CD49b (VLA-2)
Murine CD49b, also known as very late antigen-2 (VLA-2) and 2 integrin, is expressed on the
majority of natural killer (NK) cells and a small subpopulation of T cells in all mouse strains tested.
Most cells that express the NK1.1 antigen also express CD49b. However, small subsets of CD49b
+
NK1.1
-
and CD49b
-
NK1.1
+
can be found, especially among the CD3
+
population of T
lymphocytes.
Clone: DX5 Isotype: IgM Rat (Lewis)
NK cells cultured in the presence of IL-2 progressively lose reactivity with mAb DX5 as a
consequence of cellular proliferation. Therefore, mAb DX5 can be used to define functionally
distinct subsets of murine NK cells.
266
Mouse
Anti-Mouse Antigens
CD49d (VLA-4)
CD49d/VLA-4 is a member of the 1 integrin family of cell adhesion molecules and is a counter-
receptor for both VCAM-1 (CD106) and fibronectin. It is expressed at low density on resting
peripheral lymphocytes and monocytes but is upregulated on all lymphoid and myeloid cells in long-
term bone marrow cultures.CD49d/VLA-4 mediates a variety of cell-cell and cell-matrix interactions
and is of particular importance for lymphopoiesis and extravasation of lymphocytes into sites of
inflammation.
Clone: PS/2 Isotype: IgG2b Rat (Fischer)
This antibody can be used for in vitro inhibition of lymphopoiesis as well as for in vitro retardation
of myelopoiesis.
CD49e (VLA-5)
The 5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor is expressed on
thymocytes, activated T cells, mast cells, and a variety of mouse cell lines.
Clone: 5H10-27 Isotype: IgG2a Rat (Lewis)
Soluble 5H10-27 mAb inhibits VLA-5-mediated functions in vitro. Immobilized 5H10-27 mAb has
also been demonstrated to co-stimulate the proliferative response of CD8
+
T cells to plate-bound
anti-CD3 monoclonal antibody.
267
Mouse
Anti-Mouse Antigens
CD54 (ICAM-1)
CD54, a member of the immunoglobulin superfamily of cell surface receptors and also known as
Intercellular Adhesion Molecule-1 (ICAM-1), is a type I transmembrane glycoprotein that mediates
important cell/cell interactions. CD54 is expressed on endothelial cells, dendritic cells,
keratinocytes and lymphocytes. Its expression is upregulated by inflammatory mediators such as
IFN-, IL-1, TNF-, lipopolysaccharide and phorbol esters. Endothelial CD54 contributes to the
extravasation of leukocytes from blood vessels, particularly in areas of inflammation. CD54 on
antigen-presenting cells contributes to antigen-specific T cell activation, presumably by enhancing
interactions between T cells and antigen-presenting cells. These adhesion reactions are mediated
via the binding of CD54 to its major ligands, LFA-1 and Mac-1.
Clone: YN1/1.7.4 Isotype: IgG2b Rat
This antibody can be used for in vitro blocking studies.
CD62L (L-selectin)
CD62L is a member of the selectin family of cell adhesion molecules. It is expressed on circulating
granulocytes, monocytes and most lymphocytes. Its expression on neutrophils and lymphocytes is
rapidly down-regulated upon activation. The level of CD62L/L-selectin expression can be used to
help distinguish naive T cells from effector/memory T cells.
Clone: MEL-14 Isotype: IgG2a Rat (Fischer)
This antibody can be used for in vitro blocking of adhesion as well as for cytotoxicity studies.
268
Mouse
Anti-Mouse Antigens
CD69 (VEA)
CD69, a disulfide-linked heterodimer, is the Very Early Activation Antigen (VEA). It is not expressed
on resting lymphocytes but is among the first antigens to appear upon in vitro activation of T cells,
B cells and NK cells.
Clone: H1.2F3
Isotype: IgG Armenian Hamster
The H1.2F3 mAb can augment PMA-induced T cell stimulation. This antibody can be used for in
vitro T cell and NK cell activation.
Cell Lab Liquid LUO A88573 0.1 mg PC7
CD71
CD71 is the transferrin receptor, a cell surface molecule essential for the growth of normal and
neoplastic cells. It is expressed at very low density on resting lymphocytes but is greatly
upregulated during responses to antigens and mitogens. The expression of CD71 is a marker for
both proliferation and differentiation, the latter being employed for differentiating hematopoietic
cells. In general, CD71 expression is highest on immature proliferating cells and declines with
maturation and differentiation.
Clone: R17217 Isotype: IgG2a Rat
This antibody can be used for in vitro inhibition of proliferation.
269
Mouse
Anti-Mouse Antigens
CD72.1 (Lyb-2.1)
CD72.1/Lyb-2.1 is a type II integral membrane protein expressed as a homodimer on the surface of
mouse B cells in strains with the Lyb-2.1 allotype. It is the ligand for CD5.
Clone: 10-1.D2 Isotype: IgG2b Mouse
MAb 10-1.D.2 blocks binding of CD5 to the B cell surface, and it enhances in vitro B cell
responses.
CD79b (B29 / Ig)
CD79 is a 80/90 kDa disulfide-linked heterodimer composed of an chain (CD79a/mb-1) and a
chain (CD79/B29) that associates non-covalently with membrane Ig to form the B cell receptor
(BCR) complex. Its expression is restricted to B lymphocytes, first appearing on the surface at the
pro-B cell stage prior to productive Ig gene rearrangement, and remaining through all stages of B
cell differentiation prior to plasma cells. It has been proposed that the CD79b complex on pro-B
cell surfaces may function to induce early B-cell differentiation.
Clone: HM79-12 Isotype: IgG Armenian Hamster
270
Mouse
Anti-Mouse Antigens
CD80 (B7-1)
CD80/B7-1 is a member of the immunoglobulin superfamily and, along with CD86/B7-2,
participates in T cell co-stimulation via interaction with CD28 and CD152/CTLA-4. CD80 is
constitutively expressed on dendritic cells, monocytes and peritoneal macrophages. It can be
upregulated on B cells by various means including activation by LPS, IL-4, and the cross-linking of
surface Ig. Expression of CD80 on B lymphocytes is greatly enhanced after activation with peak
expression occurring between 48 and 72 hours.
Clone: 1G10 Isotype: IgG2a Rat
CD81 (TAPA-1)
CD81/TAPA-1 is a member of the transmembrane 4 integral membrane protein family and is
expressed on many types of cells. Interaction of immature thymocytes with CD81 on a subset of
thymic stromal cells is essential for development of T cells with / T cell receptors.
Clone: 2F7 Isotype: IgG Armenian Hamster
MAb 2F7 blocks thymocyte maturation in fetal organ cultures.
271
Mouse
Anti-Mouse Antigens
CD86 (B7-2)
CD86/B7-2 is a member of the immunoglobulin superfamily and, along with CD80/B7-1,
participates in T cell costimulation via interaction with CD28 and CD152/CTLA-4. CD86 is
expressed at very low density in normal tissues but is rapidly upregulated on stimulated B cells,
monocytes and dendritic cells. Since CD86/B7-2 has rapid kinetics of induction, it is believed to be
the major CD28 ligand expressed early in the immune response.
Clone: 2D10 Isotype: IgG2b Rat
Clone: GL1 Isotype: IgG2a Rat (Louvain)
This antibody can be used for in vitro blocking assays.
CD90 (Thy-1)
CD90/Thy-1 is a GPI-anchored molecule and one of the smallest members of the immunoglobulin
superfamily, consisting of a single V-set domain. In the mouse it is expressed on all thymocytes and
T cells. It is also expressed in the brain and at varying levels in a number of other non-lymphoid
tissues.
Clone: G7 Isotype: IgG2c Rat (Lewis)
MAbs specific for CD90/Thy-1 can be co-mitogenic with phorbol esters. This activation requires
the presence of the T cell receptor. This antibody can be used for in vitro T cell activation as well
as for induction of apoptosis.
272
Mouse
Anti-Mouse Antigens
CD90.2 (Thy-1.2)
CD90.2, a member of the immunoglobulin superfamily of cell surface receptors, represents the Thy
-1.2 alloantigen expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T
cells of most mouse strains. It is also expressed in the brain and at varying levels on other non-
lymphoid tissues.
Clone: 30-H12 Isotype: IgG2b Rat
The 30-H12 mAb does not react with mouse and rat strains bearing the Thy-1.1 alloantigen (e.g.,
AKR/J, PL). This antibody can be used for in vitro T-cell depletion.
CD94
CD94 receptor subunit is a 70kDa type II transmembrane glycoprotein. This antigen is expressed
on the surface of NK cells, NK T cells and some memory phenotype CD8
+
cells. When CD94 is
paired with NKG2A, C, or E subunit, it binds the nonclassical MHC class Ib molecule Qa-1b and
results in a CD94
high
population by flow cytometry. Approximately half of NK cells do not express
NKG2A, C or E; these cells express CD94 on the surface in a form that does not bind Qa-1b and
is believed to be nonfunctional, producing a CD94
low
population by flow cytometry. CD94 is
thought to play a role in the adhesion and activation of the NK cell lineage.
Clone: 18D3 Isotype: IgG2a Rat (Lewis)
MAb 18D3 reacts with the mouse CD94 receptor subunit.
273
Mouse
Anti-Mouse Antigens
CD102 (ICAM-2)
Mouse ICAM-2 (CD102) cell surface glycoprotein is a ligand for the leucocyte integrin
CD11a/CD18 (LFA-1). CD102 is constitutively expressed on T cells, B cells, and at high levels on
vascular endothelial cells. It is also expressed on a variety of leukocyte cell lines.
Clone: 3C4 Isotype: IgG2a Rat (Lewis)
The 3C4 (mIC2/4) mAb blocks the interaction between ICAM-2 and LFA-1.
CD103
CD103 is expressed as a heterodimer on all intestinal intraepithelial lymphocytes (IEL), on dendritic
epidermal cells (DEC), on subpopulations of peripheral CD8
+
T cells in lymph node and lamina
propria, and on distinct subsets of fetal, neonatal, and adult thymocytes. CD103 may play an active
role in the interaction of T lymphocytes with epithelia during T-cell maturation and effector functions.
Clone: 2E7 Isotype: IgG Armenian Hamster
274
Mouse
Anti-Mouse Antigens
CD104 (4 integrin)
CD104 represents the integrin 4 subunit that associates non-covalently with integrin 6 (CD49f)
to form the 64 integrin heterodimer. The 64 complex is expressed on the basal surface of a
variety of epithelial cell types, particularly on the hemidesmosomes of stratified squamous epithelia.
It is also found on peripheral nerves, in certain subsets of endothelial cells, and on immature
thymocytes. Hemidesmosomal 64 plays an important role in the adhesion of epithelia to
basement membranes via interactions with laminin and/or kalinin anchoring filaments.
Clone: 346-11A Isotype: IgG2a Rat (Fischer 344)
CD105 (Endoglin)
CD105/endoglin is a disulfide-linked homodimer of 90 kDa subunits predominantly expressed on
vascular endothelial cells. Although the function of endoglin is unknown, an Arg-Gly-Asp sequence
in the extracellular region suggests a possible role in adhesion to an integrin ligand.
Clone: MJ7/18 Isotype: IgG2a Rat
CD106 (VCAM-1)
CD106, also known as vascular cell adhesion molecule-1 (VCAM-1) and whose ligand is
CD49d/VLA-4, is a member of the immunoglobulin superfamily of cell adhesion molecules. It is
expressed on activated microvascular endothelial cells in response to inflammation. It also plays a
significant role in hemopoiesis via its ability to maintain lymphoid and myeloid precursors on stromal
cell monolayers in Whitlock-Witte long-term BM cultures.
Clone: M/K-2 Isotype: IgG1 Rat (Fischer)
This antibody is useful for the blockage of lymphocyte adhesion. It can be used for inhibition of
lymphopoiesis.
275
Mouse
Anti-Mouse Antigens
CD107a (LAMP-1)
CD107a is a type I integral membrane protein consisting of a core protein of approximately 40 kDa
which is heavily glycosylated to form heterogeneous mature glycoproteins of 100-140 kDa. It is one
of the two major glycoproteins in lysosomal membranes which can be used to distinguish
lysosomes from other organelles. CD107a is expressed primarily on epithelial cells and
macrophages but is also found on T cells, granulocytes and endothelial cells. Cell surface LAMP-1
may participate in intercellular adhesion and adhesion to extracellular matrix.
Clone: 1D4B Isotype: IgG2a Rat (Sprague-Dawley)
CD107b (LAMP-2)
CD107b is a type I integral membrane protein consisting of a core protein of approximately 40 kDa
which is heavily glycosylated to form heterogeneous mature glycoproteins of 100-140 kDa. It is one
of the two major glycoproteins in lysosomal membranes which can be used to distinguish
lysosomes from other organelles. CD107b is similar in structure to Mac-1 and is expressed
primarily on epithelial cells and macrophages in a variety of organs in normal and Beige mutant
mice.
Clone: ABL-93 Isotype: IgG2a Rat (Sprague-Dawley)
276
Mouse
Anti-Mouse Antigens
CD117
CD117, also known as c-kit, belongs to subclass III within the family of transmembrane tyrosine
kinase receptor proteins. It is expressed on most hematopoietic progenitor cells, including colony-
forming cells reactive to IL-3, GM-CSF and M-CSF, but not on B-lineage precursors responsive to
IL-7. CD117 is also expressed on mast cells, melanocytes, spermatogonia and oogonia. Interaction
of CD117 with its counter receptor, c-kit ligand (also known as stem cell growth factor, steel factor
and mast cell growth factor) is critical for the development of hematopoietic, gonadal and pigment
stem cells.
Clone: 2B8 Isotype: IgG2b Rat (Wistar)
MAb 2B8 is useful as a major lineage marker.
CD122 (IL-2R)
CD122 represents the chain of the // heterotrimer that forms the high affinity IL-2 receptor
complex. In mouse spleen, CD122 is expressed on approximately 30% of CD8
+
cells and all NK
cells, but < 1% of B cells and CD4
+
T cells. In the thymus, its expression is confined to CD4
-
CD8
+
single positive and CD4
-
CD8
-
double negative cells. Cytoplasmic domains of the IL-2R chain are
involved in IL-2-mediated signaling and, via the interaction of IL-2 and its receptor complex, may be
involved in the generation and differentiation of T lymphocytes.
Clone: 5H4 Isotype: IgG2a Rat (Lewis)
MAb 5H4 does not block IL-2 binding.
277
Mouse
Anti-Mouse Antigens
CD152 (CTLA-4)
CD152 is expressed on activated T lymphocytes 2-3 days after stimulation via the T cell receptor.
Unlike CD28, its expression is restricted to activated T cells with maximal protein expression 48
hours post-activation. It is structurally similar to CD28 and has the same CD80/B7-1 and
CD86/B7-2 counter-receptors. Although the precise role of CTLA-4 in T-cell activation is not
completely understood, it may function to down-regulate T cell activation.
Clone: 1B8 Isotype: IgG Armenian Hamster
Clone: 9H10 Isotype: IgG Syrian Hamster
Soluble MAb 9H10 can be used for in vitro blocking of CD152/CTLA-4. Cross-linking of CTLA-4
by 9H10 promotes in vitro CTLA-4-mediated signaling.
CD154 (CD40L)
CD154, formerly known as CD40 ligand and gp39, is a type II integral membrane protein and a
member of the tumor necrosis factor (TNF) family of ligands. It is an important accessory molecule
in T cell-B cell costimulatory interactions and is expressed predominantly on activated CD4
+
T
lymphocytes. It is also found on the surface of activated Th0, Th1 and Th2 T cell clones. Its
expression is transient and cyclosporin-sensitive.
Clone: MR1 Isotype: IgG Armenian Hamster
278
Mouse
Anti-Mouse Antigens
Bax
Bax is a 20 - 22 kDa member of the Bcl-2 family of proteins involved in regulation of programmed
cell death, or apoptosis. In murine thymocytes, it is expressed primarily as a cytoplasmic protein.
Over-expression of Bax promotes apoptosis by formation of homodimers and through
heterodimerization with Bcl-2, an inhibitor of apoptosis.
Clone: 5B7 Isotype: IgG1 Mouse
MAb 5B7 recognizes an epitope between amino acids 3-16 of murine Bax. It reacts only with
mouse Bax and can bind both soluble and detergent-altered forms of Bax as well as Bax-Bcl-2
and Bax-Bcl-x
L
heterodimers.
Bcl-2
Bcl-2 is a 26 kDa member of the family of proteins involved in regulation of programmed cell death,
or apoptosis. It is expressed in a variety of both normal and neoplastic tissues and appears to be
membrane-bound since it is detectable in crude membrane and nuclear fractions, but not in the
soluble fraction of cell lysates. Overexpression of Bcl-2 can prevent apoptosis, while formation of
heterodimers with Bax, another Bcl-2-related protein, inhibits Bcl-2s ability to promote cell survival.
Clone: 10C2 Isotype: IgG1 Mouse
MAb 10C2 reacts with an epitope between amino acids 61-76 of mouse Bcl-2. The antibody also
reacts with rat Bcl-2.
BiP
The immunoglobulin heavy chain binding protein, BiP, is a member of the highly conserved HSP70
family of heat shock proteins and is identical to the glucose regulated protein grp78. BiP was
originally described in B cells but is now known to be distributed in a wide variety of tissues. BiP
functions primarily as a molecular chaperone in non-secreting pre-B cells where it remains
associated with m heavy chains to prevent secretion until light chains are synthesized for assembly.
Clone: 76-E6 Isotype: IgG1 Rat
This antibody is useful for studies of Ig assembly and secretion.
279
Mouse
Anti-Mouse Antigens
BP-1 (6C3 / Ly-51)
BP-1/6C3/Ly-51 is a type II disulfide-linked homodimeric transmembrane glycoprotein. It is
expressed at high levels on BM stromal cell lines which support in vitro B lymphopoiesis, and on a
variety of mouse tissues known to possess aminopeptidase activity. Subsets of normal pre-B and B
cells express low levels of BP-1/6C3, which is rapidly upregulated on pre-B cells in the presence
of IL-7.
Clone: FG35.4 Isotype: IgG1 Rat
This antibody is useful for studies of B cell ontogeny.
GM-CSF
Clone: MP1-22E9 Isotype: IgG2a Rat
ELISA: MP1-22E9 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for
quantifying mouse GM-CSF protein levels. Purified MP1-22E9 antibody should be paired with
biotinylated MP1-31G6 antibody (PN 731829) as the detection antibody, with purified
recombinant mouse GM-CSF as the standard. Western Blotting: The purified MP1-22E9 antibody
is also useful for Western blotting applications in conjunction with AP-labeled Goat Anti-Rat Ig, PN
732647.
Clone: MP1-31G6 Isotype: IgG1 Rat
ELISA: MP1-31G6 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for
quantifying mouse GM-CSF protein levels. Biotinylated MP1-31G6 antibody should be paired
with purified MP1-22E9 antibody (PN 731827) as the capture antibody. It is recommended to use
purified recombinant mouse GM-CSF as the standard.
280
Mouse
Anti-Mouse Antigens
H-2D
b
The classical MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene
complex and consist of heterodimers of highly polymorphic chains noncovalently associated with
the invariant 2-microglobulin. These antigens are expressed on most nucleated cells but
expression varies on different cell types. MHC Class I molecules present endogenously synthesized
peptides to CD8
+
T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens
expressed on thymic epithelial cells regulate the positive and negative selection of CD8
+
T cells
during T cell ontogeny.
Clone: 28-14-8 Isotype: IgG2a Mouse (C3H/HeJ)
MAb 28-14-8 binds to the 3 domain of H-2Db in the presence or absence of 2 microglobulin. It
cross reacts with the 3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The
antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of
target cells by cytotoxic T lymphocytes, but it does not block recognition of H-2Ld-positive target
cells by Ly-6G2-positive NK cells.
Clone: 27-11-13S Isotype: IgG2a Mouse (BALB/c)
Mab 27-11-13S recognizes with the 3 domain of H-2D
b
class I MHC antigen. The antibody
cross-reacts with the 3 domain of H-2L
d
, H-2D
q
and H-2L
q
, but not H-2K
d
or H-2D
d
. Reactivity
with haplotypes k, f, p, r, and s has not been observed.
281
Mouse
Anti-Mouse Antigens
H-2D
d
Clone: 34-5-8S Isotype: IgG2a Mouse (C3H/HeJ)
MAb 34-5-8S reacts with a conformational epitope on H-2D
d
MHC Class I found on the N-
terminal domains of 1 and 2 chains when complexed with 2-microglobulin. The antibody does
not react with H-2D
d
chains synthesized in vitro. Weak cross-reactivity with cells from mice of
the H-2
b
, H-2
q
, and H-2
s
haplotypes has been observed by flow cytometric analysis. Reactivity with
cells from mice of the H-2
f
, H-2
k
, H-2
p
, and H-2
r
haplotypes has not been observed. MAb 34-5-8
has been reported to block the recognition of H-2D
d
by Ly-49A
+
, Ly-49C
+
and Ly-49G2
+
natural
killer cells.
H-2D
k
Clone: 15-5-5S Isotype: IgG2a Mouse (C3H.SW)
MAb 15-5-5S reacts with the H-2Dk class I alloantigen. It cross-reacts with H-2K
d
and with cells
from mice with the H-2
f
haplotype. The antibody does not react with other (e.g., a, b, p, q, r, s)
haplotypes.
H-2K
d
/H-2D
d
Clone: 34-1-2S Isotype: IgG2a Mouse (C3H)
The 34-1-2S mAb binds to a common determinant in the 3 domains of H-2K
d
and H-2D
d
in the
presence or absence of 2 microglobulin. It cross reacts with the 3 domain of H-2K
b
and with
the H-2K MHC class I alloantigen of the s, r, q, or p haplotypes.
282
Mouse
Anti-Mouse Antigens
H-2K
k
Clone: 16-3-22S Isotype: IgG2a Mouse
MAb 16-3-22S reacts with the H-2K
k
MHC class I alloantigen. Cross-reactivity with splenocytes
of SJL/Hsd mice has been observed by flow cytometric analysis.The antibody does not react with
other (e.g., b, d, q) haplotypes.
Clone: 36-7-5 Isotype: IgG2a Mouse
Cross-reactivity of mAb 36-7-5 with splenocytes of SJL/Hsd mice has been observed by flow
cytometric analysis. The antibody does not react with other (e.g., b, d, q) haplotypes.
I-A
b
(A
b
)
Clone: 25-9-3 Isotype: IgM Mouse (C3H)
MAb 25-9-3 reacts specifically with the I-A
b
haplotype of MHC Class II molecules. Class II
antigens are predominantly expressed on antigen-presenting cells including B lymphocytes,
macrophages, dendritic cells and certain epithelial cells. Reactivity with other haplotypes has not
been observed.
I-A
d
Clone: 34-5-3 Isotype: IgG2a Mouse
283
Mouse
Anti-Mouse Antigens
I-E
k
Cells from mice of the H-2
b
, H-2
f
, H-2
g
, H-2
q
, and H-2
s
haplotypes do not express I-E antigen.
MAb 17-3-3S reacts with the I-E
k
class II alloantigen on cells from mice of the H-2
r
haplotype;
reactivity with H-2
d
and H-2
p
haplotypes has not been observed.
MAb 14-4-4S reacts with the I-E
k
class II alloantigen on cells from mice of the H-2
d
, H-2
p
, and H
-2
r
haplotypes. MAb 14-4-4S has been reported to cross-react with the rat MHC class II
alloantigen RT1D.
IFN-
Mouse Interferon- (IFN-) is a 16-20 kDa pleiotrophic cytokine, produced by activated T cells and
NK cells. It is involved in the regulation of almost all immune and inflammatory responses. IFN- is
involved in the activation, growth and differentiation of T cells, B cells, macrophages, NK cells as
well as other cell types. INF- enhances MHC expression on antigen-presenting cells including
epithelial, endothelial and connective tissue cell and monocyte cell lines. It also has weak antiviral
activity and acts as a macrophage-activating factor (MAF) in tumor cytotoxicity leading to anti-tumor
effects.
Clone: XMG1.2 Isotype: IgG1 Rat
284
Mouse
Anti-Mouse Antigens
IgA
Clone: 11-44-2 Isotype: IgG1 Rat
MAb 11-4-2 reacts with the heavy chain of mouse IgA. It does not react with IgM, IgD, or IgG.
The antibody can be used as a primary staining reagent for detection of membrane-bound and
intracytoplasmic IgA, and as a capture or detection antibody in ELISA. This antibody has not been
tested for immunohistochemical applications or as an immunoprecipitation reagent.
Cell Lab Liquid LUO 732418 1 mL HRP
Cell Lab Liquid LUO 732417 1 mL AP
IgD
Clone: 11-26c Isotype: IgG2a Rat
MAb 11-26c recognizes the heavy chain of mouse IgD of all mouse strains. It does not react
with IgM, IgG or IgA. The antibody is useful as a primary staining reagent for IgD
+
B lymphocytes
and for immunoprecipitation of murine IgD.
285
Mouse
Anti-Mouse Antigens
IgE
Clone: 23G3 Isotype: IgG1 Rat
MAb 23G3 reacts with the heavy chain of mouse IgE of all mouse strains. It does not react with
IgM, IgD, IgG or IgA. The antibody can be used as a primary staining reagent for detection of
membrane-bound and intracytoplasmic IgE, and as a capture or detection antibody in ELISA. This
antibody has not been tested for immunohistochemical applications or as an immunoprecipitation
reagent.
IgG2a
Clone: SB84a Isotype: IgG1 Rat
Cell Lab Liquid LUO A55763 0.5 mg UNLB
Cell Lab Liquid LUO A55730 0.5 mg FITC
IgG2b
Clone: LO-MG2b-2 Isotype: IgG1 Rat
286
Mouse
Anti-Mouse Antigens
IgG3
Clone: LO-MG3-7 Isotype: IgM Rat
IgM
Clone: 1B4B1 Isotype: IgG1 Rat
MAb 1B4B1 reacts with the heavy chain of mouse IgM of all mouse strains. It does not react
with IgD, IgG or IgA. The antibody can be used as a primary staining reagent for detection of
membrane-bound and intracytoplasmic IgM, as a 2
nd
step reagent for mouse IgM MAbs and as a
capture or detection antibody in ELISA. This antibody has not been tested for
immunohistochemical applications or as an immunoprecipitation reagent.
287
Mouse
Anti-Mouse Antigens
IL-2
Clone: JES6-1A12 Isotype: IgG2a Rat
ELISA: JES6-1A12 monoclonal antibody is useful as the capture antibody in a sandwich ELISA for
quantifying mouse IL-2 protein levels. Unlabeled JES6-1A12 antibody (PN IM3020, or PN
736589) should be paired with biotinylated JES6-5H4 monoclonal antibody (PN IM3019, or PN
731556) as the detection antibody. Or, conversely, biotinylated JES6-1A12 antibody (PN 731790)
may be paired with unlabeled JES6-5H4 antibody (PN 731791) as the capture antibody. Western
Blotting: the unlabeled JES6-1A12 antibody is also useful for immunoprecipitation and Western
Clone: JES6-5H4 Isotype: IgG2b Rat
ELISA: JES6-5H4 monoclonal antibody is useful as the detection antibody in a sandwich ELISA for
quantifying mouse IL-2 protein levels. Biotinylated JES6-5H4 antibody (PN IM3019, or PN
736556) should be paired with unlabeled / purified JES6-1A12 monoclonal antibody (PN IM3020,
or PN 731789) as the capture antibody. Or, conversely, unlabeled JES6-5H4 antibody (PN
731791) may be paired with biotinylated JES6-1A12 antibody (PN 731790) as the detection
antibody.
IL-3
Clone: MP2-8F8 Isotype: IgG1 Rat
ELISA: MP2-8F8 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for
quantifying mouse IL-3 protein levels. Unlabeled MP2-8F8 antibody (PN 731813) should be
paired with biotinylated MP2-43D11 monoclonal antibody (PN 731815) as the detection antibody.
Immunofluorescence / Flow Cytometry: MP2-8F8 antibody is useful for intracytoplasmic staining
and flow cytometric analysis to identify and enumerate IL-3-positive cells within mixed cell
populations.
Clone: MP2-43D11 Isotype: IgG2a Rat
ELISA: MP2-43D11 monoclonal antibody is useful as a detection antibody in a sandwich ELISA
for quantifying mouse IL-3 protein levels. Biotinylated MP2-43D11 antibody (PN 731815) should
be paired with unlabeled MP2-8F8 monoclonal antibody (PN 731813) as the capture antibody.
Western Blotting: the unlabeled MP2-43D11 antibody is also useful for Western blotting
applications.
288
Mouse
Anti-Mouse Antigens
IL-4
Clone: BVD4-1D11 Isotype: IgG2b Rat
ELISA: BVD4-1D11 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for
quantifying mouse IL-4 protein levels. Unlabeled BVD4-1D11 antibody (PN 731792) should be
paired with biotinylated BVD6-24G2 monoclonal antibody (PN 731794) as the detection antibody.
Immunofluorescence / Flow Cytometry: BVD4-1D11 antibody is useful for intracytoplasmic
staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell
populations. Western Blotting: the unlabeled BVD4-1D11 antibody is also useful for Western
Clone: BVD6-24G2 Isotype: IgG1 Rat
ELISA: BVD6-24G2 monoclonal antibody is useful as a detection antibody in a sandwich ELISA
for quantifying mouse IL-4 protein levels. Biotinylated BVD6-24G2 antibody (PN 731794) should
be paired with unlabeled BVD4-1D11 monoclonal antibody (PN 731792) as the capture antibody.
Immunofluorescence / Flow Cytometry: BVD6-24G2 conjugated forms (PN IM3022, and PN
IM3023) are useful for intracytoplasmic staining and flow cytometric analysis to identify and
enumerate IL-4-positive cells within mixed cell populations.
IL-5
Clone: TRFK4 Isotype: IgG2a Rat
ELISA: TRFK4 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for
quantifying mouse IL-5 protein levels. Biotinylated TRFK4 antibody (PN 731797) should be paired
with unlabeled TRFK5 monoclonal antibody (PN 731795) as the capture antibody.
Clone: TRFK5 Isotype: IgG1 Rat
ELISA: TRFK5 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for
quantifying mouse IL-5 protein levels. Unlabeled TRFK5 antibody (PN 731795) should be paired
with biotinylated TRFK4 monoclonal antibody (PN 731797) as the detection antibody.
Immunofluorescence / Flow Cytometry: TRFK5 antibody is useful for intracytoplasmic staining and
flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.
Western Blotting: the unlabeled TRFK5 antibody is also useful for Western blotting applications.
289
Mouse
Anti-Mouse Antigens
IL-6
Clone: MP5-32C11 Isotype: IgG2a Rat
ELISA: MP5-32C11 monoclonal antibody is useful as a detection antibody in a sandwich ELISA
for quantifying mouse IL-6 protein levels. Biotinylated MP5-32C11 antibody (PN 731800)
antibody should be paired with unlabeled MP5-20F3 monoclonal antibody (PN 731798) as the
capture antibody. Western Blotting: the unlabeled MP5-32C11 antibody is useful for Western
Clone: MP5-20F3 Isotype: IgG1 Rat
ELISA: MP5-20F3 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for
quantifying mouse IL-6 protein levels. Unlabeled MP5-20F3 antibody (PN 731798) antibody
should be paired with biotinylated MP5-32C11 monoclonal antibody (PN 731800) as the
detection antibody. Immunofluorescence / Flow Cytometry: MP5-20F3 antibody is useful for
intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-6-positive cells
within mixed cell populations. Western Blotting: the unlabeled MP5-20F3 antibody is also useful
for Western blotting applications.
IL-10
Clone: JES5-2A5 Isotype: IgG1 Rat
ELISA: JES5-2A5 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for
quantifying mouse IL-10 protein levels. Unlabeled JES5-2A5 antibody (PN 731801) should be
paired with biotinylated JES5-16E3 monoclonal antibody (PN 731812) as the detection antibody.
Western Blotting: the unlabeled JES5-2A5 antibody is also useful for Western blotting
applications.
Clone: JES5-16E3 Isotype: IgG2b Rat
ELISA: JES5-16E3 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for
quantifying mouse IL-10 protein levels. Biotinylated JES5-16E3 antibody (PN 731812) should be
paired with unlabeled JES5-2A5 monoclonal antibody (PN 731801) as the capture antibody.
Immunofluorescence / Flow Cytometry: JES5-16E3 forms are useful for intracytoplasmic staining
and flow cytometric analysis to identify and enumerate IL-10-positive cells within mixed cell
populations.
290
Mouse
Anti-Mouse Antigens
IL-17
Clone: TC11-8H4 Isotype: IgG1 Rat
ELISA: TC11-8H4 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for
quantifying mouse IL-17 protein levels. Biotinylated TC11-8H4 antibody (PN 731817) should be
paired with unlabeled TC11-18H10 monoclonal antibody (PN 731818) as the capture antibody.
Clone: TC11-18H10 Isotype: IgG1 Rat
ELISA: TC11-18H10 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for
quantifying mouse IL-17 protein levels. Unlabeled TC11-18H10 antibody (PN 731818) should be
paired with biotinylated TC11-8H4 monoclonal antibody (PN 731817) as the detection antibody.
Immunofluorescence / Flow Cytometry: TC11-18H10 antibody is useful for intracytoplasmic
staining and flow cytometric analysis to identify and enumerate IL-17-positive cells within mixed
cell populations. Western Blotting: the unlabeled TC11-18H10 antibody is also useful for Western
KLRG1
Mouse killer cell lectin-like receptor G1 (KLRG1; formerly known as mouse MAFA or 2F1-Ag) is a
homodimeric member of the lectin-like type 2 transmembrane receptor family that contains
characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains.
These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. Cell surface
expression of KLRG1 is up-regulated by expression of MHC class I molecules. The effect of MHC
class I expression is indirect, and can be mediated by interactions with class I-specific Ly49
inhibitory receptors.
Clone: 2F1 Isotype: IgG Syrian Hamster
The 2F1 mAb stains 30-60% of NK1.1
+
CD3
-
splenocytes, and a small fraction of T cells in all
mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL).
291
Mouse
Anti-Mouse Antigens
Chain
Clone: 187.1 Isotype: IgG1 Rat
MAb 187.1 reacts with light chains. It does not react with immunoglobulins bearing light
chains. The antibody is useful as a primary staining reagent for detection of membrane-bound and
intracytoplasmic immunoglobulin with type light chains. It is also an excellent 2nd step reagent
for use in flow cytometry and ELISA.
Clone: H139-52.1 Isotype: IgG1 Rat
MAb H139-52.1 reacts with light chains. It does not react with immunoglobulins bearing light
intracytoplasmic immunoglobulin with type light chains. It is also an excellent 2
nd
step reagent for
use in flow cytometry and ELISA.
292
Mouse
Anti-Mouse Antigens
Chain
Clone: JC5-1 Isotype: IgG2b Rat
MAb JC5-1 reacts with light chains. It does not react with immunoglobulins bearing light
intracytoplasmic immunoglobulin with type light chains. It is also an excellent 2nd step reagent
for use in flow cytometry and ELISA.
LPAM-1
The lymphocyte Peyers patch adhesion molecule (LPAM-1), also known as integrin 47,
functions as a homing receptor for lymphocyte trafficking and cell-cell interactions. It is expressed
primarily on mucosal lymphocytes, but is also present on NK cells and eosinophils. The 47
heterodimeric complex mediates the binding of lymphocytes to its ligand, mucosal vascular
addressin (MAdCAM-1) on the HEV, thereby directing the homing of lymphocytes into Peyerss
patches and intestinal lamina propria.
Clone: DATK32 Isotype: IgG2a Rat (Fischer)
293
Mouse
Anti-Mouse Antigens
Ly-6A/E (Sca-1)
Ly-6A/E is a member of the Ly-6 family of phosphatidylinositol-anchored proteins. Ly-6A/E exhibits
a pattern of high and low density of expression which is based on differences in the Ly-6.1 and Ly
-6.2 allotypes. Ly-6.1 strains have few Ly-6A/E
+
resting peripheral lymphocytes while Ly-6.2 strains
have relatively high numbers of Ly-6A/E
+
lymphocytes. The expression of Ly-6A/E is dramatically
upregulated upon cellular activation.
Clone: D7 Isotype: IgG2a Rat (Lewis)
Ly-6C
The Ly-6C surface antigen is a member of the Ly-6 family of phosphatidylinositol-anchored
proteins. It is expressed on bone marrow cells, monocytes, macrophages, neutrophils, endothelial
cells and T-cell subsets. Mice with the Ly-6.2 allotype (AKR, C57BL, DBA/2, PL, SJL, SWR, 129)
have subsets of CD4
+
Ly-6C
+
and CD8
+
Ly-6C
+
cells, while Ly-6.1 strains (BALB/c, CBA, C3H/He,
DBA/1, NZB) have only CD8
+
Ly-6C
+
cells. Ly-6C may play a role in the development and
maturation of lymphocytes.
Clone: HK1.4 Isotype: IgG2c Rat
294
Mouse
Anti-Mouse Antigens
Ly-6G (GR-1)
Ly-6G, formerly known as the myeloid differentiation antigen Gr-1, is a GPI-anchored protein. In the
bone marrow, its level of expression is directly correlated with granulocyte differentiation and
maturation. Ly-6G is also expressed transiently on monocytes. The antigen is expressed on
neutrophils in the periphery.
Clone: RB6-8C5 Isotype: IgG2b Rat
Ly49C/F/H/I
Mouse Ly49 receptors exhibit allelic specificity for MHC class I molecules and are thought to serve
to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected
or transformed cells in which class I molecules have been down-regulated. These inhibitory
receptors are also expressed on a subpopulation of mouse CD8
+
T cells.
Clone: 14B11 Isotype: IgG Syrian Hamster
MAb 14B11 reacts with murine Ly49C, Ly49F, Ly49H and Ly49I, members of the lectin-like
homodimeric Ly49 family of cell surface receptors. The 14B11 antibody stains ~ 90% of NK1.1
+
CD3
-
splenocytes, and ~ 6% of CD8
+
spleen cells from C57BL/6 mice. The antibody also stains
30-80% of NK1.1
+
CD3
-
splenocytes in other mouse strains tested (BALB/c, C3H, 129, SJL,
AKR/J).
295
Mouse
Anti-Mouse Antigens
LY49F
The Ly49F antigen is a member of the lectin-like homodimeric Ly49 family of cell surface receptors.
receptors are also expressed on subpopulations of mouse CD8
+
T cells.
Clone: HBF-719 Isotype: IgG1 Mouse (BALB/c)
MAb HBF-719 reacts with the B6 allele of Ly49F. It stains ~ 11% of NK1.1
+
CD3
-
splenocytes,
and 3.5% (2.5 month old) to 10.0% (11 month old) of CD8
+
spleen cells from C57BL/6 mice.
LY49I
receptors are also expressed on subpopulations of mouse CD8
+
T cells.
Clone: YLI-90 Isotype: IgG1 Mouse (BALB/c)
MAb YLI-90 reacts with the B6 allele of Ly49I, a member of the lectin-like homodimeric Ly49 family
of cell surface receptors. The YLI-90 antibody stains ~ 45% of NK1.1
+
CD3
-
splenocytes from
C57BL/6 mice.
296
Mouse
Anti-Mouse Antigens
MAdCAM-1
Mucosal vascular addressin (MAdCAM-1) is a type I transmembrane glycoprotein expressed at high
levels on HEV of Peyers patches and mesenteric lymph nodes, and on flat-walled venules within
the gut lamina propria. It binds integrin 47 through its Ig-SF domain and is also a facultative
ligand for E-selectin. MAdCAM-1 is involved in the recirculation of naive lymphocytes in Peyers
patches and MLNs, the homing of a subpopulation of activated memory T cells to the gut lamina
propria, and the tethering and rolling of lymphocytes on endothelial surfaces prior to extravasation.
Clone: MECA-367 Isotype: IgG2a Rat (Wistar)
MHC Class II (RT1B)
MHC Class II molecules are histocompatibility antigens encoded by the I-A gene complex. Class II
antigens are predominantly expressed on antigen-presenting cells, including B lymphocytes,
macrophages, dendritic cells, and certain epithelial cells. MHC Class II molecules present
exogenously derived antigen to CD4
+
T cells. Class II molecules expressed on thymic stromal cells
play a key role in positive and negative selection of CD4
+
cells during thymopoiesis.
Clone: NIMR-4 Isotype: IgG2b Rat
NCAM
Neural Cell Adhesion Molecule (NCAM) is part of a family of cell surface glycoproteins that plays a
role in embryogenesis and contact-mediated interactions between neural cells and the cellular
matrix. Differential splicing of the RNA transcript from a single gene produces three isoforms of
180 kDa, 140 kDa, and 120 kDa. The highly polysialylated forms of NCAM present in embryos and
neonates are lost during maturation and replaced by less polysialic acid-laden mature forms.
Clone: H28 Isotype: IgG2a Rat
H28.123 reacts with all three mouse NCAM isoforms.
297
Mouse
Anti-Mouse Antigens
NK1.1 (Ly-55)
NK1.1, a member of the NKR-P1 family of cell surface receptors, is a type II integral membrane
glycoprotein with a C-type lectin domain. It is expressed as a disulfide-linked homodimer on all NK
cells, as well as subsets of thymocytes and peripheral T lymphocytes in selected strains of mice (e.
g., C57BL, NZB). NK1.1 mediates cellular activation and differentiation, and is thought to have a
particular role in generating Th2 cells. This product does not react with NK cells of BALB/c mice.
Clone: PK136 Isotype: IgG2b Mouse (C3H x BALB)
NKG2-A/C/E
The CD94/NKG2 heterodimer is a member of the c-type lectin family of inhibitory receptors. Mouse
CD94/NKG2 is a natural killer (NK) cell receptor for the non-classical MHC class I molecule Qa
-1b. NKG2 is expressed on NK cells, lymphokine-activated killer (LAK) T cells, and some CD8
+
memory T cells. With the exception of DBA/2J mice, NKG2 is expressed on NK1.1
+
cells from all
mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL).
Clone: 20d5 Isotype: IgG2a Rat (Lewis)
MAb 20d5 reacts with an epitope common to the NKG2A, NKG2C and NKG2E isoforms of the
CD94/NKG2 heterodimer.
Two-color immunofluorescent staining with the 20d5 antibody reveals two distinct NKG2
+
phenotypes within the NK1.1
+
CD3
-
spleen cell population: NKG2
hi
(50%) and NKG2
low
(50%).
298
Mouse
Anti-Mouse Antigens
Qa-2
Qa-2 is a 40 kDa protein that is encoded by four very similar MHC class Ib genes: Q6, Q7, Q8
and/or Q9 of the H-2 complex. Qa-2 is expressed at higher levels on mature T lyphocytes than on
B cells.
Clone: 695H1-9-9 Isotype: IgG2a Mouse (C3H.KBR)
TCR /
The TCR/ heterodimer portion of the CD3/T cell receptor complex is clonotypic and consists of
Ig-like variable and constant domains. It is expressed during thymopoiesis and on mature peripheral
T lymphocytes of all mouse strains. The TCR/ heterodimer recognizes peptide antigen bound to
MHC antigens and subsequent signal transduction mediated by the invariant chains leads to T cell
activation.
Clone: H57-597 Isotype: IgG Armenian Hamster
TCR /
The TCR / heterodimer of the CD3/T-cell receptor complex is clonotypic and consists of Ig-like
variable and constant domains. It is expressed during thymopoiesis and on the small subpopulation
of / TCR-expressing T cells in the periphery. The majority of T cells present in some epithelial
tissues are /
+
and have limited receptor diversity. The TCR / heterodimer recognizes peptide
antigen bound to MHC antigens, and subsequent signal transduction mediated by the invariant
chains leads to T-cell activation.
Clone: UC7-13D5 Isotype: IgG Armenian Hamster
299
Mouse
Anti-Mouse Antigens
TNF
Clone: MP6-XT3 Isotype: IgG1 Rat
ELISA: MP6-XT3 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for
quantifying mouse TNF protein levels. Western Blotting: The purified MP6-XT3 antibody is also
useful for Western blotting applications in conjunction with AP-labeled Goat Anti-Rat Ig.
Clone: MP6-XT22 Isotype: IgG1 Rat
ELISA: MP6-XT22 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for
quantifying mouse TNF protein levels. Purified MP6-XT22 antibody should be paired with a
suitably labeled detection antibody as the detection antibody. It is recommended to use purified
recombinant mouse TNF as the standard. Western Blotting: The purified MP6-XT22 antibody is
also useful for Western blotting applications in conjunction with AP-labeled Goat Anti-Rat-Ig.
300
Rat
Anti-Rat Antigens
CD45
Clone: OX-1 Isotype: IgG1 Mouse
- Liquid RUO A36700 50 tests PE
CD45RC
CD45RC is the high molecular weight isoform of Leucocyte Common Antigen (also called CD45)
which is a major membrane glycoprotein existing in different forms on different lymphoid cell types.
This antigen is present on B cells (CD45RC
bright
), CD8
+
suppressor/cytotoxic lymphocytes
(CD45RC
dim
), NK cells (CD45RC
dim
), but also a subset of CD4
+
lymphocytes (CD45RC
dim
). It is
also expressed by pre-B cells and weakly by thymocytes. CD45RC expression level depends on
the maturation and activation state of cells allowing the discrimination between resting and
activated cells during maturation process.
Clone: OX-22.2 Isotype: IgG1 Mouse
IgG1
Clone: G1 7E7 Isotype: IgG1 Mouse
Clone: G1 1C5 Isotype: IgG1 Mouse
301
Rat
Anti-Rat Antigens
IgG2a
Clone: 2A 8F4 Isotype: IgG1 Mouse
IgG2b
Clone: 2B 10A8 Isotype: IgG2b Mouse
Cell Lab Liquid LUO 734845 0.5 mg BIMA
IgG2c
Clone: 2C 8F1 Isotype: IgG2a Mouse
IgM
Clone: M 2A1 Isotype: IgG1 Mouse
302
Rat
Anti-Rat Antigens
Chain
Clone: K 4F5 Isotype: IgG1 Mouse
303
Bovine
Anti-Bovine Antigens
Collagen Type II
Source: pooled antisera from goats hyperimmunized with bovine type II collagen. Specificity: reacts
with conformational determinants on bovine type II collagen as demonstrated by ELISA; may react
with type II collagen from other species. Cross Adsorption: collagen types I, III, IV, V and VI
covalently linked to agarose. Purification: affinity chromatography on bovine type II collagen
covalently linked to agarose.
Exhibits < 10% cross reactivity with collagen type I, III, IV, V and VI. The antibody has not been
tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).
304
Chicken
Anti-Chicken Antigens
CD3
CD3 is a member of the T cell receptor-associated CD3 complex. CD3 is expressed on
approximately 30% of thymocytes, 70% of blood mononuclear cells and 60% of spleen cells, but <
1% of cells in the bursa and bone marrow.
Clone: CT-3 Isotype: IgG1 Mouse (BALB/c)
MAb CT-3 recognizes a complex of at least three polypeptides of Mr 20, 19 and 17 kDa on
chicken T cells. It also coprecipitates a polypeptide of 90 kDa from digitonin solubilized T cell
lysates, which can be reduced to two polypeptides of Mr 50 and 40 kDa. It can be used for in vitro
depletion of CD3
+
cells and for in vitro activation of T cells.
CD4
CD4 is a type I transmembrane glycoprotein expressed on approximately 70% of thymocytes, 10%
of spleen cells and 45% of peripheral blood lymphocytes, but < 1% of bursal cells.
MAb CT-4 inhibits both PHA- and Con A-induced proliferative responses of splenocytes to
pokeweed mitogen-induced IL-2 production.
Clone: EP96 Isotype: IgM Mouse (BALB/c)
MAb EP96 inhibits both PHA- and Con A-induced proliferative responses of splenocytes and
pokeweed mitogen-induced IL-2 production.
305
Chicken
CD5
Chicken CD5 is a monomeric type I transmembrane glycoprotein whose extracellular region
consists of three scavenger receptor cysteine-rich domains. During ontogeny, avian CD5 is first
detectable in the thymus and bursa on embryonic day 10, about 2 to 3 days before the first TCR
and surface Ig expression. By the time functional TCR and Ig gene rearrangements and their
expression have been completed, all T and B cells in the primary lymphoid organs express CD5. In
the periphery, / T cells express a relatively high level of CD5, whereas a much lower level of the
CD5 antigen is detected on / T cells and B cells. The control of CD5 expression is activation-
dependent, since activated T cells have up-regulated CD5 expression. As in mammals, CD5 may
play an important role in the control of lymphocyte differentiation and activation.
Clone: 2-191 Isotype: IgG1 Mouse (BALB/c)
CD8
In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two chains, and (ii)
a heterodimer of an chain and a chain. Chicken CD8 is expressed on approximately 80% of
thymocytes, 15% of blood mononuclear cells and 50% of spleen cells, but < 1% of cells in the
bursa and bone marrow. While the vast majority of CD8
+
cells in the thymus, spleen, and blood of
adult chickens express both CD8 - and CD8 -chains, a relatively large proportion of the CD8
+
TCR/ cells in the spleens of embryos and young chicks express only the chain of CD8. Among
intestinal epithelial lymphocytes there is a population of TCR/ CD8 cells in the chicken that is
not found in rodents.
Clone: 3-298 Isotype: IgG2b Mouse (BALB/c)
MAb 3-298 recognizes the CD8 chain of both the chicken and turkey. This antibody can be
used for purification of CD8
+
T cells by positive cell sorting.
306
Chicken
Clone: EP72 Isotype: IgG2b Mouse (BALB/c)
CD8
Chicken CD8 exists in two forms: (i) a homodimer of two chains, and (ii) a heterodimer of an
chain and a chain. While the vast majority of CD8
+
cells in the thymus, spleen and blood of adult
chickens express both CD8 - and CD8 -chains, a relatively large proportion of CD8
+
TCR
+
cells in the spleens of embryos and young chicks express only the chain. CD8 is expressed on
approximately 80% of thymocytes, 45% of PBMC and 50% of spleen cells, but < 1% of bursal
cells.
Clone: EP42 Isotype: IgG2a Mouse (BALB/c)
CD28
Chicken CD28 is a type I transmembrane glycoprotein expressed as a disulfide-linked homodimer.
It is found on most thymocytes, and both V1- and V2-family expressing peripheral /
+
T cells.
Peripheral
+
T cells are negative.
Clone: AV7 Isotype: IgG1 Mouse (BALB/c)
MAbs specific for CD28 antigen are costimulatory on T cells activated by phorbol myristate
acetate (PMA), concanavalin A and mAbs against the TCR. V1- and V2-expressing cells
respond equally well to stimulation with CD28 mAb in combination with PMA. These responses are
resistant to cyclosporin, but are inhibited by herbimycin A, suggesting that CD28 employs a
signaling pathway distinct from that triggered by TCR/CD3.
307
Chicken
CD44
Chicken CD44 is a type I transmembrane glycoprotein expressed on many cell types, including B
cells, most T cells, monocytes and some epithelial cells. CD44 primarily functions as an adhesion
molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing,
leukocyte activation, tumor metastasis and development.
MAb AV6 also reacts with IgM
+
turkey B cells and a subset of turkey T lymphocytes.
CD45
Chicken CD45 is a 190-215 kDa transmembrane glycoprotein that is expressed on all leukocytes.
Clone: LT40 Isotype: IgM Mouse (BALB/c)
Levels of CD45 expression detected by mAb LT40 in the avian thymus are heterogeneous, with
90% of thymocytes expressing fourfold higher levels of surface CD45 (CD45
hi
) than the remaining
10% (CD45
lo
) of thymocytes. The shift from high to low levels of CD45 expression is concomitant
with the transition from CD4
-
CD8
lo
to CD4
+
CD8
+
and before the expression of CD3. MAb LT40,
therefore, is a suitable marker for the transition of CD3
-
CD4
-
CD8
lo
precursors to CD3
-
CD4
+
CD8
+
thymocytes.
308
Chicken
2-microglobulin
Like their mammalian counterparts, avian MHC Class I molecules (also known as B-F antigens)
consist of a highly polymorphic -chain (Mr ~ 44 kDa) non-covalently bound to the invariant 2-
microglobulin subunit (Mr ~ 14 kDa). MHC Class I molecules are expressed on most nucleated
cells where they present endogenously synthesized antigenic peptides to CD8
+
T lymphocytes,
which are usually cytotoxic T cells.
Clone: F21-21 Isotype: IgG1 Mouse (BALB/c)
MAb F21-21 also reacts with turkey 2-microglobulin. This antibody can be used for
immunoaffinity chromatography.
Bu-1
Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated,
disulfide-linked homodimer. Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27%
of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of
macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.
MAb AV20 recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of
both RPL 6 (Bu-1a) and 7 (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in
partially inbred birds. MAb AV20 does not cross react with turkey cells when analyzed by
immunofluorescent staining and flow cytometry.
309
Chicken
Bu-1a
Chicken Bu-1a, a product of the Bu-1a allele, is expressed on the cell surface as a disulfide-linked
homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1a is found on 85-90% of bursal cells,
2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also
expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or
thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit
allelic exclusion.
Clone: 21-1A4 Isotype: IgG1 Mouse (BALB/c)
MAb 21-1A4 does not react with cells from CHA and H.B15 strains by immunofluorescence.
Bu-1b
Chicken Bu-1b, a product of the Bu-1b allele, is expressed on the cell surface as a disulfide-linked
homodimer. In 10-week-old H.B15 and HB2 chickens, Bu-1b is found on 85-90% of bursal cells, 2
-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also
expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or
thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1b does not exhibit
allelic exclusion.
Clone: 5-11G2 Isotype: IgG1 Mouse (BALB/c)
MAb 5-11G2 reacts with lymphoid cells from CHA and H.B14Ab strains, but does not react with
cells from H.B14Aa chickens.
C-kit
Clone: 2C75 Isotype: IgG2a Mouse (BALB/c)
Cell Lab Liquid LUO A55761 0.5 mg BIOT
310
Chicken
ChT1
ChT1, a member of the Ig superfamily with one V-like and one C2-like domain, is a T cell antigen
that is expressed on the surface of embryonic thymocytes (day 10). In young chickens, about 90%
of the thymocytes as well as a subpopulation of peripheral lymphocytes (which represent recent
thymic emigrants) are ChT1 positive. Expression in the periphery declines with age and, in
correlation with partial thymectomy, indicates that ChT1 can be used as an accurate marker for
studying thymic function.
Clone: CT1 Isotype: IgG1 Mouse (BALB/c)
CT1 can block T cell differentiation in vitro, in thymic organ cultures and in thymocyte precursors
cultured on stromal cell monolayers. CT1 also recognizes quail cortical thymocytes.
Ia
The chicken Ia molecule is similar in structure to the murine Ia antigens and human DR antigens,
and consists of an chain (Mr 32 kDa) non-covalently linked to a chain (Mr 27 kDa). It is
expressed on B cells, a subpopulation of the monocyte / macrophage lineage of cells and mitogen-
activated T cells.
Clone: CIa Isotype: IgM Mouse (BALB/c)
This antibody can be used for in vitro inhibition of MLR.
IgA
Clone: A-1 Isotype: IgG2b Mouse (BALB/c)
311
Chicken
IgG
heavy chain of chicken IgG.
Clone: G-1 Isotype: IgG1 Mouse (BALB/c)
IgM
Clone: M-4 Isotype: IgM Mouse (BALB/c)
Clone M-4 is the reagent of choice for in ovo suppression and in vitro activation of chicken
peripheral blood lymphocytes.
Clone: M-1 Isotype: IgG2b Mouse (BALB/c)
Clone M-1is the reagent used for most immunofluorescent staining applications, including flow
cytometry.
Chain
Clone: L-1 Isotype: IgG1 Mouse (BALB/c)
This antibody reacts with -like light chains of chicken immunoglobulin.
312
Chicken
MCAM
Chicken MCAM, a member of the immunoglobulin superfamily of cell surface receptors, consists of
the V-V-C2-C2-C2 Ig domains. It is a single chain glycoprotein with three mRNA splice variants.
One has a short cytoplasmic tail, another has a long tail, and the third seems to lack transmembrane
and cytoplasmic regions. It is highly homologous to gicerin, a molecule involved in neurite
outgrowth and Wilm's kidney tumor progression in the chicken, and to the human melanoma
progression molecule MUC18. Chicken MCAM is expressed by c-kit
+
hematopoietic progenitor
cells in embryonic bone marrow, thymocytes, and capillary endothelial cells. MCAM-expressing
cells sorted from bone marrow are enriched in progenitors capable of differentiating into T cells
when transferred into the appropriate thymic microenvironment. MCAM has been proposed to be
involved in cellular adhesion and homing processes.
Clone: C264 Isotype: IgG2b Mouse (BALB/c)
MHC Class II
MHC Class I
Like their mammalian counterparts, avian MHC Class I molecules (also known as B-F antigens)
consist of a highly polymorphic chain (Mr ~ 44 kDa) non-covalently bound to the invariant 2-
microglobulin subunit (Mr ~ 12 kDa). MHC Class I molecules are expressed on most nucleated
cells where they present endogenously synthesized antigenic peptides to CD8
+
T lymphocytes,
which are usually cytotoxic T cells.
Clone: F21-2 Isotype: IgG1 Mouse (BALB/c)
MAb F21-2 also reacts with turkey MHC Class I. This antibody can be used for immunoaffinity
chromatography.
313
Chicken
Monocytes/Macrophages
Clone: KUL01 Isotype: IgG1 Mouse
The monoclonal antibody KUL01 is useful in the study of the development, distribution, function
and ontogeny of the mononuclear phagocyte system (MPS) of the chicken by exclusively
recognizing the cells of the MPS. It identifies chicken monocytes and macrophages as well as
interdigitating cells and activated microglia cells. This antibody does not react with B (Bu-1
+
)
or T (CD3
+
) lymphocytes.
TCR /
Unlike mouse and human,
+
T cells in the chicken comprise a relatively large subset of T
lymphocytes. The frequency of TCR/ cells is 20-25% of total blood T cells, and may reach
approximately 50% in older chickens. The majority of TCR/ cells in the thymus and blood are
CD4
-
CD8
-
. However, when these cells migrate to the spleen and intestine, most of them begin to
express CD8. TCR/ cells are capable of cytotoxic activity in vitro and may also be involved in
down-regulation of the immune response. They cannot, however, induce a GVH reaction.
Clone: TCR-1 Isotype: IgG1 Mouse (BALB/c)
This antibody can be used for in ovo depletion of T cells. It is a blocking antibody for
hyaluronate binding.
TCR V1
Two distinct subpopulations of TCR/ cells arise sequentially in the chicken thymus during
ontogeny. These two populations (originally known as TCR2 and TCR3) are now known to
represent two distinct V families, V1 and V2, respectively. TCR V1 cells in the thymus express
both CD4 and CD8, while those in the periphery express either CD4 or CD8.
The TCR-2 mAb reacts with approximately 40% of thymocytes, 40-50% of PBMC and 40% of
splenocytes. It can be used for in ovo suppression of V1 T cells.
314
Chicken
TCR V2
Two distinct subpopulations of TCR/ cells arise sequentially in the chicken thymus
during ontogeny. These two populations (originally known as TCR2 and TCR3) are now
known to represent two distinct V families, V1 and V2, respectively.
The TCR3 mAb reacts with approximately 9% of thymocytes, 15-25% of PBMC and 13% of
splenocytes. This antibody can be used for in ovo suppression of the development of TCR-V2
cells.
315
Feline
Anti-Feline Antigens
CD4
Feline CD4, a member of the immunoglobulin superfamily of cell surface receptors, is a type I
transmembrane glycoprotein that is expressed on the helper/inducer subpopulation of mature T
lymphocytes. In the domestic cat, it is present on approximately 55% of thymocytes, 15% of
splenocytes, 40% of lymph node cells and 25% of peripheral blood lymphocytes. CD4 functions as
an accessory molecule in the recognition of foreign antigens in association with MHC Class II
antigens by T cells.
Clone: 3-4F4 Isotype: IgG1 Mouse (BALB/c)
CD5
Feline CD5, a member of the scavenger receptor family, is expressed on most thymocytes, mature T
lymphocytes and a subpopulation of mature B cells (B-1 cells). CD5 is thought to function in both
cell-cell recognition and signal transduction. MAb f43 is useful as a pan T-cell marker.
Clone: f43 Isotype: IgG1 Mouse (BALB/c)
MAb f43 is useful as a pan T-cell marker.
CD8
Feline CD8, a member of the immunoglobulin superfamily of cell surface receptors, is a type II
transmembrane glycoprotein that is expressed as a heterodimer on the suppressor/cytotoxic
subpopulation of mature T lymphocytes. It is present on 63% of thymocytes, 9% of splenocytes,
20% of lymph node cells and 15% of peripheral blood lymphocytes. CD8 functions as a co-
receptor with MHC Class I-restricted TCRs in antigen recognition.
Clone: fCD8 Isotype: IgG1 Mouse (BALB/c)
316
Porcine
Anti-Porcine Antigens
CD1
Porcine CD1 is a type I transmembrane glycoprotein and a member of the Ig superfamily of cell
surface receptors. It has a domain organization similar to that of MHC Class I molecules and is
expressed in association with 2-microglobulin. CD1 is found on B cells, macrophages, and
immature thymocytes. There is evidence for a role of CD1 in presentation of lipids and peptides to T
cells.
Clone: 76-7-4 Isotype: IgG2a Mouse (BALB/c)
The 76-7-4 mAb was clustered as CD1 at the First International Swine CD Workshop.
CD3
Porcine CD3 is a member of the T-cell receptor-associated CD3 complex. It is found on a
subpopulation of thymocytes and on all pig T lymphocytes.
Clone: BB23-8E6 Isotype: IgG2b Mouse (BALB/c)
MAb BB23-8E6 is mitogenic when presented to peripheral blood mononuclear cells in immobilized
form. The epitope recognized by BB23-8E6 mAb was designated CD3 by the Second
International Swine CD Workshop.
Clone: PPT3 Isotype: IgG1 Mouse (BALB/c)
MAb PPT3 is mitogenic when presented to peripheral blood mononuclear cells in immobilized
form. This mAb was clustered as anti-CD3, at the Second International Swine CD Workshop.
317
Porcine
CD4a
Porcine CD4a is a type I transmembrane glycoprotein and a member of the Ig superfamily of cell
surface receptors. It has a pattern of expression similar to human CD4 and is not expressed on
porcine CD8
+
cells. CD4 is an accessory molecule in the recognition of foreign antigens in
association with MHC Class II molecules by T lymphocytes.
Clone: 74-12-4 (PT4) Isotype: IgG2b Mouse (BALB/c)
The 74-12-4 mAb was clustered as CD4a at the First International Swine CD Workshop. This
antibody can be used to block proliferative responses. It may be used for cytotoxicity assays.
CD8
Porcine CD8, a type I transmembrane glycoprotein, is expressed as either a CD8 homodimer or
a CD8 heterodimer. It is found on most thymocytes, the suppressor/cytotoxic subpopulation of
peripheral T cells and NK cells. CD8 functions primarily as a co-receptor with MHC Class I-
restricted TCRs in antigen recognition.
Clone: 76-2-11 Isotype: IgG2a Mouse (BALB/c)
The 76-2-11 mAb was clustered as CD8 at the First International Swine CD Workshop. This
antibody can be used for cytotoxicity assays.
318
Porcine
CD21
CD21, also known as complement receptor 2, is expressed on all mature circulating B lymphocytes
but not on T lymphocytes or natural killer cells. Low density CD21 has also been reported on
granulocytes and monocytes.
Clone: BB6-11C9.6 Isotype: IgG1 Mouse (BALB/c)
MAb BB6-11C9.6 stains the following percentages of surface immunoglobulin (sIg) positive cells
in porcine lymphoid tissues: 92-96% in peripheral blood; 74-98% in mesenteric lymph nodes; 97
-100% in ileal Peyers patches (ILPP); 93-97% in spleen; and 0% in thymus. The antibody does
not stain B cells in cord blood or spleen from pig fetuses (105 days gestation).
B-Cell Subset
The recognized antigen(s) are found predominantly on surface immunoglobulin (sIg) positive cells in
porcine lymphoid tissues, and on ileal Peyers patches (ILPP) B cells.
Clone: BB6-10A10 Isotype: IgM Mouse (BALB/c)
MAb BB6-10A10 stains the following percentages of surface immunoglobulin (sIg) positive cells in
porcine lymphoid tissues: 11-15% in peripheral blood; 22-23% in mesenteric lymph nodes; 79
-87% in ileal Peyers patches (ILPP); 6-17% in spleen; and 0% in thymus. The antibody appears to
react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of
sIg
low
B cells in lymph nodes.
Monocyte/Granulocyte (SWC3a)
Clone: 74-22-15 Isotype: IgG1 Mouse (BALB/c)
MAb 74-22-15 reacts with > 90% of pig monocytes and granulocytes and < 5% of peripheral
blood lymphocytes. This 74-22-15 mAb was clustered as anti-SWC3a at the First International
Swine CD Workshop.
319
Rabbit
Anti-Rabbit Antigens
IgG
Clone: 2A9 Isotype: IgG1 Mouse (BALB/c)
Light Chain
Clone: SB62a Isotype: IgG2b Mouse (BALB/c)
MHC II (DQ)
It has been reported that the antigen is expressed by the following cells: lymphocytes and
macrophages in the spleen; lymphocytes in the thymic medulla and some cells in the cortex; Kupffer
cells in the liver; and synovial lining cells (i.e., infiltrating macrophages) in inflamed synovium. DQ
transcripts have been detected in bone marrow and spleen and, to a lesser extent, in appendix,
lymph nodes, and other organs.
Clone: 2C4 Isotype: IgG2a Mouse (BALB/c)
MAb 2C4 reacts with RLA-DQ-transfected cells, but not with RLA-DR-transfected cells. In normal
adult rabbits, the antibody reacts with 30-60% of peripheral blood leukocytes.
320
Rabbit
Anti-Rabbit Antigens
MHC II (DR)
It has been reported that the antigen is expressed by the following cells: lymphocytes in the thymic
medulla and some cells in the cortex; periportal cells in the liver; and synovial lining cells (i.e.,
infiltrating macrophages) in inflamed synovium. DR transcripts have been detected in appendix,
bone marrow, spleen and, to a lesser extent, in lymph nodes, lung, liver, and other organs.
Clone: RDR34 Isotype: IgG2b Mouse (BALB/c)
MAb RDR34 reacts with RLA-DR-transfected cells, but not with RLA-DQ-transfected cells. In
normal adult rabbits the antibody reacts with 50-60% of peripheral blood leukocytes and
splenocytes.
321
NOTES
322
---------------------------------------------------- 324-356
Apoptosis / Autophagy
Cell Cycle / Checkpoint Control
Chromatin Regulation/ Nuclear Function
Cytoskeletal Signaling
DNA Damage
Jak/Stat Pathway
Lymphocyte Signaling
MAP Kinase Signaling
Motif Antibodies
NF-k-B Signaling
PI3K/Akt Signaling
Protein Folding/Stability
Related Products
Translational Control
Tyrosine Kinase
Wnt Signaling
323
CD44 is a type I transmembrane glycoprotein that
mediates cell-cell and cell-matrix interaction through its
affinity for hyaluronic acid (HA) and possibly through
other parts of the extracellular matrix (ECM). CD44 is
highly polymorphic, possesses a number of alternative
splice variants and undergoes extensive post-
translational modifications. Increased surface levels of
CD44 are characteristic of T cell activation, and
expression of the protein is upregulated during the
inflammatory response. Interactions between CD44 and
HER2 have been linked to an increase in ovarian
carcinoma cell growth. CD44 interacts with ezrin,
radixin and moesin (ERM), linking the actin
cytoskeleton to the plasma membrane and the ECM.
CD44 is constitutively phosphorylated at Ser325 in
resting cells. Activation of PKC results in
phosphorylation of Ser291, dephosphorylation of
Ser325, disassociation of ezrin from CD44, and
directional motility.
CD44
Flow cytometric analysis of HeLa cells using CD44 (156-3C11) Mouse
mAb (Alexa Fluor 488 Conjugate) (green) compared to a nonspecific
negative control antibody (red). (Data provided by Cell Signaling
Technology Inc.)
Clone: 156-3C11 Isotype: IgG2a Mouse
Detects endogenous levels of total CD44 protein.
Part# Format Size Form Line Status
RUO Cell Lab SC Liquid A88911 50 tests Alexa Fluor* 488
324
Akt (PKB or Rac) plays a critical role in controlling the
balance between survival and apoptosis. This protein
kinase is activated by insulin and various growth and
survival factors, and functions in a wortmannin-
sensitive pathway involving PI3 kinase. Akt is activated
by phospholipid binding and activation loop
phosphorylation at Thr308 by PDK1, and by
phosphorylation within the carboxy-terminus at
Ser473. Akt promotes cell survival by inhibiting
apoptosis through its ability to phosphorylate and
inactivate several targets, including Bad, Forkhead
transcription factors, c-Raf and caspase-9. PTEN
phosphatase is a major negative regulator of the PI3
kinase/Akt signaling pathway. LY294002 is a specific
PI3 kinase inhibitor. One function of Akt is the
regulation of glycogen synthesis through
phosphorylation and inactivation of GSK3 and . Akt
may also play a role in insulin stimulation of glucose
transport. Akt is also involved in cell cycle regulation by
preventing GSK3--mediated phosphorylation and
degradation of cyclin D1, and by negatively regulating
the cyclin-dependent kinase inhibitors p27 KIP and p21
WAF1/CIP1. Akt plays a critical role in cell growth by
directly phosphorylating the mammalian target of
rapamycin, mTOR, but more importantly through
phosphorylation and inactivation of tuberin (TSC2), an
mTOR inhibitor. Inhibition of mTOR stops the protein
synthesis machinery due to inactivation of its effector,
p70 S6 kinase, and activation of the eukaryotic
initiation factor, 4E binding protein 1 (4E-EP1), an
inhibitor of translation.
Akt
Flow cytometric analysis of Jurkat cells (green) using Akt (5G3) Alexa
Fluor 488 Conjugate compared to a nonspecific negative control antibody
(red). (Data provided by Cell Signaling Technology Inc.)
Detects endogenous levels of Akt. This antibody does not cross-react with other related proteins. Detects
endogenous levels of total -catenin protein. Reacts with human, mouse, rat and hamster Akt protein.
325
-catenin is a key downstream effector in the Wnt
signaling pathway. It is implicated in two major
biological processes in vertebrates: early embryonic
development and tumorigenesis. CK1 phosphorylates
-catenin on Ser45. This phosphorylation event primes
-catenin for subsequent phosphorylation by GSK-3.
GSK-3 destabilizes -catenin by phosphorylating it at
Ser33, Ser37 and Thr41. Mutations in these
phosphorylation sites, which result in the stabilization
of -catenin protein levels, have been found in many
tumor cell lines.
-Catenin
Flow cytometric analysis of NCI-H28 cells (blue) and HeLa cells (green)
using -Catenin (L54E2) (Alexa Fluor 488 Conjugate. (Data provided by
Cell Signaling Technology Inc.)
Clone: L54E2 Isotype: IgG1 Mouse
Detects endogenous levels of total -catenin protein. Reacts with human, mouse, rat and monkey total
-catenin protein.
326
Bcl-xL prevents apoptosis through two different
mechanisms: by heterodimerization with an apoptotic
protein to inhibit its apoptotic effect, and by its direct
pore-forming effect on the outer membrane of
mitochondria which helps maintain a normal membrane
state under stress condition. Bcl-xL is phosphorylated
by JNK following treatment with microtubule-damaging
agents such as paclitaxel, vinblastine and nocodazole.
Bcl-xL
Flow cytometric analysis of SEM (blue) and K562 (green) cells using Bcl-
xL (54H6) Alexa Fluor 488 Conjugate compared to nonspecific Rabbit IgG
Isotype Control (Alexa Fluor 488 Conjugate) P/N A88927 (red). (Data
provided by Cell Signaling Technology Inc.)
Clone: 54H6 Isotype: IgG Rabbit
Detects endogenous levels of total Bcl-xL protein. The antibody does not cross-react with other Bcl-2
family members. Reacts with human, mouse, rat and monkey Bcl-xL protein.
327
c-Kit is a member of the subfamily of receptor tyrosine
kinases that includes PDGF, CSF-1 and FLT3/flk-2
receptors. It plays a critical role in activation and
growth in a number of cell types such as hematopoietic
stem cells, mast cells, melanocytes and germ cells.
Upon binding with its ligand, stem cell factor (SCF), c-
Kit undergoes dimerization/oligomerization and
autophosphorylation. Activation of c-Kit results in the
recruitment and tyrosine phosphorylation of
downstream SH2-containing signaling components
including PLC, the p85 subunit of PI3 kinase, SHP2
and CrkL. Molecular lesions that impair the kinase
activity of c-Kit are associated with a variety of
developmental disorders, while mutations that
constitutively activate c-Kit can lead to pathogenesis of
mastocytosis and gastrointestinal stromal tumors.
Tyr719 is located in the kinase insert region of the
catalytic domain. c-Kit phosphorylated at Tyr719 binds
to the p85 subunit of PI3 kinase in vitro and in vivo.
c-Kit
Flow cytometric analysis of Jurkat (blue) and H526 (green) cells using c-Kit
(Ab81) Alexa Fluor 488 Conjugate. (Data provided by Cell Signaling
Technology Inc.)
Clone: Ab81 Isotype: IgG1 Mouse
Detects endogenous levels of total c-Kit protein. It does not cross react with other receptor tyrosine
kinase family members.
328
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is one of
the key executioners of apoptosis, as it is either
partially or totally responsible for the proteolytic
cleavage of many key proteins such as the nuclear
enzyme poly (ADP-ribose) polymerase (PARP).
Activation of caspase-3 requires proteolytic processing
of its inactive zymogen into activated p17 and p12
fragments. Cleavage of caspase-3 requires aspartic
acid at the P1 position.
Cleaved Caspase-3 (Asp175)
Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-
treated (green), using Alexa Fluor 488-conjugate.
Data provided by Cell Signaling Technology, Inc.
Clone: Polyclonal Isotype: Ig Rabbit
Detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from
cleavage adjacent to aspartic acid 175. The antibody does not recognize full length caspase-3 or other
cleaved caspases of p53 only when phosphorylated at Ser15. Reacts with human, mouse, pig, bovine and
rat cleaved caspase-3.
329
Cytochrome c oxidase (COX) is a hetero-oligomeric
enzyme consisting of 13 subunits localized to the inner
mitochondrial membrane. It is the terminal enzyme
complex in the respiratory chain, catalyzing the
reduction of molecular oxygen to water coupled to the
translocation of protons across the mitochondrial inner
membrane to drive ATP synthesis. The three largest
subunits forming the catalytic core are encoded by
mitochondrial DNA while the other smaller subunits,
including COX IV, are nuclear-encoded. Deficiency in
COX activity has been correlated with a number of
human diseases. The COX IV antibody can be used
effectively as a mitochondrial loading control.
COX IV
Flow cytometric analysis of NIH/3T3 (blue) and Jurkat cells (green) using
COX IV (3E11) Alexa Fluor 488 Conjugate. (Data provided by Cell
Signaling Technology Inc.)
Clone: 3E11 Isotype: IgG Rabbit
Detects endogenous levels of total COX IV protein. Reacts with human, rat, monkey and bovine COX IV.
330
Entry of all eukaryotic cells into mitosis is regulated by
activation of cdc2 kinase. Activation of cdc2 is
controlled at several steps including cyclin B1 binding,
phosphorylation of cdc2 at Thr161 and
dephosphorylation of cdc2 at Thr14/Tyr15. The protein
levels of CDK inhibitors and the CDK-associated cyclins
are regulated by phosphorylation, ubiquitination and
degradation, allowing for a stoichiometric regulation of
cell cycle events. Four cyclin B1 phosphorylation sites
(Ser126, 128, 133 and 147) are located in the
cytoplasmic retention signal (CRS) domain and are
thought to regulate the translocation of cyclin B1 to the
nucleus at the G2/M checkpoint. Phosphorylation of
cyclin B1 is required for cdc25C-dependent
dephosphorylation of Tyr15 within cdc2 and subsequent
cdc2/cyclin B1 activation. While cdc2/cyclin B1 itself
can phosphorylate Ser126 and Ser128, polo-like
kinase1 phosphorylates cyclin B1 preferentially at
Ser133 and possibly at Ser147 as well.
Cyclin B1
B1 (V152) Alexa Fluor488 Conjugate versus propidium iodide (DNA
content). (Data provided by Cell Signaling Technology Inc.).
Clone: V152 Isotype: IgG1 Mouse
Detects endogenous levels of cyclin B1 independent of phosphorylation. Reacts with human Cyclin B1
331
Cadherins are a super-family of transmembrane
glycoproteins that contain cadherin repeats of
approximately 100 residues in their extracellular
domain. Cadherins mediate calcium-dependent cell-cell
adhesion and play critical roles in normal tissue
development. The classic cadherin subfamily includes
N-, P-, R-, B- and E-cadherins as well as about 10
other members which are found in adherens junctions
(AJ), a cellular structure near the apical surface of
polarized epithelial cells. The cytoplasmic domain of
classical cadherins interacts with -catenin, -catenin
(also called plakoglobin) and p120 catenin. -catenin
and -catenin associate with -catenin, which links the
cadherin-catenin complex to the actin cytoskeleton.
Unlike - and -catenin p120 regulates cadherin
adhesive activity and trafficking rather than having a
structural role in the junctional complex. E-cadherin is
considered an acting suppressor of invasion and growth
of many epithelial cancers. Recent studies indicate that
cancer cells have up-regulated N-cadherin in addition
to loss of E-cadherin. This change in cadherin
expression is called the cadherin switch. N-Cadherin
cooperates with the FGF receptor, leading to over-
expression of MMP-9 and cellular invasion. In
endothelial cells, VE-cadherin signaling, expression and
localization are correlated with vascular permeability
and tumor angiogenesis.
E-Cadherin
Flow cytometric analysis of HeLa (blue) and MCF-7 cells (green) using E-
Cadherin (24E10) Alexa Fluor 488 Conjugate. (Data provided by Cell
Detects endogenous levels of total E-cadherin protein. The antibody does not cross-react with related
family members, such as N-cadherin. Reacts with human and mouse E-Cadherin protein.
332
Epitope tags are useful for the labeling and detection of
proteins using immunoblotting, immunoprecipitation
and immunostaining techniques. Due to their small
size, they are unlikely to affect the tagged proteins
biochemical properties. The HA tag is derived from an
epitope of the influenza hemagglutinin protein, which
has been extensively used as a general epitope tag in
expression vectors.
HA-Tag
Flow cytometric analysis of COS cells, untransfected (blue) or transfected
with HA-tagged Akt3 (green), using HA-Tag (6E2) Alexa Fluor 488
conjugate. (Data provided by Cell Signaling Technology Inc.)
Detects transfected HA-tagged proteins. The antibody recognizes the HA-tag fused to either the amino or
carboxy terminus of targeted proteins in transfected mammalian cells.
333
HSP70 and HSP90 are molecular chaperones expressed
constitutively under normal conditions to maintain
protein homeostasis and are induced upon
environmental stress. Both HSP70 and HSP90 are able
to interact with unfolded proteins to prevent
irreversible aggregation and catalyze the refolding of
their substrates in an ATP and co-chaperone dependent
manner. HSP70 has a broad range of substrates
including newly synthesized and denatured proteins,
while HSP90 tends to have a more limited subset of
substrates, most of which are signaling molecules.
HSP70 and HSP90 often function collaboratively in a
multi-chaperone system, which requires a minimal set
of co-chaperones: HSP40, Hop and p23. The co-
chaperones either regulate the intrinsic ATPase activity
of the chaperones or recruit chaperones to specific
substrates or subcellular compartments. When the
ubiquitin ligase CHIP associates with the HSP70/HSP90
complex as a cofactor, the unfolded substrates are
subjected to degradation by the proteasome. The
biological functions of HSP70/HSP90 go beyond their
chaperone activity. They are essential for the
maturation and inactivation of nuclear hormones and
other signaling molecules. They also play a role in
vesicle formation and protein trafficking.
HSP70 (D69)
Flow cytometric analysis of HeLa cells using HSP70 (D69) Antibody Alexa
Fluor 647 Conjugate (green) compared to the Rabbit XP (DA1E) IgG
Isotype Control Alexa Fluor 647 Conjugate P/N A88904 (red). (Data
Detects endogenous levels of total HSP70 protein. These antibodies do not cross-react with other HSPs.
Cross react with human, mouse, rat, and monkey.
334
Epitope tags are useful for the labeling and detection of
proteins using immunoblotting, immunoprecipitation
and immunostaining techniques. Due to their small
size, they are unlikely to affect the tagged proteins
biochemical properties. The Myc epitope tag is widely
used to detect expression of recombinant proteins in
bacteria, yeast, insect and mammalian cell systems.
Myc-Tag
Flow Cytometric Analysis of COS cells, untransfected (blue) or transfected
with Myc-tagged Akt (green) using Myc-Tag (9B11) Alexa Fluor 647
conjugate. (Data provided by Cell Signaling Technology Inc.)
Detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused
to either the amino or carboxy terminus of targeted proteins in transfected mammalian cells.
335
Keratins (cytokeratins) are intermediate filament
proteins that are mainly expressed in epithelial cells.
Keratins assemble into filaments, forming heterodimers
of an acidic keratin (or type I keratin, keratins 9 to 23)
and a basic keratin (or type II keratin, keratins 1 to 8).
Keratin isoforms demonstrate tissue- and
differentiation-specific profiles, which make them useful
as biomarkers. Mutations in keratin genes are
associated with skin disorders, liver and pancreatic
diseases, and inflammatory intestinal diseases.
Pan Keratin
Flow cytometric analysis of Jurkat (blue) and MCF-7 cells (green) using
Pan-Keratin (C11) Alexa Fluor 647 Alexa Conjugate. (Data provided by
Cell Signaling Technology Inc.)
Detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react
with other keratins. Cross reacts with human, rat, and monkey.
336
Translation repressor protein 4E-BP1 (also known as
PHAS-1) inhibits cap-dependent translation by binding
to the eIF4E translation initiation factor.
Hyperphosphorylation of 4E-BP1 disrupts this
interaction and cap-dependent translation is activated.
Both the PI3 kinase/Akt pathway and FRAP/mTOR
kinase regulate 4E-BP1 activity. Multiple 4E-BP1
residues are phosphorylated in vivo. While
phosphorylation by FRAP/mTOR on Thr37 and Thr46
does not prevent the binding of 4E-BP1 to eIF4E, it is
thought to prime 4E-BP1 for subsequent
phosphorylation at Ser65 and Thr70.
Phospho-4E-BP1 (Thr37/46)
Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002,
Wortmannin and U0126-treated (blue), using Phospho-4E-BP1 (Thr37/46)
(236B4) Alexa Fluor 488 Conjugate. (Data provided by Cell Signaling
Technology Inc.)
Clone: 236B4 Isotype: IgG Rabbit
Detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody
may cross-react with 4EBP2 and 4E-BP3 when phosphorylated at equivalent sites. Reacts with Phospho
-4E-BP1 (Thr37/46) from human, mouse, rat and monkey.
337
Akt, also referred to as PKB or Rac, plays a critical role
in controlling survival and apoptosis. This protein
survival factors to function in a wortmannin-sensitive
pathway involving PI3 kinase. Akt is activated by
phospholipid binding and activation loop
phosphorylation at Thr308 by PDK1 and by
phosphorylation within the carboxy terminus at Ser473.
The previously elusive PDK2 responsible for
phosphorylation of Akt at Ser473 has been identified as
mammalian target of rapamycin (mTor) in a
rapamycin-insensitive complex with rictor and Sin1. Akt
promotes cell survival by inhibiting apoptosis by
phosphorylating and inactivating several targets,
including Bad, forkhead transcription factors, c-Raf and
caspase-9. PTEN phosphatase is a major negative
regulator of the PI3 kinase/Akt signaling pathway.
LY294002 is a specific PI3 kinase inhibitor. Another
essential Akt function is the regulation of glycogen
synthesis through phosphorylation and inactivation of
GSK-3 and . Akt may also play a role in insulin
stimulation of glucose transport.In addition to its role in
survival and glycogen synthesis, Akt is involved in cell
cycle regulation by preventing GSK-3 mediated
phosphorylation and degradation of cyclin D1 and by
negatively regulating the cyclin dependent kinase
inhibitors p27 Kip and p21 Waf1/CIP1. Akt also plays a
critical role in cell growth by directly phosphorylating
mTOR in a rapamycin-sensitive complex containing
raptor.
Phospho-Akt (Ser473)
Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002 and
wortmannin treated (blue), using Phospho- Akt (Ser473) (193H12) Alexa
Fluor 488 (Data provided by Cell Signaling Technology Inc.)
More importantly, Akt phosphorylates and
inactivates tuberin (TSC2), an inhibitor of
mTOR within the mTOR-raptor complex.
Inhibition of mTOR stops the protein synthesis
machinery due to inactivation of its effector,
p70 S6 kinase and activation of the eukaryotic
initiation factor 4E binding protein 1 (4E-BP1),
an inhibitor of translation.
Detects endogenous levels of Akt only when phosphorylated at Ser473. Cross reacts with human, mouse
and rat.
Clone: D9E Isotype: IgG Rabbit
Detects endogenous levels of Akt only when phosphorylated at Ser473. Cross reacts with human, mouse
and rat Phospho-Akt protein.
338
Akt, also referred to as PKB or Rac, plays a critical role
in controlling survival and apoptosis. This protein
survival factors to function in a wortmannin-sensitive
pathway involving PI3 kinase. Akt is activated by
phospholipid binding and activation loop
phosphorylation at Thr308 by PDK1 and by
phosphorylation within the carboxy terminus at Ser473.
The previously elusive PDK2 responsible for
phosphorylation of Akt at Ser473 has been identified as
mammalian target of rapamycin (mTor) in a
rapamycin-insensitive complex with rictor and Sin1. Akt
promotes cell survival by inhibiting apoptosis by
phosphorylating and inactivating several targets,
including Bad, forkhead transcription factors, c-Raf and
caspase-9. PTEN phosphatase is a major negative
regulator of the PI3 kinase/Akt signaling pathway.
LY294002 is a specific PI3 kinase inhibitor. Another
essential Akt function is the regulation of glycogen
synthesis through phosphorylation and inactivation of
GSK-3 and . Akt may also play a role in insulin
stimulation of glucose transport. In addition to its role
in survival and glycogen synthesis, Akt is involved in
cell cycle regulation by preventing GSK-3 mediated
phosphorylation and degradation of cyclin D1 and by
negatively regulating the cyclin dependent kinase
inhibitors p27 Kip and p21 Waf1/CIP1. Akt also plays a
critical role in cell growth by directly phosphorylating
mTOR in a rapamycin-sensitive complex containing
raptor.
Phospho-Akt (Thr308)
Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or PDGF-
treated (green) using Phospho-Akt (Thr308) (C31E5E) Alexa Fluor 488
Conjugate. (Data provided by Cell Signaling Technology Inc.)
More importantly, Akt phosphorylates and
inactivates tuberin (TSC2), an inhibitor of
mTOR within the mTOR-raptor complex.
Inhibition of mTOR stops the protein synthesis
machinery due to inactivation of its effector,
p70 S6 kinase and activation of the eukaryotic
initiation factor 4E binding protein 1 (4E-BP1),
an inhibitor of translation.
Clone: C31E5E Isotype: IgG Rabbit
Detects endogenous levels of Akt only when phosphorylated at Thr308. Reacts with human, mouse, rat
and monkey phospho-Akt (Thr308).
339
Bcl-2 exerts a survival function in response to a wide
range of apoptotic stimuli through inhibition of
mitochondrial cytochrome c release. It has been
implicated in modulating mitochondrial calcium
homeostasis and proton flux. Several phosphorylation
sites have been identified within Bcl-2 including Thr56,
Ser70, Thr74 and Ser87. It has been suggested that
these phosphorylation sites may be targets of the
ASK1/MKK7/JNK1 pathway, and that phosphorylation
of Bcl-2 may be a marker for mitotic events. Mutation
of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic
activity during glucocorticoid-induced apoptosis of T
lymphocytes. Interleukin 3 and JNK induced Bcl-2
phosphorylation at Ser70 may be required for its
enhanced anti-apoptotic functions.
Phospho-Bcl-2
Flow cytometric analysis of Jurkat cells, untreated (left) or paclitaxel-treated
(right), using Phospho-Bcl-2 (Ser70) (5H2) Alexa Fluor 488 Conjugate
versus propidium iodide (DNA content). The blue inserts represent
propidium iodide staining alone, showing cells in mitotic arrest in the
paclitaxel-treated sample. (Data provided by Cell Signaling Technology
Inc.)
Detects endogenous levels of Bcl-2 only when phosphorylated at Ser70. This antibody does not cross-
react with non phosphorylated Bcl-2 at endogenous levels or with other Bcl-2 family members. Reacts
with human Bcl2 (Ser70).
340
CREB is a bZIP transcription factor that activates target
genes through cAMP response elements. CREB is able
to mediate signals from numerous physiological stimuli,
resulting in regulation of a broad array of cellular
responses. While CREB is expressed in numerous
tissues, it plays a large regulatory role in the nervous
system. CREB is believed to play a key role in
promoting neuronal survival, precursor proliferation,
neurite out growth and neuronal differentiation in
certain neuronal populations. Additionally, CREB
signaling is involved in learning and memory in several
organisms. CREB is able to selectively activate
numerous downstream genes through interactions with
different dimerization partners. CREB is activated by
phosphorylation at Ser133 by various signaling
pathways including Erk, Ca
2+
and stress signaling.
Some of the kinases involved in phosphorylating CREB
at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2.
Phospho-CREB (Ser133)
Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or PDGF
treated (green), using Phospho-CREB (Ser133) (87G3) Alexa Fluor 488
Clone: 87G3 Isotype: IgG Rabbit
Detects endogenous levels of CREB only when phosphorylated at serine 133. The antibody also detects
the phosphorylated form of the CREB-related protein, ATF-1. Reacts with Phospho-CREB (Ser133) from
human, mouse and rat.
341
Both Erk1 and Erk2 (p44 and p42 MAP kinases)
function in a protein kinase cascade that plays a critical
role in the regulation of cell growth and differentiation.
MAP kinases are activated by a wide variety of
extracellular signals including growth and neurotrophic
factors, cytokines, hormones and neurotransmitters.
Activation of MAP kinases occurs through
phosphorylation of threonine and tyrosine (202 and
204 of human MAP kinase or 183 and 185 of rat MAP
kinase) at the sequence T*EY* by a single upstream
MAP kinase kinase (MEK). Both kinases are known to
weakly autophosphorylate on tyrosine.
phospho-Erk1/2 (T202/Y204)
Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or PMA-
treated (green) using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)
(D13.14.4E) XP Alexa Fluor 647 Conjugate. (Data provided by Cell
Clone: E10 Isotype: IgG1 Mouse
Detects endogenous levels of p44 and p42 MAP kinase (Erk1 and Erk2) when dually phosphorylated at
Thr202 and Tyr204. This antibody does not cross-react with the corresponding phosphorylated residues
of either SAPK/JNK or p38 MAP kinase.
Clone: D13.14.4E Isotype: IgG Rabbit
Detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when either dually
phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), or singly phosphorylated at
Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either
JNK/SAPK or p38 MAP kinases. Reacts with human, mouse, rat, monkey, mink, pig, hamster, bovine, D.
melanogaster and zebra fish phospho p44/p42 protein.
342
Modulation of chromatin structure plays an important
role in the regulation of transcription in eukaryotes.
The nucleosome, made up of four core histone proteins
(H2A, H2B, H3 and H4), is the primary building block of
chromatin. The amino-terminal tails of core histones
undergo various post-translational modifications,
including acetylation, phosphorylation and methylation.
These modifications occur in response to cell signaling
stimuli and have a direct effect on gene expression.
DNA damage caused by ionizing radiation, UV-light, or
radiomimetic agents results in rapid phosphorylation of
the histone H2A family member H2A.X at Ser139 by
ATM. Within minutes following DNA damage, Ser139-
phosphorylated H2A.X localizes to sites of DNA damage
at subnuclear foci.
Phospho-Histone H2A.X (Ser139)
Flow cytometric analysis of Jurkat cells, untreated (green) or etoposide-
treated (blue), using Phospho-Histone H2A.X (Ser139) (Clone 20E3) (Alexa
Fluor 488 Conjugate). (Data provided by Cell Signaling Technology Inc.).
Detects endogenous levels of H2A.X only when phosphorylated at serine 139.
343
Modulation of chromatin structure plays an important
role in the regulation of transcription in eukaryotes.
The nucleosome, made up of four core histone proteins
(H2A, H2B, H3 and H4), is the primary building block of
chromatin. The amino-terminal tails of core histones
undergo various post translational modifications,
including acetylation, phosphorylation, methylation and
ubiquitination. These modifications occur in response to
various stimuli and have a direct effect on the
accessibility of chromatin to transcription factors and,
therefore, on gene expression. In most species, histone
H2B is primarily acetylated at lysines 5, 12, 15 and 20.
Histone H3 is primarily acetylated at lysines 9, 14, 18
and 23. Acetylation at lysine 9 appears to have a
dominant role in histone deposition and chromatin
assembly in some organisms. Phosphorylation at Ser10
of histone H3 is tightly correlated with chromosome
condensation during both mitosis and meiosis.
phospho-Histone H3 (Ser10)
Flow cytometric analysis of THP1 cells treated with paclitaxel, using
Phospho-Histone H3 (Ser10) Antibody Alexa Fluor 488 conjugate versus
propidium iodide (DNA content). The box indicates phospho-histone H3
positive cells. (Data provided by Cell Signaling Technology Inc.)
Detects endogenous levels of histone H3 only when phosphorylated at serine 10. The antibody does not
cross-react with other phosphorylated histones or with acetylated histones. Cross reacts with human and
mouse.
344
In response to cytokines, stress and chemotactic
factors, MAP kinase-activated protein kinase 2
(MAPKAPK-2) is rapidly phosphorylated and activated.
It has been shown that MAPKAPK-2 is a direct target of
p38 MAPK. Multiple residues of MAPKAPK-2 are
phosphorylated in vivo in response to stress. However,
only four of the residues: Thr25, Thr222, Ser272 and
Thr334 are phosphorylated by p38 MAPK in an in vitro
kinase assay. Phosphorylation at Thr222, Ser272 and
Thr334 appears to be essential for the activity of
MAPKAPK-2. Thr25 is phosphorylated by p42 MAPK in
vitro, but is not required for the activation of MAPKAPK
-2.
phospho-MAPKAPK-2 (Thr334)
Flow cytometric analysis of THP-1 cells, untreated (blue) or anisomycin-
treated (green), using Phospho-MAPKAPK-2 (Thr334) (27B7) Alexa Fluor
647 Conjugate. (Data provided by Cell Signaling Technology Inc.).
Clone: 27B7 Isotype: IgG Rabbit
The anti-Phospho-MAPKAPK-2 (Thr334) monoclonal antibody (clone 27B7) detects endogenous levels of
MAPKAPK-2 protein only when phosphorylated at threonine 334. It reacts with human, mouse, rat and
monkey.
345
Transcription factors of the nuclear factor B (NF-B)
/Rel family play a pivotal role in inflammatory and
immune responses. There are five family members in
mammals; RelA, c-Rel, RelB, NF-B1 (p105/p50), and
NF-B2 (p100/p52). p105 and p100 are proteolytically
processed by the proteasome to produce p50 and p52,
respectively. The p50 and p52 products form dimeric
complexes with Rel proteins, which are then able to
bind DNA and regulate transcription. In unstimulated
cells, NF-B is sequestered in the cytoplasm by its
inhibitory proteins, the IBs. NF-B activating agents
can induce the phosphorylation of IBs, which targets
them for rapid degradation through a ubiquitin-
proteasome pathway, releasing NF-B to enter the
nucleus and regulate gene expression. Processing of
NF-B2 is regulated by IKK1 (IKK-), which triggers
the phosphorylation and processing to p52, which can
then undergo nuclear translocation.
phospho-NF-B p65 (Ser536)
Flow cytometric analysis of THP-1 cells, untreated (blue) or TNF- treated
(green), using Phospho-NF-B p65 (Ser536) (93H1) Alexa Fluor 647
Detects NF-kB p65 only when phosphorylated at serine 536. It does not cross-react with the p50 subunit
or other related proteins. Cross reacts with human, mouse, and rat
346
p38 MAP kinase (MAPK), also called RK or CSBP, is the
mammalian orthologue of the yeast HOG kinase and
participates in a signaling cascades controlling cellular
responses to cytokines and stress. Four isoforms of p38
MAP kinase, , , and , have been identified so far.
Like the SAPK/JNK pathway, p38 MAP kinase is
activated by a variety of cellular stresses including
osmotic shock, inflammatory cytokines,
lipopolysaccharides (LPS), UV light and growth factors.
MKK3 and SEK activate p38 MAP kinase by
phosphorylation at threonine 180 and tyrosine 182.
Activated p38 MAP kinase has been shown to
phosphorylate and activate MAPKAP kinase 2 and to
phosphorylate the transcription factors ATF-2, Max and
MEF2.
phospho-p38 MAPK (Thr180/Tyr182)
Flow cytometric analysis of THP-1 cells, untreated (blue) or anisomycin-
treated (green), using Phospho-p38 MAPK (Thr180/Tyr182) (28B10)
Mouse mAb (Alexa Fluor 488 Conjugate) compared to a nonspecific control
antibody (red). (Data provided by Cell Signaling Technology Inc.).
Clone: 28B10 Isotype: IgG1 Mouse
Detects endogenous levels of p38 MAP kinase only when dually phosphorylated at Thr180 and Tyr182.
This antibody does not appreciably cross-react with the corresponding phosphorylated forms of either
p44/42 MAPK (Erk1/2) or SAPK/JNK.
347
The p53 tumor suppressor protein plays a major role in
cellular response to DNA damage and other genomic
aberrations. Activation of p53 can lead to either cell
cycle arrest and DNA repair or apoptosis. p53 is
phosphorylated at multiple sites in vivo and by several
different protein kinases in vitro. DNA damage induces
phosphorylation of p53 at Ser15 and Ser20 and leads
to a reduced interaction between p53 and its negative
regulator, oncoprotein MDM2. MDM2 inhibits p53
accumulation by targeting it for ubiquitination and
proteasomal degradation. p53 can apparently be
phosphorylated by ATM, ATR and DNA-PK at Ser15 and
Ser37. Phosphorylation impairs the ability of MDM2 to
bind p53, promoting both the accumulation and
activation of p53 in response to DNA damage. Chk2
and Chk1 can phosphorylate p53 at Ser20, enhancing
its tetramerization, stability and activity. p53 is
phosphorylated at Ser392 in vivo and by CAK in vitro.
Phosphorylation of p53 at Ser392 is altered in human
tumors and has been reported to influence the growth
suppressor function, DNA binding and transcriptional
activation of p53. p53 is phosphorylated at Ser6 and
Ser9 by CK1 and CK1 both in vitro and in vivo.
Phosphorylation of p53 at Ser46 regulates the ability of
p53 to induce apoptosis. Acetylation of p53 is mediated
by p300 and CBP acetyltransferases. Inhibition of
deacetylation suppressing MDM2 from recruiting
HDAC1 complex by p19 (ARF) stabilizes p53.
Acetylation appears to play a positive role in the
accumulation of p53 protein in stress response.
Following DNA damage, human p53 becomes
acetylated at Lys382 (Lys379 in mouse) in vivo to
enhance p53-DNA binding. Deacetylation of p53 occurs
through interaction with the SIRT1 protein, a
deacetylase that may be involved in cellular aging and
the DNA damage response.
Phospho-p53 (Ser15)
Flow cytometric analysis of HT29 cells, untreated (blue) or UV treated
(green), using Phospho-p53 (Ser15) (16G8) Alexa Fluor 488 Conjugate.
(Data provided by Cell Signaling Technology Inc.)
Detects endogenous levels of p53 only when phosphorylated at Ser15. The antibody does not cross-react
with p53 phosphorylated at other sites. Reacts with Phospho-CREB (Ser133) from human, mouse and
rat.
348
To effectively promote growth and cell division in a
sustained manner, growth factors and mitogens must
upregulate translation. Growth factors and mitogens
induce the activation of p70 S6 kinase, which in turn
phosphorylates the S6 ribosomal protein.
Phosphorylation of S6 correlates with an increase in
translation, particularly of mRNAs with an
oligopyrimidine tract in their 5 untranslated regions.
This group of mRNAs (5TOP) encodes proteins involved
in cell cycle progression and proteins that are part of
the translational machinery, such as ribosomal proteins
and elongation factors. The main in vivo S6 ribosomal
protein phosphorylation sites, including Ser235,
Ser236, Ser240 and Ser244, are located within a small
19 amino acid region in the S6 carboxy-terminus.
phospho-S6 Ribosomal Protein (Ser235/236)
Flow cytometric analysis of K562 cells, untreated (green) or STI571-treated
(blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP
Alexa Fluor 647 Conjugate. (Data provided by Cell Signaling Technology
Inc.)
Clone: D57.2.2E Isotype: IgG Rabbit
Detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and 236. Cross
reacts with human, mouse, rat, monkey and s. cerevisiae.
Clone: 2F9 Isotype: IgG Rabbit
The anti-PhosphoS6 Ribosomal Protein (Ser235/236) Rabbit mAb (Clone 2F9) detects endogenous levels
of ribosomal protein S6 only when phosphorylated at serine 235 and 236.
349
The stress-activated protein kinase/Jun-amino-terminal
kinase SAPK/JNK is potently and preferentially
activated by a variety of environmental stresses
including UV and gamma radiation, ceramides,
inflammatory cytokines and in some instances, by
growth factors and GPCR agonists. As with the other
MAPKs, the core signaling unit is composed of a
MAPKKK, typically MEKK1-MEKK4, or by one of the
mixed lineage kinases (MLKs), which phosphorylate
and activate MKK4/7. Upon activation, MKKs
phosphorylate and activate the SAPK/JNK kinase.
Stress signals are delivered to this cascade by small
GTPases of the Rho family (Rac, Rho, cdc42). Both
Rac1 and cdc42 mediate the stimulation of MEKKs and
MLKs. Alternatively, MKK4/7 can be activated in a
GTPase independent mechanism via stimulation of a
germinal center kinase(GCK) family member. There are
three SAPK/JNK genes each of which undergoes
alternative splicing resulting in numerous isoforms.
SAPK/JNK, when active as a dimer, can translocate to
the nucleus and regulate transcription through its
effects on c-Jun, ATF-2 and other transcription factors.
phospho-SAPK/JNK (Thr183/Tyr185)
Flow cytometric analysis of THP1 cells, untreated (blue) or anisomycin-
treated (green) using Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Alexa
Fluor 647 conjugate compared to a nonspecific control antibody (red). (Data
Clone: G9 Isotype: IgG1 Mouse
Detects endogenous levels of p46 and p54 SAPK/JNK dually phosphorylated at Thr183 and Tyr185. This
antibody does not recognize endogenous levels of phosphorylated p44/42 MAPK or p38 MAP kinase.
Reacts with human, mouse, hamster and rat phospho-SAPK/JNK (Thr183/Tyr185).
350
Stat1, while activated in response to a large number of
ligands, appears to be essential for responsiveness to
IFN- and IFN-. Phosphorylation of Stat1 at Tyr701
induces Stat1 dimerization, nuclear translocation and
DNA binding. Stat1 has two isoforms, Stat1 (91 kDa)
and the splice variant Stat1 (84 kDa). In most cells,
both isoforms are activated by IFN-, but only Stat1 is
activated by IFN-. Stat1 has been found to be
inappropriately activated in many tumors. In addition
to tyrosine phosphorylation, Stat1 is phosphorylated
through a p38 mitogen-activated protein kinase
(MAPK)-dependent pathway at Ser727 in response to
IFN- and other cellular stresses. Serine
phosphorylation may be required for the maximal
induction of Stat1 mediated gene activation.
Phospho-Stat1 (Tyr701)
Flow cytometric analysis of Jurkat cells, untreated (blue) or IFN- treated
(green), using Phospho-Stat1 (Tyr701) (58D6) Alexa Fluor 488 Conjugate.
(Data provided by Cell Signaling Technology Inc.)
Clone: 58D6 Isotype: IgG Rabbit
Detects endogenous levels of Stat1 only when phosphorylated at Tyr701. The antibody detects
phosphorylated Tyr701 of p91 Stat1 and also the p84 splice variant. It does not cross-react with the
corresponding phospho-tyrosines of other Stat proteins. Reacts with human, rat and mouse phospho-
Stat1 protein.
351
Stat3 is a key signaling molecule for many cytokines
and growth-factor receptors and is required for murine
fetal development. Additionally, Stat3 is constitutively
activated in a number of human tumors and possesses
oncogenic potential and anti-apoptotic activities. Stat3
is activated by phosphorylation at Tyr705, which
induces dimerization, nuclear translocation and DNA
binding. Transcriptional activation seems to be
regulated by phosphorylation at Ser727 via the MAPK
or mTOR pathway. Stat3 isoform expression appears to
reflect biological function: the relative expression levels
of Stat3 (86 kDa) and Stat3 (79 kDa) depend on cell
type, ligand exposure or maturation stage of the cells.
It is notable that Stat3 lacks the serine
phosphorylation site within the carboxy-terminal
transcriptional activation domain.
phospho-Stat3 (Tyr 705)
Flow cytometric analysis of Jurkat cells, untreated (blue) or IFN- treated
(green), using Phospho-Stat3 (Tyr705) (D3A7) XP Alexa Fluor 647
Clone: D3A7 Isotype: IgG Rabbit
Detects endogenous levels of Stat3 only when phosphorylated at Tyr705. This antibody does not cross-
react with phospho-EGFR or with corresponding phospho-tyrosines of other Stat proteins. Cross reacts
with human, mouse, rat, and monkey.
352
Since the original observations that protein tyrosine
kinases (PTKs) play critical roles in oncogenic
transformation, it has become clear that both protein
tyrosine kinases and phospho-tyrosine phosphatases
control a wide range of critical biological processes.
Antibodies specific for phospho-tyrosine have been
invaluable reagents in these studies. The present anti-
phospho-tyrosine monoclonal antibody (clone P-Tyr
-100) shows exceptional sensitivity for studying
tyrosine phosphorylation and monitoring tyrosine
kinase activity in high throughput drug discovery.
phospho-Tyrosine
Flow cytometric analysis of K562 cells, untreated (green) or Gleevec-
treated (blue), using Phospho-Tyrosine Mouse (P-Tyr-100) (Alexa Fluor
488 Conjugate) compared with a non-specific negative control antibody
(red).. (Data provided by Cell Signaling Technology Inc.).
Clone: P-Tyr-100 Isotype: IgG1 Mouse
Phospho-Tyrosine Clone P-Tyr-100 is a high affinity antibody. P-Tyr-100 does not cross-react with
peptides containing phospho-serine or phospho-threonine.
353
PU.1 is a member of the Ets family of transcription
factors and activates target genes through the purine-
rich PU-box. PU.1 plays a pivotal role in the
differentiation of myeloid cells and lymphocytes and is
expressed in several hematopoietic cells including B
lymphocytes, macrophages, neutrophils, mast cells,
early erythroid cells and megakaryocytes. The
concentration of PU.1 is critical for both the
determination of hematopoietic cell lineage and the
regulation of differentiation versus stem cell
proliferation. In addition, PU.1 activity is influenced by
phosphorylation and interactions with other
hematopoietic transcription factors. Phosphorylation of
PU.1 at Ser146 by CK2 promotes binding to IRF4 and
synergistic activation through the immunoglobulin K 3
enhancer. Treatment of pro-B cells with IL-3 leads to
phosphorylation of PU.1 at Ser140, resulting in
increased PU.1 activity and activation of the anti-
apoptotic gene MCL-1. GATA1 binding blocks PU.1
activity during erythroid cell development. Over-
expression of PU.1 resulting from proviral insertion
during Friend virus infection can induce
erythroleukemia, while reduced expression has been
associated with acute myeloid leukemia.
PU.1
Flow Cytometric Analysis of MCF-7 cells (blue) or THP-1 cells (green)
using PU.1 (9G7) Alexa Fluor 488 conjugate. (Data provided by Cell
Clone: 9G7 Isotype: IgG Rabbit
Detects endogenous levels of total PU.1 protein. This antibody does not cross react with other Ets family
members. Cross reacts with mouse and human PU.1.
354
Survivin is a 16 kDa anti-apoptotic protein highly
expressed during fetal development and cancer cell
malignancy. By binding and inhibiting caspase-3,
survivin controls the checkpoint in the G2/M-phase of
the cell cycle by inhibiting apoptosis and promoting cell
division. This regulatory process requires the
phosphorylation of survivin at Thr34 by p34 cdc2
kinase. Gene targeting using a Thr34 phosphorylation-
defective survivin mutant, as well as antisense survivin,
has been shown to inhibit tumor growth.
Survivin
Flow Cytometric Analysis of untreated (green) and etoposide treated (blue)
Jurkat cells using Survivin (71G4B7) Alexa Fluor 488 conjugate showing a
decrease in expression of survivin in apoptotic cells(Data provided by Cell
Clone: 71G4B7 Isotype: IgG Rabbit
Detects endogenous levels of total survivin protein. Cross reacts with mouse, human and rat.
355
Zap-70, a Syk family protein tyrosine kinase expressed
in T and NK cells, plays a critical role in mediating T cell
activation in response to T cell receptor (TCR)
engagement. Following TCR engagement, Zap-70 is
rapidly phosphorylated on several tyrosine residues,
presumably by two mechanisms: autophosphorylation
and transphosphorylation by the Src family tyrosine
kinase, Lck. Tyrosine phosphorylation of Zap-70
correlates with its increased kinase activity and
downstream signaling events. In patients with chronic
lymphocytic leukemia (CLL), total Zap-70 expression
was shown to be correlated with disease progression
and survival.
ZAP-70
Flow cytometric analysis of Ramos B (blue) and Jurkat T (green) cells using
Zap-70 (136F12) Alexa Fluor 647 Conjugate. (Data provided by Cell
Clone: 136F12 Isotype: IgG Rabbit
Detects endogenous levels of total Zap-70 protein.
RUO Cell Lab Liquid A88883 50 tests Alexa Fluor* 647
356
Tetramers ---------------------------------------- 358-374
iTopia --------------------------------------------- 375-376
Streptamer --------------------------------------- 377-385
357
Tetramers
Investigation of the antigen specific T cell response begins with peptide epitopes. Understanding the
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Prior to the development of class I and class II MHC tetramers, techniques for the isolation and
identification of antigen-specific T cells were tedious, imprecise, lacked reproducibility, and inferred the
epitope specificity of a T cell based on a functional test. A complex of 4 MHC-peptide pairs and a
streptavidin fluorochrome, MHC Tetramers directly detect antigen-specific T cells at the single cell level
based on the structural specificity of the T cell. Functional analysis of the Tetramer positive cells may then
be performed providing both the structural specificity and functional profile of the antigen-specific T cell
response.
With our unparalleled iTAg MHC Tetramer portfolio for Class I and Class II molecules, antigen-specific T
cells can be accurately detected at the single cell level. The quantitation of the T cell response provided by
Beckman Coulters iTAg MHC Tetramers is pivotal for vaccine and drug development, as well as clinical trial
monitoring.
Antigen-specific CD8
+
T cells are enumerated directly from patient samples without the need for cell
stimulation.
The only method to detect antigen-specific CD8
+
T cells ex vivo.
Immune responses are targeted to the specific allele(s).
Time from sample isolation to readout is within hours, not days.
Quantify Tetramer positive CD8
+
T cells for further functional characterization.
Non-specific binding is inherent in immunologic assays. To greatly reduce non-specific binding, Class I
iTAg MHC Tetramers specific to human alleles have a proprietary mutation in the -3 domain (see
histograms).
Class I iTAg MHC Tetramers
Premium iTAg MHC Tetramers -shipped within 3 days. Select iTAg MHC Tetramers - shipped within 3 to 30 days.
All Custom Products are usually shipped within 30 to 60 days.
Use Part Number designated "CP" when peptide is customer-supplied. Use Part Number designated "IP" when Beckman Coulter supplies peptide.
All "IP" products include a peptide acquisition fee, Part Number T05008.
358
Tetramers
Human Class I Allele iTAg MHC Tetramers (in Stock)
A) Infectious Disease
CMV
HLA-A*0101
CMV (VTEHDTLLY)
Part # Status Fluorochrome Size Category
T01045 PE 50 tests RUO Premium
T01050 APC 50 tests RUO Select
CMV
HLA-A*0201
CMV PP65 (NLVPMVATV)
CMV
HLA-A*2402
CMV (FTSQYRIQGKL)
CMV
HLA-A*2402
CMV (QYDPVAALF)
CMV
HLA-B*0702
CMV (TPRVTGGGAM)
359
Tetramers
CMV
HLA-B*0801
CMV (ELRRKMMYM)
CMV
HLA-B*3501
CMV (IPSINVHHY)
EBV
HLA-A*0201
EBV (GLCTLVAML)
HBV
HLA-A*0201
HBV core (FLPSDFFPSV)
T01018 PE 50 tests RUO Select
HBV
HLA-A*2402
HBV core (EYLVSFGVW)
HBV
HLA-A*2402
HBV pol (KYTSFPWLL)
360
Tetramers
HIV
HLA-A*0201
HIV gag (SLYNTVATL)
HIV
HLA-A*0201
HIV pol (ILKEPVHGV)
HIV
HLA-B*5701
HIV gag (KAFSPEVIPMF)
T01069 APC 50 tests RUO Premium
HPV
HLA-A*0201
HPV (YMLDLQPET)
HTLV-1
HLA-A*0201
HTLV-1 (LLFGYPVYV)
Influenza
HLA-A*0201
Influenza-M1 (GILGFVFTL)
361
Tetramers
NY-ESO-1
HLA-A*0201
NY-ESO-1 (SLLMWITQC)
B) Cancer
Gp 100 Antigen
HLA-A*0201
Gp 100 (ITDQVPFSV)
Gp 100 Antigen
HLA-A*0201
Gp100 (IMDQVPFSV)
Her-2/neu Antigen
HLA-A*0201
Her-2/neu (KIFGSLAFL)
Her-2/neu Antigen
HLA-A*0201
Her-2/neu (RLLQETELV)
362
Tetramers
Mart-1 Antigen
HLA-A*0201
Mart-1 (ELAGIGILTV)
PR-1 Antigen
HLA-A*0201
PR-1 (VLQELNVTV)
Tyrosinase
HLA-A*0201
Tyrosinase (YMDGTMSQV)
C) Negative Tetramers
The iTAg MHC Class I Human Negative Tetramer is intended to be prepared and analyzed in
parallel with peptide specific iTAg MHC Class I Human Tetramers. The reagent may be used
to assess the level of background PE fluorescence using standard flow cytometry
methodologies.The iTAg MHC Class I Negative Tetramer conjugated to SA-PE may be used to
assess the level of background fluorescence and nonspecific binding for all iTAg MHC Class I
Human Tetramers conjugated to SA-PE.
The iTAg MHC Negative Tetramer has no known specificity and does not recognize human
CD8
+
T cells of any HLA allele. The HLA molecule in this reagent has been modified to
minimize CD8-mediated binding.
Negative Tetramer
363
Tetramers
Human Class I Allele iTAg MHC Tetramers (Custom)
HLA-A*0101
T02002 PE 50 tests RUO CP
T02046 APC 50 tests RUO CP
T02003 PE 50 tests RUO IP
T02047 APC 50 tests RUO IP
HLA-A*0201
HLA-A*0301
HLA-A*1101
HLA-A*2402
364
Tetramers
HLA-B*0702
HLA-B*0801
HLA-B*1501
HLA-B*2705
HLA-B*3501
365
Tetramers
HLA-B*5701
Non-human Allele iTAg Tetramers
Murine iTAg Tetramers
Gp 100
H-2 Db
Gp 100 (KVPRNQDWL)
HIV
H-2 Kd
HIV gag (AMQMLKETI)
HPV
H-2 Db
HPV16 (RAHYNIVTF)
LCMV
H-2 Db
LCMV (KAVYNFATC)
366
Tetramers
LCMV
H-2 Kb
LCMV (AVYNFATC)
LCMV
H-2 Kb
LCMV (AVYNFATCGI)
LCMV
H-2 Kb
LCMV (FQPQNGQFI)
LCMV
H-2 Kb
LCMV (ISHNFCNL)
LCMV
H-2 Kb
LCMV (LEYDFNKL)
LCMV
H-2 Kb
LCMV (SGVENPGGYCL)
367
Tetramers
LCMV
H-2 Kb
LCMV (YTVKYPNL)
OVA
H-2 Kb
OVA (SIINFEKL)
RSV
H-2 Kd
RSV M2 (SYIGSINNI)
TRP2
H-2 Kb
TRP2 (SVYDFFVWL)
Rhesus Macaque iTAg Tetramers
SIV
Mamu-A*01
SIV gag (CTPYDINQM)
SIV
Mamu-A*01
SIV gag (CTPYDINQM)
368
Tetramers
Non-human Allele iTAg Tetramers (Custom)
Murine iTAg Tetramers
H-2 Db
H-2 Kb
H-2 Kd
Rhesus Macaque iTAg Tetramers
Mamu-A*01
369
Tetramers
CD4
+
T cells play a role in many diseases, specifically autoimmune disease. Analysis of these cells has
long been hampered by lack of a suitable assay. Beckman Coulters recent advances in Class II iTAg
MHC Tetramer manufacturing processes have met this need. Beckman Coulter is committed to
continually introduce new Class II alleles to meet clinical research needs.
Class II iTAg MHC Tetramers enumerate antigen specific CD4
+
T cells directly from patient samples ex
vivo, or following in vitro stimulation (see histograms).
The only method to detect antigen-specific CD4
+
T cells ex vivo.
Immune responses are targeted to the specific allele(s).
Quantify Tetramer positive CD4
+
T cells for further functional characterization.
Staining Whole Blood ex vivo. Example of
direct whole blood staining with EBV Class II
iTAg MHC Tetramer. Figure 2a-Irrelevant
Tetramer. Figure 2b-Positive Tetramer.
Class II iTAg MHC Tetramers
Human Class II Allele iTAg MHC Tetramers (Custom)
DPB1*0401 (DP4)
T02062 PE 500 L RUO CP
T02063 PE 500 L RUO IP
DRB1*0101
T02052 PE
500 L
RUO CP
T02053 PE
500 L
RUO IP
DRB1*0301 (DR3)
T02066 PE 500 L RUO CP
T02067 PE 500 L RUO IP
370
Tetramers
DRB1*0401
T02050 PE
500 L
RUO CP
T02051 PE
500 L
RUO IP
DRB1*1501
T02058 PE
500 L
RUO CP
T02059 PE
500 L
RUO IP
371
Tetramers
iTAg MHC Tetramer CMV Multi-Allele (6 vials) Sales Group.
The sales group contains the following CMV specific Tetramers and the Negative Tetramer:
iTAg MHC A*0101 Class I, peptide sequence VTEHDTLLY (CMV pp50 antigen)
iTAg MHC A*0201 Class I, peptide sequence NLVPMVATV (CMV pp65 antigen)
iTAg MHC B*0702 Class I, peptide sequence TPRVTGGGAM (CMV pp65 antigen)
iTAg MHC B*0801 Class I, peptide sequence ELRRKMMYM (CMV pp65 antigen)
iTAg MHC B*3501 Class I, peptide sequence IPSINVHHY (CMV IE-1 antigen)
iTAg MHC Negative Tetramer
To obtain the absolute count of CMV specific T
cells, the sample is divided into two tubes. The
results from the no wash, flowcount stained tube
(top) is combined with the washed, tetramer
stained tube (bottom) to obtain the absolute
concentration of CMV specific T cells par L.
iTag MHC Tetramer CMV Assay
Multi Allele
CMV Tetramers
372
Tetramers
Advanced tetramer analysis combined with intracellular cytokine staining (ICC) provides both
quantitative and functional immune response analysis using a single sample. Beckman Coulter's iTAg
MHC Tetramer IFN- Kit measures functional status of the immune response targeted to specific alleles
and antigens. This powerful combination also allows you to stratify specific and non-specific cytokine
generation, providing more accurate immune response assessment. The protocol allows for the
addition of antibodies for further characterization of specific subpopulations, still using a single sample.
Eliminate possible overestimation of total immune response to a particular antigen, which can occur
with ICC staining alone.
Provides complete and accurate visualization of antigen specificity and IFN- production.
Requires less sample material than if assays were performed individually.
The kit, designed for 50 tests, is composed of anti-IFN- FITC; Lyse reagent; Fixative reagent;
Permeabilization reagent; and, Brefeldin A.
iTAg MHC Tetramer IFN- Kit: this
histogram demonstrates subdivision of
T cells into quadrants based on
function and antigen specificity.
iTAg MHC Tetramer IFN- Kit
T08005 - 50 tests RUO -
373
Tetramers
A sequential gating approach with dual parametric histograms to resolve the antigen-specific T cell
population.
Improves signal-to-noise.
Increases resolution of rare events.
For use with all Class I Human iTAg MHC Tetramers.
The kit, designed for 100 tests, is composed of:
2 vials of CD8-FITC; 1 vial CD4-PC5; 1 vial CD19-PC5 and 1 vial CD13-PC5.
Dot plot 1 represents the iMASC gate (A) which
excludes CD4, CD13 and CD19 from the
analysis. Dot plot 2 is generated from region A.
Region B is drawn, based on morphology, and
excludes debris from the gate. Dot plot 3 is
generated from regions A and B. Region C is
drawn to include CD8
+
T cells only. Dot plot 4
is generated from regions A, B and C. Region D
represents antigen specific CD8
+
/iTAg Tetramer
positive T cells.
iMASC Antibody Gating Kit
Antibody Gating kit
T09004 - 100 tests RUO -
iTAg MHC Tetramer Lyse and Fixative preserve the integrity of the cells.
Clear definition of lymphocyte, monocyte and granulocyte populations and less debris in sample
populations.
iTAg MHC Tetramer Lyse and Fixative
Lysing Solution
T08002 - 100 mL RUO -
Fixative Solution
T08003 - 10 mL RUO -
374
Thorough understanding of the antigen-specific T cell response requires the analysis of numerous epitopes
across multiple alleles. The iTopia system from Beckman Coulter provides high throughput T cell epitope
discovery across eight human alleles. In contrast to in silico methods, the iTopia system provides
comprehensive epitope information based on experimental data, not mathematical predictions, and requires no
patient material. A comprehensive report is provided containing all putative epitopes in order of probable
immunogenicity. Confirmation and quantitation of the T cell response to each epitope can then be carried out
utilizing MHC Tetramer technology.
In order to mount an immune response, immunogenic proteins are transported to, and internalized by antigen
presenting cells. Peptides from the original protein then bind to MHC molecules and are expressed on the cells
surface in the context of MHC. Subsets of these bound peptides are capable of generating an immune response
and are termed epitopes.
The science behind the process is the assembly of properly folded MHC complexes in conjunction with Beta 2
microglobulin and peptides in a microwell plate assay. A fluorescently labeled antibody binds to the properly
folded MHC complex. Utilizing this assay enables accurate quantitation of peptide binding, affinity and off-rate.
The included iTopia software then utilizes this information providing a systematic, data based ranking of
epitope candidates which can be prioritized for further functional studies.
iTopia can quickly and accurately identify the most common immunogenic regions of the peptide/protein,
bringing efficiency and specificity to vaccine design. The information obtained from the iTopia system can then
be applied to clinical trial monitoring by identifying the vaccine that is most likely to protect and/or the
individuals most likely to be protected. It may also be instrumental in developing more potent vaccines and
immunotherapies that can be used in treating large patient populations.
iTopia
The iTopia Epitope Discovery System
The iTopia Epitope Discovery System, which mimics in vivo peptide binding reactions, helps
researchers advance important candidates more quickly through the development process.
Identify allele specific epitopes that can be used in clinical trials together important immune
response information
Identify vaccine candidates not providing the desired immune response levels earlier in the
process, allowing redesign of clinical trials or elimination of candidate vaccine from further study
Identify epitopes within the protein that may potentially be enhanced or modified to produce a
stronger immune response
Utilizes no patient materials
Automation friendly 96-well plate format
iTopia
375
iTopia
Human Class I
iTopia Kit
Part #
Status
HLA-A*0101
T06100 96 tests RUO
HLA-A*0201
T06101 96 tests RUO
HLA-A*0301
T06102 96 tests RUO
HLA-A*1101
T06103 96 tests RUO
HLA-A*2402
T06104 96 tests RUO
HLA-B*0702
T06105 96 tests RUO
HLA-B*0801
T06106 96 tests RUO
HLA-B*1501
T06107 96 tests RUO
Accessories
iTopia Epitope Discovery System Customer Package
Part #
Status
Prerequisite Guide & Customer Guide
T06001 RUO
iTopia Start up Kit
Part #
Status
T06000 RUO
iTopia System Software
Part #
Status
T06210 RUO
376
The Streptamer technology is a novel method to isolate and characterize functional antigen-specific T-
cells. Streptamer enable for the first time a reversible, antigen-specific staining of T-cells, leaving the
isolated T-cells phenotypically and functionally indistinguishable from untreated cells. Streptamer-isolated
T-cells may offer new strategies for treating incurable diseases including viral infections and cancer by
adoptive T-cell transfer. In addition, the technology is likely to advance basic T-cell research as an
essential prerequisite for novel immunological medicines.
Streptamer
The Streptamer approach is based on a reversible staining procedure that utilizes IBA's core Strep-tag
technology to tag MHCs which are non-covalently attached to fluorescently labeled Strep-Tactin or
Strep-Tactin coupled to magnetic beads. Highly stable streptavidin derivatives are optimized for
binding Strep-tag, a short peptide sequence with affinity for the biotin-binding site of Strep-Tactin.
MHC/peptide complexes fused with a specifically engineered Strep-tag sequence are generated and
multimerized in the presence of Strep-Tactin. This complex - called "Streptamer" - is ideally suited to
bind and isolate cognate T-cells by fluorescence activated cell sorting or magnetic assisted cell
sorting.
A key aspect of the Streptamer approach is the ability to rapidly remove the staining reagent after the
isolation step by disassembly of the MHC/Strep-Tactin multimer in the presence of low concentrations
of d-biotin at 4 C, which competes with high affinity for the binding of Strep-tag to Strep-Tactin. The
weak binding monovalent MHCs dissociate spontaneously and rapidly from the T-cell surface. The
realization of a truly reversible staining enables for the first time the collection of fully functional
T-cells that are indistinguishable from untreated cells. This is in contrast to the unfavorable effects of
conventional MHC multimer staining techniques, especially regarding the consequences for the
outcome of adoptive T-cell transfers.
Strep-Tactin
Fluorescently Labeled Strep-Tactin
Part # Status Fluorochrome Size
S00010 PE 50 tests RUO
S00020 APC 50 tests RUO
377
Streptamer
Streptamer Magnetic Beads provide a rapid and economic alternative for T-cell isolation at 4C under
sterile conditions by Magnet Assisted Cell Sorting. We are pleased to announce the new Streptamer
Magnetic Beads for T-cell isolation by magnetic cell separation. Due to our unique Streptamer
technology positively selected antigen-specific T-cells are phenotypically and functionally
indistinguishable from untreated cells. In addition to IBA's fluorescent conjugates allowing for
sensitive T-cell detection and T-cell isolation, the paramagnetic beads enable a rapid and economic T-
cell isolation at 4C under sterile conditions.
Easy-to-handle T-cell purification with magnets
Fast and sterile
Reversible T-cell labelling
Functional isolation and characterization of T-cells
Mild, rapid and complete removal of Streptamer Beads using biotin
Improved viability of T-cells after isolation
Bead isolates can be ideally detected with fluorescent conjugates
Streptamer Magnetic Beads
Magnetic Beads
S00030 - 5 preps RUO
Solution Sets
378
Streptamer
Fluorescently labeled Streptamers can be used for T-cell staining or T-cell isolation using Fluorescence
Assisted Cell Sorting.
Reversible, antigen-specific staining of T-cells.
Isolation and characterization of functional T-cells
High staining intensities
Mild and rapid dissociation of staining reagents using biotin
Improved viability of T-cells after isolation
Analysis with Phycoerythrin (PE) or Allophycocyanin (APC)
Class I Streptamer
Streptamer Tools for T-Cell Research
Cancer
BST-2
HLA-A*0201
KLQDASAEV
S01084 PE 50 tests/25 preps RUO
S01073 APC 50 tests/25 preps RUO
CAP1
HLA-A*0201
YLSGANLNL
Gp 100
HLA-A*0201
IMDQVPFSV
Gp 100
HLA-A*0201
ITDQVPFSV
379
Streptamer
Gp100
HLA-A*0201
YLEPGPVTA
HA-1H
HLA-A*0201
VLHDDLLEA
Her-2/neu
HLA-A*0201
KIFGSLAFL
Her-2/neu
HLA-A*0201
RLLQETELV
I LLO 91-99
H-2 Kd
GYKDGNEYI
MAGE-A1
HLA-A*0101
EADPTGHSY
380
Streptamer
MART 1
HLA-A*0201
ELAGIGILTV
Muc-1
HLA-A*0201
STAPPVHNV
Muc-1
HLA-B*0702
VPGWGIALL
NY-ESO-1
HLA-A*0201
SLLMWITQV
OVA
H-2 Kb
SIINFEKL
S03000 PE 50 tests/12.5 preps RUO
S03002 APC 50 tests/12.5 preps RUO
Proteinase 3
HLA-A*0201
VLQELNVTV
381
Streptamer
RHAMM
HLA-A*0201
ILSLELMKL
Survivin
HLA-A*0201
ELTLGEFLKL
Tyrosinase
HLA-A*0201
YMDGTMSQV
Uty
H-2 Db
WMHHNMLDI
WT-1
HLA-A*0201
RMFPNAPYL
WT-1
HLA-A*2402
CMTWNQMNL
382
Streptamer
Infectious Disease
CMV pp50
HLA-A*0101
VTEHDTLLY
CMV PP65
HLA-A*0201
NLVPMVATV
CMV pp65
HLA-A*2402
QYDPVAALF
CMV pp65
HLA-B*0702
TPRVTGGGAM
EBV
HLA-A*0201
GLCTLVAML
HBVcore
HLA-A*0201
FLPSDFFPSV
383
Streptamer
HIV gag
H-2 Kd
AMQMLKETI
HIVgag
HLA-A*0201
SLYNTVATL
HIVpol
HLA-A*0201
ILKEPVHGV
Influenza M
HLA-A*0201
GILGFVFTL
384
Streptamer
Viral antigens
ADV hexon
HLA-A*2402
TYFSLNNKF
IE-1
HLA-A*0201
VLEETSVML
IE-1
HLA-B*0801
QIKVRVDMV
385
NOTES
386
Isotypic Controls -------------------------------- 388-395
Secondary Antibodies --------------------------- 396-422
Ancillary Reagents
- High Affinity Molecules ------------------ 423-424
- Detection Kits ---------------------------- 425-426
- Miscellaneous Reagents ----------------- 427-431
387
Isotypic Controls
Canine
IgG (canine)
Part # Size
Form
Format Line Status
731691 Cell Lab Liquid 5 mg UNLB LUO
Chicken
IgY (chicken)
Part # Size
Form
Format Line Status
Goat
IgG (goat)
Part # Size
Form
Format Line Status
731637 Cell Lab Liquid 0.5 mg BIOT LUO
731636 Cell Lab Liquid 100 tests FITC LUO
731638 Cell Lab Liquid 100 tests PE LUO
737067 Cell Lab Liquid 100 tests APC LUO
F(ab')
2
Part # Size Form Format Line Status
Fab
Fc
388
Isotypic Controls
Hamster
IgG (hamster)
Part # Size
Form
Format Line Status
731684 Cell Lab Liquid 0.5 mg UNLB LUO
731685 Cell Lab Liquid 0.5 mg FITC LUO
731687 Cell Lab Liquid 0.1 mg PE LUO
731688 Cell Lab Liquid 0.1 mg APC LUO
721540 Cell Lab Liquid 0.1 mg CY5 LUO
Human
IgA Kappa (human)
Part # Size
Form
Format Line Status
731701 Cell Lab Liquid 0.5 mg UNLB RUO
736749 Cell Lab Liquid 1 mL HRP RUO
736730 Cell Lab Liquid 1 mL AP RUO
IgA Lambda (human)
Part # Size
Form
Format Line Status
IgG (human)
Part # Size
Form
Format Line Status
Fc
IgG1 kappa (human)
Part # Size
Form
Format Line Status
A55700 Cell Lab Liquid 0.1 mg PE RUO
389
Isotypic Controls
IgG1 Lambda (human)
Part # Size
Form
Format Line Status
Monkey
IgG (Rhesus Monkey)
Part # Size
Form
Format Line Status
Mouse
IgA (mouse)
Part # Size
Form
Format Line Status
IgE (mouse)
Part # Size
Form
Format Line Status
IgG (mouse)
Part # Size
Form
Format Line Status
6602398 COULTER CLONE Freeze- 100 tests Purified RUO
731622 Cell Lab Liquid 1 mL AP LUO
6602431 COULTER CLONE Freeze- 100 tests FITC RUO
6603482 COULTER CLONE Liquid 100 tests RD1 RUO
390
Isotypic Controls
IgG1 (mouse)
Part # Size
Form
Format Line Status
6602872 COULTER CLONE Freeze- 100 tests Purified IVD
IM0571 - Liquid 100 tests Purified RUO
IM0639U IOTest Liquid 2 mL FITC ASR
A07795 IOTest Liquid 100 tests FITC CE
6603864 CYTO-STAT Liquid 50 tests FITC IVD & CE
6602928 COULTER CLONE Freeze- 100 tests FITC IVD & CE
IM0670U IOTest Liquid 2 mL PE ASR
A07796 IOTest Liquid 100 tests PE CE
6602884 COULTER CLONE Liquid 100 tests RD1 IVD & CE
IM2714U IOTest Liquid 1 mL ECD ASR
A07797 IOTest Liquid 100 tests ECD CE
6604548 CYTO-STAT Liquid 50 tests ECD RUO
IM2663U IOTest Liquid 1 mL PC5 ASR
A07798 IOTest Liquid 100 tests PC5 CE
6607012 CYTO-STAT Liquid 50 tests PC5 RUO
A62833 IOTest Liquid 100 tests PC5.5 CE
A62834 IOTest Liquid 1 mL PC5.5 ASR
6607099 IOTest Liquid 100 tests PC7 RUO
737662 IOTest Liquid 100 tests PC7 CE
IM2475 IOTest Liquid 100 tests APC CE
IM2475U IOTest Liquid 1 mL APC ASR
731627 Cell Lab Liquid 100 tests CY5 LUO
A71118 IOTest Liquid 1 mL APC-Alexa Fluor* 700 ASR
A79391 IOTest Liquid 100 tests APC-Alexa Fluor* 700 CE
A71120 IOTest Liquid 1 mL APC-Alexa Fluor* 750 ASR
A79393 IOTest Liquid 100 tests APC-Alexa Fluor* 750 CE
A74766 IOTest Liquid 1 mL Pacific Blue* ASR
A74764 IOTest Liquid 100 tests Pacific Blue* CE
A96415 IOTest Liquid 1 mL Krome Orange ASR
F(ab')
2
391
Isotypic Controls
IgG2a (mouse)
Part # Size
Form
Format Line Status
A12690 IOTest Liquid 2 mL FITC ASR
A12689 IOTest Liquid 100 tests FITC CE
A09141 IOTest Liquid 2 mL PE ASR
A09142 IOTest Liquid 100 tests PE CE
6604119 CYTO-STAT Liquid 50 tests RD1 IVD & CE
A09144 IOTest Liquid 1 mL ECD ASR
A09147 IOTest Liquid 1 mL PC5 ASR
A09148 IOTest Liquid 100 tests PC5 CE
A12692 IOTest Liquid 1 mL PC7 ASR
A12694 IOTest Liquid 1 mL APC ASR
A12693 IOTest Liquid 100 tests APC CE
731662 Cell Lab Liquid 100 tests CY5 LUO
IgG2b (mouse)
Part # Size
Form
Format Line Status
6603852 CYTO-STAT Liquid 0.5 mL RD1 ASR
6604700 CYTO-STAT Liquid 0.5 mL ECD ASR
731694 Cell Lab Liquid 0.5 mg BIMA LUO
392
Isotypic Controls
IgG3 (mouse)
Part # Size
Form
Format Line Status
IgM (mouse)
Part # Size
Form
Format Line Status
IM1268 - Liquid 100 tests Purified RUO
IM1269U IOTest Liquid 2 mL FITC ASR
6604117 CYTO-STAT Liquid 50 tests RD1 IVD & CE
Rabbit
IgG (rabbit)
Part # Size
Form
Format Line Status
A88903 Cell Lab Liquid 10 g Alexa Fluor* 488 RUO
F(ab')
2
IgM (rabbit)
Part # Size
Form
Format Line Status
393
Isotypic Controls
Rat
IgG (rat)
Part # Size
Form
Format Line Status
IgG1 (rat)
Part # Size
Form
Format Line Status
IgG2a (rat)
Part # Size
Form
Format Line Status
IgG2b (rat)
Part # Size
Form
Format Line Status
394
Isotypic Controls
IgM (rat)
Part # Size
Form
Format Line Status
721539 Cell Lab Liquid 0.5 mg FLMA LUO
Sheep
IgG (sheep)
Part # Size
Form
Format Line Status
A55699 Cell Lab Liquid 5 mg UNLB LUO
395
To find the secondary antibody you need:
1. Select the species with which you would like the product to react. These species are in alphabetic order and shaded in grey. For
example, if your first antibody is a mouse immunoglobulin (Ig), go to the MOUSE sub section; if it is a rabbit immunoglobulin go to the
RABBIT sub section.
2. Select the host species in which you want the antibody to be made. They are in the following alphabetic order: donkey, goat,
rabbit, sheep, and swine. Not all five species are available for each product.
3. Select the type of Ig you would like to react with. They are in alphabetic order. Within the parenthesis, the fragment specificities are
indicated. For example IgG (Fc) means that the secondary antibody is specific for the Fc fragment. If no fragment specificity is
indicated, that means that the secondary antibody is specific for the heavy chain of the Ig.
4. Select the adsorption you need to minimize cross-reactions. If you work with human cells, select preferably a product that is human
Ig adsorbed. If you work with mouse cells, select preferably a product that is mouse Ig adsorbed.
5. Select, if available, between the whole Ig or only the F(ab')
2
fragment.
6. Select the form.
Secondary Antibodies
Sheep Anti-FITC
Part #
Size Form Format Line Status
732962 Cell Lab Liquid 1 mg BIOT LUO
732961 Cell Lab Liquid 1 mL HRP LUO
Bovine
Goat Anti-Bovine IgG (H+L)
Part #
732834 Cell Lab Liquid 1 mg FITC LUO
Canine
Goat Anti-Canine IgG (H+L)
Part #
396
Chicken
Goat Anti-Chicken IgG (H+L)
Part #
A55752 Cell Lab Liquid 1 mg BIOT LUO
A55753 Cell Lab Liquid 0.5 mg PE LUO
Equine
Goat Anti-Equine IgG (H+L)
Part #
Feline
Goat Anti-Feline IgG (H+L)
Part #
397
Goat
Donkey Anti-Goat IgG (H+L)
Cross adsorbed against rat and mouse Ig
Rabbit Anti-Goat IgG (H+L)
Part #
735167 Cell Lab Liquid 1 mg TRITC LUO
Cross adsorbed against human Ig
Rabbit F(ab)
2
Anti-Goat IgG (H+L)
Part #
398
Swine Anti-Goat IgG (H+L)
Cross adsorbed against human, rat and mouse Ig
Guinea Pig
Goat Anti-Guinea Pig IgG (H+L)
Part #
Hamster
Goat Anti-Hamster IgG (H+L)
Part #
Cross adsorbed against rat and mouse Ig
399
Goat F(ab)2 Anti-Hamster IgG (H+L)
Cross adsorbed against mouse and rat Ig
Rabbit Anti-Hamster IgG (H+L)
Cross adsorbed against mouse and rat Ig
Cross adsorbed against mouse Ig
Human
Goat Anti-Human IgA
Part #
732601 Cell Lab Liquid 1 mg UNLB RUO
732607 Cell Lab Liquid 1 mg BIOT RUO
732602 Cell Lab Liquid 1 mg FITC RUO
732608 Cell Lab Liquid 0.5 mg PE RUO
732603 Cell Lab Liquid 1 mg TRITC RUO
732606 Cell Lab Liquid 1 mg TXRD RUO
400
Goat Anti-Human IgD
Part #
Goat Anti-Human IgG
Part #
732582 Cell Lab Liquid 1 mL BGAL RUO
Goat Anti-Human IgM
Part #
401
Goat Anti-Human IgM + IgG + IgA (H+L)
Part #
Goat Anti-Human Kappa
Part #
A55741 Cell Lab Liquid 1 mg UNLB RUO
A55742 Cell Lab Liquid 1 mg BIOT RUO
Goat Anti-Human Lambda
Part #
402
Goat F(ab')
2
Anti-Human IgA
Part #
732611 Cell Lab Liquid 0.5 mg BIOT RUO
732610 Cell Lab Liquid 0.5 mg FITC RUO
Goat F(ab)
2
Anti-Human IgD
Part #
Goat F(ab')
2
Anti-Human IgG (Fc)
Cross adsorbed against bovine, human and mouse Ig
IM1627 - Freeze-dried 1 mg FITC RUO
IM0550 - Freeze-dried 1 mL PE RUO
Goat F(ab')
2
Anti-Human IgG
Part #
403
Goat F(ab')
2
Anti-Human IgG (H+L)
Cross adsorbed against bovine, human and mouse Ig
IM0837 - Freeze-dried 0.5 mL HRP RUO
Goat F(ab)
2
Anti-Human IgM
Part #
Goat F(ab)
2
Anti-Human IgM + IgG + IgA (H+L)
Part #
Goat F(ab)
2
Anti-Human Kappa
Part #
404
Goat F(ab)
2
Anti-Human Lambda
Part #
Goat F(ab) Anti-Human IgG
Part #
Rabbit Anti-Human IgG (H+L)
Part #
A55754 Cell Lab Liquid 0.5 mg PE RUO
405
Rabbit F(ab)
2
Part #
Mouse
Donkey Anti-Mouse IgG (H+L)
Part #
Goat Anti-Mouse IgA
Part #
732300 Cell Lab Liquid 0.5 mg TRITC LUO
736944 Cell Lab Liquid 0.5 mg TXRD LUO
406
Goat Anti-Mouse IgE
Part #
Goat Anti-Mouse IgG
731837 Cell Lab Liquid 1 mL BGAL LUO
731838 Cell Lab Liquid 1 mg TXRD LUO
Goat Anti-Mouse IgG (H+L)
731851 Cell Lab Liquid 1 mg CY5 LUO
Cross adsorbed against human, bovine and horse Ig
IM0815 - Liquid 1.5 mg Purified RUO
407
Cross adsorbed against rat Ig
Goat Anti-Mouse IgG1
A55702 Cell Lab Liquid 0.5 mg APC LUO
408
Goat Anti-Mouse IgG2a
Goat Anti-Mouse IgG2b
Goat Anti-Mouse IgG2c
409
Goat Anti-Mouse IgM
Part #
410
Goat Anti-Mouse IgM + IgG + IgA (H+L)
Goat Anti-Mouse Kappa
Part #
Goat Anti-Mouse Lambda
Part #
411
Goat F(ab')
2
Anti-Mouse IgG (Fc)
Goat F(ab)
2
Anti-Mouse IgG (H+L)
IM1618 - Liquid 1 mg Purified RUO
IM0816 - Freeze-dried 0.5 mL BIOT RUO
Goat F(ab)
2
Anti-Mouse IgG1
412
Goat F(ab)
2
Anti-Mouse IgG2a
Goat F(ab)
2
Anti-Mouse IgG2b
Goat F(ab)
2
Anti-Mouse IgG3
Goat F(ab)
2
Anti-Mouse IgM
Goat F(ab')
2
Anti-Mouse IgM + IgG (H+L)
413
Goat F(ab)
2
Anti-Mouse IgM + IgG + IgA (H+L)
Goat F(ab)2 Anti-Mouse Kappa
Part #
A55701 Cell Lab Liquid 0.5 mg UNLB LUO
Rabbit Anti-Mouse IgG (H+L)
Part #
414
Rabbit F(ab)
2
Part #
Porcine
Goat Anti-Porcine IgG (H+L)
Part #
Rabbit
Donkey Anti-Rabbit IgG (H+L)
Cross adsorbed against human, rat, mouse Ig
415
Goat Anti-Rabbit IgG
Part #
Goat Anti-Rabbit IgG (H+L)
Cross adsorbed against human and mouse Ig
Goat Anti-Rabbit IgM
Part #
416
Goat Anti-Rabbit IgM + IgG (H+L)
Goat F(ab)
2
Anti-Rabbit IgG (H+L)
IM0830 - Freeze-dried 0.5 mL BIOT RUO
417
Rat
Donkey Anti-Rat IgG (H+L)
Goat Anti-Rat IgG
Part #
Goat Anti-Rat IgG (Fc)
418
Goat Anti-Rat IgG (H+L)
Goat Anti-Rat IgM
Part #
419
Goat Anti-Rat IgM + IgG (H+L)
Goat F(ab)
2
Anti-Rat IgG (H+L)
Goat F(ab) Anti-Rat IgM + IgG (H+L)
420
Rabbit Anti-Rat IgG (H+L)
Part #
Rabbit F(ab)
2
Anti-Rat IgG (H+L)
Part #
Rhesus
Goat Anti-Rhesus IgG (H+L)
Part #
421
Sheep
Rabbit Anti-Sheep IgG (H+L)
Part #
Rabbit F(ab)
2
Anti-Sheep IgG (H+L)
Part #
Turkey
Goat Anti-Turkey IgG (H+L)
Part #
422
Streptavidin and avidin products are useful secondary reagents, as they bind to biotin attached to a
primary antibody or protein. A wide range of streptavidin and avidin reagents is offered, and a
variety of formats are available to allow the uniform matching of conjugate/fluorochrome types
within an experiment.
Antibody binding proteins are useful in many basic techniques such as affinity chromatography,
immunoprecipitation, ELISA, immunoblotting and immunohistochemistry. Three of such proteins are
offered - proteins A, G and L.
Protein A binds the Fc portion of human IgG1, IgG2, IgG3 (weakly), IgG4, and mouse IgG1
(weakly), IgG2a, IgG2b, and IgG3. Protein A also binds IgG from monkey, rabbit, pig, Guinea pig,
dog, and cat. Protein G binds the Fc portion of IgGs from many species including all human, and
mouse (IgG1, weakly) subclasses. It does not bind IgM, IgA, IgE or serum albumin. Protein L binds
Ig, primarily through kappa () light chains, from a variety of sources. Protein L does not bind to
bovine, sheep, goat or chicken Igs.
High Affinity Molecules
Avidin
Format or Conjugate Status
Part #
Size Format
UNLB 733006 5 mg LUO Liquid
HRP 733010 1 mL LUO Liquid
AP 733009 1 mL LUO Liquid
BGAL 733011 1 mL LUO Liquid
FITC 733007 1 mg LUO Liquid
PE 733013 0.5 mg LUO Liquid
APC 733014 0.1 mg LUO Liquid
CY5 735907 1 mg LUO Liquid
TRITC 733008 1 mg LUO Liquid
TXRD 733012 1 mg LUO Liquid
Protein A
Part #
Size Format
UNLB 733017 1 mg LUO Lyophilized
Protein G
Part #
Size Format
Protein L
Part #
Size Format
423
High Affinity Molecules
S-Tag
Part #
Size Format
UNLB A55748 0.5 mg LUO
Clone: SBSTAGa (IgG1 mouse)
Liquid
Clone: SBSTAGb (IgG2a mouse)
Liquid
Clone: SBSTAGc (IgG2b mouse)
Liquid
FITC A55750 0.5 mg LUO
Clone: SBSTAGb (IgG2a mouse)
Liquid
Streptavidin
Part #
Size Format
UNLB 732990 0.5 mg LUO Liquid
UNLB 732989 5 mg LUO Liquid
HRP IM0309 1 mg RUO Freeze-dried
AP 732994 1 mL LUO Liquid
BGAL 732996 1 mL LUO Liquid
FITC IM0307 1 mg RUO Freeze-dried
FITC IM3324 100 tests RUO Liquid
FITC 732992 0.5 mg LUO Liquid
FITC 732991 1 mg LUO Liquid
PE IM0557 1 mL RUO Freeze-dried
PE IM3325 100 tests RUO Liquid
PE 732998 1 mg LUO Liquid
ECD IM3326 100 tests RUO Liquid
PC5 IM3327 100 tests RUO Liquid
PC7 IM3659 100 tests RUO Liquid
APC 733001 1 mg LUO Liquid
CY5 733005 1 mg LUO Liquid
TRITC 732993 1 mg LUO Liquid
TXRD 732997 1 mg LUO Liquid
424
Streptavidin-Biotin Method
Beckman Coulter, Inc. detection kits are intended for the visualization by light microscopy of antigens in
paraffin embedded tissues, frozen sections and cytological preparations using the streptavidin-biotin affinity
method.
The UltraTech Detection Kits are ultra sensitive kits, based on a fast procedure that gives intense staining.
The UltraTech kit can be used with mouse, rat and rabbit primary antibodies. For optimal use of the UltraTech
system, each primary antibody should be incubated at a dilution validated and documented by the user,
depending on the standard incubation time in use in the laboratory.
- The 70 test kit contains all the necessary reagents for the completion of the procedure.
- The 500-600 test kit contains the immunological reagents, but not the chromogen system which can be
ordered separately.
The Universal Detection Kits utilize the standard procedure. These kits are polyvalent and can be used with
all primary antibodies derived from rabbit, goat, mouse, guinea pig, sheep or rat.
- The 50-100 test kit contains all the essential reagents necessary for the completion of the procedure.
- The 500-700 test kit contains the immunological reagents, but not the chromogen system which can be
ordered separately.
Detection Kits
UltraTech Streptavidin-Biotin System 70 tests
Status Part #
Status Part #
UltraTech-HRP-AEC
Volume Component
IM2764 RUO
H
2
O
2
1 x 8 mL
Blocking Agent 1 x 8 mL
Biotinylated secondary antibody 1 x 8 mL
Streptavidin-Peroxidase 1 x 8 mL
AEC concentrate 1 x 3 mL
Substrate buffer 7 x 5 mL
Status Part #
UltraTech-HRP-DAB
Volume Component
IM2765 RUO
H
2
O
2
1 x 8 mL
DAB concentrate 1 x 3 mL
425
Detection Kits
UltraTech Streptavidin-Biotin System 500-600 tests
Status Part #
Status Part #
UltraTech-HRP
Volume Component
IM2391 RUO
Status Part #
UltraTech-AEC
Volume Component
IM2393 RUO
AEC concentrate 1 x 3 mL
Status Part #
UltraTech-DAB
Volume Component
IM2394 RUO
DAB concentrate 1 x 3 mL
426
Ancillary Reagents
Apoptosis Support Reagents
Liquid, 10X 731728 50 mL RUO
ApoScreen* Annexin V Binding Buffer
Annexin V Binding Buffer, 10X concentrate, is formulated for use with all ApoScreen* reagents.
Format or Conjugate Status Part # Size
1 vial 731727 100 tests RUO
ApoScreen* Propidium Iodide
Buffers
Powder 6603369 15 packets LUO
PBS Buffer
This package contains 15 foil-wrapped packets of PBS Buffer reagent. Each package is brought up to a
500 mL volume with distilled water to yield a 0.01 M potassium phosphate, 0.15 M sodium chloride at a
pH of 7.2 0.2.
EMC Proteins
Lyophilized 731955 1 mg LUO
Goat Fibronectin
Source: Fresh goat plasma.
Purification: Affinity chromatography on gelatin covalently linked to agarose.
Purity: >95% as judged by SDS-PAGE analysis (Mr 50 kDa)
Liquid 731954 1 mg LUO
Mouse Laminin
Source: EHS sarcoma.
Purification: The extract is purified by salt precipitation, ion exchange and gel filtration chromatography.
Purity: >95% as judged by SDS-PAGE analysis.
427
Ancillary Reagents
Enzyme Substrates
Powder 731707 500 mg LUO
ABTS substrate
ABTS (2,2-azino-bis-[3-ethylbenzthiazoline-6-sulfonic acid]) is a soluble, safe, convenient substrate for
ELISA applications employing horseradish peroxidase-labeled reagents. It develops an intense blue-
green color measurable between 405 nm and 410 nm. It may be used for both kinetic and endpoint
reactions. ABTS is supplied in both powder form and as a ready to use single component system.
BCIP substrate
BCIP (5-bromo-4-chloro-3-indolyl phosphate) is supplied in powder form. It can be used in combination
with nitroblue tetrazolium (NBT) for preparation of an immunoblotting substrate solution for use with
alkaline phosphatase-labeled reagents.
Liquid 731711 100 mL LUO
BCIP/NBT substrate solution
BCIP/NBTPLUS (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium) is a ready to use single
component system for use in both immunoblotting and immunohistochemical procedures with alkaline
phosphatase-labeled reagents. The system is based upon the hydrolysis of BCIP and reduction of NBT,
producing a deep purple reaction product. The solution is stable at room temperature.
NBT
NBT (nitroblue tetrazolium) is supplied in crystal form. It can be used in combination with BCIP (5-
bromo-4-chloro-3-indolyl phosphate) for preparation of an immunoblotting substrate solution for use
with alkaline phosphatase-labeled reagents.
ONGP
ONGP (o-nitrophenyl--D-galactopyranoside) is an excellent substrate for -galactosidase-based ELISA.
It produces a soluble end-product which is yellow in color and read at 405 - 410 nm. Both kinetic and
endpoint determinations can be performed. ONGP is supplied in powder form.
428
Ancillary Reagents
Tablet 731706 50 x 5 mg LUO
p-NPP
p-NPP (p-nitrophenyl phosphate) is an excellent substrate for phosphatase-based ELISA. It produces a
soluble end-product which is yellow in color and read at 405 - 410 nm. Both kinetic and endpoint
determinations can be performed. It is supplied in tablet form.
TMB substrate solution
3,3,5,5-tetramethylbenzidine (TMB) is a peroxidase substrate which produces a stable, insoluble, dark
blue precipitate at the reaction site with little or no background. It is ideal for many immunoblotting
procedures and can significantly increase the detection limits of assays on a variety of membranes. TMB
is supplied as a ready to use single component system.
Genetically Distinct Collagens
Lyophilized 732453 0.25 mg LUO
Bovine Type I Collagen
Bovine Type II Collagen
Liquid 732460 0.5 mg LUO
Bovine Type III Collagen
Bovine Type IV Collagen
Bovine Type V Collagen
429
Ancillary Reagents
Bovine Type VI Collagen
Liquid 732452 0.5 mg RUO
Human Type I Collagen
Human Type III Collagen
Human Type IV Collagen
Lyophilized 732465 0.1 mg RUO
Human Type V Collagen
Human Type VI Collagen
Mouse Type IV Collagen
Rat Type I Collagen
430
Ancillary Reagents
Immunohistochemistry Products
Fluoromount-G*, slide mounting medium
Fluoromount-G* is an excellent mounting medium for slides mounted after a staining procedure with an
aqueous final step. It is a water soluble, non-fluorescing compound which provides a semi-permanent
seal for storage of slides at 4C.
Liquid IM1480 1 x 15 mL RUO
Resin-based Mounting Medium
not applicable
IM3580 1 unit RUO
Super PAP Pen
Super PAP Pen provides a water-repellent border surrounding each tissue section. This allows the use of
a minimum amount of antiserum or antibody solution.
Normal Sera
Goat Serum
Mouse Serum
Rabbit Serum
Saturation Reagent
Liquid 732802 50 tests LUO
Fc Receptor Saturation Reagent
431
NOTES
432
Clone --------------------------------------------- 434-441
Part Numbers ------------------------------------ 442-457
Alphabetical -------------------------------------- 458-473
433
Clone Name Technical Data Clone Name Technical Data Target Target
Clone
APPROPRIATE USES APPROPRIATE USES INAPPROPRIATE USES
A Activating F Frozen Tissue Sections A Non-activating
B Blocking I Immunoprecipitation B Non-blocking
C Flow Cytometry P Paraffin-embedded Tissue Sections I Inappropriate for Immunoprecipitation
Ci Intracytoplasmic Staining Ph Heat Treated Paraffin-embedded Tissue W Inappropriate for Western Blot
E Elisa W Western Blot
Most of the Beckman Coulter Monoclonal Antibodies to human CD antigens have been clustered and studied during at least one of the
International Workshops on Human Leucocyte Differentiation Antigens (HLDA). The blue number references the HLDA workshop at which an
antibody was clustered.
1 HLDA 1: Leucocyte Typing - Bernard, A., et al. Eds., Springer-Verlag (1984)
2 HLDA 2 : Leucocyte Typing II - Reinherz, E.L., et al. Eds., Springer-Verlag (1986)
3 HLDA 3: Leucocyte Typing III - McMichael, A.J., et al. Eds., Oxford University Press (1987)
4 HLDA 4: Leucocyte Typing IV - KnappW., et al. Eds., Oxford University Press (1989)
5 HLDA 5 Leucocyte Typing V - Schlossman, S.F., et al. Eds., Oxford University Press (1995)
6 HLDA 6 Leucocyte Typing VI - Kishimoto, T., et al. Eds., Garland Publishing, Inc. (1997)
7 HLDA7 Leucocyte Typing VII - Mason, D., et al. Eds., Oxford University Press (2002)
8 HLDA 8 Leucocyte Typing VIII - in Press (2004)
The Technical Data are published information about a clone functional or technical applications. They are not systematically tested in our
laboratories and for each application, you must select the appropriate format of the product. Please refer to the related package insert for
specific information and/or references on intended use or limitations on the use of the products. Each abbreviation indicate technical data that
may be appropriate or inappropriate uses of a product (see table below).
1-2
1A4CD27 124 5 C F I B Human
1A6 229 P P Human
1B4B1 287 E C Mouse
1B8 278 C I Mouse
1C10 262 C I A Mouse
1D3 115 5 C I F Human
1D4B 276 C I W F Mouse
1F11 126 5 C I Human
1G10 271 I B C Mouse
1G11 160 5 C I B F Human
1G2 159 5 C W I F Human
1HT44H3 123 2 I C Human
2-191 306 C F I Chicken
2.7A6A3 195 C W E Ci Human
2A 8F4 302 C Rat
2A3 199 Human
2A9 320 C Rabbit
2B 10A8 302 C Rat
2B8 277 C I Mouse
2C 8F1 302 C Rat
2C4 320 C F I Rabbit
2C75 310 C Chicken
2D10 272 C I F Mouse
2D11 223 C Human
2D12 229 W E Human
2D2 196 Human
2D6 257 C B F P Mouse
2E1 127 3 C I F E N Human
2E7 274 C I F A Mouse
2F1 291 C I Mouse
2F11 200 Human
2F12 204 Human
2F7 271 C F I B Mouse
2F9 349 C Human
2G11 313 C Chicken
2G5 49, 159 5 C I Human
2G8 257 C I Mouse
2G9A10F5 242 5 C Human
2H12 197 Human
2H2D9 240 C W I F Human
2H4LDH11LDB9 44, 45, 140 3 C F I B Human
2RB 165 5 C B E I Human
2ST8.5H7 107 5 C I Human
3-4
3-298 306 C F I W Chicken
3-4F4 316 C F I Feline
3.29B1.10 182 B C F Human
3A1E-12H7 43, 105 1 C F I Human
3A33 254 C F I Mouse
3A6 179 6 C Human
3B5E7.2 198 P F I Human
3C4 274 C I F B Mouse
3C7 259 C B Mouse
3D11 233 C Human
3E11 330 C Human
434
Clone
3E2-25-5 178 6 C W P F Human
3G8 46, 47, 48, 51,
115
4 C F B I A E Human
4A5 179 E C B Human
4B4LDC9LDH8 44, 125 3 C A B I F P Human
4D9 216 C Ci Human
4E3 212 C Human
4E4 228 C E Human
4EL-1C7 123 2 C I B Human
4F6 199 Human
4F9 244 C Human
4G7 180 7 C Human
4H8E6 252 C I F W A Mouse
5-6
5-11G2 310 C F I Chicken
5.6E 126 4 C W I F Human
5A10 229 F P W Human
5B12 194 Human
5B7 279 W Mouse
5D4 201 P F P E W Human
5D6 207 Human
5D8-8-12 178 6 B C W P F Human
5F7 198 W Human
5G10 204 Human
5G3 325 Human
5H10-27 267 B C F Mouse
5H10.21.5 178 C E I W Human
5H2 340 C Human
5H4 277 C I Mouse
6A7 196 Human
6D5 256 C I Mouse
6E1 221 E Human
6E2 333 C Human
6F10.3 100 6 C A Human
7-8-9
7.1 194 I C F W Human
7B2.5 197 C Human
7C11 158 5 C F A Human
7D4 259 C I W F Mouse
7E4 116 6 B I C Human
7G7 163 8 C B Human
7H5 154 7 C Human
8A3 160 C Human
8H8.1 48, 50, 105 2 C F I Human
9-49 209 C Human
9B11 335 C Human
9G7 354 C Human
9H10 278 C I B Mouse
9P25 48, 50, 121 6 C B I Human
10-50
10-1.D2 270 C B Mouse
10C2 279 W Mouse
11-26c 285 I C E Mouse
11-44-2 285 E C Mouse
11.1CN5 227 C Human
11B2G3 201 C Human
11C1.3 204 E F C Human
11E4B-7-6 46, 47, 49, 185 7 C F Human
12-15 249 C I B Mouse
12G5 181 7 C B Human
13B8.2 45, 46, 47, 48,
49, 51, 102
3 W E C B Human
14-4-4S 284 C I F Mouse
14B11 295 C Mouse
15-5-5S 282 C Mouse
16-3-22S 283 Mouse
16G8 348 C Human
17-3-3S 284 C Mouse
18D3 273 C I Mouse
20d5 298 C B Mouse
20E3 343 C Human
21-1A4 310 C F I Chicken
22 47, 149 4 C I F Human
23G3 286 F E C Mouse
24E10 332 C Human
25-9-3 283 C Mouse
25.3 109 4 I C B Human
27-11-13S 281 C F Mouse
27B7 345 C Human
28-14-8 281 C F B Mouse
28B10 347 C Human
30-F1 258 C I Mouse
30-H12 273 C F Mouse
31-7-4 212 C Human
435
Clone
34-1-2S 282 C F Mouse
34-5-3 283 Mouse
34-5-8S 282 C Mouse
36-7-5 283 C F Mouse
37.51 260 C I Mouse
38.13 151 4 C F A Human
39C1.5 45, 100 2 A B C Human
42D1 155 C Human
45.15 209 C Ci Human
51-99
53-6.7 253 C F I Mouse
54H6 327 C Human
56C5.2 234 C F Human
58D6 351 C Human
62.463.2D5.2 186 F P W Human
67A4 192 W I F Human
69 134 5 C E Human
71G4B7 355 C Human
74-12-4 318 1 C I B Pig
74-22-15 319 1 C F I Pig
76-2-11 318 1 C I F p Pig
76-7-4 317 1 C F I Pig
76-E6 279 E W Mouse
80H3 150 5 C I W F E Human
80H5 48, 114 5 C E A P F Human
80M2 164 C Human
83-8B 196 Human
84H10 144 3 I C F B E Ci Human
87G3 341 Human
88H7 150 6 C W F Human
89B 44, 117 2 C I F Human
90 261 C F I Mouse
91 258 C F I Mouse
93 255 C F P B Mouse
93H1 346 C Human
94 110 1 C W I B F Human
95C3 162 5 C I F Human
97A6 182 7 I C Human
100 &
104 264 C I F Mouse
104.G4 183 7 F I Ci Human
104D2D1 49, 163 6 C Human
116 44, 113 1 C I F Human
136F12 356 C Human
145-2C11 249 C I F A Mouse
156-3C11 324 C Human
187.1 292 E C Mouse
191B8 178 6 C B Human
193H12 338 Ci Human
236B4 337 C Human
322A-1 113 1 C F W I E B Human
346-11A 275 F C I W Mouse
36213 232 C Human
366 112 1 C I F Human
390 261 C F I B Mouse
500-A2 250 C Mouse
581 49, 50, 130 5 A C W F P Human
695H1-9-9 299 C Mouse
906 128 2 C F I Human
A
A-1 311 C F E Chicken
A20 264 C I F Mouse
A3 157 5 C I Human
A35 209 E Human
A9604D2 210 C Human
Ab81 328 C Human
ABL-93 276 C F I Mouse
AF23 238 C Human
AICD58 146 5 C I B F Human
ALB1 48, 49, 108 F C Human
ALB11 47, 140 5 C P Human
ALB12 138 C Human
ALB2 109 3 C F Human
ALB6 108 3 C I W A F Human
ALB9 122 6 E C F Human
AMF7 143 5 I C E B F W Human
AV20 309 C F I Chicken
AV6 308 C I Chicken
AV7 307 C I A Chicken
AZND1 184 7 C F Human
B
B-A38 170 C Human
436
Clone
B1.49.9 46, 49, 123 2 I C B A Human
B120 119 1 C I F B Human
B19.1 252 C I F W A Mouse
B1G6 197 C Human
B27 209 C E I Human
B3102E8 211 Human
B3506B4 210 C Human
B8.12.2 46, 47, 208 C Human
B9.11 45, 46, 47, 48,
51, 107
1 C B I Human
B9.12.1 207 C Human
B9E9 49, 118 5 C Human
BA62.6 237 C F Human
BAB281 192 C Human
BB23-8E6 317 2 C A I Pig
BB6-10A10 319 C F Pig
BB6-11C9.6 319 C F I Pig
Bear1 110 6 C I B F Human
Ber-ACT35 169 C Human
BL13 119 3 C F I E Human
BL14 131 3 C I F Human
BL1a 46, 48, 104 3 C I W B Human
BL37.2 231 C Human
BL4 103 3 C B Human
BL6 99 3 C F I Human
BM16 190 C Human
BNI3 172 6 E F I C Ci Human
BTLA7.2 187 8 C Human
BU15 49, 111 3 F C I Human
BVD10-11C10 218 E W Human
BVD10-40F6 218 E W Human
BVD11-37G10 205 E W Human
BVD13-3A5 205 E W Human
BVD2-21C11 206 E W C Human
BVD2-23B6 206 I E W Human
BVD3-1F9 215 E C Human
BVD4-1D11 289 C E W Mouse
BVD6-24G2 289 E C Mouse
BVD8-3G11 215 E W Human
BY55 177 7 C I B Human
C
C1.7 186 5 A C W Human
C11 336 C Human
C15 230 C F Human
C21 234 C Human
C218 145 6 B C Human
C264 313 C Chicken
C3 195 E F P Human
C31E5E 339 C Human
C363.16A 265 C F I P Mouse
C363.29B 250 C I F A Mouse
C3VLA3 142 F C I Human
C4 223 E Human
C455.1F 266 C I Mouse
C71/16 255 C I W Mouse
CAS1.1.3 236 C Human
CB3-1 152 6 A C I W Human
CD28.2 124 5 A C B Human
CF1 165 5 C E B F Human
CH11 158 5 A C F P E Human
CH92 231 C Human
CIa 311 C I B Chicken
CLB-MPO-1 48, 50, 225 C Human
CLB13.17 48, 222 C Human
CLBGran/12 149 4 C W Ci Human
CLBThromb/6 148 4 W I B C F Ci Human
CT-3 305 C F I Chicken
CT-4 305 C F I B Chicken
CT-8 306 C I W Chicken
CT1 311 C I B Chicken
D
D13.14.4E 342 C Human
D3A7 352 C Human
D3HL60.251 45, 48, 49, 128 4 C I Human
D57.2.2E 349 C Human
D7 294 C F W A Mouse
D9E 338 W I F P C Human
DATK32 293 C F I W Mouse
DBB42 140 6 F P W C Human
DCGM4 183 7 F C I Ci Human
DFT1 136 5 C P W A I F Human
DO-1 227 P Human
DREG56 45, 148 5 C W I B E F Human
437
Clone
DX5 266 C Mouse
E
E10 342 C Human
E124.2.8 211 Human
E17.5F3.15.13 237 C F Human
E59.126 190 C W Human
EB6B 174 6 C I W B Human
ELL1.4 237 C Human
EP42 307 C F I Chicken
EP72 307 C F I Chicken
EP96 305 C F I B Chicken
ER1D5 203 P P W F E Human
F
F15-42-1-5 157 6 C W Human
F21-2 313 C F I W Chicken
F21-21 309 C F I W P Chicken
f43 316 C F I Feline
FA6.152 46, 131 5 C I F E W B Human
fCD8 316 C F I Feline
FES172 176 I C Human
FG35.4 280 C I Mouse
FIN9 234 C Human
FMC7 50, 205 W C Human
G
G-1 312 C F E Chicken
G1 1C5 301 C Rat
G1 7E7 301 C Rat
G7 272 C F A Mouse
G9 350 C Human
Gi9 141 5 B F C I W Human
GK1.5 251 C I F Mouse
GL1 272 C F I B Mouse
GL183 175 6 C I W B Human
GL24 266 C I Mouse
GoH3 143 4 F I B A C W Human
H
H1.2F3 269 C F I A Mouse
H106.771 286 E C Mouse
H10E12F4 203 C Human
H132 235 C Human
H139-52.1 292 E C Mouse
H17N 217 C B Human
H2 211 C Human
H28 297 Mouse
H299 44, 118 1 C F I B E Human
H398 164 C Human
H57-597 299 I F P C Mouse
HA5.2B7 156 C F Human
HB15a 154 5 C I A Human
HBF-719 296 C Mouse
HD237 44, 118 3 C B Human
HD239 120 4 N I F C Human
HD50 121 3 C B N I Human
HK1.4 294 C F Mouse
HM47 50, 152 5 I Ci F P C Human
HM79-12 270 C F I W Mouse
HP-3B1 158 5 B I A C F Human
HP-6054 223 Human
HP-F1 155 C Human
HP2/1 142 4 I B C F Human
HP2/19 143 5 C A E I F Human
HP6002 212 C Human
HP6014 213 C Human
HP6023 214 C Human
HP6025 213 C Human
HP6029 211 Human
HP6050 213 C Human
HP6061 211 Human
HRS4 126 3 C W P I E F Human
HT1 + HT4 + HT8 + HT9 240 C Human
I
I21/7 253 C I F W Mouse
I3/2.3 263 C F I Mouse
IADB6 210 C Human
ID1 170 6 C Human
ID2C3 243 P F C Human
IG125 238 C Human
IMMU 157 232 C F Human
IMMU 360 239 C Human
IMMU 389 239 C Human
Immu-357 46, 50, 51, 208 C Human
438
Clone
Immu103.44 112 5 C Human
Immu133 129 5 C W P F A I Human
Immu19.2 47, 51, 138 6 C Human
IMMU510 230 C I E F Human
IP26A 230 C Human
IPM2 243 C Human
ISA-3 188 C Human
J
J.173 136 3 C A I Human
J.33 47, 48, 49, 50,
137
3 C Human
J1B3 202 C Human
J3-119 43, 45, 46, 47,
48, 49, 50, 51,
4 B I C Human
J3D3 130 3 I C E B F P Human
J4.48 141 4 I C W Human
J4.57 141 4 C I P Human
J5 44, 108 1 F C I P B Human
JC5-1 293 E Mouse
JDC-10 212 C Human
JDC-12 223 C Human
JES1-39D10 217 E C W Human
JES1-5A10 217 E Human
JES10-2E10 220 Human
JES10-35G12 220 E Human
JES10-5A2 220 C Ci E W Human
JES3-12G8 219 E W Human
JES3-9D7 219 E W C Ci Human
JES5-16E3 290 E C Mouse
JES5-2A5 290 E W Mouse
JES6-1A12 288 E I W Mouse
JES6-5H4 288 C E I W Mouse
JS11KSC2.3 144 6 I F C Human
JS64 153 5 C I W Human
JU74.3 236 C Human
K
K 4F5 303 C Rat
K20 125 3 I F C B N Human
KBA62 224 P W F P Human
KC56 44, 138 5 C Human
KC57 207 C Human
KDR-1 191 8 C Human
Ki-67 221 F W P C Ci Human
KL1 202 W P F Human
KM201 262 C I W Mouse
KOR-SA3544 222 C W P Human
KP-53 221 Human
KRA236 184 C Human
KUL01 314 C F P Chicken
L
L-1 312 C F Chicken
L161 99 3 C I F Human
L54E2 326 Human
Lia1/2 125 3 I A Human
LO-MG2b-2 286 C Mouse
LO-MG3-7 287 C Mouse
LS198-4-3 46, 132 5 C Human
LT40 308 C I Chicken
M
M 2A1 302 Rat
M-1 312 C F I Chicken
M-4 312 A Chicken
M/K-2 275 C I F B Mouse
M1/70 254 C I F B Mouse
M91 166 6 F I B W C E Human
MA127 226 C Human
MA152 226 C Human
MAB104 153 6 C I F Human
MAB89 133 4 C P A F W E Human
MB19-1 256 C I Mouse
mCD30.1 260 C Mouse
MECA-367 297 C F I W B Mouse
MEL-14 268 C F I B Mouse
MJ7/18 275 C F W Mouse
MP1-22E9 280 E C W Mouse
MP1-31G6 280 E C W Mouse
MP2-43D11 288 E W Mouse
MP2-8F8 288 C E Mouse
MP4-25D2 216 C E W Human
MP5-20F3 290 E C W Mouse
MP5-32C11 290 E W Mouse
MP6-XT22 300 E C W Mouse
MP6-XT3 300 E W Mouse
MPB2D5 231 C Human
MQ2-13A5 218 C E I W Human
439
Clone
MQ2-39C3 218 E W I Human
MR1 278 C I B Mouse
N
N7.48A 215 C Ci Human
N901 43, 44, 46, 48,
49, 145
4 P I F B A C Human
NC1 47, 146 5 C W P Human
NIMR-4 297 C Mouse
NIMR-5 261 C F I Mouse
O
O10 99 4 C I F P Human
ON72 191 C Human
OX-1 301 C Rat
OX-22.2 301 C Rat
P
P-Tyr-100 353 C Human
P10 241 F C Human
P2 49, 134 5 C B E I W Human
P282E 147 5 C Human
PD-L1 188 8 C Human
PD-L2 187 8 C Human
PD1 189 8 C Human
PK136 298 C I A Mouse
Pool 242 Human
PPT3 317 2 C F I A Pig
PR10A9 229 F P Human
PS/2 267 C I F Mouse
PV-1 260 C I A Mouse
PV404.19 173 6 B C I Human
PY20 228 C Human
Q
QBEnd10 129 4 C W P F I A Human
QBEnd10 + Immu133 +
Immu409
130 Human
R
R1.302.12 161 7 C Human
R17217 269 C I B Mouse
R2.477.1 161 7 C Human
R34.34 167 6 B C I Human
R9.12 239 Human
RA3-6B2 265 C F I P Mouse
RB6-8C5 295 C I F W Mouse
RDR34 321 C F I Rabbit
RF3 173 6 C I E Human
RMO52 47, 48, 51, 113 6 C Human
Ros220 + ALB12 138 Human
S
S456C9 166 6 W I B C Human
SA-DA4 214 C Human
SAM1 142 5 C F I B Human
SB12e 224 W E F P Human
SB13a 224 E F P Human
SB14d 224 E F P Human
SB15c 225 E F P Human
SB2H12 194 Human
SB62a 320 Rabbit
SB84a 286 C Mouse
SBGSK32 206 Human
SCO6 162 6 C W I Human
SF1.340 169 6 I C B Human
SFCI12T4D11 43, 44, 45, 103 1 F C B Human
SFCI19Thy1A8 99 1 C I F Human
SFCI21Thy2D3 43, 44, 45, 106 1 C F B I Human
SFCI24T6G12 104 1 C W I F Human
SFCI3Pt2H9 43, 44, 100 1 C B F Human
SJ10.1H11 120 3 C A I Human
SJ1D1 48, 50, 112 3 C I F Human
SPAN 12 241 C Human
SPVL3 207 I N B F Human
SSDCLY107D2 45, 165 C Human
SZ1 135 5 C I W Human
SZ2 135 5 C B I W P Human
SZ21 147 5 C I W E P F Human
SZ22 134 5 C W E I Human
T
T16 47, 49, 132 3 F I C Human
TAMAYA1.2 236 C F Human
TC11-18H10 291 E C W Mouse
TC11-8H4 291 E Mouse
TCR-1 314 C F I B Chicken
TCR-2 314 C F I Chicken
TCR-3 315 C F I Chicken
TEA 1/31 171 6 F I C Human
TEA 1/34 171 6 C F Human
Thy1/310 157 C Human
440
Clone
TP1.55.3 151 C A I F Human
TR5.7.5 168 C Human
TRAP-1 172 6 B C I W F Human
TRFK4 289 E Mouse
TRFK5 289 C E W Mouse
U
UB2 158 F C P Human
UC7-13D5 299 C I F P Mouse
UCHL1 45, 139 3 C P F I B Human
UCHT1 43, 44, 45, 46,
47, 48, 49, 50,
1 I C A F E Human
UHB 214 C Human
UHB 214 Human
UIC2 185 B C F P Human
V
V152 331 C Human
V9 244 F W P E Human
VER2.32.1 235 C Human
W
WEDE15 241 C E Human
WJF24 232 C Human
X
X35 102 3 A E F Human
XMG1.2 284 E C W Mouse
Y
YDJ1.2.2 49, 151 5 C F Human
YLI-90 296 C Mouse
YN1/1.7.4 268 C I F W B Mouse
Z
Z176 228 C W Human
Z199 177 I C B Human
Z231 193 C Human
Z25 193 C Human
Z27.3.7 176 I C B Human
ZB4 159 B C Human
ZIZOU4 233 C Human
ZM3.8 45, 156 I C Human
ZOE 233 C Human
441
Part # Page Part # Page Part # Page Part # Page Part # Page
Part Numbers
A
A07405 77
A07406 184
A07407 184
A07408 155
A07409 181
A07412 77
A07413 190
A07414 159
A07415 79
A07481 171
A07483 171
A07704 36
A07705 89
A07706 89
A07707 84
A07708 86
A07709 88
A07710 84
A07711 86
A07712 88
A07713 86
A07714 87
A07715 85
A07716 88
A07717 87
A07718 87
A07719 89
A07720 88
A07721 85
A07722 85
A07723 87
A07724 89
A07725 84
A07726 84
A07727 86
A07728 85
A07729 90
A07730 90
A07731 90
A07732 90
A07733 78
A07734 78
A07735 78
A07736 78
A07737 79
A07738 81
A07739 82
A07742 99
A07743 100
A07744 100
A07745 100
A07746 101
A07747 101
A07748 101
A07749 101
A07750 102
A07751 102
A07752 102
A07753 104
A07754 104
A07755 105
A07756 107
A07757 107
A07758 107
A07759 108
A07760 108
A07761 108
A07762 112
A07763 112
A07764 113
A07765 113
A07766 115
A07767 115
A07768 117
A07769 117
A07770 117
A07771 117
A07772 118
A07773 118
A07774 123
A07775 128
A07776 130
A07777 130
A07778 132
A07779 132
A07780 132
A07781 134
A07782 137
A07783 137
A07784 137
A07785 137
A07786 140
A07787 139
A07788 145
A07789 145
A07790 148
A07791
119, 205
A07792 185
A07793 208
A07794 82
A07795 391
A07796 391
A07797 391
A07798 391
A07799 41
A07800 41
A07802 40
A07803 40
A08932 104
A08933 160
A08934 191
A09141 392
A09142 392
A09144 392
A09147 392
A09148 392
A09777 43
A09778 174
A10974 82
A11894 42
A11895 42
A12689 392
A12690 392
A12692 392
A12693 392
A12694 392
A12695 83
A17116 50
A17599 94
A21689 100
A21690 104
A21691 130
A21692 145
A21693 118
A22327 201
A22328 190
A22329 191
A22330 228
A22331 113
A22332 174
A22333 175
A22334 155
A22359 164
A22360 164
A22361 179
A22362 168
A22363 170
A22364 171
A23413 77
A23416 77
A24986 169
A24987 227
A25574 26
A25575 26
A25576 26
A25577 26
A25578 26
A25579 26
A31894 217
A32346 163
A32526 196
A32535 165
A32536 119
A32537 136
A32560 136
A32909 248
A32910 248
A33096 104
A33097 112
A33098 115
A33099 121
A35473 23
A36700 301
A38415 18, 58
A39499 230
A39500 230
A40174 211
A40316 170
A40317 170
A40579 208
A40926 226
A44224 247
A44225 247
A44226 154
A44227 184
A46526 105
A46527 108
A46528 112
A46529 156
A49073 246
A49080 188
A51074 100
A51075 104
A51076 118
A51077 130
A51078 145
A51079 148
A52635 226
A52882 123
A53365 165
A54139 137
A54189 132
A54190 170
A54191 170
A54822 110
A54823 124
A54824 128
A55699 395
A55700 389
A55701 414
A55702 408
A55703 408
A55706 409
A55707 409
A55708 206
442
Part Numbers
A55709 207
A55710 204
A55711 194
A55712 194
A55713 228
A55715 229
A55716 224
A55717 224
A55718 224
A55719 225
A55721 199
A55726 199
A55727 200
A55728 204
A55729 223
A55730 286
A55731 286
A55732 286
A55734 258
A55735 269
A55736 264
A55738 258
A55740 277
A55741 402
A55742 402
A55744 320
A55745 320
A55746 320
A55747 320
A55748 424
A55749 424
A55750 424
A55751 424
A55752 397
A55753 397
A55754 405
A55756 313
A55757 313
A55758 313
A55759 310
A55760 310
A55761 310
A55762 310
A55763 286
A60492 301
A60790 104
A60791 120
A60792 132
A60793 152
A60794 100
A60795 176
A60796 176
A60797 177
A62833 391
A62834 391
A62835 137
A63492 35
A63493 34
A63663 239
A64615 191
A64616 191
A64617 167
A64618 167
A64827 223
A64828 221
A66085 160
A66286 55
A66287 55
A66288 55
A66327 101
A66328 117
A66329 101
A66330 115
A66331 118
A66332 107
A66333 163
A66898 174
A66899 174
A66900 175
A66901 175
A66902 192
A66903 193
A66904 193
A66905 234
A66906 182
A66907 230
A69183 34
A69184 35
A69964 121
A70188 59
A70198 128
A70200 128
A70201 105
A70202 105
A70203 104
A70204 113
A70205 132
A70207 121
A71116 167
A71117 137
A71118 391
A71119 137
A71120 391
A71564 185
A74763 137
A74764 391
A74765 137
A74766 391
A74775 114
A74777 118
A74779 146
A74781 208
A74788 120
A78835 104
A78836 104
A78837 117
A78838 117
A78882 187
A78883 187
A78884 188
A78885 189
A78886 203
A79386 123
A79388 145
A79389 112
A79390 137
A79391 391
A79392 137
A79393 391
A80249 111
A80704 13
A80705 13
A80706 13
A80710 151
A80711 151
A80712 120
A82789 102
A82790 104
A82791 107
A82792 115
A82943 145
A82944 123
A82946 140
A82959 28
A82960 28
A82961 28
A83477 118
A83478 117
A83479 103
A83480 106
A83481 101
A83482 113
A84175 28
A86049 132
A86050 140
A86051 163
A86052 113
A86054 15
A86056 15
A86057 15
A86061 15
A86062 15
A86063 15
A86353 108
A86354 130
A86355 117
A86356 123
A87782 110
A87783 112
A87784 152
A87785 122
A87786 131
A87787 170
A87789 153
A87939 126
A88535 264
A88536 264
A88537 264
A88538 264
A88539 264
A88540 264
A88541 264
A88542 264
A88543 264
A88544 255
A88545 255
A88546 249
A88547 252
A88548 252
A88549 253
A88550 254
A88551 254
A88552 254
A88553 294
A88554 298
A88555 256
A88556 256
A88557 298
A88558 298
A88559 256
A88562 256
A88563 277
A88564 277
A88565 275
A88568 262
A88569 265
A88570 261
A88571 266
A88572 268
A88573 269
A88574 271
A88575 257
A88576 257
A88577 258
A88578 258
443
Part Numbers
A88579 258
A88580 259
A88581 259
A88582 259
A88583 273
A88584 273
A88585 263
A88586 263
A88587 263
A88588 263
A88589 263
A88591 265
A88592 265
A88593 265
A88595 250
A88596 250
A88597 250
A88598 250
A88599 250
A88601 251
A88602 251
A88603 251
A88604 251
A88606 253
A88607 253
A88608 253
A88609 253
A88610 253
A88871 354
A88875 335
A88878 354
A88879 335
A88880 338
A88881 338
A88882 333
A88883 356
A88884 327
A88885 355
A88886 340
A88887 337
A88888 326
A88889 355
A88890 325
A88891 339
A88903 393
A88904 393
A88906 332
A88907 328
A88909 393
A88911 324
A88912 334
A88914 338
A88915 338
A88918 331
A88920 331
A88921 342
A88923 345
A88924 352
A88925 352
A88926 345
A88927 393
A88928 342
A88929 342
A88930 336
A88931 336
A88932 347
A88933 347
A88934 349
A88935 334
A88936 349
A88937 330
A88938 349
A88939 346
A88940 346
A88941 351
A88942 341
A88943 348
A88944 350
A88946 353
A88947 353
A88950 329
A88951 344
A88952 344
A88954 343
A88955 343
A89307 136
A89308 149
A89309 130
A89310 108
A89311 120
A89312 115
A89313 151
A89314 185
A93687 101
A93689 121
A94245 137
A94246 137
A94680 101
A94681 117
A94682 102
A94683 107
A94685 102
A94686 107
A96415 391
A96416 137
A96417 102
A96418 117
A97050 105
A97052 110
A98434 149
A98835 170
A99019 106
A99021 230
A99022 132
A99023 106
B
B00067 107
B00068 101
B00069 115
B00070 208
B00845 115
B00846 113
B01175 113
B01176 114
B01177 127
B01680 111
B01681 100
B01682 142
B03279 56
B03284 57
B03285 57
B06025 149
B06026 141
B06204 159
B06376 165
B06498 120
B06499 105
B06666 233
B07084 232
B07225 236
B08164 150
B08466 205
B08467 107
B08488 43
B08757 124
B09684 123
B09685 100
B09979 108
B09980 158
B09981 83
B09982 119
B09983 124
B10244 124
B10245 120
B10246 177
B10247 230
B10738 122
B10821 140
B10822 145
B10824 102
B10825 42
B12696 124
B12699 122
B13035 126
CY
CY20030 14
CY20031 14
CY20033 14
CY20130 14
CY20131 14
CY20133 14
CY30230 59
IM
IM0102 107
IM0107 207
IM0108 208
IM0114 197
IM0115 103
IM0116 104
IM0117 108
IM0118 122
IM0119 123
IM0130 99
IM0145 134
IM0150 244
IM0157 109
IM0165 114
IM0166 150
IM0173 221
IM0174 223
IM0175 151
IM0178 102
IM0180 100
IM0190 110
IM0195 130
IM0258 243
IM0270 297
IM0307 424
IM0309 424
IM0318 159
IM0366 132
IM0395 100
IM0398 102
IM0404 53
IM0409 135
IM0416 207
IM0417 127
IM0442U 100
IM0443U 100
IM0448U 102
IM0449U 102
IM0451U 107
IM0452U 107
IM0457 131
IM0458 131
444
Part Numbers
IM0463U 208
IM0464U 208
IM0466U 146
IM0468U 104
IM0469U 104
IM0471U 109
IM0473U 119
IM0478U 123
IM0479U 123
IM0483 151
IM0483U 151
IM0529 121
IM0529U 121
IM0530 110
IM0530U 110
IM0531U 150
IM0537 140
IM0538 135
IM0539 134
IM0540 147
IM0544 144
IM0550 403
IM0551 412
IM0552 418
IM0555 413
IM0557 424
IM0558 53
IM0571 391
IM0584U 140
IM0585U 105
IM0629 159
IM0639U 391
IM0643 113
IM0645U 113
IM0647 138
IM0648U 135
IM0649U 134
IM0650U 113
IM0670U 391
IM0704 102
IM0705 126
IM0712 111
IM0713 112
IM0717 141
IM0718 134
IM0726U 144
IM0747U 80
IM0764 142
IM0765 131
IM0766U 131
IM0769 143
IM0770 143
IM0771 142
IM0775U 132
IM0778U 112
IM0779U 120
IM0782U 137
IM0786 129
IM0787 129
IM0789 99
IM0791U 125
IM0813 115
IM0814U 115
IM0815 407
IM0816 412
IM0817 412
IM0819 412
IM0825 420
IM0830 417
IM0831 417
IM0833 417
IM0837 404
IM0839 404
IM0845 136
IM0846 141
IM0855 412
IM0860U 109
IM0861 146
IM1063 242
IM1066 139
IM1067 114
IM1068 126
IM1074 244
IM1076 138
IM1077 202
IM1135U 128
IM1143 140
IM1148 234
IM1165U 149
IM1166 146
IM1167 129
IM1179U 128
IM1185 129
IM1189 237
IM1198 81
IM1199U 82
IM1201U 81
IM1203U 82
IM1218U 146
IM1219U 136
IM1226 159
IM1231U 148
IM1233 234
IM1234 237
IM1235 124
IM1236U 124
IM1238U 115
IM1239U 144
IM1244 160
IM1247U 139
IM1250U 129
IM1261 237
IM1268 393
IM1269U 393
IM1280 101
IM1281U 101
IM1282U 101
IM1284U 117
IM1285U 117
IM1287U 78
IM1289U 77
IM1291U 78
IM1293U 78
IM1295U 79
IM1300 82
IM1301 101
IM1304 101
IM1307U 139
IM1310U 78
IM1313 117
IM1315 148
IM1316 221
IM1330 236
IM1342 118
IM1344 203
IM1346U 80
IM1349 230
IM1360U 162
IM1364U
119, 205
IM1366 239
IM1374 133
IM1376 124
IM1379U 80
IM1382 92
IM1383 92
IM1384 93
IM1385 93
IM1387 93
IM1388 93
IM1390 93
IM1400 42
IM1404U 142
IM1407 227
IM1408 229
IM1416U 134
IM1417U 135
IM1418U 230
IM1420 129
IM1423U 114
IM1425 141
IM1427U 112
IM1428U 122
IM1429U 105
IM1430 146
IM1431U 126
IM1433U 109
IM1449 153
IM1451 118
IM1451U 118
IM1455U 118
IM1456 118
IM1459U 130
IM1463 239
IM1464 239
IM1480 431
IM1482 232
IM1483 238
IM1484 238
IM1499 241
IM1502 82
IM1504 158
IM1506 158
IM1510 229
IM1545 203
IM1546 229
IM1551 232
IM1552 232
IM1554 235
IM1558 236
IM1560 236
IM1562 237
IM1567 116
IM1568U 116
IM1570U 116
IM1571U 230
IM1582 234
IM1586 234
IM1587 235
IM1588 230
IM1589 230
IM1590 99
IM1591 198
IM1595 195
IM1597 171
IM1598 125
IM1601 143
IM1604U 149
IM1605 153
IM1607 186
IM1608 186
IM1610 158
IM1612 152
IM1612U 152
IM1614 157
IM1618 412
IM1619 412
445
Part Numbers
IM1620 413
IM1621 413
IM1623 420
IM1626 404
IM1627 403
IM1638U 208
IM1639 208
IM1639U 208
IM1650 91
IM1651 91
IM1654U 150
IM1670 91
IM1671 91
IM1672 92
IM1673 92
IM1739 158
IM1740 201
IM1755U 108
IM1756U 134
IM1757U 135
IM1758 147
IM1758U 147
IM1759U 148
IM1760 111
IM1760U 111
IM1761 239
IM1763 192
IM1767 227
IM1832U 132
IM1833 138
IM1834U 140
IM1835 120
IM1835U 120
IM1836 130
IM1837U 141
IM1838U 207
IM1839U 157
IM1840U 157
IM1842 172
IM1844 145
IM1846 175
IM1853U 153
IM1854U 142
IM1855 143
IM1856U 159
IM1864 185
IM1869 130
IM1870 130
IM1870U 130
IM1871U 130
IM1874 225
IM1874U 225
IM1914U 149
IM1915U 108
IM1916 138
IM1918 202
IM1919 244
IM1920 126
IM1921 114
IM1935 127
IM1936U 133
IM1939 224
IM1942U 99
IM1943U 151
IM1954U 114
IM1976U 153
IM1977 162
IM1978 165
IM1979 166
IM1980U 167
IM2000 142
IM2001U 151
IM2002 233
IM2003 234
IM2004 238
IM2006 231
IM2014 107
IM2018 234
IM2027 230
IM2033U 126
IM2034 124
IM2039U 222
IM2047 236
IM2048 237
IM2049 237
IM2051 238
IM2053U 79
IM2069 154
IM2070 172
IM2071U 124
IM2072 166
IM2073U 145
IM2075U 79
IM2076 78
IM2078U 137
IM2081 231
IM2085 241
IM2088U 195
IM2090 178
IM2091 178
IM2115 133
IM2116 147
IM2119 152
IM2128 202
IM2137 221
IM2138 223
IM2146 229
IM2147 229
IM2207 196
IM2210 185
IM2211U 185
IM2212U 185
IM2213 231
IM2214U 148
IM2216U 172
IM2217U 107
IM2218U 154
IM2219 231
IM2221 152
IM2221U 152
IM2234U 169
IM2272 179
IM2276 158
IM2278U 175
IM2279U 80
IM2280U 81
IM2282 172
IM2283 230
IM2285 232
IM2286 232
IM2287 233
IM2289 234
IM2290 234
IM2291 235
IM2292 235
IM2295 237
IM2353U 115
IM2354 178
IM2355 231
IM2356U 202
IM2357U 222
IM2370U 185
IM2371U 132
IM2372 231
IM2375 52
IM2387 158
IM2388 40
IM2389 40
IM2391 426
IM2393 426
IM2394 426
IM2406 231
IM2407 231
IM2408 233
IM2409 126
IM2410U 154
IM2446U 158
IM2467 101
IM2467U 101
IM2468 102
IM2468U 102
IM2469 107
IM2469U 107
IM2470 117
IM2470U 117
IM2471 128
IM2471U 128
IM2472 130
IM2472U 130
IM2473 137
IM2473U 137
IM2474 145
IM2474U 145
IM2475 391
IM2475U 391
IM2549 240
IM2550 242
IM2557 173
IM2578 124
IM2579 153
IM2580 113
IM2580U 113
IM2581U 110
IM2583 241
IM2584 241
IM2585 51
IM2586 51
IM2634U 100
IM2635U 101
IM2636U 102
IM2637U 104
IM2638U 107
IM2639U 112
IM2640U 113
IM2641U 114
IM2642U 115
IM2643U 117
IM2644U 118
IM2645 122
IM2646 123
IM2646U 123
IM2647 128
IM2647U 128
IM2648U 130
IM2649 129
IM2650U 129
IM2651U 132
IM2652U 138
IM2653U 137
IM2654U 145
IM2655U 148
IM2656 151
IM2656U 151
IM2657U 162
IM2658 186
446
Part Numbers
IM2659U 208
IM2660 195
IM2662 230
IM2662U 230
IM2663U 391
IM2667U 79
IM2673U 81
IM2695U 80
IM2705U 101
IM2707U 113
IM2708U 117
IM2709U 130
IM2710U 137
IM2711U 140
IM2712U 139
IM2713U 148
IM2714U 391
IM2716U 209
IM2717U 209
IM2718U 215
IM2719U 216
IM2720U 108
IM2721U 108
IM2724 123
IM2725 144
IM2726 144
IM2728 156
IM2729U 156
IM2732 163
IM2732U 163
IM2733 163
IM2733U 163
IM2741 182
IM2742 222
IM2743 83
IM2748 176
IM2750 177
IM2755 173
IM2762 81
IM2764 425
IM2765 425
IM3148 198
IM3169 240
IM3240U 154
IM3264U 136
IM3279U 243
IM3291U 177
IM3292 176
IM3293 242
IM3312 186
IM3324 424
IM3325 424
IM3326 424
IM3327 424
IM3337 176
IM3422 36
IM3448 183
IM3449 183
IM3450 178
IM3451 161
IM3452 161
IM3454U 194
IM3455U 225
IM3456U 152
IM3457U 147
IM3464U 89
IM3465U 89
IM3466U 84
IM3467U 86
IM3468U 88
IM3469U 84
IM3470U 86
IM3471U 88
IM3472U 86
IM3473U 87
IM3474U 85
IM3475U 88
IM3476U 87
IM3477U 87
IM3478U 89
IM3479U 88
IM3480U 85
IM3481U 85
IM3482U 87
IM3483U 89
IM3484U 84
IM3485U 84
IM3486U 86
IM3487U 85
IM3488U 90
IM3489U 90
IM3497 54
IM3514 41
IM3515 41
IM3524 240
IM3524U 240
IM3546 52
IM3548 137
IM3548U 137
IM3549 159
IM3575 182
IM3577 183
IM3578 183
IM3579 156
IM3580 431
IM3600U 157
IM3601U 149
IM3602 232
IM3603 235
IM3604 233
IM3605 147
IM3606U 149
IM3607U 118
IM3608U 108
IM3609U 121
IM3610 99
IM3611 110
IM3613 105
IM3613U 105
IM3614 52
IM3628 117
IM3628U 117
IM3629 118
IM3629U 118
IM3630 47, 48
IM3631 39, 48
IM3632
38, 47, 48
IM3633 108
IM3635 208
IM3636 208
IM3638 163
IM3645 99
IM3646 180
IM3647 180
IM3648 32, 43
IM3657 177
IM3659 424
IM3669U 90
IM3670U 90
IM3698 163
IM3701 146
IM3702 146
IM3703 157
IM3704 120
IM3707 111
IM3709 193
IM3710 193
IM3711 192
IM3716 147
S
S00010 377
S00020 377
S00030 378
S00040 378
S00050 378
S00060 378
S00070 378
S00080 378
S00101 385
S00102 380
S00103 385
S00104 385
S00105 382
S00106 385
S00107 380
S00108 385
S00109 385
S00110 382
S01006 384
S01007 384
S01008 381
S01009 383
S01010 383
S01011 384
S01012 379
S01013 379
S01014 380
S01015 380
S01018 383
S01019 382
S01020 384
S01021 384
S01022 381
S01023 383
S01024 383
S01025 384
S01026 379
S01027 379
S01028 380
S01029 380
S01032 383
S01033 382
S01043 383
S01044 383
S01045 383
S01046 383
S01050 383
S01051 383
S01070 381
S01071 380
S01072 382
S01073 379
S01074 381
S01075 381
S01076 382
S01077 379
S01078 382
S01079 380
S01080 381
S01081 381
S01082 380
S01083 382
S01084 379
S01085 381
S01086 381
S01087 382
447
Part Numbers
S01088 379
S01089 382
S01090 380
S01091 381
S03000 381
S03002 381
S03030 382
S03031 380
S03032 384
S03033 382
S03034 380
S03035 384
T
T01006 361
T01007 361
T01008 363
T01009 359
T01010 360
T01011 361
T01012 362
T01013 362
T01014 362
T01015 362
T01016 361
T01017 363
T01018 360
T01019 363
T01020 361
T01021 361
T01022 363
T01023 359
T01024 360
T01025 361
T01026 362
T01027 362
T01028 362
T01029 362
T01030 361
T01031 363
T01032 360
T01033 363
T01035 359
T01037 359
T01038 360
T01039 360
T01040 360
T01041 360
T01043 359
T01044 363
T01045 358
T01046 359
T01047 360
T01048 360
T01049 372
T01050 359
T01051 359
T01052 360
T01053 360
T01054 363
T01063 361
T01064 362
T01065 362
T01068 361
T01069 361
T01070 361
T02000 364
T02001 364
T02002 364
T02003 364
T02004 364
T02005 364
T02006 365
T02007 365
T02008 365
T02009 365
T02010 365
T02011 365
T02018 365
T02019 365
T02020 365
T02021 365
T02022 365
T02023 365
T02024 364
T02025 364
T02026 364
T02027 364
T02028 364
T02029 364
T02030 364
T02031 364
T02032 365
T02033 365
T02034 365
T02035 365
T02036 366
T02037 366
T02038 366
T02039 366
T02040 365
T02041 365
T02042 365
T02043 365
T02044 364
T02045 364
T02046 364
T02047 364
T02048 364
T02049 364
T02050 371
T02051 371
T02052 370
T02053 370
T02058 371
T02059 371
T02062 370
T02063 370
T02066 370
T02067 370
T03000 368
T03001 366
T03002 368
T03003 366
T03004 368
T03005 368
T03006 366
T03007 366
T03008 368
T03009 368
T03010 366
T03011 366
T03012 366
T03013 366
T03014 368
T03015 368
T03016 367
T03017 367
T03018 367
T03019 367
T03020 368
T03021 368
T03022 367
T03023 367
T03024 367
T03025 367
T03026 367
T03027 367
T03028 367
T03029 367
T04000 369
T04001 369
T04002 369
T04003 369
T04004 369
T04005 369
T04006 369
T04007 369
T04008 369
T04009 369
T04010 369
T04011 369
T04012 369
T04013 369
T04014 369
T04015 369
T06000 376
T06001 376
T06100 375
T06101 376
T06102 376
T06103 376
T06104 376
T06105 376
T06106 376
T06107 376
T06210 376
T08002 374
T08003 374
T08005 373
T09004 374
175474 22
175478 22
175621 53
175651 53
175658 53
177017 38, 45
177018 38, 47
177495 23
342184 27
369292 24
369295 24
369694 27
373661 17, 18
378600 30
383260 22
383550 23
383556 22
383721
17, 18, 22
383722 22
392049 27
392050 27
392051 27
392052 27
392932 24
392933 24
392934 24
393124 25
393125 25
393126 25
393127 25
393128 25
393129 25
393130 25
393131 25
448
Part Numbers
393132 25
393133 25
393134 25
393135 25
623101 17
624922 33
629966
17, 18, 58
629967
17, 18, 58
629968
17, 18, 58
629969
17, 18, 58
629972
17, 18, 58
721532 388
721535 394
721537 394
721539 395
721540 389
731085
17, 18, 58
731086
17, 18, 58
731087
17, 18, 58
731088
17, 18, 58
731576 393
731577 393
731578 393
731580 431
731581 391
731583 391
731584 391
731585 391
731586 391
731588 431
731589 392
731591 392
731592 392
731593 392
731594 392
731596 431
731597 392
731599 392
731600 392
731601 392
731602 392
731604 431
731605 393
731607 393
731608 393
731609 393
731610 393
731613 390
731614 390
731615 390
731616 390
731617 390
731620 390
731621 390
731622 390
731623 390
731624 390
731625 390
731627 391
731628 394
731629 394
731630 394
731631 394
731632 394
731635 388
731636 388
731637 388
731638 388
731639 388
731640 388
731641 388
731642 393
731643 393
731644 393
731645 393
731646 393
731647 393
731648 393
731649 390
731650 391
731651 391
731652 391
731653 391
731656 394
731657 394
731658 394
731659 394
731660 394
731662 392
731663 394
731664 394
731665 394
731666 394
731667 394
731670 394
731671 394
731672 394
731673 394
731674 394
731677 395
731678 395
731679 395
731680 395
731681 395
731684 389
731685 389
731686 389
731687 389
731688 389
731691 388
731692 388
731693 388
731694 392
731696 389
731697 389
731698 389
731699 389
731700 390
731701 389
731702 389
731703 389
731705 388
731706 429
731707 428
731708 428
731709 428
731710 428
731711 428
731712 428
731713 429
731716 269
731718 52
731720 197
731721 197
731722 197
731723 197
731724 197
731727 427
731728 427
731729 279
731730 196
731731 196
731732 279
731733 219
731734 411
731735 411
731736 411
731737 411
731738 411
731739 411
731740 411
731741 411
731742 411
731743 411
731744 219
731745 219
731746 206
731747 206
731748 206
731749 209
731750 209
731751 209
731752 215
731753 215
731754 215
731755 216
731756 216
731757 217
731758 217
731759 217
731760 218
731761 414
731762 414
731763 414
731764 414
731765 414
731767 218
731768 218
731769 218
731770 218
731771 218
731772 220
731773 220
731774 220
731775 220
731776 205
731777 205
731778 205
731779 410
731780 410
731781 410
731782 410
731783 410
731784 410
731785 410
731786 410
731787 410
731788 410
731789 288
731790 288
731791 288
731792 289
731793 289
731794 289
731795 289
731796 289
731797 289
731798 290
731799 290
731800 290
731801 290
731802 410
731803 410
731804 410
731805 410
731806 410
731807 410
449
Part Numbers
731808 410
731809 410
731810 410
731811 290
731812 290
731813 288
731814 288
731815 288
731816 291
731817 291
731818 291
731819 413
731820 413
731821 413
731822 413
731824 300
731825 300
731826 300
731827 280
731828 280
731829 280
731830 284
731831 284
731832 407
731833 407
731834 407
731835 407
731836 407
731837 407
731838 407
731839 407
731840 407
731841 407
731842 407
731843 407
731844 407
731845 407
731846 407
731847 407
731848 407
731849 407
731850 407
731851 407
731852 412
731853 412
731854 412
731855 412
731856 412
731857 412
731859 408
731860 408
731861 408
731862 408
731863 406
731864 406
731865 406
731866 406
731871 411
731872 411
731873 411
731874 411
731875 271
731877 272
731878 272
731882 273
731883 294
731887 294
731889 411
731890 411
731891 411
731892 411
731904 280
731905 299
731907 408
731908 408
731909 408
731910 408
731911 408
731912 408
731913 408
731914 408
731915 408
731916 408
731917 412
731918 412
731919 412
731920 412
731921 409
731922 409
731923 409
731924 409
731925 409
731926 409
731927 409
731928 409
731929 409
731930 409
731931 409
731932 409
731933 409
731934 409
731935 409
731936 413
731937 413
731938 413
731939 413
731940 409
731941 409
731942 409
731943 409
731944 409
731945 409
731946 409
731947 409
731948 409
731949 409
731950 413
731951 204
731952 204
731953 204
731954 427
731955 427
731956 262
731957 262
731958 262
731959 262
731960 262
731963 275
731964 275
731965 275
731966 275
731968 267
731969 267
731970 267
731971 267
731973 249
731975 249
731976 249
731977 249
731978 249
731979 249
731980 249
731981 249
731982 249
731983 249
731986 250
731987 250
731988 250
731989 250
731990 250
731991 250
731992 250
731993 250
731994 250
731995 250
731998 251
731999 251
732000 251
732001 251
732002 251
732003 251
732006 251
732007 252
732008 252
732009 252
732010 252
732011 252
732012 252
732014 252
732015 252
732016 252
732017 252
732018 252
732019 252
732021 253
732022 253
732023 253
732024 253
732025 253
732026 253
732029 253
732030 253
732031 253
732032 253
732033 253
732035 254
732036 254
732037 254
732038 254
732039 254
732040 254
732043 254
732044 254
732045 254
732046 254
732047 254
732048 254
732049 254
732052 255
732053 255
732054 255
732055 255
732056 256
732057 256
732058 256
732059 256
732060 256
732061 256
732064 256
732065 256
732066 256
732067 256
732068 256
732069 256
732071 257
450
Part Numbers
732072 257
732073 257
732074 257
732075 257
732078 257
732079 257
732080 257
732081 257
732083 258
732084 258
732085 258
732086 258
732087 259
732088 259
732089 259
732090 259
732091 259
732092 259
732095 259
732096 259
732097 259
732098 259
732100 260
732101 260
732102 260
732103 260
732104 260
732107 260
732108 260
732109 260
732111 260
732112 260
732113 260
732114 260
732116 261
732117 261
732118 261
732119 261
732121 255
732122 255
732123 255
732124 390
732125 261
732126 261
732127 261
732128 261
732130 261
732131 261
732132 261
732133 261
732134 261
732136 262
732137 262
732138 262
732139 262
732141 278
732142 278
732143 278
732144 278
732146 263
732147 263
732148 263
732149 263
732150 263
732151 263
732153 265
732154 265
732155 265
732156 265
732157 265
732158 265
732161 265
732162 265
732163 265
732164 265
732165 265
732166 265
732167 266
732168 264
732169 264
732170 266
732171 266
732172 267
732173 267
732174 267
732175 267
732176 267
732178 268
732179 268
732180 268
732182 268
732183 268
732184 268
732185 268
732186 268
732188 269
732189 269
732190 269
732191 269
732193 269
732194 269
732195 269
732196 269
732198 270
732199 270
732200 270
732201 270
732202 271
732203 271
732204 271
732205 271
732206 271
732208 272
732209 272
732210 272
732211 272
732212 272
732214 272
732215 272
732216 272
732217 272
732218 272
732219 273
732220 273
732221 273
732222 273
732223 273
732224 273
732227 294
732228 294
732229 294
732230 294
732231 294
732232 294
732233 294
732234 294
732235 280
732236 280
732237 280
732238 280
732239 279
732240 279
732241 299
732242 299
732243 299
732244 299
732246 299
732247 299
732248 299
732249 299
732250 299
732253 278
732254 278
732255 278
732256 278
732258 278
732259 408
732260 264
732261 264
732262 264
732263 264
732264 264
732265 264
732266 264
732267 264
732268 295
732269 295
732270 295
732271 295
732272 296
732273 296
732274 296
732275 296
732276 296
732277 296
732278 296
732279 296
732280 298
732281 298
732282 298
732283 298
732284 298
732285 298
732286 298
732287 298
732289 266
732290 266
732291 266
732292 266
732293 266
732295 291
732296 291
732297 291
732298 291
732300 406
732303 273
732304 273
732305 273
732306 273
732307 274
732308 274
732309 274
732310 274
732311 406
732312 258
732313 258
732314 258
732315 258
732316 411
732317 266
732318 266
732319 266
732320 266
732321 266
732322 271
451
Part Numbers
732323 271
732324 271
732325 271
732326 411
732327 270
732328 270
732329 270
732330 270
732332 275
732334 275
732335 297
732336 297
732337 297
732339 293
732340 293
732341 293
732346 277
732347 277
732348 277
732349 277
732350 277
732351 297
732352 297
732353 297
732354 297
732355 283
732356 283
732357 284
732358 284
732359 284
732360 284
732361 284
732362 284
732363 284
732364 284
732365 295
732366 295
732367 295
732368 413
732369 413
732370 413
732371 410
732372 410
732373 410
732374 410
732375 410
732376 410
732377 410
732378 410
732379 410
732380 410
732381 413
732382 413
732383 413
732384 413
732385 407
732386 407
732387 285
732388 285
732389 285
732390 285
732392 286
732393 286
732394 286
732395 286
732396 286
732397 286
732398 287
732399 287
732400 287
732401 287
732402 287
732403 287
732415 285
732416 285
732417 285
732418 285
732419 285
732420 285
732421 292
732422 292
732423 292
732424 292
732425 292
732426 292
732427 292
732428 293
732429 293
732430 293
732431 293
732432 293
732433 293
732434 292
732435 292
732436 292
732437 292
732438 292
732439 292
732440 286
732441 286
732442 286
732443 286
732444 286
732445 286
732446 287
732447 287
732448 287
732449 287
732450 287
732451 287
732452 430
732453 429
732454 429
732455 430
732456 430
732457 429
732458 429
732459 430
732460 429
732461 430
732462 430
732463 429
732464 429
732465 430
732466 430
732467 429
732468 430
732469 430
732470 430
732471 199
732472 199
732473 304
732474 304
732475 199
732476 199
732477 200
732478 200
732479 200
732480 200
732481 200
732482 200
732483 228
732484 228
732485 228
732486 228
732487 295
732488 295
732490 277
732491 277
732492 299
732493 299
732494 281
732495 281
732496 281
732497 281
732498 282
732499 282
732500 282
732501 282
732502 282
732503 282
732504 282
732505 282
732506 281
732507 281
732508 281
732509 281
732510 282
732511 282
732512 282
732513 282
732514 272
732515 272
732516 272
732517 272
732518 411
732519 283
732520 283
732521 283
732522 283
732523 283
732524 283
732525 283
732526 283
732527 276
732528 276
732529 276
732530 276
732531 276
732532 276
732533 274
732534 274
732535 274
732536 274
732537 274
732538 411
732539 402
732540 402
732541 402
732542 402
732543 402
732544 402
732545 402
732546 402
732547 402
732548 404
732549 404
732550 404
732551 404
732552 401
732553 401
732554 401
732555 401
732556 401
732557 401
452
Part Numbers
732558 401
732559 401
732560 404
732561 404
732562 404
732563 404
732564 411
732565 401
732566 401
732567 401
732568 401
732569 401
732570 401
732571 401
732572 401
732573 403
732574 403
732575 403
732576 403
732577 401
732578 401
732579 401
732580 401
732581 401
732582 401
732583 401
732584 401
732585 401
732586 409
732587 405
732588 405
732589 405
732590 405
732591 403
732592 403
732593 403
732594 403
732595 403
732596 409
732597 403
732598 403
732599 403
732600 403
732601 400
732602 400
732603 400
732604 400
732605 400
732606 400
732607 400
732608 400
732609 403
732610 403
732611 403
732612 403
732613 402
732614 402
732615 402
732616 402
732617 402
732618 402
732619 402
732620 404
732621 404
732622 404
732623 404
732624 404
732625 404
732626 404
732627 404
732628 402
732629 402
732630 402
732631 402
732632 402
732633 402
732634 402
732635 405
732636 405
732637 405
732638 405
732639 405
732640 405
732641 405
732642 405
732643 420
732644 420
732645 420
732646 420
732647 420
732648 420
732649 420
732650 420
732651 420
732652 420
732653 419
732654 419
732655 419
732656 419
732657 419
732658 419
732659 419
732660 418
732661 418
732662 418
732663 418
732664 418
732665 418
732666 418
732667 418
732668 419
732669 419
732670 419
732671 419
732672 419
732673 419
732674 419
732675 419
732676 419
732677 419
732678 420
732679 420
732680 420
732681 420
732683 301
732684 301
732685 301
732686 301
732687 301
732688 301
732689 301
732690 301
732691 301
732692 301
732693 302
732694 302
732695 302
732696 302
732697 302
732698 302
732699 302
732700 302
732702 302
732703 302
732704 302
732705 302
732706 302
732707 302
732708 302
732709 302
732710 302
732711 302
732712 302
732713 302
732714 407
732715 303
732716 303
732717 303
732718 303
732719 303
732720 417
732721 417
732722 417
732723 417
732724 417
732725 417
732726 417
732727 417
732728 417
732729 417
732730 416
732731 416
732732 416
732733 416
732734 416
732735 416
732736 416
732737 416
732738 416
732739 416
732740 416
732741 416
732742 416
732743 416
732744 416
732745 416
732746 416
732747 416
732748 416
732749 416
732750 416
732751 416
732752 416
732755 417
732756 417
732757 417
732758 417
732759 321
732760 321
732761 321
732762 321
732763 320
732764 320
732765 320
732766 320
732767 317
732768 317
732769 317
732770 317
732771 317
732772 317
732773 317
732774 317
732776 317
732777 317
453
Part Numbers
732778 317
732779 317
732780 318
732781 318
732782 318
732783 318
732785 318
732786 318
732787 318
732788 318
732790 319
732791 319
732792 319
732793 319
732794 319
732795 319
732796 319
732797 319
732798 319
732799 319
732800 319
732801 319
732802 431
732809 406
732810 406
732811 406
732812 406
732813 406
732814 406
732815 406
732816 406
732817 422
732818 422
732819 422
732820 422
732825 398
732826 398
732827 398
732828 398
732833 396
732834 396
732835 396
732836 396
732837 397
732838 397
732839 397
732840 397
732841 415
732842 415
732843 415
732844 415
732845 399
732846 399
732847 399
732848 399
732849 399
732850 399
732851 399
732852 399
732853 399
732854 400
732855 400
732856 400
732857 396
732858 396
732859 396
732860 396
732861 397
732862 397
732863 397
732864 397
732865 399
732866 399
732867 399
732868 399
732869 397
732870 397
732871 397
732872 397
732873 422
732874 422
732875 422
732876 422
732877 415
732878 415
732879 415
732880 415
732881 415
732882 415
732883 415
732884 415
732885 421
732886 421
732887 421
732888 421
732889 421
732890 421
732891 421
732892 421
732893 405
732894 405
732895 405
732896 405
732897 405
732898 405
732899 405
732900 405
732901 405
732902 405
732903 422
732904 422
732905 422
732906 422
732907 422
732908 422
732909 422
732910 422
732911 422
732912 422
732913 398
732914 398
732915 398
732916 398
732917 398
732918 398
732919 398
732920 398
732921 398
732922 398
732923 414
732924 414
732925 414
732926 414
732927 414
732928 414
732929 414
732930 414
732931 414
732932 414
732933 421
732934 421
732935 421
732936 421
732937 421
732938 421
732939 421
732940 421
732941 421
732942 421
732943 421
732944 421
732945 421
732946 421
732947 400
732948 400
732949 400
732950 400
732951 400
732953 399
732954 399
732955 399
732956 399
732957 399
732958 399
732959 396
732960 396
732961 396
732962 396
732963 396
732965 406
732966 406
732967 406
732968 406
732969 406
732970 406
732971 398
732972 398
732973 398
732974 398
732975 398
732976 398
732977 418
732978 418
732979 418
732980 418
732981 418
732982 418
732983 415
732984 415
732985 415
732986 415
732987 415
732988 415
732989 424
732990 424
732991 424
732992 424
732993 424
732994 424
732995 424
732996 424
732997 424
732998 424
732999 424
733000 424
733001 424
733002 424
733005 424
733006 423
733007 423
733008 423
733009 423
733010 423
733011 423
733012 423
454
Part Numbers
733013 423
733014 423
733016 423
733017 423
733018 423
733019 423
733020 423
733022 423
733023 423
733024 423
733025 316
733026 316
733027 316
733028 316
733029 316
733030 316
733031 316
733032 316
733033 316
733034 316
733035 316
733036 316
733037 305
733038 305
733039 305
733040 305
733042 305
733043 305
733044 305
733045 305
733046 306
733047 306
733048 306
733049 306
733050 314
733051 314
733052 314
733053 314
733054 314
733055 314
733056 314
733057 314
733058 315
733059 315
733060 315
733061 315
733063 305
733064 305
733065 305
733066 305
733067 307
733068 307
733069 307
733070 307
733071 308
733072 308
733073 308
733074 308
733075 308
733077 307
733078 307
733079 307
733080 307
733081 311
733082 311
733083 311
733084 311
733085 312
733087 312
733088 312
733089 312
733090 312
733091 312
733093 312
733094 312
733095 312
733096 311
733097 311
733098 311
733099 312
733100 312
733101 312
733102 313
733103 313
733104 313
733105 313
733106 309
733107 309
733108 309
733109 309
733110 306
733111 306
733112 306
733113 306
733114 310
733115 310
733116 310
733117 310
733118 310
733119 310
733120 310
733121 310
733122 313
733123 313
733124 313
733125 313
733126 307
733127 307
733128 307
733129 307
733130 309
733131 309
733132 309
733133 309
733134 308
733135 308
733136 308
733137 308
733139 306
733140 306
733141 306
733142 306
733143 311
733144 311
733145 311
733146 311
733147 214
733148 214
733149 214
733150 214
733151 214
733152 214
733153 214
733154 214
733155 214
733156 214
733157 214
733158 210
733159 210
733160 210
733161 210
733164 212
733165 212
733166 212
733167 212
733168 211
733169 211
733170 211
733171 211
733172 211
733173 211
733174 212
733175 212
733176 212
733177 212
733178 212
733179 212
733180 212
733181 212
733182 212
733183 212
733184 212
733185 212
733186 212
733187 212
733188 213
733189 213
733190 213
733191 213
733192 210
733193 210
733194 210
733195 210
733196 210
733197 210
733198 210
733199 210
733200 211
733201 211
733202 211
733203 211
733204 223
733205 223
733206 223
733207 223
733208 223
733209 223
733210 214
733211 214
733212 214
733213 214
733214 214
733215 213
733216 213
733217 213
733218 213
733219 213
733220 213
733221 213
733222 213
733223 213
733230 211
733231 211
733232 211
733233 211
733234 211
733235 285
733236 286
733237 287
733238 287
733241 286
733242 285
733243 292
733244 293
733245 292
455
Part Numbers
733246 286
733247 287
733248 228
733249 262
733250 267
733251 249
733252 249
733253 249
733254 250
733256 250
733257 250
733258 250
733259 251
733262 252
733263 252
733264 253
733267 253
733268 254
733270 254
733271 254
733273 256
733277 257
733278 257
733280 259
733281 259
733282 259
733284 260
733285 260
733286 260
733287 260
733289 260
733290 261
733291 261
733292 255
733293 255
733294 255
733298 261
733300 261
733302 262
733303 262
733307 265
733310 393
733311 393
733321 17
734163 264
734171 264
734179 298
734185 298
734187 266
734193 291
734200 258
734208 258
734236 293
734243 277
734250 277
734264 297
734285 283
734404 283
734555 295
734662 295
734685 283
734697 277
734705 277
734728 299
734734 299
734742 272
734751 403
734763 402
734782 402
734794 404
734799 402
734806 405
734810 420
734815 420
734820 420
734824 419
734829 420
734845 302
734860 416
734877 416
734881 417
734887 417
735025 397
735027 397
735056 415
735062 399
735079 399
735084 399
735099 400
735101 396
735106 421
735110 422
735167 398
735187 421
735213 400
735235 400
735331 424
735907 423
735913 312
735915 311
735921 312
735923 314
735929 314
735936 314
735944 214
735951 214
735972 214
735979 214
735993 210
736000 210
736007 212
736021 211
736291 212
736398 212
736403 213
736408 213
736421 210
736433 210
736441 210
736464 210
736470 213
736478 213
736487 213
736518 52
736556 288
736560 290
736565 266
736571 266
736576 268
736581 249
736596 268
736613 299
736628 390
736633 389
736711 389
736717 390
736730 389
736749 389
736756 389
736761 389
736763 414
736792 414
736798 414
736815 410
736820 410
736830 407
736835 407
736837 412
736842 412
736903 408
736905 408
736918 408
736931 406
736944 406
736949 411
736957 411
736970 411
736981 394
737067 388
737657 101
737659 106
737660 103
737661 106
737662 391
737663 34
737664 36
773875 17
775014 12
775015 12
775016 12
2523749 60
6415063
17, 29, 58
6602137 100
6602381 118
6602385 106
6602389 100
6602393 103
6602398 390
6602431 390
6602434 393
6602463 108
6602507 113
6602573 110
6602626 112
6602689 128
6602705 145
6602764 44
6602796 23
6602864 103
6602868 100
6602872 391
6602876 392
6602884 391
6602888 392
6602906 392
6602928 391
6602940 393
6602985 128
6602989 112
6602993 134
6603001 392
6603012 117
6603020 104
6603024 117
6603034 392
6603038 392
6603042 128
6603067 145
6603109 125
6603113 125
6603117 140
6603152 44
6603177 125
6603181 140
6603185 99
6603192 123
456
Part Numbers
6603196 119
6603200 123
6603262 113
6603266 113
6603274 106
6603369 427
6603424 209
6603446 118
6603473 35
6603475 108
6603482 390
6603511 113
6603795 76
6603796 75
6603798 75
6603799 74
6603800 74
6603801 75
6603802 74
6603822 105
6603824 105
6603825 105
6603827 105
6603838 138
6603839 138
6603846 117
6603848 106
6603849 100
6603850 103
6603852 392
6603853 392
6603855 392
6603857 145
6603858 118
6603859 117
6603861 106
6603862 103
6603863 100
6603864 391
6603866 140
6603876 49
6603877 393
6603904 140
6603909 75
6603914 76
6604104 138
6604105 125
6604106 118
6604107 108
6604110 113
6604114 113
6604117 393
6604119 392
6604120 108
6604121 128
6604159 125
6604238 76
6604239 73
6604240 76
6604241 75
6604248
37, 45, 46
6604366 209
6604368 121
6604422 123
6604426 121
6604428 120
6604500 113
6604548 391
6604551 118
6604614 74
6604621 101
6604623 101
6604625 101
6604627 101
6604665 207
6604667 207
6604700 392
6604727 103
6604728 106
6604894 115
6604928 132
6604929 73
6604930 73
6604931 165
6605015 73
6605359
17, 18, 34, 45,
46, 47, 48, 58
6605429 29
6605698 23
6605699 23
6605700 23
6607007
35, 45, 46, 47,
48
6607010 101
6607011 106
6607012 391
6607013
45, 46, 71
6607015 71
6607017 72
6607019 72
6607021 37, 46
6607023
36, 45, 46, 47,
48
6607053 71
6607054 73
6607068 74
6607071 72
6607072 72
6607073
45, 46, 71
6607077
37, 45, 46
6607098
37, 45, 46
6607099 391
6607100 101
6607101 103
6607102 106
6607103 77
6607107 124
6607108 124
6607109 107
6607110 151
6607111 124
6607112 123
6607115 134
6607116 134
6607117 134
6607118 115
6607120 34
6607121 36
6607123 107
6607130 43
6607131 43
6912932 20, 58
6913337 20, 58
6915048
19, 49, 58
6915056 49
6915115 29
6915493
20, 50, 58
6915549 30
7546946 39, 40
7546999 29, 40
7547053
35, 45, 46
7547059 49
7800091 23
8320310 23
8320312 23
8546719 59
8546859 59
8546929 59
8546930 59
8546931 59
8547008 59
8547198 60
9912784 23
9912785 23
9912786 23
9912787 23
9912788 23
9913155 23
9913156 23
9913157 23
457
Product Name Target Page Product Product Name Target Page Product
Alphabetical
2B4
Human Antibody
186
6C3
Mouse Antibody
280
7-AAD kit
Human System
52
7-AAD Viability Dye
Quality Control
36
7.1 (NG2 antigen)
Human Antibody
194
7A6
Human Antibody
195
A
A
b
Mouse Antibody
283
ABTS substrate
Support Reagent
428
Accessories for Vi-CELL
Instrument
22
Accuvettes
Accessory
23
AEC
Detection Kit
426
AIM
Human Antibody
151
AIRM1
Human Antibody
228
Akt
Human Antibody
194
Human Cell Lab
325
ALCAM
Human Antibody
179
ALK
Human Antibody
186
Allegra X-15R
Instrument
24
Allergenicity Kit
System
50
aminopeptidase N
Human Antibody
112
Annexin V
Human System
52
APC (675/633) Set-up Kit
Fluorosphere
34
Aperture Concentration Control
Instrument
23
APO-1
Human Antibody
158
APO2.7
Human Antibody
195
Apoptosis detection kit
Human System
51
Apoptotic cells
Human Antibody
195
ApoScreen Propidium Iodide
Support Reagent
427
Avanti J-26 XP Series
Instrument
25
Avidin
Streptavidin
423
B
B7-1
Human Antibody
153
Mouse Antibody
271
B7-2
Human Antibody
156
Mouse Antibody
272
B29
Human Antibody
152
Mouse Antibody
270
B220
Mouse Antibody
265
B-Cell Subset
Pig Antibody
319
B cells
Human Antibody
205
B4
Human Antibody
117
B7-DC
Human Antibody
187
B7-H1
Human Antibody
188
Bad
Human Antibody
196
Bax
Human Antibody
196
Mouse Antibody
279
BCIP substrate
Support Reagent
428
BCIP/NBT substrate solution
Support Reagent
428
Bcl-2
Human Antibody
196
Mouse Antibody
279
Bcl-xL
Human Antibody
197
Human Cell Lab
327
Biomek NX
P
Cell Preparation
32
Biomek 3000
Cell Preparation
31
BiP
Mouse Antibody
279
BLAST-1
Human Antibody
141
BL-CAM
Human Antibody
120
Bovine Type I Collagen
Support Reagent
429
Bovine Type II Collagen
Support Reagent
429
Bovine Type III Collagen
Support Reagent
429
Bovine Type IV Collagen
Support Reagent
429
Bovine Type V Collagen
Support Reagent
429
Bovine Type VI Collagen
Support Reagent
430
BP-1
Mouse Antibody
280
Bp35
Human Antibody
118
BST-1
Human Antibody
173
BTLA
Human Antibody
187
Bu-1
Chicken Antibody
309
Bu-1a
Chicken Antibody
310
Bu-1b
Chicken Antibody
310
BY55
Human Antibody
177
C
C1.7
Human Antibody
186
C3bi receptor
Human Antibody
110
C3bi, C3dg receptor
Human Antibody
111
C3d receptor
Human Antibody
119
C-kit
Mouse Antibody
277
Human Antibody
162
Chicken Antibody
310
Human Cell Lab
328
Cadherin 5
Human Antibody
171
458
Alphabetical
CALLA
Human Antibody
108
Canted-Neck Flask Adapters
Instrument
24
Caspase 8
Human Antibody
198
CD1
Pig Antibody
317
CD1a
Human Antibody
99
CD1c
Human Antibody
99
CD2
Human Antibody
100
Mouse Antibody
249
CD2-FITC/CD7-PE
Human Multi-Color
73
CD2-FITC/CD19-PE
Human Multi-Color
77
CD3
Chicken Antibody
305
Human Antibody
101
Rat Multi-Color
248
Non-Human Multi-Color
247
Non-Human
247
CD3-FITC/CD4-PE
Human Multi-Color
73
Human Multi-Color
78
Rat Multi-Color
248
Non-Human Multi-Color
247
Human Multi-Color
91
CD3-FITC/CD8-PE
Human Multi-Color
73
Human Multi-Color
78
Human Multi-Color
92
Human Multi-Color
91
CD3-FITC/CD16-PE
Human Multi-Color
78
CD3-FITC/CD(16+56)-PE
Human Multi-Color
78
CD3-FITC/CD19-PE
Human Multi-Color
73
Human Multi-Color
78
Human Multi-Color
91
CD3-FITC/CD25-PE
Human Multi-Color
79
CD3-FITC/CD45RA-PC7/CD161a-
APC
Rat Multi-Color
248
CD3-FITC/CD56-PE
Human Multi-Color
74
Human Multi-Color
79
CD3-FITC/CD56-PC5
Human Multi-Color
79
CD3-FITC/HLA-DR-PE
Human Multi-Color
79
Human Multi-Color
93
CD3-FITC/HLA-DR-PE
Human Multi-Color
79
Human Multi-Color
93
CD3
Pig Antibody
317
Mouse Antibody
249
CD4
Chicken Antibody
305
Mouse Antibody
251
Human Antibody
103
Feline Antibody
316
CD4 Count Kit
System
49
CD4-FITC/CD8-PE
Human Multi-Color
74
Human Multi-Color
80
CD4/CD8
Human Multi-Color
93
Human Multi-Color
91
CD4-FITC/CD29-PE
Human Multi-Color
74
CD4-FITC/CD45RA-PE
Human Multi-Color
74
CD4a
Pig Antibody
318
CD5
Human Antibody
104
Mouse Antibody
252
Feline Antibody
316
Chicken Antibody
306
Human Multi-Color
84
Human Multi-Color
84
Human Multi-Color
84
CD6 ligand
Human Antibody
179
CD7
Human Antibody
105
CD8
Human Antibody
106
Feline Antibody
316
CD8
Chicken Antibody
306
Pig Antibody
318
Mouse Antibody
253
CD8
Human Antibody
107
Chicken Antibody
307
CD8-FITC/CD4-PE
Human Multi-Color
74
Human Multi-Color
80
Human Multi-Color
84
Human Multi-Color
71
Human Multi-Color
77
CD8-FITC/CD38-PE
Human Multi-Color
80
CD8-FITC/CD56-PE
Human Multi-Color
75
CD9
Human Antibody
108
CD10
Human Antibody
108
CD10-FITC/CD19-PE
Human Multi-Color
75
CD11a
Human Antibody
109
Mouse Antibody
253
CD11b
Human Antibody
110
Mouse Antibody
254
CD11c
Human Antibody
111
459
Alphabetical
CD13
Human Antibody
112
CD14
Human Antibody
113
Human Multi-Color
85
Human Multi-Color
85
CD(14+16)-FITC/CD85k-PE/CD33-
PC5
Human Multi-Color
77
CD(14+16)-FITC/CD85k-PE/CD123-
PC5
Human Multi-Color
77
CD15
Human Antibody
114
Human Multi-Color
85
CD16
Human Antibody
115
CD16/32
Mouse Antibody
255
Human Multi-Color
85
CD16b
Human Antibody
115
CD18
Human Antibody
116
Mouse Antibody
255
CD19
Mouse Antibody
256
Human Antibody
117
CD19-FITC/CD2-PE
Human Multi-Color
75
CD19-FITC/CD5-PE
Human Multi-Color
80
Human Multi-Color
86
CD20
Human Antibody
118
Human Antibody
205
Human Multi-Color
86
CD21
Human Antibody
119
Pig Antibody
319
CD22
Human Antibody
120
Mouse Antibody
257
CD23
Mouse Antibody
257
Human Antibody
121
CD24
Human Antibody
122
Mouse Antibody
258
CD25
Human Antibody
123
Mouse Antibody
259
Human Multi-Color
86
Human Multi-Color
86
CD26
Human Antibody
123
CD27
Human Antibody
124
CD28
Human Antibody
124
Mouse Antibody
260
Chicken Antibody
307
CD29
Human Antibody
125
CD30
Human Antibody
126
Mouse Antibody
260
CD31
Human Antibody
126
Mouse Antibody
261
CD32
Human Antibody
127
CD33
Human Antibody
128
CD34
Human Antibody
130
Human Multi-Color
87
Human Multi-Color
87
CD35
Human Antibody
130
CD36
Human Antibody
131
CD36-FITC/CD235a-PE
Human Multi-Color
80
CD37
Human Antibody
131
CD38
Human Antibody
132
Mouse Antibody
261
CD38-FITC/CD8-PE
Human Multi-Color
81
Human Multi-Color
87
CD40
Human Antibody
133
Mouse Antibody
262
CD40L
Human Antibody
172
Mouse Antibody
278
CD41
Human Antibody
134
CD41-FITC/CD235a-PE/CD45-ECD
Human Multi-Color
87
CD42a
Human Antibody
135
CD42b
Human Antibody
135
CD43
Human Antibody
136
CD44
Human Antibody
136
Mouse Antibody
262
Chicken Antibody
308
Human Cell Lab
324
CD45
Human Antibody
138
Mouse Antibody
263
Rat Antibody
301
Chicken Antibody
308
CD45.1
Mouse Antibody
264
CD45.2
Mouse Antibody
264
CD45-FITC/CD4-PE/CD8-ECD/CD3-
PC5
Human Multi-Color
71
Human Multi-Color
71
Human Multi-Color
72
CD45-FITC/CD14-PE
Human Multi-Color
75
Human Multi-Color
81
CD45/CD14
Human Multi-Color
93
Human Multi-Color
72
Human Multi-Color
72
460
Alphabetical
CD45-FITC/CD56-PE/CD19-
ECD/CD3-PC5
Human Multi-Color
71
CD45-FITC/CD235a-PE
Human Multi-Color
81
CD45-FITC/IgG1-PE/IgG1-PC5
Human Multi-Color
72
CD45R
Mouse Antibody
265
CD45R0
Human Antibody
139
CD45RA
Human Antibody
140
CD45RA-FITC/CD4-PE
Human Multi-Color
81
CD45RB
Mouse Antibody
265
CD45RC
Mouse Antibody
266
Rat Antibody
301
CD46
Human Antibody
141
CD48
Human Antibody
141
CD49b
Human Antibody
141
Mouse Antibody
266
CD49c
Human Antibody
142
CD49d
Mouse Antibody
267
Human Antibody
142
CD49e
Mouse Antibody
267
Human Antibody
142
CD49f
Human Antibody
143
CD50
Human Antibody
143
CD51
Human Antibody
143
CD54
Human Antibody
144
Mouse Antibody
268
CD55
Human Antibody
144
CD56
Human Antibody
145
CD57
Human Antibody
146
CD57-FITC/CD8-PE
Human Multi-Color
81
CD58
Human Antibody
146
CD58 Ligand
Human Antibody
100
CD59
Human Antibody
147
CD61
Human Antibody
147
CD62L
Human Antibody
148
Mouse Antibody
268
CD62P
Human Antibody
148
CD62P ligand
Human Antibody
178
CD63
Human Antibody
149
CD64
Human Antibody
149
CD65
Human Antibody
150
CD66b
Human Antibody
150
CD69
Human Antibody
151
Mouse Antibody
269
CD70 ligand
Human Antibody
124
CD71
Human Antibody
151
Mouse Antibody
269
Human Multi-Color
88
CD72.1
Mouse Antibody
270
CD77
Human Antibody
151
CD79a
Human Antibody
152
CD79b
Human Antibody
152
Mouse Antibody
270
CD80
Human Antibody
153
Mouse Antibody
271
CD80/CD86 Ligand
Human Antibody
124
CD81
Human Antibody
153
Mouse Antibody
271
CD83
Human Antibody
154
CD85a
Human Antibody
154
CD85d
Human Antibody
155
CD85j
Human Antibody
155
CD85k
Human Antibody
156
CD86
Human Antibody
156
Mouse Antibody
272
CD89
Human Antibody
157
CD90
Human Antibody
157
Mouse Antibody
272
CD90.2
Mouse Antibody
273
CD94
Human Antibody
158
Mouse Antibody
273
CD95
Human Antibody
158
CD102
Mouse Antibody
274
CD103
Human Antibody
159
Mouse Antibody
274
Human Multi-Color
88
CD104
Mouse Antibody
275
CD105
Human Antibody
159
Mouse Antibody
275
CD106
Human Antibody
160
Mouse Antibody
275
CD107a
Mouse Antibody
276
CD107b
Mouse Antibody
276
CD109
Human Antibody
160
CD111
Human Antibody
161
461
Alphabetical
CD112
Human Antibody
161
CD116
Human Antibody
162
CD117
Human Antibody
162
Mouse Antibody
277
CD118
Human Antibody
163
CD120a
Human Antibody
164
CD120b
Human Antibody
164
CD122
Human Antibody
165
Mouse Antibody
277
CD123
Human Antibody
165
CD124
Human Antibody
166
CDw125
Human Antibody
217
CD126
Human Antibody
166
CD127
Human Antibody
167
CD130
Human Antibody
168
CD134
Human Antibody
169
CD135
Human Antibody
169
CD136
Human Antibody
170
CD138
Human Antibody
170
CD144
Human Antibody
171
CD146
Human Antibody
171
CD152
Human Antibody
172
Mouse Antibody
278
CD154
Human Antibody
172
Mouse Antibody
278
CD155
Human Antibody
173
CD157
Human Antibody
173
CD158a,h
Human Antibody
174
CD158b1/b2,j
Human Antibody
175
CD158e1/e2
Human Antibody
176
CD158i
Human Antibody
176
CD159a
Human Antibody
177
CD160
Human Antibody
177
CD161
Human Antibody
178
CD162
Human Antibody
178
CD162R
Human Antibody
178
CD166
Human Antibody
179
CD178
Human Antibody
179
CD179a
Human Antibody
180
CD184
Human Antibody
181
CD203c
Human Antibody
182
CD206
Human Antibody
182
CD207
Human Antibody
183
CD208
Human Antibody
183
CD209
Human Antibody
184
CD226
Human Antibody
184
CD235a
Human Antibody
185
CD243
Human Antibody
185
CD244
Human Antibody
186
CD246
Human Antibody
186
CD247
Human Antibody
240
CD272
Human Antibody
187
CD273
Human Antibody
187
CD274
Human Antibody
188
CD278
Human Antibody
188
CD279
Human Antibody
189
CD294
Human Antibody
190
CD300a
Human Antibody
190
CD309
Human Antibody
191
CD314
Human Antibody
191
CD324
Human Antibody
192
CDw328
Human Antibody
228
CD335
Human Antibody
192
CD336
Human Antibody
193
CD337
Human Antibody
193
c-erbB-2
Human Antibody
198
ChT1
Chicken Antibody
311
c-Kit
Mouse Antibody
277
Human Antibody
162
Chicken Antibody
310
Human Cell Lab
328
Class I iTAg MHC Tetramers
Tetramer
361
Class I Streptamer
Streptamer
385
Class II iTAg MHC Tetramer
Human Tetramer
371
Cleaning Solution Kit, 4 x 10 mL
Reagent
58
Cleaved Caspase-3 (Asp175)
Cell Lab
329
Collagen
Bovine Support Reagent
429
Collagen Type I
Human Antibody
199
Collagen Type II
Bovine Antibody
304
Collagen Type III
Human Antibody
199
Collagen Type IV
Human Antibody
200
462
Alphabetical
Collagen Type V
Human Antibody
200
Collagen Type VI
Human Antibody
200
Collagenase Type 5
Human Antibody
225
Collagenase-1
Human Antibody
224
Corning* T-25 cm
2
Instrument
24
COULTER CLENZ
Instrument
59
COULTER Manual CD4 Positive
Count Kit
System
49
COULTER Stem-Trol Control Cells
Quality Control
38
COX IV
Human Cell Lab
330
CR1
Human Antibody
130
CR2
Human Antibody
119
CRTH2
Human Antibody
190
CTLA-4
Human Antibody
172
Mouse Antibody
278
CXCR4
Human Antibody
181
CXP Analysis Software Packages
Software
17
CyAn ADP
Instrument
14
Cyclin A2
Human Antibody
201
Cyclin B1
Human Cell Lab
331
Cyclin D1
Human Antibody
201
CYTO-COMP Cell Kit
Quality Control
36
Quality Control
37
Quality Control
37
Cytokeratin
Human Antibody
202
Cytokeratins-large spectrum
Human Antibody
202
Cytotoxic lymphocytes
Human Antibody
242
cMPO-FITC/cCD79a-PE/cCD3-ECD
Human Multi-Color
89
D
DAB
Detection Kit
426
DAP12
Human
203
DC-LAMP
Human Antibody
183
DC-SIGN
Human Antibody
184
Decay Accelerating Factor
Human Antibody
144
DIABLO
Human Antibody
229
Dipeptidyl peptidase IV
Human Antibody
123
DNA Reference Calibrator, 5 mL
Reagent
58
DNAM-1
Human Antibody
184
DNAX
Human Antibody
203
Donkey Anti-Goat IgG (H+L)
Goat Secondary
398
Donkey Anti-Mouse IgG (H+L)
Mouse Secondary
406
Donkey Anti-Rabbit IgG (H+L)
Rabbit Secondary
415
Donkey Anti-Rat IgG (H+L)
Rat Secondary
418
E
E-cadherin
Human Antibody
192
Human Cell Lab
332
EBV receptor
Human Antibody
119
Endoglin
Human Antibody
159
Mouse Antibody
275
E-NPP3
Human Antibody
182
EPICS XL-MCL Upgrade Kit
Instrument
58
EPICS XL PSC Quick Scan Bar Code
Gun
Instrument
58
EPICS XL 4th Color Upgrade Kit
Instrument
58
Instrument
50
Estrogen Receptor
Human Antibody
203
F
FADD
Human Antibody
204
Fas
Human Antibody
158
Fas Associated protein with Death
Domain
Human Antibody
204
Fas L
Human Antibody
179
FC 500 MPL with MXP Software
Instrument
17
FC 500 with Carousel Loader and
CXP Software
Software
16
FC 500 with CXP Software
Software
18
Fc Receptor Saturation Reagent
Support Reagent
431
FcR
Human Antibody
157
FcRIIIA/IIIB
Human Antibody
115
FcR
Mouse Antibody
255
FcRI
Human Antibody
149
FcRII
Human Antibody
127
FcRIIIB
Human Antibody
115
FcRII
Human Antibody
121
Mouse Antibody
257
FETALtrol
Human Kit
56
Fibrillin
Human Antibody
204
Fibronectin
Goat Support Reagent
427
Human Antibody
204
Filter tip
Accessory
58
Filter, 675 BP
Instrument
49
Fixative Solution
Lysing Solution
41
FLICE
Human Antibody
198
463
Alphabetical
Fluorosphere
34
Flow-Check Pro Fluorospheres
Fluorosphere
34
Fluorosphere
35
Fluorosphere
35
Flow-Set Pro Fluorospheres
Fluorosphere
35
flt3/flk2
Human Antibody
169
Fluoromount-G*, slide mounting
Support Reagent
431
Fluorospheres
Fluorosphere
35
FMC7
Human Antibody
205
FMC7-FITC/CD23-PE/CD19-ECD
Human Multi-Color
88
FP 1000
Instrument
18
Cell Preparation
33
fucoganglioside type II
Human Antibody
150
Fusin
Human Antibody
181
G
Gallios
Instrument
12
Gb3
Human Antibody
151
G-CSF
Human Antibody
205
Gelatinase A
Human Antibody
224
Gelatinase B
Human Antibody
225
Glycophorin A
Human Antibody
185
GM-CSF
Human Antibody
206
Mouse Antibody
280
GM-CSFR
Human Antibody
162
GMP140
Human Antibody
148
Goat Anti-Bovine IgG (H+L)
Bovine Secondary
396
Goat Anti-Canine IgG (H+L)
Canine Secondary
396
Goat Anti-Chicken IgG (H+L)
Chicken Secondary
397
Goat Anti-Equine IgG (H+L)
Equine Secondary
397
Goat Anti-Feline IgG (H+L)
Feline Secondary
397
Goat Anti-Guinea Pig IgG (H+L)
Guinea Pig Secondary
399
Goat Anti-Human IgA
Human Secondary
400
Goat Anti-Human IgD
Human Secondary
401
Goat Anti-Human IgG
Human Secondary
401
Goat Anti-Human IgM
Human Secondary
401
Goat Anti-Human IgM + IgG + IgA
(H+L)
Human Secondary
402
Goat Anti-Human Kappa
Human Secondary
402
Goat Anti-Human Lambda
Human Secondary
402
Goat Anti-Mouse IgA
Mouse Secondary
406
Goat Anti-Mouse IgE
Mouse Secondary
407
Goat Anti-Mouse IgG
Mouse Secondary
407
Goat Anti-Mouse IgG (H+L)
Mouse Secondary
407
Mouse Secondary
408
Goat Anti-Mouse IgG2a
Mouse Secondary
409
Goat Anti-Mouse IgG2b
Mouse Secondary
409
Goat Anti-Mouse IgG2c
Mouse Secondary
409
Mouse Secondary
410
Goat Anti-Mouse IgM
Mouse Secondary
410
Goat Anti-Mouse IgM + IgG + IgA (H
+L)
Mouse Secondary
411
Goat Anti-Mouse Kappa
Mouse Secondary
411
Goat Anti-Mouse Lambda
Mouse Secondary
411
Goat Anti-Porcine IgG (H+L)
Porcine Secondary
415
Goat Anti-Rabbit IgG
Rabbit Secondary
416
Goat Anti-Rabbit IgG (H+L)
Rabbit Secondary
416
Goat Anti-Rabbit IgM
Rabbit Secondary
416
Goat Anti-Rabbit IgM + IgG (H+L)
Rabbit Secondary
417
Goat Anti-Rat IgG
Rat Secondary
418
Goat Anti-Rat IgG (Fc)
Rat Secondary
418
Goat Anti-Rat IgG (H+L)
Rat Secondary
419
Goat Anti-Rat IgM
Rat Secondary
419
Goat Anti-Rat IgM + IgG (H+L)
Rat Secondary
420
Goat Anti-Rhesus IgG (H+L)
Rhesus Secondary
421
Goat Anti-Hamster IgG (H+L)
Syrian Secondary
399
Goat Anti-Turkey IgG (H+L)
Turkey Secondary
422
Goat F(ab)
2
Anti-Human IgA
Human Secondary
403
Goat F(ab)
2
Anti-Human IgD
Human Secondary
403
Goat F(ab')
2
Anti-Human IgG (Fc)
Human Secondary
403
Goat F(ab)
2
Anti-Human IgG
Human Secondary
403
Goat F(ab')
2
Human Secondary
404
Goat F(ab)
2
Anti-Human IgM
Human Secondary
404
Goat F(ab)
2
Anti-Human IgM + IgG
+ IgA (H+L)
Human Secondary
404
Goat F(ab)
2
Anti-Human Kappa
Human Secondary
404
Goat F(ab)
2
Anti-Human Lambda
Human Secondary
405
Goat F(ab')
2
Anti-Mouse IgG (Fc)
Mouse Secondary
412
Goat F(ab)
2
Mouse Secondary
412
Goat F(ab)
2
Anti-Mouse IgG1
Mouse Secondary
412
Goat F(ab)
2
Anti-Mouse IgG2a
Mouse Secondary
413
Goat F(ab)
2
Anti-Mouse IgG2b
Mouse Secondary
413
464
Alphabetical
Goat F(ab)
2
Anti-Mouse IgG3
Mouse Secondary
413
Goat F(ab)
2
Anti-Mouse IgM
Mouse Secondary
413
Goat F(ab')
2
Anti-Mouse IgM + IgG
(H+L)
Mouse Secondary
413
Goat F(ab)
2
Anti-Mouse IgM + IgG +
IgA (H+L)
Mouse Secondary
414
Goat F(ab)
2
Anti-Rabbit IgG (H+L)
Rabbit Secondary
417
Goat F(ab)
2
Anti-Rat IgG (H+L)
Rat Secondary
420
Goat F(ab)
2
Anti-Hamster IgG (H+L)
Syrian Secondary
400
Goat Fab Anti-Human IgG
Human Secondary
405
Goat Fab Anti-Rat IgM + IgG + IgA
(H+L)
Rat Secondary
420
Goat Fibronectin
427
Goat Serum
431
gp130 transducer chain
Human Antibody
168
gpIb
Human Antibody
135
gpIIa
Human Antibody
125
gpIIa'
Human Antibody
126
gpIIb
Human Antibody
134
gpIIIa
Human Antibody
147
gpIV
Human Antibody
131
gpIa
Human Antibody
141
gpIX
Human Antibody
135
GR-1
Mouse Antibody
295
Granulocytes
Mouse Antibody
295
GSK-3
Human Antibody
206
GSK-3
Human Antibody
207
gTox Flow
Mouse System
246
H
HLA-B27-FITC/HLA-B7-PE
Human Multi-Color
82
H-2D
b
Mouse Antibody
281
H-2D
d
Mouse Antibody
282
H-2D
k
Mouse Antibody
282
H-2K
d
/H-2D
d
Mouse Antibody
282
H-2K
k
Mouse Antibody
283
HA-Tag
Human Cell Lab
333
HB15
Human Antibody
154
H-CAM
Human Antibody
136
Heat Stable Antigen
Mouse Antibody
258
Human Antibody
122
HER2
Human Antibody
198
HIV-1 core antigen
Human Antibody
207
HLA-ABC
Human Antibody
207
HLA-DQ
Human Antibody
207
HLA-DR
Human Antibody
208
HLA-DR, DP, DQ
Human Antibody
209
Human Multi-Color
88
HLA-DR-FITC/CD3-PE
Human Multi-Color
82
Human Multi-Color
89
Human Multi-Color
89
HLA-DR-FITC/CD8-PE
Human Multi-Color
82
HML-1
Human Antibody
159
HNK-1
Human Antibody
146
HPC Enumeration Kit
System
47
HSA
Human Antibody
122
Mouse Antibody
258
HSP70
Human Cell Lab
334
Human Collagen
Human Support Reagent
430
Human Type I Collagen
430
Human Type III Collagen
430
Human Type IV Collagen
430
Human Type V Collagen
430
HyperCyt Autosampler
Cell Preparation
15
HyperCyt 48 Peristaltic Tubing 18
stop
Accessory
15
HyperCyt 8 inch probe
Accessory
15
HyperCyt Ferrule- Clear Plastic
Accessory
15
HyperCyt Software Interface CyAn
Accessory
15
HyperView Offline Experiment
Designer and data Analysis
Accessory
15
I
IOTest Mark TCR V Repertoire
Kit
System
54
Ia
Chicken Antibody
311
I-A
b
Mouse Antibody
283
I-A
d
Mouse Antibody
283
I-E
k
Mouse Antibody
284
ICAM-1
Human Antibody
144
Mouse Antibody
268
ICAM-2
Mouse Antibody
274
ICAM-3
Human Antibody
143
ICOS
Human Antibody
188
IFN-
Mouse Antibody
284
465
Alphabetical
IFN Kit
Tetramer
373
IgA
Mouse Secondary
285
Chicken Antibody
311
Ig
Human Antibody
152
IgA Kappa
Human Isotypic Control
389
IgA Lambda
389
IgA1
Human Antibody
210
IgA2
Human Antibody
210
Ig
Human Antibody
152
Mouse Antibody
270
IgD
Human Antibody
210
Mouse Antibody
285
IgE
Mouse Antibody
286
IgE (D2)
Human Antibody
211
IgE (Fc)
Human Antibody
211
IgG
Human Antibody
211
Chicken Antibody
312
Sheep
395
IgG (H+L)
Human Antibody
389
IgG (canine)
Canine Isotypic Control
388
IgG (Fc)
Human Antibody
212
IgG (goat)
Goat Isotypic Control
388
IgG (hamster)
Hamster Isotypic Control
389
IgG (mouse)
Mouse Isotypic Control
390
IgG (Rabbit)
Rabbit Isotypic Control
393
IgG (rat)
Rat Isotypic Control
394
IgG (rhesus monkey)
Rhesus Isotypic Control
390
IgG1
Human Antibody
212
Rat Antibody
301
IgG1 (mouse)
391
IgG1 (rat)
394
IgG1 Kappa
389
IgG1 Lambda
390
IgG1-FITC/IgG1-PE
Human Multi-Color
75
Human Multi-Color
82
IgG1/IgG1
Human Multi-Color
93
IgG1-FITC/IgG1-PE/CD45-PC5
Human Multi-Color
92
IgG1/IgG1/IgG1
Human Multi-Color
73
Human Multi-Color
92
IgG1-FITC/IgG2a-PE
Human Multi-Color
94
Human Multi-Color
82
IgG1-FITC/IgG2b-PE
Human Multi-Color
76
IgG1-FITC/IgM-PE
Human Multi-Color
76
IgG2 (Fc)
Human Antibody
212
IgG2 (Fd)
Human Antibody
213
IgG2a (mouse)
392
IgG2a
394
Rat Antibody
302
IgG2a-FITC/IgG1-PE
Human Multi-Color
83
Human Multi-Color
76
IgG2a-FITC/IgG2a-PE
Human Multi-Color
83
IgG2a-FITC/IgG2b-PE
Human Multi-Color
76
IgG2b
392
Rat Antibody
302
IgG2b (mouse)
Mouse Antibody
286
IgG2b (rat)
394
IgG2c
Rat Antibody
302
IgG3
Human Antibody
213
Mouse Antibody
287
IgG3 (mouse)
393
IgG4 (Fc)
Human Antibody
213
IgG4 (pFc')
Human Antibody
214
IgM
Chicken Antibody
312
IgM
Rat Antibody
302
Human Antibody
214
Mouse Antibody
287
IgM (mouse)
393
IgM (rabbit)
Rabbit Isotypic Control
393
IgY
Chicken Isotypic Control
388
IL-2
Human Antibody
215
Mouse Antibody
288
IL-2R
Mouse Antibody
277
IL-2R -chain
Human Antibody
165
IL-2R
Mouse Antibody
259
Human Antibody
123
IL-2R
Mouse Antibody
259
Human Antibody
123
IL-3
Human Antibody
215
Mouse Antibody
288
IL-3R
Human Antibody
165
IL-4
Human Antibody
216
Mouse Antibody
289
IL-4R
Human Antibody
166
IL-5
Mouse Antibody
289
Human Antibody
217
466
Alphabetical
IL-5R
Human Antibody
217
IL-6
Human Antibody
218
Mouse
290
IL-6R
Human Antibody
166
IL-6R
Human Antibody
168
IL-7
Human Antibody
218
IL-7R
Human Antibody
167
IL-10
Human Antibody
219
Mouse Antibody
290
IL-13
Human Antibody
220
IL-17
Mouse Antibody
291
ILT2
Human Antibody
155
ILT3
Human Antibody
156
ILT4
Human Antibody
155
ILT5
Human Antibody
154
iMASC Antibody Gating Kit
Tetramer
374
IMMUNO-BRITE Fluorospheres
Fluorosphere
35
IMMUNO-TROL
TM
Cells
Quality Control
37
Quality Control
37
Lysing Solution
40
Instrument Manager
Instrument
18
Integrin 2 chain
Human Antibody
141
Integrin 3 chain
Human Antibody
142
Integrin 4 chain
Human Antibody
142
Mouse Antibody
267
Integrin 5 chain
Human Antibody
142
Mouse Antibody
267
Integrin 6 chain
Human Antibody
143
Integrin E chain
Human Antibody
159
Integrin
IEL
Mouse Antibody
274
integrin V chain
Human Antibody
143
integrin IIb chain
Human Antibody
134
integrin L-chain
Human Antibody
109
integrin M-chain
Human Antibody
110
integrin X-chain
Human Antibody
111
integrin 1 chain
Human Antibody
125
Integrin 2-chain
Human Antibody
116
Mouse Antibody
255
Integrin 3 chain
Human Antibody
147
Interferon
Mouse Antibody
284
Human Antibody
209
interleukin-2
Human Antibody
215
Mouse Antibody
288
interleukin-2 receptor
Human Antibody
165
interleukin-4
Mouse Antibody
289
Human Antibody
166
Human Antibody
166
interleukin-6 receptor beta
Human Antibody
168
Human Antibody
167
Intermediate filament
Human Antibody
244
IntraPrep Permeabilization Reagent
Lysing Solution
40
IOTest 3 Lysing Solution
Lysing Solution
41
IOTest 3 Fixative Solution
Lysing Solution
41
IRp60
Human Antibody
190
Iso-Diluent
Reagent
58
IsoFlow Sheath Fluid
Instrument
59
ISOTON II
Instrument
59
iTag MHC Tetramer CMV Assay
Human Multi-Color
372
Tetramer
373
iTAg MHC Tetramer Lyse and
Fixative
Tetramer
374
iTAg Tetramer Class I
Human Tetramer
361
iTAg Tetramer Class II
Human Tetramer
371
iTopia Epitope Discovery System
Customer Package
iTopia
376
iTopia Kit
Tetramer
375
iTopia Start up Kit
iTopia
376
iTopia System Software CD
iTopia
376
IgG
Human Antibody
211
Chicken Antibody
312
Sheep
395
K
Kaluza Sofware
Software
28
Kappa-FITC/Lambda-PE/CD19-ECD
Human Multi-Color
89
KARAP
Human
203
KDR
Human
191
Ki-1 antigen
Human Antibody
126
Ki-67
Human Antibody
221
KIR2DL1
Human Antibody
174
KIR2DL2
Human Antibody
175
KIR2DL3
Human Antibody
175
KIR2DS1
Human Antibody
174
KIR2DS2
Human Antibody
175
467
Alphabetical
KIR2DS4
Human Antibody
176
KIR3DL1
Human Antibody
176
KIR3DS1
Human Antibody
176
KLRF1
Human Antibody
226
KLRG1
Mouse Antibody
291
KOR-SA3544
Human Antibody
222
Kp43
Human Antibody
158
L
L10 Standard
Accessory
23
L3T4a
Mouse Antibody
251
Lactoferrin
Human Antibody
222
LAM-1
Human Antibody
148
Laminin
Mouse Support Reagent
427
Human Antibody
223
LAMP
Human Antibody
183
LAMP-1
Mouse Antibody
276
LAMP3
Human Antibody
149
Langerin
Human Antibody
183
LCA
Human Antibody
138
Mouse Antibody
263
L-CAM
Human Antibody
192
LECAM-1
Human Antibody
148
LECAM-3
Human Antibody
148
Leuko-Trol Platelet Control Kit
Kit
53
Leuko-Trol RBC Control Kit
Kit
53
Leuko64
Human
57
Leukosialin
Human Antibody
136
LeukoSure Enumeration Kit
System
53
Lewis x
Human Antibody
114
Le
x
Human Antibody
114
LFA-1
Human Antibody
109
Mouse Antibody
253
LFA-2
Human Antibody
100
Mouse Antibody
249
LFA-3
Human Antibody
146
LFA- chain
Human Antibody
116
LIFR
Human Antibody
163
LPAM-1
Mouse Antibody
293
LPS Receptor
Human Antibody
113
L-selectin
Human Antibody
148
Mouse Antibody
268
Ly-5
Mouse Antibody
265
Ly-5.1
Mouse Antibody
264
Ly-5.2
Mouse Antibody
264
Ly-6A.2/6E.1
Mouse Antibody
294
Ly-24
Mouse Antibody
262
Ly-51
Mouse Antibody
280
Ly-55
Mouse Antibody
298
Ly-17.1, 17.2
Mouse Antibody
255
Ly-6A/E
Mouse Antibody
294
Ly-6C
Mouse Antibody
294
Ly-6G
Mouse Antibody
295
Ly49C/F/H/I
Mouse Antibody
295
LY49F
Mouse Antibody
296
LY49I
Mouse Antibody
296
Lyb-2.1
Mouse Antibody
270
Lyb-8
Mouse Antibody
257
Lyse and Fixative
Tetramer
374
Lysing Solution
43
Lysing Reagent
Lysing Solution
40
Lysing Solution
44
Lyt-2
Mouse Antibody
253
Lyt-1
Mouse Antibody
252
Light Chain
Rabbit Antibody
320
M
Mac-1
Mouse Antibody
254
Human Antibody
110
MACH
Human Antibody
198
MAdCAM-1
Mouse Antibody
297
Manual CD4 Count Kit
System
49
Marine Iso-Diluent, 10 L
Reagent
58
matrix metalloproteinase-2
Human Antibody
224
Matrix metalloproteinase-3
Human Antibody
224
matrix metalloproteinases-9
Human Antibody
225
MCAM
Chicken Antibody
313
Mch-5
Human Antibody
198
MCP
Human Antibody
141
mdr1 gene product
Human Antibody
185
Mel-CAM
Human Antibody
171
Melanoma cells (membrane ag)
Human Antibody
224
MHC Class I
Chicken Antibody
313
468
Alphabetical
MHC Class II
Mouse Antibody
297
Chicken Antibody
313
MHC II (DQ)
Rabbit Antibody
320
MHC II (DR)
Rabbit Antibody
321
MIRL
Human Antibody
147
MMP-1
Human Antibody
224
MMP-2
Human Antibody
224
MMP-3
Human Antibody
224
MMP-9
Human Antibody
225
MMR
Human Antibody
182
MoFlo XDP
Instrument
19
Monocyte/Granulocyte
Pig Antibody
319
Monocytes/Macrophages
Chicken Antibody
314
Mouse Laminin
Mouse Support Reagent
427
Mouse Serum
Support Reagent
431
Mouse Type IV Collagen
Support Reagent
430
MPL Upgrade
Software
17
MPO
Human Antibody
225
MPO-FITC/Lactoferrin-PE
Human Multi-Color
83
MSP-R
Human Antibody
170
MultiCycle for Windows
Software
17
Myc-Tag
Human Cell Lab
335
Myeloperoxidase
Human Antibody
225
Myeloperoxidase/Lactoferrin
Human Multi-Color
83
MoFlo Astrios
Instrument
21
N
N4 accuvettes
Instrument
23
Navios
Instrument
13
N-CAM
Human Antibody
145
Mouse Antibody
297
Nectin 1
Human Antibody
161
Nectin 2
Human Antibody
161
Neg./Neg./CD45
Human Multi-Color
92
Neg./Neg./Neg.
Human Multi-Color
73
Neg.Ctrl.-FITC/Neg.Ctrl.-PE/CD19-
ECD
Human Multi-Color
90
ECD
Human Multi-Color
90
ECD
Human Multi-Color
90
Neg.Ctrl.-FITC/Neg.Ctrl.-PE/Neg.
Ctrl.-ECD
Human Multi-Color
90
Negative Tetramers
Human Tetramer
361
Neu
Human Antibody
198
Nitroblue tetrazolium
Support Reagent
428
NBT
Support Reagent
428
NK1.1
Mouse Antibody
298
NKG2-A/C/E
Mouse Antibody
298
NKG2a
Human Antibody
177
Human Antibody
158
NKG2D
Human Antibody
191
NKH-1
Human Antibody
145
NKp30
Human Antibody
193
NKp44
Human Antibody
193
NKp46
Human Antibody
192
NKp80
Human Antibody
226
NKR-P1
Human Antibody
178
NTB-A
Human Antibody
226
Nuclear Antigen Ki-67
Human Antibody
221
Nuclear Isolation and Stain (NIM-
DAPI 10), 250 mL
Reagent
58
Nuclear Isolation Media (NIM), 100
mL
Reagent
58
O
Offline Analysis Workstation
Instrument
17
o-nitrophenyl-b-D-galatopyranoside
substrate
Support Reagent
428
ONGP
Support Reagent
428
Optilyse B
Lysing Solution
42
OptiLyse C
Lysing Solution
42
OptiLyse No-Wash Lysing Solutions
Lysing Solution
42
Optima
Instrument
27
Optima L-XP Series
Instrument
27
OSCAR
Human Antibody
227
OX 40
Human Antibody
169
P
p50.3
Human Antibody
176
p53 Protein
Human Antibody
227
p75 AIRM1
Human Antibody
228
P-glycoprotein
Human Antibody
185
p-MAPKAPK-2 (Thr334)
Cell Lab
345
p-nitrophenyl phosphate substrate
Support Reagent
429
p-NPP
Support Reagent
429
p-RPS6
Cell Lab
349
469
Alphabetical
P-selectin
Human Antibody
148
p-Stat3 (Tyr 705)
Cell Lab
352
PADGEM
Human Antibody
148
Pan B cells
Human Antibody
140
Pan Keratin
Human Cell Lab
336
Pan NK
Mouse Antibody
266
PAP pen
Support Reagent
431
PAS IV
Human Antibody
131
PBS Buffer
Support Reagent
427
PC7 (770/488) Set-up Kit
Fluorosphere
36
PC7 Filter Kit for EPICS XL
Kit
50
PCNA
Human Antibody
229
PD-1
Human
189
PD-L1
Human Antibody
188
PD-L2
Human Antibody
187
PDCD1LG1
Human Antibody
188
PDCD1LG2
Human Antibody
187
PECAM-1
Human Antibody
126
Mouse Antibody
261
PEN5
Human Antibody
178
PerFix-nc
Lysing Solution
42
PerFix-p
Lysing Solution
43
Perforin
Human Antibody
228
Permeabilization Reagent
Lysing Solution
40
Peroxidase
Detection Kit
426
Pgp-1
Human Antibody
136
Mouse Antibody
262
Phospho-4E-BP1
Human Cell Lab
337
Phospho-Akt (Ser473)
Human Cell Lab
338
Phospho-Akt (Thr308)
Human Cell Lab
339
Phospho-Bcl-2
Human Cell Lab
340
Phospho-CREB (Ser133)
Human Cell Lab
341
phospho-Erk1/2 (T202/Y204)
Cell Lab
342
phospho-H3 Histone (Ser10)
Cell Lab
344
Phospho-H2A.X Histone
Cell Lab
343
phospho-MAPKAPK-2 (Thr334)
Cell Lab
345
phospho-NF-kB p65 (Ser536)
Cell Lab
346
phospho-p38 MAPK (T180/Y182)
Cell Lab
347
phospho-RPS6
Cell Lab
349
phospho-SAPK/JNK (T183/Y185)
Cell Lab
350
phospho-Stat3 (Tyr 705)
Cell Lab
352
phospho-Tyrosine
Cell Lab
353
Phospho-p53 (Ser15)
Human Cell Lab
348
Phospho-Stat1 (Tyr701)
Cell Lab
351
Phosphotyrosine
Human Antibody
228
PKB
Cell Lab
Human Antibody
194
Platelet Stabilizer
Lysing Solution
43
Polio Virus Receptor
Human Antibody
173
PRAD1
Human Antibody
201
PrepPlus 2 Accessory Kit
Cell Preparation
30
Cell Preparation
30
Progesterone Receptor
Human Antibody
229
Proliferation Cell Nuclear Antigen
Human Antibody
229
Protein A
Streptavidin
423
Protein G
Streptavidin
423
Protein L
Streptavidin
423
PRR1
Human Antibody
161
PRR2
Human Antibody
161
PSGL-1
Human Antibody
178
PU.1
Human Cell Lab
354
PuraFlow Sheath Fluid
Instrument
59
Q
Qa-2
Mouse Antibody
299
QuickCOMP 2 Kit
Quality Control
38
QuickCOMP 4 Kit
Human Quality Control
38
R
Rabbit Anti-Goat IgG (H+L)
Goat Secondary
398
Rabbit Anti-Hamster IgG (H+L)
Hamster Secondary
400
Rabbit Anti-Human IgG (H+L)
Human Secondary
405
Rabbit Anti-Mouse IgG (H+L)
Mouse Secondary
414
Rabbit Anti-Rat IgG (H+L)
Rat Secondary
421
Rabbit Anti-Sheep IgG (H+L)
Sheep Secondary
422
Rabbit F(ab)
2
Anti-Goat IgG (H+L)
Goat Secondary
398
Rabbit F(ab)2 Anti-Human IgG (H
+L)
Human Secondary
406
Rabbit F(ab)2 Anti-Mouse IgG (H+L)
Mouse Secondary
415
Rabbit F(ab)2 Anti-Rat IgG (H+L)
Rat Secondary
421
Rabbit F(ab)2 Anti-Sheep IgG (H+L)
Sheep Secondary
422
Rabbit Serum
Support Reagent
431
Rat Type I Collagen
Support Reagent
430
470
Alphabetical
Red Diode Laser Upgrade
Instrument
17
Residual Leukocyte Enumeration Kit
System
53
Resin-based Mounting Medium
Support Reagent
431
reticONE Reagent Kit
Instrument
49
Software
49
Rinsol Cleaning Agent
Instrument
60
RON
Human Antibody
170
Rotor for Optima
Instrument
27
RT1B
Mouse Antibody
297
S
S-Endo
Human Antibody
171
S-Tag
Human
424
Sample Tubes
Instrument
60
Sca-1
Mouse Antibody
294
SCF receptor
Human Antibody
162
Second Mitochondria-derived
Activator Of Caspase
Human Antibody
229
Sheep Anti-FITC
Secondary
396
Sheep red blood cell rosettes
Human Antibody
100
Shutdown Solution, 5 L
Reagent
58
Slide mounting medium
Support Reagent
431
SMAC
Human Antibody
229
Solastra B Lineage Kit
Human System
55
Solastra Myelomonocytic Lineage Kit
Human System
55
Solastra T Lineage Kit
Human System
55
Solution Set for Streptamer Magnetic
Beads
Streptamer
378
stem cell-derived tyrosine kinase
Human Antibody
170
Stem-Kit Reagents
System
47
Quality Control
38
Stem-Comp Reagent
System
39
Software
17
Software
48
STK
Human Antibody
170
Strep-Tactin
Streptamer
377
Streptamer Magnetic Beads
Streptamer
378
Streptavidin
Streptavidin
424
Stromelysin-1
Human Antibody
224
substrate ABTS
Support Reagent
428
Super PAP Pen
Support Reagent
431
Survivin
Human Cell Lab
355
SW 32 Ti
Instrument
27
SWC3a
Pig Antibody
319
Swine Anti-Goat IgG (H+L)
Goat Secondary
399
Syndecan-1
Human Antibody
170
T
T10 antigen
Human Antibody
132
TAC
Human Antibody
123
TAPA-1
Human Antibody
153
Mouse Antibody
271
TCR /
Mouse Antibody
299
TCR /
Mouse Antibody
299
Chicken Antibody
314
TCR PAN /
Human Antibody
230
TCR PAN /
Human Antibody
230
TCR V24
Human Antibody
230
TCR V1
Human Antibody
231
Chicken Antibody
314
TCR V2
Human Antibody
231
Chicken Antibody
315
TCR V3
Human Antibody
231
TCR V4
Human Antibody
232
TCR V5.1
Human Antibody
232
TCR V5.2
Human Antibody
232
TCR V5.3
Human Antibody
233
TCR V7.1
Human Antibody
233
TCR V7.2
Human Antibody
233
TCR V8
Human Antibody
234
TCR V9
Human Antibody
234
TCR V11
Human Antibody
234
TCR V12
Human Antibody
235
TCR V13.1
Human Antibody
235
TCR V13.2
Human Antibody
235
TCR V13.6
Human Antibody
236
TCR V14
Human Antibody
236
TCR V16
Human Antibody
236
TCR V17
Human Antibody
237
TCR V18
Human Antibody
237
TCR V20
Human Antibody
237
TCR V21.3
Human Antibody
238
TCR V22
Human Antibody
238
TCR V23
Human Antibody
238
471
Alphabetical
TCR V Repertoire Kit
Human System
54
TCR V1
Human Antibody
239
TCR V2
Human Antibody
239
TCR V9
Human Antibody
239
TCR
Human Antibody
240
TdT
Human Antibody
240
TdT Monoclonal Kit
System
53
TdT Polyclonal Kit
System
53
Software
17
Software
46
Thrombin receptor
Human Antibody
241
ThromboFix Platelet Stabilizer
Lysing Solution
43
Thrombospondin
Human Antibody
241
Thy-1
Human Antibody
157
Mouse Antibody
272
Thy-1.2
Mouse Antibody
273
Thyroglobulin
Human Antibody
242
TIA-1
Human Antibody
242
TMB substrate solution
Support Reagent
429
TNF
Human Antibody
243
Mouse Antibody
300
TNF-R2
Human Antibody
164
Tp44
Human Antibody
124
TQ-Prep Workstation
Cell Preparation
29
Transferrin receptor
Human Antibody
151
Transin
Human Antibody
224
TRAP
Human Antibody
172
triCHROME
Human Multi-Color
72
Tumor necrosis factor-receptor type 2
Human Antibody
164
Type 70.1 Ti
Accessory
27
Type IV collagenase
Human Antibody
224
TYROBP
Human
203
U
Ultracentrifuges
Instrument
27
UltraTech-AEC
Detection Kit
426
UltraTech-DAB
Detection Kit
426
UltraTech-HRP-AEC
Detection Kit
425
UltraTech-HRP-DAB
Detection Kit
425
UltraTech-Kits
Detection Kit
426
V
VCAM-1
Human Antibody
160
Mouse Antibody
275
VE-Cadherin
Human Antibody
171
VEA
Mouse Antibody
269
VersaLyse Lysing Solution
Lysing Solution
43
Vi-CELL XR Quad Pack
Instrument
22
Vi-CELL Concentration Control
Instrument
22
Vi-Cell Focus Control
Instrument
22
Vi-CELL Sample Cups
Accessory
22
Vi-CELL XR Single Pack
Instrument
22
Vi-CELL XR
Instrument
22
Villin
Human Antibody
243
Vimentin
Human Antibody
244
VLA-2
Human Antibody
141
Mouse Antibody
266
VLA-3
Human Antibody
142
VLA-4
Human Antibody
142
Mouse Antibody
267
VLA-5
Human Antibody
142
Mouse Antibody
267
VLA-6
Human Antibody
143
VLA chain
Human Antibody
125
von Willebrand's factor
Human Antibody
244
W
Whole Blood Lysing Reagents
Lysing Solution
44
Z
Z-Series
Instrument
23
Z-Series Tri-Pak
Accessory
23
Z1 Dual Threshold Analyzer
Instrument
23
Z1 Single Threshold Analyzer
Instrument
23
Z2 AccuComp Software
Software
23
Z2 Analyzer
Instrument
23
ZAP-70
Cell Lab
356
-Feto Protein
Human Antibody
195
-catenin
Human Cell Lab
326
4 integrin
Mouse Antibody
275
Mark TCR V Repertoire Kit
Human System
54
2-microglobulin
Human Antibody
197
Chicken Antibody
309
473
Alphabetical
Chain
Human Antibody
221
Mouse Antibody
292
Rat Antibody
303
Chain
Human Antibody
223
Mouse Antibody
293
Chicken Antibody
312
473
Australia, Gladesville (61) 2 9844-6000 Austria, Vienna (43) 1 291 01 241 Canada, Mississauga (1) 905 819 1234
China, Shanghai (86) 21 3865 1000 Croatia, Zagreb (38) 51 489 9003 Czech Republic, Prague (420) 272 01 73 32
Eastern Europe, Middle East, North Africa, South West Asia: Switzerland, Nyon (41) 22 365 3707 France, Villepinte (33) 1 49 90 90 00
Germany, Krefeld (49) 2151 33 35 Hong Kong (852) 2814 7431 India, Mumbai (91) 22 3080 5000 Italy, Cassina de Pecchi, Milan (39) 02 953921
Japan, Tokyo (81) 3 5530 8500 Korea, Seoul (82) 2 404 2146 Latin America (1) (305) 380 4709 Malaysia, Kuala Lumpur (60) 3 56214793
Mexico, Mexico City (001) 52 55 9183 2800 Netherlands, Woerden (31) 348 462462 Poland, Warszawa (48) 223660180Puerto Rico (787) 747 3335
Singapore (65) 6339 3633 South Africa/Sub-Saharan Africa, Johannesburg (27)11564 3203 Spain, Madrid (34) 91 3836080
Sweden, Bromma (46) 8 564 85 900 Switzerland, Nyon (41) 0800 850 810 Taiwan, Taipei (886) 2 2730 2500 Turkey, Istanbul (90) 216 570 17 17
UK, High Wycombe (44) 01494 441181 USA, Brea, CA (1) 800 742 2345
B2009-10605 www.beckmancoulter.com 2010 Beckman Coulter, Inc.
June 2010

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B2011-12691

T suppressor (Ts) cells may

NK cell population, 80% of these cells being CD56

cells in the immune system and

cells. The magnitude of lysis correlates with its level of surface

subset. With NKp46 and NKp44 it is one of the three identified NK

T cells, reactive to bacterial antigens. This unique lymphocyte population restricted by

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