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01/08/2010
Linear DNA = multiple replication sites
Many OriRep – about 40,000 bases apart
Replicated in 15-30 minutes
Replication of full genome (many chromosomes) = 5-10 hours
Okazaki fragments: 1000-2000bp in Prok, 100-200bp in Eukaryotic
Important Replication Proteins
Gyrase – topoisomerase II (double-stranded break)
Helicase – Unwinds DNA at replication fork to separate parental
strands
SSB – stabilizes single-stranded DNA
Primosome – creates RNA primer
o RNA Primase
DNA Polymerase – 5’->3’ Polymerization + proofreading functions
In Euk, RNA primer removed by RPA (Replication Protein A)
In Prok, RNA primer removal/replacement by DNA Pol I.
Both use DNA ligase
Yy yy