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Published by Azreen Anis

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Published by: Azreen Anis on Apr 10, 2012
Copyright:Attribution Non-commercial


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Objective : To determine Cadmium (Cd) and Chromiun (Cr) in plant tissue using absorptionspectroscopyIntroductions :Cadmium is well known to be one of the most toxic heavy elements for animals. It has recentlybecome a serious problem that rice grains contain cadmium in some area of Japan. On the otherhand, some types of plants can grow in contaminated soils and absorb a large amount of cadmium in their bodies. Such hyperaccumulator plants are expected to be used for remediationof environments. However, the accumulation mechanism has not yet been revealed, with theelemental distribution of cadmium and transportation during uptake remaining unclear.Cadmium appear to play a very pivotal role in thyroiddisease,it is a very unique mineral. It isextremely toxic and has toxic biological effects at concentrations smaller than almost anycommonly found mineral. An environmental poison found in water, on our food and in the air.It's found in processed grains, dairy products, meats, fish, fertilizers, auto exhaust, cigarettesmoke, batteries, solder and dentures. It disrupts the absorption of other minerals and tends tosettle in the heart and right kidney and affects proper functioning of severalenzymes. Whereas for Chromium, taken in the right quantity, chromium has immense health benefits. It isavailable in extremely low quantities in animal and plant tissues which is why it is called a trace
metal. Some of the sources of chromium are brewer’s yeast, coffee, tea, cereals, potato
es, peas,oysters, rye, thyme, processed meats, whole grains, and beer.Chromium helps metabolize carbohydrates. It monitors blood sugar levels, and helps stabilizeblood sugar. It can also prevent hypertension or high blood pressure. Although trials are stillbeing conducted, chromium compounds are considered helpful in preventing memory loss and in
treating Alzheimer’s disease.
 Procedure : Day 11. Plant tissue was prepared ( mustard and spinach).
2. The vegetable was dried in and oven of 110’C
 Day 21. The dried vegetables was cut into pieces2. About 3 grams of vegetable was weighed and placed in 250 ml beaker3. 10 ml of nitric acid (HNO
) was added into the beaker and was allowed to standOvernight
Day 3A) Sample preparation1. The sample was heated until red fume came out2. Then it was cooled3. 1 ml of peroxide (H
) was added into the cooled sample4.The sample was reheated till concentrate5. It was then filtered into 250ml volumetric flask 6. The sample was diluted with distilled water till markedB) Standard preparationCr Volume Cd Volume1 ppm 0.5 ml 0.2 ppm 1 ml2 ppm 1.0 ml 0.4 ppm 2 ml3 ppm 1.5 ml 0.6 ppm 3 ml4 ppm 2.0 ml 0.8 ppm 4 ml5 ppm 2.5 ml 1.0 ppm 5 ml1. The standard was prepared as in table given above in 100 ml volumetric flask 2. The stock solution given was in 1000 ppm for each sample3. 5 ml of the stock solution was pipette into a 100 ml volumetric flask and diluted withdistilled water till marked4. For each concentration, the volume was pipette into a 50 volumetric flask as stated inthe table above respectively5. 7 ml of the sample was pipette into each volumetric flask and was diluted with distiiledwater till mark 6. The sample was then analyzed using atomic absorption spectroscopy
Results :CONCENTRATION ABSORBANCE VOLUMECHROMIUM CADMIUM CHROMIUM CADMIUM CHROMIUM CADMIUMBlank Blank 0.036 0.264 0 01 ppm 0.2 ppm 0.045 0.290 0.5 ml 1 ml2 ppm 0.4 ppm 0.058 0.311 1.0 ml 2 ml3 ppm 0.6 ppm 0.071 0.330 1.5 ml 3 ml4 ppm 0.8 ppm 0.082 0.352 2.0 ml 4 ml5 ppm 1.0 ppm 0.093 0.369 2.5 ml 5 mlCalculation :Least square method :CONCENTRATIONABSORBANCE(y
) X
Cr Cd Cr Cd Cr Cd Cr Cd Cr CdBlank Blank 0.036 0.264 0 0 0 0 0 01 ppm 0.2 ppm 0.045 0.290 0.5 ml 1 ml 0.25 1 0.0225 0.2902 ppm 0.4 ppm 0.058 0.311 1.0 ml 2 ml 1.00 4 0.0580 0.6223 ppm 0.6 ppm 0.071 0.330 1.5 ml 3 ml 2.25 9 0.1065 0.9904 ppm 0.8 ppm 0.082 0.352 2.0 ml 4 ml 4.00 16 0.1640 1.4085 ppm 1.0 ppm 0.093 0.369 2.5 ml 5 ml 6.25 25 0.2325 1.845
∑ = 7.5
∑ = 15
= 55 

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