CNG NGH SINH HC TRONG TRNG HOA V CY CNH

Nhm 5: inh Danh Phc Nguyn Th Bch Tho Nguyn Th Thu Tho

I. Cc ng dng cng ngh sinh hc

1. Nhn ging cy trng bng nui cy m v t bo

Gi gn v nhn nhanh cc kiu gen qu Nhn nhanh cc loi, ging hoa, ging cy cnh kh trng bng ht Nhn nhanh kt hp vi lm sch virus, sch bnh Bo qun vn gen, tp on gen

2. Phc trng ging

Lm sch virus bng x l nhit v nui cy m phn sinh nh Vit Nam nghin cu thnh cng: phc hi ging hoa hu trng nhim bnh chai bng bng nui cy phn sinh m chi

3. Chuyn gen to ging hoa c mu sc mi

3 nhm antocyanin l dn xut ca pelargonidin, cyanidin, delphinidin S phi hp ca 3 nhm antocyanin to ra ph mu sc hoa rt rng Nhiu mu sc mi c to ra nh gen tng hp sc t t VSV v chuyn vo cc ging hoa

4. Mt s phng php khc

Bio-Protector: cht chit xut sinh hc cng ngh cao, tng sinh trng v tng khng cho cy
Khng mi i vi mi ngi Dng lng mu cnh gin nht Hu nh trong sut Khng cha tc nhn gy bnh

Bio-Protector Thnh phn ha hc

pH Cht hu c (acid humic, acid fulvic) m tng s Phospho tng s (25) Kali tng s (2) Ca Mg Fe Zinc (ppm) Manganese (ppm) 7,4 - 8,2 40-45% 1,2-2,5% 1,5-3,0% 1,7-2,4% 4,0-6,0% 0,6-2,3% 0,6-2,5% 108, 00 255, 00

Copper (ppm)
Boron (ppm)

9, 00
34, 00

Aluminum (ppm) Vi nm gy bnh

Trng giun, sn

12, 00 0.00
0.00

Bio-Protector
Enzyme: Dehydrogenase, Catalase, Urease, Alkalin phosphatase.. Cht iu ha sinh trng: IAA, acid gibberelic. Giu cc dng cht hu c cho cy

Li ch khi s dng Bio-Protector

Rt ngn thi gian ny mm Tng sc khng Cy sinh trng mnh Hn ch cy hp thu kim loi nng Duy tr nng sut sinh thi cao v n nh Tng nng sut v ci thin hng v ca sn phm nng nghip

Nc cm hoa sinh hc Bio-Pretty

Thch hp vi nhiu loi hoa Khng gy c v khng gy nhim mi trng Khng gy thi nc chng hoa

t sinh hc
Tc ng dinh dng Tc ng c l hc Tc ng vi sinh

t sinh hc
Thnh phn: N hu hiu: 0.5% P2O5 hu hiu: 0.3% K2O5 hu hiu: 0.7% pH: 6.37 EC: mn x l: S/cm 127 Acid Humic: 3.33% Trung-vi lng dng Cc loi vi sinh vt Vi nm khng bnh

t sinh hc
Ph hp cc loi t trng pha ct, pha st dng cho vn m, trang tri, hoa king Ci thin t bc mu, chng xi mn, xt l i ni

Gerbera jammesonii FERRARI nh vi khun Agrobacterium tumefaciens

Vt liu: - Gerbera jamesonii Ferrari - S dng vi khun chng EHA 105 mang vector ITB2c mang cc gen gus, bar, cryIAc. - Mi trng nui cy: MS, 30g/l sucrose, 10g/l gluco, 2mg/l BA, 0,3mg/l kinetin, 0,1mg/l IAA,2g/l phytagel, pH=5,4. - Chun b dch vi khun trc khi ly nhim: vi khun nui trong mi trng lng LB li tm 5000 vng/pht trong 5 pht sinh khi t bo vi khun pha trong mi trng pha long.

II. Chuyn gen vo cy hoa ng Tin

L X l sch Kh trng: CaOCl, 20-30 + Tween 20:1-2 git Ra sch bng nc ct

Nguyn liu sch

Ct nh hoc nghin Nui cy to m so Mi trng MS Mi trng chn lc b sung PPT 2,5mg/l 3,0ppm Cy bin i gen

Nhim mu vi VK

A.tumefacteciens

5 pht

Vt ra, kh Nui trong mi trng ng nui cy 48-72h, che ti, 240C

X l khng sinh dit VK b sung khng sinh Cefotaxime 500mg/l

Hnh: Chi ng tin trn mi trng chn lc bng PPT

Nui cy Dng X Sng Nai Phatycerum bifurcatum t bo t

L Dng X trng thnh

Thu bo t
Nc v trng

Ly tm 5 pht
Dung dch thuc ty 2 git ween 20

Ti huyn ph bo t
To chi Ly tm Nui trn mi trng nui cy III Cy con

Ti huyn ph trong 5mL nc ct

Loi b agar

Cho vo mi trng nui cy

Tch v nui cy (mi trng nui cy II)