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B.Tech-Minor subject-Biology Practical Paper-IV (Lab-III) IIIrd year-IInd semester (Elective-Industrial Biotechnology) 1. Fungal culture-Aspergillus 2.

Estimation of citric acid from Aspergillus culture 3. Screening and isolation of micro organisms, maintenance of strains and their improvement 4. Immunobilization of yeast cells 5. Production of Ethanol from yeast fermentation 6. Estimation of Alcohol by specific gravity method 7. Detection of amylase producing bacteria/Fungi from food sample 8. Estimation of fermentative product (Acetic acid from vinegar) 9. Determination of Biological oxygen Demand (BOD) of sewage sample or pond water 10.Bacterial examination of water by MPN method 11.Detection of coliforms for determination of the purity of potable water 12.Determination of dissolved oxygen concentration of water sample by Winklers method 13.Determination of chemical oxygen Demand (COD) of sewage sample or pond water

14.Preparation of Bacterial culture media (MS medium) and autoclaving (sterilization)

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