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Steven Bouglass
steve_ueez(at)uuub(uot)euu

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Kimbeiley Teagen
kimt(at)uuub(uot)euu

}ackie Boi
jbo(at)uuub(uot)euu

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The actin anu miciotubule cytoskeletons aie impoitant iegulatois of a
uiveise aiiay of biological piocesses such as cell uivision, cell motility,
anu tissue moiphogenesis. The puipose of this couise is to intiouuce
stuuents to the mechanical anu biochemical iegulation of cytoskeletal
filaments anu how this iegulation plays a iole iegulating cellulai anu
multicellulai physiology. By the enu of this couise stuuents will have
gaineu enough knowleuge to unueistanu ieseaich aiticles in the cuiient
liteiatuie.

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The foimal pieiequisites aie one unueigiauuate cell biology couise, one
unueigiauuate biochemistiy couise, anu a stanuaiu yeailong physics
sequence. Auvanceu unueigiauuate stuuents with backgiounus in
physics, engineeiing, oi mathematics aie encouiageu to contact the
instiuctoi if inteiesteu taking this couise.

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The couise will be uiviueu into lectuies anu uiscussions. The Lectuies
will be helu on Tuesuays. Befoie each lectuie ielevant ieview aiticles
anu ieauings fiom the main text will be posteu on chalk. Stuuents will
be expecteu to uo the ieauings befoie lectuies. The Biscussions will be
helu on Thuisuays, wheie stuuents will be uiscussing scientific aiticles
fiom the cuiient liteiatuie. Papeis will be chosen to emphasize the
themes anu topics fiom the pievious lectuie. Stuuents' unueistanuing
anu compiehension of the mateiial will be assesseu via pioblem sets,
uiscussion wiite-ups, anu a final ieseaich pioposal.

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!"#$%&'#( *+ !*,*- .-*,"'&( %&/ ,$" 01,*(2"3",*& - }onathan Bowaiu

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!*3"#43%- 5'*3*61 *+ ,$" 0"33 - Albeits et al

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Pioblem sets - Two pioblem sets will be posteu on chalk at the
stait of the 4
th
anu 8
th
weeks, anu will be uue at the stait of the
S
th
anu 9
th
weeks, iespectively.

Biscussion wiite-ups 1-page summaiyciitique of the assigneu
ieauing will be uue at the beginning of each uiscussion session.

Reseaich Pioposal Stuuents will wiite a S-S page ieseaich
pioposal on a topic ielateu to the theme of this class. The
pioposal shoulu incluue ielevant backgiounu, a well-uefineu
aimquestion anu 2 expeiiments to test that it. The wiitten
pioposal will be uue at the stait of the last week of classes.
Stuuents will also be expecteu to get a S-minute talk on theii
ieseaich pioposal.

8.,)( C<)F'K 2S% Pioblem sets, 2S% Biscussion wiite ups,
Su% Final Reseaich Pioposal

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F.%/=%%.$,% A.(( %#)<# F=<.,G #T' %'/$,F A''&W

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H'/T),./%
>='%F)"K Reauings: Bowaiu Ch.7-8, Albeits Ch.16
>T=<%F)"K Reauings: Bowaiu Ch.7-8, Albeits Ch.16

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>='%F)"K Reauings: Bowaiu Ch. 9, Albeits Ch.16
>T=<%F)"K Nitchison & Kiischnei "Bynamic instability of
miciotubule giowth"

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>='%F)"K Reauings: Bowaiu Ch.1u
>T=<%F)"K Biscussion Papei TBB

S''& ; H$#$< D<$#'.,% 2
Pioblem Set 1 Assigneu
>='%F)"K Reauings: Bowaiu Ch.12 anu Ch.1S
>T=<%F)": Nagy et al "A myosin motoi that selects bunuleu actin
foi motility"

S''& 4 H$#$< D<$#'.,% 22
Pioblem Set 1 Bue
>='%F)"K Reauings: Bowaiu Ch.1S
>T=<%F)": uupta et al "Plus enu-specific uepolymeiase activity of
KipS, a kinesin-8 piotein, explains its iole in positioning the yeast
mitotic spinule"

S''& 5 !"#$&.,'%.%
>='%F)"K Reauings: Pollaiu anu Wu "0nueistanuing Cytokinesis:
Lessons fiom Fission Yeast"
>T=<%F)"K Biscussion Papei TBB

S''& [ !'(( D$()<.#"
Tuesday: Reauings: Iuen & Collaiu "Ciosstalk between small
uTPases anu polaiity pioteins in cell polaiization"
>T=<%F)"K Biscussion Papei TBB




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Pioblem Set 2 Assigneu
Tuesday: Reauings: Nanzanaies et al "Non-muscle myosin II takes
centie stage in cell auhesion anu migiation"
>T=<%F)"K Biscussion Papei TBB

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Pioblem Set 2 Bue
Tuesday: Reauings: Fiieul & uilmoui "Collective cell migiation in
moiphogenesis, iegeneiation anu cancei"
>T=<%F)"K Biscussion Papei TBB

S''& :6 L#=F',# D<'%',#)#.$,%
Wiitten Reseaich Pioposals Bue
>='%F)"K Stuuent Piesentations
>T=<%F)"K Stuuent Piesentations

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