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Table Of Contents

GENERAL INSTRUCTION
GENERAL LABORATORY METHODS
SPECTROPHOTOMETRY
COLUMN CHROMATOGRAPHY
pH METER
CENTRIFUGATION
ULTRASONIC CELL DISRUPTION
CONDUCTIVITY METER
RADIOACTIVE TRACERS
AUTORADIOGRAPHY
PHOTOGRAPHY
STATISTICS
GRAPHS
COMPUTERS
CARBOHYDRATES
EXERCISE 1. QUALITATIVE TESTS
EXERCISE 2. QUALITATIVE ANALYSIS
EXERCISE 3. ESTIMATE THE AMOUNT OF REDUCING SUGARS
EXERCISE 4. ESTIMATION OF REDUCING SUGAR BY SOMOGYI’S METHOD
EXERCISE 5. ESTIMATION OF SUGAR BY FOLIN-WU METHOD
EXERCISE 6. ESTIMATION OF SUGAR BY HAGEDORN-JENSON METHOD
EXERCISE 11. ESTIMATION OF PROTEIN BY THE BIURET METHOD
EXERCISE 12.ESTIMATION OF PROTEIN BY THE FC-METHOD
EXERCISE 13. PROTEIN ASSAY BY BRADFORD METHOD
EXERCISE 14.ESTIMATION OF PROTEIN BY THE LOWRY PROTEIN ASSAY
EXERCISE 15. BIURET PROTEIN ASSAY
EXERCISE 16.ESTIMATION OF DNA BY THE DIPHENYLAMINE METHOD
EXERCISE 17. ESTIMATION OF RNA BY THE ORCINOL METHOD
ENZYMES
EXERCISE 1.DEMONSTRATING THE PRESENCE OF CATALASE IN PIG’S LIVER
EXERCISE 2. DETERMINING THE OPTIMUM pH FOR TRYPSIN
EXERCISE 3. EXTRACTION OF TYROSINASE
EXERCISE 4. PREPARATION OF STANDARD CURVE
EXERCISE 5. ENZYME CONCENTRATION
EXERCISE 6. EFFECTS OF pH
EXERCISE 7. EFFECTS OF TEMPERATURE
EXERCISE 8. COMPUTER SIMULATION OF ENZYME ACTIVITY
EXERCISE 9. KINETIC ANALYSIS
EXERCISE 11. ADDITION OF ENZYME INHIBITORS
EXERCISE 12. PROTEIN CONCENTRATION/ENZYME ACTIVITY
EXERCISE 16.CONSTRUCTION OF THE MALTOSE CALIBRATION CURVE
ELECTROPHORESIS
EXERCISE 1. PREPARATION OF SDS-POLYACRYLAMIDE GELS
EXERCISE 2. SEPARATION OF PROTEIN STANDARDS: SDS-PAGE
EXERCISE 3. COOMASSIE BLUE STAINING OF PROTEIN GELS
EXERCISE 4. SILVER STAINING OF GELS
EXERCISE 5. DOCUMENTATION
EXERCISE 6. WESTERN BLOTS
PREPARATION OF POLYACRYLAMIDE GELS
AGAROSE GEL ELECTROPHORESIS
INTRODUCTION
THE MICROSCOPY
EXERCISE 1. THE BRIGHT FIELD MICROSCOPE
Stage micrometer
EXERCISE 4. MEASURING DEPTH
EXERCISE 5. MEASURING AREA
EXERCISE 6.CELL COUNT BY HEMOCYTOMETER OR MEASURING VOLUME
EXERCISE 7. MEASUREMENT OF CELL ORGANELLES
EXERCISE 8. USE OF DARKFIELD ILLUMINATION
EXERCISE 9. THE PHASE CONTRAST MICROSCOPE
EXERCISE 10. THE INVERTED PHASE MICROSCOPE
ASEPTIC TECHNIQUE AND TRANSFER OF MICROORGANISMS
CONTROL OF MICROORGANISMS BY USING PHYSICAL AGENTS
CONTROL OF MICROORGANISMS BY USING ANTIMICROBIAL CHEMOTHERAPY
ISOLATION OF PURE CULTURES FROM A MIXED POPULATION
BACTERIAL STAINING
DIRECT STAIN AND INDIRECT STAIN
GRAM STAIN AND CAPSULE STAIN
ENDOSPORE STAINING AND BACTERIAL MOTILITY
ENUMERATION OF MICROORGANISMS
BIOCHEMICAL TEST FOR IDENTIFICATION OF BACTERIA
TRIPLE SUGAR IRON TEST
STARCH HYDROLYSIS TEST (II METHOD)
EXTRACTION OF BACTERIAL DNA
MEDICALLY SIGNIFICANT GRAM–POSITIVE COCCI (GPC)
PROTOZOANS, FUNGI, AND ANIMAL PARASITES
THE FUNGI, PART 1–THE YEASTS
PERFORMANCE OBJECTIVES
THE FUNGI, PART 2—THE MOLDS
VIRUSES: THE BACTERIOPHAGES
SEROLOGY, PART 1–DIRECT SEROLOGIC TESTING
SEROLOGY PART 2—INDIRECT SEROLOGIC TESTING
CELL CYCLES
MEIOSIS IN FLOWER BUDS OF ALLIUM CEPA-ACETOCARMINE STAIN
MEIOSIS IN GRASSHOPPER TESTIS (POECILOCERUS PICTUS)
MITOSIS IN ONION ROOT TIP (ALLIUM CEPA)
DIFFERENTIAL STAINING OF BLOOD
BUCCAL EPITHELIAL SMEAR AND BARR BODY
VITAL STAINING OF DNA AND RNA IN PARAMECIUM
INDUCTION OF POLYPLOIDY
MOUNTING OF GENITALIA IN DROSOPHILA MELANOGASTER
MOUNTING OF GENITALIA IN THE SILK MOTH BOMBYX MORI
MOUNTING OF THE SEX COMB IN DROSOPHILA MELANOGASTER
MOUNTING OF THE MOUTH PARTS OF THE MOSQUITO
NORMAL HUMAN KARYOTYPING
KARYOTYPING
STUDY OF DRUMSTICKS IN THE NEUTROPHILS OF FEMALES
Study of drumsticks in neutrophils of females
STUDY OF THE MALARIA PARASITE
SEX-LINKED INHERITANCE IN DROSOPHILA MELANOGASTER
PREPARATION OF SOMATIC CHROMOSOMES FROM RAT BONE MARROW
CHROMOSOMAL ABERRATIONS
STUDY OF PHENOCOPY
STUDY OF MENDELIAN TRAITS
ESTIMATION OF NUMBER OF ERYTHROCYTES [RBC] IN HUMAN BLOOD
ESTIMATION OF NUMBER OF LEUCOCYTES (WBC) IN HUMAN BLOOD
CULTURING TECHNIQUES AND HANDLING OF FLIES
LIFE CYCLE OF THE MOSQUITO (CULEX PIPIENS)
LIFE CYCLE OF THE SILKWORM (BOMBYX MORI)
VITAL STAINING OF EARTHWORM OVARY
CULTURING AND OBSERVATION OF PARAMECIUM
CULTURING AND STAINING OF E.COLI (GRAM’S STAINING)
BREEDING EXPERIMENTS IN DROSOPHILA MELANOGASTER
PREPARATION OF SALIVARY GLAND CHROMOSOMES
OBSERVATION OF MUTANTS IN DROSOPHILA MELANOGASTER
ABO BLOOD GROUPING AND Rh FACTOR IN HUMANS
DETERMINATION OF BLOOD GROUP AND Rh FACTOR
DEMONSTRATION OF THE LAW OF INDEPENDENT ASSORTMENT
DEMONSTRATION OF LAW OF SEGREGATION
THE CENTRAL DOGMA
EXERCISE 1. PROTEIN SYNTHESIS IN CELL FREE SYSTEMS
CHROMOSOMES
EXERCISE 2. POLYTENE CHROMOSOMES OF DIPTERANS
EXERCISE 3. SALIVARY GLAND PREPARATION (SQUASH TECHNIQUE)
EXERCISE 4. EXTRACTION OF CHROMATIN
EXERCISE 5. CHROMATIN ELECTROPHORESIS
EXERCISE 6. EXTRACTION AND ELECTROPHORESIS OF HISTONES
EXERCISE 7. KARYOTYPE ANALYSIS
EXERCISE 8. IN SITU HYBRIDIZATION
EXERCISE 9. CULTURING PERIPHERAL BLOOD LYMPHOCYTES
EXERCISE 11. STAINING CHROMOSOMES (G-BANDING)
NUCLEIC ACIDS
EXERCISE 12. EXTRACTION OF DNA FROM BOVINE SPLEEN
EXERCISE 13. PURIFICATION OF DNA
EXERCISE 14. CHARACTERIZATION OF DNA
EXERCISE 15. DNA-DISCHE DIPHENYLAMINE DETERMINATION
EXERCISE 16. MELTING POINT DETERMINATION
EXERCISE 17. CsCl-DENSITY SEPARATION OF DNA
EXERCISE 18. PHENOL EXTRACTION OF rRNA (RAT LIVER)
EXERCISE 19. SPECTROPHOTOMETRIC ANALYSIS OF rRNA
EXERCISE 21. SUCROSE DENSITY FRACTIONATION
EXERCISE 22. NUCLEOTIDE COMPOSITION OF RNA
ISOLATION OF GENOMIC DNA—DNA EXTRACTION PROCEDURE
EXERCISE 23. ISOLATION OF GENOMIC DNA FROM BACTERIAL CELLS
EXERCISE 24. PREPARATION OF GENOMIC DNA FROM BACTERIA
EXERCISE 25. EXTRACTION OF GENOMIC DNA FROM PLANT SOURCE
EXERCISE 26. EXTRACTION OF DNA FROM GOAT LIVER
EXERCISE 35. ISOLATION OF PLASMIDS
EXERCISE 36. RNA ISOLATION
EXERCISE 37. RNA EXTRACTION METHOD FOR COTTON
EXERCISE 38. ISOLATION OF RNA FROM BACTEROIDS
EXERCISE 39. ISOLATION OF RNA FROM FREE-LIVING RHIZOBIA
ESTIMATION OF DNA PURITY AND QUANTIFICATION
EXERCISE 40. FUNGAL DNA ISOLATION
EXERCISE 41. METHYLENE BLUE DNA STAINING
EXERCISE 42. TRANSFORMATION
BLOTTING TECHNIQUES—SOUTHERN, NORTHERN, WESTERN BLOTTING
PREPARING THE PROBE
EXERCISE 43. SOUTHERN BLOTTING (FIRST METHOD)
EXERCISE 44. SOUTHERN BLOTTING (SECOND METHOD)
EXERCISE 45. WESTERN BLOTTING
SOUTHERN BLOT
EXERCISE 47. SOUTHERN ANALYSIS OF MOUSE TOE/TAIL DNA
EXERCISE 48. NORTHERN BLOTTING
RESTRICTION DIGESTION METHODS—RESTRICTION ENZYME DIGESTS
EXERCISE 52. RESTRICTION ENZYME DIGESTION OF DNA
EXERCISE 55. LIGATION OF INSERT DNA TO VECTOR DNA
PCR METHODS (POLYMERASE CHAIN REACTION)
EXERCISE 56. POLYMERASE CHAIN REACTION
EXERCISE 57. DNA AMPLIFICATION BY THE PCR METHOD
TISSUE CULTURE METHODS
PLANT TISSUE CULTURE
MANY DIMENSIONS OF PLANT TISSUE CULTURE RESEARCH
WHAT IS PLANT TISSUE CULTURE?
USES OF PLANT TISSUE CULTURE
DEMONSTRATION OF TISSUE CULTURE FOR TEACHING
PREPARATION OF PLANT TISSUE CULTURE MEDIA
PLANT TISSUE CULTURE MEDIA
PREPARATION OF PROTOPLASTS
PROTOPLAST ISOLATION, CULTURE, AND FUSION
ISOLATION OF CHLOROPLASTS FROM SPINACH LEAVES
PREPARATION OF PLANT DNA USING CTAB
SUSPENSION CULTURE AND PRODUCTION OF SECONDARY METABOLITES
PROTOCOLS FOR PLANT TISSUE CULTURE
STERILE METHODS IN PLANT TISSUE CULTURE
MEDIA FOR PLANT TISSUE CULTURE
SAFETY IN PLANT TISSUE CULTURE
PREPARATION OF MEDIA FOR ANIMAL CELL CULTURE
ASEPTIC TECHNIQUE
CULTURE AND MAINTENANCE OF CELL LINES
TRYPSINIZING AND SUBCULTURING CELLS FROM A MONOLAYER
CELLULAR BIOLOGY TECHNIQUES
IN VITRO METHODS
HUMAN CELL CULTURE METHODS
STARCH HYDROLYSIS TEST
GELATIN HYDROLYSIS TEST
CATALASE TEST
OXIDASE TEST
CULTURE EXPERIMENTS
INDEX
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Biotechnology Procedures and Experiments Handbook

Biotechnology Procedures and Experiments Handbook

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Published by: sampangpu on Oct 17, 2011
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