Physical Agents of Control – Electromagnetic Radiation
To study the effect of ultraviolet (UV) radiation on microorganism
Certain forms of electromagnetic radiation are capable of producing a lethal effect on cellsand therefore can be used for microbial control. Electromagnetic radiations that possesssufficient energy to be microbicidal are the short-wavelength radiation, that is, below300nm. These include UV, gamma rays, and x-rays. The high wavelength radiations, thoseabove 300nm, have insufficient energy to destroy cells.
Materials, Culture, and Media
24 to 48 hours nutrient broth cultures of
; inoculating loop, ultraviolet radiationsource, parafilm, 6 nutrient agar plates for each group.
All plates were inoculated in a sterile manner, by means of streak inoculation.2.Cover of each inoculated plate was labeled according to the exposure time toultraviolet radiation: 0 (control), 15 seconds, 30 seconds, 1 minute, 3 minutes, 5minutes, and 5 minutes (irradiated covered control)
All inoculated plates were irradiated for the designated period of time by placed at12 inches below the ultraviolet light sources. All petri dish covers was removedexcept that of the 5-minutes irradiated control plate.
All plates were incubated in an inverted position for 4 to 5 days at 30°C.
1.All nutrient agar plate cultures were observed for the amount of growth of microbe.
Observation was recorded as: 0 = no growth; 1+ = slight growth; 2+ = moderategrowth; 3+ = abundant growth.